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1.
Psychiatry Res ; 284: 112690, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31757642

RESUMO

OBJECTIVE: We aimed to study the association among venlafaxine antidepressive outcome, NR3C2 gene polymorphisms and the change of two neuroendocrine hormones during treatment. METHODS: 195 Chinese Han major depressive disorder (MDD) patients were recruited and received a 6-week venlafaxine treatment in this study. Adrenocorticotropic hormone (ACTH), thyroid-stimulating hormone (TSH) levels were measured at the beginning and at the end of treatment. Six single-nucleotide polymorphisms (SNPs) (NR3C2: rs1512325, rs1512342, rs2070951; NR3C1: rs6191, rs6196, rs10482614) were selected for high-throughput SNP genotyping. Allele and genotype frequencies of them were compared between remission and non-remission groups. RESULTS: We found that genotype frequency of the rs1512325 located in the NR3C2 gene was significantly different between remission and non-remission groups respectively (p < 0.05). Meanwhile, the frequency of the rs1512325 C-allele was significantly lower (p < 0.05) in remission group. The TSH concentration significantly increased after venlafaxine treatment in remission group (p < 0.05). CONCLUSION: The rs1512325 in NR3C2 gene and TSH concentration may be related to venlafaxine treatment outcome in Chinese Han MDD patients.

2.
Angew Chem Int Ed Engl ; 59(11): 4401-4405, 2020 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-31849167

RESUMO

Commercial ultrafiltration membranes have proliferated globally for water treatment. However, their pore sizes are too large to sieve gases. Conjugated microporous polymers (CMPs) feature well-developed microporosity yet are difficult to be fabricated into membranes. Herein, we report a strategy to prepare molecular-sieving membranes by partitioning the mesoscopic channels in water ultrafiltration membrane (PSU) into ultra-micropores by space-confined polymerization of multi-functionalized rigid building units. Nine CMP@PSU membranes were obtained, and their separation performance for H2 /CO2 , H2 /N2 , and H2 /CH4 pairs surpass the Robeson upper bound and rival against the best of those reported membranes. Furthermore, highly crosslinked skeletons inside the channels result in the structural robustness and transfer into the excellent aging resistance of the CMP@PSU. This strategy may shed light on the design and fabrication of high-performance polymeric gas separation membranes.

3.
J Biomed Mater Res B Appl Biomater ; 108(1): 272-281, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31013400

RESUMO

Modifications of sintering temperature and treatment time of bovine-derived bone grafts affect their physicochemical properties and further influence biological activity. Three different temperature sintered bovine-derived bone grafts: group I (300 °C 3 h), group II (300 °C 3 h plus 530 °C 6 h), and group III (300 °C 3 h plus 1000 °C 2 h) and Bio-Oss® were characterized and then compared in vitro for their effects on bone marrow stromal cells (BMSCs) migration, proliferation, and differentiation as estimated by cell migration assay, Alkaline phosphatase (ALP) activity assay, and Alizarin red staining. Further, the four bone grafts were implanted into the calvarial defects of rabbits to evaluate bone regeneration and graft degradation. The four deproteinized bovine-derived bone grafts displayed different surface topography. Group II displayed the highest potential of attracting cells. Both groups I and II markedly promote BMSCs differentiation. After 6 and 12 weeks, defects grafted with groups I and II displayed a significant higher bone fraction than defects grafted with group III and Bio-Oss®. Bone graft remnants remained in all four groups. Taken together, sintering at 300 °C for 3 h and sintering at 300 °C for 3 h with an addition of 530 °C for 6 h of bovine-dervied bone grafts displayed potential use in bone regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:272-281, 2020.

