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1.
Zhongguo Gu Shang ; 33(4): 348-52, 2020 Apr 25.
Artigo em Chinês | MEDLINE | ID: mdl-32351089

RESUMO

OBJECTIVE: To evaluate the clinical effect of arthroscopic 360° capsular release with concomitant rotator cuff repair for patients with rotator cuff tears in global stiff shoulder. METHODS: From December 2016 to December 2018, 247 patients full-thickness rotator cuff tear were treated with arthroscopic rotator cuff repair. Among them, 42 patients (17%) with global limitation of shoulder movement were treated with arthroscopic 360° capsular release operation at the same time of rotator cuff repair. The function of shoulder was evaluated by Constant-Murley score and visual analogue scales (VAS), flexion, abduction and external rotation on body side before and after operation. Sugaya classification was used to evaluatethe healing of rotator cuff. The number of shoulder dislocations during follow-up was recorded. RESULTS: Thirty nine of the 42 patients were followed up for 12 to 36 (22±6) months. The patients were 43 to 73 (57±7) years old at the time of operation. The Constant- Murley score increased from (43.6±6.3) before operation to (87.5±2.8) at final follow-up (P<0.001). VAS score decreased from (7.2±1.5) before operation to (1.0±0.9) at final follow-up (P<0.001). The flexion of shoulder increased from (46±14)° before operation to (148±11)° after operation (P<0.001), the abduction from (36±6)° before operation to (121±10)° after operation (P<0.001), and the external rotation on body side from (5±10)° before operation to (42±8)° after operation(P<0.001). One year after the operation, MRI showed thatⅠ-Ⅲ of Sugaya classification was clinical healing, 38 cases in total. CONCLUSION: Arthroscopic 360° capsular release with concomitant rotator cuff repair was used to treat rotator cuff tear in global stiff shoulder. Themain observation indexes were improved after operation, and the clinical effect was definite.

2.
Zhongguo Zhong Yao Za Zhi ; 45(5): 1159-1166, 2020 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-32237460

RESUMO

To evaluate the clinical efficacy and safety of Shentong Zhuyu Decoction in the treatment of lumbar disc herniation, in order to provide evidence for its clinical application. PubMed, Cochrane Library, EMbase, CNKI, WanFang, VIP, CBM and Clinical Trials.gov databases were sysmatically retrieved. Two researchers independently searched, screened and extracted data. Randomized controlled trials in line with the inclusion criteria were included in the study. The article quality was assessed with the bias risk tool in the Cochrane Handbook. Meta-analysis was performed using RevMan 5.3. A total of 604 articles were obtained from the retrieval, and 824 patients in 9 studies were finally selected through screening. The course of treatment was about 1 month, and all of the studies were completed in China. Meta-analysis showed that the efficacy of Shentong Zhuyu Decoction in the treatment of lumbar disc herniation was better than that of the control group(RR=1.19, 95%CI[1.12, 1.26], P<0.000 01), including the comparison with Yaotongning Capsules(RR=1.10, 95%CI[1.02, 1.19], P=0.01) and diclofenac sodium tablets(RR=1.26, 95%CI[1.11, 1.44], P=0.000 4). In VAS pain score, Shentong Zhuyu Decoction had also obvious advantages(MD=-3.70, 95%CI[-6.31,-1.09], P=0.005). In addition, the number of adverse events in the Shentong Zhuyu Decoction group was lower than that in the control group. Shentong Zhuyu Decoction can effectively alleviate discomfort symptoms of patients with LDH. It has an obvious clinical efficacy and less adverse reactions. However, restricted by the quality of the included literatures, prospective, multi-center, large-sample-size randomized controlled trials are still needed to further improve the clinical evidence of the effect of Shentong Zhuyu Decoction in the treatment of LDH.

