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1.
Cell Mol Immunol ; 2022 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-35637281

RESUMO

Long noncoding RNAs (lncRNAs) have been implicated in the pathogenesis of intracellular pathogens. However, the role and mechanism of the important lncRNAs in Mycobacterium tuberculosis (M.tb) infection remain largely unexplored. Recently, we found that a secreted M.tb Rv1579c (an early secreted target with a molecular weight of 12 kDa, named EST12) protein activates NLRP3-gasdermin D (GSDMD)-mediated pyroptosis and plays a pivotal role in M.tb-induced immunity. In the present study, M.tb and the EST12 protein negatively regulated the expression of a key lncRNA (named lnc-EST12) in mouse macrophages by activating the JAK2-STAT5a signaling pathway. Lnc-EST12, with a size of 1583 bp, is mainly expressed in immune-related organs (liver, lung and spleen). Lnc-EST12 not only reduces the expression of the proinflammatory cytokines IL-1ß, IL-6, and CCL5/8 but also suppresses the NLRP3 inflammasome and GSDMD pyroptosis-IL-1ß immune pathway through its interaction with the transcription factor far upstream element-binding protein 3 (FUBP3). The KH3 and KH4 domains of FUBP3 are the critical sites for binding to lnc-EST12. Deficiency of mouse lnc-EST12 or FUBP3 in macrophages increased M.tb clearance and inflammation in mouse macrophages or mice. In conclusion, we report a new immunoregulatory mechanism in which mouse lnc-EST12 negatively regulates anti-M.tb innate immunity through FUBP3.

2.
Small ; 18(24): e2201694, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35578914

RESUMO

Inverted perovskite solar cells (PSCs) have received widespread attention due to their facile fabrication and wide applications. However, their power conversion efficiency (PCE) is reported lower than that of regular PSCs because of the undesirable interfacial contact between perovskite and the hydrophobic hole transport layer (HTL). Here, an interface regulation strategy is proposed to overcome this limitation. A small molecule ([2-(9H-carbazol-9-yl) ethyl] phosphonic acid, abbreviated as 2P), composed of carbazole and phosphonic acid groups, is inserted between perovskite and HTL. Morphological characterization and theoretical calculation reveal that perovskite bonds stronger on 2P-modified HTL than on pristine HTL. The improved interfacial contact facilitates hole extraction and retards degradation. Upon the incorporation of 2P, inverted PSCs deliver a high PCE of over 22% with superior stability, keeping 84.6% of initial efficiency after 7200 h storage under an ambient atmosphere with a relative humidity of ≈30-40%. This strategy provides a simple and efficient way to boost the performance of inverted PSCs.

3.
Int J Mol Sci ; 23(9)2022 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-35563641

RESUMO

PIEZO1 is a mechano-sensitive ion channel that can sense various forms of mechanical stimuli and convert them into biological signals, affecting bone-related diseases. The present study aimed to identify key genes and signaling pathways in Piezo1-regulated bone-related diseases and to explain the potential mechanisms using bioinformatic analysis. The differentially expressed genes (DEGs) in tendon, femur, and humerus bone tissue; cortical bone; and bone-marrow-derived macrophages were identified with the criteria of |log2FC| > 1 and adjusted p-value < 0.05 analysis based on a dataset from GSE169261, GSE139121, GSE135282, and GSE133069, respectively, and visualized in a volcano plot. Venn diagram analyses were performed to identify the overlapping DEGs expressed in the above-mentioned tissues. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, protein-protein interaction (PPI) analysis, and module analysis were also conducted. Furthermore, qRT-PCR was performed to validate the above results using primary chondrocytes. As a result, a total of 222 overlapping DEGs and 12 mostly overlapping DEGs were identified. Key Piezo1-related genes, such as Lcn2, Dkk3, Obscn, and Tnnt1, were identified, and pathways, such as Wnt/ß-catenin and PI3k-Akt, were also identified. The present informatic study provides insight, for the first time, into the potential therapeutic targets of Piezo1-regulated bone-related diseases.


