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1.
Mikrochim Acta ; 189(5): 212, 2022 05 04.
Artigo em Inglês | MEDLINE | ID: mdl-35507110

RESUMO

Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, which is known for its rapid process, simple operation, high amplification efficiency, and excellent sensitivity. However, most of the existing heating methods are external for completion of molecular amplification with possibility of contamination of specimens. The present research provided an internal heating method for LAMP using magnetic nanoparticles (MNPs), which is called nano-LAMP. Near-infrared light with an excitation wavelength of 808 nm was employed as the heating source; hydroxy naphthol blue (HNB) was used as an indicator to conduct methodological research. We demonstrate that the best temperature was controlled at a working power of 2 W and 4.8 µg/µL concentration of nanoparticles. The lowest limit for the detection of HPV by the nano-LAMP method is 102 copies/mL, which was confirmed by a gel electrophoresis assay. In the feasibility investigation of validated clinical samples, all 10 positive HPV-6 specimens amplified by nano-LAMP were consistent with conventional LAMP methods. Therefore, the nano-LAMP detection method using internal heating of MNPs may bring a new vision to the exploration of thermostatic detection in the future.


Assuntos
Calefação , Técnicas de Amplificação de Ácido Nucleico , DNA , Papillomavirus Humano 6 , Técnicas de Diagnóstico Molecular , Técnicas de Amplificação de Ácido Nucleico/métodos , Sensibilidade e Especificidade
2.
Artigo em Inglês | MEDLINE | ID: mdl-35564894

RESUMO

The purpose of this study was to analyze the health status and unmet healthcare needs, and the impact of related factors, of unwell migrants in Shanghai. A total of 10,938 respondents, including 934 migrants and 10,004 non-migrants, were interviewed in Shanghai's Sixth Health Service Survey. Descriptive statistics were utilized to present the prevalence of health status and unmet healthcare needs. Binary logistic regression analysis was performed to explore the relationships between predisposing factors, enabling factors, need factors, and health-related behavior and unmet healthcare needs in the Anderson health service utilization model. This study indicated the percentages of migrants having a fair or poor self-evaluated health status (21.09%) and suffering from chronic diseases (72.91%) were lower than those of non-migrants (28.34% and 88.64%, respectively). Migrants had higher percentages of unmet hospitalization needs (88.87%), unmet outpatient care needs (44.43%), and self-medication (23.98%) than those of non-migrants (86.24%, 37.95%, 17.97%, respectively). Migrants enrolled in Urban Employee Basic Medical Insurance were more likely to utilize hospitalization services (OR = 1.457) than those enrolled in other health insurances or uninsured. Need factors had impacts on unwell migrants' unmet healthcare needs. Other factors, including age and health behavior, were also found to significantly affect unwell migrants' unmet health service needs. Specific gaps continue to exist between unwell migrants and non-migrants regarding the accessibility of local health services. Flexible policies, such as enhancing the health awareness of migrants and eliminating obstacles for migrants to access medical services, should be implemented to provide convenient and affordable healthcare services to unwell migrants.

