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1.
Arthroscopy ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32057988

RESUMO

PURPOSE: To evaluate the feasibility of arthroscopic reduction percutaneous fixation (ARPF) in the treatment of isolated medial malleolar fracture and to compare the results with those of conventional open reduction internal fixation (ORIF). METHODS: This prospective study enrolled 77 patients with isolated medial malleolar fracture between November 2011 and February 2016. The patients were assigned to the ARPF (n = 34) and ORIF (n = 43) groups. The Olerud-Molander Ankle Score (OMAS), ankle range of motion (ROM), visual analog scale, and radiographic evaluation were determined at the scheduled follow-up. RESULTS: In the ARPF group, 11 of 34 (32.4%) patients had chondral lesions. Tears of the deltoid ligament and anterior inferior tibiofibular ligament were noted in 3 (8.8%) and 15 (44.1%) patients, respectively. The mean follow-up was 5 years. The mean OMAS was higher in the ARPF group than in the ORIF group. The differences were statistically significant at 6 months (80.2 ± 4.0 for ARPF vs. 77.2 ± 4.1 for ORIF, P = 0.005) and 1 year (92.9 ± 4.9 vs. 88.1 ± 4.6, P < 0.001), but not at the latest follow-up (P = 0.081). Ankle ROM was markedly improved in the ARPF group unlike in the ORIF group at 6 months (dorsiflexion: P = 0.025; plantar flexion: P < 0.001) and 1 year (dorsiflexion and plantar flexion: P < 0.001). The improvement remained present at the latest follow-up in plantar flexion (P = 0.001) but not in dorsiflexion (P = 0.354). CONCLUSIONS: Arthroscopy-assisted reduction is a feasible alternative modality with superior short-term outcomes for treating isolated medial malleolar fracture, but its superiority may be attenuated in the intermediate term.

2.
Pathol Oncol Res ; 2020 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-31989402

RESUMO

Glioblastoma multiforme (GBM) is the most malignant form of the brain tumors. EGFR variant III (EGFRvIII) is expressed in about 30% of GBM specimens, but not expressed in normal brain tissues. Therefore, EGFRvIII protein offers an ideal CAR-T therapeutic target for EGFRvIII-positive GBM patients. PD-L1 is expressed in a variety of cancer cells, including GBM. Tumor-associated PD-L1 can bind to PD-1 on T cells and promote apoptosis of T cells, thus suppressing the anti-cancer immune response. In our current studies, PD-1WT EGFRvIII-CAR-T cells and PD-1KD EGFRvIII-CAR-T cells were generated. Cytokine production and lytic activity of these two CAR-T cells against to PD-L1WT EGFRvIII+ U373 cells or PD-L1KO EGFRvIII+ U373 cells were evaluated. The results showed that PD-1KD EGFRvIII-CAR-T cells and PD-1WT EGFRvIII-CAR-T cells showed same levels of interferon-γ (IFN-γ) and interleukin-2 (IL-2) production as well as cytolytic activity against PD-L1KO EGFRvIII+ U373 cells; however, PD-1KD EGFRvIII-CAR-T cells exhibited higher levels of IFN-γ and IL-2 production as well as cytolytic activity against PD-L1+ EGFRvIII+ U373 cells than that of PD-1WT EGFRvIII-CAR-T cells. PD-1KD EGFRvIII-CAR-T cells also exhibited higher anti-glioma activity and longer survival in mice in vivo than that of PD-1WT EGFRvIII-CAR-T cells. Taken together, our findings indicate that PD-1 knockout enhances lytic activity of EGFRvIII-CAR-T cells against PD-L1+ EGFRvIII+ GBM cells. These might provide a new insight into strategy of GBM CAR-T cell therapy.

3.
Artigo em Inglês | MEDLINE | ID: mdl-31925688

RESUMO

This study examined the influence of biochar addition on fungal community during composting of cow manure using high-throughput sequencing. Two treatments were set up, including compost of cow manure plus 10% biochar (BC) and cow manure compost without biochar (CK). Fungal community composition varied obviously during composting in both treatments, and main fungi included Aspergillus, Myriococcum, Thermomyces, Mycothermus, Scedosporium, Cladosporium, and unclassified Microascaceae. Fungal community composition was altered by biochar during composting, especially during the thermophilic and the cooling phase, promoting Aspergillus and Myriococcum while inhibiting unclassified Microascaceae and Thermomyces. Based on linear discriminant analysis effect size analysis, common indicator groups were detected in both composts; however, specific indicator groups were also found in BC treatment, including Clavicipitaceae, Tremellales, Gibberella, and Coprinopsis. Canonical correspondence analysis (CCA) indicated that moisture content, organic matter, C/N, and pH had significant correlation (p < 0.05) with fungal composition in both treatments. However, in compost added with biochar, temperature was not an important factor affecting fungal community (p > 0.05).

