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1.
Chemosphere ; 247: 125879, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31935575

RESUMO

A number of studies have reported that fine particulate matter (PM2.5) exposure is associated with adverse pregnancy outcomes. Moreover, PM2.5 exposure contributes to changes of gut microbiota. However, influences of PM2.5 exposure during gestation on maternal gut microbiota and pregnancy outcomes were not well understood. Here we performed a study using mice models. Dams were exposed to PM2.5 suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Pregnancy outcomes, maternal gut microbiota and short chain fatty acids on GD 18 were all measured. The fetal body weight of PM2.5 group was significantly lower than that of control group (p < 0.05). Meanwhile, the fetal body length of PM2.5 group was significantly shorter than that of control group (p < 0.05). The Shannon or Simpson index of PM2.5 group were higher than that of control group (p < 0.05). At the phyla level, compared to dams in control group, mice in the PM2.5 group had higher ratio of phyla Proteobacteria, Candidatus Saccharibacteria and Fusobacteria and lower ratio of phyla Acidobacteria, Gemmatimonadetes and Deferribacteres in the gut. Compared with control group, the concentration of isobutyric acid was higher in PM2.5 group, but butyric acid concentration was lower in PM2.5 group (p < 0.05). These findings suggested that prenatal exposure to PM2.5 had an effect on birth weight of fetus. Meanwhile, PM2.5 tracheal exposure during gestation caused changes in the distribution and structure of gut microbiota of dams.

2.
Otol Neurotol ; 2019 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-31821264

RESUMO

BACKGROUND: During skull base tumor surgery, temporomandibular joint (TMJ) dissection is commonly performed. The impact of this procedure on patients' postoperative diet and TMJ function is a matter of concern to surgeons. METHODS: We reviewed the Craniomandibular Index (CMI) for 32 patients (15 men and 17 women) who underwent TMJ dissection during surgery for skull base tumors between August 2015 and May 2018. RESULTS: Fifteen patients underwent removal of the mandibular condyle, and 17 had the condyle preserved. Twenty-one patients mainly underwent infratemporal fossa approach, and 11 underwent extended temporal bone resection. No significant difference between pre- and postoperative diet was observed in any group. Significant differences in CMI index were seen in all groups. The highest score was 0.115 of Dysfunction Index, observed postoperatively in the group that underwent condyle removal. CONCLUSIONS: For skull base tumor surgery, TMJ dissection has no significant impact on postoperative diet. Patients who underwent removal of the mandibular condyle have significantly worse postoperative TMJ function.

3.
Mol Nutr Food Res ; 63(24): e1900703, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31750605

RESUMO

SCOPE: Diabetes endangers health and causes serious economic impediment. The aim of this study is to identify the effects of goat milk consumption on glucose metabolism of rats with high-fat (HF) diet. METHODS AND RESULTS: Sixty male Sprague Dawley rats are divided into five groups and fed with different diets for 24 weeks: goat-milk-based HF diet (GHF group; goat milk powder+HF diet), cow-milk-based HF diet (CHF group; cow milk powder+HF diet), HF diet, HF diet plus acarbose (HF+A group; acarbose+HF diet), and chow diet (CD group). Fasting glucose in GHF-fed rats are lower than HF-fed rats on weeks 16 and 20. GHF-fed rats display improved insulin sensitivity in oral glucose and insulin tolerance tests. Compared with HF-fed rats, glycated hemoglobin and triglycerides in GHF-fed rats are lower and high-density lipoprotein level is higher. AMP-activated protein kinase activation (AMPK) in the liver and skeletal muscle is higher in GHF rats than HF rats. Phosphoenolpyruvate carboxykinase and glucose 6-phosphatase protein levels in the liver are lower and hexokinase 2 protein level in the skeletal muscle is higher in GHF rats compared with HF rats. CONCLUSION: Goat milk consumption can ameliorate abnormalities in glucose metabolism, and AMPK pathway in the liver and skeletal muscle plays an important role in the process.