3.
Infect Drug Resist ; 15: 1889-1902, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35465249

RESUMO

Objective: This study aimed to investigate the characteristics of the non-human immunodeficiency virus (HIV) pneumocystis pneumonia (PCP) via the microbial composition of Pneumocystis jirovecii pneumonia in the lower respiratory tract in infants with severe pneumonia who were hospitalized in the study's pediatric intensive care unit (PICU). Methods: The clinical characteristics of 16 infants with non-HIV PCP (the PCP group) and 33 infants with severe pneumonia (the control group) who were hospitalized at the same time in the PICU were analyzed retrospectively. Using metagenomic next-generation sequencing (mNGS), the bronchoalveolar lavage fluid (BALF) of the two groups was analyzed, and the microbial results and clinical data were compared. Results: Compared with the control group, the infants in the PCP group had a lower incidence of cough (25% vs 78.8%; P < 0.05), a greater history of surgery (50.0% vs 39.1%; P < 0.05), and a more significant decrease in C3, C4, and CD4/CD8 ratios (all P < 0.05). The pathogenic bacteria in the BALF included P. jirovecii, respiratory syncytial virus, cytomegalovirus (CMV), and Staphylococcus aureus. The predominance of viral infection in the PCP group was significantly higher than in the control group (P < 0.05), especially CMV (43.5% vs 15.2%; P < 0.05). The top five symbiotic microorganisms detected in the BALF of the 49 infants were Streptococcus, Propionibacterium, Rothia, Staphylococcus, and Moraxella. There was no significant difference in the relative abundance of common symbiotic microorganisms between the two groups (all P > 0.05). Conclusion: Non-HIV PCP has a higher incidence in PICU infants with severe pneumonia, especially those with underlying diseases or who are immunocompromised, which are clinically difficult to treat. A BALF analysis using mNGS is helpful for early and clear diagnoses. It also helps to clarify the distribution of pathogenic and lower respiratory tract colonizing bacteria in infants with severe pneumonia.

4.
J Colloid Interface Sci ; 617: 585-593, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35303642

RESUMO

As an efficient non-precious metal catalyst for the oxygen evolution reaction (OER), phosphides suffer from poor electrical conductivity, so it is still a challenge to reasonably design their structures to further improve their conductivity and OER performances. Here, we present a novel Ni5P4/N-doped carbon@CoFeP/N-doped carbon composite (Ni5P4/NC@CoFeP/NC) as electrocatalysts for OER. This elaborate structure consists of Ni5P4/NC derived from Ni-MOF and CoFeP/NC derived from CoFe-Prussian blue analog MOF (Co-Fe PBA). The cube-like CoFeP/NC are scattered and uniformly coated on the sheet of Ni5P4/NC flowers. Among them, NC can enhance the conductivity of phosphides, while CoFeP/NC can increase the electrochemical active area, which benefit the properties of Ni5P4/NC@CoFeP/NC. Notably, the Ni5P4/NC@CoFeP/NC catalyst possesses outstanding OER performances with a low overpotential of 260 and 303 mV at a current density of 10 and 100 mA·cm-2, an ultra-low Tafel slope of 31.1 mV·dec-1 and excellent stability in 1 M KOH. XPS analysis shows that proper chemical composition promotes the oxidation of transition metal species and the chemisorption of OH-, thus accelerating the OER kinetics. Therefore, this work provides a hopeful method for designing and preparing transition metal phosphide/carbon composite as OER electrocatalysts.

5.
Int J Gen Med ; 15: 1381-1391, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35173475

RESUMO

AIM: The effect of sleep duration on cognitive function has been reported. However, the studies about the combined effects of total sleep duration and midday napping on cognition in elders were limited and inconclusive. We aimed to investigate the associations between total sleep duration, midday napping and cognitive function among middle-aged and older Chinese adults. METHODS: Based on the 3rd wave of the China Health and Retirement Longitudinal Study (CHARLS) in 2015, a total of 9218 participants aged ≥45 years with completed cognition measurements were included. Cognitive functions were assessed by a combined global cognition score of episodic memory and mental status. Information about sleep-related variables, demographic characteristics, and health status were collected by validated questionnaires. Multivariate linear regression models were performed to evaluate the associations between total sleep duration, midday napping, and cognitive function. Stratified analyses were used to explore the potential effect modifier. RESULTS: Overall, the global cognition score was 10.38 ± 4.30 among the participants (mean age: 61.5 ± 8.7 years). For sleep duration, both short sleep and long sleep duration were significantly associated with the increased risk of cognitive impairment after controlling for demographics and other confounders. Compared with sleeping for 7h per day, the adjusted ß-coefficient and 95% CI of the risk of cognitive impairment was -0.967 (95% CIs: -1.191, -0.742) for ≤5h, -0.257 (-0.498, -0.016) for 6h, -0.424 (-0.650, -0.198) for 8h and -0.664 (-0.876, -0.452) for ≥9h. The combined effect analysis indicated that subjects with extended or without naps had a significantly higher risk of cognitive impairment in the ≤5h sleep time group (ref: 7h) and in extended nappers or without naps group (ref: short nappers). Subjects with extended naps or without naps might increase the risk of cognitive impairment, especially those having ≤5h total sleep time. Stratified analysis showed that participants aged ≥60 years without social activity increased the risk of cognitive decline. CONCLUSION: An inverted U-shaped association was observed between total sleep duration and cognitive function in Chinese elders, especially in those aged ≥60 years or without social activity. Short midday naps could mitigate the deleterious effects of poor sleep quality and shorter sleep duration on cognitive function. The findings could help us identify the vulnerable population and decrease the burden of cognitive impairment.