4.
Ecotoxicol Environ Saf ; 191: 110161, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31954219

RESUMO

Denitrification and nitrification processes are the two prominent pathways of nitrogen (N) transformation in composting matrix. This study explored the dynamics of denitrifying and nitrifying bacteria at different composting stages of cow manure and corn straw using functional gene sequencing at DNA and cDNA levels. Corresponding agreement among OTUs, NMDS, mental test and network analyses revealed that functional bacteria community compositions and responses to physicochemical factors were different at DNA and cDNA levels. Specifically, some OTUs were detected at the DNA level but were not observed at cDNA level, differences were also found in the distribution patterns of nitrifying and denitrifying bacteria communities at both levels. Furthermore, co-occurrence network analysis indicated that Pseudomonas, Paracoccus and Nitrosomonas were identified as the keystone OTUs at the DNA level, while Paracoccus, Agrobacterium and Nitrosospira were keystone OTUs at the cDNA level. Mantel test revealed that TN, C/N and moisture content significantly influenced both the denitrifying bacteria and ammonia-oxidizing bacteria (AOB) communities at the DNA level. NO3--N, NH4+-N, TN, C/N, and moisture content only registered significant correlation with the nosZ-type denitrifiers and ammonia-oxidizing bacteria (AOB) communities at the cDNA level. Structural equation model (SEM) showed that TN, NH4+-N, and pH were direct and significantly influenced the gene abundance of denitrifying bacteria. Howbeit, TN, NH4+-N, and NO3--N had significant direct effects on amoA gene abundance.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31746033

RESUMO

In this study, nutrient loss, the direct and indirect relationship between period, compost types, temperature, total nitrogen (TN), nitrate nitrogen (NO3 - -N), ammonia nitrogen (NH4 + -N), microbial biomass carbon (MBC) and nitrogen (MBN) were investigated during composting of cattle manure-maize straw mixture. This study findings revealed that biochar addition lowered NH4 + -N but did not increase NO3 - -N concentrations unlike no biochar piles during composting. The first-order kinetic models showed that biochar accelerated organic matter (OM) degradation, improved N mineralization, consequently reducing TN losses by 13.6% and OM losses by 12.66%. Transformation ratio of MBC/MBN, coupled with other chemical components of the entire microbial community suggested a shift in the microbial succession and diversity during composting from the dominant bacteria and actinomycetes to fungi. The Structural equation model and path coefficient revealed temperature to be the main factor mediating the evolution of MBC and MBN in composting. The physicochemical variables, phytotoxicity and final product quality revealed that biochar incorporation to the composting feedstock is an ideal material for mitigating problems of TN and OM losses in composting and ultimately enhancing the fertility potential of the final compost product. This article is protected by copyright. All rights reserved.

6.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 36(11): 1133-1135, 2019 Nov 10.
Artigo em Chinês | MEDLINE | ID: mdl-31703144

RESUMO

OBJECTIVE: To identify a novel human leukocyte antigen (HLA) B allele in a Chinese Han individual and construct its three-dimensional structure. METHODS: The initial HLA genotyping was performed by PCR-sequence-based typing (PCR-SBT). The ambiguous allele was confirmed with single-strand DNA sequencing. The DNA sequence was analyzed to identify the difference between the novel allele and its closest matching allele. Finally, the three-dimensional molecular structure of the novel allele was constructed using a Swiss-Model. RESULTS: One allele of the subject at the HLA-B locus was B*44:03:01, whilst the other was a novel allele which differed from the closest matching allele B*51:01:01:01 by nucleotide (nt) 329 A to C in exon 2, resulting in an amino acid change at codon 86 (p.Asn86Thr). CONCLUSION: A novel HLA-B allele has been identified and officially named as HLA-B*51:159 by the WHO Nomenclature Committee for Factors of the HLA System. The three-dimensional structure of B*51:159 was simulated.


