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1.
Water Sci Technol ; 82(11): 2234-2249, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33339780

RESUMO

This study aimed to develop a novel composite membrane based on polyethersulfone (PES) and modified activated carbon fibers (ACFs) to remove of sulfamethoxazole (SMZ) from water. The modification of ACFs was conducted by using acid, Fe, and Mn and was confirmed by Fourier transform infrared spectroscopy (FT-IR), energy dispersive X-ray spectroscopy (EDS), and water contact angle measurement. Later on, the composite membranes were prepared using PES (9 wt%), N-N-dimethylacetamide (DMAc) (75 wt%), polyethylene pyrrolidone (PVP) (5 wt%), anhydrous lithium chloride (LiCl) (1 wt%), and various types of modified ACFs (0.8 wt%) as additives. It was found that the contact angle of the membrane decreased by more than 20°, and the zeta potential decreased by more than 10 mV. ACF modified by Fe was used as an admixture, membrane obtained the high comprehensive performance. Especially bovine serum albumin (BSA) rejection rate and flux recovery ratio (FRR) reached 98.8% and 98.4%, respectively. And the removal rates of SMZ increased by 24.6% under the electric field. The degradation products were detected by high-performance liquid chromatography/mass spectrometry (HPLC/MS). Based on this result, the possible degradation pathways of SMZ are proposed.


Assuntos
Carvão Vegetal , Água , Fibra de Carbono , Membranas Artificiais , Polímeros , Espectroscopia de Infravermelho com Transformada de Fourier , Sulfonas
2.
BMC Musculoskelet Disord ; 21(1): 774, 2020 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-33234145

RESUMO

BACKGROUND: In the management of early inflammatory joint of ankylosing spondylitis (AS), there is a need for reliable noninvasive quantitative monitoring biomarker to closely assess status of synovitis progression. Cognizant to this,studies geared on improving techniques for quantitative evaluation of micro-environment permeability of the joint space are necessary. Such improved techniques may provide tissue perfusion as important biological parameters and can further help in understanding the origin of early changes associated with AS. The purpose of this study was to prospectively evaluate the diagnostic performance and determine longitudinal relationships of early micro-environment active in the joint space of the sacroiliac joint (SIJ) with a rat model by using quantitative dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). METHODS: Thirty wistar male rats were randomly assigned to the model (n = 15) or control (n = 15) group. All rats underwent DCE-MRI of SIJ region at fixed time points (12, 17 and 22 weeks),between September 2018 and October 2019. Differences in permeability parameters between the two groups at the same time point were compared by using an independent samples t test. Spearman correlations of DCE-MRI parameters with different time points in model group were analyzed. All statistical analyses were performed with software. RESULTS: At 12 weeks,the Ktrans,Kep and Ve values in the model group were slightly lower than those in control group,but all the differences were not statistically significant (p > 0.05). Compared with control group,the transfer constant (Ktrans) values increased significantly at 17 weeks and 22 weeks in model group,while the rate constant (Kep) and volume of extravascular extracellular space (Ve) significantly increased only at 22 weeks(p < 0.05). The Ktrans,Kep and Ve were positively correlated with increasing time points (r = 0.946, P<0.01 for Ktrans; r = 0.945, P<0.01 for Kep; and r = 0.832, P<0.01 for Ve). CONCLUSION: Quantitative DCE-MRI parameters are valuable for evaluating the early longitudinal relationship of micro-environment permeability changes in the joint space of SIJ.

3.
Vaccines (Basel) ; 8(3)2020 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-32899579

RESUMO

Respiratory infections are still a major concern in pigs. Amongst the involved viruses, the porcine reproductive and respiratory syndrome virus (PRRSV) and the swine influenza type A virus (swIAV) have a major impact. These viruses frequently encounter and dual infections are reported. We analyzed here the molecular interactions between viruses and porcine tracheal epithelial cells as well as lung tissue. PRRSV-1 species do not infect porcine respiratory epithelial cells. However, PRRSV-1, when inoculated simultaneously or shortly before swIAV, was able to inhibit swIAV H1N2 infection, modulate the interferon response and alter signaling protein phosphorylations (ERK, AKT, AMPK, and JAK2), in our conditions. SwIAV inhibition was also observed, although at a lower level, by inactivated PRRSV-1, whereas acid wash treatment inactivating non-penetrated viruses suppressed the interference effect. PRRSV-1 and swIAV may interact at several stages, before their attachment to the cells, when they attach to their receptors, and later on. In conclusion, we showed for the first time that PRRSV can alter the relation between swIAV and its main target cells, opening the doors to further studies on the interplay between viruses. Consequences of these peculiar interactions on viral infections and vaccinations using modified live vaccines require further investigations.

