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1.
Sci Total Environ ; 804: 149965, 2021 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-34508937

RESUMO

Ammonium removal in wastewater treatment plants demands large quantities energy input, such as aeration for wastewater and the addition of organics for nitrate reduction. Anaerobic ammonium oxidation coupled to Fe(III) reduction, called Feammox process play a crucial role in natural nitrogen cycle, which has been rarely investigated in the field of wastewater treatment. Besides, Iron-reducing bacteria (FeRB) as function bacteria of Feammox could transfer electrons to iron oxide by oxidizing organics. The possibility of anaerobic ammonium removal coupled with organics should be investigated to assess the potential of Feammox process for conventional wastewater treatment. In this study, five Fe(III) compounds, Fe2O3, Fe3O4, Fe(OH)3, Citrate-Fe and pyrite were supplemented to investigate the effect of iron oxides on ammonium removal in serum bottles with working volume of 100 mL. It was found that ammonium removal efficiency of the Fe2O3 group was the highest. To simulate wastewater treatment process in sewage treatment plant, three Up-flow anaerobic sludge blanket reactors with volume of 250 mL adding Fe2O3 were applied with influent of ammonium and carbon sources. It was found that the organics significantly inhibited the ammonium removal by Feammox process. This was attributed to that carbon sources and ammonium could be used as electron donors for Fe(III) reduction. In addition, this nitrogen removal was also likely related with the iron cycle, i.e., Fe(III) reduction with ammonium oxidation and Fe(II) oxidation with nitrate/nitrite reduction. This study provides a promising alternative technology for anaerobic ammonium removal in wastewater treatment. Optimizing nitrogen removal and carbon sources applied in conventional wastewater plants are required in future.

2.
Environ Res ; 204(Pt A): 111995, 2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34492278

RESUMO

Due to the potential hazard of perfluorooctanoic acid (PFOA), hexafluoropropylene oxide dimer acid (HFPO-DA, GenX) has become a typical alternative since 2009. However, GenX has recently been reported to have equal or even greater toxicity and bioaccumulation than PFOA. Considering the suitability of alternatives, it is quite essential to study and compare the degradation degree between PFOA and GenX in water. Therefore, in the present study, a comprehensive degradation comparison between them via electrooxidation with a titanium suboxide membrane anode was conducted. The degradation rate decreased throughout for PFOA, while it first increased and then decreased for GenX when the permeate flux increased from 17.3 L to 100.3 L m-2·h-1. The different responses of PFOA and GenX to flux might be attributed to their different solubilities. In addition, the higher kobs of PFOA demonstrated that it had a better degradability than GenX by 2.4-fold in a mixed solution. The fluorinated byproduct perfluoropropanoic acid (PFPrA) was detected as a GenX intermediate, suggesting that ether bridge splitting was needed for GenX electrooxidation. This study provides a reference for assessing the degradability of GenX and PFOA and indicates that it is worth reconsidering whether GenX is a suitable alternative for PFOA from the point of view of environmental protection.

3.
Plant Physiol Biochem ; 166: 495-504, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34166976

RESUMO

Flavonoid glycosides are important plant secondary metabolites with broad pharmacological activities. Flavonoid glycosides are generated from aglycones, in reactions catalyzed by typical uridine diphosphate-dependent glycosyltransferases (UGTs). Liverworts produce various types of flavonoid glycosides; however, only two UGTs have been characterized from liverworts to date. Here, we isolated three genes encoding UGTs (MeUGT1, MeUGT2, and MpalUGT1) from the liverwort species Marchantia emarginata and Marchantia paleacea through transcriptome sequencing. Recombinant MeUGT1, MeUGT2, and MpalUGT1 proteins heterologously produced in Escherichia coli exhibited catalytic activity towards multiple flavonoids. MeUGT1 and MpalUGT1 catalyzed the glycosylation of flavonols into the corresponding 3-O-glucosides with UDP-glucose as the sugar donor, while MeUGT2 exhibited a wider substrate specificity that included flavonols, flavones, and flavanones. When MeUGT2 was expressed in E. coli, the yield of flavonol 3-O-glucosides reached to 40-60% with feeding of the substrates kaempferol or quercetin under optimal conditions. Furthermore, heterologous expression of MeUGT1 in Arabidopsis thaliana increased the flavonol glycoside contents in the plants. Therefore, the UGTs characterized in this study could provide new data that will be useful for examining flavonoid biosynthesis in liverworts.


