Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 31
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Neurosci Lett ; 718: 134728, 2020 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-31899310

RESUMO

Aggression reflects the psychological and physical behavior that perpetrator intends to harm victim. Initiation of aggression is influenced by the distal factors (e.g. personality) and proximate causes (e.g. affect) of perpetrator. However, few studies explored the brain structural basis of relationship between these traits and aggressive behavior. In this study, we first explored the association between cortical thickness and aggression in a large young adult sample from the Human Connectome Project. Results found aggressive behavior assessed by the Adult Self-Report was positively correlated with cortical thickness in left superior frontal gyrus (SFG), which was implicated in emotion regulation and executive function. Then, mediation analyses with distal and proximate factors separately showcased that the association between the left SFG thickness and aggressive behavior was partially mediated by negative affect (anger and sadness), and fully mediated by personality traits (agreeableness and neuroticism). Taken together, these experimental findings established dorsal prefrontal cortex as the key region in generating aggressive behavior, and gave a neutral explanation for why individuals with high negative affect and neuroticism exhibit more aggression. This study implicated the possible targeted brain region and behavioral intervention for such at-risk individuals initiating violence.

2.
Medicine (Baltimore) ; 98(49): e18162, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31804329

RESUMO

RATIONALE: The prognosis of lung cancer is dismal, which has resulted in lung carcinoma being one of the leading causes of cancer-related deaths worldwide. Non-small cell lung cancer accounts for approximately 80% of all types of lung carcinoma. The skeletal system and central nervous system are the most common distal metastatic sites in patients with lung cancer, while cutaneous and soft tissues metastasis is rare. PATIENT CONCERNS: We report a case of concomitant metastasis in the nasal tip and suspected buttocks metastasis secondary to lung cancer, who complained of repeated cough and white sputum for 6 months. DIAGNOSE: Primary lung cancer was diagnosed by bronchoscopy and biopsy, lesion on nasal tip was confirmed by biopsy. Furthermore, PET-CT scan identified the untouchable buttocks lesion that could have been easily missed. INTERVENTIONS: This patient refused systemic treatments, but he chose traditional Chinese medicine at home. OUTCOMES: He died 6 months after the diagnosis. LESSONS: The possibility of metastasis of primary cancers should be considered when encountering soft-tissue neoplasm lesions, and a biopsy of the suspicious cutaneous lesions could likely aid in the histological identification of the primary cancer. PET-CT scan could be an effective supplementary tool for the diagnosis and evaluation of cancers.


Assuntos
Neoplasias Pulmonares/patologia , Neoplasias Cutâneas/secundário , Neoplasias de Tecidos Moles/secundário , Humanos , Neoplasias Pulmonares/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons
3.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(8): 949-952, 2019 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-31537217

RESUMO

OBJECTIVE: To explore the effect of intensive insulin therapy (IIT) on high mobility group box-1/nuclear factor-ΚB (HMGB1/NF-ΚB) signaling pathway in severe traumatic brain injury (sTBI) patient with stress hyperglycemia. METHODS: Sixty-one sTBI patients with stress hyperglycemia [Glasgow coma scale (GCS) ≤ 8, three times of random blood glucose levels > 11.1 mmoL/L, glycosylated hemoglobin (HbA1c) < 0.065] admitted to the Affiliated Huaian No.1 People's Hospital of Nanjing Medical University from July 2015 to October 2017 were enrolled. Patients were divided into IIT group (29 cases, keeping blood glucose at 4.4-7.8 mmol/L) and conventional glycemic therapy (CGT) group (32 cases, keeping blood glucose at 7.8-12.2 mmo/L) according to the random number table method. Before treatment and 1, 7 and 14 days after treatment, the levels of plasma HMGB1 and tumor necrosis factor-α (TNF-α) were measured by enzyme linked immunosorbent assay (ELISA); C-reactive protein (CRP) was determined by automatic biochemical analyzer, and NF-ΚB p65 gene expression in peripheral blood mononuclear cells was detected by real-time quantitative polymerase chain reaction (PCR). RESULTS: Nine patients were withdrawn from the observation because the 4 consecutive blood glucose monitoring did not reach the target value, combined with severe infection, or abandoned the treatment with serious brain damage. Finally, 52 patients were enrolled in the analysis, including 28 in CGT group and 24 in IIT group. The levels of plasma HMGB1, TNF-α, CRP and the expression of NF-ΚB gene in monocytes of the two groups at 1 day after treatment were significantly higher than those before treatment, and reached the peak value, then gradually decreased. After 7 days of treatment, they were significantly lower than 1 day. The levels of plasma CRP and TNF-α in the IIT group were significantly lower than those in the CGT group [CRP (mg/L): 36.7±4.4 vs. 45.1±6.1, TNF-α (ng/L): 42.4±9.7 vs. 53.2±9.1, both P < 0.05], the level of HMGB1 in plasma and the expression of NF-ΚB p65 in monocytes were significantly lower than those in the CGT group after 14 days of treatment [HMGB1 (µg/L): 60.1±8.7 vs. 80.5±9.1, NF-ΚB p65 (ΔCt): 35.8±8.5 vs. 53.5±7.3, both P < 0.05]. CONCLUSIONS: IIT inhibits the inflammatory response in sTBI patients with stress hyperglycemia through HMGB1/NF-ΚB pathway.


