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1.
Biomark Med ; 13(15): 1255-1261, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31580146

RESUMO

Aim: The prognostic role of neutrophil-to-lymphocyte ratio (NLR) in bloodstream infection (BSI) deserves further investigation. Patients & methods: The NLR values were measured and compared in BSI patients and healthy controls. The receiver operating characteristic of NLR and cut-off values were measured in BSI patients and subgroups. Results: We have measured the NLR of study group with 2160 BSI patients and normal group with 2523 healthy controls, which was significantly high in study group (11.36 ± 21.38 vs 2.53 ± 0.86; p < 0.001) and the area under the curve was 0.834 (95% CI: 0.825-0.842; p < 0.001). The critical value of NLR for diagnosis of BSI was 3.09, with a sensitivity of 75.3%, and a specificity of 93.6%. Conclusion: NLR is an effective diagnostic indicator of including BSIs of Gram-negative bacteria, Gram-positive bacteria and fungus.

2.
Hepatobiliary Pancreat Dis Int ; 18(5): 403-411, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31303562

RESUMO

BACKGROUND: Acute-on-chronic liver failure (ACLF) refers to the acute deterioration of liver function that occurs in patients with chronic liver disease. ACLF is characterized by acute decompensation, organ failure and high short-term mortality. Numerous studies have been conducted and remarkable progress has been made regarding the pathophysiology and pathogenesis of this disease in the last decade. The present review was to summarize the advances in this field. DATA SOURCES: A comprehensive search in PubMed and EMBASE was conducted using the medical subject words "acute-on-chronic liver failure", "ACLF", "pathogenesis", "predictors", and "immunotherapy" combined with free text terms such as "systemic inflammation" and "immune paralysis". Relevant papers published before October 31, 2018, were included. RESULTS: ACLF has two marked pathophysiological features, namely, excessive systemic inflammation and susceptibility to infection. The systemic inflammation is mainly manifested by a significant increase in the levels of plasma pro-inflammatory factors, leukocyte count and C-reactive protein. The underlying mechanisms are unclear and may be associated with decreased immune inhibitory cells, abnormal expression of cell surface molecules and intracellular regulatory pathways in immune cells and increased damage-associated molecular patterns in circulation. However, the main cause of susceptibility to infection is immune paralysis. Immunological paralysis is characterized by an attenuated activity of immune cells. The mechanisms are related to elevations of immune inhibitory cells and the concentration of plasma anti-inflammatory molecules. Some immune biological indicators, such as soluble CD163, are used to explore the pathogenesis and prognosis of the disease, and some immunotherapies, such as glucocorticoids and granulocyte colony-stimulating factor, are effective on ACLF. CONCLUSIONS: Overwhelming systemic inflammation and susceptibility to infection are two key features of ACLF. A better understanding of the state of a patient's immune system will help to guide immunotherapy for ACLF.

3.
Int J Clin Exp Pathol ; 8(7): 8419-25, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26339412

RESUMO

OBJECTIVE: To investigate the diagnostic values of soluble cluster of differentiation 163 (sCD163) in patients with liver failure or various inflammations. METHODS: Serum samples were collected from patients admitted to the First Affiliated Hospital, Zhejiang University from October 2013 to January 2015 for treatment of with liver diseases, including liver failure (n=38), hepatitis B virus (HBV)-induced liver cancer (HBsAg positive) (n=40), HBV-induced hepatic cirrhosis (HBsAg positive) (n=40), chronic hepatitis B (n=38), HBV carrier (n=40), fatty liver patients without HBV infection (n=40), chronic glomerulonephritis (n=38), community acquired pneumonia (n=38) and acute pancreatitis (n=38). The CD163/sCD163 was determined using commercial ELISA kits according to the manufacturer's instructions. RESULTS: Significant decrease was noticed in the sCD163 in patients with fatty liver and HBV carrier compared with that of patients with chronic hepatitis B (P < 0.05). Compared with the healthy controls, the level of sCD163 was remarkably increased in the other groups (P < 0.05). The serum sCD163 in patients with HBV-induced liver cancer showed statistical difference compared with those of the patients with fatty liver, HBV carrier, as well as those with liver failure (P < 0.05). The expression of sCD163 was remarkably elevated in patients with liver failure compared with the patients with liver cancer, HBV-induced hepatic cirrhosis, chronic hepatitis B, fatty liver, or HBV carrier (P < 0.05). No significant difference was noticed in the sCD163 in patients with chronic hepatitis B, community acquired pneumonia, chronic glomerulonephritis, and acute pancreatitis (P > 0.05). CONCLUSIONS: sCD163 is a sensitive marker protein for liver failure. The elevation of sCD163 was closely related to the progression of the liver failure. No statistical difference was noticed in the sCD163 in patients with inflammatory disorders, indicating sCD163 showed no organ specificity.


