Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 293
Filtrar
1.
BMC Ophthalmol ; 22(1): 20, 2022 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-35016626

RESUMO

BACKGROUND: Myopia is a global public health issue. Controlling myopia progression is a primary focus of myopia studies today. Peripheral retinal myopic defocus is considered the mechanism for reduced myopia progression in orthokeratology studies. The topographic change in the front corneal surface after laser refractive surgery and orthokeratology procedures may appear similar. The purpose of this study was to explore the role of myopic laser ablation on axial length (AL) growth. METHODS: Myopic patients who underwent monocular excimer laser refractive surgery first in one eye and then in another eye several years later because of myopia occurrence or myopia progression were recruited. The axial length elongation and refraction (spherical equivalent) between the two eyes were observed and compared. RESULTS: A total of 8 myopic patients were enrolled in the study. The AL increased from 24.52 ± 0.96 mm to 24.68 ± 1.03 mm but without significance (T = 1.49, P > 0.05) in the ablated eyes. The AL increased significantly from 23.73 ± 0.91 mm to 24.26 ± 0.95 mm in the nonablated eyes (T = 6.76, P < 0.001). The AL elongation of the ablated eyes with 0.16 ± 0.30 mm growth was significantly lower than that of the nonablated eyes with 0.53 ± 0.32 mm growth (T = 8.98, P < 0.001). The spherical equivalent (SE) increased significantly in the ablated eyes (- 0.59 ± 0.21 (D), T = 6.36, P < 0.001) and in the nonablated eyes (- 0.97 ± 0.55 (D), T = 4.91, P < 0.01), and the difference between the two eyes was significant (T = 3.05, P < 0.05). CONCLUSIONS: The inhibitory effect of myopic laser ablation on AL elongation reported in the limited case studies argues for animal research on its efficacy as a new intervention for myopia progression.


Assuntos
Terapia a Laser , Miopia , Procedimentos Cirúrgicos Refrativos , Comprimento Axial do Olho , Humanos , Lasers de Excimer/uso terapêutico , Miopia/cirurgia , Refração Ocular , Estudos Retrospectivos
2.
Artigo em Inglês | MEDLINE | ID: mdl-35034070

RESUMO

PURPOSE: To determine the safety of intraocular pressure (IOP) measurement using a non-contact tonometer in patients who have undergone implantable collamer lens (ICL) implantation. SETTING: Eye Ear Nose and Throat Hospital. DESIGN: Prospective study. METHODS: Sixty-four eyes of 33 patients (aged 28.4±5.2 years) scheduled for ICL implantation were enrolled. The anterior segment parameters and vault were obtained using an anterior segment analyzer. A non-contact air-puff tonometer was employed before surgery, as well as 1 day and 1 week after surgery, to assess corneal biomechanical properties and intraocular pressure (IOP). Meanwhile, the Corvis ST was applied to evaluate the distance between the corneal endothelial layer and iris (E-Iris Dist), as well as the distance between the corneal endothelial layer and ICL (E-ICL Dist) when the cornea was deformed by the airflow. RESULTS: The mean anterior chamber depth (ACD), anterior chamber volume (ACV), and anterior chamber angle (ACA) decreased significantly after surgery (all P-values < 0.001). The E-ICL Dists measured 1 day and 1 week postoperatively were similar (P=0.107). However, a contact between the corneal endothelium and ICL was detected in two eyes when the cornea was deformed to the highest concavity. The 1 day postoperative vault, ACV, ACA, and preoperative SSI contributed significantly (P=0.001, P=0.023, P=0.010, and P=0.019, respectively) to determine the distance. The mean E-iris Dists decreased significantly at 1 day and 1 week after operation (P<0.001). The 1-week postoperative vault and ACD contributed significantly (P=0.025 and P=0.039, respectively) to determine the E-Iris Dist value. CONCLUSIONS: Excessively high vault, shallow ACD, narrow ACA, low ACV, and soft cornea are associated with a lower E-ICL distance, and thus may be risk factors for a contact between the corneal endothelium and ICL during IOP measurement. Although there is no proof that the contact may cause any risks to the endothelium, we recommend avoiding non-contact air-puff tonometry in eyes predicted by our model to be at risk of this occurring. Contact IOP measurements using goldmann applanation tonometers or dynamic contour tonometers may be alternate methods of IOP measurements in extreme cases.