6.
Adv Sci (Weinh) ; 9(11): e2103982, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35138033

RESUMO

Currently, the incidence of acute liver injury (ALI) is increasing year by year, and infection with coronavirus disease 2019 (COVID-19) can also induce ALI, but there are still no targeted therapeutic drugs. ZnO-NiO particles is mainly used to clean up reactive oxygen species (ROS) in industrial wastewater, and it is insoluble in water. Its excellent properties are discovered and improved by adding shuttle-based bonds to make it more water-soluble. ZnO-NiO@COOH particles are synthetically applied to treat ALI. The p-n junction in ZnO-NiO@COOH increases the surface area and active sites, thereby creating large numbers of oxygen vacancies, which can quickly adsorb ROS. The content in tissues and serum levels of L-glutathione (GSH) and the GSH/oxidized GSH ratio are measured to assess the capacity of ZnO-NiO@COOH particles to absorb ROS. The ZnO-NiO@COOH particles significantly reduce the expression levels of inflammatory factors (i.e., IL-1, IL-6, and TNF-α), macrophage infiltration, and granulocyte activation. ZnO-NiO@COOH rapidly adsorb ROS in a short period of time to block the generation of inflammatory storms and gain time for the follow-up treatment of ALI, which has important clinical significance.


Assuntos
COVID-19 , Óxido de Zinco , Glutationa , Humanos , Fígado , Níquel/química , Espécies Reativas de Oxigênio/metabolismo , Água , Óxido de Zinco/química
7.
J Colloid Interface Sci ; 613: 182-193, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35033764

RESUMO

Metal-organic-frameworks (MOFs) derived carbon or nitrogen-doped carbon (NC) materials are usually used as electromagnetic wave (EMW) absorbers. However, the effective control of the composition and structure of composites is still a major challenge for the development of high-performance EMW absorbing materials. In this work, core-shell structure and bimetallic composition Cu/nitrogen doped carbon @Co/ nitrogen doped carbon (Cu/NC@Co/NC) composites were designed and synthesized through the thermal decomposition of Cu-MOF@Co-MOF precursor. Cu/NC@Co/NC composites with different compositions were obtained by changing the ratio of Co-MOF and Cu-MOF. The composite (Cu/NC@Co/NC-3.75) prepared using 3.75 mmol of Co(NO3)2·6H2O exhibits outstanding EMW absorption properties due to the optimized impedance matching and strong attenuation ability, which is caused by enhanced interfacial and dipolar polarization as well as multiple reflection and scattering. With the filler loading in paraffin of 35 wt%, the minimum reflection loss (RLmin) is up to -54.13 dB at 9.84 GHz with a thin thickness of 3 mm, and the effective absorption bandwidth (EAB, RL≤ - 10 dB) reaches 5.19 GHz (10.18-15.37 GHz) with the corresponding thickness of 2.5 mm. Compared with the Cu/NC and Co/NC, the Cu/NC@Co/NC-3.75 composite exhibits much better EMW absorbing performances caused by the bimetallic composition and the unique core-shell structure. This work provides a rational design for MOF-derived lightweight and broadband EMW absorbing materials.

