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1.
Adv Mater ; : e2201843, 2022 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-35509216

RESUMO

Spider dragline silk is drawn spun from soluble, ß-sheet-crosslinked spidroin in aqueous solution. This spider silk has an excellent combination of strength and toughness, which originates from the hierarchical structure containing ß-sheet crosslinking points, spiral nanoassemblies, a rigid sheath, and a soft core. Inspired by the spidroin structure and spider spinning process, we prepared a soluble and crosslinked nanogel and drew spun crosslinked fibers with spider-silk-like hierarchical structures containing cross-links, aligned nanoassemblies, and sheath-core structures. Introducing nucleation seeds in the nanogel solution, and applying pre-stretch and spiral architecture in the nanogel fiber, further tuned the alignment and assembly of the polymer chains, and enhanced the breaking strength (1.27 GPa) and toughness (383 MJ m-3 ) to approach those of the best dragline silk. Theoretical modeling provided understanding for the dependence of the fiber's spinning capacity on the nanogel size. This work provides a new strategy for the direct spinning of tough fiber materials. This article is protected by copyright. All rights reserved.

2.
J Agric Food Chem ; 2022 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-35579561

RESUMO

Emerging pesticide-resistant phytopathogenic bacteria have become a stumbling block in the development and use of pesticides. Quorum sensing (QS) blockers, which interfere with bacterial virulence gene expression, are a compelling way to manage plant bacterial disease without resistance. Herein, a series of isopropanolamine-decorated coumarin derivatives were designed and synthesized, and their potency in interfering with QS was investigated. Notably, compound A5 exhibited a better bioactivity with median effective concentration (EC50) values of 6.75 mg L-1 against Xanthomonas oryzae pv. oryzae (Xoo) than bismerthiazol (EC50 = 21.9 mg L-1). Further biochemical studies revealed that compound A5 disturbed biofilm formation and suppressed bacterial virulence factors and so forth. Moreover, compound A5 decreased the expression of QS-related genes. Interestingly, compound A5 had the acceptable control effect (53.2%) toward Xoo in vivo. Overall, this study identifies a novel lead compound for the development of bactericide candidates to control plant bacterial diseases by interfering with QS.

3.
FASEB J ; 36(6): e22341, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35579877

RESUMO

Multiple myeloma (MM), the terminally differentiated B cells malignancy, is widely considered to be incurable since many patients have either developed drug resistance or experienced an eventual relapse. To develop precise and efficient therapeutic strategies, we must understand the pathogenesis of MM. Thus, unveiling the driver events of MM and its further clonal evolution will help us understand this complicated disease. Chromosome 1 instabilities are the most common genomic alterations that participate in MM pathogenesis, and these aberrations of chromosome 1 mainly include copy number variations and structural changes. The chromosome 1q gains/amplifications and 1p deletions are the most frequent structural changes of chromosomes in MM. In this review, we intend to focus on the genes that are affected by chromosome 1 instability: some tumor suppressors were lost or down regulated in 1p deletions, and others that contributed to tumorigenesis were upregulated in 1q gains/amplifications. We have summarized their biological function as well as their roles in the MM pathogenesis, hoping to uncover potential novel therapeutical targets and promote the development of future therapeutic approaches.

4.
Nucleic Acids Res ; 2022 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-35580055

RESUMO

The development of RNA aptamers/fluorophores system is highly desirable for understanding the dynamic molecular biology of RNAs in vivo. Peppers-based imaging systems have been reported and applied for mRNA imaging in living cells. However, the need to insert corresponding RNA aptamer sequences into target RNAs and relatively low fluorescence signal limit its application in endogenous mRNA imaging. Herein, we remolded the original Pepper aptamer and developed a tandem array of inert Pepper (iPepper) fluorescence turn-on system. iPepper allows for efficient and selective imaging of diverse endogenous mRNA species in live cells with minimal agitation of the target mRNAs. We believe iPepper would significantly expand the applications of the aptamer/fluorophore system in endogenous mRNA imaging, and it has the potential to become a powerful tool for real-time studies in living cells and biological processing.