Assuntos
Antígenos CD/sangue , Antígenos de Diferenciação Mielomonocítica/sangue , Inflamação/sangue , Falência Hepática/sangue , Receptores de Superfície Celular/sangue , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , China , Ensaio de Imunoadsorção Enzimática , Feminino , Hospitais Universitários , Humanos , Inflamação/diagnóstico , Falência Hepática/diagnóstico , Falência Hepática/etiologia , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Fatores de Risco , Regulação para Cima
4.
Int J Clin Exp Pathol ; 8(4): 4099-105, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26097598

RESUMO

BACKGROUND: Several cytokines have been involved in the diagnosis and prognosis for the pathogenesis and severity of chronic hepatitis B (CHB) such as cluster of differentiation 163 (CD163), neutrophil gelatinase-associated lipocalin (NGAL), high-mobility group box 1 (HMGB1) and macrophage inflammatory protein-2 (MIP-2). Nevertheless, the stability and reliability of these cytokines can be greatly influenced by handling and storage processes. METHODS: In this study, potential utility of serum samples of a CHB cohort was evaluated to investigate several processes that might impact cytokine profiles such as temperature, storage time and number of freeze-thaw cycles. Blood samples collected from 100 patients with CHB were separated immediately and divided into two groups. In one group, samples (n=50) stored at -80 °C were subject to 1-3 freeze-thaw cycles. In the other group, samples (n=50) were stored at 4 °C and 25 °C for 3 h, 9 h, 24 h, 48 h, 72 h, and 7 d time points, respectively. To assess the influence of different storage conditions on cytokines, the levels of CD163, NGAL, HMGB1 and MIP-2 were measured using enzyme-linked immunosorbent assays (ELISA) kits. RESULTS: No significant differences of these four cytokines after 1-3 repeated freeze-thaw cycles. Significant differences of NAGL levels were seen between 9 h and 7 d (P<0.05), and also in HMGB1 at 25 °C, while the other cytokines were relatively stable at the two storage temperatures over the various time points. CONCLUSION: This study indicated that these four cytokines remained stable within three freeze-thaw cycles and 7 d at 4 °C. No perceptible effects on CD163 and MIP-2 levels were presented under the storage condition of 7 d at room temperature, whereas the degradation of NGAL and HMGB1 were notable.


Assuntos
Antígenos CD/sangue , Antígenos de Diferenciação Mielomonocítica/sangue , Quimiocina CXCL2/sangue , Proteína HMGB1/sangue , Lipocalinas/sangue , Proteínas Proto-Oncogênicas/sangue , Receptores de Superfície Celular/sangue , Manejo de Espécimes/métodos , Proteínas da Fase Aguda , Adolescente , Adulto , Idoso , Biomarcadores/sangue , Criança , Pré-Escolar , Temperatura Baixa , Ensaio de Imunoadsorção Enzimática , Feminino , Congelamento , Humanos , Lipocalina-2 , Masculino , Pessoa de Meia-Idade , Desnaturação Proteica , Estabilidade Proteica , Fatores de Tempo , Adulto Jovem
5.
Cell Physiol Biochem ; 33(6): 1933-44, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25034766

RESUMO

BACKGROUND: Fulminant hepatitis is a severe liver disease characterized by massive hepatocyte necrosis and clinical signs of liver failure. This study explores the expression profile of microRNAs, which are regulators of a number of pathophysiological processes, during the early stage of concanavalin A (Con A)-induced hepatitis. METHODS: Balb/c mice were given ConA injections to induce fulminant hepatitis. miRNA expression profiling in liver tissues was carried out by microarray analysis. The differentially expressed miRNAs were subjected to time sequence profile analysis, gene-miRNA regulatory network analysis, and gene ontology-miRNA regulatory network analysis. RESULTS: Eleven miRNAs among multiClass were found to be significantly differentially expressed between liver tissue in early stage fulminant hepatitis and normal control liver tissue. Mmu-miR-133a was the most differentially expressed with the strongest regulatory ability, regulating 47 mRNAs. Mmu-miR-10a was the most highly expressed in the microRNA-GO-Network and also exerted a strong regulatory ability. The expression profiles of miR-133a and miR-10a were verified by RT-PCR. CONCLUSIONS: These results show that, in the early stage, ConA-induced fulminant hepatitis induces a distinct miRNA expression profile. This differential miRNA expression profile may provide pathogenic clues and potential diagnostic and prognostic markers in acute and severe liver disease.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/genética , Fígado/metabolismo , MicroRNAs/genética , Transcriptoma , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Bilirrubina/sangue , Doença Hepática Induzida por Substâncias e Drogas/sangue , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Concanavalina A , Ontologia Genética , Redes Reguladoras de Genes , L-Lactato Desidrogenase/sangue , Fígado/patologia , Masculino , Camundongos Endogâmicos BALB C , Modelos Genéticos , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase Via Transcriptase Reversa
6.
World J Gastroenterol ; 20(23): 7505-13, 2014 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-24966622