5.
Front Immunol ; 11: 1669, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32922386

RESUMO

The innate immunity DNA sensors have drawn much attention due to their significant importance against the infections with DNA viruses and intracellular bacteria. Among the multiple DNA sensors, IFI16, and cGAS are the two major ones, subjected to extensive studies. However, these two DNA sensors in livestock animals have not been well defined. Here, we studied the porcine IFI16 and cGAS, and their mutual relationship. We found that both enable STING-dependent signaling to downstream IFN upon DNA transfection and HSV-1 infection, and cGAS plays a major role in DNA signaling. In terms of their relationship, IFI16 appeared to interfere with cGAS signaling as deduced from both transfected and knockout cells. Mechanistically, IFI16 competitively binds with agonist DNA and signaling adaptor STING and thereby influences second messenger cGAMP production and downstream gene transcription. Furthermore, the HIN2 domain of porcine IFI16 harbored most of its activity and mediated cGAS inhibition. Thus, this study provides a unique insight into the porcine DNA sensing system.

6.
Transbound Emerg Dis ; 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32931644

RESUMO

To date, four species of porcine circoviruses (PCVs), including PCV1-4, have been reported to exist in the clinical cases. Fast and effective differential detection is critical to monitor the infection and co-infection status of PCVs for adopting reliable control strategies. However, currently available methods cannot simultaneously differentiate the four species of PCV strains. In this study, a quadruplex real-time PCR assay based on TaqMan probes was developed for differential detection of PCV1-4. The new quadruplex real-time PCR assay exhibited satisfied specificity, sensitivity, repeatability and reproducibility. In addition, the new assay was applied to the detection of 120 clinical samples collected from 2016 to 2020 in Jiangsu province of China and compared with previously reported PCV1-4 singleplex conventional PCR assays. Based on the clinical performance, the results from the quadruplex real-time PCR and conventional PCR assays showed 100% agreement. A total of 47 samples were detected as PCV positive by the quadruplex real-time PCR assay, including 1, 2, 1 samples were co-infected with PCV1 and PCV4, PCV2 and PCV3, PCV2 and PCV4, respectively. Full-length ORF2 sequencing and phylogenetic analysis supported the real-time PCR results that 5, 34, 8 and 4 of the 51 PCV sequences were PCV1, PCV2, PCV3 and PCV4, respectively. This study provides a promising alternative tool for rapid differential detection of PCVs and confirms the coexistence of all species of PCV1-4 strains in Jiangsu province in recent years.

7.
Vaccines (Basel) ; 8(3)2020 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-32660114

RESUMO

The innate DNA sensing receptors are one family of pattern recognition receptors and play important roles in antiviral infections, especially DNA viral infections. Among the multiple DNA sensors, cGAS has been studied intensively and is most defined in mammals. However, DNA sensors in chickens have not been much studied, and the chicken cGAS is still not fully understood. In this study, we investigated the chicken cGAS-STING signal axis, revealed its synergistic activity, species-specificity, and the signal essential sites in cGAS. Importantly, both cGAS and STING exhibited antiviral effects against DNA viruses, retroviruses, and RNA viruses, suggesting the broad range antiviral functions and the critical roles in chicken innate immunity.