5.
Nat Biotechnol ; 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35501392

RESUMO

Many spatially resolved transcriptomic technologies do not have single-cell resolution but measure the average gene expression for each spot from a mixture of cells of potentially heterogeneous cell types. Here, we introduce a deconvolution method, conditional autoregressive-based deconvolution (CARD), that combines cell-type-specific expression information from single-cell RNA sequencing (scRNA-seq) with correlation in cell-type composition across tissue locations. Modeling spatial correlation allows us to borrow the cell-type composition information across locations, improving accuracy of deconvolution even with a mismatched scRNA-seq reference. CARD can also impute cell-type compositions and gene expression levels at unmeasured tissue locations to enable the construction of a refined spatial tissue map with a resolution arbitrarily higher than that measured in the original study and can perform deconvolution without an scRNA-seq reference. Applications to four datasets, including a pancreatic cancer dataset, identified multiple cell types and molecular markers with distinct spatial localization that define the progression, heterogeneity and compartmentalization of pancreatic cancer.

6.
BMC Med Inform Decis Mak ; 22(1): 124, 2022 May 06.
Artigo em Inglês | MEDLINE | ID: mdl-35524307

RESUMO

BACKGROUND: An emergency response to a medical situation is generally considered to be a risk decision-making problem. When an emergency event occurs, it makes sense to take into account more than one decision maker's opinions and psychological behaviors. The existing research tends to ignore these multidimensional aspects. To fill this literature gap, we propose a multi-attribute model. METHODS: The model is based on cumulative prospect theory (CPT), considering multiple experts' psychological factors. By not assuming full rationality, we extend existing models to allow multiple experts' risk preferences to be incorporated into the decision-making process in the case of an emergency. Then, traditional CPT is extended by allowing for multiple attributes. In addition, rather than using crisp data, interval values are adopted to tackle the usual uncertainties in reality. RESULTS: The multi-attribute CPT based model proposed can deal with the selection of potential emergency alternatives. The model incorporates interval values to allow more uncertainty and the comparative studies show that the optimal solution changes under different scenarios. CONCLUSIONS: Our illustrative example and comparative study show that considering multiple experts and multiple attributes is more reasonable, especially in complicated situations under an emergency. In addition, decision-makers' risk preferences highly affect the selection outcomes, highlighting their importance in the medical decision-making process. Our proposed model can be applied to similar fields with appropriate modifications.


Assuntos
Tomada de Decisão Clínica , Tomada de Decisões , Humanos , Incerteza
7.
Artigo em Inglês | MEDLINE | ID: mdl-35521682

RESUMO

BACKGROUND: The pathogenesis of Cushing's disease (CD) is still not adequately understood despite the identification of somatic driver mutations in USP8, BRAF and USP48. In this multiomics study, we combined RNA sequencing (RNA-seq) with Sanger sequencing to depict transcriptional dysregulation under different gene mutation backgrounds. Furthermore, we evaluated the potential of achaete-scute complex homolog 1 (ASCL1), a pioneer transcription factor, as a novel therapeutic target for treatment of CD and its possible downstream pathway. METHODS: RNA-seq was adopted to investigate the gene expression profile of CD, and Sanger sequencing was adopted to detect gene mutations. Bioinformatics analysis was used to depict transcriptional dysregulation under different gene mutation backgrounds. The function of ASCL1 in hormone secretion, cell proliferation and apoptosis were studied in vitro. The effectiveness of a ASCL1 inhibitor was evaluated in primary CD cells, and the clinical relevance of ASCL1 was examined in 68 patients with CD. RNA-seq in AtT-20 cells upon Ascl1 knockdown combined with published ChIp-seq data and dual luciferase assays were used to explore downstream pathways. RESULTS: ASCL1 was exclusively overexpressed in USP8-mutant and wild type tumors. Ascl1 promoted adrenocorticotrophin hormone overproduction and tumorigenesis and directly regulated Pomc in AtT-20 cells. A ASCL1 inhibitor presented promising efficacy in both AtT-20 and primary CD cells. ASCL1 overexpression was associated with a larger tumor volume and higher adrenocorticotrophin secretion in patients with CD. CONCLUSION: Our findings help to clarify the pathogenesis of CD and suggest that ASCL1 is a potential therapeutic target for treatment of CD.

8.
Sci Adv ; 8(19): eabn8555, 2022 May 13.
Artigo em Inglês | MEDLINE | ID: mdl-35544566

RESUMO

The Mars' climate is cold and dry in the most recent epoch, and liquid water activities are considered extremely limited. Previous orbital data only show sporadic hydrous minerals in the northern lowlands of Mars excavated by large impacts. Using the short-wave infrared spectral data obtained by the Zhurong rover of China's Tianwen-1 mission, which landed in southern Utopia Planitia on Mars, we identify hydrated sulfate/silica materials on the Amazonian terrain at the landing site. These hydrated minerals are associated with bright-toned rocks, interpreted to be duricrust developed locally. The lithified duricrusts suggest that formation with substantial liquid water originates by either groundwater rising or subsurface ice melting. In situ evidence for aqueous activities identified at Zhurong's landing site indicates a more active Amazonian hydrosphere for Mars than previously thought.

