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1.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 41(4): 575-578, 2019 Aug 30.
Artigo em Chinês | MEDLINE | ID: mdl-31484625

RESUMO

Autoimmune pancreatitis(AIP)is radiologically characterized by sausage-like diffuse swelling of the pancreatic parenchyma but may also be found as a localized mass that is easily misdiagnosed as a pancreatic neoplasm.AIP presenting as multifocal masses is rare.Here we report a case of multifocal IgG4-related AIP,in which the lesions grew in size and finally fused to become radiologically typical.

2.
Water Res ; 164: 114925, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31382155

RESUMO

Increasing studies indicate that magnetite addition could accelerate the methanogenesis via enhancing direct interspecies electron transfer (DIET)-based anaerobic syntrophy. However, magnetite is found to run off in continuous bioreactor, and the effect of magnetite loss on syntrophic aggregates is still underreported. In this study, two EGSB reactors (RM with magnetite-enhanced sludge, and RB as a control) were operated to investigate the magnetite behavior in continuous bioreactor and the corresponding response of syntrophic aggregates. Results showed that magnetite in RM was washed out gradually in form of iron ions, and a slightly acidic niche was supposed to be the major cause. Nevertheless, candidate DIET partners like Geobacter and Methanothrix along with syntrophic volatile fatty acids (VFAs)-degrading microbes were enriched in RM. In addition, the improved redox activity of extracellular polymeric substance (EPS), higher sludge conductivity and electron transport activity suggested that the DIET ability of sludge in RM was still enhanced, which favors the syntrophic metabolism of VFAs. Interestingly, syntrophic partners were loosely combined under the condition of high organic loading rate (OLR) in the presence of magnetite, but with gradual loss of magnetite, dense and active anaerobic granular sludge (AGS) was formed in RM. This study provided a comprehensive understanding of magnetite behavior in continuous bioreactor and the response of syntrophic aggregates. The robust DIET-based syntrophy after magnetite adding could favor the high-efficient anaerobic wastewater treatment and resource recovery in the future, and further investigations on magnetite resupply and the mechanism of magnetite enriching candidate DIET partners are recommended.

3.
Medicine (Baltimore) ; 98(33): e16727, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31415366

RESUMO

Our study was designed to construct nomograms to predict the overall survival (OS) and cancer-specific survival (CSS) of lip carcinoma patients.A search of the Surveillance, Epidemiology, and End Results (SEER) database provided us with detailed clinical data of the 1780 lip carcinoma patients. On the basis of the credible random split-sample method, the 1780 patients were placed into 2 groups, with 890 patients in the modeling group and 890 patients in the counterpart's group (proportion = 1:1). By employing Kaplan-Meier univariate and Cox multivariate survival analyses based on the modeling cohort, the nomograms were developed and then used to divide the modeling cohort into low-risk cohort and high-risk cohort. The survival rates of the 2 groups were calculated. Internal and external evaluation of nomogram accuracy was performed by the concordance index (C-index) and calibration curves.With regard to 5- and 8-year OS and CSS, the C-indexes of internal validation were 0.762 and 0.787, whereas those of external validation reached 0.772 and 0.818, respectively. All the C-indexes were higher than 0.7. The survival curves of the low-risk cohort were obviously better than those of the high-risk cohort.Credible nomograms have been established based on the SEER large-sample population research. We believe these nomograms can contribute to the design of treatment plans and evaluations of individual prognosis.


Assuntos
Carcinoma/mortalidade , Neoplasias Labiais/mortalidade , Nomogramas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Prognóstico , Modelos de Riscos Proporcionais , Estudos Retrospectivos , Programa de SEER , Taxa de Sobrevida , Adulto Jovem
4.
Artigo em Inglês | MEDLINE | ID: mdl-31414500