Assuntos
Lesões Encefálicas Traumáticas/metabolismo , Proteína HMGB1/metabolismo , Hiperglicemia/metabolismo , Insulina/uso terapêutico , NF-kappa B/metabolismo , Glicemia , Automonitorização da Glicemia , Humanos , Leucócitos Mononucleares , Fator de Necrose Tumoral alfa
4.
Biochim Biophys Acta Gen Subj ; 1863(9): 1360-1370, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31170498

RESUMO

BACKGROUND: Renal fibrosis is a common pathological hallmark of chronic kidney disease, and no effective treatment is clinically available to manage its progression. Astaxanthin was recently found to be anti-fibrotic, but its effect on renal fibrosis remains unclear. METHODS: C57BL/6J mice were subjected to unilateral ureteral obstruction and intragastrically administered astaxanthin. Histopathology and immunohistochemistry were performed to evaluate renal fibrosis. Flow cytometry was used to examine lymphocyte accumulation in the fibrotic kidneys. Western blotting, real-time qPCR, and immunofluorescence were performed to cover the underlying mechanism concerning astaxanthin treatment during renal fibrosis. RESULTS: Oral administration of astaxanthin effectively alleviates renal fibrosis in mice. In vitro, astaxanthin inhibited fibroblast activation by modulating Smad2, Akt and STAT3 pathways and suppressed epithelial-to-mesenchymal transition in renal tubular epithelial cells through Smad2, snail, and ß-catenin. Moreover, astaxanthin significantly induced the rapid accumulation of CD8+ T cells in fibrotic kidneys, which was accompanied by elevated expression of IFN-γ. Accordingly, the depletion of CD8+ T cells strongly diminished the protective effect of astaxanthin. Further investigation showed that astaxanthin increased the population of CD8+ T cells by upregulating the expression of CCL5 in macrophages. CONCLUSIONS: These findings highlight the beneficial effect of astaxanthin on fibroblast activation, epithelial-to-mesenchymal transition, and CD8+ T cell recruitment during renal fibrosis. GENERAL SIGNIFICANCE: These data indicate that astaxanthin could serve as a therapeutic strategy to treat renal fibrotic conditions.

5.
Neuroscience ; 414: 210-218, 2019 08 21.
Artigo em Inglês | MEDLINE | ID: mdl-31173807

RESUMO

Subjective well-being (SWB) is closely related to our physical and mental health. Existing studies show that neural or genetic basis underpins individual difference in SWB. Moreover, researchers have found high enrichment of SWB-related mutations in the central nervous system, but the relationship between the genetic architecture of SWB and brain morphology has not been explored. Considering the polygenic nature of SWB, in this study, we aim to establish a measure of additive genetic effect on SWB and explore its relationship to the brain anatomical structure. Based on the results of genome-wide association study (GWAS) on SWB, the polygenic scores (PGSs) of SWB at eight different thresholds were calculated in a large Chinese sample (N = 585). Then, we analyzed the associations between the PGSs of SWB and cortical thickness (CT) or gray matter volume (GMV) measured from 3.0-T structural imaging data. In whole-brain analyses, we found that a higher PGS was significantly associated with increased CT in the right superior temporal gyrus (STG) and GMV in the right insula, both of which are involved in social cognition and emotional processing. More importantly, these findings were repeatable at some different thresholds. The results may suggest that the brain morphology of right STG and insula is partly regulated by SWB-related genetic factors.