Assuntos
Biologia Computacional , Perfilação da Expressão Gênica , Biologia Computacional/métodos , Perfilação da Expressão Gênica/métodos , Ontologia Genética , Fosfatidilinositol 3-Quinases/genética , Mapas de Interação de Proteínas/genética
4.
ACS Nano ; 2022 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-35622531

RESUMO

Pb-free perovskite material is considered to be a promising material utilized in next-generation X-ray detectors due to its high X-ray absorption coefficient, decent carrier transport properties, and relatively low toxicity. However, the pixelation of the perovskite material with an industry-level photolithography processing method remains challenging due to its poor structural stability. Herein, we use Cs2AgBiBr6 perovskite material as the prototype and investigate its interaction with photolithographic polar solvents. Inspired by that, we propose a wafer-scale photolithography patterning method, where the pixeled perovskite array devices for X-ray detection are successfully prepared. The devices based on pixeled Pb-free perovskite material show a high detection sensitivity up to 19118 ± 763 µC Gyair-1 cm-2, which is comparable to devices with Pb-based perovskite materials and superior to the detection sensitivity (∼20 µC Gyair-1 cm-2) of the commercial a-Se detector. After pixelation, the devices achieve an improved spatial resolution capacity with the spatial frequency from 2.7 to 7.8 lp mm-1 at modulation-transfer-function (MTF) = 0.2. Thus, this work may contribute to the development of high-performance array X-ray detectors based on Cs2AgBiBr6 perovskite material.

5.
Front Genet ; 13: 853907, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35464846

RESUMO

Background: Musculocontractural Ehlers-Danlos syndrome (mcEDS) is a rare heritable connective tissue disease with various symptoms. The diagnosis of mcEDS is difficult because of the large overlap of clinical symptoms between different EDS subtypes. Methods: We performed karyotype analysis, gene copy number variation detection, whole-exome sequencing, and Sanger sequencing to reveal the underlying genetic etiology of a fetus with structural abnormalities in feet and kidneys. Results: A likely pathogenic mutation [NM_130468.3 c.958C>T (p.Arg320*)] and an uncertain significance mutation [NM_130468.3 c.896A>G (p.Tyr299Cys)] were identified in the carbohydrate sulfotransferase 14 (CHST14) gene by whole-exome sequencing and validated by Sanger sequencing. Conclusion: The two identified mutations appear highly likely to be the genetic causes of the fetal structural abnormalities.

6.
Biomed Res Int ; 2022: 1234612, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35445130

RESUMO

The aerial parts of Bupleurum Chinense DC. aromatic oil (BAO) were a well-known Chinese herbal medicine plant extract used to treat epilepsy. This study aimed to explore the therapeutic effect of BAO on kainic acid- (KA-) induced epileptic rats and the possible mechanism of its antiepileptic effect. The composition and content of BAO were analyzed by GC-MS, and BAO was administered orally to alleviate the epileptic behavior induced by KA brain injection. The behavior of epileptic rats was determined by Racine grading criteria. And hematoxylin-eosin staining (HE), Nissl staining, immunohistochemistry, Elisa, Western blot, and other methods were used to study the antiepileptic mechanism of BAO, and the possible mechanism was verified by the epileptic cell model of hippocampal neurons induced by the low-Mg2+ extracellular fluid. BAO was mainly composed of terpenoids and aliphatic compounds. And BAO could improve KA-induced epilepsy-like behavior, neuroinflammation, and neurotransmitter abnormalities in the hippocampus. Furthermore, BAO could regulate the expression of GABA, NMDAR1, Notch1, and MAP2 to improve the symptoms of epilepsy. These results were also validated at the cellular level. These results indicated that BAO could alleviate the epilepsy-like behavior through the action of the Notch/NMDAR/GABA pathway.