9.
BMC Genomics ; 23(Suppl 4): 360, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35546387

RESUMO

BACKGROUND: Genome-wide association studies (GWAS) have uncovered thousands of genetic variants that are associated with complex human traits and diseases. miRNAs are single-stranded non-coding RNAs. In particular, genetic variants located in the 3'UTR region of mRNAs may play an important role in gene regulation through their interaction with miRNAs. Existing studies have not been thoroughly conducted to elucidate 3'UTR variants discovered through GWAS. The goal of this study is to analyze patterns of GWAS functional variants located in 3'UTRs about their relevance in the network between hosting genes and targeting miRNAs, and elucidate the association between the genes harboring these variants and genetic traits. METHODS: We employed MIGWAS, ANNOVAR, MEME, and DAVID software packages to annotate the variants obtained from GWAS for 31 traits and elucidate the association between their harboring genes and their related traits. We identified variants that occurred in the motif regions that may be functionally important in affecting miRNA binding. We also conducted pathway analysis and functional annotation on miRNA targeted genes harboring 3'UTR variants for a trait with the highest percentage of 3'UTR variants occurring. RESULTS: The Child Obesity trait has the highest percentage of 3'UTR variants (75%). Of the 16 genes related to the Child Obesity trait, 5 genes (ETV7, GMEB1, NFIX, ZNF566, ZBTB40) had a significant association with the term DNA-Binding (p < 0.05). EQTL analysis revealed 2 relevant tissues and 10 targeted genes associated with the Child Obesity trait. In addition, Red Blood Cells (RBC), Hemoglobin (HB), and Package Cell Volume (PCV) have overlapping variants. In particular, the PIM1 variant occurred inside the HB Motif region 37,174,641-37,174,660, and LUC7L3 variant occurred inside RBC Motif region 50,753,918-50,753,937. CONCLUSION: Variants located in 3'UTR can alter the binding affinity of miRNA and impact gene regulation, thus warranting further annotation and analysis. We have developed a bioinformatics bash pipeline to automatically annotate variants, determine the number of variants in different categories for each given trait, and check common variants across different traits. This is a valuable tool to annotate a large number of GWAS result files.

10.
Nanomaterials (Basel) ; 12(9)2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35564202

RESUMO

A valley-Hall-like photonic insulator based on C3v Kagome split-ring is proposed. Theoretical analysis and numerical calculations illustrate that C3v symmetry can be broken not only by global rotation α but also individual rotation θ of the split rings, providing topological phase transitions. Furthermore, refraction of the edge state from the interface into the background space at Zigzag termination is explored. It is shown that positive/negative refraction of the outgoing beam depends on the type of valley (K or K'), from which the edge state is projected. These results provide a new way to manipulate terahertz wave propagation and facilitate the potential applications in directional collimation, beam splitting, negative refraction image, etc.

11.
Front Med (Lausanne) ; 9: 878075, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35572994

RESUMO

Introduction: Septic shock, largely caused by intestinal perforation, is the most common critical illness in intensive care unit (ICU). As an important quality control strategy in ICU, deep vein thrombosis (DVT) prevention is routinely used in the treatment of septic shock. Nevertheless, the effects of DVT prevention on septic shock are not fully revealed. This study was thus designed to investigate the effects of DVT prevention on septic shock caused by intestinal perforation in China. Methods: A total of 463 hospitals were enrolled in a survey, led by the China National Critical Care Quality Control Center (China NCCQC) from January 1, 2018 to December 31, 2018. The association between DVT prevention, including pharmacological prophylaxis and mechanical prophylaxis, and outcomes, such as prognosis, complications, hospital stays, and hospitalization costs, was determined in the present study. Main Results: Notably, the increased rates of DVT prevention were not associated with the onset of complications in patients with septic shock caused by intestinal perforation (p > 0.05). In addition, even though increased DVT prevention did not affect hospital stays, it significantly decreased the discharge rates without doctor's order in patients with septic shock caused by intestinal perforation (p < 0.05). Nevertheless, it should be noted that the rates of pharmacological prophylaxis but not mechanical prophylaxis were significantly associated with the costs of septic shock caused by intestinal perforation (p < 0.05). Although increased total rates of DVT prevention and the rates of mechanical prophylaxis did not reduce the mortality in patients with septic shock caused by intestinal perforation, the higher frequent intervention using pharmacological prophylaxis indicated the lower mortality of these patients (p < 0.05). Conclusions: DVT prevention by any means is a safe therapeutic strategy for treating septic shock caused by intestinal perforation, and pharmacological prophylaxis reduced the mortality of patients with septic shock caused by intestinal perforation.