6.
Medicine (Baltimore) ; 98(18): e15383, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31045788

RESUMO

BACKGROUND: Neuroprotective effects of dexmedetomidine are reported in preclinical and clinical studies but evidence regarding the postoperative neurocognitive function is still unclear. This study performed a meta-analysis on outcomes of studies which examined neurocognitive performance and inflammatory factors to investigate the effects of dexmedetomidine on postoperative cognitive dysfunction (POCD) and inflammation in patients after general anaesthesia. METHODS: Literatures were searched in several electronic databases and studies were selected by following precise inclusion criteria. We searched PubMed, EMBASE, the Cochrane Library, China Academic Journals full-text database (CNKI), and Google Scholar to find randomized controlled trials (RCTs) of the influence of dexmedetomidine on POCD and inflammation in patients who had undergone general anaesthesia. Two researchers independently screened the literature, extracted data, and evaluated quality of methodology against inclusion and exclusion criteria. Meta-analyses of pooled ORs of POCD incidences and mean differences in neurocognitive assessment scores and inflammation levels were carried out and subgroup analyses were performed. Stata 12.0 was used to conduct our meta-analysis. RESULTS: Twenty-six RCTs were included. Compared with controls, perioperative dexmedetomidine treatment significantly reduced the incidence of POCD (pooled ORs = 0.59, 95% confidence interval (CI) 0.45-2.95) and improved Mini-Mental State Examination (MMSE) score (standardized mean difference (SMD) = 1.74, 95% CI 0.43-3.05) on the first postoperative day. Furthermore, perioperative dexmedetomidine treatment significantly decreased IL-6 (SMD = -1.31, 95% CI -1.87-0.75, P < .001) and TNF-α (SMD = -2.14, 95% CI -3.14-1.14, P < .001) compared to saline/comparators treatment. In the stratified analysis by surgical type, age, type of control, and study region, the differences were also significant between dexmedetomidine- and saline-treated patients. CONCLUSION: Perioperative dexmedetomidine treatment is associated with significantly reduced incidence of POCD and inflammation and better neurocognitive function postoperatively in comparison with both saline controls and comparator anaesthetics.


Assuntos
Agonistas de Receptores Adrenérgicos alfa 2/administração & dosagem , Anestesia Geral/efeitos adversos , Disfunção Cognitiva/induzido quimicamente , Disfunção Cognitiva/prevenção & controle , Dexmedetomidina/administração & dosagem , China , Humanos , Inflamação/tratamento farmacológico , Mediadores da Inflamação/metabolismo , Interleucina-6/biossíntese , Testes de Estado Mental e Demência , Razão de Chances , Período Perioperatório , Complicações Pós-Operatórias/induzido quimicamente , Complicações Pós-Operatórias/prevenção & controle , Ensaios Clínicos Controlados Aleatórios como Assunto , Fator de Necrose Tumoral alfa/biossíntese
7.
J Clin Gastroenterol ; 53(7): e276-e283, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29912754

RESUMO

BACKGROUND: Computed tomography-measured visceral adipose tissue (VAT) and the distribution of VAT are highly correlated with the severity and prognosis of acute pancreatitis (AP). To date, all available data are from the overall AP patient population; no subgroup analysis has been conducted to evaluate patients with moderately severe AP or patients with hyperlipidemia acute pancreatitis (HLAP) as independent populations. Currently, studies on the relationship between VAT and HLAP are lacking. MATERIALS AND METHODS: A total of 235 patients with moderately severe AP or severe acute pancreatitis were divided into 2 groups according to whether hyperlipidemia was present: the HLAP group and the non-HLAP group. The general inpatient information was collected, and computed tomography was used to measure VAT, subcutaneous adipose tissue (SAT), total adipose tissue, and VAT/SAT (V/S). The data were subjected to t test, χ test, matrix scatter plot, logistic regression, and receiver operating characteristic analyses to evaluate the relationship between VAT and HLAP severity. RESULTS: Significant differences were observed in VAT, SAT, total adipose tissue, and triglycerides (TGs) between the HLAP group and the non-HLAP group (P<0.001). Significant correlations were observed between VAT and body mass index (r=0.425, P=0.017) and between VAT and TG (r=0.367, P=0.042). In the HLAP group, VAT, V/S, TG, and local complications may have significant effects on disease severity. The receiver operating characteristic curves showed that VAT and V/S were more reliable than TGs in evaluating disease severity [area under the curve (AUC) of VAT: 0.819, P<0.001; AUC of V/S: 0.855, P<0.001; AUC of TG: 0.671, P=0.04]. Disease severity was reliably evaluated at 139 cm, the cut-off value of VAT. The cut-off value of V/S was 1.145; high V/S was associated with extended intensive care unit stay. VAT and its distribution had no significant effects on mortality. CONCLUSIONS: For patients with moderately severe to severe HLAP, VAT was correlated with body mass index and TG. VAT and V/S were valuable factors for evaluating disease severity and prognosis. However, VAT had no effect on mortality, and VAT could not be used to evaluate patients with moderately severe to severe non-HLAP.