RESUMO

RATIONALE: Floral volatiles are commonly present only at trace amounts and can be degraded or lost during vapor collection, which is often challenging from the analytical standpoint. Osmanthus fragrans Lour. is a widely cultivated plant known for the highly distinct fragrance of its flowers. The identification of specific volatile organic compounds (VOCs) and molecular differentiation of O. fragrans without any chemical pretreatment and VOC collection are important. METHODS: Twenty-eight VOCs released by the flowers from ten different cultivars of O. fragrans were identified using neutral desorption extractive atmospheric pressure chemical ionization mass spectrometry (ND-EAPCI-MS) without any chemical pretreatment or VOC collection. Chemical identification was performed by high-resolution MSn analysis and whenever possible was confirmed by the analysis of standards. RESULTS: According to our literature search, nine of the identified VOCs, 3-buten-2-one, cyclohexadiene, 2-methylfuran, 3-allylcyclohexene, cuminyl alcohol, hotrienol oxide, epoxy-linalool oxide, N-(2-hyrdoxyethyl) octanamide, and 3-hydroxy-dihydro-ß-ionone, have not been reported in O. fragrans in earlier studies. Confident differentiation between ten different cultivars of O. fragrans was achieved by the principal component analysis of the mass spectrometric results. CONCLUSIONS: The results of our ND-EAPCI-MS analysis substantially increase our knowledge about the chemistry of the O. fragrans floral fragrance and demonstrate the power of this technique for direct molecular profiling for plant recognition or in biotechnological applications.

5.
Ying Yong Sheng Tai Xue Bao ; 30(7): 2218-2230, 2019 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-31418224

RESUMO

Quercus mongolica and Phellodendron amurense are two important broad-leaved species in temperate forests of Northeast China. It is critical to explore their responses to climate change for supporting management, protection, and restoration of the broad-leaved forest in Northeast China under the future climate change scenario. Three sampling sites along a longitude gradient, Heilun, Tieli and Yichun, were set up in the Xiaoxing'an Mountains. Dendrochronological methods were used to establish standard chronologies for Q. mongolica and P. amurense. Correlation analyses were conducted between these chronologies and local climatic factors to establish the spatial and temporal variations in growth-climate relationship of Q. mongolica and P. amurense. The results showed that the radial growth of P. amurense was sensitive to temperature, while that of Q. mongolica was limi-ted by both temperature and precipitation. The temperature sensitivities of these two species were different. High spring temperature inhibited the radial growth of Q. mongolica, but promoted that of P. amurense. The limiting effect of high maximum temperature in summer on radial growth of Q. mongolica was significantly higher than that of P. amurense. With the increases of longitude (water availability), the correlation coefficients between radial growth of Q. mongolica and precipitation gradually weakened, while P. amurense didn't change. The physiological characteristics of those tree species was the key factors affecting their growth-climate relationship. With the significant warming since the 1976, the growth trend of P. amurense increased, whilst that of Q. mongolica decreased. Deteriorated drought stress caused by warming and difference in the species' ability to cope with water deficits might be the main reasons for different responses of two species, and for the divergence phenomenon occurring for Q. mongolica. If warming continues or worsens in the future, the growth of Q. mongolica may decline due to the intensified drought stress, while that of P. amurense may be less affected or be slightly enhanced.


Assuntos
Mudança Climática , Phellodendron/crescimento & desenvolvimento , Quercus/crescimento & desenvolvimento , China , Florestas , Árvores
6.
J Magn Reson Imaging ; 2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31313426