Assuntos
Bupleurum , Epilepsia , Animais , Anticonvulsivantes/farmacologia , Anticonvulsivantes/uso terapêutico , Epilepsia/induzido quimicamente , Epilepsia/tratamento farmacológico , Epilepsia/metabolismo , Hipocampo/metabolismo , Ácido Caínico/farmacologia , Componentes Aéreos da Planta , Ratos , Ácido gama-Aminobutírico/metabolismo
7.
Oxid Med Cell Longev ; 2022: 7907814, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35432728

RESUMO

Bupleurum chinense DC. is a traditional Chinese medicine with a long medicinal history and is often used as the main ingredient in prescription drugs for epilepsy. The aerial parts of B. chinense DC. have similar efficacy and composition to B. chinense DC. Therefore, we speculated that the aerial parts of B. chinense DC. could be used in the treatment of epilepsy. Polysaccharides from the aerial parts of B. chinense DC. were selected to explore their therapeutic effects on epilepsy and their potential mechanism of action. The study is aimed at clarifying the antiepileptic effects of the polysaccharides from the aerial parts of B. chinense DC. and their potential underlying mechanisms. The chemical profile of the aerial parts of B. chinense DC. polysaccharides (ABP) was characterized by FT-IR spectrum and HPLC chromatogram. To determine the therapeutic effects of ABPs on epilepsy, we established a kainic acid- (KA-) induced rat model of epilepsy, and through H&E staining, Nissl staining, immunohistochemistry, biochemical analysis, ELISA, and Western blot analysis, we explored the mechanisms underlying the therapeutic effects of ABPs on epilepsy. The monosaccharide content of ABP included galacturonic acid (45.19%), galactose (36.63%), arabinose rhamnose (12.13%), and mannose (6.05%). Moreover, the average molecular weight of ABP was 1.38 × 103 kDa. ABP could improve hippocampal injuries and neuronal function in the KA-induced epilepsy rat model. ABP significantly inhibited oxidative stress in the hippocampus of KA-induced rats. More importantly, ABP could regulate TREM2 activation in the PI3K/Akt/GSK-3ß pathway to inhibit neuronal apoptosis, including increasing the expression of superoxide dismutase and lactate dehydrogenase and decreasing the expression of malondialdehyde. The current study defined the potential role of ABP in inhibiting the development of epilepsy, indicating that ABP could upregulate TREM2 to alleviate neuronal apoptosis, by activating the PI3K/Akt/GSK-3ß pathway and oxidative stress in epilepsy.


Assuntos
Bupleurum , Epilepsia , Animais , Bupleurum/química , Bupleurum/metabolismo , Epilepsia/tratamento farmacológico , Glicogênio Sintase Quinase 3 beta/metabolismo , Estresse Oxidativo , Fosfatidilinositol 3-Quinases/metabolismo , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , Polissacarídeos/farmacologia , Polissacarídeos/uso terapêutico , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Transdução de Sinais , Espectroscopia de Infravermelho com Transformada de Fourier
8.
ACS Appl Mater Interfaces ; 14(18): 21287-21294, 2022 May 11.
Artigo em Inglês | MEDLINE | ID: mdl-35484865

RESUMO

Three nonfused ring electron acceptors, Hexyl-0F, Isopropyl-0F, and Isopropyl-2F, are designed and synthesized. Unlike Hexyl-0F, Isopropyl-0F with two sterically hindered 2,4,6-triisopropyl-phenyl groups is highly soluble, which provides a good opportunity for solution processability. Compared with Isopropyl-0F, Isopropyl-2F with fluorinated end-groups exhibits red-shifted absorption. Due to these synergistic adjustment, Isopropyl-2F-based devices displayed a high power conversion efficiency of 12.55%, higher than that of Isopropyl-0F (9.49%). The result demonstrates that the introduction of large steric substituents in the π-bridge units and electron-withdrawing end-groups plays a positive role in the construction of high-efficiency nonfused ring electron acceptors.

