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2.
Arch Oral Biol ; 133: 105312, 2021 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-34808514

RESUMO

OBJECTIVE: RNA-activated protein kinase-like ER-resident kinase (PERK) was a major transducer of Endoplasmic reticulum (ER) stress response and it directly phosphorylated α-subunit of eukaryotic initiation factor 2 (eIF2α), which specifically promoted the translation of activating transcription factor 4 (ATF4), an important transcription factor in cells' differentiation. The purpose of this study was to establish whether ER stress mediated by PERK-eIF2α-ATF4 pathway was involved in odontoblastic differentiation of human dental pulp cells (DPCs). METHODS: DPCs were isolated from extracted teeth and cultured in odontogenic medium. A recombinant lentiviral vector was constructed to transfect DPCs for PERK knockdown. Alkaline phosphatase (ALP) and Alizarin red S staining were used to characterize the odontoblastic differentiation. Real-time polymerase chain reactions (RT-PCR) were performed to analyze the genes' expressions in DPCs' odontoblastic differentiation. The mRNA and protein levels of ER stress markers were examined by RT-PCR and western blot. RESULTS: DPCs cultured in odontogenic media showed increased ALP activity and mineralized nodule formation. Notably, treatment with differentiation medium resulted in the up-regulation of genes, such as osteocalcin (OCN), bone sialoprotein (BSP), dentin sialophosphoprotein (DSPP), splicing x-box binding protein-1 (sXBP1), ATF4 and glucose-regulated protein 78 (GRP78). Meanwhile, the expressions of PERK-eIF2α-ATF4 pathway proteins, phosphorylated PERK, phosphorylated eIF2α and ATF4, increased in odontoblastic induction cells compared with controls. Furthermore, inhibition of PERK (PERK knockdown) decreased ALP activity and matrix mineralization in DPCs accompanied by the decrease expression of phosphorylated eIF2α and ATF4. CONCLUSION: These results suggested that PERK-eIF2α-ATF4 pathway was involved in the odontoblastic differentiation of DPCs.

3.
Int J Oral Sci ; 13(1): 32, 2021 09 30.
Artigo em Inglês | MEDLINE | ID: mdl-34593752

RESUMO

Oligodontia is the congenital absence of six or more teeth and comprises the more severe forms of tooth agenesis. Many genes have been implicated in the etiology of tooth agenesis, which is highly variable in its clinical presentation. The purpose of this study was to identify associations between genetic mutations and clinical features of oligodontia patients. An online systematic search of papers published from January 1992 to June 2021 identified 381 oligodontia cases meeting the eligibility criteria of causative gene mutation, phenotype description, and radiographic records. Additionally, ten families with oligodontia were recruited and their genetic etiologies were determined by whole-exome sequence analyses. We identified a novel mutation in WNT10A (c.99_105dup) and eight previously reported mutations in WNT10A (c.433 G > A; c.682 T > A; c.318 C > G; c.511.C > T; c.321 C > A), EDAR (c.581 C > T), and LRP6 (c.1003 C > T, c.2747 G > T). Collectively, 20 different causative genes were implicated among those 393 cases with oligodontia. For each causative gene, the mean number of missing teeth per case and the frequency of teeth missing at each position were calculated. Genotype-phenotype correlation analysis indicated that molars agenesis is more likely linked to PAX9 mutations, mandibular first premolar agenesis is least associated with PAX9 mutations. Mandibular incisors and maxillary lateral incisor agenesis are most closely linked to EDA mutations.


Assuntos
Proteínas Wnt , Humanos , Fenótipo
4.
Math Biosci Eng ; 18(5): 6978-6994, 2021 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-34517567

RESUMO

Computer Assisted Diagnosis (CAD) based on brain Magnetic Resonance Imaging (MRI) is a popular research field for the computer science and medical engineering. Traditional machine learning and deep learning methods were employed in the classification of brain MRI images in the previous studies. However, the current algorithms rarely take into consideration the influence of multi-scale brain connectivity disorders on some mental diseases. To improve this defect, a deep learning structure was proposed based on MRI images, which was designed to consider the brain's connections at different sizes and the attention of connections. In this work, a Multiscale View (MV) module was proposed, which was designed to detect multi-scale brain network disorders. On the basis of the MV module, the path attention module was also proposed to simulate the attention selection of the parallel paths in the MV module. Based on the two modules, we proposed a 3D Multiscale View Convolutional Neural Network with Attention (3D MVA-CNN) for classification of MRI images for mental disease. The proposed method outperformed the previous 3D CNN structures in the structural MRI data of ADHD-200 and the functional MRI data of schizophrenia. Finally, we also proposed a preliminary framework for clinical application using 3D CNN, and discussed its limitations on data accessing and reliability. This work promoted the assisted diagnosis of mental diseases based on deep learning and provided a novel 3D CNN method based on MRI data.