RESUMO

CONTRACT GRANT SPONSOR: Chinese Academy of Medical Sciences (CAMS) Initiative for Innovative Medicine; Contract grant number: 2017-I2M-1-001; Contract grant sponsor: Outstanding Youth Fund of Peking Union Medical College Hospital; Contract grant number: JQ201704; Contract grant sponsor: National Natural Science Foundation of China; Contract grant number: 81871512; Contract grant sponsor: National Public Welfare Basic Scientific Research Program of Chinese Academy of Medical Sciences; Contract grant numbers: 2018PT32003 and 2017PT32004. BACKGROUND: Both compressed-sensing (CS) and gradient- and spin-echo (GRASE) sequences can achieve 3D magnetic resonance cholangiopancreatography (MRCP) with a single breath-hold (BH). This work hypothesized that compared with conventional navigator-triggered (NT)-MRCP, the two BH-MRCP protocols, GRASE and CS, may provide better imaging quality, especially for patients with irregular breathing. PURPOSE: To evaluate and compare the image quality and diagnostic performance of three MRCP protocols. STUDY TYPE: Prospective. SUBJECTS: Seventy-four patients suspected to have duct-related pathologies were enrolled. FIELD STRENGTH: 3.0T. SEQUENCES: NT-MRCP, BH-CS-MRCP, and BH-GRASE-MRCP. ASSESSMENT: Breath regularity was evaluated subjectively according to the respiratory waves. The acquisition time was compared. The pancreaticobiliary system was divided into 12 segments and evaluated on a 5-point scale. The diagnostic performance of the three MRCPs was evaluated and compared. STATISTICAL TESTS: The Friedman test with a post-hoc test, receiver operating characteristic (ROC) curve analysis, McNemar test, and Kendall's W test were used. RESULTS: The BH-MRCP decreased the scan time significantly (P < 0.05). The overall imaging scores of GRASE-MRCP and CS-MRCP were significantly higher than that of NT-MRCP for patients with irregular breathing (4.283 and 4.283 vs. 3.000, both P < 0.05). Compared with NT-MRCP, the diagnostic performance of BH-CS and BH-GRASE MRCP was significantly improved for patients with irregular breathing (AUC = 0.860 and 0.863 vs. 0.572, both P < 0.001). DATA CONCLUSION: Compared with conventional NT-MRCP, the overall imaging quality and diagnostic performance of BH-CS and BH-GRASE MRCP were not significantly different for patients with regular breathing and significantly superior for patients with irregular breathing. LEVEL OF EVIDENCE: 2 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2019.

7.
Artigo em Inglês | MEDLINE | ID: mdl-31329554

RESUMO

Instance segmentation is a challenging computer vision problem which lies at the intersection of object detection and semantic segmentation. Motivated by plant image analysis in the context of plant phenotyping, a recently emerging application field of computer vision, this paper presents the Exemplar-Based Recursive Instance Segmentation (ERIS) framework. A three-layer probabilistic model is firstly introduced to jointly represent hypotheses, voting elements, instance labels and their connections. Afterwards, a recursive optimization algorithm is developed to infer the maximum a posteriori (MAP) solution, which handles one instance at a time by alternating among the three steps of detection, segmentation and update. The proposed ERIS framework departs from previous works mainly in two respects. First, it is exemplar-based and model-free, which can achieve instance-level segmentation of a specific object class given only a handful of (typically less than 10) annotated exemplars. Such a merit enables its use in case that no massive manually-labeled data is available for training strong classification models, as required by most existing methods. Second, instead of attempting to infer the solution in a single shot, which suffers from extremely high computational complexity, our recursive optimization strategy allows for reasonably efficient MAP-inference in full hypothesis space. The ERIS framework is substantialized for the specific application of plant leaf segmentation in this work. Experiments are conducted on public benchmarks to demonstrate the superiority of our method in both effectiveness and efficiency in comparison with the state-of-the-art.

8.
J Diabetes Res ; 2019: 5237371, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31281851

RESUMO

Background: To examine the association between morbid events and metabolic syndrome (MS) in patients with type 2 diabetes mellitus (T2DM). Methods: A prospective, longitudinal, multicenter study was conducted at 13 community health centers associated with Beijing Tongren Hospital. From 2008 to 2015, there have been 3,525 T2DM patients being managed based on the Chinese guideline for T2DM. The morbid events included macrovascular events, diabetic kidney disease, ophthalmologic events, cancer, and all-cause death. Results: At baseline, there were 2,708 people with MS and 817 without MS. After a seven-year management, there were 351 (12.96%) events in MS people and 74 (9.06%) events in people without MS (p = 0.003). The prevalence of macrovascular events (6.06%) was much higher in MS people than in people without MS (3.79%, p = 0.013). Cox regression analysis showed an association between MS and morbid events even after adjusting for confounding variables (adjusted hazard ratio = 1.44). MS was also associated with macrovascular events (adjusted hazard ratio = 1.96). The occurrence of morbid events and macrovascular events was increased when the numbers of metabolic abnormalities were 1, 2, 3, and 4 (p < 0.001). There was no continuously statistically significant difference in the cumulative prevalence of morbid events between patients with MS and patients without MS during the first five years. However, after six or seven years, the cumulative prevalence of morbid events in patients with MS was continuously significantly higher than that in patients without MS (11.00% vs. 8.20%, 12.96% vs. 9.06%, p < 0.05). Conclusions: T2DM with MS had higher incidence of morbid events, especially cardiovascular events, even after integrated management. The occurrence of morbid and macrovascular events increased as the number of metabolic abnormalities increased. MS was associated with increased risk of morbid events by 44% and macrovascular events by 96%. It would take at least six years to observe the association between MS and morbid events in T2DM.