8.
Sci Rep ; 8(1): 15975, 2018 10 29.
Artigo em Inglês | MEDLINE | ID: mdl-30374043

RESUMO

Mind-wandering is omnipresent in our lives. The benefits of mind-wandering are not yet clear, but given how much time we spend mind-wandering, this mental function is likely to be important. Accordingly, it is essential to understand the neural and cognitive mechanisms of mind-wandering. In a recent study by the leading author of the present paper it was demonstrated that by applying transcranial direct current stimulation (tDCS) of the frontal lobes, but not sham or occipital cortex stimulation, it was possible to increase propensity of mind-wandering. The goal of the present study has been to replicate these previous findings and to extend them by examining whether changes in mind-wandering as a result of stimulation are associated with a change of meta-awareness of the attentional focus. By using a larger sample size and by conducting the experiment in a different country and language, we fully replicated the key original findings by showing that stimulation of the prefrontal cortex increased the level of mind-wandering. We also show that stimulation had no major effect on the level of meta-awareness of the attentional focus. Taken together, our results indicate that mind-wandering - probably the most internal and self-related mental function - can be modulated externally, that at least in some cases mind-wandering might not be regulated by meta-awareness, and that the frontal lobes might play a causal role in mind-wandering.


Assuntos
Atenção/fisiologia , Conscientização/fisiologia , Lobo Frontal/fisiologia , Feminino , Humanos , Masculino , Análise e Desempenho de Tarefas , Estimulação Transcraniana por Corrente Contínua , Adulto Jovem
9.
Front Microbiol ; 9: 2436, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30364071

RESUMO

The development of lignocellulosic bioethanol plays an important role in the substitution of petrochemical energy and high-value utilization of agricultural wastes. The safe and stable expression of cellulase gene sestc was achieved by applying the clustered regularly interspaced short palindromic repeats-Cas9 approach to the integration of sestc expression cassette containing Agaricus biporus glyceraldehyde-3-phosphate-dehydrogenase gene (gpd) promoter in the Saccharomyces cerevisiae chromosome. The target insertion site was found to be located in the S. cerevisiae hexokinase 2 by designing a gRNA expression vector. The recombinant SESTC protein exhibited a size of approximately 44 kDa in the engineered S. cerevisiae. By using orange peel as the fermentation substrate, the filter paper, endo-1,4-ß-glucanase, exo-1,4-ß-glucanase activities of the transformants were 1.06, 337.42, and 1.36 U/mL, which were 35.3-fold, 23.03-fold, and 17-fold higher than those from wild-type S. cerevisiae, respectively. After 6 h treatment, approximately 20 g/L glucose was obtained. Under anaerobic conditions the highest ethanol concentration reached 7.53 g/L after 48 h fermentation and was 37.7-fold higher than that of wild-type S. cerevisiae (0.2 g/L). The engineered strains may provide a valuable material for the development of lignocellulosic ethanol.

10.
Biochim Biophys Acta Mol Basis Dis ; 1864(11): 3824-3836, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30251698

RESUMO

Sphingosine kinases (Sphks) are the rate-limiting enzymes in the conversion of sphingosine to biologically active sphingosine-1-phosphate. The present study aimed to determine the role of Sphk2 and its downstream targets in renal fibroblast activation and interstitial fibrosis. In the kidney interstitium of patients with renal fibrosis, Sphk2high-expressing cells (mainly interstitial fibroblasts) were significantly elevated and highly correlated with disease progression in patients. In a murine model of renal interstitial fibrosis, Sphk2 was upregulated in the kidney of wild-type mice in response to disease progression. Importantly, Sphk2-knockout (KO) mice exhibited significantly lower levels of extracellular matrix (ECM) production and a suppressed inflammatory response in the kidney tissues, compared to those in their wild-type counterparts, whereas the expression of TGF-ß1 was unaffected. TGF-ß1 effectively upregulated Sphk2 expression in the renal interstitial fibroblast line, NRK-49F, independent of canonical Smad signaling activation. Furthermore, siRNA-mediated Sphk2 knockdown or suppression of Sphk2 activity by ABC294640 exposure effectively attenuated AKT and STAT3 activation and ECM production, but had no effects on Smad2 and Smad3 activation. Sphk2 phosphorylated Fyn to activate downstream STAT3 and AKT, thereby promoting ECM synthesis. Therefore, our findings indicate that targeting Sphk2-Fyn-STAT3/AKT signaling pathway may be a novel therapeutic approach for renal fibrosis.