8.
Luminescence ; 37(2): 263-267, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34806291

RESUMO

Organophosphorus flame retardants (OPFRs) are new types of environmental pollutants, therefore the rapid and sensitive detection of OPFRs is a very important objective. A new experimental phenomenon was found in which tris(2-chloroethyl)phosphine (TCEP), a type of OPFR, could effectively enhance the signal of the luminol-H2 O2 chemiluminescence (CL) system. Combined with the controllability of flow injection analysis, a rapid, stable, and sensitive CL method was established. The CL intensity responded linearly to the concentration of TCEP in the range 0.5-100 µg/L (R2 = 0.999) with a low detection limit of 33 ng/L. Relative standard deviation (RSD) was 2.2% (n = 7, c = 100 µg/L). Water samples were labelled and recycled with RSDs of 1.1-5.7% and recoveries of 88.7-116.1%. Based on these results, this study established a new CL detection method for the environmental pollutant TCEP.


Assuntos
Retardadores de Chama , Luminescência , Luminol , Organofosfatos , Compostos Organofosforados , Fosfinas
9.
J Hum Genet ; 67(2): 107-114, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34462535

RESUMO

Infertility affects about 15% of heterosexual couples and male factors account for ~45-50% of clinical cases. Genetic factors play an important role in male infertility and thus we try to develop a cost-effective method for screening the genetic factors in male infertility. In our retrospective proof-of-concept study, we employed the high-throughput ligation-dependent probe amplification (HLPA) to examine the copy number by 115 genomic loci covering the Y chromosome, and 5 loci covering the X chromosome-specific region. We identified 8 sex chromosome aneuploid people from the low sperm concentration (LSC) group, and Y chromosome-specific microdeletion/duplications in 211 samples from the LSC group, and in 212 samples from the control group. 35 samples showed complete loss of AZFc (BPY2 to CDY1B deletion), which was not observed in controls. Nevertheless, a partial loss of AZFc (BPY2 to BPY2B deletion) was detected at comparable frequencies in both groups (68/211 vs. 108/212, respectively). And we further found structural variations in 28.6 and 26.9% samples from infertility and fertility groups. Moreover, we found that there were lower copy numbers for heterochromatic sequences in men with LSC. Especially, we reported that ultra-low relative copy number (RCN) (<0.5) type and low RCN (0.5 to <0.75) type in Yq12 were more often in the LSC group for the first time. Our results not only shed light on the potential role of low RCN in Yq12 in male infertility but also showed that HLPA can be a powerful and cost-effective tool for clinical screening in male infertility.


Assuntos
Cromossomos Humanos Y/genética , Variações do Número de Cópias de DNA/genética , Loci Gênicos/genética , Infertilidade Masculina/genética , Aberrações dos Cromossomos Sexuais , Proteínas de Ciclo Celular/genética , Humanos , Hibridização in Situ Fluorescente/métodos , Infertilidade Masculina/diagnóstico , Cariotipagem/métodos , Masculino , Reação em Cadeia da Polimerase Multiplex , Proteínas Nucleares/genética , Oligospermia/diagnóstico , Oligospermia/genética , Proteínas de Ligação a RNA/genética , Reprodutibilidade dos Testes , Estudos Retrospectivos , Sensibilidade e Especificidade , Contagem de Espermatozoides
10.
Sci Total Environ ; 812: 152371, 2022 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-34919930

RESUMO

Heatwaves with unprecedented conditions have devastating health impacts. The summer of 2017 saw unusual heat in China and other regions on earth. Although epidemiologic evidence is clear for elevated mortality risks of heatwaves, the economic impacts due to heatwave-associated mortality remain poorly characterized. Hence, this study systematically assessed the mortality and economic impacts of the 2017 exceptional heatwaves in China. We first used the generalized linear mixed-effect model with Poisson distribution to examine the mortality risks of the 2017 heatwaves in 91 Chinese counties. Further, we calculated the excess deaths attributable to heatwaves in 2852 counties. Finally, we evaluated the city- and province-level death-related economic burden of the 2017 heatwaves based on the value of statistical life (VSL). We found that the 2017 exceptional heatwaves had a statistically significant association (relative risk was 1.23, 95% confidence interval 1.14-1.32) with all-cause mortality across 91 Chinese counties. Nationwide, a total of 16,299 all-cause deaths that occurred in 2017 were attributable to the exceptional heatwaves, resulting in an overall death-related economic loss of 61,304 million RMB as valued by VSL. Given that extraordinary heatwaves are projected to be more frequent under global climate change, our findings could enhance the current understanding of heatwaves' health and economic impacts and add valuable insights in projection studies of estimating the future health burden of heatwaves.