Assuntos
Grupo com Ancestrais do Continente Asiático , Antígenos HLA-B/química , Antígenos HLA-B/genética , Alelos , Sequência de Bases , Humanos , Conformação Molecular , Análise de Sequência de DNA
7.
Med Sci Monit ; 25: 4811-4818, 2019 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-31253757

RESUMO

BACKGROUND We aimed to investigate the role of PDCD4-mediated Akt signaling pathway in vascular endothelial cell injury caused by ischemia-reperfusion in the lower extremities. MATERIAL AND METHODS Ten rats were used as control, while 50 rats were used for creating disease models and were assigned to 5 groups: model group (no injection), NC group (injected with vectors containing PDCD negative control sequence), sh-PDCD4 group (injected with vectors containing sh-PDCD4 sequence), IGF-1 group (injected with IGF-1), and sh-PDCD4+IGF-1 group (injected with IGF-1 and vectors containing sh-PDCD4 sequence). RESULTS Compared with the control group, the expression levels of PDCD4 mRNA and protein, as well as levels of circulating endothelial cells, von Willebrand factor, thrombomodulin, and malondialdehyde, increased in the other 5 groups, while the mRNA and protein expression levels of Akt and eNOS, the protein expression levels of p-Akt and p-eNOS, and superoxide dismutase content decreased in these groups (all P<0.05). Compared with the model group, the sh-PDCD4 and sh-PDCD4+1GF-1 groups had lower mRNA and protein expressions of PDCD4 (all P<0.05), whereas the IGF-1 group had similar levels (all P>0.05). These 3 groups had lower levels of circulating endothelial cells, von Willebrand factor, thrombomodulin, and malondialdehyde, and higher mRNA and protein expressions of Akt and eNOS, protein expressions of p-Akt and p-eNOS, and superoxide dismutase content (all P<0.05). The NC group did not differ from the model group (all P>0.05). CONCLUSIONS PDCD4 gene silencing can activate the Akt signaling pathway and attenuate vascular endothelial cell injury caused by ischemia-reperfusion in the lower extremities in rats.


Assuntos
Proteínas Reguladoras de Apoptose/metabolismo , Células Endoteliais/metabolismo , Extremidade Inferior/irrigação sanguínea , Proteínas Proto-Oncogênicas c-akt/metabolismo , Traumatismo por Reperfusão/metabolismo , Traumatismo por Reperfusão/patologia , Animais , Apoptose/genética , Proteínas Reguladoras de Apoptose/genética , Células Endoteliais/patologia , Inativação Gênica , Fator de Crescimento Insulin-Like I/metabolismo , Extremidade Inferior/patologia , Masculino , MicroRNAs/genética , Fosfatidilinositol 3-Quinases/metabolismo , RNA Mensageiro/metabolismo , Ratos , Ratos Sprague-Dawley , Traumatismo por Reperfusão/genética , Transdução de Sinais
8.
Front Microbiol ; 10: 529, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30936861

RESUMO

In composting system, the composition of microbial communities is determined by the constant change in the physicochemical parameters. This study explored the dynamics of bacterial and fungal communities during cow manure and corn straw composting using high throughput sequencing technology. The relationships between physicochemical parameters and microbial community composition and abundance were also evaluated. The sequencing results revealed the major phyla included Proteobacteria, Bacteroidetes, Firmicutes, Chloroflexi and Actinobacteria, Ascomycota, and Basidiomycota. Linear discriminant analysis effect size (LEfSe) illustrated that Actinomycetales and Sordariomycetes were the indicators of bacteria and fungi in the maturation phase, respectively. Mantel test showed that NO3 --N, NH4 +-N, TN, C/N, temperature and moisture content significantly influenced bacterial community composition while only TN and moisture content had a significant effect on fungal community structure. Structural equation model (SEM) indicated that TN, NH4 +-N, NO3 --N and pH had a significant effect on fungal abundance while TN and temperature significantly affected bacterial abundance. Our finding increases the understanding of microbial community succession in cow manure and corn straw composting under natural conditions.