3.
BMC Ophthalmol ; 22(1): 7, 2022 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-34980018

RESUMO

BACKGROUND: This study aimed to evaluate the clinical outcomes and assess preoperative characteristics that may predict outcomes in keratoconus 1 year after accelerated transepithelial corneal cross-linking (ATE-CXL). METHODS: This prospective study included 93 eyes of 84 consecutive keratoconus patients with 1-year follow-up after ATE-CXL. Preoperative characteristics included corneal astigmatism, anterior chamber depth, anterior chamber volume, radius of curvature, posterior elevation, central corneal thickness (CCT), thinnest corneal thickness, steepest meridian keratometry, flattest meridian keratometry, and the maximum keratometry (Kmax). Data were obtained preoperatively and at 1, 3, 6, and 12 months postoperatively. The patient eyes were grouped into 3 subgroups according to CCT and Kmax values to observe the changes of keratoconus progression. RESULTS: All patients were successfully operated without complications at any follow-up time point. Mean changes of Kmax from baseline at 6 and 12 months were - 0.60 ± 2.21 D (P = 0.011) and - 0.36 ± 1.58 D (P = 0.030), respectively. Eyes with a thinner CCT and higher Kmax values exhibited a tendency for topographic flattening of ≥1.0 D (P = 0.003; P = 0.003). In the subgroup comparison, the Kmax values decreased significantly at 6 and 12 months after ATE-CXL in the group with CCT ≤ 450 µm (P = 0.018 and P = 0.045); the Kmax values of the group with Kmax > 65.0 D decreased significantly at 6 months postoperatively (P = 0.025). CONCLUSION: ATE-CXL is a safe and effective treatment for keratoconus patients. Patients with thinner CCT and higher Kmax values are more likely to benefit from ATE-CXL.


Assuntos
Ceratocone , Fotoquimioterapia , Colágeno/uso terapêutico , Topografia da Córnea , Reagentes para Ligações Cruzadas/uso terapêutico , Seguimentos , Humanos , Ceratocone/tratamento farmacológico , Fármacos Fotossensibilizantes/uso terapêutico , Estudos Prospectivos , Riboflavina/uso terapêutico , Raios Ultravioleta , Acuidade Visual
4.
Ophthalmol Ther ; 11(1): 355-363, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34905159

RESUMO

INTRODUCTION: This study investigated the long-term visual and optical quality changes after small incision lenticule extraction (SMILE) for the correction of moderate to high myopia. METHODS: Forty-one eyes of 41 patients with a mean spherical equivalent (SE) of - 6.74 ± 1.45 D undergoing SMILE were enrolled. Preoperative and 1-month, 3-month, 1-year and 5-year postoperative follow-up examinations were performed including visual acuity, manifest refraction, corneal aberrations and contrast sensitivity. An optical quality analysis system was used to measure objective scatter index (OSI), Strehl ratio (SR) and modulation transfer function cutoff frequency (MTFcutoff). RESULTS: The safety and efficacy indices were 1.09 ± 0.12 and 1.03 ± 0.17, respectively, at the 5-year follow-up. No significant changes in SE from 1 month to 5 years postoperatively were found. The OSI significantly increased at 1 and 3 months postoperatively (p < 0.01 and p < 0.01) and declined at 1 and 5 years (p = 0.81 and p = 0.87). MTFcutoff and SR showed similar trends. Coma, spherical aberration and total higher-order aberration significantly increased after SMILE (p < 0.01) and remained stable from 1 month to 5 years after surgery (p > 0.05). Contrast sensitivity in the photopic and mesopic condition showed no significant changes during 5-year follow-up (p > 0.05). CONCLUSION: The SMILE procedure is satisfactory in terms of optical quality for correcting moderate and high myopia in the long-term period. TRIAL REGISTRATION: ChiCTR-ONRC-13003114.

5.
Front Med (Lausanne) ; 8: 764653, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34869472

RESUMO

Purpose: To compare the safety of the non-ophthalmic viscosurgical device (OVD) technique with that of the minimum OVD technique in EVO Implantable Collamer Lens (EVO-ICL) implantation. Methods: A total of 180 eyes of 90 consecutive patients were enrolled in the study, of which 100 eyes of 50 patients were treated with non-OVD technique, with a 55% success rate. The remaining 80 eyes of 40 patients were treated with min-OVD technique, so they were classified into the min-OVD group. Preoperative and postoperative intraocular pressure (IOP) measurements were collected and analyzed at 1, 2, 3, and 24 h. Visual acuity, corneal endothelial cell density (ECD), and corneal densitometry 24 h postoperatively were evaluated. Results: No significant difference was found in visual outcomes (P = 0.54) or ECD (P = 0.78) between the two groups. The operation time was significantly shorter in the non-OVD group (P < 0.0001). The IOP was significantly higher at 1 h (P < 0.0001), 2 h (P < 0.0001) and 3 h (P = 0.0045) postoperatively in the min-OVD group. The non-OVD group had significantly lower IOP than the min-OVD group at 1 h (P = 0.01) and 2 h (P = 0.013) postoperatively. The temporal corneal densitometry in the non-OVD group were significantly lower than those in the minimum group (P = 0.0063) 1 day after surgery. Conclusion: The non-OVD technique is safe and efficient for ICL implantation. It can be a safer method of ICL implantation in that it completely eliminates ophthalmic viscoelastic devices related complications without causing additional complications in short term.