9.
Plant Sci ; 318: 111233, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35351305

RESUMO

The cytochrome P450 superfamily (CYP450) is one of the largest protein families in plants, and its members play diverse roles in primary and secondary metabolic biosynthesis. In this study, the CYP450 family gene IbCYP82D47 was cloned from the high carotenoid line HVB-3 of sweet potato (Ipomoea batatas). The IbCYP82D47 protein harbored two transmembrane domains and dynamically localized between plastid stroma and membrane. Overexpression of IbCYP82D47 not only increased total carotenoid, lutein, zeaxanthin and violaxanthin contents by 32.2-48.0%, 10.5-13.3%, 40.2-136% and 82.4-106%, respectively, but also increased the number of carotenoid globules in sweet potato storage roots. Furthermore, genes associated with the carotenoid biosynthesis (IbDXS, IbPSY, IbLCYE, IbBCH, IbZEP) were upregulated in transgenic sweet potato. In addition, IbCYP82D47 physically interacts with geranylgeranyl diphosphate synthase 12 (IbGGPPS12). Our findings suggest that IbCYP82D47 increases carotenoid contents by interacting with the carotenoid biosynthesis related protein IbGGPPS12, and influencing the expressions of carotenoid biosynthesis related genes in transgenic sweet potato.


Assuntos
Ipomoea batatas , Carotenoides/metabolismo , Sistema Enzimático do Citocromo P-450/genética , Sistema Enzimático do Citocromo P-450/metabolismo , Genes de Plantas , Ipomoea batatas/genética , Ipomoea batatas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo
10.
Materials (Basel) ; 15(6)2022 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-35329478

RESUMO

Landslide dams are dangerous because the outburst floods produced by dam failures seriously threaten life and property downstream. In this study, a series of physical flume tests were conducted to investigate the breaching process of landslide dams with fine-grained, well graded, and coarse-grained material under different inflow conditions. The effects of dam material and inflow discharge on the breach development, outflow discharge and erosion characteristics were studied. The erosion resistance of materials and lateral collapses were also discussed. Experimental results reveal that the whole breaching process is determined by the water-sediment interaction. For the fine-grained dams, a general constant downstream slope angle is maintained during the breaching process. For the well-graded dams, a step-pool structure is generated due to the scarp erosion. For the coarse-grained dams, they can remain stable under normal circumstances but fail by overtopping in a short duration under the extreme inflow condition. The final breach of the dam with higher fine content or larger inflow discharge is deeper and narrower. In addition, many fluctuations are observed in the changing curve of the erosion rates along the flow direction for the well-graded and coarse-grained dams. The erosion resistance of materials increases along the flow direction, which needs to be further considered in physically based breach models. Furthermore, the lateral collapse is affected by the dam material instead of inflow discharge. The lower fine content causes more lateral collapses with smaller volumes.

11.
J Eukaryot Microbiol ; : e12907, 2022 Mar 21.
Artigo em Inglês | MEDLINE | ID: mdl-35313044

RESUMO

Amitosis is widespread among eukaryotes, but the underlying mechanisms are poorly understood. The polyploid macronucleus (MAC) of unicellular ciliates divides by amitosis, making ciliates a potentially valuable model system to study this process. However, a method to accurately quantify the copy number of MAC chromosomes has not yet been established. Here, we used droplet digital PCR (ddPCR) to quantify the absolute copy number of the MAC chromosomes in Tetrahymena thermophila. We first confirmed that ddPCR is a sensitive and reproducible method to determine accurate chromosome copy numbers at the single-cell level. We then used ddPCR to determine the copy number of different MAC chromosomes by analyzing individual T. thermophila cells in the G1 and the amitotic (AM) phases. The average copy number of MAC chromosomes was 90.9 at G1 phase, approximately half the number at AM phase (189.8). The copy number of each MAC chromosome varied among individual cells in G1 phase and correlated with cell size, suggesting that amitosis accompanied by unequal cytokinesis causes copy number variability. Furthermore, the fact that MAC chromosome copy number is less variable among AM-phase cells suggests that the copy number is standardized by regulating DNA replication. We also demonstrated that copy numbers differ among different MAC chromosomes and that interchromosomal variations in copy number are consistent across individual cells. Our findings demonstrate that ddPCR can be used to model amitosis in T. thermophila and possibly in other ciliates.