3.
Zhongguo Zhong Yao Za Zhi ; 45(3): 609-616, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32237520

RESUMO

The aim of this paper was to investigate the key targets and mechanism of "Epimedii Folium-Paeoniae Radix Alba" in the treatment of lumbar disc herniation by means of network pharmacology. The currently recognized databases and analysis software at home and abroad were used to construct the network from drugs and diseases. The chemical components of Epimedii Folium and Paeo-niae Radix Alba were collected by using databases such as TCMSP, while their active components were determined and the action targets were predicted according to threshold screening and literature reports. The genes for lumbar disc herniation were collected by using GeneCards, OMIM, and DisGeNET databases. The drug targets were mapped to disease targets, and protein interaction network analysis for key targets, GO function enrichment analysis and KEGG signaling pathway enrichment analysis were performed. Finally, 23 active components of Epimedium Folium and 13 active components of Paeoniae Radix Alba were determined, and a total of 624 drug targets were obtained. After standardization, 214 drug targets were obtained. In addition, 306, 2 and 5 related targets of lumbar disc herniation were collected from GeneCards, OMIM, and DisGeNET database, respectively, and a total of 293 disease targets were obtained after deduplication. After the mapping of drug target and disease target, 44 common targets were obtained. PPI protein interaction network analysis showed that IL-6, TNF, AKT1, MAPK1, and VEGFA may be the core targets for the treatment of lumbar disc herniation. GO enrichment analysis identified 56 items(P<0.05), among which biological processes mainly included immune response, apoptosis, etc.; cell components mainly included extracellular space, extracellular region, etc.; molecular functions mainly included cytokine activity, metallopeptidase activity and so on. Through KEGG pathway enrichment analysis, 91 signaling pathways related to inflammation, metabolism, and senescence were identified, mainly including IL-17 signaling pathway and TNF signaling pathway and so on. "Epimedii Folium-Paeoniae Radix Alba" showed the characteristics of multi-channel and multi-target for the treatment of lumbar disc herniation. This study preliminarily explored the key targets for its role and the biological processes and signaling pathways involved. It was found that it may play a therapeutic role by affecting inflammation and immune regulation, which laid the foundation for further experimental verification.

4.
J Mol Model ; 26(2): 25, 2020 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-31927630

RESUMO

Based on the unique advantages of terahertz (THz) spectrum on the detection of energetic cocrystals, the low-temperature dependent THz spectra of CL-20/TNT cocrystal were investigated by using molecular dynamics (MD) simulations from 5 to 296 K, as well as three different crystal faces, (001), (120), and (010). When the temperature decreases below 95 K, we have observed two new peaks for CL-20/TNT cocrystal, at 4.58 and 5.99 THz, respectively. Also, the THz peaks below 1.5 THz gradually disappear under cooling from 296 to 5 K, and they should originate from the lattice thermal vibrations. THz absorption peaks of CL-20/TNT cocrystal reveal frequency shifting, linearly dependent on temperature. Four of them are red shift and other two are blue shift of THz vibrational peaks of CL-20/TNT cocrystal with the temperature increase. The frequency shifts can be attributed to the effects of lattice thermal expansion on inter-/intramolecular vibrational modes as well as their coupling. From the temperature-dependent THz spectra of different crystal faces, we further confirm the response of different kinds of intermolecular interactions on the THz spectrum of CL-20/TNT cocrystal. Graphical abstractThe intermolecular interactions and peak positions of THz spectra of CL-20/TNT cocrystal in the range of 0-6 THz versus temperature.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31995363

RESUMO

Dynamic tuning of terahertz (THz) wave has a great potential application as smart THz devices, such as switches, modulators, sensors, and so on. However, the realization of flexible THz modulation with high efficiency is rarely observed, which is nearly absent from the booming development and demands on flexible electronics. Here, we report a flexible THz modulation based on conductive polymer composites composed of thermoplastic polyurethane (TPU) and conductive particles (Ni). By designing the additive content of Ni particles, such a flexible layer exhibits resistivity change of 6-7 orders under tensile strain due to the formation of an electron-transport channel provided by the in situ evolution of the Ni network. It could be used to dynamically control the THz transmission with a giant modulation depth of around 96%, at a high strain operation (up to around 58.5%). Moreover, these characteristics are demonstrated to be available for highly tension sensitive THz spectroscopy and imaging. This work opens up a connection between flexible polymer-based composites and THz dynamic devices. It proposes an unprecedented flexible THz modulation with giant tuning efficiency and provides a scheme for contactless and passive tension sensors.