9.
J Craniofac Surg ; 30(2): e131-e135, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30550449

RESUMO

OBJECTIVE: At present, large cranial function area arteriovenous malformation (fAVM) is mainly treated by craniotomy with a high risk of operation and high disability. In recent years, with the continuous improvement of the neural intervention technology, fractionated transcatheter arterial embolization (fTAE) may be used to treat the fAVM instead of surgical treatment. However, its effectiveness for treating fAVM has never been explored. The authors hypothesized that fTAE can be effective in the treatment of fTAE. METHODS: A retrospective study was conducted in 229 cases of large fAVM in multicenter hospitals. Among them, 103 cases were performed fTAE and the other 126 cases were carried on minimally invasive craniotomy (MIC). Clinically relevant symptomatic improvement and complications were compared between 2 groups. RESULTS: Complete resection rate of arteriovenous malformation in MIC group (100%) was significantly higher than that of complete embolization rate in fTAE group (77.7%) (P = 0.000). However, the incidence of postoperative complications includes decreased limb muscle strength (P = 0.001), sensory loss of extremities (P = 0.003), visual field defect (P = 0.025) that were lower in fTAE group and remission rate of headache (P = 0.012) but not epilepsy (P = 0.952) was higher in fTAE group compared with that in MIC group. After 1 year follow-up, the rebleeding rate in the fTAE treatment group was 4.85%, higher than that in MIC group (0%) (P = 0.000). CONCLUSIONS: Fractionated transcatheter arterial embolization therapy is beneficial for reducing the postoperative complications and preoperative symptoms of fAVM, but not for recurrence rate.


Assuntos
Malformações Arteriovenosas/complicações , Malformações Arteriovenosas/terapia , Hemorragia Cerebral/etiologia , Embolização Terapêutica/métodos , Procedimentos Cirúrgicos Vasculares/métodos , Adulto , Malformações Arteriovenosas/cirurgia , Encéfalo/irrigação sanguínea , Craniotomia/efeitos adversos , Embolização Terapêutica/efeitos adversos , Epilepsia/etiologia , Extremidades , Feminino , Cefaleia/etiologia , Humanos , Masculino , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos/efeitos adversos , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Debilidade Muscular/etiologia , Complicações Pós-Operatórias/etiologia , Recidiva , Estudos Retrospectivos , Distúrbios Somatossensoriais/etiologia , Resultado do Tratamento , Procedimentos Cirúrgicos Vasculares/efeitos adversos , Transtornos da Visão/etiologia
10.
Int J Biometeorol ; 63(5): 679-686, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-28493142

RESUMO

The tree-ring cellulose oxygen isotopes (δ18O) for four trees of Hippophae tibetana and four trees of Abies georgei growing in different locations around the terminal moraine in Xincuo from 1951 to 2010 were measured to explore its potential for reconstructing climatic variations in the southeastern Tibetan Plateau. The mean and standard deviation of tree-ring δ18O at different heights do not have significant differences, and there are no significant differences in the mean and standard deviation of tree-ring δ18O between trees near the brook and trees at the top of moraine, indicating that we can collect samples for tree-ring δ18O analysis regardless of sampling heights and that the micro-environment does not affect tree-ring δ18O significantly. The mean inter-series correlations of cellulose δ18O for A. georgei/H. tibetana are 0.84/0.93, and the correlation between δ18O for A. georgei and H. tibetana is 0.92. The good coherence between inter-tree and inter-species cellulose δ18O demonstrates the possibility of using different species to develop a long chronology. Correlation analysis between tree-ring δ18O and climate parameters revealed that δ18O for A. georgei/H. tibetana had negative correlations (r = -0.62/r = -0.69) with relative humidity in July-August, and spatial correlation revealed that δ18O for A. georgei/H. tibetana reflected the regional Standardized Precipitation Evapotranspiration Index (29°-32° N, 88°-98° E). In addition, tree-ring δ18O in Xincuo has a significant correlation with tree-ring δ18O in Bhutan. The results indicate that cellulose δ18O for A. georgei and H. tibetana in Xincuo is a good proxy for the regional hydroclimate.