6.
Front Bioeng Biotechnol ; 9: 772982, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34957070

RESUMO

Purpose: The purpose of this study was to establish a novel bilateral differential topographic algorithm and assess its efficacy for screening of keratoconus and corneal ectasia before corneal refractive surgery. Methods: One hundred and sixty-one consecutive patients (115 men and 46 women, aged 22.8 ± 6.8 years) with keratoconus, including clinical keratoconus, subclinical keratoconus, forme fruste keratoconus (FFK), and corneal ectasia (KC group) and one hundred and seventy-four consecutive patients (97 men and 77 women, aged 25.1 ± 6.7 years) with ametropia (control group) visiting the Eye and ENT hospital of Fudan University from June 2018 to April 2021 were included. Bilateral differential keratometry, elevation, and pachymetry topographies were composed based on raw topographic data obtained by a Scheimpflug imaging anterior segment analyzer. Key bilateral differential characteristic parameters were calculated. SPSS 20 (SPSS Inc., IBM) was used for statistical analyses and the receiver operating characteristic (ROC) curves were used to determine the diagnostic efficacies. Results: Mann-Whitney tests detected that the front keratometry, front elevation, corneal pachymetry, and back elevation maximal, mean, and standard deviation values within a 1.5-mm radius of the bilateral differential topography were all significantly higher in the KC group than in the control group (all p-values <0.001). The front keratometry mean (ΔFKmean) and standard deviation (ΔFKsd) and the front elevation standard deviation (ΔFEsd) and maximal (ΔFEmax) values within a 1.5-mm radius of the bilateral differential topography yielded the four highest accuracies (area under the ROC curve = 0.985, 0.985, 0.984, and 0.983, respectively) for discriminating KC cases (including FFK cases) from normal cases. Cut-off values of 0.75 diopters (D) for the ΔFKmean, 0.67 D for the ΔFKsd, 2.9 µm for the ΔFEsd, and 14.6 µm for the ΔFEmax had the highest sensitivities (95.7, 95.0, 96.9, and 95.0%, respectively) and specificities (96.0, 97.7, 94.8, and 95.4%, respectively). Conclusion: Bilateral differential topographic parameters may be efficient for the early detection of keratoconus and corneal ectasia secondary to corneal refractive surgery. This bilateral differential topographic algorithm may complement conventional diagnostic models by improving the sensitivity and specificity of screening for early keratoconus and ectasia before corneal refractive surgeries.

7.
Curr Eye Res ; : 1-7, 2021 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-34894946

RESUMO

PURPOSE: To investigate the long-term safety, efficacy, stability, vault, and complications of implantable collamer lens with (ICL V4c) and without (ICL V4) a central hole for correcting high myopia. METHODS: 78 eyes (40 patients) underwent ICL V4c implantation and 78 eyes (48 patients) underwent ICL V4 implantation were enrolled. They were followed up for 5 years of the uncorrected and corrected distance visual acuity, spherical equivalent (SE), axial length, intraocular pressure, endothelial cell density and vault. RESUITS: The safety indices of the ICL V4c and V4 groups at 5 years were 1.25 ± 0.40 and 1.31 ± 0.40, respectively (P = .353). The efficacy indices were 0.90 ± 0.29 and 1.00 ± 0.44, respectively (P = .098). The preoperative, 1 month and 5 year postoperative logMAR UDVAs were respectively 1.54 ± 0.48, 0.11 ± 0.17, and 0.20 ± 0.26 in the V4c group and 1.56 ± 0.44, 0.14 ± 0.19, and 0.22 ± 0.26 in the V4 group (P = .703, 0.329, 0.585). The logMAR CDVAs were resepectively 0.13 ± 0.18, 0.02 ± 0.11, and 0.05 ± 0.16 in the V4c group and 0.18 ± 0.22, 0.05 ± 0.14, and 0.09 ± 0.21 in the V4 group (P = .128, 0.169, 0.229). The SE were resepectively -15.10 ± 4.32 D, -0.71 ± 1.31 D and -1.65 ± 1.30 D in the V4c group and -15.44 ± 3.51 D, -0.61 ± 1.12 D and -1.40 ± 1.30 D in the V4 group (P = .585, 0.637, 0.296). The mean vault reduced by 97.31 ± 136.61 µm in the V4c group and by 99.74 ± 245.83 µm in the V4 group. Three eyes (3.85%) with mid-periphery anterior subcapsular opacification in the V4c group and two (2.56%) with cataract, one (1.28%) with central anterior subcapsular opacification in the V4 group were observed. The CDVA of 20/40 was obtained in the two cataract eyes after phacoemulcification and intraocular lens implantation. CONCLUSION: Long-term ICL V4c and ICL V4 implantations are safe, effective, and stable for high myopia correction. ICL V4c can potentially reduce the risk of lens opacification and may be more tolerant to low vault than ICL V4.

