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1.
Chem Asian J ; 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31478601

RESUMO

A novel approach for in situ generation AgI quantum dots by the confine of a pillar[5]arene-based supramolecular polymer network has been successfully developed. The supramolecular polymer network (SPN-QP) was constructed by bis-8-hydroxyquinoline modified pillar[5]arene derivative host (H-QP) and bis-pyridinium modified decane guest (G-PD). The SPN-QP shows ultrasensitive response for Ag+. The limits of lowest detection about is 7.44 × 10-9 M. Interestingly, when I- was added to the SPN-QP + Ag+ system, an unexpected strong warm white fluorescent emitted. After carefully investigated, we found that strong warm white fluorescence emission could be attributed to in situ formation of AgI quantum dots under the confine of the supramolecular polymer network (SPN-QP). Based on this approach, ultrasensitive detection I- was realized. The limits of lowest detection of I- is 4.40 × 10-9 M. This study provides a new way for preparation of quantum dots under the confine of supramolecular polymer network as well as ultrasensitive detection ions by in situ formation of quantum dots.

2.
J Cell Mol Med ; 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31475450

RESUMO

Myoferlin, a protein of the ferlin family, has seven C2 domains and exhibits activity in some cells, including myoblasts and endothelial cells. Recently, myoferlin was identified as a promising target and biomarker in non-small-cell lung cancer, breast cancer, pancreatic adenocarcinoma, hepatocellular carcinoma, colon cancer, melanoma, oropharyngeal squamous cell carcinoma, head and neck squamous cell carcinoma, clear cell renal cell carcinoma and endometrioid carcinoma. This evidence indicated that myoferlin was involved in the proliferation, invasion and migration of tumour cells, the mechanism of which mainly included promoting angiogenesis, vasculogenic mimicry, energy metabolism reprogramming, epithelial-mesenchymal transition and modulating exosomes. The roles of myoferlin in both normal cells and cancer cells are of great significance to provide novel and efficient methods of tumour treatment. In this review, we summarize recent studies and findings of myoferlin and suggest that myoferlin is a novel potential candidate for clinical diagnosis and targeted cancer therapy.

4.
Mol Med Rep ; 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31485657

RESUMO

Osteoarthritis (OA) is one of the most prevalent types of chronic joint diseases. Chondrocytes survival is closely associated with the destruction of joints in patients with OA. Long noncoding RNAs (lncRNAs) serve a critical role in OA. However, to the best of our knowledge, the role of lncRNAs NR024118 in OA has not been examined. In the present study, the expression levels of NR024118 in lipopolysaccharide (LPS)­induced chondrocytes was measured using reverse transcription­quantitative polymerase chain reaction (RT­qPCR) and the apoptosis levels of cells was determined using flow cytometry. The levels of scavenged reactive oxygen species and expression levels of the antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and heme oxygenase­1 (HO­1) were measured using specialized detection kits. The expression of interleukin (IL)­1ß, IL­6 and IL­18 were measured using ELISA. Expression of the cell apoptosis markers Bcl­2, Bax, Bcl­2­like protein 11, NF­κB, phosphorylated (p)­NF­κB inhibitor ß (IκBß), IκBß, p­transcription factor p65 (p65) and p65, and nuclear factor erythroid­2 related factor 2 (Nrf2) signaling pathways­associated proteins, Nrf2, HO­1 and quinone oxidoreductase­1 were detected by western blot analysis and RT­qPCR. The results indicated that in ATDC5 cells, apoptosis, oxidative stress and inflammation were significantly increased and the expression level of NR024118 was significantly decreased by LPS­mediated induction. NR024118 overexpression significantly reversed the effects of LPS treatment in the ATDC5 cell line. In addition, the overexpression of NR024118 decreased NF­κB expression levels and activated the Nrf2 signaling pathways in LPS­induced ATDC5 cells. The present study demonstrated that NR024118 attenuated the effects of LPS­induction on ATDC5 cells. These results suggest that NR024118 may be a potential target for diagnosis and treatment of OA.

