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1.
Mol Med Rep ; 23(1)2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33179084

RESUMO

Lung cancer is the most prevalent and observed type of cancer in Xuanwei County, Yunnan, South China. Lung cancer in this area is called Xuanwei lung cancer. However, its pathogenesis remains largely unknown. To date, a number of studies have shown that microRNA (miR)­218 functions as a tumor suppressor in multiple types of cancer. However, the role of miR­218 and its regulatory gene network in Xuanwei lung cancer have yet to be investigated. The current study identified that the expression levels of miR­218 in XWLC­05 cells were markedly lower compared with those in immortalized lung epithelial BEAS­2B cells. The present study also demonstrated that overexpression of miR­218 could decrease cell proliferation, invasion, viability and migration in Xuanwei lung cancer cell line XWLC­05 and NSCLC cell line NCI­H157. Additionally, the results revealed that overexpression of miR­218 could induce XWLC­05 and NCI­H157 cell apoptosis by arresting the cell cycle at G2/M phase. Finally, the present study demonstrated that overexpression of miR­218 could lead to a significant increase in phosphatase and tensin homolog (PTEN) and YY1 transcription factor (YY1), and a decrease in B­cell lymphoma 2 (BCL­2) and BMI1 proto­oncogene, polycomb ring finger (BMI­1) at the mRNA and protein level in XWLC­05 and NCI­H157 cell lines. However, we did not observe any remarkable difference in the roles of miR­218 and miR­218­mediated regulation of BCL­2, BMI­1, PTEN and YY1 expression in the progression of Xuanwei lung cancer. In conclusion, miR­218 could simultaneously suppress cell proliferation and tumor invasiveness and induce cell apoptosis by increasing PTEN and YY1 expression, while decreasing BCL­2 and BMI­1 in Xuanwei lung cancer. The results demonstrated that miR­218 might serve a vital role in tumorigenesis and progression of Xuanwei lung cancer and overexpression of miR­218 may be a novel approach for the treatment of Xuanwei lung cancer.

2.
ACS Nano ; 2020 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-33152233

RESUMO

Two-dimensional (2D) materials exhibit exceptional physical and chemical properties owing to their atomically thin structures. However, it remains challenging to produce 2D materials consisting of pure monoelemental metallic atoms. Here free-standing 2D gold (Au) membranes were prepared via in situ transmission electron microscopy straining of Au films. The applied in-plane tensile strain induces an extensive amount of out-of-plane thinning deformation in a local region of an Au thin film, resulting in the nucleation and growth of a free-standing 2D Au membrane surrounded by its film matrix. This 2D membrane is shown to be one atom thick with a simple-hexagonal lattice, which forms an atomically sharp interface with the face-centered cubic lattice of the film matrix. Diffusive transport of surface atoms, in conjunction with the dynamic evolution of interface dislocations, plays important roles in the formation of 2D Au membranes during the mechanical thinning process. These results demonstrate a top-down approach to produce free-standing 2D membranes and provide a general understanding on extreme mechanical thinning of metallic films down to the single-atom-thick limit.

3.
J Hazard Mater ; : 124432, 2020 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-33189474

RESUMO

A surface defect sandwich-structural TiO2-x/ultrathin g-C3N4/TiO2-x direct Z-scheme heterojunction photocatalyst is successfully constructed. The results manifest the existence of oxygen vacancies, sandwich structure and direct Z-scheme heterojunction. Noticeably, TiO2-x/ultrathin g-C3N4/TiO2-x efficiently eliminates high toxic tetracycline hydrochloride by means of·O2-, h+ and·OH, whose removal rate is 87.7% during 90 min and the pseudo-first-order rate constant reaches up to 31.7 min-1 × 10-3. The extraordinary performance can be attributed to the special 3D structure, Z-scheme heterojunction expediting charge transfer and promoting the generation of active species, meanwhile the oxygen vacancies enhancing the spatial separation of photo-induced carriers. Moreover, various environmental factors are systematically explored by statistics. SO42-, NH3-N and pH exhibit an obvious impact on removal rate. Meanwhile, TiO2-x/ultrathin g-C3N4/TiO2-x could also effectually remove tetracycline hydrochloride from complex actual-wastewater and exhibit high stability. Besides, the photocatalytic mechanism and degradation path of tetracycline hydrochloride are also elucidated.