4.
Artigo em Inglês | MEDLINE | ID: mdl-31717430

RESUMO

Short chain fatty acids (SCFAs) are produced by the colonic microbiota through fermentation. Influences of maternal PM2.5 exposure on SCFAs of the offspring have not been well understood. Additionally, studies of dietary intervention have not been carried out yet. Here we performed a study that dams were received PM2.5 and quercetin intervention during gestation and lactation. SCFAs in colon of dams and their offspring (on postnatal day 21 and 35) were analyzed using gas chromatography. For male offspring, when compared with the control group levels of acetic acid, butyric acid, and valeric acid were lower in the PM2.5 group (p < 0.05), however, levels of isobutyric acid and isovaleric acid were higher in the PM2.5 group (p < 0.05). For female offspring, as compared with the control group, propanoic acid was lower in the PM2.5 group, however isovaleric acid was higher in the PM2.5 group (p < 0.05). 100 mg/kg and 200 mg/kg quercetin intervention could inhibit SCFAs production of male offspring, especially in isobutyric acid and isovaleric acid (p < 0.05). 100 mg/kg quercetin intervention could upgrade the level of propanoic acid of female offspring (p < 0.05). Taken together, these results suggest that PM2.5 tracheal exposure during gestation and lactation could influence SCFAs of offspring. Quercetin administration might have the potential to offset the effects of mater PM2.5 exposure on SCFAs in the offspring to some extent. The above effects were showed in a sex-dependent manner.

5.
Antioxidants (Basel) ; 8(11)2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31683564

RESUMO

White peony is a type of white tea (Camellia sinensis) rich in polyphenols. In this study, polyphenols were extracted from white peony. In vitro experiments showed that white peony polyphenols (WPPs) possess strong free radical scavenging capabilities toward 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Long-term alcohol gavage was used to induce alcoholic liver injury in mice, and relevant indices of liver injury were examined. WPPs effectively reduced the liver indices of mice with liver injury. The serum levels of aspartate aminotransferase (ATS), alanine aminotransferase (ALT), alkaline phosphatase (ALP), triglycerides (TG), total cholesterol (TC), blood urea nitrogen (BUN), nitric oxide (NO), and malondialdehyde (MDA) were downregulated, while those of albumin (ALB), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were upregulated. WPPs also reduced the serum levels of interluekin-6 (IL-6), interluekin-12 (IL-12), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ) in mice with liver injury. Pathology results showed that WPPs reduced alcohol-induced liver cell damage. Quantitative polymerase chain reaction (qPCR) and western blot results revealed that WPPs upregulated the mRNA and protein expressions of neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), manganese superoxide dismutase (Mn-SOD), cupro-zinc superoxide dismutase (Cu/Zn-SOD), and CAT and downregulated iNOS expression in the liver of mice with liver injury. WPPs protected against alcoholic liver injury, and this effect was equivalent to that of silymarin. High-performance liquid chromatography revealed that WPPs mainly contained the polyphenols gallic acid, catechinic acid, and hyperoside, which are critical for exerting preventive effects against alcoholic liver injury. Thus, WPPs are high-quality natural products with liver protective effects.

6.
BMC Public Health ; 19(1): 1076, 2019 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-31399027

RESUMO

BACKGROUND: Chronic inflammation contributes to the risk of osteoporosis and fracture. Dietary Inflammatory Index (DII), a novel method appraising the inflammatory potential of diet, has been utilized to examine the association between diet and bone health among postmenopausal women or the elderly. However, its relationship with bone density (BD) in lactating women has not been studied. METHODS: The prospective study was conducted to assess the possible association between DII and maternal BD during lactation. We enrolled 150 lactating women in the cohort. Participants were measured ultrasonic BD as baseline values at 1 month postpartum. After five-month follow up, the participants' BD were measured again. DII scores were calculated from semi-quantitative food frequency questionnaires (FFQ) and divided into tertiles. We compared the differences in the changes of BD at 6 months postpartum without or with adjustment for potential covariates across the tertiles. RESULTS: The women in Q1 of DII scores had less bone mass loss than those in Q2 and Q3 without adjustment for any covariates (p < 0.01); after adjusting demographic characteristics such as BMI (kg/m2) at 6 months postpartum, educational level, metabolic equivalent (MET), daily energy intake (kcal/d), we found that participants in the highest tertile of DII scores had much more bone loss than those in the lowest tertile (p = 0.038). However, in the test for trend, no significant association between DII and the changes of maternal BD at 6 months postpartum was observed. CONCLUSIONS: Chinese lactating women with higher DII scores have more bone mass loss; however significant differences and trends are attenuated and/or disappear depending on covariates and confounders that are taken into account in statistical analysis. The further study should be conducted in larger population to explore whether the significant association between DII and BD exists in Chinese lactating women.