4.
Eur J Pharmacol ; 858: 172496, 2019 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-31242440

RESUMO

A natural monoterpene alkaloid incarvillateine isolated from the plant Incarvillea sinensis is known to relieve inflammatory and neuropathic pain. However, the molecular target for the action of incarvillateine remains elusive. Here, we report that incarvillateine exacerbates epileptic seizures by inhibiting subtypes of γ-Aminobutyric acid type A (GABAA) receptors. Two-electrode voltage clamp recordings of α1ß3γ2, α2ß3γ2, α3ß3γ2 and α5ß3γ2 subtypes expressed in Xenopus oocytes revealed that incarvillateine inhibited the GABAA currents with IC50 of 25.1 µM, 43.1 µM, 105.1 µM and 93.7 µM, respectively. Whole-cell patch clamp recordings of hippocampal slices confirmed that incarvillateine inhibited spontaneous inhibitory postsynaptic currents (IPSCs), and miniature IPSCs and tonic currents. Moreover, inhibition of GABAA currents and spontaneous IPSCs by incarvillateine persisted even in the presence of blockers of adenosine receptors. In addition, incarvillateine enhanced epileptic discharges induced by Mg2+-free artificial cerebrospinal fluid (ACSF) in hippocampal slices. Furthermore, intracerebral ventricular injections of incarvillateine increased the severity of seizures induced by kainic acid in a dose-dependent manner. Taken together, our data demonstrate that incarvillateine aggravates seizures by inhibition of GABAA currents and GABAergic synaptic transmissions.


Assuntos
Alcaloides/efeitos adversos , Produtos Biológicos/efeitos adversos , Fenômenos Eletrofisiológicos/efeitos dos fármacos , Antagonistas de Receptores de GABA-A/efeitos adversos , Monoterpenos/efeitos adversos , Receptores de GABA-A/metabolismo , Segurança , Convulsões/fisiopatologia , Animais , Relação Dose-Resposta a Droga , Regulação da Expressão Gênica/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Hipocampo/fisiopatologia , Camundongos , Neurotransmissores/metabolismo , Ratos , Convulsões/induzido quimicamente , Convulsões/metabolismo , Transmissão Sináptica/efeitos dos fármacos
5.
Medicine (Baltimore) ; 98(19): e15456, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31083176

RESUMO

Venlafaxine is one of commonly prescribed antidepressants for major depressive disorder (MDD). Accumulated evidence implicates the involvement of glutamatergic receptors in the pathophysiology of MDD and antidepressant treatment.By using 193 MDD patients who have been taking venlafaxine for 6 weeks, we investigated whether single nucleotide polymorphisms (SNPs) in glutamate ionotropic receptor kainate type subunit 4 (GRIK4), glutamate ionotropic receptor AMPA type subunit 1 (GRIA1) and glutamate metabotropic receptor 7 (GRM7) were associated with treatment response. 14 SNPs were selected randomly depended on association studies. Efficacy of treatment was determined by 17-item of Hamilton Rating Scale. Allele and genotype frequencies were compared between responders and non-responders.After adjusting by the false discovery rate (FDR), rs6589847 and rs56275759 in GRIK4 and rs9870680 in GRM7 showed associating with venlafaxine treatment response at week 6. (FDR: P = .018, P = .042, and P = .040, respectively).Our results indicated that genetic variants in the GRIK4 and GRM7 may associate with the treatment response in MDD patients treated by venlafaxine.


Assuntos
Antidepressivos de Segunda Geração/uso terapêutico , Transtorno Depressivo Maior/tratamento farmacológico , Transtorno Depressivo Maior/genética , Receptores de Ácido Caínico/genética , Receptores de Glutamato Metabotrópico/genética , Cloridrato de Venlafaxina/uso terapêutico , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Feminino , Frequência do Gene , Estudos de Associação Genética , Humanos , Masculino , Variantes Farmacogenômicos , Polimorfismo de Nucleotídeo Único , Receptores de AMPA/genética , Resultado do Tratamento
6.
Clin Neuropharmacol ; 42(2): 32-36, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30875344

RESUMO

Major depressive disorder (MDD) is a common mental disorder. Venlafaxine (VEN) is used to treat patients with MDD as an antidepressant of serotonin-norepinephrine reuptake inhibitor. In addition, current reports reveal that CYP enzymes mediate its metabolism, thereby affecting the treatment efficacy. The aim of this study was to test whether the genetic polymorphisms of CYP1A2 are associated with remission after VEN treatment for MDD. A total of 175 Han Chinese depressed patients have been recruited to accept a 6-week treatment with VEN. Three single-nucleotide polymorphisms of CYP1A2 were selected from dbSNP and previous literature to compare the allele and genotype frequencies between remitters and nonremitters. The A 17-item Hamilton Depression Scale was used to access the improvement of patients' depressive symptoms from the baseline to endpoint. A logistic regression analysis for remission was conducted. Between remitters and nonremitters, the allele and genotype frequencies of single-nucleotide polymorphism rs2470890 demonstrated significant differences. They still had significant differences between remitters and nonremitters after controlling baseline Hamilton Depression Scale scores, sex, and age in logistic regression. Our results suggest that the single-nucleotide polymorphism rs2470890 of CYP1A2 gene might be associated with treatment remission after VEN treatment in patients with MDD.