12.
Endocr Connect ; 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35560299

RESUMO

INTRODUCTION: Most studies reporting posterior pituitary tumors (PPTs) are small case series or single cases. METHODS: Patients with a histological diagnosis of PPT from January 2010 to December 2021 in a tertiary center were identified. We reported clinical symptoms, endocrine assessments, radiological and pathological features, and surgical outcomes of PPTs. RESULTS: A total of 51 patients (23 males, 51.3 ± 10.3 years old) with PPT were included in this study. Major symptoms were visual defects, headache, and hypopituitarism, while diabetes insipidus was uncommon (9.8%). The typical radiological feature was homogeneous enhancement (84.3%) of a regular-shaped mass on T1 contrast imaging without cystic change, calcification, or cavernous sinus invasion. We achieved gross total resection in 38/51 patients (74.5%). Pathologically, all tumors showed TTF-1 immunoreactivity. Among 29 patients with suprasellar PPTs, postoperative hemorrhage due to tumor residue was encountered in 2/15 cases in the transcranial group and 0/14 in the endoscopy group. Patients with spindle cell oncocytoma (SCO) were more likely to be surgically treated (25% vs. 0%, P = 0.018), harbor a higher Ki-67 index (16.7% vs. 0% > 5% P = 0.050), and present a lower 2-year recurrence-free survival rate (67.5% vs. 90.9%) compared with patients with pituicytoma or granular cell tumor. CONCLUSION: PPTs should be considered in the differential diagnosis of patients with sellar and suprasellar masses with a regular lesion with homogeneous enhancement. SCOs had high proliferation activity and risk of recurrence.

13.
Pest Manag Sci ; 2022 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-35527698

RESUMO

BACKGROUND: The gradually aggravated disease caused by phytopathogenic bacteria severely restricts food security and crop yield, and few pesticides can relieve this severe situation. Thus, development and excavation of new agrochemicals with high bioactivity and novel action mechanism may be a feasible strategy to control intractable bacterial diseases. As the private molecular framework, steroid molecules exhibit diversiform bioactivities. Herein, a series of novel androst-4-ene derivatives were designed, synthesized, and investigated for their antibacterial behaviour to excavate novel agrochemicals on the base of steroid molecules. RESULTS: Bioassay results indicated that target compounds displayed high bioactivities toward three destructive phytopathogenic bacteria including Xanthomonas oryzae pv. oryzae (Xoo), Xanthomonas axonopodis pv. citri (Xac) and Pseudomonas syringae pv. actinidiae (Psa). Compound III19 displayed excellent in vitro antibacterial profiling (EC50  = 2.37 mg L-1 towards Xoo; EC50  = 2.10 mg L-1 towards Xac; EC50  = 9.50 mg L-1 towards Psa). Furthermore, compound III19 showed outstanding in vivo protective activities, with values of 81.81% and 58.75% towards kiwifruit bacterial canker and rice bacterial leaf blight, respectively. Analysis of the antibacterial mechanism disclosed that compound III19 enhanced host defence enzyme activities (SOD, POD, PAL, PPO, and CAT) and increased the salicylate synthase (SAS) content to induce host resistance. In addition, compound III19 increased the membrane permeability, destroyed the cell membrane, and killed the bacteria. CONCLUSION: Given these profiles of target compounds, we highlight a new strategy for controlling intractable plant bacterial diseases by inducing plant resistance and targeting the bacterial cell membrane. This article is protected by copyright. All rights reserved.