RESUMO

AIM: To investigate the association of hypertension and diabetes mellitus (DM) with interferon-associated retinopathy (IAR) risk in chronic hepatitis C (CHC). METHODS: Two investigators independently searched PubMed and Embase for eligible articles published prior to December 2013; additional studies were identified by reviewing the bibliographies. Only case-control or cohort studies that evaluated the association between hypertension and/or DM and IAR incidence in CHC patients were included. IAR was characterized by the presence of cotton-wool spots and/or retinal hemorrhage, and was defined as the primary efficacy measure. Pooled relative risks (RRs) with 95% confidence intervals (CIs) were estimated using data extracted from papers based on random-effects models. RESULTS: Eight eligible studies were included in the present meta-analysis. The outcomes showed that patients with CHC and hypertension were at higher risk of IAR (48/189 vs 96/455, RR = 1.90; 95%CI: 1.15-3.15, P < 0.05). Patients with DM receiving interferon (IFN)-based therapy for CHC infection may be at higher risk for IAR (18/72 vs 60/256, RR = 1.56, 95%CI: 1.11-2.20, P < 0.05); however, the outcome was not stable. There was no significant difference in IAR risk between genotype-1-infected patients and non-genotype-1-infected patients (RR = 1.09, 95%CI: 0.64-1.87, P > 0.05). Comparable incidences of IAR were also found between patients treated with pegylated interferon (PIFN) α-2a and those treated with PIFN α-2b (RR = 0.84, 95%CI: 0.56-1.24, P > 0.05) and between patients treated with IFN α and those treated with PIFN α (RR = 1.04, 95%CI: 0.72-1.50, P > 0.05). CONCLUSION: Patients with hypertension have a higher risk of retinopathy when receiving IFN-based therapy for CHC.


Assuntos
Antivirais/efeitos adversos , Diabetes Mellitus/epidemiologia , Hepatite C Crônica/tratamento farmacológico , Hipertensão/epidemiologia , Interferons/efeitos adversos , Doenças Retinianas/induzido quimicamente , Diabetes Mellitus/diagnóstico , Retinopatia Diabética/epidemiologia , Hepatite C Crônica/diagnóstico , Hepatite C Crônica/epidemiologia , Humanos , Hipertensão/diagnóstico , Retinopatia Hipertensiva/epidemiologia , Incidência , Doenças Retinianas/epidemiologia , Medição de Risco , Fatores de Risco
7.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 40(6): 588-92, 652, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22190517

RESUMO

OBJECTIVE: To investigate the influence of miR-122 on IFN-α treatment for HCV infection. METHODS: Huh7.5.1 cells infected with HCV were treated with miR-122 mimics (20 nmol/L, 100 nmol/L, 400 nmol/L) and/or IFN-α (1000 IU/ml). The relative expression of HCV RNA was detected by real-time polymerase chain reaction (PCR). Huh7.5.1 cells were treated with different amounts of HCV (107 copies, 106 copies and 105 copies) and/or IFN-α (1000 IU/ml). RESULTS: IFN-α suppressed the replication of HCV in a time-dependent manner, resulting in a ≊ 83% reduction of HCV at 48 h. MiR-122 mimics facilitated replication of HCV RNA in a dose-dependent manner (P<0.05). The antiviral effect of IFN-α was inverted to levels of miR-122 mimics (20 nmol/L, 100 nmol/L, 400 nmol/L), (73.3% ± 3.5% compared with 84% ± 4.5%, P>0.05; 64.67% ± 5.5% compared with 84% ± 4.5%, P>0.05; 56.33% ± 5.1% compared with 84% ± 4.5%, P<0.05). The antiviral effect of IFN-α was inverted to HCV load (105 copies group compared with 107 copies group, P<0.05). CONCLUSION: MiR-122 facilitates replication of HCV RNA in the cell culture system; and the expression of miR-122 may partly counteract the anti-HCV effect of IFN-α.


