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1.
Curr Microbiol ; 2020 Jan 13.
Artigo em Inglês | MEDLINE | ID: mdl-31932997

RESUMO

Fusarium oxysporum f. sp. radicis-lycopersici (Forl) causes Fusarium crown and root rot of tomato, leading to severe yield losses. Chinese chive and the Chinese chive extract reportedly have antifungal effects. In this study, Chinese chive extract treatments inhibited Forl spore germination, with an EC50 of 0.40 g ml-1 in vitro. Furthermore, the mechanism underlying the fungicidal effects of the Chinese chive extract was analyzed by RNA sequencing. A total of 1252 differentially expressed genes (DEGs) were detected, of which 396 were upregulated and 856 were downregulated. The DEGs were related to starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, galactose metabolism, fatty acid metabolism, sphingolipid metabolism, glycerophospholipid metabolism, peroxisomes, ribosome biogenesis in eukaryotes, mismatch repair, and the phosphatidylinositol signaling system, implying these pathways contribute to the fungicidal activity of the Chinese chive extract. The qRT-PCR results verified the accuracy of the RNA sequencing data. Thus, the Chinese chive extract can inhibit Forl spore germination by affecting spore nutrient metabolism.

2.
Sci Rep ; 9(1): 20240, 2019 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-31882801

RESUMO

Tomato is an important vegetable crop grown worldwide. Grafting is an agricultural technique that is used to improve growth, yield, and resistance to diverse stresses in tomato production. Here, we examined the differences between the scion of heterografted ('Provence'/'Haomei') and homografted ('Provence'/'Provence') tomato seedlings. We observed anatomical changes during the graft-union healing process in heterografted and homografted tomato seedlings and conducted transcriptome analyses of the 'Provence' scion from both graft combinations. With the development of calli from both graft partners, the isolation layer became thinner at 16 d after grafting (DAG). Compared with that of homografts, the healing in heterografts was slightly delayed, but the graft union had completely healed at 21 DAG. In total, 858 significantly differentially expressed genes were detected between the transcriptomes of heterografts and homografts at 16 DAG. Functional pathways identified by GO and KEGG enrichment analyses were associated with primary and secondary metabolism, hormone signalling, transcription factor regulation, transport, and responses to stimuli. Many differentially expressed genes were involved in pathways associated with mitogen-activated protein kinase signalling, plant hormone signalling, and oxidative stress. A number of transcription factors were up-regulated in the scion of heterografted seedlings. The results provide a valuable resource for the elucidation of the molecular mechanisms, and candidate genes for functional analyses, of heterograft and homograft systems.

3.
Biomaterials ; 224: 119488, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31562997

RESUMO

Improved strategies for the treatment of tendon defects are required to successfully restore mechanical function and strength to the damaged tissue. This remains a scientific and clinical challenge, given the tendon's limited innate regenerative capacity. Here, we present an engineering solution that stimulates the host cell's remodeling abilities. We combined precision-designed templates with subcutaneous implantation to generate decellularized autologous extracellular matrix (aECM) scaffolds that had highly aligned microchannels after removal of templates and cellular components. The aECM scaffolds promoted rapid cell infiltration, favorable macrophage responses, collagen-rich extracellular matrix (ECM) synthesis, and physiological tissue remodeling in rat Achilles tendon defects. At three months post-surgery, the mechanical strength of tenocyte-populated 'neo-tendons' was comparable to pre-injury state tendons. Overall, we demonstrated an in vivo bioengineering strategy for improved restoration of tendon tissue, which also offers wider implications for the regeneration of other highly organized tissues.

4.
Bioresour Technol ; 285: 121313, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30959388

RESUMO

A constructed wetland (CW) was established to explore the influence of carbon addition (glucose or sodium acetate) on nitrogen removal and greenhouse gas (GHG) emissions at chemical oxygen demand to nitrogen ratios (COD/Ns) of 0, 4, 7. Results showed that the type of carbon source and COD/N significantly influenced the CW performance, in which the electrons transfer determined the regulation of denitrification, methanogenesis and respiration. Higher N2O emissions were consistent with higher nitrite accumulation at low COD/N because of electrons competition. The residual carbon source after near-complete denitrification could be further utilized by methanogenesis. Sodium acetate was superior to glucose in promoting denitrification and reducing global warming potential (GWP). In addition, bacteria sequencing and functional genes confirmed the important role of the type of carbon source on controlling nitrogen removal, carbon consumption and GHG emissions in microbial communities.