8.
Eye Vis (Lond) ; 8(1): 40, 2021 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-34753516

RESUMO

BACKGROUND: To evaluate the long-term safety, efficacy, predictability, and stability of implantable collamer lens with a central hole (EVO ICL) implantation for correcting high myopia (HM) and super high myopia (SHM). METHODS: This prospective study evaluated 83 eyes of 46 patients who were divided into groups based on their spherical equivalent refractive error (SE): HM group (- 12 D ≤ SE < - 6 D) and SHM group (SE < - 12 D). They were followed up for 5 years after ICL implantation; assessments of uncorrected distance visual acuity (UDVA), corrected distance visual acuity (CDVA), manifest refractive error, axial length, intraocular pressure, corneal endothelial cell density, and vault were conducted, and a questionnaire was administered. RESUITS: At 5 years postoperatively, the safety indices of the HM and SHM groups were 1.03 ± 0.10 and 1.32 ± 0.39, and the efficacy indices were 0.83 ± 0.25 and 0.86 ± 0.32, respectively. In the HM group, 60.47% and 79.07% of the eyes were within ± 0.50 D and ± 1.00 D of the attempted correction, while it was achieved for 22.50% and 47.50% of the eyes in the SHM group, respectively. The SE of the HM group decreased from - 9.72 ± 1.41 D preoperatively to 0.04 ± 0.39 D 1 month postoperatively and - 0.67 ± 0.57 D 5 years postoperatively, while in the SHM group, it decreased from - 15.78 ± 3.06 D preoperatively to - 0.69 ± 0.97 D 1 month postoperatively and - 1.74 ± 1.19 D 5 years postoperatively. CONCLUSION: EVO ICL implantation is safe, effective, and predictable for correcting HM and SHM. CDVA improved more after surgery for SHM, but the growth of axial length still needs attention.

9.
J Refract Surg ; 37(11): 734-740, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34756142

RESUMO

PURPOSE: To investigate 2-year visual outcomes, stability, and predictability after allogenic lenticule implantation in a 100-µm pocket for moderate to high hyperopia correction. METHODS: In this prospective case series, 14 eyes of 9 patients with moderate to high hyperopia ranging from +3.00 to +8.00 diopters sphere were included between March and September 2018. Allogenic lenticules extracted from myopic small incision lenticule extraction were implanted into a pocket created by femtosecond laser at a 100-µm depth in recipients with hyperopia. All patients were followed up for 2 years. Uncorrected (UDVA) and corrected (CDVA) distance visual acuity, manifest refraction, corneal topography, Fourier-domain optical coherence tomography, and in vivo confocal microscopy were examined. RESULTS: At postoperative 2 years, 2 eyes (14.3%) gained one line of CDVA, 11 eyes (78.6%) had unchanged CDVA, and 1 eye (7.1%) lost one line of CDVA. No eyes lost two or more lines of CDVA. Twelve of the treated eyes (85.7%) had postoperative uncorrected near visual acuity equal to or better than pre-operative values. The spherical equivalent decreased from +5.53 ± 1.45 D preoperatively to -0.60 ± 1.20 D at postoperative year 2 (P < .001). The anterior mean keratometric readings increased from 42.41 ± 1.03 D preoperatively to 48.38 ± 1.98 D at postoperative year 2 (P < .001). Of 14 treated eyes, 10 eyes (71.4%) had spherical equivalent within ±1.00 D. CONCLUSIONS: The findings suggest that allogenic lenticule transplantation may be a promising option for the correction of moderate to high hyperopia. [J Refract Surg. 2021;37(11):734-740.].