Assuntos
Hepacivirus/efeitos dos fármacos , Interferon-alfa/farmacologia , MicroRNAs/genética , Antivirais/farmacologia , Linhagem Celular Tumoral , Hepacivirus/genética , Hepacivirus/fisiologia , Humanos , RNA Viral/genética , Transfecção , Replicação Viral/efeitos dos fármacos , Replicação Viral/genética
8.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 40(6): 593-7, 2011 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-22190518

RESUMO

OBJECTIVE: To investigate the effect of miR-122 on the expression of hepatitis B virus (HBV) proteins. METHODS: Anti-sense oligodeoxynucleotide (ASODN) of two different sequences against miR-122, anti-miR-122 and LNA-antimiR-122 (Locked nucleic acid), human miR -122 (hsa-miR-122), or the negative control anti-GFP were designed and synthesized, then transfected into HepG2.2.15 cells. After 24 h and 48 h, the levels of HBsAg and HBeAg in the supernatant were determined with a time-resolved immunofluorometric assay (TRFIA). HBV DNA in supernatant and miR-122 in cells were measured by quantitative real-time PCR. RESULTS: After 48 h expressions of miR-122 in the LNA-antimiR-122 and anti-miR-122 groups were significantly suppressed and lower than those in the negative control (P<0.001), while the level of miR-122 in the hsa-miR-122 group was higher than that in the negative control (P<0.001). The expression of HBeAg and HBsAg in hsa-miR-122 group was lower than that in the negative control (P<0.01) 24 h and 48 h after transfection. The expression of HBeAg and HBsAg in the anti-miR-122 group and LNA-antimiR-122 group was significantly lower than that in negative control (P>0.001). The levels of viral DNA at both time-points in the various test groups were not significantly different from those of negative control group (P>0.05). CONCLUSION: miR-122 may regulate HBV antigens and potentially affect the progress of pathogenesis, which might be the new targets for treatment of HBV infection.


Assuntos
Antígenos de Superfície da Hepatite B/metabolismo , Antígenos E da Hepatite B/metabolismo , MicroRNAs/genética , DNA Viral/genética , Células Hep G2 , Vírus da Hepatite B/genética , Humanos , MicroRNAs/metabolismo , Transfecção
9.
Zhonghua Wai Ke Za Zhi ; 48(15): 1137-40, 2010 Aug 01.
Artigo em Chinês | MEDLINE | ID: mdl-21055005

RESUMO

OBJECTIVE: To explore the relationship between systemic inflammatory response syndrome(SIRS) and severity of acute pancreatitis combined with plateau erythrocythemia in the high altitude. METHODS: A retrospective analysis on the clinical data which involved acute pancreatitis combined with plateau erythrocythemia (n = 40) and without plateau erythrocythemia (n = 40) admitted from September 2006 to September 2009 was conducted. According to the unified standards, these cases were divided into plateau erythrocythemia group and no plateau erythrocythemia group. The patients in plateau erythrocythemia group were further divided into severe group and mild group according to scores of APACHEII. The data was analyzed according to the patient with (or without) SIRS, SIRS's standard indicators, diagnostic parameter and relation of severity and duration of SIRS in acute pancreatitis combined with plateau erythrocythemia. RESULTS: There was significantly discrepancy between plateau erythrocythemia group and no plateau erythrocythemia group not only in the incidence of patients who developed SIRS, but also in two items of patients fulfilling or not fulfilling diagnostic criteria of SIRS (P < 0.05). There was significant statistical difference in three items of diagnostic parameter of SIRS between plateau erythrocythemia group and no plateau erythrocythemia group (P < 0.05). Significant difference in two and three diagnostic parameter was found on severity of SIRS in acute pancreatitis combined with plateau erythrocythemia (P < 0.05). The more severity acute pancreatitis combined with plateau erythrocythemia was, the longer duration of SIRS was. CONCLUSION: SIRS is highly correlated with the severity of SIRS in acute pancreatitis combined with plateau erythrocythemia in the high altitude.


Assuntos
Altitude , Pancreatite/complicações , Policitemia/complicações , Síndrome de Resposta Inflamatória Sistêmica/etiologia , APACHE , Doença Aguda , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
10.
Exp Mol Med ; 42(7): 477-83, 2010 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-20530982

RESUMO

The scaffold protein IQGAP1 shows elevated levels in several cancer types, but its expression in hepatocellular carcinoma is unknown. We found that 58% of human hepatocellular carcinoma tissue samples had increased IQGAP1 expression compared to adjacent normal tissue. Overexpressing IQGAP1 raised the in vivo tumorigenicity of hepatocellular carcinoma cells, and forced overexpression of IQGAP1 in vitro stimulated cell proliferation. Cell growth was reduced by knockdown or mutation of IQGAP1, or by treatment of cells with a phosphotidylinositol 3-kinase inhibitor. To determine the mechanism by which IQGAP1 overexpression affected hepatocellular carcinoma cells, we confirmed its interaction in these cells with mammalian target of rapamycin (mTOR), a serine/threonine kinase that integrates signals about nutrient and energy status with downstream effectors that influence cell division. In addition, we discovered a new interaction involving IQGAP1, mTOR and Akt, which is a downstream target of mTOR. Akt phosphorylation on Ser-473, which is catalyzed by mTOR and required for Akt activation, increased with increasing amounts of IQGAP1, and decreased with IQGAP1 mutation. We hypothesize that IQGAP1 is a scaffold that facilitates mTOR and Akt interaction.