6.
BMC Surg ; 19(1): 161, 2019 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-31690303

RESUMO

BACKGROUND: Wound-related complications are an inevitable issue faced by spinal surgeons. Negative pressure drainage remains the most commonly used method to prevent postoperative hematoma and related complications. This prospective, randomized, controlled study was conducted to evaluate the efficacy of compression therapy following posterior lumbar interbody fusion, with emphasis on pain, anemia, and inflammation. METHODS: Sixty consecutive patients who have undergone posterior lumbar interbody fusion in the age range 43-78 years, with an average age of 59 years, were selected and randomly assigned into two groups. Factors, such as drainage volume, visual analog scale (VAS) pain score for back pain, white blood cell (WBC) count, red blood cell (RBC) count, hemoglobin (Hb) levels, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) levels assessed on the 1st, 3rd, and 10th days postoperatively, were compared between the two groups. RESULTS: The average follow-up was 6 months, ranging from 3 to 11 months. Drainage volume, VAS score, and CRP levels on the 10th day after the surgery were found to be significantly lower in the treatment group than in the control group. RBC count and Hb levels on the 3rd and 10th postoperative days were observed to be significantly higher in the treatment group than in the control group (P < 0.05). During discharge, the wounds of the patients of the both groups had healed and neither showed any symptoms of infection, hematoma, or necrosis. CONCLUSION: Compression therapy relieves pain, alleviates anemia, and the inflammatory response following posterior lumbar interbody fusion. TRIAL REGISTRATION: ChiCTR1800015825 on chictr.org.cn, April 23, 2018, the trial registry is Chinese Clinical Trial Registry.


Assuntos
Dor nas Costas/cirurgia , Vértebras Lombares/cirurgia , Fusão Vertebral/métodos , Adulto , Idoso , Feminino , Humanos , Inflamação/epidemiologia , Contagem de Leucócitos , Região Lombossacral , Masculino , Pessoa de Meia-Idade , Medição da Dor , Estudos Prospectivos , Resultado do Tratamento
7.
Zhongguo Gu Shang ; 32(9): 802-806, 2019 Sep 25.
Artigo em Chinês | MEDLINE | ID: mdl-31615174

RESUMO

OBJECTIVE: To explore the influence of individual characteristics of patients on the kinematics parameters of sitting lumbar spine manipulation. METHODS: From January 2016 to December 2016, 30 patients with degenerative lumbar spondylolisthesis were treatede, including 18 males and 12 females, aged from 45 to 61(52.33±2.34) years old, with a course of 2 to 72(29.13±3.23) months. Motion capture technique was used to measure the kinematics parameters of the same manipulator in sitting lumbar spine manipulation for 30 patients, including rotation time, maximum speed and maximum acceleration. The parameters of the left and right hands of the operator were different. The effects of individual characteristics on the kinematics parameters of sitting lumbar spine manipulation were analyzed. RESULTS: The kinematic and mechanical parameters of manipulation were as follows:spin time, maximum speed, maximum acceleration, the manipulation done by right or left hand showed the similar parameters(P>0.05). According to multivariate linear regression, there was a significant correlation between the age of the patient and the rotation time of the operator(P<0.01); the height of patient had a significant correlation with the maximum speed and maximum acceleration of the operator(P<0.01). CONCLUSIONS: There is no difference between the manipulation of left and right hand in clinical application. Age and height are important influencing factors of sitting lumbar rotation manipulation.


Assuntos
Manipulação da Coluna , Postura Sentada , Fenômenos Biomecânicos , Feminino , Humanos , Vértebras Lombares , Masculino , Pessoa de Meia-Idade , Amplitude de Movimento Articular
8.
Opt Lett ; 44(7): 1630-1633, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30933108

RESUMO

We report a high-performance terahertz (THz) modulator with dual operation mode. For the pulse operation mode, the proposed THz modulator has the advantage of high modulation depth (MD) and can operate in a broadband frequency range. We have experimentally achieved a MD larger than 90% for the fifth-order pulse THz echo at 0.8 THz, and the MD stays larger than 75% in a broadband frequency range larger than 1 THz, whereas, for the coherent operation mode, the Fabry-Perot (F-P) interference effect has been taken into consideration and a MD larger than 75% at 0.76 THz has also been realized.