Assuntos
Hiperopia , Substância Própria/cirurgia , Topografia da Córnea , Humanos , Hiperopia/cirurgia , Refração Ocular , Resultado do Tratamento , Acuidade Visual
10.
Artigo em Inglês | MEDLINE | ID: mdl-34817750

RESUMO

PURPOSE: Older cancer patients are susceptible to long-term effects of chemotherapy, including cancer-related cognitive decline and impairments to quality of life. Taxane-based chemotherapies are associated with physical declines among older women and may negatively impact cognitive performance. We sought to examine whether changes in objective and subjective measures of cognitive performance and well-being differ among older breast cancer survivors as a function of taxane-based chemotherapy treatment regimens. METHODS: Individual-level data were pooled and harmonized from two large prospective studies of older (greater than 60 years) breast cancer survivors. Assessments were conducted prior to systemic therapy and up to 36 months after. Cognitive performance was assessed with objective (working memory, processing speed, and executive functions) and subjective tests and physical, emotional, and functional well-being were also assessed. RESULTS: One hundred and sixty-seven (M age = 67.3 years) women with 116 receiving chemotherapy with taxanes and 51 without taxanes contributed data. Declines in subjective cognition for both groups were significant between pre-treatment and 12-month follow-up. Significant improvements were seen on a measure of objective cognition (working memory) from 12 to 36 months. Measures of well-being improved from prior to systemic therapy to 12 months. Longitudinal changes across all measures did not vary as a function of receipt of taxane-based treatment. CONCLUSION: Older women who received treatment with taxanes did not have greater declines in cognitive performance or well-being than women receiving other chemotherapy regimens. Despite older cancer survivors being at greater risk for negative outcomes, treatment with taxane-based chemotherapies does not appear to exacerbate these health consequences.

11.
Lasers Surg Med ; 2021 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-34806777

RESUMO

PURPOSE: To conduct keratometry and investigate the ultrastructural changes after microwave thermokeratoplasty (MTK) in rabbit eyes. METHODS: Eighteen New Zealand rabbits (18 eyes) were recruited for this study. Ten eyes were chosen for slit-lamp photography and corneal topography at 1 day, 1 week, 1 month, 3 months, and 6 months postoperatively. Two rabbits were sacrificed on 1 day, 1 week, 1 month, and 3 months postoperatively. All remaining rabbits were sacrificed at 6 months postoperatively. The cornea was observed using hematoxylin and eosin staining and transmission electron microscopy. Data are expressed as mean ± standard deviation. p value was determined using repeated-measures analysis of variance. RESULTS: Corneal edema, disorganized corneal collagen fibers in the heated area, and necrotic fibroblasts were observed at 1 day and 1 week postoperatively. At 1 month postoperatively, corneal edema was not observed, and corneal cell morphology was normal. Moreover, corneal collagen fibers in the heated area shrunk and tended to be organized. The K1 and K2 values significantly decreased from 49.0 ± 1.2 D and 50.5 ± 0.9 D preoperatively to 40.3 ± 1.2 D and 43.2 ± 0.8 D 6 months postoperatively, respectively. The corneal thickness was 353.1 ± 9.3 µm preoperatively and 317.8 ± 27.7 µm at 6 months postoperatively; the difference was not statistically significant. CONCLUSIONS: Corneal keratometry showed flattening after MTK. Moreover, corneal collagen fibers in the heated area shrunk and tended to be organized at 6 months postoperatively. Further studies are required to determine the safety and stability of MTK.

12.
Crit Rev Food Sci Nutr ; : 1-12, 2021 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-34711083

RESUMO

Due to the multiple biological activities of polysaccharides, their great potential as "natural drugs" for many diseases has been the subject of continuous exploration in the field of food and nutrition. Apoptosis and autophagy play a key role in mammalian growth, development and maintenance of cellular homeostasis. Recent studies suggest that apoptosis/autophagy may be the key regulatory target for the beneficial effects of polysaccharides. However, the regulation of apoptosis and autophagy by polysaccharides is not consistent in different disease models. Therefore, this review outlined the relationship between apoptosis/autophagy and some common human diseases, then discussed the role of apoptosis/autophagy pathway in the regulation of human health by polysaccharides, Furthermore, the application of visualization, imaging and multi-omics techniques was proposed in the future trend. The present review may be beneficial to accelerate our understanding of the anti-disease mechanisms of polysaccharides, and promote the development and utilization of polysaccharides.