Assuntos
Carcinoma Hepatocelular/enzimologia , Carcinoma Hepatocelular/patologia , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/enzimologia , Neoplasias Hepáticas/patologia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Proteínas Ativadoras de ras GTPase/metabolismo , Animais , Proliferação de Células , Ativação Enzimática , Células Hep G2 , Humanos , Camundongos , Fosfatidilinositol 3-Quinases/metabolismo , Ligação Proteica , Serina-Treonina Quinases TOR/metabolismo , Regulação para Cima , Proteínas Ativadoras de ras GTPase/genética
11.
Oncol Rep ; 23(5): 1457-62, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20372864

RESUMO

Dysregulation of the antiapoptotic protein cellular FLICE-like inhibitory protein (c-FLIP) has been proven to be associated with tumorigenesis and progress of most human cancers. However, its aberrant expression is poorly elucidated. MicroRNAs (miRNAs) are small non-coding RNAs that are involved in tumorigenesis through negatively regulating gene expression. Our study disclosed that c-FLIP was overexpressed in HepG2 hepatocellular carcinoma cells and down-regulation of c-FLIP enhanced taxol-induced apoptosis. Taxol induction significantly decreased the protein level of c-FLIP. While no decrease in c-FLIP mRNA level was observed, indicating taxol decreased c-FLIP expression through a post-transcriptional mechanism. miR-512-3p was a predicted suppressor of c-FLIP and exhibited an opposite expression manner to c-FLIP before and after taxol induction. Luciferase report assay demonstrated miR-512-3p negatively regulated c-FLIP expression via a conserved miRNA-binding site in 3' untranslated region (3'UTR) of c-FLIP. The decrease of c-FLIP protein due to transfection of miR-512-3p further validated the inhibitory effect of miR-512-3p on c-FLIP. Additional transfection of miR-512-3p remarkably promoted taxol-induced apoptosis, confirming its involvement in apoptosis. In summary, our study disclosed a novel regulatory mechanism that down-regulation of c-FLIP by miR-512-3p contributed to taxol-induced apoptosis. Importantly, the pivotal role of miR-512-3p in determining c-FLIP abundance helps to broaden the implications for cancer therapy by developing small molecules to directly target c-FLIP at mRNA level.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Proteína Reguladora de Apoptosis Semelhante a CASP8 e FADD/metabolismo , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/patologia , MicroRNAs/metabolismo , Paclitaxel/farmacologia , Regiões 3' não Traduzidas , Apoptose/genética , Sequência de Bases , Sítios de Ligação , Proteína Reguladora de Apoptosis Semelhante a CASP8 e FADD/genética , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/metabolismo , Biologia Computacional , Regulação para Baixo , Regulação Neoplásica da Expressão Gênica , Células Hep G2 , Humanos , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , Dados de Sequência Molecular , Interferência de RNA , RNA Mensageiro/metabolismo , Fatores de Tempo , Transfecção
12.
BMC Genomics ; 11: 240, 2010 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-20398290

RESUMO

BACKGROUND: Due to the high morbidity and mortality of fulminant hepatitis, early diagnosis followed by early effective treatment is the key for prognosis improvement. So far, little is known about the gene expression changes in the early stage of this serious illness. Identification of the genes related to the very early stage of fulminant hepatitis development may provide precise clues for early diagnosis. RESULTS: Balb/C mice were used for ConA injection to induce fulminant hepatitis that was confirmed by pathological and biochemical examination. After a gene chip-based screening, the data of gene expression in the liver, was further dissected by ANOVA analysis, gene expression profiles, gene network construction and real-time RT-PCR. At the very early stage of ConA-triggered fulminant hepatitis, totally 1,473 genes with different expression variations were identified. Among these, 26 genes were finally selected for further investigation. The data from gene network analysis demonstrate that two genes, MPDZ and Acsl1, localized in the core of the network. CONCLUSIONS: At the early stages of fulminant hepatitis, expression of twenty-six genes involved in protein transport, transcription regulation and cell metabolism altered significantly. These genes form a network and have shown strong correlation with fulminant hepatitis development. Our study provides several potential targets for the early diagnosis of fulminant hepatitis.