Assuntos
Abies , Celulose/análise , Hippophae , Isótopos de Oxigênio/análise , Clima , Umidade , Projetos de Pesquisa , Tibet , Madeira/análise
11.
J Biotechnol ; 289: 26-30, 2019 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-30428383

RESUMO

Pichia pastoris is subjected to strong oxidative stress in the methanol induction phase. The oxidative stress inflicts severe injury to yeast cells, which causes cell death and reduces protein expression ability. N-acetyltransferase in Saccharomyces cerevisiae can protect yeast cells from damage caused by decreasing reactive oxygen species (ROS) in oxidative pressure environments such as ethanol treatment, freeze-thawing, or heat shock. In this study, N-acetyltransferase from P. pastoris (PpMpr1) was overexpressed for the first time to improve the anti-oxidative stress ability to protect cells from strong ROS damage during the methanol induction phase. Cell viability of the PpMpr1 overexpression strain increased significantly, while biomass was increased by 22.7% at high dissolved oxygen (DO). At the same time, the heterologous α-glucosidase (AGL) expression level at 25% DO was increased by 21.5%. The AGL degradation was greatly relieved in the fermentation supernatant of the PpMpr1 overexpression strain. This study shows that PpMpr1 has a great potential for improvement of anti-oxidative stress ability in P. pastoris and provides a promising recombinant microorganism for industrial production of proteins.


Assuntos
Acetiltransferases/metabolismo , Proteínas Fúngicas/metabolismo , Pichia/enzimologia , alfa-Glucosidases/metabolismo , Acetiltransferases/genética , Proteínas Fúngicas/genética , Pichia/genética , Espécies Reativas de Oxigênio/metabolismo , Proteínas Recombinantes/metabolismo
12.
Orthop Surg ; 10(4): 296-305, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30402963

RESUMO

OBJECTIVE: To compare postoperative imaging results, clinical outcomes and complications between the multifidus muscle bundle (MMB) approach and the conventional open (CO) approach in one-level posterior lumbar interbody fusion (PLIF). METHODS: Based on the inclusion and exclusion criteria, 201 of 351 patients in our hospital were enrolled in this prospective study and underwent MMB-PLIF or CO-PLIF randomly: 111 patients in the MMB-PLIF group and 90 patients in the CO-PLIF group. A total of 100 patients failed to be followed up in the following 7-9 years. Therefore, in this study, 52 patients of the MMB group and 49 patients of the CO group were included. We evaluated the differences in terms of multifidus atrophy rate, intervertebral disc height and segmental lordosis restoration of the operation segment, lumbar lordosis restoration, fusion rate, visual analogue scale (VAS) for back and leg pain, Oswestry disability index (ODI), complication rates, and patient satisfaction rates between the two groups. Correlation between multifidus muscle degeneration and the incidence of complications was investigated, and we compared the multifidus muscle degeneration rate between patients with or without intractable back pain or adjacent segment degeneration. RESULTS: There were no significant differences in age, sex, body mass index (BMI), diagnosis, segments distribution, and mean follow-up time between the MMB-PLIF group and the CO-PLIF group. In addition, no differences regarding sex, age, or BMI were found between the lost follow-up group and the successful follow-up group. In regard to imaging and clinical evaluation, at the final follow-up, there were significant differences in multifidus atrophy rates (27.0% ± 6.8% vs 38.7% ± 10.9%), lumbar lordosis restoration (4.6° ± 2.5° vs 3.0° ± 1.9°), postoperative VAS for back pain (1.1 ± 0.9 vs 1.8 ± 1.2), ODI (7.7 ± 5.0 vs 12.4 ± 6.7), and patient satisfaction rates (86.5% vs 61.2%) between MMB-PLIF and CO-PLIF groups. However, there were no significant differences in segmental lordosis, intervertebral height restoration, postoperative VAS for leg pain or fusion rate between the two groups. In regards to complications, there were significant differences in the incidence of adjacent segment degeneration (3.8% vs 14.3%), intractable back pain (3.8% vs 22.4%), and residual neurological symptoms (5.8% vs 20.4%) between the two groups (P < 0.05) at the final follow-up. In addition, patients with adjacent segment degeneration and intractable back pain were observed with more significant multifidus muscle atrophy than those without these two complications (31.9% ± 1.1% vs 39.6% ± 2.1% and 30.9% ± 1.1% vs 42.8% ± 2.1%). CONCLUSION: Compared with CO-PLIF, MMB-PLIF had advantages in relation to protection of the multifidus muscle, better maintenance of lumbar lordosis, reduced lower back pain and ODI score, fewer complications, and a higher patient satisfaction rate. Protection of the multifidus muscle in lumbar surgery is an important aspect of minimally invasive surgery.