9.
J Biomed Nanotechnol ; 15(5): 910-920, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30890223

RESUMO

The application of terahertz imaging in biomedical diagnostics is limited by the poor imaging contrast due to the strong absorption of terahertz waves by water molecules in the living body. To resolve this problem, gold nanorods (GNRs) have been considered as a possible contrast agent. However, the conventional preparation of GNRs requires using cetyltrimethylammonium bromide (CTAB) as a stabilizing surfactant, which is toxic to animals at high exposures. In this work, a modified seed-mediated growth method was proposed to synthesize silica-coated GNRs, which exhibited better stability and biocompatibility and the same cellular uptake efficiency as compared to those of uncoated GNRs. Moreover, silica-coated GNRs showed higher photothermal efficiency caused by the localized surface plasmon resonance that increased the terahertz reflection amplitude. In particular, the in vitro terahertz imaging of prostate cancer cells demonstrated noticeably higher contrast when silica-coated GNRs were used as a contrast agent. Hence, this study suggests a stable, biocompatible, and efficient contrast-enhancing agent for terahertz imaging, which can expand the scope of its clinical applications.


Assuntos
Nanotubos , Imagem Terahertz , Animais , Compostos de Cetrimônio , Ouro , Masculino , Neoplasias da Próstata , Dióxido de Silício
10.
Opt Lett ; 44(1): 21-24, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30645535

RESUMO

We demonstrate terahertz (THz) wave near-field imaging with a spatial resolution of ∼4.5 µm using single-pixel compressive sensing enabled by femtosecond-laser (fs-laser) driven vanadium dioxide (VO2)-based spatial light modulator. By fs-laser patterning a 180 nm thick VO2 nanofilm with a digital micromirror device, we spatially encode the near-field THz evanescent waves. With single-pixel Hadamard detection of the evanescent waves, we reconstructed the THz wave near-field image of an object from a serial of encoded sequential measurements, yielding improved signal-to-noise ratio by one order of magnitude over a raster-scanning technique. Further, we demonstrate that the acquisition time was compressed by a factor of over four with 90% fidelity using a total variation minimization algorithm. The proposed THz wave near-field imaging technique inspires new and challenging applications such as cellular imaging.

11.
Rev Sci Instrum ; 90(12): 123105, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31893805

RESUMO

A quick simultaneous multispot laser induced breakdown spectroscopy (LIBS) system has been proposed. The basic idea is to combine the Diffraction Beam Splitter (DBS) with the linear-to-linear fiber bundle. The DBS divided the incident laser beam into five sub-beams, and then, a lens focused the incident sub-beam to produce a plasma array, where the distance between the neighbor subplasma was constant and the plasma emission was imaged on the fiber bundle. Each ablated spot on the sample generated the corresponding spectroscopy signals, which were collected by the defined fibers of the fiber bundle, propagated to the spectrograph slit, and then analyzed by the intensified charge-coupled device (ICCD) detector, where the two-dimensional capability of the charge-coupled device detectors was explored for the spectroscopy and position analyses. The five spectroscopy ribbon presented on the ICCD corresponded to the plasma radiation of the five ablated spots. The feasibility of a simultaneous multipoint spectroscopy detection at a single pulse ablation was confirmed by subjecting to the spectroscopy analyses of a copper plane, layered material, and magnesium-molybdenum mixture. The multispot LIBS system can be used in the ejecta research of detonation physics, fluid physics, and so on.