Assuntos
Perfilação da Expressão Gênica , Hepatite Animal/genética , Falência Hepática Aguda/genética , Algoritmos , Análise de Variância , Animais , Concanavalina A , Hepatite Animal/induzido quimicamente , Falência Hepática Aguda/induzido quimicamente , Camundongos , Camundongos Endogâmicos BALB C , Análise de Sequência com Séries de Oligonucleotídeos
13.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue ; 22(4): 210-3, 2010 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-20398464

RESUMO

OBJECTIVE: To explore characteristics of the pathogenesis and progression of the acute pancreatitis (AP) in high altitude and the relationship between AP and plateau erythrocythemia. METHODS: Retrospective analysis of the clinical data of AP was conducted for 103 inpatients who were admitted during 2003 and 2005 to the People's Hospital of Qinghai Province, including 12 cases of AP complicated with plateau erythrocythemia and 91 cases of AP no complicating plateau erythrocythemia. The patients were divided into a group of 57 cases living in high altitude (>3 000 m) and 46 patients in lower altitude group (<2 200 m). Clinical data of the patients were collected at admission, and liver, kidney and lung functions were determined for all patients. RESULTS: Alanine aminotransferase (ALT) and creatinine (Cr) were significantly higher in AP complicating plateau erythrocythemia compared with AP patients without complicating plateau erythrocythemia [ALT: (160.70 + or - 19.14) U/L vs. (78.00 + or - 14.96) U/L, Cr: (135.45 + or - 11.99) micromol/L vs. (91.42 + or - 17.08) micromol/L, both P<0.05]. Arterial partial pressure of oxygen (PaO(2)) and arterial oxygen saturation (SaO(2)) were significantly lower in AP with complication of plateau erythrocythemia than in AP without complicating plateau erythrocythemia [PaO(2): (45.10 + or - 0.40) mm Hg vs. (65.48 + or - 1.36) mm Hg, 1 mm Hg=0.133 kPa, SaO(2): 0.851 + or - 0.004 vs. 0.940 + or - 0.009, both P<0.05]. There was no difference in aspartate aminotransferase (AST), blood urea nitrogen (BUN) and arterial partial pressure of carbon dioxide (PaCO(2)), however, their levels were higher in plateau erythrocythemia cases than those without plateau erythrocythemia [AST: (87.35 + or - 8.10) U/L vs. (83.00 + or - 18.61) U/L, BUN:(10.90 + or - 0.97) mmol/L vs. (7.37 + or - 0.98) mmol/L, PaCO(2): (33.20 + or - 0.31) mm Hg vs. (25.32 + or - 1.14) mm Hg , all P>0.05]. ALT and Cr were significantly higher in high altitude cases than those in lower altitude cases [ALT: (126.92 + or - 15.46) U/L vs. (86.00 + or - 10.23) U/L, Cr:(126.10 + or - 10.01)micromol/L vs. (101.84 + or - 5.46) micromol/L, both P<0.05]. There was no difference in AST, BUN and PaCO(2), however, the values were slightly higher in high altitude cases compared with lower altitude cases [AST: (98.70 + or - 8.10) U/L vs. (93.14 + or - 21.46) U/L, BUN: (8.15 + or - 1.00) mmol/L vs. (5.86 + or - 0.40) mmol/L, PaCO(2): (32.32 + or - 1.01) mm Hg vs. (30.12 + or - 2.76) mm Hg, all P>0.05]. There was no difference in PaO(2) and SaO(2), however, it was slightly lower in high altitude cases than lower altitude cases [PaO(2): (58.80 + or - 1.20) mm Hg vs. (66.86 + or - 3.20) mm Hg, SaO(2): 0.910 + or - 0.011 vs. 0.930 + or - 0.008, both P>0.05]. CONCLUSION: The results showed that the deterioration of hepatic, kidney and lung function in AP patients living in the plateau was related to high altitude and erythrocythemia.


Assuntos
Altitude , Insuficiência de Múltiplos Órgãos/etiologia , Pancreatite/fisiopatologia , Doença Aguda , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Insuficiência de Múltiplos Órgãos/fisiopatologia , Pancreatite/complicações , Policitemia/complicações , Policitemia/fisiopatologia , Prognóstico , Estudos Retrospectivos , Adulto Jovem
14.
Zhongguo Gu Shang ; 22(10): 787-9, 2009 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-19902768

RESUMO

OBJECTIVE: To introduce the methods of dacron flap application and its indications in treating fracture of multiple ribs, in order to reduce the incidence of the complications of fracture displacement. METHODS: From September 2006 to March 2008, 12 patients with fracture of multiple ribs were treated with absorbable rib fixed nail and dacron flap. Included 8 males and 4 females,the age was from 22 to 51 years with an average of 38.2 years,the operative time was 2 hours to 3 days after injured. All the patients were closed injury and simultaneously accompanied with significant chest pain and chest tightness. 4 cases with dyspnea, blood in sputum and blood oxygen saturation decreased. The X-ray showed 3 cases of unilateral fracture and 9 cases of bilateral rib fractures and all cases accompanied with hemopneumothorax. RESULTS: All patients were followed up from 2 to 26 months with an average of 8 months. All the fractures healed. According to clinical criteria, pain, breathing, ribs alignment etc. to observe the effect, 10 cases got excellent result, 1 case good, 1 case poor. CONCLUSION: It is safe and effective to use absorbable rib fixed nails and dacron flap for treating fracture of multiple ribs and especially for the patients of osteoporosis, comminuted fracture or oblique fracture.