Assuntos
Mudança Climática , Temperatura Alta , China/epidemiologia , Cidades , Humanos , Mortalidade , Estações do Ano
11.
Gene ; 809: 146022, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34673209

RESUMO

BACKGROUND: Genome-wide association studies have demonstrated that genetic variants are closely related to tumorigenesis and progression of cancer. However, the correlation between genetic variants in splicing factor genes and bladder cancer susceptibility remains unclear. METHOD: A case-control study with 580 cases of bladder cancer and 1,101 controls was conducted to explore the association of single-nucleotide polymorphisms (SNPs) in splicing factors with bladder cancer susceptibility by logistic regression models, and multiple testing errors were justified by the false discovery rate (FDR) method. Next, we used the Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) datasets to further analyze the differential expression of candidate genes. RESULTS: We found that rs978416 G>A in RBFOX3 contributed to a reduced risk of bladder cancer [adjusted odds ratio (OR) = 0.72, 95% confidence internal (CI) = 0.62-0.84, P = 3.54 × 10-5], especially in individuals who never smoked (P = 7.83 × 10-5). Stratified analysis showed that the protective effect of rs978416 was more significant in the subgroup of low grade and non-muscle invasive bladder cancer. Furthermore, the RBFOX3 mRNA expression was decreased in bladder tumor tissues. However, the relatively high expression of RBFOX3 was related to a higher bladder cancer stage. CONCLUSIONS: Our findings indicated that SNP rs978416 G>A in RBFOX3 may be related to bladder cancer predisposition in Chinese population and might serve as a novel biomarker for bladder cancer risk.


Assuntos
Antígenos Nucleares/genética , Proteínas do Tecido Nervoso/genética , Polimorfismo de Nucleotídeo Único , Fatores de Processamento de RNA/genética , Neoplasias da Bexiga Urinária/genética , /genética , Biomarcadores Tumorais/genética , Estudos de Casos e Controles , Regulação Neoplásica da Expressão Gênica , Predisposição Genética para Doença , Humanos , Neoplasias da Bexiga Urinária/patologia
12.
Molecules ; 26(23)2021 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-34885838

RESUMO

Prunus mume is a traditional ornamental plant, which owed a unique floral scent. However, the diversity of the floral scent in P. mume cultivars with different aroma types was not identified. In this study, the floral scent of eight P. mume cultivars was studied using headspace solid-phase microextraction (HS-SPME) and organic solvent extraction (OSE), combined with gas chromatography-mass spectrometry (GC-MS). In total, 66 headspace volatiles and 74 endogenous extracts were putatively identified, of which phenylpropanoids/benzenoids were the main volatile organic compounds categories. As a result of GC-MS analysis, benzyl acetate (1.55-61.26%), eugenol (0.87-6.03%), benzaldehyde (5.34-46.46%), benzyl alcohol (5.13-57.13%), chavicol (0-5.46%), and cinnamyl alcohol (0-6.49%) were considered to be the main components in most varieties. However, the volatilization rate of these main components was different. Based on the variable importance in projection (VIP) values in the orthogonal partial least-squares discriminate analysis (OPLS-DA), differential components of four aroma types were identified as biomarkers, and 10 volatile and 12 endogenous biomarkers were screened out, respectively. The odor activity value (OAV) revealed that several biomarkers, including (Z)-2-hexen-1-ol, pentyl acetate, (E)-cinnamaldehyde, methyl salicylate, cinnamyl alcohol, and benzoyl cyanide, contributed greatly to the strong-scented, fresh-scented, sweet-scented, and light-scented types of P. mume cultivars. This study provided a theoretical basis for the floral scent evaluation and breeding of P. mume cultivars.