Assuntos
Glucosiltransferases , Marchantia , Clonagem Molecular , Escherichia coli/genética , Escherichia coli/metabolismo , Flavonoides , Glucosiltransferases/genética , Glucosiltransferases/metabolismo , Marchantia/genética , Marchantia/metabolismo , Especificidade por Substrato
4.
Sci Total Environ ; 773: 145706, 2021 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-33940765

RESUMO

The hydrophilic biomaterial was constructed based on the soluble extracellular polysaccharides (s-EPS) secreted by Bacillus megaterium and zeolitic imidazolate framework-8 (ZIF-8), namely ZIF-8@s-EPS, wrapped in s-EPS shell with ZIF-8 as the core. ZIF-8@s-EPS was used as a novel multifunctional biomaterial in water treatment for the first time. Unexpectedly, results showed ZIF-8@s-EPS with strong synergistic effect presented multifunctional performances including descaling, antifouling and antibacterial. Scale inhibition efficiency reached 98.63% for CaCO3 and as high as 99.40% for CaSO4 at concentration 20.00 mg/L. The synergy of s-EPS and ZIF-8 demonstrated effective antibacterial activity against Pseudomonas aeruginosa and inhibitory effect on biofilms, and result presented that ZIF-8@s-EPS could inhibit the growth of nearly 89.4% P. aeruginosa. Therefore, the obtained insights will shed light on the development of s-EPS modified biomaterials in water treatment.


Assuntos
Purificação da Água , Zeolitas , Antibacterianos/farmacologia , Materiais Biocompatíveis , Imidazóis , Polissacarídeos
5.
J Hazard Mater ; 412: 125248, 2021 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-33951868

RESUMO

Cadmium (Cd) is a typical and widely present toxic heavy metals in environments. Biomineralization of Cd ions could alleviate the toxicity and produce valuable products in certain waste streams containing selenite. However, the impact of the intrinsic Cd(II) efflux system on the biotransformation process remains unrevealed. In this work, the significance of the efflux system on Cd biomineralization was evaluated by constructing engineered Escherichia coli strains, including ΔzntA with suppressed Cd(II) efflux system and pYYDT-zntA with strengthened Cd(II) efflux system. Compared to the wild type (WT), 20% more Cd ions were accumulated in ΔzntA and 17% less were observed in pYYDT-zntA in the presence of selenite as determined by inductively coupled plasma atomic emission spectrometer. Through combination with X-ray absorption fine structure analysis, it was discovered that 50% higher production of CdSxSe1-x quantum dots (QDs) was achieved in the ΔzntA cells than that in the WT cells. Moreover, the ΔzntA cells exhibited the same viability as the WT cells and the pYYDT-zntA cells because accumulated Cd ions were transformed into biocompatible QDs. In addition, the biosynthesized QDs had a uniform particle size (3.82 ± 0.53 nm) and a long fluorescence lifetime (45.6 ns), which could potentially be utilized for bio-imaging. These results not only elucidate the significance of Cd(II) efflux system in the biotransformation of Cd ions and selenite, but also provide a promising way to recover Cd and Se as valuable products in certain waste streams.


Assuntos
Proteínas de Transporte de Cátions , Pontos Quânticos , Biomineralização , Cádmio/metabolismo , Cádmio/toxicidade , Cátions , Escherichia coli/genética , Escherichia coli/metabolismo
6.
Curr Med Sci ; 41(2): 390-397, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33877558