8.
BMC Musculoskelet Disord ; 21(1): 472, 2020 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-32689978

RESUMO

BACKGROUND: Currently, few studies have described the relationship between functional MRI findings and histology of normal sacroiliac joint (SIJ). Besides, due to the difficulties in access to SIJ, authentic animal models are important in providing opportunities for quantitative parameter extraction on imaging. AIMS: This study aimed at exploring the parameters of Intravoxel Incoherent Motion Diffusion-Weighted Imaging (IVIM-DWI) and Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) and comparing them with the histology of the SIJ in normal rats with different ages. METHODS: A total of thirty 7-week-old male Wistar rats were included in the study. The parameters of IVIM-DWI and DCE-MRI in the bone marrow and the joint space of SIJ were measured at 8, 13, 18, 23, 28, and 33 weeks. The histological analysis of the SIJ was examined using light microscopy. One-way ANOVA was used for statistical analysis. RESULTS: The D values in the sacral and iliac bone marrow of normal rats decreased with an increase in age. One-way ANOVA analysis indicated a significant difference in D values in different age groups (P<0.005). The normal values of D*, f, Fenh (%), Senh (%/s) in the sacral bone marrow, the iliac bone marrow, and the joint space in SIJ of normal rats were obtained. The results showed that in the six groups of rats of different ages, the histology of the SIJ surface was smooth and clear, the cartilage cells were intact, and no thickening or pannus formation was observed. CONCLUSIONS: This study obtained the IVIM-DWI and DCE-MRI parameters of the sacral and iliac bone marrow and the synovial area of the joint space in normal rats. The parameters in normal rats can be used in future research to compare to similar parameters in animal models or patients with SIJ diseases. This study serves as a guide for future research in SIJ diseases.

9.
Vet Res ; 51(1): 80, 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32546263

RESUMO

Understudied, coinfections are more frequent in pig farms than single infections. In pigs, the term "Porcine Respiratory Disease Complex" (PRDC) is often used to describe coinfections involving viruses such as swine Influenza A Virus (swIAV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and Porcine CircoVirus type 2 (PCV2) as well as bacteria like Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae and Bordetella bronchiseptica. The clinical outcome of the various coinfection or superinfection situations is usually assessed in the studies while in most of cases there is no clear elucidation of the fine mechanisms shaping the complex interactions occurring between microorganisms. In this comprehensive review, we aimed at identifying the studies dealing with coinfections or superinfections in the pig respiratory tract and at presenting the interactions between pathogens and, when possible, the mechanisms controlling them. Coinfections and superinfections involving viruses and bacteria were considered while research articles including protozoan and fungi were excluded. We discuss the main limitations complicating the interpretation of coinfection/superinfection studies, and the high potential perspectives in this fascinating research field, which is expecting to gain more and more interest in the next years for the obvious benefit of animal health.

10.
Clin Imaging ; 68: 226-231, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32425337

RESUMO

Objective: To retrospectively analyze the CT findings in patients infected with Coronavirus disease 2019 (COVID-19). Materials and methods: The thirty-four cases, 15 females and 19 males, with an age ranging from 7 to 88 years old, confirmed by real-time reverse-transcriptase-polymerase chain reaction (RT-PCR), were used for our study. All thin-section CT scans of the lungs were performed in all of patients. The clinical, laboratory and CT imaging were available to evaluate in all patients. Results: The patients present with fever (85.29%, n = 29), cough (67.65%, n = 23), fatigue or myalgia (26.47%, n = 9), and pharyngalgia (8.82%, n = 3). The 4 patients (11.76%) with no symptoms were identified during screening for close contacts, who had typical CT findings. On initial CT scans, the bilateral lung involved was shown in 24 cases (70.59%), while 29 (82.35%) cases were distributed in peripheral. The pure ground glass opacity (GGO) was shown in 18 cases (52.94%), the GGO with consolidation was in 12 cases (35.29%), and full consolidation only in 3 cases. The lesion with air bronchogram was seen in 14 (41.18%) cases, with enlarged blood vessel in 17 (50.00%) cases, with crazy-paving pattern in 8 (23.53%) cases, with fine reticular pattern in 4 (11.77%) cases, and with intralesional vacuole sign in 6 (17.65%) cases. The pleural effusion was seen in one patient. Follow-up imaging in 19 patients during the study time window demonstrated mild, moderate or severe progression of disease, as manifested by increasing extent and density of lung opacities. Conclusions: The bilateral GGO with air bronchogram, enlarged blood vessel, fine reticular pattern, and peripheral distribution are the early CT findings of COVID-19. The crazy-paving pattern and intralesional vacuole sign are the features of progressive stage.