14.
Artigo em Inglês | MEDLINE | ID: mdl-35480987

RESUMO

There is growing need for a safe, efficient, specific and non-pathogenic means for delivery of gene therapy materials. Nanomaterials for nucleic acid delivery offer an unprecedented opportunity to overcome these drawbacks; owing to their tunability with diverse physico-chemical properties, they can readily be functionalized with any type of biomolecules/moieties for selective targeting. Nucleic acid therapeutics such as antisense DNA, mRNA, small interfering RNA (siRNA) or microRNA (miRNA) have been widely explored to modulate DNA or RNA expression Strikingly, gene therapies combined with nanoscale delivery systems have broadened the therapeutic and biomedical applications of these molecules, such as bioanalysis, gene silencing, protein replacement and vaccines. Here, we overview how to design smart nucleic acid delivery methods, which provide functionality and efficacy in the layout of molecular diagnostics and therapeutic systems. It is crucial to outline some of the general design considerations of nucleic acid delivery nanoparticles, their extraordinary properties and the structure-function relationships of these nanomaterials with biological systems and diseased cells and tissues.

15.
Chem Asian J ; 2022 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-35483968

RESUMO

The function of the CRISPR system can be conditionally controlled through rationally guided RNA engineering such that the target sequences can be precisely selected and manipulated. In particular, gRNA, as an important component of the CRISPR system, provides a unique tool for multifunctional control of the system based on the structure of the RNA itself. Therefore, we introduced here a protective group on the 2'-OH position of RNA to inhibit RNA-guided nucleic acid cleavage. Next, the modified gRNA can restore its original function under the chemical stimulation of hydrogen peroxide to realize the control of the CRISPR system. The experiment result demonstrated that the operating mechanism of this strategy may be based on chemical modifications that reduce the number of complementary base pairs between RNAs and targets, and the RNA-protein interaction. This further enriches the toolbox of conditional control of CRISPR function and has broad potential for gene editing in living cells and disease treatment using endogenous hydrogen peroxide.

16.
Artigo em Inglês | MEDLINE | ID: mdl-35381090

RESUMO

BACKGROUND: The relationship between maternal thyroid function and pregnancy outcomes remains controversial and the safe range of TSH is still unclear in women planning pregnancy. METHODS: This population-based cohort study enrolled Chinese women who became pregnant subsequently in 30 provinces from 2010 to 2012 from the National Free Preconception Checkups Project. The maternal TSH level within 6 months before pregnancy and different pregnancy outcomes were collected and analyzed using restricted cubic spline regression model for dose-response relationship and potential optimal cut-off values. Logistic regression was used to reveal the relationship between different TSH groups and the risk of adverse outcomes. RESULTS: Among 175112 women, a J-shaped association was revealed between TSH and LGA (P < 0.001). When TSH was lower than 1.27 or 0.91 mIU/L, lower TSH was associated with higher OR of LBW (P = 0.003) or preterm delivery (P < 0.001). There was no significant association of preconception TSH with SGA, macrosomia, fetal anomalies, stillbirth, natural or induced abortion, and cesarean delivery. The range of TSH for OR lower than 1.0 was within 0.91-1.82 mIU/L in dose-response association. Compared with TSH 0.91-1.82 mIU/L, TSH low (<0.40 mIU/L and 0.40-0.90 mIU/L) and high (1.83-2.49 mIU/L, 2.50-3.99 mIU/L and >4.00 mIU/L) were associated with higher risk of preterm delivery and LGA. There was no significant association between TSH groups and the risk of LBW except for TSH <0.40 mIU/L. CONCLUSION: Preconception TSH was associated with preterm delivery, LGA, and LBW. Preconception TSH had a bidirectional effect on LGA, indicating a potential mechanism regarding influence of TSH on birth weight. TSH within 0.91-1.82mIU/L was the potential safe range for preconception women.