12.
F1000Res ; 11: 25, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35265323

RESUMO

Background: Baylor College of Medicine provides a classroom-based implicit bias workshop to all third-year medical students to increase students' awareness of their unconscious bias and develop strategies for reducing health care disparities. The workshop meets our immediate goals and objectives. However, we are unsure if the benefit would be long-term or diminish over time. Methods: To examine the concept retention from the implicit bias classroom workshop, we administered a self-developed seven-item seven-point Likert-scale survey to our medical students at pre-, post-, and one-year post-workshop attendance. Results: The data set was comprised of survey results from two cohorts of our third and fourth-year medical students from 2018 to 2020 and included 289 completed records at three measurement points. The data included: Student Identifiers, Sex, Race/Ethnicity, Student Enrollment Type, Cohort, and three repeated measures results for each of the seven items, which were documented in wide format. The data may be of interest to those who wish to examine how factors including elapsed time, race, and sex may associate with attitudes and understandings of implicit bias following related training, and those interested in analytical methods on longitudinal research in general.


Assuntos
Estudantes de Medicina , Atitude do Pessoal de Saúde , Humanos , Inquéritos e Questionários , Universidades
13.
Front Public Health ; 10: 843862, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35356011

RESUMO

From 2013 to 2017, progress has been made by implementing the Air Pollution Prevention and Control Action Plan. Under the background of the 3 Year Action Plan to Fight Air Pollution (2018-2020), the pollution status of PM2.5, a typical air pollutant, has been the focus of continuous attention. The spatiotemporal specificity of PM2.5 pollution in the Chinese urban atmospheric environment from 2018 to 2020 can be summarized to help conclude and evaluate the phased results of the battle against air pollution, and further, contemplate the governance measures during the period of the 14th Five-Year Plan (2021-2025). Based on PM2.5 data from 2018 to 2020 and taking 366 cities across China as research objects, this study found that PM2.5 pollution has improved year by year from 2018 to 2020, and that the heavily polluted areas were southwest Xinjiang and North China. The number of cities with a PM2.5 concentration in the range of 25-35 µg/m3 increased from 34 in 2018 to 86 in 2019 and 99 in 2020. Moreover, the spatial variation of the PM2.5 gravity center was not significant. Concretely, PM2.5 pollution in 2018 was more serious in the first and fourth quarters, and the shift of the pollution's gravity center from the first quarter to the fourth quarter was small. Global autocorrelation indicated that the space was positively correlated and had strong spatial aggregation. Local Moran's I and Local Geti's G were applied to identify hotspots with a high degree of aggregation. Integrating national population density, hotspots were classified into four areas: the Beijing-Tianjin-Hebei region, the Fenwei Plain, the Yangtze River Delta, and the surrounding areas were selected as the key hotspots for further geographic weighted regression analysis in 2018. The influence degree of each factor on the average annual PM2.5 concentration declined in the following order: (1) the proportion of secondary industry in the GDP, (2) the ownership of civilian vehicles, (3) the annual grain planting area, (4) the annual average population, (5) the urban construction land area, (6) the green space area, and (7) the per capita GDP. Finally, combined with the spatiotemporal distribution of PM2.5, specific suggestions were provided for the classified key hotspots (Areas A, B, and C), to provide preliminary ideas and countermeasures for PM2.5 control in deep-water areas in the 14th Five-Year Plan.


Assuntos
Monitoramento Ambiental , Material Particulado , Fatores Socioeconômicos , China/epidemiologia , Cidades , Monitoramento Ambiental/métodos , Humanos , Material Particulado/análise , Políticas , Análise Espaço-Temporal
14.
Food Funct ; 13(4): 1774-1784, 2022 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-35112684

RESUMO

Antibiotic-resistant strains of Streptococcus uberis (S. uberis) frequently cause clinical mastitis in dairy cows resulting in enormous economic losses. The regulation of immunometabolism is a promising strategy for controlling this bacterial infection. To investigate whether taurine alleviates S. uberis infection by the regulation of host glycolysis via HIF1α, the murine mammary epithelial cell line (EpH4-Ev) and C57BL/6J mice were challenged with S. uberis. Our data indicate that HIF1α-driven glycolysis promotes inflammation and damage in response to the S. uberis challenge. The activation of HIF1α is dependent on mTOR-mediated ROS production. These results were confirmed in vivo. Taurine, an intracellular metabolite present in most animal tissues, has been shown to effectively modulate HIF1α-triggered metabolic reprogramming and contributes to a reduction of inflammation, which reduces mammary tissue damage and prevents mammary gland dysfunction in S. uberis-induced mastitis. These data provide a novel putative prophylactic and therapeutic strategy for amelioration of dairy cow mastitis and bacterial inflammation.