9.
J Mol Cell Biol ; 2019 Jul 23.
Artigo em Inglês | MEDLINE | ID: mdl-31336385

RESUMO

ZC4H2 encodes a C4H2 type zinc-finger nuclear factor, the mutation of which has been associated with disorders with various clinical phenotypes in human, including developmental delay, intellectual disability and dystonia. ZC4H2 has been suggested to regulate spinal cord patterning in zebrafish as a co-factor for RNF220, an ubiquitin E3 ligase involved in Gli signaling. Here we showed that ZC4H2 and RNF220 knockout animals phenocopies each other in spinal patterning in both mouse and zebrafish, with mispatterned progenitor and neuronal domains in the ventral spinal cord. We showed evidence that ZC4H2 is required for the stability of RNF220 and also proper Gli ubiquitination and signaling in vivo. Our data provides new insights into the possible etiology of the neurodevelopmental impairments observed in ZC4H2 associated syndromes.

10.
Pest Manag Sci ; 2019 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-31270930

RESUMO

BACKGROUND: Dengue fever is caused by the spread of dengue virus by Aedes mosquito vectors. Currently, the most effective way to control dengue is by preventing mosquitoes from spreading the disease. Arisaema fargesii is a Chinese herbal medicine commonly used to repel mosquitoes. In our laboratory, anti-mosquito chemical components were extracted from A. fargesii, and the effects of these substances on mosquito larvae were examined. RESULTS: In total, 48 compounds corresponding to 98.79% of the total oil were identified and the major compounds identified were linalool (12.38%), carvacrol (8.27%), eugenol (5.21%), and ß-selinene (5.36%). Essential oil had larvicidal activity against Ae. aegypti and Ae. albopictus with LC50 values of 40.49 mg/L, 47.01 mg/L, respectively. The LC50 values of carvacrol, eugenol, linalool and ß-selinene were 32.78, 56.34, 70.56, 136.03 mg/L against Ae. aegypti larvae, and 39.08, 52.07, 82.34, 151.74 mg/L, respectively, against Ae. albopictus larvae. Biochemical assays of Aedes larvae showed that the activities of acetylcholinesterase (AChE), monooxygenases (MO), glutathione-S-transferase (GST), p-Nitrophenyl acetate (p-NPA) esterase, α-esterase and ß-esterase were significantly affected by carvacrol. Essential oil induced the detoxification mechanism for the action of GST and MO. CONCLUSION: The result indicates that essential oil of A. fargesii and its isolated constituent have good inhibitory effects on the defense enzymes of Aedes mosquito larvae. A. fargesii essential oil can be used to control Aedes mosquito larvae to prevent the spread of dengue fever. © 2019 Society of Chemical Industry.

11.
BMC Musculoskelet Disord ; 20(1): 340, 2019 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-31351465

RESUMO

BACKGROUND: To compare the efficacy and safety of simultaneous vs staged unicompartmental knee arthroplasty (UKA) for bilateral unicompartmental osteoarthritis of the knee. METHODS: We performed a retrospective analysis of prospectively collected data on 93 patients with bilateral knee medial compartment arthritis underwent simultaneous UKA (Group A, 39) or staged UKA (Group B, 54 cases) from January 2008 to December 2015. Group A: 6 males and 33 females aged 64.9 ± 7.7 years; Group B: 5 males and 49 females aged 64.2 ± 6.4 years. There were no statistically significant differences in pre-operative age, sex ratio, or body weight index between the groups (P > 0.05). Groups were compared in terms of total anesthesia time, volume of drainage, blood transfusion rate, hemoglobin level on post-operative day 3, total post-operative inpatient days, treatment expenses, post-operative therapeutic effect (KSS scores), and complications. RESULTS: All patients had follow-up visits post-operatively. The follow-up visit interval was 32-133 months and 41.9 months on average. Total anesthesia time, postoperative length of hospital stay, and hospitalization expenses in Group A were significantly less than those of Group B (P < 0.05). Hemoglobin levels in Group A were significantly lower than those of Group B at post-op day 3 (P < 0.05). However, no significant differences in volume of drainage, the rate of transfusion, complications, and KSS scores were detected between Groups A and B (P > 0.05). CONCLUSIONS: Both simultaneous and staged UKA achieved the desired therapeutic effect in treatment of bilateral knee medial compartment arthritis. However, simultaneous UKA reduced the cost and the postoperative length of hospital stay without increasing post-operative complications.