4.
Pathol Res Pract ; 216(12): 153257, 2020 Oct 18.
Artigo em Inglês | MEDLINE | ID: mdl-33142109

RESUMO

Glyoxalase-1 (GLO-1) is the key enzyme in aldehyde defence in cancer cells. We here evaluated the prognostic impact and association with clinico-pathological parameters and relapse-free as well as overall survival in tumor samples from 187 breast cancer patients. The determined GLO1-immunoreactive score (GLO1-IRS) did not correlate with parameters such as grading, size, hormone receptors or ki67. However, an association of GLO1-IRS with the advanced glycation end product Nε-(carboxymethyl)lysine (p = 0.07) and HER2 (p = 0.06), and a strong correlation with VEGF (p = 0.008) was found. In survival analysis, no significant impact of GLO-1 IRS could be deduced for all patients. However, GLO1-IRS correlated with treatment by radiotherapy (p = 0.008) and high GLO1-IRS predicted a shorter relapse free survival after radiotherapy (log-rank p = 0.067). METABRIC- and TCGA expression-data were analyzed for correlation of regulatory genes of the NF-κB-pathway (RELA, RELB, IRAK1), the oxidative-stress associated transcription factor nrf2 (NFE2L2), the receptor for AGEs (AGER, RAGE) as well as enzymes associated with aldehyde defense. Here, RELA, RELB and NFE2L2 correlated significantly with GLO1 expression, but there were conflicting results between the two data sources. In conclusion, GLO1 was highly expressed in cancer cells, correlated surprisingly weak with survival, but we could show a positive association with the AGE CML as well as VEGF. Gene expression data suggest a regulation of GLO-1 mRNA via both, inflammation (NF-kB) and oxidative stress (NFE2L2) in tumors.

5.
J Exp Clin Cancer Res ; 39(1): 216, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33054826

RESUMO

BACKGROUND: Long noncoding RNA (lncRNA) Linc00337 has been implicated in lung, gastric, colorectal and esophageal squamous cell carcinoma progression via various mechanisms; however, its clinicopathological significance and role in pancreatic ductal adenocarcinoma (PDAC) progression remains largely unknown. METHODS: Multiple approaches such as bioinformatic analysis, Transfection, quantitative real-time-PCR, Western blotting, animal studies, RNA-immunoprecipitation (RIP), RNA-pulldown and RNA-Fluorescence in situ hybridization (RNA-FISH) and were utilized to explore the role of Linc00337 in PDAC. RESULTS: Here we identified Linc00337 is an oncogenic lncRNA during PDAC progression. We found that the expression of Linc00337 is elevated in PDAC tissues and the higher Linc00337 predicts dismal prognosis. Functionally, Linc00337 promotes PDAC cell proliferation and cell cycle transition both in vitro and in vivo. Mechanistically, Linc00337 binds to E2F1 and functions as an E2F1 coactivator to trigger the targets expression during PDAC progression. CONCLUSION: Our results demonstrate a reciprocal regulation mechanism between Linc00337 and E2F1 in PDAC progression and report the clinical value of Linc00337 for PDAC prognosis and treatment.

6.
Int J Speech Lang Pathol ; : 1-10, 2020 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-33059481

RESUMO

PURPOSE: To fulfil the needs of assessment tools in the Chinese population, we adapted the LENA Developmental Snapshot, a parent survey that measures early language and communication development in English-speaking children. We reported the psychometric properties of the adapted questionnaire and evaluated the metric and functional equivalence between the adapted and the original instruments. METHOD: The Snapshot was translated into Chinese and reviewed by an expert panel. English-specific items (e.g. past tense, plural) were mapped onto functionally similar Mandarin vocabulary and structures. The questionnaire was administered to 1300 families with children ages 2-48 months. The Bayley Scales of Infant Development was administered to a subsample. RESULT: Scores on the adapted questionnaire showed age-related increases in the 7-36 month age range and correlated with scores on the Chinese Bayley. The questionnaire showed high internal consistency and split-half reliability. Comparison with the US norm revealed slightly lower performance in the current sample. Adaptations of English-specific items resulted in functionally equivalent targets. CONCLUSION: Despite differences in linguistic roots, the Chinese adaption of the LENA Snapshot captured developmental changes in children's language and communication abilities. Additional norming and validation efforts are needed in a more representative sample.