Assuntos
Gases de Efeito Estufa , Áreas Alagadas , Carbono , Desnitrificação , Elétrons , Nitrogênio
5.
BMC Pediatr ; 19(1): 133, 2019 04 25.
Artigo em Inglês | MEDLINE | ID: mdl-31023291

RESUMO

BACKGROUND: Neonatal hypoglycemia is tightly related to adverse neurodevelopmental and brain injury outcomes. METHODS: A total of 195 infants who were born from diabetic mothers with a low blood glucose level (< 2.6 mM) within 0.5 h after birth were enrolled in this prospective cohort study. Of these, 157 infants who had neonatal hypoglycemia (group A) were followed up, and this group was further divided into A1 [blood glucose concentration (BGC) < 2.6 mM at < 2 h after birth], A2 (BGC < 2.6 mM at 2-24 h after birth), and A3 (BGC < 2.6 mM at > 24 h after birth). A total of 144 infants whose mothers had no high risk for gestational diabetes mellitus were followed up as the control group during the same period. The neurodevelopment of the infants was evaluated by the Gesell scoring method. RESULTS: The adaptability in the A2 and A3 subgroups was significantly lower than that in the control group (73.9 ± 6.6 vs. 87.9 ± 11.2; 71.5 ± 8.9 vs. 87.9 ± 11.2, respectively). There were significantly more mothers who used insulin during the perinatal period in A3 than in A1 and A2 (31% vs. 2%; 31% vs. 7.9%, respectively). The mothers of babies in subgroups A2 and A3 gained more weight than those of the control group (15.3 ± 1.9 kg vs. 11.1 ± 2.2 kg; 14.8 ± 2.6 kg vs. 11.1 ± 2.2 kg, respectively). CONCLUSIONS: Long and repeated neonatal hypoglycemia caused poor adaptability. The babies of mothers who used insulin or had a high weight gain during pregnancy were associated with severe or persistent neonatal hypoglycemia.

6.
Food Chem ; 275: 497-503, 2019 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-30724225

RESUMO

This study develops a reliable radial basis function neural networks (RBFNNs) to estimate freshness for tilapia fillets stored under non-isothermal conditions by using optimal wavelengths from hyperspectral imaging (HSI). The results show that, for tilapia fillet stored at -3, 0, 4, 10, and 15 °C and non-isothermal conditions, total volatile basic nitrogen (TVB-N), total aerobic counts (TAC), and the K value increase whereas sensory scores decrease with increasing storage time. To simplify the models, nine optimal wavelengths were selected by using the successive projections algorithm (SPA), following which SPA-RBFNN models were built based on the selected wavelengths and the values of TVB-N, TAC, K, and sensory evaluations for tilapia fillets store isothermally. The ability of the models based on HSI to predict the freshness indicators were verified for tilapia fillets stored under non-isothermal conditions. HSI thus has an excellent potential for nondestructive determination of freshness in tilapia fillets.


Assuntos
Qualidade dos Alimentos , Armazenamento de Alimentos/métodos , Alimentos Marinhos , Temperatura Ambiente , Tilápia , Animais , Nitrogênio/análise , Alimentos Marinhos/análise , Alimentos Marinhos/microbiologia , Tilápia/microbiologia
7.
Bioresour Technol ; 271: 298-305, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30290322

RESUMO

Simultaneous nitrification, anammox and denitrification (SNAD) process was developed in subsurface flow constructed wetlands (SFCWs) to treat polluted surface water. The effects of vegetation, hydraulic retention time (HRT), C/N, and influent nitrogen forms on nitrogen removal and microbial communities were investigated. Results showed that denitrification- and anammox-dominant SNAD corresponded to nitrate- and ammonia-dominant influent, respectively, and both could achieve more efficient nitrogen removal in planted SFCWs than the unplated. These higher efficiencies were due to the microbial growth, organic carbon release, oxygen supply and plant uptake promoted by vegetation. The electron donors accelerated denitrification but inhibited ammonia oxidation with deficient oxygen. Anammox contributed to nitrogen removal of 27.34% under oxygen-limited conditions without vegetation. Anammox combined with denitrification and plant uptake were over 90% in planted SFCWs. For the investigated factors, the ammonia, nitrate and C/N were the most significant ones influencing the microbial communities, further nitrogen removal pathways and performances.