RESUMO

The features and treatment of 98 Chinese patients with immunoglobulin G4 (IgG4)-related disease (IgG4-RD) referred to a single tertiary referring centre were reviewed. Patients diagnosed with IgG4-RD according to the comprehensive diagnostic criteria (CDC) were included in the retrospective study from May 2012 to March 2019. We collected data on clinical, laboratory, imaging, histological features and treatment. Totally, 98 patients with IgG4-RD were enrolled. The common clinical manifestations included abdominal pain, salivary gland swelling and lymphadenopathy. 51% of the patients had multiple organs involvement. Lymph nodes, pancreas and salivary glands were most commonly involved. Four rare sites including ulna, cerebellum, scalp, and mammary gland were found. The serum IgG4 level was increased by 85.7%. The serum IgG4 level was positively correlated with the number of involved organs, IgG and IgG4/IgG. Low C3 and C4 levels were observed in 37.5% and 12.2% patients respectively, and all patients with kidney involvement had hypocomplementemia. A total of 54 patients underwent tissue biopsies, and 55.6%, 31.5% and 11.1% cases were diagnosed as definite, probable and possible IgG4-RD, respectively. Eighty-eight patients received glucocorticoids (GCs) therapy. Five patients underwent radical surgery to remove the lesion. 73% of them presented a complete or partial remission. IgG4-RD is a systemic fibroinflammatory disease with involvement of multiple organs throughout the body including some rare sites. Most IgG4-RD patients had increased serum IgG4 levels and patients with kidney involvement showed hypocomplementemia. GCs therapy is effective. More research is needed to provide a more reliable basis for the diagnosis and treatment of patients.

7.
BMC Pediatr ; 21(1): 126, 2021 03 15.
Artigo em Inglês | MEDLINE | ID: mdl-33722205

RESUMO

BACKGROUND: Phenylketonuria (PKU) is a genetic metabolic disorder in which patients have no ability to convert phenylalanine to tyrosine. Several autoimmune diseases have been reported to combine with PKU, co-existent of PKU and Juvenile Idiopathic Arthritis (JIA) has not been presented. CASE PRESENTATION: The girl was diagnosed with PKU at the age of 1 month confirmed by molecular data. At the age of 3.5 years, she presented with pain and swelling of her right ankle, right knee, and right hip joint. After a serial of examinations, she was diagnosed with JIA and treated with a nonsteroidal anti-inflammatory drug. CONCLUSIONS: We report a rare case of a 4-year-old girl with PKU and JIA, which supports a possible interaction between PKU and JIA. Long-term metabolic disturbance may increase the susceptibility to JIA. Further chronic inflammation could alter the metabolism of tryptophan and tyrosine to increase blood Phe concentration. In addition, corticosteroid and methotrexate therapy for JIA may increase blood Phe concentration.


Assuntos
Artrite Juvenil , Fenilcetonúrias , Corticosteroides/uso terapêutico , Anti-Inflamatórios não Esteroides/uso terapêutico , Artrite Juvenil/complicações , Artrite Juvenil/diagnóstico , Artrite Juvenil/tratamento farmacológico , Pré-Escolar , Feminino , Humanos , Fenilalanina , Fenilcetonúrias/complicações , Fenilcetonúrias/diagnóstico
8.
Biomed Res Int ; 2021: 8858326, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33728343

RESUMO

Long noncoding RNAs (lncRNAs) are a class of important regulators participating in various pathological processes. Until now, the role of lncRNAs in the occurrence and development of intrahepatic cholestasis of pregnancy (ICP) has rarely been investigated. The data from microarray screening revealed 58 upregulated and 85 downregulated lncRNAs and 47 upregulated and 71 downregulated mRNAs in ICP patients compared to healthy controls. Bioinformatics analysis revealed biological processes focused on lipid metabolism, apoptosis, cell cycle, cell differentiation, and oxidative stress. Furthermore, the expressions of three lncRNAs (ENST00000505175.1, ASO3480, and ENST00000449605.1) chosen for verification were significantly decreased and showed the diagnostic and prognostic value for ICP based on ROC analysis. This is the first study to report the specific role of lncRNAs in ICP, which may be helpful for the diagnosis and prognosis of ICP clinically.


Assuntos
Colestase Intra-Hepática , Perfilação da Expressão Gênica , Complicações na Gravidez , RNA Longo não Codificante/biossíntese , Adulto , Colestase Intra-Hepática/diagnóstico , Colestase Intra-Hepática/metabolismo , Feminino , Humanos , Gravidez , Complicações na Gravidez/diagnóstico , Complicações na Gravidez/metabolismo , Prognóstico
9.
Epilepsy Behav ; 116: 107770, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33556864