Assuntos
Infecções por Coronavirus/patologia , Pulmão/patologia , Pneumonia Viral/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Betacoronavirus , Vasos Sanguíneos/patologia , Criança , Coronavirus , Infecções por Coronavirus/complicações , Infecções por Coronavirus/diagnóstico por imagem , Infecções por Coronavirus/virologia , Tosse/etiologia , Feminino , Humanos , Hipertrofia , Pulmão/diagnóstico por imagem , Pulmão/virologia , Masculino , Pessoa de Meia-Idade , Pandemias , Derrame Pleural/etiologia , Pneumonia Viral/complicações , Pneumonia Viral/diagnóstico por imagem , Pneumonia Viral/virologia , Estudos Retrospectivos , Tomografia Computadorizada por Raios X/métodos , Adulto Jovem
11.
Transbound Emerg Dis ; 2020 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-32304348

RESUMO

Multiple commercial porcine reproductive and respiratory syndrome (PRRS) modified live vaccines are currently utilized in Chinese swine herds due to the limited cross-protection of vaccines and coexistence of different PRRS viruses. In this study, an infectious cDNA clone of the highly pathogenic PRRS (HP-PRRS) vaccine JXA1-R strain was generated. We successfully rescued the virus from direct in vitro DNA transfection of rJXA1-R clone, which has similar growth kinetics to the parental JXA1-R virus in Marc-145 cells. To further evaluate the potential use of the cloned rJXA1-R virus as a live vector for foreign gene expression, the enhanced green fluorescent protein (EGFP) was inserted between non-structural and structural genes. Our results showed that the dynamic expression of EGFP can be visualized by live cell imaging system during the infection in Marc-145 cells. The availability of our cloned JXA1-R viruses provides a crucial platform to study the fundamental biology of HP-PRRS virus vaccine and also serves as a potential effective vector for developing live vector vaccines against swine pathogens.

12.
Res Vet Sci ; 131: 38-42, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32289611

RESUMO

High genetic diversity and limited cross-protection are two major reasons for ineffective control of porcine reproductive and respiratory syndrome virus (PRRSV) infection. Therefore, it's important to dynamically monitor the prevalence of PRRSV for adopting appropriate control strategy. In this study, we analyzed PRRSV infection by detecting 712 clinical samples collected from 2016 to 2019 in China. Totally 100 samples were detected as PRRSV positive, including 2 and 98 samples were infected with PRRSV1 and PRRSV2, respectively. In addition, two out of the 98 PRRSV2 positive samples were co-infected with two distinct viruses. ORF5-based phylogenetic analysis showed that JXA1-like HP-PRRSV2 (lineage 8) and NADC30-like PRRSV2 (lineage 1) isolates are currently predominant, but QYYZ-like PRRSV2, CH-1a-like PRRSV2 and PRRSV1 isolates also co-exist in Chinese swine herds. In addition, two commercial MLV-derived viruses (TJM-F92-like and JXA1-R-like) were frequently detected. GP5 alignment also detected insertion and deletion in the extravirion domain. Our study presents the up-to-date PRRSV infection status and highlights the high genetic diversity of PRRSV currently circulating in China.


Assuntos
Variação Genética , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Animais , China/epidemiologia , Filogenia , Síndrome Respiratória e Reprodutiva Suína/epidemiologia , Deleção de Sequência , Suínos
13.
Mol Immunol ; 121: 20-27, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32142955