Assuntos
Áreas Alagadas , Amônia/química , Desnitrificação , Nitrificação , Nitrogênio/química , Oxirredução , Oxigênio/química
8.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(12): 1506-1511, 2019 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-32029038

RESUMO

OBJECTIVE: To evaluate the incidence and mortality risk factors of pregnancy-related acute kidney injury (PR-AKI) in intensive care unit (ICU). METHODS: A retrospective analysis was conducted. Critically ill pregnancies admitted to ICU of Shandong University Affiliated Provincial Hospital from January 1st, 2012 to December 31st, 2016 were enrolled. Based on the Kidney Disease: Improving Global Outcomes (KDIGO)-acute kidney injury (AKI) criteria, patients were divided into two groups: PR-AKI group and non-PR-AKI group. Clinical characteristics and laboratory data of two groups were compared. Risk factors of incidence and mortality of PR-AKI patients were analyzed, and the receiver operating characteristic (ROC) curve was drawn to evaluate the value of these risk factors in predicting mortality of PR-AKI patients in ICU. RESULTS: (1) A total of 219 pregnancies in ICU were included in the analysis, 85 cases (38.8%) were diagnosed with PR-AKI, with 29.4% in AKI stage 1, 27.1% in AKI stage 2 and 43.5% in AKI stage 3. (2) Nineteen of 219 critically ill pregnancies died in ICU, the total ICU mortality was 8.7%. The mortality of PR-AKI group was higher than non-PR-AKI group (16.5% vs. 3.7%, P = 0.003). The mortality was worsened with increasing severity of AKI (4.0% for AKI stage 1, 4.3% for AKI stage 2, 32.4% for AKI stage 3). (3) Acute fatty liver of pregnancy (AFLP) and lactate (Lac) were the independent risk factors for PR-AKI [AFLP: odds ratio (OR) = 6.081, 95% confidence interval (95%CI) was 1.587-23.308, P = 0.008; Lac: OR = 1.460, 95%CI was 1.078-1.977, P = 0.014]. (4) Age, Lac, acute physiology and chronic health evaluation II (APACHE II) and sequential organ failure assessment (SOFA) were the independent risk factors associated with the mortality of PR-AKI patients in ICU (age: OR = 1.130, 95%CI was 1.022-1.249, P = 0.017; Lac: OR = 1.198, 95%CI was 1.009-2.421, P = 0.039; APACHE II: OR = 1.211, 95%CI was 1.102-1.330, P < 0.001; SOFA: OR = 1.411, 95%CI was 1.193-1.669, P < 0.001). (5) ROC curve analysis showed that age, Lac, APACHE II score and SOFA score all had good predictive values for in-hospital mortality among PR-AKI patients in ICU, the cut-off value was 29 years old, 3.8 mmol/L, 16 and 8, respectively, and the AUC was 0.751, 0.757, 0.892 and 0.919, respectively (all P < 0.01). CONCLUSIONS: The incidence and mortality of PR-AKI of critically ill pregnancies in ICU are high. Increased age, Lac, APACHE II score and SOFA score are independent risk factors associated with the mortality of PR-AKI patients in ICU, and have good predictive values for prognosis.