Assuntos
Imageamento por Ressonância Magnética , Redes Neurais de Computação , Atenção , Encéfalo/diagnóstico por imagem , Reprodutibilidade dos Testes
5.
Materials (Basel) ; 14(16)2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34443206

RESUMO

The purpose of this research was to make full use of waste lubricating by-products (LBP) and reclaimed rubber powder (RR) to modify asphalt by a one-pot approach, so as to achieve the dual purpose of solving the poor storage stability of reclaimed rubber powder modified asphalt (RRMA) and the realization of solid waste recycling. A variety of characterization techniques were performed to analyze storage stability, conventional properties and microstructure of LBP-activated reclaimed rubber powder modified asphalt (Blend). Fourier transform infrared spectroscopy illustrated that not only the chemical composition of LBP was very similar to that of asphalt, but also the activation of LBP improved the compatibility of RR with asphalt and enhanced the storage stability of Blend. Fluorescence spectrum and scanning electron microscopy results indicated that the RR without LBP activation was aggregated and dispersed as blocks in asphalt, while the LBP activated RR was uniformly dispersed in the asphalt phase. The segregation test demonstrated that Blend exhibited outstanding storage stability, in which the softening point difference was within 2.5 °C and the segregation rate was -0.2-0.2. In addition, the conventional properties of Blend have been significantly improved, especially in penetration and ductility. More importantly, the short-term aging results demonstrated that, compared with RRMA, Blend possessed excellent anti-aging performance.

6.
Adv Sci (Weinh) ; 8(19): e2101727, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34382356

RESUMO

Solar vapor generation technology is promising in seawater desalination, sewage purification, and other fields. However, wide application of this technology is still largely confined due to its high cost and difficulties for scalable production. In this study, an ever-floating solar evaporator is fabricated by coating multiwall carbon nanotubes on a bicomponent nonwoven composed of polypropylene/polyethylene core-sheath fibers. This all-fiber structure is highly porous and ultralight, with large specific area (for efficient water evaporation), interconnected channels (for easy vapor escape), and low thermal conductivity (to avoid heat loss). The unique unidirectional water-transfer behavior of the nonwoven enables it to spontaneously pump an adjustable amount of water for interfacial solar heating and a delicate balance between water supply and loss may accelerate the evaporation speed of water. These distinct benefits endow the solar evaporator with excellent evaporation rates of 1.44 kg m-2  h-1 under the simulated irradiation of 1 sun and 12.81 kg m-2  d-1 under natural sunlight. Moreover, the evaporator can be fabricated by using low-cost materials and industrialized methods (overall cost ≈2.4 USD m-2 ), making one believe its practical significance for commercial solar steam evaporation.

7.
Dent Mater J ; 2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34408120

RESUMO

This study aimed to investigate the effects of silicates on the proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) in vitro. HDPCs were cultured in the presence of calcium silicate (CS) extracts, while calcium hydroxide (CH) extracts and culture medium without CH or CS were used as the control groups. The calcium and phosphorus ion concentrations in the CS were similar to those in the control groups, but the concentration of silicon ions in the CS extracts was higher than that in the control groups. HDPCs cultured with CS and CH extracts at dilution of 1/128 proliferated significantly more than those cultured with the control treatments. CS extracts promoted cell migration, enhanced the expression of odontogenic marker genes and conspicuously increased odontogenesis-related protein production and the release of cytokines, suggesting that CS bioactive ceramics possess excellent biocompatibility and bioactivity and have the potential for application as pulp-capping agents.