12.
Opt Express ; 26(21): 28051-28066, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-30469861

RESUMO

Ultrafast detection and switching of light are key processes in high-speed optoelectronic devices. However, the performances of VO2-based optoelectronics are strongly degraded by photothermal. The mechanism of the latter is still unclear. Here, by using femtosecond-laser (fs-laser) driven kinetic terahertz wave absorption, we quantitatively separate slow photothermal response and ultrafast photodoping response (e.g. light-induced insulator-to-metal transition) from second- to picosecond-timescales, and discover the competing interplay between them. With self-photothermal (mainly determined by fs-laser pulse repetition rate and pump fluence), the ultrafast transition time was degraded by 190% from 50 ps to 95 ps, the ultrafast transition threshold was decreased to 82% from 11mJ/cm2 to 9mJ/cm2, while the amplitudes of the two photoresponse are competing. Percolation theory, along with the macroscopic conductivity response, is used to explain the competing interplay. Our findings are relevant for designing and optimizing VO2-based ultrafast optoelectronic devices.

13.
Opt Express ; 26(23): 30655-30666, 2018 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-30469959

RESUMO

We proposed an ultrasensitive specific terahertz sensor consisting of two sets of graphene micro-ribbon with different widths. The interference between the plasmon resonances of the wide and narrow graphene micro-ribbons gives rise to the plasmon induced transparency (PIT) effect and enables ultrasensitive sensing in terahertz region. The performances of the PIT sensor have been analyzed in detail considering the thickness and refractive index sensing applications using full wave electromagnetic simulations. Taking advantage of the electrical tunability of graphene's Fermi level, we demonstrated the specific sensing of benzoic acid with detection limit smaller than 6.35 µg/cm2. The combination of specific identification and enhanced sensitivity of the PIT sensor opens exciting prospects for bio/chemical molecules sensing in the terahertz region.

14.
Zhongguo Gu Shang ; 31(7): 627-634, 2018 Jul 25.
Artigo em Chinês | MEDLINE | ID: mdl-30103586

RESUMO

OBJECTIVE: To compare effect of serum of Bushen Huoxue prescription on rabbit with intervertebral disc motion segments in vitro culture. METHODS: Twenty-four New Zealand white rabbits aged from 4 to 6 months and weighted from 2.5 to 3 kg were divided into medicated group and control group, 7 in each group. Rabbits were excuted under condition of asepsis, 28 spinal motion segments were taken out in each group, and segments were loaded in spinal motion segments in vitro and cultured in culture apparatus. Nutrient solution of medicated group contain 10% serum of Bushen Huoxue prescription, and 10% blank serum in control group. Seven discs between two groups were taken out and observed by histomorphology, proteoglycan PAS/AB stining, collagen II immunohistochemical staining, AGG, Col2aI by PCR test before culture and on the 3th, 7th and 14th day after culture. RESULTS: Histomorphology results showed the formation of medicated group was better than that of control group at 1 week after culture. PAB/AB test results showed content of poteoglycan between two groups were decreased. Imunohistochemical results showed content of collagen II in medicated group were obviously increased than that of control group at 3 days, but decreased obviously at 1 week than that of control group. PCR results showed expression of Agg in medicated group was obviously decreased than that of control group, but no statistical significance between two groups on the 7 th and 14 th day. Expression of Col2aI between two groups at 3 days were increased, and medicated group increased obviously more, while there were no significant difference between two groups. CONCLUSIONS: Serum of Bushen Huoxue prescription could delay intervertebral disc degeneration at short time for it is relate with improving circulation, inhibiting inflammatory, regulating extracellular matrix, so the prescription plays an important role in early prevention and treatment for intervertebral disc degeneration.


Assuntos
Degeneração do Disco Intervertebral , Disco Intervertebral , Animais , Medicamentos de Ervas Chinesas , Matriz Extracelular , Coelhos
15.
Opt Express ; 26(11): 14132-14142, 2018 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-29877456

RESUMO

Terahertz wave imaging offers promising properties for non-destructive testing applications in the areas of homeland security, medicine, and industrial inspection. However, conventional optical lenses are heavy and bulky and difficult to integrate. An all-dielectric metasurface provides an attractive way to realize a planar lens of light weight that is ultrathin and offers ease of integration. Terahertz lenses based on various metasurfaces have been studied, especially for the application of wave focusing, while there are few experimental demonstrations of terahertz wave imaging lenses based on an all-dielectric metasurface. In the present work, we propose a metalens based on an all-dielectric metasurface with a sub-wavelength unit size of 0.39λ for terahertz wave imaging and experimentally demonstrate its performance in focusing and imaging. A large numerical aperture metalens was fabricated with a focal length of 300λ, radius of 300λ, and numerical aperture of 0.707. The experimental results show that the lens can focus THz waves with an incident angle up to 48°. More importantly, clear terahertz wave images of different objects were obtained for both different cases of forward- and inverse-incident directions, which demonstrate the reversibility of the metalens for imaging. Such a metalens provides a way for realization of all-planar-lens THz imaging system, and might find application in terahertz wave imaging, information processing, microscopy, and others.