RESUMO

To study the interrelationship between the signaling adaptors of innate pattern recognition receptor (PRR) pathways including toll-like receptor (TLR), retinoic acid-inducible gene-1-like receptor (RLR), nucleotide-binding oligomerization domain-like receptor (NLR), and cytoplasmic DNA recognition receptors (CDR) pathways. The coding genes of porcine TRIF, MAVS, STING, MyD88, RIPK2, and ASC were isolated from PK15 cells. Phylogenetic analysis of the six adaptor proteins in pig, cattle, goat, horse, human, mouse, chicken, and duck performed by MEGA 5.05 showed that these adaptors have slightly different similarity across species. The expression of these proteins in transfected cells were detected by both Western blotting and confocal microscopy. All six adaptors were visualized in cytoplasm but with different distribution patterns. The activities of the six adaptors triggering NF-κB and ISRE signaling and downstream gene productions were examined by dual-luciferase reporter assay and real-time RT-PCR, respectively. The results showed that STING has an ability to activate ISRE signaling, MyD88, RIPK2 and ASC possess NF-κB signal activity, while TRIF and MAVS can activate both. Furthermore, the mutual signaling effects were assessed by NF-κB and ISRE dual-luciferase reporter assay in the co-expression experiments. STING was shown to enhance MAVS activated NF-κB signaling and MyD88 could heighten STING activated ISRE signaling. However, all other adaptors inhibited each other to varying degrees. The work provides a global insight of porcine innate immune signaling pathways and their interaction network.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Imunidade Inata , Receptores de Reconhecimento de Padrão/metabolismo , Transdução de Sinais/imunologia , Proteínas Adaptadoras de Transdução de Sinal/genética , Proteínas Adaptadoras de Transdução de Sinal/imunologia , Proteínas Adaptadoras de Transdução de Sinal/isolamento & purificação , Animais , Células HEK293 , Humanos , Filogenia , Receptores de Reconhecimento de Padrão/imunologia , Suínos
14.
Magn Reson Imaging ; 68: 30-35, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-31978516

RESUMO

OBJECTIVE: To prospectively evaluate the ability of IVIM-DWI and DCE-MRI in detecting early activity of sacroiliitis in rat model of ankylosing spondylitis by comparing with pathological results. METHODS: 20 wistar male rats were induced by bovine proteoglycan combined with complete/incomplete Freund's adjuvant as model group, and 20 healthy male rats were used as the control group. The parameters of IVIM-DWI and DCE-MRI in synovial regions of SIJ were measured respectively at 7th, 12th, 17th, and 22th weeks after the last induction, and the pathological features of SIJ were taken also, further studying the pathological characteristics of sacroiliac region. Independent sample t-test and one-way ANOVA were used for statistical analysis. The prediction parameters and diagnostic efficiency were compared by ROC curve. RESULTS: There was no significant difference of image parameters between the model and control groups at the 7th, 12th weeks after the last induction, and there were no positive findings in histopathological examination at the same time. At the 17th week after induction, the f and Fenh%, Senh% between the model and the control groups were statistically significant. At the 22th week, there was a statistically significant increase all the values in model group than those in control group (P < 0.05). Histologic examination confirmed inflmmtorycell infiitrtion at the 17th week and pannus forming of synovium on the surface of cartilage at the 22th week in the model groups. The Fenh%, Senh%, Dslow and f had the moderate diagnostic efficiency and the areas under the curve were 0.77, 0.75, 0.77 and 0.82 respectively. The Senh% demonstrated the highest sensitivity (71.4%) and f demonstrated the highest specificity (95.0%). CONCLUSION: IVIM-DWI and DCE-MRI can be used as the sensitive imaging methods to detect and accurate diagnosis the early activity of sacroiliitis in AS.


Assuntos
Meios de Contraste , Processamento de Imagem Assistida por Computador/métodos , Sacroileíte/diagnóstico por imagem , Espondilite Anquilosante/diagnóstico por imagem , Análise de Variância , Animais , Área Sob a Curva , Bovinos , Imagem de Difusão por Ressonância Magnética , Modelos Animais de Doenças , Masculino , Movimento (Física) , Variações Dependentes do Observador , Proteoglicanas/química , Curva ROC , Ratos , Ratos Wistar , Sensibilidade e Especificidade
15.
Crit Rev Food Sci Nutr ; 60(4): 695-708, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-30638035

RESUMO

The objective of this review is to summarize knowledge on the inhibitory effects (IEs) of flavonoids on α-amylase (αA) and α-glucosidase (αG) relevant to the search of substitutes of acarbose (Aca). Flavonoids reported to be more effective at inhibiting αG than Aca have been summarized. The concept of "relative coefficient to Aca (RCAca)" has been proposed to integrate data from various reports. Correlations between hydrogen bond donors (H-donors), hydrogen bond acceptors (H-acceptors), partition coefficient values (XLog P3), and RCAca are discussed. Two kinds of binding modes between flavonoids and enzymes have been observed: (i) flavonoids directly bind to amino acid residues (AARs) in the active sites of enzymes and exclude the binding of substrate; (ii) flavonoids interact with AARs near the active site and close the channel to the active center. Some groups are correlated with stronger IEs: (i) substitutions of caffeoyl, galloyl, and prenyl groups in flavonoids enhance IEs; (ii) steric hindrance attenuates IEs, and linear molecules tend to be stronger inhibitors of porcine pancreatic αA (PPA). Whilst many achievements have been made, our understanding of the combined effects of different flavonoids, and flavonoids and Aca, remain ambiguous, and the effects of food matrices and stomach digestion on IEs of flavonoids are poorly understood. This review provides a comprehensive understanding on the use of flavonoids as αA and αG inhibitors for controlling diabetes.