Assuntos
Fibroblastos/imunologia , Rim/patologia , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Proteínas Proto-Oncogênicas c-fyn/metabolismo , Insuficiência Renal Crônica/patologia , Animais , Linhagem Celular , Modelos Animais de Doenças , Matriz Extracelular/imunologia , Matriz Extracelular/patologia , Fibroblastos/patologia , Fibrose , Células HEK293 , Humanos , Rim/imunologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fosfotransferases (Aceptor do Grupo Álcool)/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ratos , Insuficiência Renal Crônica/imunologia , Fator de Transcrição STAT3/metabolismo , Transdução de Sinais/imunologia , Fator de Crescimento Transformador beta1/imunologia , Fator de Crescimento Transformador beta1/metabolismo , Regulação para Cima
11.
Front Psychol ; 9: 925, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29962984

RESUMO

Little is known about the association between emotional intelligence (EI) and trait creativity (TC), and the brain structural bases which involves. This study investigated the neuroanatomical basis of the association between EI and TC which measured by the Schutte self-report EI scale and the Williams creativity aptitude test. First, the voxel-based morphometry (VBM) analysis was used to explore the brain structures which is closely related to EI in a large young sample (n = 213). The results showed that EI was positively correlated with the regional gray matter volume (rGMV) in the right orbitofrontal cortex (OFC), which is regarded as a key region of emotional processing. More importantly, further mediation analysis revealed that rGMV in the right OFC partially mediated the association between EI and TC, which showed the OFC volume could account for the relationship between EI and TC. These findings confirmed the close relationship between EI and TC, and highlighted that the brain volumetric variation in the OFC associated with the top-down processing of emotion regulation, which may play a critical role in the promotion of TC. Together, these findings contributed to sharpening the understanding of the complex relationship between EI and TC from the perspective of brain structural basis.

12.
Neurosci Lett ; 683: 104-110, 2018 09 14.
Artigo em Inglês | MEDLINE | ID: mdl-29936269

RESUMO

Creativity is necessary to human survival, human prosperity, civilization and well-being. Visual creativity is an important part of creativity and is the ability to create products of novel and useful visual forms, playing important role in many fields such as art, painting and sculpture. There have been several neuroimaging studies exploring the neural basis of visual creativity. However, to date, little is known about the relationship between cortical structure and visual creativity as measured by the Torrance Tests of Creative Thinking. Here, we investigated the association between cortical thickness and visual creativity in a large sample of 310 healthy adults. We used multiple regression to analyze the correlation between cortical thickness and visual creativity, adjusting for gender, age and general intelligence. The results showed that visual creativity was significantly negatively correlated with cortical thickness in the left middle frontal gyrus (MFG), right inferior frontal gyrus (IFG), right supplementary motor cortex (SMA) and the left insula. These observations have implications for understanding that a thinner prefrontal cortex (PFC) (e.g. IFG, MFG), SMA and insula correspond to higher visual creative performance, presumably due to their role in executive attention, cognitive control, motor planning and dynamic switching.


Assuntos
Arte , Córtex Cerebral/diagnóstico por imagem , Córtex Cerebral/fisiologia , Criatividade , Pensamento/fisiologia , Adolescente , Adulto , Mapeamento Encefálico/métodos , Feminino , Humanos , Imagem por Ressonância Magnética/métodos , Masculino , Tamanho do Órgão , Adulto Jovem
13.
J Neuroinflammation ; 15(1): 136, 2018 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-29728120

RESUMO

BACKGROUND: Interleukin-33 (IL-33) is increasingly being recognized as a key immunomodulatory cytokine in many neurological diseases. METHODS: In the present study, wild-type (WT) and IL-33-/- mice received intracerebroventricular (i.c.v.) injection of lipopolysaccharide (LPS) to induce neuroinflammation. Intravital microscopy was employed to examine leukocyte-endothelial interactions in the brain vasculature. The degree of neutrophil infiltration was determined by myeloperoxidase (MPO) staining. Real-time PCR and western blotting were used to detect endothelial activation. Enzyme-linked immunosorbent assay and quantitative PCR were conducted to detect pro-inflammatory cytokine levels in the brain. RESULTS: In IL-33-/- mice, neutrophil infiltration in the brain cortex and leukocyte-endothelial cell interactions in the cerebral microvessels were significantly decreased as compared to WT mice after LPS injection. In addition, IL-33-/- mice showed reduced activation of microglia and cerebral endothelial cells. In vitro results indicated that IL-33 directly activated cerebral endothelial cells and promoted pro-inflammatory cytokine production in LPS-stimulated microglia. CONCLUSIONS: Our study indicated that IL-33/ST2 signaling plays an important role in the activation of microglia and cerebral endothelial cells and, therefore, is essential in leukocyte recruitment in brain inflammation. The role of IL-33/ST2 in LPS induced neuroinflammation.