Assuntos
Lesão Renal Aguda/epidemiologia , Estado Terminal/epidemiologia , Adulto , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Feminino , Humanos , Incidência , Unidades de Terapia Intensiva , Gravidez , Prognóstico , Curva ROC , Estudos Retrospectivos , Fatores de Risco
9.
Clin Lab ; 64(5): 877-882, 2018 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-29739050

RESUMO

BACKGROUND: Urine is a good alternative body fluid for gonadotropin studies. There was limited information about the effects of different storage conditions on urinary gonadotropin measurement by using immunochemiluminometric assay (ICMA). METHODS: ICMA was used to determine gonadotropin in urine stored under different conditions, such as different pH, storage time, and cycles of freeze-thaw. RESULTS: Luteinizing hormone (LH) level was not significantly affected at pH 2.5 to 10.5 or being stored at 4°C for 3 days. Follicular stimulating hormone (FSH) level was not significantly changed at pH 3.5 to 10.5 or throughout 49-day storage at 4°C in the absence of glycerol. LH was significantly decreased after freeze-thawing twice, while FSH was resistant to freeze-thaw procedures. CONCLUSIONS: LH and FSH can be determined by ICMA in normal urine pH range (4.6 ~ 8.0). Urine LH is more sensitive to long-term storage and multiple freeze-thaw procedures than FSH.


Assuntos
Criopreservação/métodos , Hormônio Foliculoestimulante/urina , Imunoensaio/métodos , Hormônio Luteinizante/urina , Adulto , Temperatura Baixa , Congelamento , Humanos , Concentração de Íons de Hidrogênio , Masculino , Reprodutibilidade dos Testes , Fatores de Tempo
10.
Biosci Rep ; 38(3)2018 05 29.
Artigo em Inglês | MEDLINE | ID: mdl-29559566

RESUMO

Many long non-coding RNAs (lncRNAs), including lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), are involved in various cardiac diseases. We evaluated the effects of tag single nucleotide polymorphisms (tag-SNPs) on MALAT1 gene in a Chinese population of children with congenital heart disease (CHD). In the present study, 713 CHD patients and 730 gender- and age-matched children without CHD were genotyped for MALAT1 tag-SNPs rs11227209, rs619586, and rs3200401. Further investigation of SNP's function was performed by luciferase assay. Statistical analyses, including uni- and multivariate logistic regression were performed to quantitate the association between these tag SNPs and CHD. We discovered that MALAT1 rs619586 GG allele was significantly associated with lower risk of CHD (odds ratio (OR) = 0.77, 95% confidence interval (CI) = 0.59-0.92, P=0.014) in additive model. Functional investigation indicated that G allele of rs619586 could trigger higher expression of MALAT1. We demonstrated that the functional MALAT1 polymorphism rs619586 A>G was significantly associated with CHD susceptibility in Chinese population, potentially via regulating MALAT1 expression.


Assuntos
Predisposição Genética para Doença , Cardiopatias Congênitas/genética , RNA Longo não Codificante/genética , Idade de Início , Pré-Escolar , China , Feminino , Regulação da Expressão Gênica/genética , Estudos de Associação Genética , Cardiopatias Congênitas/fisiopatologia , Humanos , Lactente , Masculino , Polimorfismo de Nucleotídeo Único/genética , Fatores de Risco
11.
Theor Appl Genet ; 131(1): 1-12, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-29116330

RESUMO

KEY MESSAGE: Using map-based cloning of ts gene, we identified a new sort of gene involved in the initiation of multicellular tender spine in cucumber. The cucumber (Cucumis sativus L.) fruit contains spines on the surface, which is an extremely valuable quality trait affecting the selection of customers. In this study, we elaborated cucumber line NC072 with wild type (WT) hard fruit spines and its spontaneous mutant NC073, possessing tender and soft spines on fruits. The mutant trait was named as tender spines (ts), which is controlled by a single recessive nuclear gene. We identified the gene ts by map-based cloning with an F2 segregating population of 721 individuals generated from NC073 and WT line SA419-2. It was located between two markers Indel6239679 and Indel6349344, 109.7 kb physical distance on chromosome 1 containing fifteen putative genes. With sequencing and quantitative reverse transcription-polymerase chain reaction analysis, the Csa1G056960 gene was considered as the most possible candidate gene of ts. In the mutant, Csa1G056960 has a nucleotide change in the 5' splicing site of the second intron, which causes different splicing to delete the second exon, resulting in a N-terminal deletion in the predicted amino acid sequence. The gene encodes a C-type lectin receptor-like tyrosine-protein kinase which would play an important role in the formation of cucumber fruit. This is firstly reported of a receptor kinase gene regulating the development of multicellular spines/trichomes in plants. The ts allele could accelerate the molecular breeding of cucumber soft spines.