Assuntos
Densidade Óssea/fisiologia , Dieta/efeitos adversos , Inflamação/fisiopatologia , Lactação/fisiologia , Adulto , China , Feminino , Humanos , Estudos Longitudinais , Período Pós-Parto , Estudos Prospectivos
7.
Mar Drugs ; 17(7)2019 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-31284571

RESUMO

Ochrazepines A-D (1-4), four new conjugates dimerized from 2-hydroxycircumdatin C (5) and aspyrone (6) by a nucleophilic addition to epoxide, were isolated from the fermentation broth of the coral-associated Aspergillus ochraceus strain LCJ11-102. Their structures including absolute configurations were determined based on spectroscopic analysis and chemical methods. Compounds 1-4 were also obtained by the semisynthesis from a nucleophilic addition of 2-hydroxycircumdatin C (5) to aspyrone (6). New compound 1 exhibited cytotoxic activity against 10 human cancer cell lines while new compounds 2 and 4 selectively inhibited U251 (human glioblastoma cell line) and compound 3 was active against A673 (human rhabdomyoma cell line), U87 (human glioblastoma cell line), and Hep3B (human liver cancer cell line) with IC50 (half maximal inhibitory concentration) values of 2.5-11.3 µM among 26 tested human cancer cell lines.


Assuntos
Antozoários/microbiologia , Aspergillus ochraceus/química , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Fermentação/fisiologia , Humanos , Concentração Inibidora 50 , Pironas/química , Pironas/farmacologia
8.
Eye (Lond) ; 33(10): 1606-1612, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31065104

RESUMO

PURPOSE: To investigate the alterations of trace elements levels in aqueous humor of patients with cytomegalovirus retinitis (CMVR). METHODS: A total of 15 eyes of 11 patients with CMVR and 24 eyes of 24 patients with senile cataract as control group were enrolled. Aqueous humor samples were assessed for calcium (Ca), potassium (K), magnesium (Mg), sodium (Na), phosphorus (P), titanium (Ti), vanadium (V), chromium (Cr), manganese (Mn), iron (Fe), nickel (Ni), copper (Cu), zinc (Zn), selenium (Se), strontium (Sr), and lead (Pb) by using inductively coupled-plasma-mass-spectrometry. Meanwhile, we examined the concentration of the CMV DNA load by using PCR and the concentration of interleukin (IL)-8 by using a cytometric bead array. RESULTS: In patients with CMVR, the aqueous humor levels of P and Cu were significantly higher than those of controls (p < 0.001, p < 0.001, respectively). However, levels of K and Mg were significantly lower in patients with CMVR (p < 0.001, p < 0.001, respectively). The Spearman correlation test showed that the concentration of IL-8 in the aqueous humor was significantly associated with the aqueous level of Cu (p = 0.009, r = 0.646) and Se (p = 0.031, r = 0.558). In addition, the concentration of CMV DNA load in the aqueous humor was significantly associated with the aqueous level of Ca (p = 0.027, r = -0.568), Mn (p = 0.020, r = 0.593), and Cu (p = 0.043, r = 0.527). CONCLUSIONS: Our preliminary results demonstrated that the abnormal aqueous levels of trace elements (P and Cu) in CMVR patients. Thus, the roles of trace element changes in the development of CMVR and the influence of intraocular trace element for the prognosis of CMVR warrant further investigations.

9.
BMC Pregnancy Childbirth ; 19(1): 172, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-31092215

RESUMO

BACKGROUND: Pregnant and lactating women are at high risk of insufficient water intake. The cross-sectional study was mainly designed to evaluate the water intake, including total water intake (TWI), plain water intake, and water intake from beverages and foods of 200 pregnant women and 150 breastfeeding women in Beijing. METHODS: A semi-quantitative Food Frequency Questionnaire (FFQ) was employed to assess their dietary intake, TWI, plain water, and water intake from beverages and foods. Multivariate regression analysis was conducted for evaluating the association between water intake and dietary variables. RESULTS: On average, the TWI of pregnant and breastfeeding women was 2638 mL/day and 3218 mL/day, respectively. Only 28% of pregnant women and 27% of breastfeeding women were complied with the adequate intake (AI). Water from foods was the greatest contributor to TWI both in pregnant and breastfeeding women. TWI was positively related to some dietary variables (P < 0.001). For pregnant women, with each 100 kcal/day increase in energy intake, the TWI increased by 67 mL. With each 5 g increase in daily intake of dietary protein, fat, carbohydrate and fiber, TWI increased by 72 mL, 66 mL, 22 mL, 353 mL, respectively. When the energy contribution of protein increased by 5%, TWI increased by 210 mL. The each 100 mg increase in daily sodium intake was accompanied with 52 mL increase in TWI. For breastfeeding women, with each 100 kcal/day increase in energy intake, the TWI increased by 54 mL. With each 5 g increase in daily intake of dietary protein, fat, carbohydrate and fiber, TWI increased by 53 mL, 58 mL, 16 mL, 212 mL, respectively. The each 100 mg increase in daily sodium intake was accompanied with 54 mL increase in TWI. CONCLUSIONS: A large proportion of pregnant and breastfeeding women in Beijing were not adherent to AI for TWI set by Chinese Nutrition Society. Water intake from foods was the greatest contributor to TWI both in pregnant and breastfeeding women, and maternal dietary intake posed impacts on water intake during pregnancy and lactation. More researches are required to assess the water intake and hydration status of the populations.