5.
Open Biol ; 9(9): 190074, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31480990

RESUMO

The aim of this study was to analyse the expression pattern and elucidate the mechanistic involvement of long non-coding RNA LINC00467 in hepatocellular carcinoma (HCC). The relative expression of LINC00467 and microRNA (miR)-9-5p was determined by real-time polymerase chain reaction. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The cell proliferation was analysed by cell counting. Cell migration and invasion were monitored by Transwell assay. The luciferase reporter assay was employed to investigate the regulatory effect of miR-9-5p on LINC00467 and peroxisome proliferator-activated receptor alpha (PPARA). The endogenous PPARA protein was quantified by western blotting. It was found that LINC00467 was aberrantly decreased in HCC. The ectopic expression of LINC00467 significantly suppressed cell viability, proliferation, migration and invasion. LINC00467 functioned as a sponge for miR-9-5a and negatively regulated miR-9-5p expression. We also identified PPARA as the direct target of miR-9-5p. siRNA-mediated knockdown of PPARA in LINC00467-proficient cells promoted cell viability, migration and invasion. Our data indicate the critical involvement of LINC00467/miR-9-5p/PPARA signalling in the incidence and progression of HCC.

6.
Mol Psychiatry ; 2019 Aug 02.
Artigo em Inglês | MEDLINE | ID: mdl-31375779

RESUMO

Immune dysregulation, specifically of inflammatory processes, has been linked to behavioral symptoms of depression in both human and rodent studies. Here, we evaluated the antidepressant effects of immunization with altered peptide ligands of myelin basic protein (MBP)-MBP87-99[A91, A96], MBP87-99[A91], and MBP87-99[R91, A96]-in different models of depression and examined the mechanism by which these peptides protect against stress-induced depression. We found that a single dose of subcutaneously administered MBP87-99[A91, A96] produced antidepressant-like effects by decreasing immobility in the forced swim test and by reducing the escape latency and escape failures in the learned helplessness paradigm. Moreover, immunization with MBP87-99[A91, A96] prevented and reversed depressive-like and anxiety-like behaviors that were induced by chronic unpredictable stress (CUS). However, MBP87-99[R91, A96] tended to aggravate CUS-induced anxiety-like behavior. Chronic stress increased the production of peripheral and central proinflammatory cytokines and induced the activation of microglia in the prelimbic cortex (PrL), which was blocked by MBP87-99[A91, A96]. Immunization with MBP-derived altered peptide ligands also rescued chronic stress-induced deficits in p11, phosphorylated cyclic adenosine monophosphate response element binding protein, and brain-derived neurotrophic factor expression. Moreover, microinjections of recombinant proinflammatory cytokines and the knockdown of p11 in the PrL blunted the antidepressant-like behavioral response to MBP87-99[A91, A96]. Altogether, these findings indicate that immunization with altered MBP peptide produces prolonged antidepressant-like effects in rats, and the behavioral response is mediated by inflammatory factors (particularly interleukin-6), and p11 signaling in the PrL. Immune-neural interactions may impact central nervous system function and alter an individual's response to stress.

8.
Int J Low Extrem Wounds ; : 1534734619870098, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31464167

RESUMO

Venous leg ulcers are a manifestation of lower extremity chronic venous disorder. Venous hypertension caused by abnormal venous blood flow is considered to be the primary mechanism of venous ulcers. The etiology of lower extremity venous ulcer is complicated, and it is difficult to be treated. At present, it has achieved a certain effect for venous leg ulcers through the combination of drugs, pressure therapy, and surgical treatment. Budd-Chiari syndrome is a rare syndrome characterized by portal hypertension and/or inferior vena cava syndrome. Treatment of Budd-Chiari syndrome includes anticoagulation, thrombolysis, angioplasty, stenting, transjugular intrahepatic portosystemic shunt, and liver transplantation. This article reports an elderly female patient with recurrent ulceration of both lower extremities that healed poorly after long-term dressing and skin grafting. During further examination, she was found to have Budd-Chiari syndrome. Through multidisciplinary treatment, which includes removing the inferior vena cava stenosis and implanting the ulcer wound, the ulcer wounds then healed.