16.
Biomed Opt Express ; 9(1): 14-24, 2018 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-29359084

RESUMO

We demonstrated that attenuated total reflectance terahertz time-domain spectroscopy (ATR THz-TDS) is able to monitor oxidative stress response of living human cells, which is proven in this work that it is an efficient non-invasive, label-free, real-time and in situ monitoring of cell death. Furthermore, the dielectric constant and dielectric loss of cultured living human breast epithelial cells, and along with their evolution under oxidative stress response induced by high concentration of H2O2, were quantitatively determined in the work. Our observation and results were finally confirmed using standard fluorescence-labeled flow cytometry measurements and visible fluorescence imaging.

17.
Opt Express ; 25(15): 17066-17075, 2017 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-28789203

RESUMO

A new approach for dual-mode (namely broadband mode and narrowband mode) terahertz (THz) pulses generation in a single lithium niobate (LN) crystal excited by spatially shaped tilted-pulse-front femtosecond (fs) laser pulse was proposed and experimentally demonstrated. The two THz emission modes are generated simultaneously while spatially separated. Both central frequency and bandwidth of narrowband THz emission is controllable by in situ tuning the spatial modulation period and beam size of the fs-laser, and the broadband (0.1-1.5 THz) THz emission keeps almost unchanged while tuning the narrowband emission. Further optimization achieves the narrowband THz emission with energy spectral density up to 0.27 µJ/THz and with bandwidth narrowly down to 23 GHz. Such dual-mode THz source is useful for nonlinear THz optics, such as selected resonant THz excitation with broadband THz probe spectroscopy of crystalline matters.

18.
Sci Rep ; 7(1): 5176, 2017 07 12.
Artigo em Inglês | MEDLINE | ID: mdl-28701795

RESUMO

While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin deficit in mouse and rhesus monkey brains. The THz refractive index and absorption coefficient of paraffin-embedded brain tissues from both normal and mutant dysmyelinating mice are shown. Principal component analysis of time-domain THz signal (PCA-tdTHz) and absorption-refractive index relation of THz spectrum identified myelin deficit without exogenous labeling or any pretreatment. Further, with the established PCA-tdTHz, we evaluated the severity of myelin deficit lesions in rhesus monkey brain induced by experimental autoimmune encephalomyelitis, which is the most-studied animal model of multiple sclerosis. The results well matched the pathological analysis, indicating that PCA-tdTHz is a quick, powerful, evolving tool for identification and evaluation myelin deficit in preclinical animals and potentially in para-clinical human biopsy.


Assuntos
Encéfalo/metabolismo , Encéfalo/patologia , Doenças Desmielinizantes/genética , Doenças Desmielinizantes/metabolismo , Proteínas da Mielina/deficiência , Espectroscopia Terahertz , Animais , Biomarcadores , Doenças Desmielinizantes/patologia , Modelos Animais de Doenças , Encefalomielite Autoimune Experimental/etiologia , Encefalomielite Autoimune Experimental/metabolismo , Encefalomielite Autoimune Experimental/patologia , Macaca mulatta , Camundongos , Camundongos Knockout , Espectroscopia Terahertz/métodos
19.
Zhongguo Gu Shang ; 30(10): 926-932, 2017 Oct 25.
Artigo em Chinês | MEDLINE | ID: mdl-29457415