Assuntos
Aleitamento Materno , Alimentos , Água/administração & dosagem , Adulto , Pequim , Bebidas , Estudos Transversais , Carboidratos da Dieta/administração & dosagem , Gorduras na Dieta/administração & dosagem , Fibras na Dieta/administração & dosagem , Proteínas na Dieta/administração & dosagem , Ingestão de Líquidos , Ingestão de Energia , Feminino , Humanos , Cooperação do Paciente , Gravidez , Recomendações Nutricionais , Sódio na Dieta/administração & dosagem , Adulto Jovem
10.
Chemosphere ; 213: 182-196, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30218877

RESUMO

Epidemiological studies show that maternal exposure to PM2.5 affects the neurodevelopment of the offspring, especially the neurocognitive function. However, no relevant experimental researches have been published on toxic mechanism and diet intervention. We evaluated the effects of exposure to different doses of PM2.5 on the behavioral development of offspring via a PM2.5 exposure model established by intratracheal instillation, explored its mechanism and the protective effects of quercetin and VC intervention, and focused on the protein expression of CREB/BDNF signaling pathway. Specifically, Exposure to PM2.5 during gestation and lactation period caused maternal oxidative stress. Maternal exposure to PM2.5 changed postnatal open-field behaviors in both gender, impaired spatial learning and memory in the female offspring, increased the level of IL-1ß, IL-6, down-regulated p-CREB/CREB, BDNF, TrkB, p-CaMKII/CaMKII, p-CaMKIV/CaMKIV, up-regulated p-Akt/Akt and p-ERK1/2/ERK1/2 in the offspring. In addition, maternal supplementation with quercetin ameliorate the maternal oxidative stress, improved progeny inflammatory response, regulated BDNF, TrkB, p-Akt/Akt, p-ERK1/2/ERK1/2 in female offspring, regulated TrkB, p-CREB/CREB and p-Akt/Akt in male offspring. Maternal supplementation with VC increased the levels of CAT in maternal mice, up-regulated BDNF in female offspring, regulated p-CREB/CREB and p-ERK1/2/ERK1/2 in male offspring. Our findings indicate that PM2.5 exposure during pregnancy and lactation could impair behavioral development of offspring. Quercetin shows more protective effects than VC. The mechanism of neurodevelopmental toxicity induced by PM2.5 may be related to oxidative stress, inflammatory response and modulation of the CREB/BDNF signaling pathway.


Assuntos
Ácido Ascórbico/química , Transtornos do Neurodesenvolvimento/induzido quimicamente , Quercetina/química , Animais , Feminino , Masculino , Exposição Materna , Camundongos , Transtornos do Neurodesenvolvimento/patologia , Gravidez
11.
Molecules ; 23(2)2018 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-29370108