10.
Medicine (Baltimore) ; 98(35): e16972, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31464942

RESUMO

Single nuclear polymorphism (SNP) of programmed cell death 1 (PD-1) was reported associated with hepatitis B virus (HBV) infection, but the SNP sites studied were limited. Whether the combination of 2 or more SNP sites could better represent the relationship between PD-1 SNP and HBV infection was not studied.Eight hundred ninety-eight HBV-infected patients (222 asymptomatic carriers [AsC], 276 chronic hepatitis B, 105 acute-on-chronic liver failure, and 295 liver cirrhosis) and 364 health controls of South China were enrolled in this study. Four PD-1 SNPs (rs10204525, rs2227982, rs41386349, and rs36084323) were selected and detected by TaqMan probe. The frequency of allele, genotype, and combination of different SNPs were compared between different groups.For allele frequency analysis, G allele of rs10204525 was protective factor (odds ratio (OR) = 0.823, 95% confidence interval (CI) = 0.679-0.997, P = .046) and T allele of rs2227982 was predisposing factor (OR = 1.231, 95% CI = 1.036-1.463, P = .018) in HBV infection. When analyzed in genotype frequency, the genotype GG of rs10204525 and CC of rs2227982 were protective factor of HBV infection. Combination of rs10204525 GG and rs2227982 CC was potent protective factor of HBV infection (OR = 0.552, 95% CI = 0.356-0.857, P = .007) and was also associated with lower HBV load (OR = 0.201, 95% CI = 0.056-0.728, P = .008) in AsC. The 4 SNP sites were not associated with progression of HBV-related liver disease.Rs10204525 and rs2227982 of PD-1 associate with HBV infection and combination of the 2 SNP sites can better predict host susceptibility in HBV infection.

11.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(7): 790-794, 2019 Jul 28.
Artigo em Chinês | MEDLINE | ID: mdl-31413217

RESUMO

OBJECTIVE: To explore the effect of application of soft tissue loosening and acetabular reconstruction in hip replacement for patients with severe femoral head necrosis on joint function.
 Methods: From June 2012 to August 2016, 68 patients with severe femoral head necrosis (Ficat III, IV) underwent total hip replacement with soft tissue release and acetabular reconstruction at the Second Xiangya Hospital of Central South University. Total hip replacement is performed by the posterolateral approach. The acetabulum was rebuilt and the length of the affected limb was prolonged after clearing the scar tissue, proliferating the epiphysis, releasing the abductor muscle group and the adductor muscle group, dissecting the soft tissue around the acetabulum. One year after surgery, Harris score, X-ray positive lateral radiograph for the affected side and full-length X-ray examination for both lower extremities were performed to evaluate the curative effect.
 Results: The postoperative follow-up time ranged from 1.0 to 5.5 years. All patients' femoral heads returned to normal anatomical position and the affected limb length was restored to 1.5-3.5 cm; all patients did not damage the sciatic nerve. The Harris scores for 68 patients increased from 38.6±7.5 to 78.2±5.7 (P=0.029) in the first year after surgery.
 Conclusion: During hip replacement surgery for severe femoral head necrosis, soft tissue dissection and acetabular reconstruction can be used to ensure anatomical reconstruction for the acetabular fossa and to improve abductor function.


Assuntos
Artroplastia de Quadril , Necrose da Cabeça do Fêmur , Acetábulo , Cabeça do Fêmur , Necrose da Cabeça do Fêmur/cirurgia , Seguimentos , Humanos , Radiografia , Resultado do Tratamento
12.
Stud Health Technol Inform ; 264: 378-382, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31437949

RESUMO

An attractive feature of non-lattice-based ontology auditing methods is its ability to not only identify potential quality issues, but also automatically generate the corresponding fixes. However, exhaustive manual evaluation of the validity of suggested changes remains a challenge shared with virtually all auditing methods. To address this challenge, we explore machine learning techniques as an aid to systematically evaluate the strength of auto-suggested relational changes in the context of existing knowledge embedded in an ontology. We introduce a hybrid convolutional neural network and multilayer perception (CNN-MLP) classifier using a combination of graph, concept features and concept embeddings. We use lattice subgraphs to generate a curated, loosely-coupled training set of positive and negative instances to train the classifier. Our result shows that machine learning techniques have the potential to alleviate the manual effort required for validating and confirming changes generated by non-lattice-based auditing methods for SNOMED CT.