8.
Ann Transl Med ; 9(14): 1141, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34430582

RESUMO

Background: To evaluate the efficiency of endodontic retreatment using different instruments and observe whether the Nd:YAP laser can assist. Methods: Eighty premolars were selected, of which the root canals were prepared, and were filled with both gutta-percha and AH plus. The teeth were randomly divided into eight groups, respectively using and not using the Nd:YAP laser with the use of H files, Reciproc files, Mtwo R files, and Mtwo R + H files to remove the root-canal filling material. The retreatment time of each sample was recorded, and the amount of residual gutta-percha was calculated by Image J. Results: (I) In the four no-Nd:YAP laser groups, the Mtwo R group and Reciproc group took the least time, and there was no statistical difference between them (P>0.05). The Mtwo R + H file group took longer than the Mtwo R group and Reciproc group (P<0.05). The H file group took the longest time (P<0.05). (II) In the Nd:YAP laser group, the H file group took the longest time (P<0.05), and there were no significant statistical difference between the other three groups (P>0.05). (III) The Nd:YAP laser groups took a longer time than the no-Nd:YAP laser groups (P<0.05). (IV) All of the groups had residues. (V) The residues in the Mtwo R + H files group and the Reciproc group were the least among the four groups (P<0.05), and the residues in the Mtwo R group were the most (P<0.05). (VI) The residues in the Mtwo R and H file groups of the Nd:YAP laser groups were less than those of the no-Nd:YAP laser groups (P<0.05), and the residues in the Reciproc and the Mtwo R + H file groups of the Nd:YAP laser group showed no statistical difference with that of the no-Nd:YAP laser group (P>0.05). Conclusions: None of the experimental methods could altogether remove the root-filling material. The Nd:YAP laser could assist, but the procedure still took a long time and the efficiency of endodontic retreatment was limited. Using the Mtwo R + H or Reciproc file was the most efficient.

9.
Front Oncol ; 11: 603114, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34277392

RESUMO

Objective: This study aims to identify the potential value of flap endonuclease 1 (FEN1) as a diagnostic and prognostic marker for breast cancer (BC). Methods: ELISA was used to measure serum FEN1 levels and ECLIA for CA153 and CEA levels. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic value. Oncomine and UALCAN databases were used to analyze the differences in FEN1 mRNA and protein expressions. Kaplan-Meier Plotter database was then used to assess the prognostic value. Results: Bioinformatics analysis showed that the FEN1 mRNA and protein levels were significantly higher in BC tissues than in normal tissues. FEN1 was detected in culture medium of BC cell lines and serum FEN1 concentrations were significantly increased in BC patients than in cancer-free individuals. Besides, FEN1 exhibited higher diagnostic accuracy (AUC values>0.800) than CA153 and CEA for distinguishing BC patients, especially early BC, from the healthy and benign groups, or individually. Additionally, serum FEN1 levels were significantly associated with the stage (P=0.001) and lymph invasion (P=0.016), and serum FEN1 levels were increased with the development of BC. Furthermore, serum FEN1 levels were significantly decreased in post-operative patients than in pre-operative patients (P=0.016). Based on the Kaplan-Meier Plotter database, the survival analysis indicated that FEN1 overexpression was associated with poor prognoses for overall survival (OS), relapse-free survival (RFS), and distant metastasis-free survival (DMFS) in BC patients. Conclusion: FEN1 might be a novel diagnostic and prognostic marker for BC.

10.
Dalton Trans ; 50(31): 10758-10764, 2021 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-34313287

RESUMO

Emerging Bi2Se3-based anode materials are attracting great interest for lithium storage because of their high theoretical capacity. Although quite attractive, Bi2Se3 still faces the problem of large volume expansion during lithiation/delithiation, leading to poor cycling stability. Herein, a multi-core yolk-shell Bi2Se3@C nanocomposite was designed and synthesized via a solvothermal method followed by heat treatment. The as-prepared yolk-shell nanocomposite consists of two parts: several Bi2Se3 nanospheres (diameter of approximately 100 nm) as a core, and carbon (thickness of approximately 16 nm) as the shell. Owing to its unique structural features, multi-core yolk-shell Bi2Se3@C nanocomposite demonstrates excellent cycling stability with a capacity of 392.2 mA h g-1 at 0.2 A g-1 after 100 cycles for lithium-ion batteries (LIBs). A reversible capacity of 416.9 mA h g-1 can be maintained even at a higher current density of 1 A g-1 after 1200 cycles. The reason for the superior electrochemical performance was further explored through electrochemical kinetic analysis and theoretical calculations. This work provides an effective strategy for the preparation of multi-core yolk-shell anode materials, and also affords a new method by which to prepare high-performance LIBs.