Assuntos
Encefalite/metabolismo , Células Endoteliais/metabolismo , Proteína 1 Semelhante a Receptor de Interleucina-1/biossíntese , Interleucina-33/biossíntese , Microglia/metabolismo , Animais , Animais Recém-Nascidos , Células Cultivadas , Encefalite/induzido quimicamente , Encefalite/imunologia , Células Endoteliais/imunologia , Imunidade Inata/fisiologia , Injeções Intraventriculares , Proteína 1 Semelhante a Receptor de Interleucina-1/imunologia , Interleucina-33/imunologia , Lipopolissacarídeos/toxicidade , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microglia/imunologia , Infiltração de Neutrófilos/fisiologia
14.
Sensors (Basel) ; 18(5)2018 May 08.
Artigo em Inglês | MEDLINE | ID: mdl-29738512

RESUMO

Nonrigid multimodal image registration remains a challenging task in medical image processing and analysis. The structural representation (SR)-based registration methods have attracted much attention recently. However, the existing SR methods cannot provide satisfactory registration accuracy due to the utilization of hand-designed features for structural representation. To address this problem, the structural representation method based on the improved version of the simple deep learning network named PCANet is proposed for medical image registration. In the proposed method, PCANet is firstly trained on numerous medical images to learn convolution kernels for this network. Then, a pair of input medical images to be registered is processed by the learned PCANet. The features extracted by various layers in the PCANet are fused to produce multilevel features. The structural representation images are constructed for two input images based on nonlinear transformation of these multilevel features. The Euclidean distance between structural representation images is calculated and used as the similarity metrics. The objective function defined by the similarity metrics is optimized by L-BFGS method to obtain parameters of the free-form deformation (FFD) model. Extensive experiments on simulated and real multimodal image datasets show that compared with the state-of-the-art registration methods, such as modality-independent neighborhood descriptor (MIND), normalized mutual information (NMI), Weber local descriptor (WLD), and the sum of squared differences on entropy images (ESSD), the proposed method provides better registration performance in terms of target registration error (TRE) and subjective human vision.

15.
Neuroscience ; 384: 406-416, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-29792905

RESUMO

Life satisfaction reflects an individual's general evaluation of their overall quality of life. It has been hypothesized that relationship status (i.e. state of intimate relationship such as marriage, unmarried cohabiting, dating with others, single or divorce) may influence individual life satisfaction. However, there is little accessible empirical evidence that allows us to explore this proposition. Using a large sample of young adults (n = 1031) from the Human Connectome Project (HCP), we showed that compared to other relationship statuses (e.g., individuals who were single or divorced, individuals who dated others, and etcetera), marriage/cohabitation subjects not only revealed a higher life satisfaction plus higher emotional and instrumental support, but also reduced perceived stress, which contributed to higher life satisfaction. Using general linear model with cortical thickness as the dependent variable, life satisfaction was negatively associated with the left superior frontal gyrus (SFG) and bilateral middle frontal gyrus (MFG). Interestingly, both right MFG and left SFG could interact with relationship status to predict self-reported life satisfaction, in addition to being associated with a much lower life satisfaction in non-married/cohabiting individuals. These effects were independent of emotional, instrumental support, and socioeconomic status. Besides, statistical significance of the moderation effect pertaining to relationship status was lost once perceived stress was included as a covariate into the moderation model. Our findings provided empirical evidence for the potentially positive role of relationship status in life satisfaction, and also showed that remission of stress may be a critical factor.


Assuntos
Encéfalo/diagnóstico por imagem , Conectoma , Felicidade , Casamento/psicologia , Satisfação Pessoal , Qualidade de Vida/psicologia , Adulto , Feminino , Humanos , Imagem por Ressonância Magnética , Masculino , Autorrelato , Apoio Social , Estresse Psicológico/diagnóstico por imagem , Estresse Psicológico/psicologia , Adulto Jovem
16.
World J Microbiol Biotechnol ; 34(5): 65, 2018 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-29687334

RESUMO

D-Allulose as a low-energy and special bioactive monosaccharide sugar is essential for human health. In this study, the D-psicose-3-epimerase gene (DPEase) of Agrobacterium tumefaciens was transferred into thermotolerant Kluyveromyces marxianus to decrease the production cost of D-allulose and reduce the number of manufacturing procedures. The cell regeneration of K. marxianus and cyclic catalysis via whole-cell reaction were investigated to achieve the sustainable application of K. marxianus and the consumption of residual D-fructose. Results showed that DPEase, encoding a 33 kDa protein, could be effectively expressed in thermotolerant K. marxianus. The engineered K. marxianus produced 190 g L-1 D-allulose with 750 g L-1 D-fructose as a substrate at 55 °C within 12 h. Approximately 100 g of residual D-fructose was converted into 34 g of ethanol, and 15 g of the engineered K. marxianus cells was regenerated after fermentation at 37 °C for 21 h. The purity of D-allulose of more than 90% could be obtained without isolating it from D-allulose and D-fructose mixture through residual D-fructose consumption. This study provided a valuable pathway to regenerate engineered K. marxianus cells and achieve cyclic catalysis for D-allulose production.