Assuntos
Flavonoides/farmacologia , Inibidores de Glicosídeo Hidrolases/farmacologia , alfa-Amilases/antagonistas & inibidores , alfa-Glucosidases/metabolismo , Acarbose/farmacologia , Animais , Diabetes Mellitus/tratamento farmacológico , Diabetes Mellitus/enzimologia , Humanos
16.
Dev Comp Immunol ; 104: 103555, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-31751629

RESUMO

As one family of pattern recognition receptors (PRRs), The C-type lectin receptors (CLRs) play a key role in the anti-fungal infection. The CLR pathway signaling is relayed by adaptor complex which comprises CARD9, BCL10 and MALT1. However, the relationship between these three adaptors has not been investigated. In this study, we isolated porcine CARD9, BCL10 and MALT1 and examined their signaling functions. The three ectopic adaptors were similarly and uniformly expressed in cytoplasm, with CARD9 inactive, BCL10 significant active, and MALT1 slightly active for downstream NF-κB signaling and gene expressions. With the three adaptors together, NF-κB signaling and gene expressions were strongly activated, however, no IFN signal was activated in any case. The signaling relationship between the adaptors were dissected, the NF-κB signaling results showed that CARD9 could inhibit both BCL10 and MALT1 activities, while BCL10 and MALT1 synergized each other particularly when moderate amount of BCL10 plus low amount of MALT1 were considered. Low amount of CARD9 could further synergized with BCL10 and MALT1, maximizing signaling activity of the adaptor complex. This study revealed the porcine CLR pathway adaptor signaling functions and their optimal collective activity, thus providing a unique insight into the porcine innate immunity.

17.
Clin Lab ; 65(11)2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31710444

RESUMO

BACKGROUND: The current study aims to explore the clinical value of serum miR-499a-5p in the diagnosis of pancreatic cancer. METHODS: One hundred twenty-four patients with pancreatic cancer (cancer group), 100 patients with benign pancreatic diseases (benign control group), and 100 healthy people (healthy control group) were selected as the observation objects from January 2017 to June 2017. Fasting venous blood samples were collected to detect the levels of CA199 and the relative expression of miR-499a-5p in serum, and to evaluate the diagnostic value for pancreatic cancer. RESULTS: The expression of CA199 in the benign control group and cancer group was significantly higher than that in the healthy control group. However, the expression of miR-499a-5p in the cancer group was significantly higher than that in the benign control group and the healthy control group. But no difference of serum miR-499a-5p level was found in the benign control group and the healthy control group. Receiver operating characteristic (ROC) analysis showed that when used alone, the sensitivity and specificity of miR-499a-5p in the diagnosis of pancreatic cancer were better than that of CA199 (p < 0.05). Moreover, when serum miR-499a-5p was combined with CA199, the diagnostic value for pancreatic cancer increased significantly (p < 0.05). Dual luciferase reporter assay showed that PTEN was a target gene of miR-499a-5p. CONCLUSIONS: In summary, miR-499a-5p is a new, non-invasive biomarker, and the combination of miR-499a-5p and CA199 can improve the diagnostic sensitivity of pancreatic cancer.