Assuntos
Fraturas das Costelas/cirurgia , Adulto , Pinos Ortopédicos , Feminino , Fixação Intramedular de Fraturas , Humanos , Masculino , Pessoa de Meia-Idade , Retalhos Cirúrgicos , Resultado do Tratamento , Adulto Jovem
15.
Clin Cancer Res ; 14(1): 74-81, 2008 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-18172255

RESUMO

PURPOSE: Prognostic markers discovery is a strategy for early diagnosis and individualization therapy for human cancer. In this study, we focus to integrate different methods to identify specific biomarker and elucidate its clinical significance. EXPERIMENTAL DESIGN: A powerful tool named Digital Gene Expression Display online was applied to isolate differentially expressed genes correlated with gastric cancer. Matrix metalloproteinase 11 (MMP11) was selected and confirmed at both mRNA and protein level in 10 cell lines, 123 cases of tumor tissues, and 305 cases of gastric cancer serum specimen by semiquantitative PCR, immunohistochemistry staining, and ELISA techniques, respectively. RESULTS: Our data showed that overexpression of MMP11 at mRNA and protein level was consistently detected in cell lines and primary tumors compared with matched normal tissues. Importantly, serum MMP11 levels were also significantly elevated in gastric cancer patients compared with those of the control subjects (P < 0.001), and the positive expression was well correlated with metastasis in gastric cancer patients (P = 0.009). Furthermore, we have shown that overexpression of MMP11 was associated with the malignant proliferation of AGS cells. CONCLUSIONS: Combination of gene expression profiling and specific clinical resource is a promising approach to validate gene expression patterns associated with malignant phenotype. As a secreted protein, MMP11 may play an important role in carcinogenesis and has potential implication as a biomarker for the diagnosis and prognosis of human cancers including gastric cancer.


Assuntos
Biomarcadores Tumorais/sangue , Perfilação da Expressão Gênica/métodos , Metaloproteinase 11 da Matriz/sangue , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Western Blotting , Linhagem Celular Tumoral , Bases de Dados Genéticas , Progressão da Doença , Ensaio de Imunoadsorção Enzimática , Etiquetas de Sequências Expressas , Feminino , Expressão Gênica , Biblioteca Gênica , Humanos , Imuno-Histoquímica , Masculino , Metaloproteinase 11 da Matriz/genética , Pessoa de Meia-Idade , Prognóstico , RNA Mensageiro/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias Gástricas/genética , Análise Serial de Tecidos
16.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 36(6): 524-30, 2007 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-18067223

RESUMO

OBJECTIVE: To develop a high-throughput diagnostic method with suspension array technique for detecting pathogenic microbes. METHODS: The probes and positive controls of 56 kinds of pathogenic microbes were designed, synthesized, and used to detect pathogenic microbes with suspension array technique. RESULTS: Fluorescence signals of 56 positive controls were higher than those of the negative controls, and there was no cross-reaction between the probes and positive controls of different microbes. CONCLUSION: Based on suspension array technique, the high-throughput diagnostic method may be useful in clinical detection of pathogenic microbes.


Assuntos
Doenças Transmissíveis/microbiologia , Doenças Transmissíveis/virologia , Técnicas Microbiológicas , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Animais , Enterovirus/isolamento & purificação , Humanos , Vírus da Caxumba/isolamento & purificação , Pseudomonas aeruginosa/isolamento & purificação
17.
J Zhejiang Univ Sci B ; 7(9): 745-8, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16909477

RESUMO

OBJECTIVE: To investigate the viral contamination of invasive medical instruments in dentistry and to provide health administrative institutions with surveillance data. METHODS: Sterilized samples were randomly collected from the department of dentistry to detect HBV-DNA, HCV-RNA, HIV-RNA and HBsAg. RESULTS: Of the invasive medical instruments that were sterilized with 2% glutaraldehyde, one of the samples was positive for HBV-DNA, and another sample was positive for HBsAg. CONCLUSION: Though massive virus contamination of invasive medical instruments in dentistry has been reduced to a low level, the occurrence of contamination still remains.