Assuntos
Cucumis sativus/genética , Genes de Plantas , Tricomas/genética , Mapeamento Cromossômico , DNA de Plantas/genética , Frutas/genética , Genes Recessivos , Fenótipo , Sítios de Splice de RNA , Tricomas/crescimento & desenvolvimento
12.
Cancer Res ; 77(18): 4823-4834, 2017 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-28729416

RESUMO

Sphingosine kinase 1 (SK1) is a key regulator of the cellular balance between proapoptotic and prosurvival sphingolipids. Oncogenic signaling by SK1 relies on its localization to the plasma membrane, which is mediated by the calcium and integrin binding protein CIB1 via its Ca2+-myristoyl switch function. Here we show that another member of the CIB family, CIB2, plays a surprisingly opposite role to CIB1 in the regulation of SK1 signaling. CIB2 bound SK1 on the same site as CIB1, yet it lacks the Ca2+-myristoyl switch function. As a result, CIB2 blocked translocation of SK1 to the plasma membrane and inhibited its subsequent signaling, which included sensitization to TNFα-induced apoptosis and inhibition of Ras-induced neoplastic transformation. CIB2 was significantly downregulated in ovarian cancer and low CIB2 expression was associated with poor prognosis in ovarian cancer patients. Notably, reintroduction of CIB2 in ovarian cancer cells blocked plasma membrane localization of endogenous SK1, reduced in vitro neoplastic growth and tumor growth in mice, and suppressed cell motility and invasiveness both in vitro and in vivo Consistent with the in vitro synergistic effects between the SK1-specific inhibitor SK1-I and standard chemotherapeutics, expression of CIB2 also sensitized ovarian cancer cells to carboplatin. Together, these findings identify CIB2 as a novel endogenous suppressor of SK1 signaling and potential prognostic marker and demonstrate the therapeutic potential of SK1 in this gynecologic malignancy. Cancer Res; 77(18); 4823-34. ©2017 AACR.


Assuntos
Biomarcadores Tumorais/metabolismo , Proteínas de Ligação ao Cálcio/metabolismo , Regulação Neoplásica da Expressão Gênica , Neoplasias Ovarianas/patologia , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Animais , Apoptose , Biomarcadores Tumorais/genética , Proteínas de Ligação ao Cálcio/genética , Movimento Celular , Proliferação de Células , Feminino , Humanos , Camundongos , Camundongos Endogâmicos NOD , Camundongos SCID , Invasividade Neoplásica , Estadiamento de Neoplasias , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/metabolismo , Fosfotransferases (Aceptor do Grupo Álcool)/genética , Prognóstico , Células Tumorais Cultivadas , Ensaios Antitumorais Modelo de Xenoenxerto
13.
Medicine (Baltimore) ; 96(9): e5795, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28248850