Assuntos
Glicólise/efeitos dos fármacos , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Infecções Estreptocócicas/metabolismo , Taurina/farmacologia , Animais , Linhagem Celular , Feminino , Glândulas Mamárias Animais/citologia , Camundongos , Camundongos Endogâmicos C57BL , Transdução de Sinais/efeitos dos fármacos , Streptococcus/efeitos dos fármacos
15.
Korean J Physiol Pharmacol ; 26(2): 95-111, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-35203060

RESUMO

Chronic obstructive pulmonary disease (COPD) is an important healthcare problem worldwide. Often, glucocorticoid (GC) resistance develops during COPD treatment. As a classic hypoglycemic drug, metformin (MET) can be used as a treatment strategy for COPD due to its anti-inflammatory and antioxidant effects, but its specific mechanism of action is not known. We aimed to clarify the role of MET on COPD and cigarette smoke extract (CSE)-induced GC resistance. Through establishment of a COPD model in rats, we found that MET could improve lung function, reduce pathological injury, as well as reduce the level of inflammation and oxidative stress in COPD, and upregulate expression of nuclear factor E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), multidrug resistance protein 1 (MRP1), and histone deacetylase 2 (HDAC2). By establishing a model of GC resistance in human bronchial epithelial cells stimulated by CSE, we found that MET reduced secretion of interleukin-8, and could upregulate expression of Nrf2, HO-1, MRP1, and HDAC2. MET could also increase the inhibition of MRP1 efflux by MK571 significantly, and increase expression of HDAC2 mRNA and protein. In conclusion, MET may upregulate MRP1 expression by activating the Nrf2/HO-1 signaling pathway, and then regulate expression of HDAC2 protein to reduce GC resistance.

17.
J Am Chem Soc ; 144(4): 1910-1920, 2022 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-35060705

RESUMO

Deciphering the atomic and electronic structures of interfaces is key to developing state-of-the-art perovskite semiconductors. However, conventional characterization techniques have limited previous studies mainly to grain-boundary interfaces, whereas the intragrain-interface microstructures and their electronic properties have been much less revealed. Herein using scanning transmission electron microscopy, we resolved the atomic-scale structural information on three prototypical intragrain interfaces, unraveling intriguing features clearly different from those from previous observations based on standalone films or nanomaterial samples. These intragrain interfaces include composition boundaries formed by heterogeneous ion distribution, stacking faults resulted from wrongly stacked crystal planes, and symmetrical twinning boundaries. The atomic-scale imaging of these intragrain interfaces enables us to build unequivocal models for the ab initio calculation of electronic properties. Our results suggest that these structure interfaces are generally electronically benign, whereas their dynamic interaction with point defects can still evoke detrimental effects. This work paves the way toward a more complete fundamental understanding of the microscopic structure-property-performance relationship in metal halide perovskites.