Assuntos
Antígenos Glicosídicos Associados a Tumores/sangue , MicroRNA Circulante/sangue , MicroRNAs/sangue , Neoplasias Pancreáticas/diagnóstico , Idoso , Estudos de Casos e Controles , Linhagem Celular Tumoral , MicroRNA Circulante/genética , Feminino , Regulação Enzimológica da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Humanos , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , PTEN Fosfo-Hidrolase/genética , PTEN Fosfo-Hidrolase/metabolismo , Neoplasias Pancreáticas/sangue , Neoplasias Pancreáticas/genética , Valor Preditivo dos Testes , Prognóstico , Reprodutibilidade dos Testes
18.
Viruses ; 11(9)2019 09 18.
Artigo em Inglês | MEDLINE | ID: mdl-31540541

RESUMO

Porcine reproductive and respiratory syndrome virus (PRRSV) causes huge economic loss to the global swine industry. Even though several control strategies have been applied, PRRS is still not effectively controlled due to the continuous emergence of new variants and limited cross-protection by current vaccines. During the routine epidemiological investigation in 2017, two PRRSV variants were identified from a severe abortion farm and a clinically healthy farm, respectively. The viruses were isolated and denominated as XJ17-5 and JSTZ1712-12. Genomic sequencing indicated that their genomes are both 14,960 bp in length sharing 99.45% nucleotide identity. Sequence alignments identified a discontinuous 30-amino-acid deletion and a continuous 120-amino-acid deletion in nsp2 of both isolates. Genome-based phylogenetic analysis confirmed that XJ17-5 and JSTZ1712-12 belong to the HP-PRRSV subtype but form a new branch with other isolates containing the same 150-amino-acid deletion in nsp2. Pathogenic analysis showed that XJ17-5 is highly virulent causing 60% mortality, while JSTZ1712-12 is avirulent for piglets. Furthermore, fragment comparisons identified 34-amino-acid differences between XJ17-5 and JSTZ1712-12 that might be associated with the distinct virulence. The identification of highly homologous HP-PRRSV variants with new genetic feature and distinct virulence contributes to further analyze the pathogenesis and evolution of PRRSV in the field.


Assuntos
Variação Genética , Filogenia , Síndrome Respiratória e Reprodutiva Suína/virologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/genética , Vírus da Síndrome Respiratória e Reprodutiva Suína/patogenicidade , Animais , China , Surtos de Doenças/veterinária , Feminino , Genoma Viral , Alinhamento de Sequência , Deleção de Sequência , Homologia de Sequência , Suínos , Proteínas não Estruturais Virais/genética , Virulência
19.
Food Funct ; 10(10): 6312-6321, 2019 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-31517355

RESUMO

The present study aims to investigate the relationship between in silico experimental data and in vitro inhibitory data of polyphenols against α-glucosidase. The CDOCKER protocol in Discovery Studio was used to dock various polyphenols to the Saccharomyces cerevisiae α-glucosidase crystal structure. -CDOCKER energy values and the energy gap between the highest occupied molecular orbital energy and the lowest unoccupied molecular orbital energy were used to study its consistency with in vitro inhibitory data. The results showed that the correlation trend was trustworthy regardless of the data deviation and low correlation coefficient. Despite slight disagreements with some specific polyphenols, the docking data generally explained the effect of the groups (-OH, glycosyl, galloyl, and caffeoyl). The docking results showed that compound 7, a quercetin derivative, can be recommended as a lead antidiabetic compound, with additional anti-obesity effects. Galloyl and caffeoyl moieties are favorable to develop novel αG inhibitors.


Assuntos
Inibidores de Glicosídeo Hidrolases/química , Quercetina/química , Simulação por Computador , Simulação de Acoplamento Molecular , Estrutura Molecular , Polifenóis/química , Saccharomyces cerevisiae/enzimologia , Proteínas de Saccharomyces cerevisiae/química , alfa-Glucosidases/química
20.
Data Brief ; 25: 104314, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31453294

RESUMO

To compare porcine TLR8 (pTLR8) and human TLR8 (hTLR8) signaling pathways and downstream genes, we activated the pTLR8 and hTLR8 reporter cells with TLR8 agonist R848, and subjected the stimulated cells together with non-stimulated control cells for transcriptome analysis. There are 1157 differentially expression genes (DEGs) in R848 activated hTLR8 cells, whereas 502 DEGs in R848 activated pTLR8 cells. Among these DEGs, 804 genes are hTLR8 specific, 149 genes are pTLR8 specific, and 353 genes are hTLR8 and pTLR8 common. Related Results were published in reference [Ao, 2019].

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