8.
Dalton Trans ; 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33034331

RESUMO

Non-centrosymmetric metal chalcogenides such as AgGaS2 and AgGaSe2 are two of the commercial nonlinear optical (NLO) crystals widely used in the infrared (IR) region. Nevertheless, the inherent incompatibility between the wide energy gap (Eg) and large second-harmonic generation (SHG) efficiency (dij) hinders their high-power laser applications. Recently, the development of salt-inclusion chalcogenides with non-centrosymmetric structures has attracted more and more attention and interest owing to their intensive potential applications originating from their wide Eg, strong dij, ultrahigh laser-induced damage thresholds (LIDTs) and large IR transmission range. In this frontier paper, we review the recent progress of salt-inclusion chalcogenides (including 28 related compounds) as favourable candidates for IR-NLO materials, which can be divided into 3 types according to their chemical compositions and structural characteristics: (i) the [RaXb][GanQ2n] type and its derivatives, (ii) [NaBa4Cl][Ge3S10] and its derivatives, and (iii) the [A3X][MB12(MQ4)3] type. The relationships between the non-centrosymmetric structures and NLO properties of these 3 types of compounds are summarized and briefly remarked. In addition, the present challenges of creating new IR-NLO salt-inclusion chalcogenides and future perspectives in this field are discussed.

9.
Medicine (Baltimore) ; 99(44): e22763, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-33126314

RESUMO

Ultrasound guided percutaneous thermal ablation has been well acknowledged in treating hepatic malignancy. Although thermal ablation is safe for the treatment, it may induce some lethal complications such as diaphragmatic injury, bile-stained pleural fistula, and bilious pleuritis.We presented 2 cancer patients in hepatic diaphragmatic dome showed diaphragmatic injury, bile-stained pleural fistula, and bilious pleuritis after microwave ablation (MVA). The symptoms were attenuated after chest drainage and anti-infection therapy. In the literature review, 17 articles published in the recent 10 years on diaphragmatic injury after MVA for treating hepatic cancer were available. Twenty-three cases were obtained, among which 2 showed bilious pleuritis after radiofrequency treatment. Most of the lesions were adjacent to the diaphragma. Among the articles reporting the localization of lesions, most of the cases showed lesions in S8, 2 in S7, 3 in S4, and 3 in S5, respectively. Surgical recovery was required for the patients with massive diaphragmatic injury. Only 2 cases underwent thorascopic surgery. After chest drainage and anti-infection, their symptoms were attenuated to some extent.Radiofrequency or MVA may induce pleural effusion, and special attention should be paid to the diaphragmatic injury induced by thermal ablation.


Assuntos
Diafragma/lesões , Neoplasias Hepáticas/radioterapia , Micro-Ondas/efeitos adversos , Pleurisia/etiologia , Lesões por Radiação/etiologia , Ablação por Radiofrequência/efeitos adversos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doenças Pleurais/etiologia , Fístula do Sistema Respiratório/etiologia
11.
Future Med Chem ; 12(18): 1633-1645, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32892642

RESUMO

Background: Identification of novel Ure inhibitors with high potency has received considerable attention. Methodology & results: Ure inhibition was determined using the indophenol method, the affinities to Ure were estimated via surface plasmon resonance. Seventeen new plus ten known N-monosubstituted thiosemicarbazides were synthesized and identified as novel Ure inhibitors. Out of these compounds, compound b5 shows excellent activity against both crude Ure from Helicobacter pylori (IC50 = 0.04 µM) and Ure in living cell (IC50 = 0.27 µM), with the potency being over 600-fold higher than clinical used drug acetohyroxamic acid, respectively. Surface plasmon resonance demonstrated the high affinity (Kd.#x00A0;= 6.32 nM) of b5 to Ure. Conclusion: This work provides a class of novel and promising Ure inhibitors.

12.
iScience ; 23(9): 101522, 2020 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-32932138

RESUMO

Asperuloside (ASP) is an iridoid glycoside that is extracted from Eucommia leaves. Eucommia is used in traditional Chinese medicine and has a long history of benefits on health and longevity. Here, we investigated the impact of ASP on obesity-related metabolic disorders and show that ASP reduces body weight gain, glucose intolerance, and insulin resistance effectively in mice fed with a high-fat diet (HFD). Intestinal dysbiosis is closely linked with metabolic disorders. Our data indicate that ASP achieves these benefits on metabolic homeostasis by reversing HFD-induced gut dysbiosis and by changing gut-derived secondary metabolites and metabolic signaling. Our results indicate that ASP may be used to regulate gut microbiota for the treatment of obesity and type 2 diabetes.