Assuntos
Odorantes/análise , Extratos Vegetais/análise , Prunus/química , Compostos Orgânicos Voláteis/análise , Biomarcadores/análise , Análise Discriminante , Flores/química , Análise dos Mínimos Quadrados , Volatilização
13.
Int J Gen Med ; 14: 8689-8710, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34849012

RESUMO

INTRODUCTION: As a highly aggressive tumor with a poor prognosis, esophageal cancer (ESCA)'s relationship with gene mutations is unclear. Therefore, we tried to explore the role of gene mutation in ESCA progression and its relationship with immune response, clinical treatment, and prognosis. METHODS: In addition to copy number variation (CNV) situations of common genes obtained from 2 public databases, the relationship between mutations and prognosis/tumor mutational burden (TMB) was also analyzed. Kaplan-Meier survival and Cox regression analysis were used to identify the CSMD1 mutation status as an independent predictor of prognosis. We also enriched related functions and pathways. Next, the relationship between 22 immune cells and CSMD1 mutation status was analyzed. In addition to the differences in the expression levels of immune checkpoint inhibitors (ICIs)-related genes between the high TMB and low TMB groups, the differences in the expression levels of ICIs/m6a/multi-drug resistance-related genes and the sensitivity of three chemotherapeutic drugs between CSMD1 mutant and the wild group were also compared. In addition to differences and prognostic analysis of CSMD1 expression, the correlation analysis between the expression of these genes/immune cells and the expression of CSMD1 was also performed. Finally, a nomogram that could efficiently and conveniently predict the survival probability of ESCA patients was constructed and verified. RESULTS: We obtained 17 frequently mutated genes distribution. Mutation and loss of CSMD1 are frequent in ESCA. Only CSMD1 mutation can be used as an independent predictor of poor prognosis. Patients in the high TMB group have a lower survival probability. Wild CSMD1 may be involved in immune-related pathways. More helper T cells and fewer resting state dendritic cells were found in the CSMD1 mutant group. The PD-1 expression in the high TMB group showed higher. Paclitaxel sensitivity and ABCC1 expression were higher in the wild CSMD1 group. Most cancers show differential expression of CSMD1. Except for the prognosis of ESCA, the expression of CSMD1 is related to immune cell content and the expression of ICIs/m6a/multi-drug resistance related genes. DISCUSSION: CSMD1 mutation could be used as an immune-related biomarker to predict prognosis and treatment effect of paclitaxel. Mutation and loss of CSMD1 may promote the progression of ESCA.

14.
Adv Sci (Weinh) ; 8(24): e2102545, 2021 12.
Artigo em Inglês | MEDLINE | ID: mdl-34719880

RESUMO

Damaged skin cannot prevent harmful bacteria from invading tissues, causing infected wounds and even serious tissue damage. Traditional treatments can not only kill pathogenic bacteria, but also suppress the growth of beneficial bacteria, thus destroying the balance of the damaged skin microbial ecosystem. Here, a living bacterial hydrogel scaffold is reported that accelerates infected wound healing through beneficial bacteria secreting antibacterial substances. Lactobacillus reuteri, a common probiotic, is encapsulated in hydrogel microspheres by emulsion polymerization and further immobilized in a hydrogel network by covalent cross-linking of methacrylate-modified hyaluronic acid. Owing to light-initiated crosslinking, the hydrogel dressing can be generated in situ at the wound site. This hydrogel scaffold not only protects bacteria from immune system attack, but also prevents bacteria from escaping into the local environment, thus avoiding potential threats. Both in vitro and in vivo experiments show that it has excellent ability against harmful bacteria and anti-inflammatory capabilities, promoting infected wound closure and new tissue regeneration. This work may open up new avenues for the application of living bacteria in the clinical management of infected wounds.