Assuntos
Antidepressivos de Segunda Geração/uso terapêutico , Grupo com Ancestrais do Continente Asiático/genética , Citocromo P-450 CYP1A2/genética , Transtorno Depressivo Maior/genética , Polimorfismo de Nucleotídeo Único/genética , Cloridrato de Venlafaxina/uso terapêutico , Adulto , Transtorno Depressivo Maior/tratamento farmacológico , Transtorno Depressivo Maior/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Indução de Remissão/métodos , Resultado do Tratamento , Adulto Jovem
7.
Sensors (Basel) ; 19(1)2019 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-30621207

RESUMO

In order to realize automation of the pollutant emission tests of vehicles, a pedal robot is designed instead of a human-driven vehicle. Sometimes, the actual time-speed curve of the vehicle will deviate from the upper or lower limit of the worldwide light-duty test cycle (WLTC) target curve, which will cause a fault. In this paper, a new fault diagnosis method is proposed and applied to the pedal robot. Since principal component analysis (PCA), t-distributed stochastic neighbor embedding (t-SNE), and Autoencoder cannot extract feature information adequately when they are used alone, three types of feature components extracted by PCA, t-SNE, and Autoencoder are fused to form a nine-dimensional feature set. Then, the feature set is reduced into three-dimensional space via Treelet Transform. Finally, the fault samples are classified by Gaussian process classifier. Compared with the methods using only one algorithm to extract features, the proposed method has the minimum standard deviation, 0.0078, and almost the maximum accuracy, 98.17%. The accuracy of the proposed method is only 0.24% lower than that without Treelet Transform, but the processing time is 6.73% less than that without Treelet Transform. These indicate that the multi-features fusion model and Treelet Transform method is quite effective. Therefore, the proposed method is quite helpful for fault diagnosis of the pedal robot.

9.
Psychiatr Genet ; 28(4): 73-74, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29794561

RESUMO

The distal long arm of chromosome 22 (22q13.3) may harbor genes implicated in schizophrenia. This is evidenced by various genetic mapping studies. BRD1 and its neighboring gene ZBED4, both located within this region, have repeatedly been found to be associated with schizophrenia in the Caucasian population. In this study, we chose seven SNPs (two BRD1 SNPs, five ZBED4 SNPs) to carry out an association study between these two genes and schizophrenia in the Chinese population. However, no significant result was obtained, which was consistent with the Japanese population. Taken together, we could conclude that BRD1 and ZBED4 might be population specific in schizophrenia and may not account for a substantial proportion of genetic risk for schizophrenia in the Asian population.


Assuntos
Proteínas de Ligação a DNA/genética , Proteínas Nucleares/genética , Polimorfismo de Nucleotídeo Único , Esquizofrenia/genética , Fatores de Transcrição/genética , Adulto , Alelos , Grupo com Ancestrais do Continente Asiático/genética , Estudos de Casos e Controles , China , Cromossomos Humanos Par 22 , Feminino , Predisposição Genética para Doença , Haplótipos , Chaperonas de Histonas , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem
11.
Zhejiang Da Xue Xue Bao Yi Xue Ban ; 45(1): 86-90, 97, 2016 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-27045247

RESUMO

Sjögren's syndrome is a kind of autoimmune disease, whose main clinical symptoms are dry mouth, dry eye and chronic parotid glandular inflammation. The conservative treatments include artificial tears or saliva,oral administration of corticosteroids,and immunosuppressantsl with limited effectiveness. Along with the development of molecular biology, vast attentions are being paid to researches on gene therapy for Sjögren's syndrome, hopefully to bring gospel to patients with Sjögren's syndrome. This article reviews the recent research progresses on transcatheter delivery of recombinant adenovirus vector with aquaporin gene in experimental treatment of Sjögren's syndrome.


Assuntos
Aquaporinas/genética , Doenças Autoimunes/terapia , Terapia Genética , Síndrome de Sjogren/terapia , Adenoviridae , Cateteres , Vetores Genéticos/administração & dosagem , Humanos
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