RESUMO

Polysaccharides are closely associated with immune regulation, but there are different polysaccharide effects from different sources. In this study, the aim was to investigate the effect of tremella polysaccharides (TP) in cyclophosphamide-induced immunodeficient mice. We observed the thymus and spleen index, liver and spleen pathological changes, and the levels of IL-2, IL-12, INF-γ, TGF-ß and Ig G in serum, and we also noted the mRNA expression of IL-1ß, IL-4, IL-12 and TGF-ß in liver and spleen. Besides, we also measured the best effects of different doses of TP (Low-TP was 20 mg/kg·BW, Middle-TP was 40 mg/kg·BW, and High-TP was 80 mg/kg·BW) on cyclophosphamide-induced immunosuppressed mice. The results were remarkable, and suggested that TP had a significant effect for enhancing immunity in cyclophosphamide-induced immunosuppression, and the immune enhancement of High-TP had the best results in TP-treated mice. It could significantly increase the thymus and spleen index, alleviate pathological features of immunosuppression such as the arrangement of liver sinusoid and hepatic plates was disordered, massive inflammatory cells infiltrated and fatty degeneration of hepatocytes in liver, and red pulp and white pulp were intermixed, splenic corpuscles demolished and disappeared, splenic sinusoid extended, and lymphocytes of spleen were reduced in spleen. Besides, it could also up-regulate serum levels of IL-2, IL-12, INF-γ and Ig G, reduce the level of TGF-ß in serum, markedly promote mRNA expression of IL-1ß, IL-4 and IL-12 in liver and spleen, and suppress mRNA expression of TGF-ß. Above all, TP showed preventive effect for cyclophosphamide-induced immunosuppressed mice.


Assuntos
Basidiomycota/química , Ciclofosfamida/farmacologia , Polissacarídeos Fúngicos/farmacologia , Fatores Imunológicos/farmacologia , Imunomodulação/efeitos dos fármacos , Animais , Biomarcadores , Ciclofosfamida/efeitos adversos , Citocinas/sangue , Citocinas/genética , Feminino , Polissacarídeos Fúngicos/química , Perfilação da Expressão Gênica , Regulação da Expressão Gênica/efeitos dos fármacos , Imunoglobulina G/sangue , Fatores Imunológicos/química , Tecido Linfoide/efeitos dos fármacos , Tecido Linfoide/imunologia , Tecido Linfoide/metabolismo , Masculino , Camundongos
12.
Arch Virol ; 163(3): 787-790, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29188364

RESUMO

The complete genomic sequence of a novel potyvirus was determined from Paris polyphylla var. yunnanensis. Its genomic RNA consists of 9,660 nucleotides (nt) excluding the 3'-terminal poly (A) tail, containing the typical open reading frame (ORF) of potyviruses and encoding a putative large polyprotein of 3030 amino acids. The virus shares 53.9-70.1% nt sequence identity and 43.9-73.2% amino acid sequence identity with other viruses classified within the genus Potyvirus. Proteolytic cleavage sites and conserved motifs of the potyviruses were identified in the polyprotein and within individual proteins. Phylogenetic analysis indicated that the virus is most closely related to members of the BCMV subgroup. The results suggest that the virus should be classified as a novel species within the genus Potyvirus, which we tentatively name "Paris mosaic necrosis virus".


Assuntos
Genoma Viral , Melanthiaceae/virologia , Filogenia , Potyvirus/genética , RNA Viral/genética , Sequência de Aminoácidos , Tamanho do Genoma , Sequenciamento de Nucleotídeos em Larga Escala , Fases de Leitura Aberta , Doenças das Plantas/virologia , Poliproteínas , Potyvirus/classificação , Potyvirus/isolamento & purificação , Homologia de Sequência de Aminoácidos
13.
Biochim Biophys Acta Mol Basis Dis ; 1863(11): 2954-2963, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28847511

RESUMO

We previously demonstrated that histamine H4 receptor (HRH4) played important roles to suppress epithelial-to-mesenchymal transition (EMT) progress in non-small cell lung cancer (NSCLC). Furthermore, recent investigations suggested that genetic variations in HRH4 gene affected HRH4 function and eventually contributed to certain HRH4-related diseases. However, the relations between polymorphisms in HRH4 gene and NSCLC as well as their underlying mechanisms remain largely uninvestigated. This study aims to investigate the genetic effect of a nonsynonymous HRH4 polymorphism (rs11662595) on HRH4 function and its association with NSCLC both basically and clinically. For basic experiments, A549 cells were transfected with either wild type or rs11662595 mutated HRH4 clone and subjected to both in vitro and in vivo experiments. We showed that rs11662595 significantly decreased the ability of HRH4 to activate Gi protein, which resulted in facilitation of EMT progress, cell proliferation, and invasion behavior in vitro. Moreover, in vivo experiments also showed that rs11662595 attenuated the anti-EMT effects of HRH4 agonist in inoculated nu/nu mice. For clinical experiments, we performed a prospective cohort study among 624 NSCLC patients and further proved that rs11662595 was responsible for the prognosis, degree of malignancy and metastasis of NSCLC. In conclusion, these findings reveal that rs11662595 is a loss-of-function polymorphism that results in dysfunction of HRH4 and attenuates the anti-EMT function of HRH4 in NSCLC, which provides a promising biomarker for prognosis and therapy of NSCLC.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/metabolismo , Transição Epitelial-Mesenquimal , Neoplasias Pulmonares/metabolismo , Mutação , Proteínas de Neoplasias/metabolismo , Receptores Histamínicos H4/metabolismo , Células A549 , Animais , Carcinoma Pulmonar de Células não Pequenas/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Feminino , Humanos , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Masculino , Camundongos , Camundongos Nus , Proteínas de Neoplasias/genética , Polimorfismo Genético , Estudos Prospectivos , Receptores Histamínicos H4/genética
14.
Artigo em Inglês | MEDLINE | ID: mdl-28574437