Assuntos
Antibacterianos/uso terapêutico , Hidrogéis/uso terapêutico , Cicatrização/fisiologia , Infecção dos Ferimentos/terapia , Animais , Antibacterianos/administração & dosagem , Modelos Animais de Doenças , Camundongos
15.
China CDC Wkly ; 3(4): 61-64, 2021 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-34595003

RESUMO

SUMMARY: What is already known about this topic? While the establishment of an air quality health index (AQHI) in some countries yielded positive outcomes in communicating health risks of air pollution, China lagged behind in developing its own AQHI. Several research studies of AQHI were conducted in China, but this scientific research has not yet been applied to standards. What is added by this report? This report introduced the method of calculation of Chinese AQHI to be launched in pilot cities. The index in this report was established on the basis of fully drawing on international experience and considering Chinese characteristics. What are the implications for public health practice? The purpose of this report is to guide unified application of the AQHI throughout China and translate scientific evidence into public services to promote public health. Based on the AQHI construction method in this report, an AQHI real-time computing platform and data transfer interface will be developed. The release of AQHI aims to communicate health risk of air pollution and provide scientific health protective guidance to the general public, accordingly to protect people's health.

16.
Risk Manag Healthc Policy ; 14: 4199-4209, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34675714

RESUMO

BACKGROUND: Severe acute respiratory syndrome (SARS) was reported first in China in 2003. The world is currently coping with coronavirus disease-2019 (COVID-19). We conducted a retrospective study to compare the initial public-health emergency response (PHER) to SARS and COVID-19 in mainland China. METHODS: A qualitative comparative study was conducted to compare the PHER timelines to SARS and COVID-19 by selecting six crucial time points. Besides, we explored the speed of spread, peak time and plateau period of SARS and COVID-19, respectively, by comparing the confirmed cases in the same time interval. RESULTS: The government of the People's Republic of China (PRC) accomplished the entire initial PHER to SARS in 127 days and for COVID-19 in 44 days. The speed of PHER for COVID-19 was 83 days faster. The peak time of SARS arose ~80 days later than that of COVID-19. Though the peak number of confirmed daily cases for COVID-19 was fivefold more than that of SARS, the onset of the stabilization period for COVID-19 was >2 months earlier than that of SARS. CONCLUSION: Overall, the speed of the initial PHER to COVID-19 pandemic was faster than that for SARS. Compared with the speed of hospital reporting and government policymaking, the speed of pathogen identification improved the most. The COVID-19 pandemic curve entered a plateau period earlier than the SARS pandemic curve, which suggests that the pandemic was controlled more effectively because of a timely PHER. The PRC government should emphasize improving the ability of hospitals to restrain infectious diseases by enhancing the direct reporting system and cultivating crisis management to empower relevant individuals to make timely scientific decisions.

17.
EBioMedicine ; 72: 103628, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34653870

RESUMO

A disintegrin and metalloproteinases (ADAMs) family are proteolytic transmembrane proteases that modulate diverse cell functions and coordinate intercellular communication. ADAMs are responsible for regulating cell proliferation, differentiation, migration, and organ morphogenesis in kidney development. Abnormally activated ADAMs drive inflammation and fibrosis in response to kidney diseases such as acute kidney injury, diabetic kidney disease, polycystic kidney disease, and chronic allograft nephropathy. ADAM10 and ADAM17, known as the most characterized members of ADAMs, are extensively investigated in kidney diseases. Notably, ADAM proteases have the potential to be targets for developing novel treatment approaches in kidney diseases.


Assuntos
Proteínas ADAM/metabolismo , Nefropatias/metabolismo , Rim/metabolismo , Animais , Comunicação Celular/fisiologia , Humanos , Inflamação/metabolismo
18.
Artigo em Inglês | MEDLINE | ID: mdl-34639264