13.
Plant Biotechnol J ; 2020 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-32876985

RESUMO

Nitrogen (N) is one of the key essential macronutrients that affects rice growth and yield. Inorganic N fertilizers are excessively used to boost yield and generate serious collateral environmental pollution. Therefore, improving crop N use efficiency (NUE) is highly desirable and has been a major endeavour in crop improvement. However, only a few regulators have been identified that can be used to improve NUE in rice to date. Here we show that the rice NIN-like protein 4 (OsNLP4) significantly improves the rice NUE and yield. Field trials consistently showed that loss-of-OsNLP4 dramatically reduced yield and NUE compared with wild type under different N regimes. In contrast, the OsNLP4 overexpression lines remarkably increased yield by 30% and NUE by 47% under moderate N level compared with wild type. Transcriptomic analyses revealed that OsNLP4 orchestrates the expression of a majority of known N uptake, assimilation and signalling genes by directly binding to the nitrate-responsive cis-element in their promoters to regulate their expression. Moreover, overexpression of OsNLP4 can recover the phenotype of Arabidopsis nlp7 mutant and enhance its biomass. Our results demonstrate that OsNLP4 plays a pivotal role in rice NUE and sheds light on crop NUE improvement.

14.
Chem Commun (Camb) ; 56(84): 12761-12764, 2020 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-32966376

RESUMO

Here, we proposed a groundbreaking strategy for fabricating YAG:Ce3+ transparent ceramic films via a novel interface reaction of LRH nanosheets with a sapphire substrate without a tedious process. The incorporation of Gd3+ greatly enhanced the emission intensity of the ceramic film by ∼11.3 times. The prepared transparent ceramic film with a high transmittance of ∼97% is a promising photo-converter for WLEDs.

15.
Phytomedicine ; 79: 153316, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32942205

RESUMO

BACKGROUND: Alzheimer's disease (AD) is currently incurable and there is an urgent need to develop new AD drugs. Many studies have revealed the potential neuroprotective effect of Epigallocatechin-3-O-gallate (EGCG), the main antioxidant in green tea, on animal models of AD. However, a systematic review of these reports is lacking. PURPOSE: To assess the effectiveness of EGCG for AD treatment using systematic review and meta-analysis of pre-clinical trials. METHODS: We conducted a systematic search of all available randomized controlled trials (RCTs) performed up to November 2019 in the following electronic databases: ScienceDirect, Web of Science, and PubMed. 17 preclinical studies assessing the effect of EGCG on animal AD models have been identified. Meta-analysis and subgroup analysis was performed to evaluate cognition improvement of various types of AD models. The study quality was assessed using the CAMARADES checklist and the criteria of published studies. RESULTS: Our analysis shows that the methodological quality ranges from 3 to 5, with a median score of 4. According to meta-analysis of random-effects method, EGCG showed a positive effect in AD with shorter escape latency (SMD= -9.24, 95%CI= -12.05 to -6.42) and decreased Aß42 level (SD= -25.74,95%CI= -42.36 to -9.11). Regulation of α-, ß-, γ-secretase activity, inhibition of tau phosphorylation, anti-oxidation, anti-inflammation, anti-apoptosis, and inhibition of AchE activity are reported as the main neuroprotective mechanisms. Though more than 100 clinical trials have been registered on the ClinicalTrials.gov, only one clinical trial has been conducted to test the therapeutic effects of EGCG on the AD progression and cognitive performance. CONCLUSION: Here, we conducted this review to systematically describe the therapeutic potential of EGCG in animal models of AD and hope to provide a more comprehensive assessment of the effects in order to design future clinical trials. Besides, the safety, blood-brain barrier (BBB) penetration and bioavailability issues in conducting clinical trials were also discussed.

16.
Artigo em Inglês | MEDLINE | ID: mdl-32805976

RESUMO

Electrochemical CO2 reduction represents a sustainable approach for the conversion of CO2 into valuable fuels and chemicals. Here, we fabricated a series of composite nanomaterials through template-oriented polymerization of covalent organic frameworks (COFs) with isolated cobalt porphyrin units on amino-functionalized carbon nanotubes (CNTs) for efficiently electrocatalytic CO2 reduction reaction (CO2RR). Compared with pure COFs, the hybrid form of ultrathin COF nanolayers wrapped on the conductive scaffold leads to distended current density and stable Faradaic efficiency (FE) for CO2-to-CO conversion over a wide potential range. Specifically, the catalytic performances of the system can be finely optimized by the modification of the reticular structure with different functional groups. Our work gives a new strategy for the preparation of highly active and selective electrocatalysts for CO2RR.