18.
Med Teach ; : 1-8, 2022 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-35021935

RESUMO

PURPOSE: Training in implicit bias is broadly recognized as important in medical education and is mandated by some accrediting bodies. This study examined medical students' retention of concepts immediately following and one-year post participation in an implicit bias workshop. METHODS: Study subjects were 272 third-year medical students who participated in workshops held between 2018-2020 that used the Implicit Associations Test (IAT) as a trigger for discussions in small groups. We developed a survey and administered it to students to capture their awareness of implicit bias pre-, post-, and one-year post-workshop attendance. Repeated Measures Analyses and independent-samples t-tests were used to examine for differences in responses on each of the seven survey items and a tabulated 7-item average of these seven items. RESULTS: Six of seven survey items and the tabulated 7-item average examined by Repeated Measures Analyses showed statistically significant increases between the pre-, post-, and one-year post-surveys (ps range: 0.01-0.07), with a small to moderate effect sizes (ƞp2s range: 0.01-0.07). Pairwise comparisons among these three surveys' results indicated statistically significant improvements between the pre- and the post-workshop surveys (ps range: 0.01-0.03) but no statistically significant differences between the post- and the one-year post-workshop surveys (ps range: 0.57-0.99). A separate sample of 17 off-cycle students who took the one-year post- workshop survey two years after the workshop did not differ statistically on the level of awareness of bias compared to those taking the same survey one year later, as examined by the two-group independent t-tests for the seven one-year post-workshop survey items (ps range: 0.56-0.99). CONCLUSIONS: The findings support one-year retention of knowledge and attitudes gained from an implicit bias workshop and suggest similar retention at two years. Future educational interventions that train learners to recognize and manage implicit and explicit behaviors in clinical practice are needed.

19.
BMC Pregnancy Childbirth ; 22(1): 19, 2022 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-34996368

RESUMO

BACKGROUND: Subacute thyroiditis (SAT) is rarely diagnosed in pregnant women, and only 7 cases have been reported to date. Thyroid dysfunction, especially hyperthyroidism, during pregnancy has been associated with both maternal and neonatal complications. Thus, the early diagnosis and treatment of SAT during pregnancy may be beneficial. We present a case report and literature review to complement the diagnostic evaluation and management of SAT during pregnancy. CASE PRESENTATION: A 27-year-old woman presented in gestational week 17 of her first pregnancy and had a negative prior medical history. She presented to the Endocrinology Department complaining of neck pain for one month that had intensified in the last five days. Physical examination revealed a diffusely enlarged thyroid gland that was firm and tender on palpation. The patient also had an elevated temperature and heart rate. The increasing and long-lasting pain coupled with a decreased level of thyroid-stimulating hormone indicated hyperthyroidism. Ultrasound findings were indicative of SAT. Importantly, the pain was so severe that 10 mg of oral prednisone per day was administered in gestational week 18, which was increased to 15 mg/d after 10 days that was discontinued in week 28. Levothyroxine was started in gestational week 24 and administered throughout the pregnancy. The patient responded well to the treatments, and her neck pain disappeared in gestational week 21. She gave birth to a healthy male in gestational week 41. CONCLUSION: SAT can be diagnosed and effectively managed during pregnancy, thus benefiting mothers and infants.


Assuntos
Complicações na Gravidez/diagnóstico , Tireoidite Subaguda/diagnóstico , Adulto , Feminino , Glucocorticoides/uso terapêutico , Humanos , Nascido Vivo , Cervicalgia/etiologia , Prednisona/uso terapêutico , Gravidez , Complicações na Gravidez/tratamento farmacológico , Resultado da Gravidez , Testes de Função Tireóidea , Tireoidite Subaguda/tratamento farmacológico , Tiroxina/uso terapêutico , Resultado do Tratamento
20.
ACS Appl Mater Interfaces ; 14(4): 6039-6047, 2022 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-35061346

RESUMO

Nonfused-ring electron acceptors have attracted much attention in recent years due to their advantages of simple synthetic routes, high yields, low costs, reasonable power conversion efficiencies (PCEs), and so on. Herein, three simple A-π-D-π-A-type acceptors (DTC-BO-4F, DTS-BO-4F, and DTP-BO-4F) comprising a tricyclic fused-ring core, two 2,5-bis(alkyloxy)phenylene spacers, and two difluorinated terminal groups (DF-IC) were developed. Compared with DTS-BO-4F, DTC-BO-4F and DTP-BO-4F exhibit higher molar extinction coefficients, stronger crystallinity, and more orderly stacking. The PBDB-T:DTC-BO-4F-based blend film shows suitable phase separation and higher and more balanced charge mobilities. Finally, the photovoltaic devices based on DTC-BO-4F give an outstanding PCE of 13.26% with a small nonradiative voltage loss of 0.23 eV.

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