RESUMO

OBJECTIVE: A number of studies have suggested a pathophysiological link between allergic diseases and epilepsy. Understanding the association between allergic diseases and epilepsy can help establish healthcare policies, implement prevention strategies, and provide a new direction for treatment. The study aimed to examine the association between allergic diseases and epilepsy. METHODS: PubMed, EMBASE, and Web of Science were searched for relevant primary articles. Two individuals independently conducted abstract screening, full-text review, data extraction, and quality assessment. Random-effects models were used to pool the risk estimates. RESULTS: From the 3124 citations identified, 32 were reviewed in full text. Finally, 11 studies with a total of 3,312,033 subjects were eligible for the analyses. Few studies reported the type of epilepsy, and there were inconsistent attempts to control for confounding. The pooled result showed that there was an 81% increase in the prevalence of epilepsy among individuals with asthma compared with those without asthma (odds ratio: 1.81, 95% confidence interval [CI]:1.47-2.21). The incidence of epilepsy in patients with eczema was 2.57 (95%CI: 1.54-4.27). Sensitivity analyses confirmed that no single study qualitatively influenced the pooled OR. All funnel plots were asymmetric upon visual inspection, suggesting publication bias. CONCLUSION: Our findings suggest that patients with allergic diseases might have a high risk of epilepsy. Additional high-quality primary studies are required to confirm the association, obtain information regarding the mechanism of association, and determine prevention opportunities.


Assuntos
Epilepsia , Epilepsia/complicações , Epilepsia/epidemiologia , Humanos , Incidência , Razão de Chances , Prevalência
10.
J Hazard Mater ; 408: 124416, 2021 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-33158650

RESUMO

Bioelectrochemical systems (BESs) have been known as a promising technology for accelerating aromatic contaminants degradation and energy recovery. However, most existing studies concentrate on aromatics metabolized through a benzoyl-CoA pathway while those metabolized through other pathways are limited. In this work, resorcinol, a typical aromatic contaminant as well as a key central intermediate (other than benzoyl-CoA) involved in aromatics anaerobic biodegradation, was studied in BESs. Unlike the general impression of the relatively poor organic-to-current performance in the aromatics driven BESs, high efficiencies for resorcinol-fed BESs were observed with a current density and coulombic efficiency of up to 0.26 ± 0.05 mAcm-2 and 74.3 ± 10.7%, respectively. The higher performance likely correlates to the readily fermentable property of resorcinol. Analysis of microbial communities in the biofilm suggests a syntrophic interaction between resorcinol-degrading bacteria (RDB) and anode-respiring bacteria (ARB) was involved in current generation. Additional tests involving the removal of accumulated acetate through fast resorcinol feeding indicates that a mechanism based on direct utilization of resorcinol for current generation may also exist. This study extends the knowledge for the fate of aromatics in BESs and indicates that aromatics entering into the resorcinol metabolic pathway can be treated efficiently with good energy recovery efficiency in BESs.


Assuntos
Antagonistas de Receptores de Angiotensina , Fontes de Energia Bioelétrica , Inibidores da Enzima Conversora de Angiotensina , Biodegradação Ambiental , Eletrodos , Elétrons , Resorcinóis
11.
Sci Total Environ ; 751: 141703, 2021 Jan 10.
Artigo em Inglês | MEDLINE | ID: mdl-32882553

RESUMO

Ammonium removal in wastewater treatment plants requires a large number of energy input, such as aeration and the addition of organics. Alternative, more economical technologies for nitrogen removal from wastewater are required. This study comprehensively investigated the feasible of microbial electricity coupled with Fe(III) reduction promoting the anaerobic ammonium removal. It was found that electrostimulation coupled with Fe(III) reduction (bioelectrochemical systems-Fe(III) (BES-Fe(III)) reactor) enhanced the anaerobic ammonium removal by 50.38% and 38.8% compared with the BES reactor and Fe(III) reactor, respectively. The ammonium removal rate reached the highest value of 80.62 ± 0.26 g N m-3·d-1 in the Fe(III)-BES reactor comparable to conventional wastewater treatment plants (WWWTPs). The improvement of ammonium removal might be the synergistic effect of BES and Feammox process on reaction process and microorganisms. Firstly, the addition of Fe2O3 could improve the electrochemical characteristics by enriching iron-reducing bacterial (FeRB). Secondly, the improved ammonium removal could be due to nitrite generated from Feammox process driving the anodic ammonium oxidation. Additionally, the ammonium removal improvement might be the effect of BES on the Fe2+ leaching so as to accelerate the Fe (II)/Fe(III) cycle. In agreement, higher abundance of FeRB and iron-oxidizing bacteria was detected in the Fe(III)-BES reactor. This study provides a lower energy consumption and operational cost technology compared with the conventional partial nitrification/denitrification, which was more than 800 times less than for the conventional wastewater treatment.