13.
Stud Health Technol Inform ; 264: 487-491, 2019 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-31437971

RESUMO

According to the latest statistics of the China National Health Protection Commission, the prevalence of adult diabetics in China has reached 11.6%, and the number of patients has exceeded 114 million. Understanding the needs of diabetics and what kind of problems they are anxious about are crucial for doctors, hospitals and other health care providers, which can be used to ameliorate patient education services and help patients to improve their disease management skills. Hence we have conducted a study to analyze the questions about diabetes collected from a Chinese health website; the number of which is 151,589. We have divided these questions into 9 categories using a convolutional neural network. The shocking results showed that the questions about prevention only account for 1.23%. And a Chinese patented drug Xiaoke pill, the main component of which is glibenclamide, ranks fourth among the drugs the user cares most about due to the cheap price. However, patients know very little about its side effects.

14.
Biofabrication ; 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31470437

RESUMO

The ability to fabricate perfusable, small-diameter vasculature is a foundational step toward generating human tissues/organs for clinical applications. Currently, it is highly challenging to generate vasculature integrated with smooth muscle and endothelium that replicates the complexity and functionality of natural vessels. Here, a novel method for directly printing self-standing, small-diameter vasculature with smooth muscle and endothelium is presented through combining tailored mussel-inspired bioink and unique "fugitive-migration" tactics, and its effectiveness and advantages over other methods (i.e., traditional alginate/calcium hydrogel, post-perfusion of endothelial cells) are demonstrated. The biologically inspired, catechol-functionalized, gelatin methacrylate (GelMA/C) undergoes rapid oxidative crosslinking in situ to form an elastic hydrogel, which can be engineered with controllable mechanical strength, high cell/tissue adhesion, and excellent bio-functionalization. The results demonstrate the bioprinted vascular construct possessed numerous favorable, biomimetic characteristics such as proper biomechanics, higher tissue affinity, vascularized tissue manufacturing ability, beneficial perfusability and permeability, excellent vasculoactivity, and in vivo autonomous connection (~2 weeks) as well as vascular remodeling (~6 weeks). The advanced achievements in creating biomimetic, functional vasculature illustrate significant potential toward fabricating small-diameter vasculature and eventually generating a complicated vascularized tissue/organ for clinic translation.

15.
Anal Chem ; 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31423770

RESUMO

Sulfur dioxide (SO2) plays significant roles in regulating cell apotosis and inflammation. However, there are complex interactions between small biomolecules in cells, and the identification of these coexisting biomarkers remains a challenge. Herein, we report an AND logic gate based fluorescent probe (NY-Lyso), operating by responding to pH differences between organelles in cell and selectively reacting with bisulfite (HSO3-). This approach allows the fluorescence of the probe to remain silent under neutral or alkaline conditions, notably, is activated by costimulation of lower pH and bisulfite. Furthermore, it was confirmed to be biocompatible and could be employed to monitor HSO3- in lysosomes of living cells. The proposed method demonstrated more practical and outstanding capabilities in targeted and real-time monitoring, providing an effective optical tool for biomarker sensing.

17.
Radiat Res ; 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31390312

RESUMO

Radiation-induced acute skin injury and consequent fibrosis are common complications of cancer radiotherapy and radiation accidents. Stromal cell-derived factor-1α (SDF-1α) and its receptor, CXC chemokine receptor 4 (CXCR4) have been shown to be involved in multiple cellular events. However, the role of SDF-1α/CXCR4 axis in radiation-induced acute injury and fibrosis of skin has not been reported. In this study, we found that the expression of SDF-1α and CXCR4 was significantly increased in irradiated skin tissues of humans, monkeys and rats, compared to their nonirradiated counterparts. Mice with keratinocyte-specific ablation of CXCR4 showed less severe skin damage than wild-type mice after receiving a 35 Gy dose of radiation. Consistently, subcutaneous injection of AMD3100, an FDA approved SDF-1α/CXCR4 inhibitor, attenuated skin injury and fibrosis induced by exposure to radiation in a rat model. Mechanically, the SDF-1α/CXCR4 axis promotes pro-fibrotic TGF-ß/Smad signaling through the PI3K-MAPK signaling cascade in human keratinocyte HaCaT cells and skin fibroblast WS1 cells. AMD3100 inhibited Smad2 nuclear translocation and transcriptional activity of Smad2/3 induced by radiation, which suppressed the pro-fibrotic TGF-ß/Smad signaling pathway activated by exposure. Taken together, these findings demonstrate the involvement of SDF-1α/CXCR4 axis in radiation-induced acute injury and fibrosis of skin, and indicate that AMD3100 would be an effective countermeasure against these diseases.