Assuntos
Agrobacterium tumefaciens/enzimologia , Agrobacterium tumefaciens/genética , Carboidratos Epimerases/genética , Carboidratos Epimerases/metabolismo , Frutose/metabolismo , Kluyveromyces/genética , Kluyveromyces/fisiologia , Regeneração , Catálise , Clonagem Molecular , Estabilidade Enzimática , Etanol/metabolismo , Fermentação , Regulação Enzimológica da Expressão Gênica , Vetores Genéticos , Concentração de Íons de Hidrogênio , Kluyveromyces/crescimento & desenvolvimento , Engenharia Metabólica , Temperatura Ambiente , Fatores de Tempo
17.
Talanta ; 182: 142-147, 2018 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-29501133

RESUMO

In this paper, we describe a novel, label-free and nicking enzyme-assisted fluorescence signal amplification strategy that demonstrates to be cost efficient, sensitive, and unique for assaying the RNase H activity and inhibition based on G-quadruplex formation using a thioflavin T (ThT) dye. This novel assay method is able to detect RNase H with a detection limit of 0.03 U /mL and further exhibits a good linearity R2 = 0.9923 at a concentration range of 0.03-1 U/mL under optimized conditions. Moreover, the inhibition effect of gentamycin on the RNase H activity is also studied. This strategy provides a potential tool for the biochemical enzyme analysis and inhibitor screening.


Assuntos
Bioensaio , Desoxirribonucleases de Sítio Específico do Tipo II/química , Corantes Fluorescentes/química , Quadruplex G , Ribonuclease H/sangue , Tiazóis/química , Benzotiazóis , DNA de Cadeia Simples/síntese química , DNA de Cadeia Simples/química , Fluorescência , Gentamicinas/química , Humanos , Sequências Repetidas Invertidas , Limite de Detecção , Oligonucleotídeos/síntese química , Oligonucleotídeos/química , Inibidores da Síntese de Proteínas/química , RNA/síntese química , RNA/química , Ribonuclease H/antagonistas & inibidores , Espectrometria de Fluorescência/métodos
18.
Brain Behav Immun ; 70: 293-304, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29548997

RESUMO

Neuropsychiatric (NP) involvement in systemic lupus erythematosus (SLE) severely impacts patients' quality of life and leads to a poor prognosis. The current therapeutic protocol, corticosteroid administration, can also induce neuropsychiatric disorders. FTY720 is an immunomodulator that selectively confines lymphocytes in lymph nodes and reduces autoreactive T cell recruitment to the central nervous system (CNS). This study aimed to identify a novel therapeutic strategy for NPSLE. B6.MRL-lpr mice were treated with oral administration of FTY720 (2 mg/kg) three times per week for 12 weeks, to evaluate its efficacy in a model of NPSLE. FTY720 significantly attenuated the impulsive and depression-like behavior of B6.MRL-lpr mice. Neuronal damage was reduced in the cortex, hippocampus, and amygdala of the FTY720-treated B6.MRL-lpr mice, as well as in TNF-α-treated HT22 cells. Additionally, FTY720 downregulated levels of inflammatory cytokines, and reduced the infiltration of T cells and neutrophils in the brain parenchyma. FTY720 also acted directly on cerebral endothelial cells and reduced the permeability of the blood-brain barrier (BBB) in B6.MRL-lpr mice, as evidenced by reduced central IgG and albumin levels. Finally, FTY720 significantly inhibited activation of PI3K/Akt/GSK3ß/p65 signaling, which further reduced the expression levels of adhesion molecules in bEND.3 cells treated with B6.MRL-lpr mouse serum. Collectively, our data indicate that oral administration of FTY720 at an early stage has beneficial effects in NPSLE-model B6.MRL-lpr mice, suggesting that it may represent an effective new therapeutic strategy for NPSLE.