Assuntos
Compostos de Amônio , Terapia por Estimulação Elétrica , Anaerobiose , Reatores Biológicos , Desnitrificação , Compostos Férricos , Nitrogênio/análise , Oxirredução , Águas Residuárias
12.
Plant Physiol ; 184(4): 1731-1743, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33023939

RESUMO

During the course of evolution of land plants, different classes of flavonoids, including flavonols and anthocyanins, sequentially emerged, facilitating adaptation to the harsh terrestrial environment. Flavanone 3ß-hydroxylase (F3H), an enzyme functioning in flavonol and anthocyanin biosynthesis and a member of the 2-oxoglutarate-dependent dioxygenase (2-ODD) family, catalyzes the hydroxylation of (2S)-flavanones to dihydroflavonols, but its origin and evolution remain elusive. Here, we demonstrate that functional flavone synthase Is (FNS Is) are widely distributed in the primitive land plants liverworts and evolutionarily connected to seed plant F3Hs. We identified and characterized a set of 2-ODD enzymes from several liverwort species and plants in various evolutionary clades of the plant kingdom. The bifunctional enzyme FNS I/F2H emerged in liverworts, and FNS I/F3H evolved in Physcomitrium (Physcomitrella) patens and Selaginella moellendorffii, suggesting that they represent the functional transition forms between canonical FNS Is and F3Hs. The functional transition from FNS Is to F3Hs provides a molecular basis for the chemical evolution of flavones to flavonols and anthocyanins, which contributes to the acquisition of a broader spectrum of flavonoids in seed plants and facilitates their adaptation to the terrestrial ecosystem.

13.
Plant Sci ; 299: 110577, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32900434

RESUMO

Flavonoid glucosides, typically generated from aglycones via the action of uridine diphosphate-dependent glycosyltransferases (UGTs), both contribute to plant viability and are pharmacologically active. The properties of UGTs produced by liverworts, one of the basal groups of non-vascular land plants, have not been systematically explored. Here, two UGTs potentially involved in flavonoids synthesis were identified from the transcriptome of Plagiochasma appendiculatum. Enzymatic analysis showed that PaUGT1 and PaUGT2 accepted various flavones, flavonols, flavanones and dihydrochalcones as substrates. A mutated form PaUGT1-Q19A exhibited a higher catalytic efficiency than did the wild type enzyme. When expressed in Escherichia coli, the yield of flavonol 7-O-glucosides reached to over 70 %. Co-expression of PaUGT1-Q19A with the upstream flavone synthase I PaFNS I-1 proved able to convert the flavanone aglycones naringenin and eriodictyol into the higher-yield apigenin 7-O-glucoside (A7G) and luteolin 7-O-glucoside (L7G). The maximum concentration of 81.0 µM A7G and 88.6 µM L7G was achieved upon supplementation with 100 µM naringenin and 100 µM eriodictyol under optimized conditions. This is the first time that flavonoids UGTs have been characterized from liverworts and co-expression of UGTs and FNS Is from the same species serves as an effective strategy to synthesize flavone 7-O-glucosides in E. coli.


Assuntos
Glucosídeos/biossíntese , Glicosiltransferases/genética , Hepatófitas/genética , Proteínas de Plantas/genética , Flavonoides/metabolismo , Glucosídeos/economia , Glicosiltransferases/metabolismo , Hepatófitas/enzimologia , Hepatófitas/metabolismo , Filogenia , Proteínas de Plantas/metabolismo
14.
Environ Sci Technol ; 54(17): 10713-10721, 2020 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-32786571