12.
Am J Transl Res ; 13(5): 4780-4787, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34150058

RESUMO

In previous studies, we found that deferoxamine (DFO) improved the migration of dental pulp cells (DPCs). The present study aimed to determine whether the effects of DFO on the migration of DPCs were regulated via hypoxia-inducible factor 1α (HIF-1α). Recombinant adenovirus vectors carrying short hairpin RNA (shRNA) targeting the human HIF-1α gene (pAd-GFP-shRNA-HIF-1α) and green fluorescent protein (GFP) were constructed. The expression of HIF-1α was inhibited by pAd-GFP-shRNA-HIF-1α at messenger RNA and protein levels. The secretion of stromal cell-derived factor 1α (SDF-1α) or vascular endothelial growth factor (VEGF) in DPCs treated with 10 µM DFO was higher than that in the control condition. The migration of DPCs was enhanced by 10 µM DFO. However, the effects of DFO on DPCs were partially reversed by silencing the HIF-1α gene in enzyme-linked immunosorbent assay or migration assay. Cumulatively, we conclude that DFO upregulated the secretion of SDF-1α or VEGF in DPCs and improved the migration of DPCs through HIF-1α.

13.
J Med Chem ; 64(11): 7434-7452, 2021 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-34011155

RESUMO

BCR-ABL kinase inhibition is an effective strategy for the treatment of chronic myeloid leukemia (CML). Herein, we report compound 3a-P1, bearing a difluoro-indene scaffold, as a novel potent pan-inhibitor against BCR-ABL mutants, including the most refractory T315I mutant. As the privileged (S)-isomer compared to its (R)-isomer 3a-P2, 3a-P1 exhibited potent antiproliferative activities against K562 and Ku812 CML cells and BCR-ABL and BCR-ABLT315I BaF3 cells, with IC50 values of 0.4, 0.1, 2.1, and 4.7 nM, respectively. 3a-P1 displayed a good safety profile in a battery of assays, including single-dose toxicity, hERG K+, and genotoxicity. It also showed favorable mice pharmacokinetic properties with a good oral bioavailability (32%), a reasonable half-life (4.61 h), and a high exposure (1386 h·ng/mL). Importantly, 3a-P1 demonstrated a higher potency than ponatinib in a mice xenograft model of BaF3 harboring BCR-ABLT315I. Overall, the results indicate that 3a-P1 is a promising drug candidate for the treatment of CML to overcome the imatinib-resistant T315I BCR-ABL mutation.


Assuntos
Desenho de Fármacos , Proteínas de Fusão bcr-abl/antagonistas & inibidores , Piperazina/química , Inibidores de Proteínas Quinases/química , Administração Oral , Animais , Sítios de Ligação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Proteínas de Fusão bcr-abl/metabolismo , Meia-Vida , Humanos , Imidazóis/química , Imidazóis/farmacologia , Imidazóis/uso terapêutico , Isomerismo , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Camundongos , Camundongos Endogâmicos ICR , Simulação de Acoplamento Molecular , Mutação , Piperazina/metabolismo , Piperazina/farmacologia , Piperazina/uso terapêutico , Inibidores de Proteínas Quinases/metabolismo , Inibidores de Proteínas Quinases/farmacologia , Inibidores de Proteínas Quinases/uso terapêutico , Piridazinas/química , Piridazinas/farmacologia , Piridazinas/uso terapêutico , Relação Estrutura-Atividade
14.
Int Dent J ; 71(3): 197-205, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33678401

RESUMO

Coronavirus disease 2019 (COVID-19) is an infectious disease that emerged at the end of 2019. On 30 January 2020, the World Health Organization (WHO) classified it as a pandemic. To examine the psychological effects on dental care providers in China in the midst of the COVID-19 outbreak and factors closely associated with those effects, we conducted a cross-sectional study online with 4 widely used self-administered questionnaires: the Patient Health Questionnaire-9, the General Anxiety Disorder-7, the Perceived Stress Scale-10, and the Acute Stress Disorder Scale. Univariate and multivariate analyses were performed to evaluate the variables that potentially affected the mental health of emergency dental care providers. As a result, 969 out of 1035 questionnaires were included in the analysis, with 642 respondents reporting more than 1 symptom (66.3%). The symptom of perceived stress was reported by the largest proportion of the respondents (66.2%, n = 641), and anxiety the least (7.1%, n = 69). After adjustment for confounders, it was found that dental practitioners with preexisting physical health conditions were at higher risk of depression (odds ratio [OR], 1.972; 95% CI, 1.128-3.448; P = .017), and perceived stress (odds ratio, 2.397 95% CI, 1.283-4.478; P = .006). Additionally, feelings of fear, helplessness, or terror resulting from the possibility of contracting COVID-19 were significantly associated with the prevalence of all the 4 psychological symptoms observed (P < .05). In the present study, we found that dental care providers suffered psychological depression, stress, anxiety, and posttraumatic stress disorder (PTSD) during COVID-19, which indicates the importance of psychological support at times of major epidemic outbreaks. Chinese Clinical Trial Registry number: ChiCTR2000031538.