13.
Front Med (Lausanne) ; 8: 695697, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34595185

RESUMO

Significance: Our study found that SP-A1 (stiffness parameter at time of first applanation) was statistically different between post-laser-assisted subepithelial keratomileusis (LASEK) and post-small incision lenticule extraction (SMILE) eyes. The stiffness of keratoconus was lower than that of post-refractive surgery corneas. Purpose: To compare corneal biomechanics among post-SMILE, post-LASEK, and keratoconic eyes. Methods: In this retrospective study, 36 eyes of 36 patients after SMILE, 36 eyes of 36 patients after LASEK, and 36 eyes of 36 patients with keratoconus with matching (±5 µm) central corneal thickness (CCT) were examined using Scheimpflug corneal topography (Pentacam HR) and dynamic Scheimpflug analyzer (CorVis ST). Mixed linear model analysis with Bonferroni-adjusted post-hoc comparisons was performed to compare the differences in corneal biomechanics and topographic parameters among the three groups with the CCT and the bIOP (intraocular pressure with biomechanical correction) as the random factor. Results: All groups had comparable CCT at baseline. The bIOPs between the three groups were comparable. The mean values of the Belin/Ambrósio Deviation (BAD-D) in the keratoconus group were significantly higher than those of the SMILE (post hoc p < 0.001) and LASEK groups (post hoc p < 0.001). The SP-A1 in the keratoconus group was the lowest when compared with those of the SMILE (post hoc p = 0.003) and LASEK groups (post hoc p < 0.001). The SMILE group SP-A1 values were slightly lower than those of the LASEK group (post hoc p = 0.044). Conclusions: Keratoconus eyes were significantly softer when compared to post-refractive surgery corneas with comparable corneal thickness in terms of SP-A1 and BAD-D values, while the LASEK group may have the greatest stiffness. Post-SMILE and post-LASEK corneas showed significant differences in SP-A1.

14.
Transl Vis Sci Technol ; 10(12): 29, 2021 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-34665231

RESUMO

Purpose: To investigate the morphologic and histopathologic changes in allogeneic endokeratophakia using hyperopic lenticules derived from small-incision lenticule extraction (SMILE). Methods: Six New Zealand rabbits (12 eyes) were included in this experiment and randomly and evenly divided into donor and recipient groups. The donor group underwent bilateral hyperopic SMILE surgery, and the concave lenticules were implanted into eyes in the recipient group. Corneal topography and anterior segment optical coherence tomography (OCT) examinations were performed at 1 day, 1 week, 1 month, and 5 months after surgery. All eyes were enucleated 5 months after surgery. Hematoxylin and eosin (HE) staining and transmission electron microscopy (TEM) were used to observe the corneal morphology in the recipient group. Results: No complications were observed, and the corneas remained transparent in the follow-up period. There was mild corneal edema within 1 week after surgery. Slit-lamp microscopy and OCT showed that the lenticules were gradually integrated with the surrounding corneal stroma. HE staining showed that the arrangement of corneal collagen was regular. The boundary between the lenticules and surrounding tissue could be identified with HE staining and TEM, and no inflammatory cells were found under TEM. The corneal Km values were significantly lower at 5 months postoperatively compared to preoperatively (P < 0.05). Conclusions: This pilot study showed that allogeneic hyperopic SMILE lenticule endokeratophakia seems to be safe and feasible. Translational Relevance: Allogeneic hyperopic SMILE lenticule endokeratophakia may be applicable for the correction of corneal regression, ectasia, ultra-high myopia, or keratoconus.


Assuntos
Cirurgia da Córnea a Laser , Transplante de Células-Tronco Hematopoéticas , Hiperopia , Animais , Hiperopia/cirurgia , Lasers , Projetos Piloto , Coelhos
15.
Cont Lens Anterior Eye ; : 101517, 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34625345