RESUMO

The objective is to investigate the influence of PM2.5 exposure on peripheral blood lymphocyte subsets in pregnant mice and the antagonism of quercetin on adverse effects induced by PM2.5 exposure. Pregnant mice were randomly divided into control group, PM2.5 model group and 3 quercetin intervention groups. Dams in all groups except the control group were exposed to PM2.5 suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Meanwhile, each dam was given 0.15% carboxymethylcellulose sodium (CMCS) (control group & PM2.5 model group) and different doses of quercetin (quercetin intervention groups) by gavage once a day from GD0 to GD17. The percentage of lymphocyte subsets, Biomarkers of systemic inflammation injuries (IL-2, IL-6, IL-8 & TNF-α) and oxidative stress indicators (CAT, GSH & HO-1) in peripheral blood of the dams were analyzed. The number of T cells increased, accompanied by increased level of IL-2, IL-6, IL-8 and HO-1 due to PM2.5 exposure. Less CD4+ and CD8+ T cells were counted in 100 mg/kg quercetin intervention group, compared with PM2.5 model group. Quercetin may inhibit cytokine production, especially in IL-6 and IL-8 and may upgrade the level of HO-1. Our findings indicate that PM2.5 could significantly influence the distribution of T-lymphocyte subsets, activate inflammatory reaction and elevate oxidative stress level in peripheral blood of pregnant mice. Certain dose of quercetin administration during pregnancy may protect the dams against the adverse effects through various ways.


Assuntos
Inflamação/induzido quimicamente , Estresse Oxidativo/efeitos dos fármacos , Material Particulado/efeitos adversos , Quercetina/farmacologia , Animais , Biomarcadores/metabolismo , Citocinas/genética , Citocinas/metabolismo , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Inflamação/metabolismo , Subpopulações de Linfócitos/efeitos dos fármacos , Camundongos , Gravidez , Substâncias Protetoras/farmacologia , Distribuição Aleatória
15.
Biomed Mater ; 12(1): 015012, 2016 12 09.
Artigo em Inglês | MEDLINE | ID: mdl-27934783

RESUMO

In situ injectable double-crosslinked hydrogels containing thiol functionalized poly(amido-amine) dendrimers (Gn-PAMAM-NH2-X) and oxidized dextrans (ODex) were prepared under physiological conditions without using potentially cytotoxic cross-linkers. The double-crosslinked structure was created by Schiff's base reaction and the formation of disulfide bonds. The morphology of the hydrogels was characterized by scanning electron microscopy. The gelation time, swelling and rheological behaviors of the hydrogels were investigated. We also studied the adhesive strength and cytocompatibility of the hydrogels. The surface amino density, concentration and generation of PAMAM are the main factors affecting the gelation. Relatively high surface amino density contributes to quick gelation, whereas too great a surface amino may lead to the brittleness of the hydrogel. A moderate concentration of PAMAM (10% wt) is suitable for gelation considering its appropriate gelation time. Where surface amino density and the mass concentration of PAMAM-NH2 were identical, PAMAM with less generation was prone to gelation. The injectable PAMAM/ODex hydrogels have double-crosslinked structures and a high crosslinking density which lead to their high storage modulus. The adhesive strength of the hydrogels is about 2.4 times of commercial available fibrin glue and these hydrogels are nontoxic to L929 mouse fibroblast cells. The L929 cells can attach easily to the surface of hydrogels and proliferate on them, which demonstrates these novel injectable hydrogels are biocompatible and have potential uses in tissue engineering.