RESUMO

Biotransformation of selenite to valuable elemental selenium nanoparticles (Se0) is a promising avenue to remediate seleniferous environments and simultaneously recover selenium (Se). However, the underlying oxyanion competition and selenite transformation mechanism in prokaryotes are poorly understood. In this work, the impacts of phosphate on selenite uptake and transformation were elucidated with Escherichia coli and its mutant deficient in phosphate transport as model microbial strains. Selenite uptake was inhibited by phosphate in E. coli. Moreover, the transformation of internalized Se was shifted from Se0 to toxic organo-Se with elevated phosphate levels, as evidenced by the linear combination fit analysis of the Se K-edge X-ray absorption near-edge structure. Such a phosphate-regulated selenite biotransformation process was mainly assigned to the competitive uptake of phosphate and selenite, which was primarily mediated by a low affinity phosphate transporter (PitA). Under phosphate-deficient conditions, the cells not only produced abundant Se0 nanoparticles but also maintained good cell viability. These findings provide new insights into the phosphate-regulated selenite biotransformation by prokaryotes and contribute to the development of new processes for bioremediating Se-contaminated environments, as well as bioassembly of Se0.


Assuntos
Ácido Selenioso , Selênio , Biotransformação , Escherichia coli , Fosfatos , Selenito de Sódio
15.
Plant Physiol Biochem ; 155: 716-724, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32862021

RESUMO

Typical plant terpene synthases (TPSs) are responsible for the production of terpenes, a major class of plant secondary metabolites. However, various nonseed plants also harbor genes encoding microbial terpene synthase-like (MTPSL) enzymes. Here, a scan of 31 ferns transcriptomes revealed 40 sequences putatively encoding MTPSLs. Two groups of sequences were recognized based on the key conserved motifs. Four representative genes were isolated from each of the four species Adiantum capillus-veneris, Cyclosorus parasiticus, Drynaria bonii and Microlepia platyphylla. Following their heterologous expression in E. coli, the recombinant proteins were tested for monoterpene synthase and sesquiterpene synthase activity. These enzymatic products were typical monoterpenes and sesquiterpenes that have been previous shown to be generated by classical plant TPSs when provided with GPP and FPP as substrates. Subcellular localization experiments in the leaf epidermis of Nicotiana benthamiana and onion (Allium cepa) inner epidermal cells indicated that AcMTPSL1 and DbMTPSL were deposited in both the cytoplasm and nucleus, whereas CpMTPSL1 and MpMTPSL were localized in the cytoplasm, chloroplasts and nucleus. AcMTPSL1 was up-regulated in plants exposed to methyl jasmonate treatment, suggesting a role for this gene in host defense. This study provides more information about the catalytic function of MTPSLs in nonseed plants and for the first time, the subcellular localization of MTPSLs was experimentally characterized.


Assuntos
Alquil e Aril Transferases/genética , Gleiquênias/enzimologia , Escherichia coli , Gleiquênias/genética , Proteínas de Plantas/genética , Terpenos , Transcriptoma
16.
J Vet Sci ; 21(3): e45, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32476319

RESUMO

BACKGROUND: Feline mammary carcinoma is the third most common cancer that affects female cats. OBJECTIVES: The purpose of this study was to screen differential serum proteins in feline and clarify the relationship between them and the occurrence of feline mammary carcinoma. METHODS: Chinese pastoral cats were used as experimental animals. Six serum samples from cats with mammary carcinoma (group T) and six serum samples from healthy cats (group C) were selected. Differential protein analysis was performed using a Label-free technique, while parallel reaction monitoring (PRM) was performed to verify the screened differential proteins. RESULTS: A total of 82 differential proteins were detected between group T and group C, of which 55 proteins were down regulated and 27 proteins were up regulated. Apolipoprotein A-I, Apolipoprotein A-II (ApoA-II), Apolipoprotein B (ApoB), Apolipoprotein C-III (ApoC-III), coagulation factor V, coagulation factor X, C1q, albumen (ALB) were all associated with the occurrence of feline mammary carcinoma. Differential proteins were involved in a total of 40 signaling pathways, among which the metabolic pathways associated with feline mammary carcinoma were the complement and coagulation cascade and cholesterol metabolism. According to the Label-free results, ApoB, ApoC-III, ApoA-II, FN1, an uncharacterized protein, and ALB were selected for PRM target verification. The results were consistent with the trend of the label-free. CONCLUSIONS: This experimen is the first to confirm ApoA-II and ApoB maybe new feline mammary carcinoma biomarkers and to analyze their mechanisms in the development of such carcinoma in feline.