12.
Bioresour Technol ; 289: 121713, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31276993

RESUMO

Alkaline fermentation technology is an effective method for resource recovery, e.g., short-chain fatty acids (SCFAs), but the acidification process needs to be further enhanced. To improve the release and acidification of organic matters in waste activated sludge (WAS) fermentation simultaneously, a novel method was proposed with magnetite addition under alkaline condition in this study. Compared with the control, SCFAs and acetic acids yields increased by 21.2% ±â€¯3.1% and 31.0% ±â€¯1.2% in the 0.6 g/gVSS magnetite-based system, respectively. Besides, the activities of α-glucosidase, protease and acetate kinase (AK) were enhanced with magnetite addition, and the abundance of acidogenic microbes was improved obviously. Furthermore, magnetite reduced the release of PO43--P significantly via the precipitation reaction. Of all, a novel side-stream WAS hydrolysis and acidification process based on magnetite addition under suitable alkaline condition is proposed, realizing the efficient recovery of carbon and phosphorus resources along with wastewater and WAS treatment.


Assuntos
Ácidos Graxos Voláteis/biossíntese , Óxido Ferroso-Férrico/farmacologia , Esgotos , Álcalis , Fermentação/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Hidrólise
13.
Environ Sci Technol ; 53(15): 9269-9278, 2019 Aug 06.
Artigo em Inglês | MEDLINE | ID: mdl-31288521

RESUMO

Coal combustion in low-efficiency household stoves results in the emission of large amounts of nonmethane organic compounds (NMOCs), including intermediate-volatility compounds (IVOCs) and semivolatile organic compounds (SVOCs). This conceptual picture is reasonably well established, however, quantitative assessment of I/SVOC emissions from household stoves is rare. We used a proton-transfer-reaction time-of-flight mass spectrometer (PTR-ToF-MS) to quantify the emissions of organic gases from a typical Chinese household coal stove operated with anthracite and bituminous coals. Most NMOCs (approximately 64-88%) were dominated by hydrocarbons and emitted during the ignition and flaming phases. The ratio of oxidized hydrocarbons increased during the flaming and smoldering stages due to the elevated combustion efficiency. The average emission factors of NMOCs were 121 ± 25.7 and 3690 ± 930 mg/kg for anthracite and bituminous coals, respectively. I/SVOCs contributed to approximately 30% of the total emitted NMOC mass during bituminous coal combustion, much higher than the contribution of biomass burning (approximately 1.5%). Furthermore, I/SVOCs may contribute over 70% of the secondary organic aerosol (SOA) mass formed from gaseous organic species emitted as a result of bituminous coal combustion. This study highlights the importance of inventorying coal-originated I/SVOCs when conducting SOA formation simulation studies.