Assuntos
Reagentes para Ligações Cruzadas/química , Dendrímeros/química , Hidrogéis/química , Animais , Materiais Biocompatíveis/química , Adesão Celular , Dextranos/química , Dissulfetos/química , Adesivo Tecidual de Fibrina/química , Camundongos , Oxigênio/química , Reologia , Estresse Mecânico , Engenharia Tecidual/métodos
16.
Biomacromolecules ; 17(2): 622-30, 2016 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-26779667

RESUMO

Prompt and strong reconnection of severed peripheral nerves is crucial to nerve regeneration. The development of biocompatible nerve adhesives that are stronger than commonly used fibrin glue would be extremely beneficial to this field. We designed an in situ forming nerve adhesive hydrogel composed of chitosan and ε-polylysine (PL), which mimics the polysaccharides/protein structure of natural epineurium matrices, thus, enhancing the compatibility with nerves. Michael-type addition between the maleimide and thiol group was employed as a cross-linking reaction to eliminate foreign damage to nerves and to ensure a fast hydrogel formation speed (curing speed). Gelation occurred within 10 s, quick enough to promptly seal the transected nerve. Catechol groups conjugated onto PL molecules were demonstrated to reinforce both the bulk cohesive force of the hydrogel and the interfacial adhesive force between the hydrogel and epineurium. The storage modulus of the hydrogel was elevated to more than 2400 Pa. A superior nerve adhesion property that can tolerate 0.185 N of force (8× higher than fibrin glue) was obtained. After 8 weeks, the morphology of the repaired nerve fiber coapted by our hydrogel was very close to the morphology of normal nerve, and the axon cross ratio of the regenerated nerves coapted using hydrogel (57%) was much higher than employing the suture technique (35%). Thus, the in situ rapid gelling system offers a promising approach to the repair of severed peripheral nerves.


Assuntos
Adesivos/química , Quitosana/química , Hidrogéis/química , Nervos Periféricos/fisiopatologia , Polilisina/química , Anastomose Cirúrgica , Animais , Matriz Extracelular/química , Masculino , Teste de Materiais , Mimetismo Molecular , Regeneração Nervosa , Procedimentos Neurocirúrgicos , Nervos Periféricos/cirurgia , Polimerização , Ratos Sprague-Dawley , Tecidos Suporte/química
17.
Exp Ther Med ; 9(2): 563-568, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25574235

RESUMO

The aim of this study was to investigate the effects of Dendrobium candidum Wall. ex Lindl. (D. candidum) on activated carbon-induced constipation in ICR mice. ICR mice were orally administered D. candidum for 9 days. Body weight, defecation status, gastrointestinal (GI) transit and defecation times, in addition to the levels of motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP) and vasoactive intestinal peptide (VIP) in serum were used to evaluate the preventive effects of D. candidum on constipation. The laxative drug bisacodyl acted as a positive control. The time to the first defecation of a black stool for the normal, control, bisacodyl-treated (100 mg/kg), 200 and 400 mg/kg D. candidum-treated mice was 84, 202, 126, 161 and 142 min, respectively. Following the consumption of 200 and 400 mg/kg D. candidum or bisacodyl (100 mg/kg), the GI transit was reduced to 57.7, 74.6 and 90.2%, respectively, of the transit in normal mice. The serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with D. candidum compared with those in the untreated control mice (P<0.05). These results demonstrate that D. candidum has preventive effects on constipation in mice, and a greater functional activity was observed when a higher concentration was administered.

18.
Oncol Lett ; 9(2): 972-978, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25624917

RESUMO

Larimichthys crocea swim bladder is a traditional food and medicine widely used in China. The in vitro anticancer effects of polysaccharide of L. crocea swim bladder (PLCSB) in HCT-116 human colon cancer cells was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. At concentrations ranging between 0 and 800 µg/ml PLCSB, cancer cell viability was decreased by PLCSB in a concentration-dependent manner. In particular, 400 µg/ml PLCSB significantly (P<0.05) induced apoptosis, which was demonstrated by 4,6-diamidino-2-phenylindole staining and flow cytometry analysis. To elucidate the mechanisms underlying the anticancer effect of PLCSB in HCT-116 cancer cells, the expression of apoptosis and metastasis-associated genes was analyzed by reverse transcription-polymerase chain reaction and western blot analysis. A total of 400 µg/ml PLCSB significantly induced apoptosis in HCT-116 cells (P<0.05) via the upregulation Bax, p53, p21, apoptotic protease activating factor 1, caspase-3, -8, and -9, as well as Fas and the downregulation of B-cell lymphoma 2 (Bcl-2), Bcl-extra large and Fas ligand (L). The results of this study demonstrated that PLCSB exhibits an anticancer effect on HCT-116 colon cancer cells, in vitro.