Assuntos
Vértebras Lombares/cirurgia , Músculos Paraespinais/cirurgia , Fusão Vertebral/métodos , Idoso , Dor nas Costas/etiologia , Feminino , Humanos , Vértebras Lombares/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Atrofia Muscular/etiologia , Medição da Dor/métodos , Satisfação do Paciente , Parafusos Pediculares , Complicações Pós-Operatórias , Estudos Prospectivos , Radiografia , Fusão Vertebral/efeitos adversos
13.
J Cell Biochem ; 2018 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-30485503

RESUMO

miR-145 has been found to be significantly downregulated in gliomas, and overexpression of miR-145 increases glioma cell apoptosis and enhances chemosensitivity or herpes simplex virus thymidine kinase gene therapy. However, the correlation between miR-145 and the clinical prognosis of glioblastomas has never been explored. In this study, a retrospective study was conducted in 86 cases of patients with glioblastoma after neurosurgery combined with chemoradiotherapy, and 36 cases with traumatic brain injury. Our results showed that miR-145 was significantly lower in glioblastoma tissues than that in normal brain tissue (P < 0.05). Furthermore, miR-145 was lower in patients with lower Karnofsky Performance Scale (KPS) scores than in patients with higher KPS scores ( P < 0.05). Cox Regression analysis showed that low miR-145 expression was associated with poor patient survival ( P < 0.05). These data suggested that patients with glioblastoma with lower miR-145 expression are prone to shorter overall survival.

14.
Proc Natl Acad Sci U S A ; 115(42): E9962-E9970, 2018 10 16.
Artigo em Inglês | MEDLINE | ID: mdl-30266793

RESUMO

Genomic imprinting is a form of epigenetic regulation resulting in differential gene expression that reflects the parent of origin. In plants, imprinted gene expression predominantly occurs in the seed endosperm. Maternal-specific DNA demethylation by the DNA demethylase DME frequently underlies genomic imprinting in endosperm. Whether other more ubiquitously expressed DNA demethylases regulate imprinting is unknown. Here, we found that the DNA demethylase ROS1 regulates the imprinting of DOGL4 DOGL4 is expressed from the maternal allele in endosperm and displays preferential methylation and suppression of the paternal allele. We found that ROS1 negatively regulates imprinting by demethylating the paternal allele, preventing its hypermethylation and complete silencing. Furthermore, we found that DOGL4 negatively affects seed dormancy and response to the phytohormone abscisic acid and that ROS1 controls these processes by regulating DOGL4 Our results reveal roles for ROS1 in mitigating imprinted gene expression and regulating seed dormancy.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/fisiologia , Metilação de DNA , Proteínas de Ligação a DNA/metabolismo , Impressão Genômica , Proteínas Nucleares/metabolismo , Dormência de Plantas , Sementes/fisiologia , Fatores de Transcrição/metabolismo , Proteínas de Arabidopsis/genética , DNA de Plantas/genética , Proteínas de Ligação a DNA/genética , Epigênese Genética , Regulação da Expressão Gênica de Plantas , Proteínas Nucleares/genética , Fatores de Transcrição/genética
15.
Cell Tissue Bank ; 19(4): 667-679, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30069708

RESUMO

To observe the effect of DMEM/F12 pegylated with methoxy polyethylene glycol succinimidyl propionate (mPEG-SPA) on the biophysical and immune characteristics of the rat cornea preserved in it. Corneal grafts were harvested from Wistar rat and preserved in the DMEM/F12 plus mPEG-SPA, DMEM/F12 without mPEG-SPA, and standard Optisol-GS solution at 4 °C for 14 days, referred as plus-PEG, minus-PEG, and Optisol grafts, respectively. The biophysical properties of those grafts, including transmittance, thickness, water content, and biomechanics were investigated. The survival of those grafts was observed in the high-risk corneal transplantation model. Transmittance and biomechanics did not show any differences among those grafts. Thickness and water content of plus-PEG grafts were slightly improved. Proliferation and activation of lymphocytes were lower while they were incubated with plus-PEG grafts, compared with minus-PEG grafts and Optisol grafts. The mean survival time was significantly prolonged in plus-PEG grafts. DMEM/F12 solution plus mPEG-SPA improved the survival of corneal grafts and maintained the comparative biophysical characteristics of them, compared with the standard preservation solution.