Assuntos
Proteínas Sanguíneas/análise , Carcinoma/veterinária , Doenças do Gato/sangue , Neoplasias Mamárias Animais/sangue , Proteoma/análise , Animais , Carcinoma/sangue , Gatos , Feminino , Proteômica , Soro/química
17.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 51(3): 428-433, 2020 May.
Artigo em Chinês | MEDLINE | ID: mdl-32543155

RESUMO

Objective: To analyze the effects of biofeedback combined with task-oriented training on hand function, Gesell's infant development scale score (Gesell) and balance ability in children with spastic cerebral palsy (SCP). Methods: 66 children with SCP admitted to our hospital from January 2016 to June 2018 were randomly divided into the control group and the observation group. The control group ( n=33) received conventional rehabilitation treatment, and the observation group ( n=33) received biofeedback combined with task-oriented training based on the treatment of control group. After 6-month treatment, Modified Ashworth scale (MAS) score, Berg balance scale (BBS) score, standing and walking function score in gross motor function scale (GMFM), assisting hand assessment scales (AHA) score, Gesell scale score and satisfaction of the children's parents were compared between the two groups. Results: The MAS score after treatment was lower than that before treatment in both two groups ( P<0.05), and the BBS score after treatment was higher than that before treatment in both two groups ( P<0.05). After treatment, the MAS score in the observation group was lower than the control group, and the BBS score in the observation group was higher than the control group ( P<0.05). The scores of standing and walking function after treatment were higher than that before treatment in both two groups ( P<0.05). After treatment, the scores of standing and walking function in the observation group were higher than the control group ( P<0.05). The AHA score and Gesell developmental quotient (DQ) score after treatment were higher than that before treatment in both two groups ( P<0.05). After the treatment, the AHA score and Gesell DQ score in the observation group were higher than the control group ( P<0.05). The satisfaction rate of rehabilitation treatment in the observation group was higher than the control group (90.91% vs. 60.61%, P<0.05). Conclusion: Biofeedback combined with task-oriented training can improve balance ability, spasm relieve, hand function, development level, standing and walking function in the children with spastic cerebral palsy and increase the treatment satisfaction degree of children's guardians.


Assuntos
Terapia por Acupuntura , Biorretroalimentação Psicológica , Paralisia Cerebral , Paralisia Cerebral/terapia , Criança , Humanos , Equilíbrio Postural , Posição Ortostática , Análise e Desempenho de Tarefas , Caminhada
18.
In Vitro Cell Dev Biol Anim ; 56(4): 349-357, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32415544

RESUMO

Ischemic injury in the heart is associated with low oxygen, leading to the damage of cardiomyocytes. The lncRNA-XIST is known to involve in post-ischemia myocardial remodeling. However, the roles and mechanism of XIST in the hypoxia-induced cardiomyocyte are still under investigation. Moreover, studies that elucidated the impaired glucose metabolism present new hallmark of ischemic cardiovascular injury. The objective of this study is to investigate the effects of lncRNA-XIST on cardiomyocyte injury under hypoxia. Here, we demonstrate that the XIST expressions of cardiomyocyte line, H9c2 were apparently suppressed by long-time hypoxia exposure under low glucose supply. On the contrary, miRNA-125b showed reverse expression pattern to XIST. We identified that XIST functioned as a ceRNA of miR-125b to downregulate its expression in both cell line and rat primary cardiomyocyte. Under low glucose supply, H9c2 cells exhibited increased susceptibility to hypoxia. We observed overexpression of XIST significantly elevated glycose metabolism rate under hypoxia, but overexpression of miR-125b inhibited glycose metabolism rate of cardiomyocyte under hypoxia. The glycolysis enzyme, hexokinase 2 (HK2) was validated as a direct target of miR-125b, which binds to the 3'-UTR region of HK2 mRNA in cardiomyocytes. Moreover, inhibition of miR-125b significantly protected the hypoxia-induced cardiomyocyte injury through restoration of glucose metabolism. Finally, we demonstrated that transfection of miR-125b in lncRNA-XIST overexpressed H9c2 cells effectively abolished the XIST-activated glucose metabolism and cardiomyocyte protection under hypoxia. The present study illustrates roles of the XIST-miR-125b-HK2 axis in the hypoxia-induced cardiomyocyte injury and proposes that maintaining glucose metabolism might be an effective approach for protection of cardiomyocyte injury.