Assuntos
Instrumentos Odontológicos/virologia , Contaminação de Equipamentos , HIV/isolamento & purificação , Hepacivirus/isolamento & purificação , Vírus da Hepatite B/isolamento & purificação , DNA Viral/análise , Antígenos de Superfície da Hepatite B/análise , Humanos , RNA Viral/análise
18.
J Med Microbiol ; 55(Pt 6): 715-20, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16687589

RESUMO

Infection with human papillomavirus (HPV) is the main cause of cervical cancer, the principal cancer in women in most developing countries. Molecular epidemiologic evidence clearly indicates that certain types of HPV are the principal cause of invasive cervical cancer and cervical intraepithelial neoplasia. Comprehensive, high-throughput typing assays for HPV, however, are not currently available. By combining L1 consensus PCR and multiplex hybridization using a Luminex xMAP system-based suspension array, the authors developed a rapid high-throughput assay, the HPV DNA suspension array (HPV-SA), capable of simultaneously typing 26 HPVs, including 18 high-risk HPV genotypes and eight low-risk HPV genotypes. The performance of the HPV-SA applied to 26 synthetic oligonucleotide targets was evaluated. The HPV-SA system perfectly discriminated 18 high-risk HPV targets from eight low-risk HPV targets. To assess the clinical applicability of the assay, the HPV-SA was performed with 133 MY09/MY11 primer set-mediated PCR (MY-PCR)-positive clinical specimens; of the 133 samples, 121 were positive by HPV-SA. Both single and multiple types were easily identified. The authors believe that improvement of the assay may be useful for epidemiological studies, cancer-screening programmes, the monitoring of therapeutic interventions, and the evaluation of the efficacy of HPV vaccine trials.


Assuntos
Técnicas de Tipagem Bacteriana/métodos , Colo do Útero/virologia , Papillomaviridae/classificação , Papillomaviridae/genética , Reação em Cadeia da Polimerase/métodos , Sequência de Bases , DNA Viral/genética , Feminino , Genótipo , Humanos , Técnicas In Vitro , Epidemiologia Molecular , Papillomaviridae/isolamento & purificação , Papillomaviridae/patogenicidade , Infecções por Papillomavirus/virologia
19.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 34(2): 98-103, 2005 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15812880

RESUMO

OBJECTIVE: To construct a cDNA library from human liver tissue of cirrhosis. METHODS: The total RNA from human liver tissue of cirrhosis was extracted using Trizol method, and the mRNA was purified using mRNA purification kit. SMART technique and CDSIII/3' primer were used for first-strand cDNA synthesis. Long distance PCR was then used to synthesize the double-strand cDNA that was then digested by proteinase K and Sfi I, and was fractionated by CHOMA SPIN-400 column. The cDNA fragments longer than 0.4 kb were collected and ligated to lambdaTripl Ex2 vector. Then lambda-phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries was strictly checked by conventional titer determination. Eleven plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. RESULTS: The titers of unamplifed and amplified libraries were 1.03 x 10(6) pfu/ml and 1.36 x 10(9) pfu/ml respectively. The percentages of recombinants from both libraries were 97.24 % in unamplified library and 99.02 % in amplified library. The lengths of the inserts were 1.02 kb in average (36.36 % 1 approximately equals 2 kb and 63.64 % 0.5 approximately equals 1.0 kb). CONCLUSION: A high quality cDNA library from human liver tissue of cirrhosis was constructed successfully, which can be used for screening and cloning new special genes associated with the occurrence of cirrhosis.


Assuntos
DNA Complementar/biossíntese , Biblioteca Gênica , Hepatite B Crônica/genética , Cirrose Hepática/genética , Cirrose Hepática/virologia , Hepatite B Crônica/complicações , Hepatite B Crônica/virologia , Humanos , Reação em Cadeia da Polimerase , Recombinação Genética , Transcrição Genética/genética
20.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 34(2): 104-9, 2005 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-15812881

RESUMO

OBJECTIVE: To inhibit HBV core antigen gene expression with plasmid-based RNAi. METHODS: The shRNA expression vector targeting HBV core antigen gene was designed and constructed. Human embryonic kidney cell line AD293 was co-transfected with HBcAg-EGFP fusion protein expression vector and shRNA expression vector transiently, and the cells without shRNA-transfection and with non-specific shRNA transfection were used as controls. Inhibitory effect of RNAi was detected by fluorescence-activated cell sorting (FACS) and real-time fluorescence quantificational RT-PCR. RESULTS: HBV core antigen gene expression in AD293 was inhibited by shRNA, with the maximal inhibition rate of 76 % measured by FACS and of 63.1 % by real-time PCR. CONCLUSION: Effective inhibition of HBV core antigen gene expression by plasmid-based RNAi provides an alternative for anti-HBV study in vitro, which has potential clinical application.


Assuntos
Regulação Viral da Expressão Gênica , Antígenos do Núcleo do Vírus da Hepatite B/biossíntese , Rim/virologia , Interferência de RNA/fisiologia , Linhagem Celular , Embrião de Mamíferos , Proteínas de Fluorescência Verde/biossíntese , Proteínas de Fluorescência Verde/genética , Antígenos do Núcleo do Vírus da Hepatite B/genética , Humanos , Rim/citologia , Plasmídeos , Reação em Cadeia da Polimerase , RNA Mensageiro/genética , Transfecção
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