Assuntos
Córnea/efeitos dos fármacos , Soluções para Preservação de Órgãos/farmacologia , Polietilenoglicóis/farmacologia , Animais , Fenômenos Biomecânicos , Fenômenos Biofísicos , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Transplante de Córnea , Citocinas/metabolismo , Endotélio/transplante , Feminino , Sobrevivência de Enxerto/efeitos dos fármacos , Linfócitos/efeitos dos fármacos , Linfócitos/metabolismo , Ratos Sprague-Dawley , Ratos Wistar
16.
Glob Chang Biol ; 24(11): 5549-5559, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30153361

RESUMO

Among forest ecosystems, the alpine treeline ecotone can be considered to be a simplified model to study global ecology and climate change. Alpine treelines are expected to shift upwards in response to global warming given that tree recruitment and growth are assumed to be mainly limited by low temperatures. However, little is known whether precipitation and temperature interact to drive long-term Himalayan treeline dynamics. Tree growth is affected by spring rainfall in the central Himalayan treelines, being good locations for testing if, in addition to temperature, precipitation mediates treeline dynamics. To test this hypothesis, we reconstructed spatiotemporal variations in treeline dynamics in 20 plots located at six alpine treeline sites, dominated by two tree species (birch, fir), and situated along an east-west precipitation gradient in the central Himalayas. Our reconstructions evidenced that treelines shifted upward in response to recent climate warming, but their shift rates were primarily mediated by spring precipitation. The rate of upward shift was higher in the wettest eastern Himalayas, suggesting that its ascent rate was facilitated by spring precipitation. The drying tendency in association with the recent warming trends observed in the central Himalayas, however, will likely hinder an upslope advancement of alpine treelines and promote downward treeline shifts if moisture availability crosses a critical minimum threshold. Our study highlights the complexity of plant responses to climate and the need to consider multiple climate factors when analyzing treeline dynamics.


Assuntos
Aquecimento Global , Dispersão Vegetal , Chuva , Árvores/fisiologia , Altitude , Nepal , Fatores de Tempo
17.
CMAJ Open ; 6(3): E254-E260, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30012644

RESUMO

BACKGROUND: Alberta is considering capping daily fee-for-service physician billings, but little is known about high-volume practice in the province and its impact on patient health outcomes. In this initial study, we conducted a descriptive analysis of general practitioners' patient volumes and billing practices in relation to associated practitioner demographic characteristics. METHODS: We conducted a retrospective descriptive analysis of the associations of practitioner characteristics, including full-time versus non-full-time practice, provider sex, years in practice, geographic location and international medical graduate status, with high-volume (> 50 visits/d) practice using general practice billing data from 2011 to 2016. Use of general practitioner service codes was described and compared by general practitioner volume status, with adjustment for physician demographic characteristics and geographic parameters. RESULTS: We included 3465 general practitioners practising fee-for-service in Alberta between 2011 and 2016, of whom 233 (6.7%) were identified as high-volume providers. Physicians who had been in practice longer (odds ratio [OR] 1.04 per year, 95% confidence interval [CI] 1.02-1.05) and international medical graduates (OR 1.89, 95% CI 1.40-2.54) were more likely to exceed 50 patient visits/day. Female physicians were less likely to exceed 50 patient visits/day (OR 0.14, 95% CI 0.07-0.28). Rural practice location was negatively associated with high-volume practice (OR 0.87, 95% CI 0.79-0.95) when we controlled for zone within the province. Zone 5 (North) was associated with high-volume practice (OR 1.95, 95% CI 1.06-3.58). Less than full-time practice was prevalent (1836 providers [53.0%]). High-volume general practitioners billed fewer service codes requiring longer visits, except for the most highly remunerated code (patients with complex health issues). INTERPRETATION: These results can inform policy-makers when considering payment system changes. Our next step is to examine the association of high-volume practice with outcomes important to patients, such as evidence of treatment failure (emergency department visits and hospital admissions) for conditions sensitive to primary care management.