RESUMO

BACKGROUD: The aim of the study was to evaluate the effect of early parenteral iron supplementation combined erythropoietin for prevention of anemia in preterm infants. METHODS: In total, 96 preterm infants were randomly assigned to 3 groups: a control group receiving standard parenteral nutrition (group 1: n = 31), an iron-supplemented group (group 2: IS, n = 33), and an iron-supplemented combined erythropoietin group (group 3: IS+EPO, n = 32). The primary objective was to assess hemoglobin (Hb) levels. The secondary objectives included assessment of red blood cell counts (RBC), mean cell volume (MCV), serum iron, ferritin, percentages of reticulocyte (RET), total iron binding capacity (TIBC) and oxidative stress, which was assessed by measuring plasma levels of malondialdehyde and superoxide dismutase at baseline and at 2 weeks. The blood routine indices including Hb, RBC, MCV, and percentages of RET were measured at corrected age of 1 and 3 months. RESULTS: At 2 weeks of life, the percentages of reticulocyte in group 2 and group 3 were significantly higher than those in group 1 (2.1±0.4, 2.5±0.3, and 1.7±0.3, respectively, P < 0.001, P<0.001), whereas TIBC were significantly lower than those in group 1 (36.7±4.6, 36.0±4.7, and 41.6 ±â€Š5.2 respectively, P = 0.011, P = 0.006). There were no significant differences in RBC counts, the levels of hemoglobin, ferritin, malondialdehyde, and superoxide dismutase among the 3 groups at 2weeks of life. RBC, Hb, MCV, body weight, body length, and head circumference at a corrected age of 1 month did not differ among 3 groups. At corrected age of 3months, more infants in the control group had abnormal Hb and MCV levels (Hb levels: 114.3 ±â€Š21.3, 123.7 ±â€Š31.6, and 125.1 ±â€Š21.2, P = 0.021, P = 0.034, respectively; MCV: 74.1 ±â€Š3.5, 78.3 ±â€Š4.7 and 79.1 ±â€Š5.2, P = 0.017, P = 0.012, respectively), whereas cases of oral iron, cases of breastfeeding, RBC, body weight, body length, and head circumference were not different among 3 groups. CONCLUSION: Early parenteral iron supplementation combined erythropoietin in preterm infants improved the percentages of reticulocyte, decreased total iron binding capacity, and improved the Hb and MCV levels at 3 months of age. Early parenteral iron supplementations with EPO were beneficial for the preterm infants.


Assuntos
Anemia/tratamento farmacológico , Eritropoetina/administração & dosagem , Ferro/administração & dosagem , Feminino , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Masculino
14.
Bioresour Technol ; 234: 243-252, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28324826

RESUMO

Three novel floating treatment wetlands, including autotrophic enhanced floating treatment wetland (AEFTW), heterotrophic enhanced floating treatment wetland (HEFTW) and enhanced floating treatment wetland (EFTW) were developed to remove nitrogen from the secondary effluent. Results showed that the analogously excellent nitrogen removal performance was achieved in AEFTW and HEFTW. About 89.4% of the total nitrogen (TN) was removed from AEFTW at a low S/N of 0.9 and 88.5% from HEFTW at a low C/N of 3.5 when the hydraulic retention time (HRT) was 1d in summer. Higher nitrification and denitrification performance were achieved in AEFTW. Addition of electron donors effectively reduced the N2O emission, especially in summer and autumn. High-throughput sequencing analysis revealed that the electron donors distinctly induced the microbial shifts. Dechloromonas, Thiobacillus and Nitrospira became the most predominant genus in HEFTW, AEFTW and EFTW. And autotrophic and heterotrophic denitrification could simultaneously occur in HEFTW and AEFTW.


Assuntos
Nitrogênio , Áreas Alagadas , Processos Autotróficos , Desnitrificação , Purificação da Água
15.
J Matern Fetal Neonatal Med ; 30(1): 13-16, 2017 01.
Artigo em Inglês | MEDLINE | ID: mdl-26956782

RESUMO

BACKGROUND: This study aimed to evaluate the effect of early probiotic administration on gut microflora and influence on feeding in pre-term infants. METHODS: A double-blind, randomized, controlled clinical study was conducted to assess the effect of probiotics [live, combined lactobacillus and bifidobacterium (LCB)] supplementation in pre-term infants. Sixty hospitalized pre-term babies were randomly assigned to two groups: a probiotics-supplemented group and the control group. The primary endpoint was measurement of lactobacillus and bifidobacterium in the gut. The secondary outcome was the rate of feeding intolerance. RESULTS: In the first weekend, the quantity of gut lactobacillus and bifidobacterium was significantly higher in the probiotics-supplemented group than in the control group [7.84 ± 0.35 versus 6.39 ± 0.53 (log copy number/g wet fecal weight), p = 0.013; 8.52 ± 0.23 versus 7.01 ± 0.48, p = 0.024, respectively]. In the second weekend, the amount of gut lactobacillus and bifidobacterium in the probiotics-supplemented group remained significantly higher (8.62 ± 0.28 versus 7.34 ± 0.59, p = 0.036 and 9.45 ± 0.64 versus 7.85 ± 0.43, p = 0.007, respectively). Fewer patients in the probiotics-supplemented group developed a feeding intolerance (13.3% versus 46.7%, p = 0.013). CONCLUSIONS: Probiotic supplementation in the hospitalized pre-term infants in the first 2 weeks of life resulted in higher amounts of lactobacillus and bifidobacterium in the gut and a concomitant lower rate of feeding intolerance.