17.
J Contam Hydrol ; 234: 103689, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32836106

RESUMO

An accurate solute transport model is critical to the interpretation of single-well push-pull (SWPP) test. Previous studies of SWPP test generally consider solitary aquifer that is confined by impermeable layers. Also, existing solutions for solute transport in aquifer-aquitard systems only consider the injection phase and over-simplify the flow field by assuming uniformly distributed velocity in the aquitard. In this study, we developed a numerical model with Dirichlet boundary condition for SWPP test affected by leakage described by a low-permeability non-Darcian expression involving a threshold pressure gradient (I0). Our SWPP test model considered transient flow in multi-phases, which include injection, chase, rest and extraction phases. Finite-difference scheme was adopted to solve the models of flow and solute transport. The results indicate that an increasing hydraulic diffusivity leads to a greater peak value of breakthrough curve (BTC) while a medium with larger grain size results in less estimation error when using steady-state flow model to interpret the transient SWPP test. Additionally, A greater I0 makes the solute stored in aquitard more difficult to be extracted out due to dispersion dominance, which results in higher BTC values. For the purpose of application, a lumped dimensionless index called the non-Darcian index (NDI) was proposed to quantify the overestimation degree by neglecting leakage, and the underestimation degree by accounting for Darcian leakage, when interpreting the SWPP test with low-permeability non-Darcian leakage. The long-term slope of breakthrough curve coupled with the NDI can be employed to determine the cases in which the low-permeability non-Darcian leakage should be considered.

18.
Clin Rheumatol ; 39(10): 3159, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32757077

RESUMO

In the original version of the above article the References 19 and 20 were incorrect which cannot describe the development of the SPARCC score.

19.
Immunopharmacol Immunotoxicol ; : 1-11, 2020 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-32811237

RESUMO

METHODS: 36 female BALB/c mice were selected and randomly divided the mice into four groups. We established a BALB/c mouse model of TCE sensitization and pretreatment with GdCl3 (40 mg/kg) by intraperitoneal injection during the during the 17th and 19th days. RESULTS: We found F4/80, the marker of Kupffer cell, was increased in TCE positive group. GdCl3 treatment successfully blocked the activation of Kupffer cell. TNF-α was increased significantly in liver of TCE sensitized mice and decreased significantly when low-dose GdCl3 was used. We found TNF receptor 1 (TNFR1) was increased significantly and GdCl3 treatment resumed the expression of TNFR1 to normal level, as well as the F4/80, TNF-α and TNFR1 mRNA. We also found both caspase-8 and caspase-3 increased in TCE positive group and decreased in TCE + GdCl3 positive group. The number of apoptotic cells in TCE sensitized mice increased by TUNEL staining, and GdCl3 treatment alleviated this increase. Some cells showed edema and inflammatory cell aggregation in liver of TCE positive group, while in the TCE + GdCl3 positive group, the cytoplasm became loose and vacuole-like degeneration occurred. CONCLUSION: Our study unveils cross-talk between Kupffer cell activation and TNFR1 which mediate apoptosis in liver of TCE sensitized mice.

20.
Int Immunopharmacol ; 88: 106897, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32822909

RESUMO

Trichloroethylene (TCE) induced TCE hypersensitivity syndrome which makes immune injuries in multi-system. The multiple organ damage included skin, liver, kidney and so on. The main manifestations of liver injuries were apoptosis and edema of hepatocytes. In our previous research, we found the activation of Kupffer cells (KCs) which increased IL-6 can aggravate liver cell apoptosis in TCE sensitized mice. However, the mechanism of IL-6 in liver damages induced by TCE was not clear. This study explored the function of IL-6/STAT3 signal pathway on the TCE induced apoptosis of liver cell. We established a TCE sensitized BALB/c mouse model with a KCs inhibitor GdCl3, we found that the expressions of ALT and AST in TCE sensitization positive mice were higher than other mice, and the expressions of apoptosis-related proteins were up-regulated in TCE sensitization positive mice, GdCl3 could alleviate this process. Meanwhile, GdCl3 could significantly decrease the expressions of IL-6/STAT3 proteins. All in all, the activation of KCs can increase the expression of IL-6, IL-6R and phosphorylate STAT3, induces hepatocyte apoptosis, and participates in immunity damage of liver which induced by TCE.

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