19.
Eur Spine J ; 27(8): 1757-1766, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29909551

RESUMO

PURPOSE: To evaluate the effects of leptin/leptin receptor (LepR) combined with mechanical stress on the development of ossification of the posterior longitudinal ligament (OPLL), which is a disease characterized by ectopic bone formation of the posterior longitudinal ligament (PLL) and can lead to radiculopathy and myelopathy. METHODS: Six human samples of the PLL were analyzed for the expression of leptin and LepR by RT-PCR and western blotting. PLL cells were stimulated with leptin and mechanical stress delivered via a Flexcell tension system, and osteogenic differentiation was evaluated by RT-PCR and western blotting analysis of osteogenic marker expression as well as by alkaline phosphatase (ALP) staining and alizarin red S staining. Activation of mitogen-activated protein kinase (MAPK), Janus kinase (JAK) 2-signal transducer, activator of transcription (STAT) 3 and phosphatidylinositol 3-kinase (PI3K)-Akt was evaluated by western blotting. RESULTS: Samples from the OPLL group had higher LepR mRNA and protein levels and lower leptin levels than those from healthy controls. Exposure to leptin and Flexcell increased the number of ALP-positive cells and calcium nodules in a dose-dependent manner; this effect was accompanied by upregulation of the osteogenic markers osteocalcin, runt-related transcription factor 2 (RUNX2) and osteopontin. Extracellular signal-regulated kinase, P38 MAPK, JAK2, STAT3, PI3K and Akt signaling, was also activated by the combined effects of leptin and mechanical stress. CONCLUSIONS: Leptin and LepR are differentially expressed in OPLL tissues, and the combined use of leptin/LepR and mechanical stress promotes osteogenic differentiation of PLL cells via MAPK, JAK2-STAT3 and PI3K/Akt signaling. These slides can be retrieved under Electronic Supplementary Material.


Assuntos
Leptina/metabolismo , Ossificação do Ligamento Longitudinal Posterior/metabolismo , Ossificação Heterotópica/metabolismo , Receptores para Leptina/metabolismo , Estresse Mecânico , Fosfatase Alcalina/metabolismo , Western Blotting , Técnicas de Cultura de Células , Diferenciação Celular , Humanos , Ligamentos Longitudinais/citologia , Ligamentos Longitudinais/metabolismo , Ligamentos Longitudinais/patologia , Ossificação do Ligamento Longitudinal Posterior/etiologia , Ossificação Heterotópica/etiologia , Reação em Cadeia da Polimerase em Tempo Real , Transdução de Sinais
20.
World Neurosurg ; 113: 198-203, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29482006

RESUMO

OBJECTIVE: Obvious skin flap collapse is often accompanied by reduced neurologic recovery after decompressive craniectomy. This study explored the feasibility of early cranioplasty (EC) in patients with obvious bilateral frontotemporal bone window (BFBW) collapse after decompressive craniectomy. METHODS: Patients with obvious BFBW collapse who underwent EC or traditional cranioplasty (TC) were divided into 3 groups according to their preoperative Glasgow Coma Scale (GCS) scores. The indexes, including postoperative incision healing, salivation symptoms, postoperative infection, and 6-month postoperative follow-up after EC or TC, were compared in each group. RESULTS: Two of 32 patients with GCS scores of 3 to 8 points experienced poor healing of the scalp incision after EC, whereas no TC patients had poor healing. Incision healing significantly differed between these 2 groups (P > 0.05), and long-term prognoses based on GOS scores were the same after a 6-month postoperative follow-up (P > 0.05). In patients with GCS scores of 9 to 12 points, salivation improved by 84.2% and 17.6% in the EC and TC groups, respectively (P < 0.05) after a mean follow-up time of 6 months. Similarly, positive neurologic recovery rates (GOS score 4-5 points) were higher in the EC group (88.9%) than in the TC group (60.0%) (P < 0.05) and did not differ between the EC (79.3%) and TC (80.6%) groups in patients with GCS scores of 13 to 15 points (P > 0.05). However, salivation improved by 86.7% in the EC group but by only 12.5% in the TC group (P < 0.05). CONCLUSION: We therefore recommend EC for patients with obvious BFBW collapse and GCS scores ≥9.


Assuntos
Craniectomia Descompressiva/tendências , Osso Frontal/diagnóstico por imagem , Complicações Pós-Operatórias/diagnóstico por imagem , Complicações Pós-Operatórias/cirurgia , Retalhos Cirúrgicos/tendências , Osso Temporal/diagnóstico por imagem , Adulto , Craniectomia Descompressiva/métodos , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Retalhos Cirúrgicos/efeitos adversos , Telas Cirúrgicas/estatística & dados numéricos
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