18.
J Clin Ultrasound ; 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31392732

RESUMO

Primary cardiac tumors are rare, but papillary fibroelastoma (PFE) is reportedly the most common form, which usually occurs on the left-side valves of the heart. However, PFE involving the tricuspid and pulmonary valves has also been documented. Although PFE is benign and seldom associated with valvular dysfunction, the associated embolic complications may lead to serious consequences. Most patients with PFE lack specific clinical symptoms and the diagnosis is incidental. Surgical resection is the mainstay treatment for PFE in order to prevent the occurrence of embolic complications. In this report, we present a case of a rare asymptomatic PFE of the pulmonary valve, which was incidentally noted during a routine examination with transthoracic echocardiography (TEE). There was neither valvular dysfunction nor hemodynamic change. The PFE was surgically removed, and the diagnosis was further confirmed with histopathology.

19.
QJM ; 2019 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-31393580

RESUMO

The adenoid cystic carcinoma (ACC) is the frequent pathology occurring in the salivary glands. It is usually slow growing, but invasive, and thereby spread via perineural invasion, with haematogenous and lymphatogenous metastasis. In this article, we present a rare asymptomatic case of an ACC of salivary gland with coincident mandible invasion and multiple pulmonary metastasis (PM). Immunohistochemically, a series of molecular markers were used to determine which a biologically subtype exists, and to provide histological and tumor microenvironmental data about PM of ACC. Clinically, it is also valuable in screening PM and in selecting case to identify the primary tumor.

20.
Front Immunol ; 10: 1620, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31396207

RESUMO

In rheumatoid arthritis(RA) pathogenesis, activated RA fibroblast-like synoviocytes (RA-FLSs) exhibit similar proliferative features as tumor cells and subsequent erosion to cartilage will eventually lead to joint destruction. Therefore, it is imperative to search for compounds, which can effectively inhibit the abnormal activation of RA-FLSs, and retard RA progression.3'3-Diindolylmethane (DIM), the major product of the acid-catalyzed oligomerization of indole-3-carbinol from cruciferous vegetables, has been reported to be functionally relevant to inhibition of migration, invasion and carcinogenesis in some solid tumors. In this study, we explored the anti-proliferation, anti-metastasis and anti-inflammation effects of DIM on RA-FLSs as well as the underlying molecular mechanisms. To do this, primary RA-FLSs were isolated from RA patients and an animal model. Cell proliferation, migration and invasion were measured using CCK-8, scratch, and Transwell assays, respectively. The effects of DIM on Matrix metalloproteinases (MMPs) and some inflammatory factors mRNA and key molecules such as some inflammatory factors and those involved in aberrantly-activated signaling pathway in response to tumor necrosis factor α(TNF-α), a typical characteristic mediator in RA-FLS, were quantitatively measured by real-time PCR and western blotting. Moreover, the effect of DIM on adjuvant induced arthritis(AIA) models was evaluated with C57BL/6 mice in vivo. The results showed that DIM inhibited proliferation, migration and invasion of RA-FLS in vitro. Meanwhile, DIM dramatically suppressed TNF-α-induced increases in the mRNA levels of MMP-2, MMP-3, MMP-8, and MMP-9; as well as the proinflammatory factors IL-6, IL-8, and IL-1ß. Mechanistic studies revealed that DIM is able to suppress phosphorylated activation not only of p38, JNK in MAPK pathway but of AKT, mTOR and downstream molecules in the AKT/mTOR pathway. Moreover, DIM treatment decreased expression levels of proinflammatory cytokines in the serum and alleviated arthritis severity in the knee joints of AIA mice. Taken together, our findings demonstrate that DIM could inhibit proliferation, migration and invasion of RA-FLSs and reduce proinflammatory factors induced by TNF-α in vitro by blocking MAPK and AKT/mTOR pathway and prevent inflammation and knee joint destruction in vivo, which suggests that DIM might have therapeutic potential for RA.

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