Assuntos
COVID-19 , Pandemias , Ansiedade , China/epidemiologia , Estudos Transversais , Assistência Odontológica , Odontólogos , Depressão/epidemiologia , Humanos , Papel Profissional , SARS-CoV-2 , Estresse Psicológico/epidemiologia , Inquéritos e Questionários
15.
Food Chem ; 340: 127907, 2021 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-32882477

RESUMO

As a homologous material of both medicine and food, licorice is a famous traditional Chinese medicine. In the application process, different origins and different extraction methods have an impact on the intrinsic quality of licorice. In order to ensure the intrinsic quality of commercially available licorice products, and to explore the influence of origin and extraction methods on the quality of licorice, we put forward a simple and effective discriminatory method for "chemometrics analysis-based fingerprint establishment". First, fingerprints of licorice ethanol extraction (LEE) and licorice water extraction (LWE) were established. Then, similarity analysis (SA), hierarchical clustering analysis (HCA), principal component analysis (PCA) and other chemometrics methods were used to select qualitative and quantitative markers. Besides, the quantitative determination of 7 compounds of licorice with statistical significance was carried out, which provided accurate and informative data for quality evaluation. Finally, discriminant analysis was used to trace the origin of licorice.


Assuntos
Glycyrrhiza/química , Extratos Vegetais/química , Fracionamento Químico/métodos , China , Cromatografia Líquida de Alta Pressão/métodos , Clima , Análise por Conglomerados , Análise Discriminante , Medicamentos de Ervas Chinesas/análise , Análise de Alimentos/métodos , Análise de Alimentos/estatística & dados numéricos , Espectrometria de Massas/métodos , Extratos Vegetais/análise , Análise de Componente Principal , Solo
16.
Shanghai Kou Qiang Yi Xue ; 29(2): 123-126, 2020 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-32626872

RESUMO

The outbreak of corona virus disease 2019 (COVID-19) has developed rapidly and the situation of prevention and control is severe. During the epidemic period of COVID-19, due to the particularity of diagnosis and treatment of oral diseases, there is great challenges for how to deal with various types of dental emergency. In order to prevent and control the epidemic situation strictly, and to perform a scientific and orderly clinical diagnosis and treatment of dental emergency, this article provided suggestions on personnel management training, procedures and treatment, protection and disinfection of dental emergency during COVID-19 epidemic, and reference for dental institutions and medical staff.


Assuntos
Betacoronavirus , Infecções por Coronavirus , Assistência Odontológica , Serviços Médicos de Emergência , Pandemias , Pneumonia Viral , COVID-19 , Humanos , Controle de Infecções , SARS-CoV-2
17.
Shanghai Kou Qiang Yi Xue ; 29(2): 127-132, 2020 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-32626873

RESUMO

Corona virus disease 2019 (COVID-19) has become a Public Health Emergency of International Concern since its outbreak, and whether COVID-19 can transmit by aerosol remains controversial. The problem of bio-aerosol transmission in the relatively confined dental clinics has aroused wide attention in the field of dentistry. This review provided a most updated summary on the relation between bio-aerosols and dental clinics, which included the microorganisms in bio-aerosols, the bio-aerosol transmission and the sources testing methods, temporal and spatial distribution of dental bio-aerosols and summarized how to reduce the exposure to bio-aerosols in dental clinics.