RESUMO

OBJECTIVE: To study the effect of Bushen Huoxue decoction on calcification of cartilage endplate in lumbar vertebrae. METHODS: Six healthy male gerbils with 2-month-old were selected as normal control group, and 24 7-month-old healthy male gerbils were fed to 12-month-old to establish the aged gerbil model. Thirty gerbils were randomly divided into five groups as follow: the normal control group (n=6), model group (n=6, normal saline 4 ml/kg, intragastric 30 d), Bushen Huoxue low dose group(n=6, 1.9× 10⁻ ³ ml/g given Bushen Huoxue recipe orally, 30 d), Bushen Huoxue middle dose group(n=6, 3.8× 10⁻ ³ ml/g given Bushen Huoxue recipe orally, 30 d), Bushen Huoxue high dose group(n=6, 7.6× 10⁻ ³ ml/g given Bushen Huoxue recipe orally, 30 d), the intervention group administered for 1.36 g from 7-month-old age, 30 d. The animals were sacrificed at the age of 2 months in the normal control group and 12 months of age in the other groups. The morphology of the lumbar vertebral cartilage endplate, the area of vascular bud, the ratio of non-calcified/calcified layer were analysis by HE chromosome visual method. The expression of type X collagen and BMPs in cartilage endplates were detected by rabbit monoclonal immunohistochemical staining. RESULTS: The relative area of the vascular buds cartilage endplate measurements showed that compared with the model group, middle dose group and normal control group increased (P<0.05), high and low dose groups all had different degrees of increase, but no statistical significance(P>0.05). The ratio of cartilage endplate thickness of non-calcified/calcified showed that compared with the model group, Bushen Huoxue middle dose, normal control group increased, with statistical significance(P<0.05), and high and low dose groups all had different degrees of increase, but there were no statistical significance(P>0.05). Compared with the model group, the expression of type X collagen in the cartilage endplate of the normal group, the Bushen Huoxue low, middle and high dose groups decreased, and had statistical significance(P<0.01); compared with the model group, the expression of BMPs in the normal group, Bushen Huoxue middle dose group increased, with statistically significant(P<0.01), while the high and low dose groups increased in different degrees, but there was no statistical significance(P>0.05). CONCLUSIONS: Bushen Huoxue prescription can delay the calcification of cartilage endplate in the process of aging, suggesting that it can be used as a preventive medicine for early disc degeneration.


Assuntos
Calcinose/tratamento farmacológico , Doenças das Cartilagens/tratamento farmacológico , Medicamentos de Ervas Chinesas/uso terapêutico , Vértebras Lombares , Envelhecimento , Animais , Gerbillinae , Degeneração do Disco Intervertebral/prevenção & controle , Masculino , Distribuição Aleatória
20.
Zhongguo Gu Shang ; 30(3): 241-246, 2017 Mar 25.
Artigo em Chinês | MEDLINE | ID: mdl-29349963

RESUMO

OBJECTIVE: To evaluate the stability of teaching robot of rotation-traction manipulation. METHODS: Operators were required to get the hang of rotation-traction manipulation and had clinical experience for over 5 years. The examination and data processing of the ten operators in our research were collected by the teaching robot of rotation-traction manipulation. Traction, pulling force, maximum force, pulling time, rotational amplitude and pitch range were recorded and compared for five times(G1, G2, G3, G4 and G5). The qualification rates were analyzed to evaluate the stability of teaching robot of rotation-traction manipulation. RESULTS: Nonconforming items were found in G1 and G2, for instance, pulling force(P=0.074), maximum force(P=0.264) and rotational amplitude (P=0.531). There was no statistically difference. None nonconforming item was found in G3, G4 and G5. All data were processed by SPSS and One-way ANOVA was used to analysis. Pulling force was found statistically different in G1, compared with G4 and G5(P=0.015, P=0.006). Maximum force was found statistically different in G1, compared with G4 and G5 (P=0.021, P=0.012). None differences were found in other comparisons (P>0.05). CONCLUSIONS: The teaching robot of rotation-traction manipulation used in our research could provide objective and quantitative indices and was considered to be an effective tool of assessing the rotation-traction manipulation.


Assuntos
Manipulações Musculoesqueléticas/métodos , Robótica , Rotação , Tração , Manipulações Musculoesqueléticas/instrumentação , Pesquisa
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