RESUMO

The spatial accessibility of prehospital EMS is particularly important for the elderly population's physiological functions. Due to the recent expansion of aging populations all over the globe, elderly people's spatial accessibility to prehospital EMS presents a serious challenge. An efficient strategy to address this issue involves using geographic information systems (GIS)-based tools to evaluate the spatial accessibility in conjunction with the spatial distribution of aging people, available road networks, and prehospital EMS facilities. This study employed gravity model and empirical Bayesian Kriging (EBK) interpolation analysis to evaluate the elderly's spatial access to prehospital EMS in Ningbo, China. In our study, we aimed to solve the following specific research questions: In the study area, "what are the characteristics of the prehospital EMS demand of the elderly?" "Do the elderly have equal and convenient spatial access to prehospital EMS?" and "How can we satisfy the prehospital EMS demand of an aging population, improve their spatial access to prehospital EMS, and then ensure their quality of life?" The results showed that 37.44% of patients admitted to prehospital EMS in 2020 were 65 years and older. The rate of utilization of ambulance services by the elderly was 27.39 per 1000 elderly residents. Ambulance use by the elderly was the highest in the winter months and the lowest in the spring months (25.90% vs. 22.38%). As for the disease spectrum, the main disease was found to be trauma and intoxication (23.70%). The mean accessibility score was only 1.43 and nearly 70% of demand points had scored lower than 1. The elderly's spatial accessibility to prehospital EMS had a central-outward gradient decreasing trend from the central region to the southeast and southwest of the study area. Our proposed methodology and its spatial equilibrium results could be taken as a benchmark of prehospital care capacity and help inform authorities' efforts to develop efficient, aging-focused spatial accessibility plans.


Assuntos
Serviços Médicos de Emergência , Qualidade de Vida , Idoso , Ambulâncias , Teorema de Bayes , China , Humanos
19.
iScience ; 24(10): 103186, 2021 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-34608450

RESUMO

The COVID-19 pandemic has caused over 220 million infections and 4.5 million deaths worldwide. Current risk factor cannot fully explain the diversity in disease severity. Here, we present a comprehensive analysis of a broad range of patients' laboratory and clinical assessments to investigate the genetic contributions to COVID-19 severity. By performing GWAS analysis, we discovered several concrete associations for laboratory traits and used Mendelian randomization (MR) analysis to further investigate the causality of traits on disease severity. Two causal traits, WBC counts and cholesterol levels, were identified based on MR study, and their functional genes are located at genes MHC complex and ApoE, respectively. Our gene-based analysis and GSEA revealed four interferon pathways, including type I interferon receptor binding and SARS coronavirus and innate immunity. We hope that our work will contribute to studying the genetic mechanisms of disease and serve as a useful reference for COVID-19 diagnosis and treatment.

20.
Biomater Sci ; 9(16): 5577-5587, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34241605

RESUMO

Colon cancer is one of the most common cancers with high mortality, and can easily spread and metastasize, remaining an urgent disease to be solved. Nanomaterial-associated starvation or photothermal therapy has been considered to be a promising strategy in tumor therapy. However, the therapeutic effect of a single regimen for cancer treatment still needs to be improved due to their respective limitations. Herein, a biomimetic multifunctional nanoreactor is developed by encapsulating glucose oxidase and gold nanorods (AuNRs) in an erythrocyte membrane camouflaged metal-organic framework (MOF) nanoparticle (ZGAM), which was exploited for synergistic treatment of colon cancer by combining glucose oxidase-based starvation with AuNR-based photothermal therapy (PTT). This biomimetic nanoreactor could not only exhaust endogenous glucose to suppress the growth of the tumor by the released glucose oxidase (GOx), but also enhance the effect of photothermal therapy via inhibiting the expression of heat shock protein (HSP). In vitro and in vivo investigations indicate that this biomimetic nanoreactor shows excellent therapeutic effects on tumors, resulting from the synergistic treatment of starvation therapy and PTT. Therefore, the proposed strategy may open a window to develop an intelligent therapeutic system for better therapeutic efficacy against cancer.


Assuntos
Neoplasias do Colo , Estruturas Metalorgânicas , Nanopartículas , Neoplasias , Linhagem Celular Tumoral , Neoplasias do Colo/terapia , Glucose Oxidase , Humanos , Neoplasias/terapia , Terapia Fototérmica
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