Assuntos
Hexoquinase/metabolismo , MicroRNAs/metabolismo , Miócitos Cardíacos/metabolismo , Miócitos Cardíacos/patologia , RNA Longo não Codificante/metabolismo , Transdução de Sinais , Animais , Sequência de Bases , Hipóxia Celular/genética , Linhagem Celular , Citoproteção , Glucose/metabolismo , Glicólise , MicroRNAs/genética , RNA Longo não Codificante/genética , Ratos
19.
Environ Res ; 184: 109317, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32145551

RESUMO

Biosynthesized noble metal nanoparticles (NPs) as promising green catalysts for electrochemical application has invited a lot of attention. However, effective electron transfer between biosynthesized NPs and electrode remains a challenge due to the uncontrollable and poor conductive property of cell substrates. In this study, graphene oxide (GO) was introduced into a bio-Pd synthesis process governed by Shewanella oneidensis MR-1, which was demonstrated to be simultaneously reduced with Pd(II) and transformed to reduced GO (rGO), resulting in the formation of a Pd-cells-rGO composite. Compared to the control without rGO (Pd-cells), the electrochemical conductivity of Pd-cells-rGO composite increased from almost zero to 196 µS cm-1, indicating the rGO facilities the electron transport across the composite. Electrochemical characterizations revealed the electrochemical active surface area (ECSA) of Pd in Pd-cells-rGO was enlarged by increasing the amount of rGO in the composite, clearly indicating that the conductive network created by rGO enable the Pd NPs receive electrons from electrode and become electrochemical active. A considerable enhancement of electrocatalytic activity was further confirmed for Pd-cells-rGO as indicated by 36.7- and 17.2-fold increase (Pd-cells-rGO with Pd/GO ratio of 5/1 vs Pd-cells) of steady state current density toward hydrogen evolution and nitrobenzene reduction at -0.7 V and -0.55 V vs Ag/AgCl, respectively. We also compared the electrocatalytic performance with MWCNTs hybrids Pd-cells-CNTs. It was found that the association of Pd, cells and rGO creates an interactive and synergistic environment to allow higher conductivity and catalytic activity under the same amount of carbon nanomaterial. The strategy developed in this work activates a highly reactive NPs and proposed a designable protocol for enhancing electrocatalytic activity of biocatalysts.


Assuntos
Grafite , Nanopartículas Metálicas , Shewanella , Catálise , Paládio
20.
Biosens Bioelectron ; 156: 112136, 2020 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-32174561

RESUMO

Shewanella oneidensis MR-1, a model species of exoelectrogenic bacteria (EEB), has been widely applied in bioelectrochemical systems. Biofilms of EEB grown on electrodes are essential in governing the current output and power density of bioelectrochemical systems. The MR-1 genome is exceptionally dynamic due to the existence of a large number of insertion sequence (IS) elements. However, to date, the impacts of IS elements on the biofilm-forming capacity of EEB and performance of bioelectrochemical systems remain unrevealed. Herein, we isolated a non-motile mutant (NMM) with biofilm-deficient phenotype from MR-1. We found that the insertion of an ISSod2 element into the flrA (encoding the master regulator for flagella synthesis and assembly) of MR-1 resulted in the non-motile and biofilm-deficient phenotypes in NMM cells. Notably, such a variant was readily confused with the wild-type strain because there were no obvious differences in growth rates and colonial morphologies between the two strains. However, the reduced biofilm formation on the electrodes and the deteriorated performances of bioelectrochemical systems and Cr(VI) immobilization for the strain NMM were observed. Given the wide distribution of IS elements in EEB, appropriate cultivation and preservation conditions should be adopted to reduce the likelihood that IS elements-mediated mutation occurs in EEB. These findings reveal the negative impacts of IS elements on the biofilm-forming capacity of EEB and performance of bioelectrochemical systems and suggest that great attention should be given to the actual physiological states of EEB before their applications.


Assuntos
Fontes de Energia Bioelétrica/microbiologia , Biofilmes/crescimento & desenvolvimento , Shewanella/fisiologia , Elementos de DNA Transponíveis , Eletricidade , Shewanella/genética , Shewanella/crescimento & desenvolvimento
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