18.
Artigo em Inglês | MEDLINE | ID: mdl-35437783

RESUMO

BACKGROUND: Left bundle branch area pacing (LBBAP) aims to capture the cardiac conduction system in area of the left bundle branch. Currently, LBBAP is mainly performed using lumen-less pacing leads (LLLs) with pre-shaped sheath. However, the data on LBBAP with stylet-driven leads (SDLs) without sheath is limited. OBJECTIVE: This study presents the feasibility, safety, and pacing characteristics of LBBAP using stylet-driven leads (SDLs) without the support of sheath. METHODS: A total of 25 patients with bradycardia indications who received LBBAP implantation with an attempt of SDL (FINELINE II 4471 lead, Boston Scientific, MA, US) between August 2020 and April 2021 at Sir Run Run Shaw Hospital were included in this retrospective cohort study. Twenty of them finally were paced with SDL in priority (SDL-LBBAP group). Twenty propensity score matching patients who underwent LBBAP with LLL (Select Secure 3830 lead, Medtronic, MN, US) and 20 right ventricular septal pacing (RVSP) with regular active fixation lead respectively in the same period (the LLL-LBBAP group and RVSP group) were compared using ECG characteristics, pacing parameters and complications during 6-month follow-up. RESULTS: LBBAP was successful with SDL in 23 of 25 patients (92%) and 20 of them were paced with SDL first. In the SDL-LBBAP group, the average age was 70.4 ± 8.2 years, and 55% of patients were male. Paced QRS duration and the stimulus to peak left ventricular activation time (Sti-LVAT) in SDL-LBBAP group were similar with those in LLL-LBBAP group and significantly shorter than those in RVSP group (126.1±14.1ms vs 124.8±10.9ms, P = 1.00; 77.7±11.2ms vs 73.5±9.3ms, P = 0.75; 126.1±14.1ms vs 147.7±22.5ms, P<0.001; 77.7±11.2ms vs 97.0±13.2ms, P<0.001). The pacing threshold and R-wave amplitude of SDL-LBBAP group were 0.53±0.18V and 11.53±3.63mV at baseline respectively, which were comparable with the other two groups. During the 6-month follow-up, the pacing parameters remained stable and no lead-related complication was recorded. CONCLUSION: It is feasible and safe to use stylet-directed pacing lead for permanent LBBAP without a delivery sheath. Similar to LLL, LBBAP using SDL showed stable parameters and narrower paced QRS duration compared with RVSP, which could be an alternative to LLL in LBBAP. This article is protected by copyright. All rights reserved.

19.
Res Q Exerc Sport ; : 1-9, 2022 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-35435811

RESUMO

Purpose: The preparation of Chinese physical education teachers focuses strongly on movement competence and the development of knowledge about rules and techniques. What is missing are experiences that promote expertise in task design and progressions. The purpose of this study was to examine if participation in classes following the Sport Education model could enhance content expertise by placing students in situations where they were responsible for these tasks. Methods: One hundred and ten physical education majors from a university in central China participated in a semester-long course of volleyball taught using either Traditional Instruction or Sport Education. Pre- and post-course measures were recorded of participants' game performance, common content knowledge (CCK), and specialized content knowledge (SCK). Results: After controlling for pre-intervention scores, statistically significant differences were evident in the posttest scores between the instructional groups for all three measures. The binomial logistic regression model to ascertain the effect of course type on the likelihood that students would reach the benchmark depth of SCK produced statistical significance. Students in the Sport Education classes had 6.67 times higher odds to reach the benchmark than students in the Traditional Instruction classes. Conclusion: The accountability mechanisms specific to Sport Education that have been shown to enhance student motivation and promote knowledge and performance seemed to carry over into this setting. As students in Sport Education were responsible for designing much of their team training, this served to promote their ability to design and sequence tasks based on their team's needs. Implications for physical education teacher education are discussed.

20.
J Agric Food Chem ; 70(16): 4899-4911, 2022 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-35437986

RESUMO

Bacterial biofilms are the root cause of persistent and chronic phytopathogenic bacterial infections. Therefore, developing novel agrochemicals that target the biofilm of phytopathogenic bacteria has been regarded as an innovative tactic to suppress their invasive infection or decrease bacterial drug resistance. In this study, a series of natural pterostilbene (PTE) derivatives were designed, and their antibacterial potency and antibiofilm ability were assessed. Notably, compound C1 displayed excellent antibacterial potency in vitro, affording an EC50 value of 0.88 µg mL-1 against Xoo (Xanthomonas oryzae pv. oryzae). C1 could significantly reduce biofilm formation and extracellular polysaccharides (EPS). Furthermore, C1 also possessed remarkable inhibitory activity against bacterial extracellular enzymes, pathogenicity, and other virulence factors. Subsequently, pathogenicity experiments were further conducted to verify the above primary outcomes. More importantly, C1 with pesticide additives displayed excellent control efficiency. Given these promising profiles, these pterostilbene derivatives can serve as novel antibiofilm agents to suppress plant pathogenic bacteria.


Assuntos
Infecções Bacterianas , Oryza , Xanthomonas , Antibacterianos/química , Antibacterianos/farmacologia , Biofilmes , Testes de Sensibilidade Microbiana , Oryza/microbiologia , Doenças das Plantas/microbiologia , Doenças das Plantas/prevenção & controle , Propanolaminas , Estilbenos
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