14.
AAPS J ; 21(4): 71, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31161482

RESUMO

Assays for the detection and confirmation of anti-drug antibodies (ADA) are commonly used tools for assessing the immunogenicity of drug candidates in both clinical and nonclinical studies. During the development of such assays, it is typical to optimize the assay conditions based on factors such as sensitivity or signal/noise ratio (S/N) and is commonly done using an assay positive control (PC). However, even carefully optimized methods often suffer with problems due to low cut-point factors and failure to distinguish assay "noise" from a true biological response. In this paper, we describe an approach to assay development in which the impacts of assay conditions on the response and variability, both analytical and biological, of drug-naïve samples are tested by way of PC-independent assay condition optimization. Using two ADA methods as model systems, we examine the impact of minimum required dilution, assay reagent (labeled drug) concentrations, incubation time, assay, and wash buffer composition. We find that the choice of assay conditions, particularly the labeled drug concentration, can greatly affect the distribution of naïve sample responses and thus impact screening and confirmatory assay cut-points. In two case studies presented, screening assay cut-point (SCP) varied from 1.38 to 2.20 and 1.04 to 1.20 while the confirmatory assay cut-point (CCP) varied from 58.5 to 95.6% and 26.2 to 16.2% depending on the conditions tested. Some of the conditions produced unacceptably high CCP values. It is proposed that the degree of the observed impact of the assay conditions on SCP and CCP values depends on the compound nature and assay matrix composition and is likely connected with the diversity of interactions between drug protein and matrix components. Because it was also observed that higher assay SCP can associate with a loss of the PC-based assay sensitivity, additional assessment of the assay conditions would be required to determine an overall assay performance acceptability, including assay PC-based sensitivity, drug, and target tolerance characteristics. In conclusion, it is suggested that by assessing performance of treatment-naïve samples at various assay conditions, one can identify potential assay protocols that allow to avoid undesirably low screening (e.g., < 1.2) and confirmatory (e.g., < 25%) cut-points.

15.
Thorac Cancer ; 10(7): 1628-1635, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31243894

RESUMO

BACKGROUND: Microwave ablation (MWA) has recently become an established treatment option for topical therapy of lung cancer patients. In this study, we evaluated whether MWA combined with chemotherapy could improve progression-free survival (PFS) of patients with stage IV lung adenocarcinoma compared with chemotherapy alone. METHODS: A total of 49 patients were enrolled into the study; 21 patients accepted MWA therapy combined with chemotherapy, 28 patients accepted only chemotherapy. Enumeration data were analyzed using χ2 test or Fisher's exact probability test and univariate analysis was analyzed using Kaplan-Meier survival curves. Multivariate analysis was carried out with the Cox proportional hazard model. RESULTS: The treatment regimen was not correlated with clinical features of the patients, which included gender, age, smoking history, tumor site, tumor size and Eastern Cooperative Oncology Group (ECOG). The patients' 3-year overall survival (OS) was 12.5%, and median survival time was 19.3 months. The median PFS was 6.1 months and the 1-year PFS was 0.0%. The PFS was significantly associated with tumor size (P < 0.05), ECOG (P < 0.01) and treatment regimen (P < 0.01). The median time to local progression (TTLP) was 8.4 months and the 3-year TTLP was 2.0%. The TTLP was significantly associated with tumor size (P < 0.05) and treatment regimen (P < 0.01). Cox multivariate regression demonstrated that MWA combined with chemotherapy was the independent factor for both the PFS and TTLP. CONCLUSION: MWA, as a topical treatment method, when combined with chemotherapy improved the PFS and TTLP of patients with stage IV lung adenocarcinoma.

16.
Water Res ; 161: 297-307, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31203035

RESUMO

This study evaluated the anti-biofouling efficacy of capacitor mode and resistor mode in membrane distillation (MD). Polytetrafluoroethylene (PTFE) membrane coated with carbon nanotube (CNT) was adopted as the electrically conductive membrane. The biofouling formation on the pre-treatment membrane was systematically analyzed, and the results showed that both operation modes had obvious inhabitation on bacteria, especially the capacitor mode exhibited stronger prevention capability on biomass accumulation than resistor mode. NMDs analysis of microbial communities further revealed that the anti-biofouling effect mainly occurred on the membrane surface, and gram-positive biomarkers which can survive better in external electric field was distinctively found in capacitor mode through LEfSE analysis. Hypothesis was introduced to explain the anti-fouling function of two modes that in the capacitor mode, the competitive electrostatic repulsion of bacteria cells on negative electrode associated by the cell-disruption effect of electro-catalyzed reactive oxygen species (ROS) generation, while the anti-fouling function of resistor mode was a result of temperature increment on membrane surface caused by Joule heating effects. This article attempts to provide an insight of anti-fouling mechanism of electric field applied in MD and to prove the feasibility of above-mentioned operation modes as non-chemical methods for optimization of membrane-based water treatment process.