RESUMO

PURPOSE: To investigate the relationship between myopia progression and axial length (AL) elongation in orthokeratology (ortho-k) patients. METHODS: This study investigated 184 patients (baseline age 9.0 ± 1.6 years), who underwent overnight ortho-k treatment for 12 to 72 months, and stopped lens wear for 1 to 2 months. Refractive sphere and cylinder after cycloplegia, corneal curvatures along both meridians, and AL were compared before the commencement and after discontinuation of ortho-k treatment. The effects of AL change, baseline AL, corneal curvature change, baseline age, and duration of ortho-k treatment on the change in spherical equivalent refractive error (SER) were analysed. RESULTS: Myopia significantly progressed and AL increased following 32.8 ± 13.0 months of ortho-k lens wear and 1 to 2 months washout period, as compared to baseline (all P < 0.001). Corneal curvature along the flat meridian (FK) became significantly flatter (P < 0.001) and corneal curvature along the steep meridian (SK) became steeper (P = 0.036). In the first stepwise multiple linear regression model (R2 = 0.696), the change in SER over time (ΔSER) is significantly correlated to the change in AL (ΔAL, P < 0.001), baseline AL (P < 0.001), baseline age (P = 0.028), change in SK (P = 0.002), and the duration of ortho-k lens treatment before discontinuation (P = 0.010). In a more simplified model (R2 = 0.628), the regression equation using ΔAL to predict ΔSER is: ΔSER = -0.094-1.608*ΔAL. CONCLUSIONS: The change in SER was significantly correlated to the change in AL, change in SK, baseline AL, baseline age, and the duration of treatment among children undergoing ortho-k therapy. The ratio of axial elongation to myopia progression was approximately 1:1.6 between the ages of 6 to 14 years. A simplified equation was derived for clinical use to estimate myopia progression from repeated AL measurement in ortho-k patients.

16.
Int J Ophthalmol ; 14(9): 1359-1364, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34540611

RESUMO

AIM: To investigate the safety and efficacy of using a one-step viscoelastic agent technique for posterior chamber phakic implantable collamer lens with a central hole (ICL V4c) implantation for myopia correction. METHODS: The one-step viscoelastic agent technique for ICL V4c implantation was used in 100 eyes of 52 patients. Refractive outcomes, intraocular pressure (IOP), corneal endothelial cell, and corneal densitometry values were evaluated at 1d, 1wk, 1 and 3mo postoperatively. RESULTS: All the surgeries were uneventful. No corrected distance visual acuity was lost after 3mo. IOP was 16.12±3.18 mm Hg before surgery, and 14.74±3.08 mm Hg at 1d and 14.50±2.56 mm Hg at 3mo after surgery (P<0.05). Corneal endothelial cell density was 2580±242 cell/mm2, the coefficient of variation in cell size was 42.11%±7.92%, and the percentage of hexagonal cells was 40.98%±9.46% before surgery. No significant difference was found when these outcomes were compared between the studied time points (P>0.05). The corneal densitometry values of the central 2 mm and 2 to 6 mm areas showed similar regularities. After surgery, the values significantly increased at 1d, then decreased to the preoperative values at 1wk, and then continued to decrease at 3mo (P<0.05). CONCLUSION: The one-step viscoelastic agent technique for ICL V4c implantation is found to be safe and effective for myopia correction and causes little disturbance to the cornea.

17.
Front Med (Lausanne) ; 8: 668762, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34513859

RESUMO

Purpose: To report the clinical outcomes of phototherapeutic keratectomy (PTK) for pediatric band keratopathy after treatment for retinoblastoma. Methods: A 5-year-old boy presented with a 2-year history of poor visual acuity and a horizontal gray-white band across the central cornea in the right eye. He was diagnosed with band keratopathy after chemo-laser-cryotherapy for retinoblastoma. The band keratopathy was treated via PTK using the Mel-90 excimer laser with an optical treatment zone of 7.0 mm and ablation depth of 120 µm. The patient was followed at 1 week and 3 months postoperatively. Results: Surgery and postoperative follow-up were uneventful. At the 3-month follow-up, the uncorrected distant visual acuity of the right eye improved to 20/125, and the corrected distance visual acuity improved to 20/70 with a refraction of +10.00 D/-2.50 DC × 15. The clarity of the ablated area was evidently improved. The central corneal thickness decreases from 612 to 584 µm. The optical coherence tomography showed the thin band of hyperreflectivity in the ablated area disappeared, corneal transparency improved and the corneal surface smoothened. Conclusions: PTK is a safe and effective procedure to treat band keratopathy following treatment of retinoblastoma in children. Early intervention can reduce the risk of developing deprivation amblyopia.