Assuntos
Bifidobacterium , Enterococcus faecalis , Comportamento Alimentar , Microbioma Gastrointestinal , Comportamento do Lactente , Lactobacillus acidophilus , Probióticos/administração & dosagem , Administração Oral , Bifidobacterium/isolamento & purificação , Método Duplo-Cego , Enterococcus faecalis/isolamento & purificação , Fezes/microbiologia , Feminino , Humanos , Recém-Nascido , Recém-Nascido Prematuro , Lactobacillus acidophilus/isolamento & purificação , Masculino
16.
Blood ; 129(6): 771-782, 2017 02 09.
Artigo em Inglês | MEDLINE | ID: mdl-27956387

RESUMO

Acute myeloid leukemia (AML) is an aggressive malignancy where despite improvements in conventional chemotherapy and bone marrow transplantation, overall survival remains poor. Sphingosine kinase 1 (SPHK1) generates the bioactive lipid sphingosine 1-phosphate (S1P) and has established roles in tumor initiation, progression, and chemotherapy resistance in a wide range of cancers. The role and targeting of SPHK1 in primary AML, however, has not been previously investigated. Here we show that SPHK1 is overexpressed and constitutively activated in primary AML patient blasts but not in normal mononuclear cells. Subsequent targeting of SPHK1 induced caspase-dependent cell death in AML cell lines, primary AML patient blasts, and isolated AML patient leukemic progenitor/stem cells, with negligible effects on normal bone marrow CD34+ progenitors from healthy donors. Furthermore, administration of SPHK1 inhibitors to orthotopic AML patient-derived xenografts reduced tumor burden and prolonged overall survival without affecting murine hematopoiesis. SPHK1 inhibition was associated with reduced survival signaling from S1P receptor 2, resulting in selective downregulation of the prosurvival protein MCL1. Subsequent analysis showed that the combination of BH3 mimetics with either SPHK1 inhibition or S1P receptor 2 antagonism triggered synergistic AML cell death. These results support the notion that SPHK1 is a bona fide therapeutic target for the treatment of AML.


Assuntos
Regulação Leucêmica da Expressão Gênica , Leucemia Mieloide Aguda/tratamento farmacológico , Proteína de Sequência 1 de Leucemia de Células Mieloides/antagonistas & inibidores , Fosfotransferases (Aceptor do Grupo Álcool)/antagonistas & inibidores , Receptores de Lisoesfingolipídeo/antagonistas & inibidores , Clorometilcetonas de Aminoácidos/farmacologia , Amino Álcoois/farmacologia , Animais , Células da Medula Óssea/efeitos dos fármacos , Células da Medula Óssea/metabolismo , Células da Medula Óssea/patologia , Inibidores de Caspase/farmacologia , Caspases/genética , Caspases/metabolismo , Morte Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Feminino , Humanos , Leucemia Mieloide Aguda/genética , Leucemia Mieloide Aguda/mortalidade , Leucemia Mieloide Aguda/patologia , Lisofosfolipídeos/metabolismo , Camundongos , Camundongos Endogâmicos NOD , Terapia de Alvo Molecular , Proteína de Sequência 1 de Leucemia de Células Mieloides/genética , Proteína de Sequência 1 de Leucemia de Células Mieloides/metabolismo , Células-Tronco Neoplásicas/efeitos dos fármacos , Células-Tronco Neoplásicas/metabolismo , Células-Tronco Neoplásicas/patologia , Fosfotransferases (Aceptor do Grupo Álcool)/genética , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Quinolinas/farmacologia , Receptores de Lisoesfingolipídeo/genética , Receptores de Lisoesfingolipídeo/metabolismo , Transdução de Sinais , Esfingosina/análogos & derivados , Esfingosina/metabolismo , Análise de Sobrevida , Ensaios Antitumorais Modelo de Xenoenxerto
17.
J Acoust Soc Am ; 139(6): EL202, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27369173

RESUMO

This paper presents a theoretical study of sound propagation in a duct with a tube array flush-mounted periodically. The underlying wave interaction is analyzed by the transfer matrix method, and validated by the numerical simulation. With identical tubes, a particular situation is observed that the periodic distance and the tube length are "matched," leading to a strong coupling effect by Bragg reflection and tube resonance, resulting in a wide stopband in low frequency. Even when the tubes in the array are not identical, the Bragg reflection can still work to broaden the stopband which was originally contributed by tube resonances.