19.
J Cancer Res Ther ; 10(3): 651-7, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25313755

RESUMO

BACKGROUND: Insect tea is a particular drink or health product in China and it is also used as Chinese medicine now. Its functional effects need to be proved. MATERIALS AND METHODS: The ICR mice buccal mucosa cancer model was established by injecting the mice with U14 cells and mice was treated with insect tea. Tumor volumes and lymph node metastasis rates were determined. And the buccal mucosa tissues and cancer cervical lymph node were also checked by histology test, real-time polymerase chain reaction, and western blot assays. RESULTS: The tumor volumes for the group treated with insect tea mice was smaller than those from the control mice. The sections of buccal mucosa cancer tissue showed that the canceration of insect tea mice was weaker than control mice. Insect tea significantly induced apoptosis in buccal mucosa tissues by upregulating Bax, caspases, and downregulating Bcl-2. Nuclear factor-κB, inducible nitric oxide synthase, and COX-2 gene, was significantly downregulated by insect tea, demonstrating its anti-inflammatory properties. Insect tea also exerted a great anti-metastasis effect on tissues as demonstrated by decreased expression of Matrix metalloproteinases genes and increased expression of tissue inhibitors of matrix metalloproteinases. CONCLUSION: The highest concentration of 1600 mg/kg oral gavage and 400 mg/mL smear insect tea showed the best anticancer effects. Based on the results, insect tea showed the strong in vivo buccal mucosa cancer preventive effects.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Insetos/química , Mucosa Bucal/efeitos dos fármacos , Neoplasias Bucais/prevenção & controle , Chá/química , Animais , Apoptose/genética , Caspases/metabolismo , Linhagem Celular Tumoral , Quimioprevenção , Ciclo-Oxigenase 2/genética , Ciclo-Oxigenase 2/metabolismo , Modelos Animais de Doenças , Medicamentos de Ervas Chinesas/administração & dosagem , Feminino , Inflamação/genética , Inflamação/metabolismo , Metaloproteinases da Matriz/genética , Metaloproteinases da Matriz/metabolismo , Camundongos , Mucosa Bucal/patologia , Neoplasias Bucais/genética , Neoplasias Bucais/metabolismo , Neoplasias Bucais/patologia , NF-kappa B/genética , NF-kappa B/metabolismo , Metástase Neoplásica , Óxido Nítrico Sintase Tipo II/genética , Óxido Nítrico Sintase Tipo II/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Carga Tumoral , Proteína X Associada a bcl-2/genética , Proteína X Associada a bcl-2/metabolismo
20.
Exp Ther Med ; 8(4): 1318-1324, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25187847

RESUMO

The aim of the present study was to determine the preventive effect of insect tea against reserpine-induced gastric ulcers in ICR mice. A high (800 mg/kg) dose of insect tea reduced the serum levels of the proinflammatory cytokines interleukin (IL)-6, IL-12, tumor necrosis factor (TNF)-α and interferon (IFN)-γ compared with those in mice treated with a low (400 mg/kg) dose and the control mice. The serum levels of somatostatin (SS) and vasoactive intestinal peptide (VIP) in mice treated with insect tea were higher compared with those in the control mice; however, the serum levels of motilin (MOT) and substance P (SP) were lower in mice treated with insect tea than in the control mice. Gastric ulcer inhibitory rate of the insect tea treatment group of mice were much lower compared to the control mice, and the high concentration treated mice were similar to the ranitidine treated mice. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in mice treated with insect tea were higher compared with those in control mice, and similar to those in normal mice and ranitidine-treated mice. The nitric oxide (NO) and maleic dialdehyde (MDA) levels of mice treated with a high concentration of insect tea compared with the normal group were close. Using quantitative polymerase chain reaction (qPCR) assays, the present study revealed that insect tea significantly induced inflammation in the tissues of mice by downregulating the expression of nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB), inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and upregulating the expression of nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor α (IκB-α). These results suggest that insect tea is as effective at preventing gastric ulcers as the gastric ulcer drug, ranitidine and it can be used as medicine.

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