17.
J Orthop Res ; 2019 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-31254413

RESUMO

Early diagnosis and prevention of glucocorticoid (GC)-induced osteonecrosis of the femoral head (ONFH) continues to be a challenging problem for clinicians and researchers. However, the role of circulating biomarkers for GC-induced ONFH, which may reveal individual susceptibility and facilitate earlier diagnosis, remains to be determined. The aim of this study was to identify potential biomarkers that may predict early GC-induced ONFH. A total of 123 patients scheduled for initial systemic GC therapy were enrolled in this prospective nested case-control study. The serum concentrations of 13 potential biomarkers were measured in seven patients with GC-induced ONFH, diagnosed instantly after short-term use of GCs and in 20 controls who did not develop osteonecrosis despite similar GC therapy. Biomarkers were measured both before and after taking GCs to identify any differences in marker levels and the changes during GC therapy between two groups. Type I collagen cross-linked C-telopeptide (ß-CTX; p = 0.000) was significantly lower, high-density lipoprotein cholesterol (p = 0.092) and apolipoprotein (apo)-B/apo-A1 (p = 0.085) tended to be lower and higher, respectively, before GC treatment in osteonecrosis group. After GC therapy, ß-CTX (p = 0.014) was significantly lower and amino terminal telopeptide of procollagen type I (PINP; p = 0.068) tended to be lower in the osteonecrosis group. As secondary outcomes, we observed remarkable changes in nine potential biomarkers following short-term GC therapy in both groups. In conclusion, we found that ß-CTX, could potentially be used to predict GC-induced ONFH before GC therapy. Lower ß-CTX and PINP are promising biomarkers for the early diagnosis of GC-induced ONFH. These findings need to be confirmed in large-scale prospective studies.

18.
Structure ; 27(6): 883-885, 2019 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-31167123

RESUMO

Microrchidia 3 (MORC3) is an ATPase and a regulator of influenza A virus (IAVs). In this issue of Structure, Zhang et al. (2019b) solved the crystal structure of human MORC3 in complex with the IAV protein NS1, providing a mechanism for targeting MORC3 by IAVs to regulate viral infection.

19.
Rev Sci Instrum ; 90(5): 055112, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31153229

RESUMO

Several advantages are associated with an atmospheric microwave plasma torch, including low cost, easy processing, and high density of electrons and reactive species. This paper develops a compact microwave plasma torch (CMPT) operating at 2.45 GHz with an ultrabandwidth of 30 MHz. The structure and equivalent circuit of CMPT is established and analyzed based on theory of transmission line. The simulation results indicate that the resonant frequency will drift downward with the presence of plasma. In order to keep the better coupling between the microwave and plasma, a new design scheme of a fully automatic microwave plasma system is proposed. The effects of different microwave powers on the CMPT discharge are carried out on this system. Finally, in order to demonstrate the performance of the CMPT, an experiment to modify the surface of polymethyl methacrylate (PMMA) is conducted. It is found that the water contact angle is reduced from 85.4° to 32.3°, and the surface morphology characterized by atomic force microscope indicates significant improvement of the surface wettability of PMMA, which proves that the CMPT system is feasible and available.

20.
Neoplasia ; 21(6): 602-614, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31071657

RESUMO

The major role of aldehyde dehydrogenase 2 family (ALDH2) is to detoxify acetaldehyde (ACE) to non-toxic acetic acid. Many evidences suggest that ALDH2 dysfunction contributes to a variety of human diseases including cancer. However, the biological function and molecular mechanism of ALDH2 in tumor progression remain elusive. In this study, we found that ALDH2 repression was associated with poor prognosis in lung adenocarcinoma. Overexpression of ALDH2 inhibited malignant features of lung adenocarcinoma cells, such as proliferation, stemness and migration, whereas ALDH2 knockdown increased these features. Mechanistically, ALDH2 repression led to accumulation of ACE; whereas ACE enhanced the migration features of lung adenocarcinoma cells, which was associated with increased DNA damage. Importantly, accumulated ACE and increased DNA damage were identified in Aldh2-knockout (KO) mouse lung tissues in vivo. Consistent with this concept, treatment of lung adenocarcinoma cells with ALDH2 agonist Alda-1 suppressed the proliferation, stemness and migration features of lung adenocarcinoma cells. Thus, activating ALDH2, such as via its agonist, may provide a novel strategy for treatment of lung cancer.

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