18.
Front Plant Sci ; 7: 437, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27148281

RESUMO

High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes the structure of high-density linkage maps much more convenient and practical, which simplifies SNP discovery and high-throughput genotyping. In this research, a high-density linkage map of cucumber was structured using specific length amplified fragment sequencing, using 153 F2 populations of S1000 × S1002. The high-density genetic map composed 3,057 SLAFs, including 4,475 SNP markers on seven chromosomes, and spanned 1061.19 cM. The average genetic distance is 0.35 cM. Based on this high-density genome map, QTL analysis was performed on two cucumber fruit traits, fruit length and fruit diameter. There are 15 QTLs for the two fruit traits were detected.

19.
Biomed Rep ; 4(4): 475-478, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27073636

RESUMO

The aim of the present study was to evaluate the serum levels of α-fetoprotein (AFP), free-ß-human chorionic gonadotropin (free-ß-hCG) and unconjugated estriol (uE3) in second trimester maternal serum and comparative analysis of serum markers levels in normal twin and singleton pregnancies, and to evaluate the feasible methods for the screening performances of triple markers in twin pregnancy. The levels of maternal serum markers were measured by magnetic microparticle chemiluminescence immunoassay in the second trimester. TCSoft was used for calculating the risk of Down's syndrome (DS). The marker levels were compared between the twin and singleton pregnancies, along with gestational age, and the weight or age of the pregnant women. The concentrations of the maternal serum markers were higher on average in twin compared to in singleton pregnancies in the second trimester of the gestation. The levels of AFP were 2-fold higher in twin compared to those in singleton pregnancies in weeks 16, 17 and total gestational weeks. For free-ß-hCG, the levels were higher in 15 and 18 gestational weeks. However, significant differences were found in all the gestational weeks for uE3. There was no correlation between the levels of markers and weight or age in the twin pregnancies. The serum marker levels of AFP, free-ß-hCG and uE3 in normal twins were not all 2-fold higher compared to singleton pregnancies. The current singleton gestational age-specific model for DS screening requires further correction so that it is feasible for screening in twin pregnancies.

20.
Mol Genet Genomics ; 290(5): 2007-18, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25952908

RESUMO

Trichomes on plants, similar to fine hairs on animal and human bodies, play important roles in plant survival and development. They also represent a useful model for the study of cell differentiation. Although the regulatory gene network of unicellular trichome development in Arabidopsis thaliana has been well studied, the genes that regulate multicellular trichome development remain unclear. We confirmed that Cucumis sativus (cucumber) trichomes are multicellular and unbranched, but identified a spontaneous mutant, trichome-less (tril), which presented a completely glabrous phenotype. We compared the transcriptome profilings of the tril mutant and wild type using the Illumina HiSeq 2000 sequencing technology. A total of 991 genes exhibited differential expression: 518 were up-regulated and 473 were down-regulated. We further identified 62 differentially expressed genes that encoded crucial transcription factors and were subdivided into seven categories: homeodomain, MADS, MYB, and WRKY domains, ethylene-responsive, zinc finger, and other transcription factor genes. We further analyzed the tissue-expression profiles of two candidate genes, GLABRA2-like and ATHB51-like, using qRT-PCR and found that these two genes were specifically expressed in the epidermis and trichomes, respectively. These results and the tril mutant provide useful tools to study the molecular networks associated with multicellular trichome development.


Assuntos
Genes de Plantas , Transcriptoma , Tricomas/crescimento & desenvolvimento , Núcleo Celular/genética , Cucumis sativus , Microscopia Eletrônica de Varredura , Reação em Cadeia da Polimerase Via Transcriptase Reversa
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