18.
Food Funct ; 12(21): 10524-10537, 2021 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-34569560

RESUMO

The release of lysosomal hydrolase into the cytoplasm is accompanied by several systems of apoptosis signal transduction, and the imbalance between cell viability and apoptosis induces tumorigenesis. Tea polysaccharides (TPs) are the main bioactive components in green tea with hopeful anti-tumor efficacy, while their mechanism is still unclear. Here, TPs significantly promoted the death of colon cancer cell line CT26. RNA-seq results showed that the signal pathways up-regulated by TPs included lysosome pathways, apoptosis, the release of mitochondrial pigment c and programmed cell death. Among them, the results of AO-EB and annexin V-FITC/PI double staining indicated that TPs significantly up-regulated apoptosis. In addition, TPs significantly disrupted the function of lysosomes, which would cause mitochondrial damage. Intriguingly, TPs treatment increased the expression of Bak1, cleaved caspase-9 and cleaved caspase-3, but decreased the level of Bcl-2 and mitochondrial membrane potential, which indicated that TPs induced mitochondrial-mediated apoptosis. Moreover, TPs ameliorated the reduced lysosomal numbers by Baf A1 (lysosomal inhibitor). Therefore, our data indicated that TPs targeted lysosomes and induced apoptosis by a lysosomal-mitochondrial pathway mediated caspase cascade, thereby inhibiting the proliferation of CT26 cells. In short, the data would help the development of TPs as potential cancer drug therapeutics.

19.
BMC Ophthalmol ; 21(1): 330, 2021 Sep 10.
Artigo em Inglês | MEDLINE | ID: mdl-34503464

RESUMO

BACKGROUND: To assess the effects of warm compress (WC) on tear film lipid layer, blink pattern and Meibomian gland function in patients with dry eye following femtosecond laser small incision lenticule extraction (SMILE) and laser-assisted subepithelial keratomileusis (LASEK). METHODS: We enrolled 37 eyes of 37 participants, each with dry eye for more than 2 years following SMILE (25 eyes) or LASEK (12 eyes). WC was performed using a spontaneously heating eye mask. Tear film break-up time (TBUT), tear film lipid layer thickness (TFLLT), blink pattern, Meibomian secretory function scores (MGS), visual acuity, spherical equivalent (SE), keratometry, central corneal thickness (CCT) and aberration were assessed before and after WC. RESULTS: After WC, the following mean values all increased relative to baselines: CCT, SE, minimum (Min-), maximum (Max-) and average (Ave-) TFLLT, TBUT, total MGS (TMGS), number of glands secreting any liquid (MGL), and complete blink rate (CBR) (p values ranging from < 0.001 to 0.042). Partial blink frequency (PBF) and partial blink rate (PBR) decreased (p = 0.002 in both cases). The decrease of PBF was higher in SMILE subgroup than in LASEK (p = 0.030). TBUT variation was positively correlated with that of Ave-TFLLT and TMGS (p = 0.046, 0.028, respectively). Max-TFLLT variation was correlated with that of TMGS (p = 0.020). CONCLUSIONS: WC may temporarily increase tear film thickness and stability, decrease partial blink, and partly augment Meibomian gland function in dry eye patients after corneal refractive surgeries. Future studies are required to investigate long term clinical efficacy and safety.


Assuntos
Síndromes do Olho Seco , Ceratectomia Subepitelial Assistida por Laser , Procedimentos Cirúrgicos Refrativos , Síndromes do Olho Seco/etiologia , Humanos , Glândulas Tarsais , Lágrimas
20.
Ophthalmol Ther ; 10(4): 1033-1044, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34559401

RESUMO

INTRODUCTION: To investigate nighttime symptoms in patients with myopic anisometropia after monocular small incision lenticule extraction (SMILE) surgery. METHODS: Thirty-six patients who had undergone monocular SMILE more than 6 months previously were recruited at the Eye & ENT Hospital of Fudan University. The average age at surgery was 25.4 ± 6.1 years. Preoperative spherical equivalent (SE) was -3.77 ± 1.56 D in SMILE-treated eyes and -0.08 ± 0.66 D in unoperated eyes. Main measurements included uncorrected and corrected distance visual acuity, manifest refraction, halo radius, contrast sensitivity, nighttime symptoms, and patient satisfaction. RESULTS: The mean follow-up time was 13.9 ± 3.4 months. The efficacy and safety indexes were 1.18 and 1.28, respectively. The halo radius was not significantly different between SMILE-treated and unoperated eyes under luminance conditions of 1, 5, and 100 cd/m2 (P = 0.055). No significant differences were observed in contrast sensitivity at all spatial frequencies between eyes under both uncorrected and corrected conditions (all P > 0.05). None of the patients reported moderate or severe symptoms at night. Mild symptoms (glare, halo, starburst) were reported and binocularly equal in 13 patients, whereas four patients reported better night vision in SMILE-treated eyes than unoperated eyes, and one of them experienced mild night vision disturbance. The overall satisfaction score was 9.39 ± 0.80. CONCLUSIONS: The disk halo size and contrast sensitivity in SMILE-treated eyes were similar to those in unoperated eyes, and nighttime symptoms almost completely resolved after SMILE.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...