Assuntos
Infecções por Coronavirus , Clínicas Odontológicas , Pandemias , Pneumonia Viral , Aerossóis , Betacoronavirus , COVID-19 , Humanos , SARS-CoV-2
18.
Shanghai Kou Qiang Yi Xue ; 29(1): 7-12, 2020 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-32524113

RESUMO

PURPOSE: To analyze the effect of recombinant connective tissue growth factor(rCTGF) on proliferation and differentiation of human dental pulp cells(hDPCs). METHODS: Human dental pulp cells were cultured in vitro and treated with rCTGF at different concentrations (0, 1, 10, 100 ng/mL). The proliferation of dental pulp cells was detected by CCK8 assay. The formation of mineralized nodules was determined by alizarin red staining and half-quantitative alizarin Red S assay. qRT-PCR was utilized to detect the expression of odontogenic differentiation related genes DMP-1, DSPP and OC, and the phosphorylation level of ERK1/2 signaling pathway was detected by Western blot. The data were analyzed with SAS 9.3 software package. RESULTS: High concentration of rCTGF(100 ng/mL) could promote proliferation of dental pulp cells. After mineralization induction, 10 g/mL rCTGF had the best effect on promoting the formation of mineralized nodules in dental pulp cells, and calcium ion deposition was the most obvious(P<0.05). The expression of odontogenic differentiation related genes DMP-1 and DSPP was significantly up-regulated(P<0.05). Western blot results showed that hDPCs stimulated by 10 ng/mL rCTGF could increase the expression of p-ERK1/2. CONCLUSIONS: rCTGF may promote the proliferation and differentiation of human dental pulp cells through activating ERK1/2 signaling pathway.


Assuntos
Fator de Crescimento do Tecido Conjuntivo , Polpa Dentária , Fosfatase Alcalina , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Humanos , Odontogênese
19.
Cancer Cell Int ; 20: 249, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32550827

RESUMO

Background: The mortality and morbidity rates of pancreatic adenocarcinoma have been increasing over the past two decades, and an understanding of the mechanisms underlying pancreatic adenocarcinoma progression is urgently needed. The long non-coding RNA ZFAS1 has been demonstrated to be an oncogene in some cancers, but its function and mechanism in pancreatic adenocarcinoma remain unclear. Methods: The ZFAS1 expression level in pancreatic adenocarcinoma was predicted by bioinformatic analysis, and the expression level of ZFAS1 in pancreatic adenocarcinoma tissue samples and cell lines was further detected by quantitative real-time PCR and in situ hybridization. The functions of ZFAS1 in pancreatic adenocarcinoma in vitro and in vivo were investigated by further bioinformatic analysis. Dual-luciferase reporter assays were used to confirm the binding of ZFAS1/miR-3924 and miR-3924/ROCK2, and rescue assays were performed to further investigate the underlying mechanism. Results: ZFAS1 overexpression in pancreatic adenocarcinoma was predicted and experimentally verified. ZFAS1 silencing inhibited pancreatic adenocarcinoma metastasis in vitro and in vivo. The competing endogenous RNA mechanism of ZFAS1 was also identified. Conclusions: Our results demonstrated the promotive effect of ZFAS1 on pancreatic adenocarcinoma metastasis and suggested its potential role as a novel regulator of ROCK2.

20.
BMC Microbiol ; 20(1): 99, 2020 04 17.
Artigo em Inglês | MEDLINE | ID: mdl-32303183

RESUMO

BACKGROUND: Streptococcus mutans is the principal etiological agent of human dental caries. The major virulence factors of S. mutans are acid production, acid tolerance, extracellular polysaccharide (EPS) synthesis and biofilm formation. The aim of this study is to evaluate the effect of resveratrol, a natural compound, on virulence properties of S. mutans. RESULTS: Resveratrol at sub-MIC levels significantly decreased acid production and acid tolerance, inhibited synthesis of water-soluble polysaccharide and water-insoluble polysaccharide, compromised biofilm formation. Related virulence gene expression (ldh, relA, gtfC, comDE) was down-regulated with increasing concentrations of resveratrol. CONCLUSIONS: Resveratrol has an inhibitory effect on S. mutans cariogenic virulence properties and it represents a promising anticariogenic agent.


Assuntos
Resveratrol/farmacologia , Streptococcus mutans/patogenicidade , Virulência/efeitos dos fármacos , Ácidos/metabolismo , Proteínas de Bactérias/genética , Biofilmes/efeitos dos fármacos , Relação Dose-Resposta a Droga , Regulação para Baixo , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Polissacarídeos Bacterianos/metabolismo , Streptococcus mutans/efeitos dos fármacos , Streptococcus mutans/genética , Streptococcus mutans/metabolismo
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