Assuntos
Cloridrato de Fingolimode/farmacologia , Lúpus Eritematoso Sistêmico/imunologia , Animais , Encéfalo , Citocinas , Depressão , Modelos Animais de Doenças , Feminino , Cloridrato de Fingolimode/uso terapêutico , Fatores Imunológicos/uso terapêutico , Lúpus Eritematoso Sistêmico/terapia , Linfócitos/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos MRL lpr , Qualidade de Vida , Transdução de Sinais , Linfócitos T
19.
J Leukoc Biol ; 103(1): 107-118, 2018 01.
Artigo em Inglês | MEDLINE | ID: mdl-29345065

RESUMO

Fingolimod (FTY720), an immunomodulator, is approved as an oral treatment for patients with relapsing forms of multiple sclerosis. Its effects are largely attributed to its mechanism of selectively retaining lymphocytes in the lymph nodes to reduce autoreactive T-cell recruitment in the CNS. In this study, we investigated the therapeutic effect of FTY720 on an animal model of CNS inflammation induced by intracerebral ventricle LPS injection. We found that FTY720 treatment significantly prevented LPS-induced neutrophil recruitment in the CNS by inhibiting leukocyte recruitment in cerebral microvessels. Furthermore, FTY720 also inhibited the expressions of adhesion molecules on the cerebral endothelium, but did not affect the expression levels of pro-inflammatory cytokines (TNF-α and IL-6) and chemokines (CXCL1 and CXCL2) in the CNS parenchyma. The inhibition of endothelial activation was accompanied by reduced phosphorylation of signaling molecules, including serine/threonine-specific protein kinase (Akt), STAT6, and nuclear factor-κB. This FTY720-attenuated inhibition of leukocyte recruitment and endothelial activation was reversed by blocking the functions of sphingosine kinase 2 or sphingosine-1-phosphate receptor 1. Our study demonstrated, for the first time, that FTY720 directly inhibits the phosphorylation of multiple signaling molecules in endothelial cells, thereby effectively blocking leukocyte recruitment in the CNS.


Assuntos
Sistema Nervoso Central/imunologia , Cérebro/imunologia , Endotélio Vascular/imunologia , Cloridrato de Fingolimode/farmacologia , Inflamação/imunologia , Leucócitos/imunologia , Microvasos/imunologia , Animais , Células Cultivadas , Sistema Nervoso Central/efeitos dos fármacos , Sistema Nervoso Central/metabolismo , Circulação Cerebrovascular , Cérebro/efeitos dos fármacos , Cérebro/metabolismo , Citocinas/metabolismo , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/metabolismo , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Leucócitos/efeitos dos fármacos , Leucócitos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microvasos/efeitos dos fármacos , Microvasos/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Linfócitos T/efeitos dos fármacos , Linfócitos T/imunologia , Linfócitos T/metabolismo
20.
Cytokine ; 106: 80-88, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29111086

RESUMO

BACKGROUND: Several studies suggest IL-17 is involved in the pathogenesis of organ fibrosis. The exact role of IL-17 in renal interstitial fibrosis has not been fully elucidated. METHODS: We compared the histopathology of renal fibrosis as well as profibrotic TGF-ß signaling in wild-type (WT) and IL-17 knock-out (IL-17-/-) mice using UUO as the disease model. To find out the possible mechanisms involved in the exacerbated renal fibrosis happened to IL-17-/- mice, we analyzed the pattern of ECM synthesis by different fibroblasts cultured with IL-17 and associated signaling mediators. RESULTS: On day3 and day7, IL-17-/- mice developed more severe renal fibrosis compared with WT mice. IL-17 had an inhibitory factor in TGF-ß-induced renal fibroblast activation and ECM synthesis, and sequentially in renal interstitial fibrosis, via down-regulation of Smad -independent pathway (p38MAPK and AKT phosphorylations). CONCLUSION: IL-17 acts an inhibitory factor in TGF-ß-induced renal fibroblast activation and ECM synthesis, and sequentially in renal interstitial fibrosis, via down-regulation of Smad-independent pathway (p38MAPK and AKT phosphorylations). Clarifying the novel regulatory mechanisms of fibrosis by the cytokine IL-17 may lead to a new therapeutic approach for progressive renal disease and fibrosis.


Assuntos
Interleucina-17/metabolismo , Rim/metabolismo , Rim/patologia , Transdução de Sinais , Fator de Crescimento Transformador beta/metabolismo , Animais , Matriz Extracelular/metabolismo , Feto/patologia , Fibroblastos/metabolismo , Fibroblastos/patologia , Fibrose , Mediadores da Inflamação/metabolismo , Interleucina-17/deficiência , Pulmão/patologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Obstrução Ureteral/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA