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1.
Adv Mater ; : e2000496, 2020 Apr 02.
Artigo em Inglês | MEDLINE | ID: mdl-32239556

RESUMO

High-energy Li-rich layered cathode materials (≈900 Wh kg-1 ) suffer from severe capacity and voltage decay during cycling, which is associated with layered-to-spinel phase transition and oxygen redox reaction. Current efforts mainly focus on surface modification to suppress this unwanted structural transformation. However, the true challenge probably originates from the continuous oxygen release upon charging. Here, the usage of dielectric polarization in surface coating to suppress the oxygen evolution of Li-rich material is reported, using Mg2 TiO4 as a proof-of-concept material. The creation of a reverse electric field in surface layers effectively restrains the outward migration of bulk oxygen anions. Meanwhile, high oxygen-affinity elements of Mg and Ti well stabilize the surface oxygen of Li-rich material via enhancing the energy barrier for oxygen release reaction, verified by density functional theory simulation. Benefited from these, the modified Li-rich electrode exhibits an impressive cyclability with a high capacity retention of ≈81% even after 700 cycles at 2 C (≈0.5 A g-1 ), far superior to ≈44% of the unmodified counterpart. In addition, Mg2 TiO4 coating greatly mitigates the voltage decay of Li-rich material with the degradation rate reduced by ≈65%. This work proposes new insights into manipulating surface chemistry of electrode materials to control oxygen activity for high-energy-density rechargeable batteries.

2.
Lab Chip ; 20(7): 1249-1258, 2020 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-32129401

RESUMO

We propose a coaxial oblique interface shearing (COIS) process for one-step generation of double emulsions which are synchronously sorted with spatial gradient distributions. As a coaxial needle supplying the inner and outer liquids obliquely vibrates across an air-liquid interface, the pinch-off of the compound liquid neck arises and the resultant double emulsions moves with tunable lateral displacements in the receiving phase. In the COIS process, the morphology and size of the double emulsions are heavily dependent on the vibration frequency and the inner and outer liquid flow rates. The lateral droplet displacements changing with process parameters can be precisely controlled in experiments and predicted theoretically by the Stokes drift model. Furthermore, the feasibility of the COIS process in spatial gradient drug release is verified. The double emulsions sorted along a specific direction are available for spatial gradient release under thermal and chemical environments, respectively. The COIS technique has great potential in fields of sensors, spatial gradient materials, advanced drug delivery and biomedical applications.

3.
Med Sci Monit ; 26: e921088, 2020 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-32063600

RESUMO

BACKGROUND Inositol is an essential nutrient for cell growth, survival and embryonic development. Myo-inositol is the predominant form in natural. To investigate the correlation between inositol metabolism and embryonic development, we assessed the metabolic characteristics of myo-inositol, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) of pregnant women in the North China (Yangquan and Weihai) and South China (Nanchang and Haikou) China. MATERIAL AND METHODS All data were collected by face-to-face interview during pregnant women health visits using a questionnaire. Plasma levels of myo-inositol, PI(4,5)P2 and PI(3,4,5)P3 from 89 randomly collected pregnant women were detected by gas chromatography-mass spectrometry and enzyme linked immunosorbent assay. RESULTS A total of 400 pregnant women were included in this survey. The plasma levels of myo-inositol and PI(4,5)P2 in the North China group of pregnant women were significantly higher than that in the South China group (P<0.01). The birth weight of fetuses in the North China group was heavier than that in the South China group (P<0.01). The birth length of fetuses in Yangquan was the longest among the 4 cities (P<0.01). The incidence rate of birth defects was 3.05% in the North China group, and 0.0% in the South China group. In bivariate linear correlation analysis, the body weight correlated with myo-inositol (r=0.5044, P<0.0001), PI(4,5)P2 (r=0.5950, P<0.0001) and PI(3,4,5)P3 (r=0.4710, P<0.0001), the body length was correlated with PI(4,5)P2 (r=0.3114, P=0.0035) and PI(3,4,5)P3 (r=0.2638, P<0.0130). CONCLUSIONS The plasma levels of myo-inositol and PI(4,5)P2 in pregnant women had significant difference between the North and the South of China, which might be correlated with fetal development and birth defects.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32062776

RESUMO

The consumption of rice contaminated with soil cadmium (Cd) threatens human health. It is essential to ensure the production of rice that meets food quality standards. Therefore, a large-scale field survey was conducted in Zhejiang province, southeastern China, to investigate the relationship between Cd accumulation in rice grains and Cd bioavailability in soil, and thus to establish a model to predict Cd contents in rice grains based on soil properties. For this purpose, a total of 156 paired rice and soil samples were collected. Pearson's correlation analysis revealed that Cd measurements obtained by diffusive gradient in thin films (DGT) had a higher correlation (r = 0.818, p < 0.001) with the Cd in rice grains as compared to the Cd measured by the DTPA, CaCl2, EDTA, and HCl extraction methods, which indicated that the DGT technique was a reliable method for the assessment of Cd bioavailability in soils. In addition, among the four extraction methods, the DTPA-extractable Cd showed the highest correlation with the Cd contents in rice grains. Therefore, we developed two predictive models (modelDGT and modelDTPA) to predict Cd levels in rice grains via Cubist multivariate mixed linear regression, using "soil DGT-measured Cd, pH, and oxide contents of Ca, Si, and Fe" or "soil DTPA-extractable Cd, pH, OM, and oxide contents of Ca and Fe" as explanatory variables, respectively. The overall modelDGT and modelDTPA had R2 values of 0.95 and 0.93, respectively, and relative error values of 0.30 and 0.33, respectively. Simple correlation analysis showed direct and close relationships between the measured Cd in rice grains and the Cd concentrations predicted by the Cubist modelDGT and modelDTPA, with R2 values of 0.979 and 0.922, respectively. Therefore, Cd levels in rice grains could be predicted very well based on the two prediction models, and thus, the two models derived in this study are effective in identifying soils in which the Cd in rice grains will exceed food safety standards, thereby helping to ensure safe rice production.

5.
Aging (Albany NY) ; 12(3): 2333-2346, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-32019904

RESUMO

BACKGROUND: Cholangiocarcinoma (CCA) is a serious malignant tumor. Long non-coding RNA NNT-AS1 (NNT-AS1) takes crucial roles in several tumors. So, we planned to research the roles and underlying mechanism of NNT-AS1 in CCA. RESULTS: NNT-AS1 overexpression was appeared in CCA tissues and cell lines. Proliferation was promoted by NNT-AS1 overexpression in CCLP1 and TFK1 cells. Besides, NNT-AS1 overexpression reduced E-cadherin level and raised levels of N-cadherin, vimentin, Snail and Slug. However, the opposite trend was occurred by NNT-AS1 knockdown. Further, NNT-AS1 overexpression promoted phosphatidylinositol 3 kinase (PI3K)/AKT and extracellular signal-regulated kinase (ERK)1/2 pathways. MiR-203 was sponged by NNT-AS1 and miR-203 mimic reversed the above promoting effects of NNT-AS1. Additionally, insulin-like growth factor type 1 receptor (IGF1R) and zinc finger E-box binding homeobox 1 (ZEB1) were two potential targets of miR-203. CONCLUSION: NNT-AS1 promoted proliferation, EMT and PI3K/AKT and ERK1/2 pathways in CCLP1 and TFK1 cells through down-regulating miR-203. METHODS: CCLP1 and TFK1 cells were co-transfected with pcDNA-NNT-AS1 and miR-203 mimic. Bromodeoxyuridine (BrdU), flow cytometry, quantitative reverse transcription polymerase chain reaction (qRT-PCR) and western blot were employed to detect roles and mechanism of NNT-AS1. Interaction between NNT-AS1 and miR-203 or miR-203 and target genes was examined through luciferase activity experiment.

6.
Eur J Pharmacol ; 872: 172915, 2020 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-31926993

RESUMO

The Calcium-sensing receptor (CaSR) is functionally expressed in the biliary epithelial cells and it has been verified to possess various regulatory functions in several different forms of human cancers. But its pathological role in human intrahepatic cholangiocarcinoma (ICC) development remains obscure. Here, we confirmed that CaSR expression was up-regulated in ICC tumor specimens and cell lines, which was positively correlated with number of tumors, lymph node metastasis and poor prognosis of ICC patients. CaSR activation induced by CaCl2 or Calindol (a selective CaSR agonist) markedly facilitated cell proliferation and migration in ICC cells, while knockdown of CaSR or NPS2143 treatment (a CaSR antagonist) dramatically suppressed the above effects. We also demonstrated that alteration of CaSR activity mediated tumorigenesis and growth of ICC in vivo. Mechanistically, CaSR activation could promote cell cycle progression and induce an upregulation of MMP-2 and MMP-9 expression partly via the simulation of ERK1/2 signaling pathway. And further inhibition of ERK pathway significantly suppressed ICC cell viability and migration capacity. Together, our findings shed novel light on the role of CaSR as an oncogene in ICC progression and indicated that modulation of CaSR might serve as a preventive or therapeutic strategy for ICC.

7.
Surg Endosc ; 2020 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-31993813

RESUMO

BACKGROUND: The aim of this study was to evaluate the short-term outcomes and prognosis of laparoscopy-assisted total gastrectomy (LTG) in elderly patients with gastric cancer. METHODS: The clinical data of 275 patients aged over 65 years undergoing open total gastrectomy (OTG, n = 184) or laparoscopy-assisted total gastrectomy (LTG, n = 91) were reviewed from January 2015 to August 2017 at the First Affiliated Hospital of the University of Science and Technology of China. Short-term outcomes were compared between the two groups, and risk factors for postoperative complications were explored. In addition, the 2-year overall survival (OS) and disease-free survival (DFS) were investigated for both groups. RESULTS: Except for the ASA score (P = 0.01), there was no significant difference regarding patient baselines between the two groups. Patients in the LTG group had a longer operative time (P < 0.001), less intraoperative blood loss (P = 0.004), a shorter time of resumption to a semi-liquid diet (P < 0.001) and a shorter postoperative hospital stay (P = 0.001). The incidence of pulmonary complications was significantly lower in the LTG group than in the OTG group (4.4% vs. 13%, P = 0.026). The number of lymph nodes harvested in the LTG group was higher than that in the OTG group (20.7 ± 7.4 vs. 17.5 ± 6.9, P = 0.001), and the proportion of patients with TNM stage III gastric cancer was higher in the LTG group than in the OTG group (P = 0.035). There was no significant difference in the 2-year OS rate or 2-year DFS rate between the two groups (P = 0.057 and P = 0.344). Sex, age, preoperative comorbidity, intraoperative blood loss, and TNM stage were identified as independent prognostic factors for postoperative survival. CONCLUSION: Comparing with OTG, LTG is feasible and contributes to less surgical trauma and a faster recovery after total gastrectomy. In addition, LTG contributes to a lower risk of postoperative pulmonary complications. Regarding oncological results, LTG is more effective for lymph node dissection and has a comparable long-term prognosis as OTG.

9.
Chemosphere ; 241: 125059, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31606571

RESUMO

The climate-driven flooding poses a challenge for phytoremediation of contaminated soil, and the willow (Salix spp.) is a promising candidate coping with climate change and environmental pollution. In this study, uptake and accumulation of copper (Cu), zinc (Zn) and their bioavailability in the rhizosphere across the Salix clones under flooded versus non-flooded (control) conditions were investigated using a pot experiment. The tested Salix clones grew well without showing any toxic symptoms under non-flooded soil condition; in contrast, the clones showed 100% survival for long-term flooding with the development of hypertrophied lenticels and adventitious roots. There were wide clonal variations in biomass production and accumulation of Cu and Zn under flooded and non-flooded conditions. Flooded treatments dramatically decreased aboveground biomass across the Salix clones to different extents compared to the control. The non-flooded clones exhibited relatively high accumulation capacities of Cu and Zn in aerial parts. However, the flooded clones resulted in more substantial reductions in Cu and Zn accumulation in aerial parts, and most of Cu and Zn were limited in roots. EDTA-extractable Cu and Zn predicted well bioavailability of Cu and Zn to the Salix clones under the current condition. It was concluded that the Salix clones exhibited Cu and Zn phytoextraction traits (non-flooding) or phytostabilization traits (flooding), which provides a valuable insight into phytomanagement of contaminated soils by willows subjected to flooding stress.


Assuntos
Adaptação Fisiológica , Biodegradação Ambiental , Cobre/farmacocinética , Inundações , Salix/metabolismo , Zinco/farmacocinética , Biomassa , Cobre/análise , Raízes de Plantas/química , Salix/crescimento & desenvolvimento , Poluentes do Solo/análise , Poluentes do Solo/farmacocinética , Zinco/análise
10.
Arch Orthop Trauma Surg ; 140(2): 219-229, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31728610

RESUMO

BACKGROUND: At present, the best treatment for primary patellar dislocation (PPD) has not been unified. Moreover, meta-analyses comparing the non-surgical and surgical treatments of PPD are lacking. Thus, we aimed to compare the clinical efficacy of surgical or non-surgical treatment of PPD. METHODS: Randomized controlled studies of surgical and non-surgical treatments of PPD from 1966 to 2018 were retrieved from the following databases: PubMed, EMBASE, Cochrane Library, Wanfang Database, China Knowledge Network, Google Scholar, and Weipu Database. We screened for literature that met the inclusion criteria and extracted useful data for our meta-analysis. RESULTS: Nine studies, involving 492 patients, met the inclusion criteria and were analyzed in this study. The recurrence rate of patellar dislocation in the surgical group was lower than that in the non-surgical group (P = 0.04]). Subgroup analysis according to the follow-up time showed that the Kujala score (P < 0.001) and lower recurrence rate of dislocation (P = 0.05) than the non-surgical group in the short term. Subgroup analysis according to surgical year showed that the surgical group get higher Kujala score (P < 0.001) and lower recurrence rate of dislocation (P = 0.01) than the non-surgical group in recent years. CONCLUSION: Surgical treatment can provide better clinical results in a short period of time, and patients may achieve good results within 10 years owing to the advances in surgical techniques and instruments. Thus, we recommend surgical treatment as the preferred treatment for primary patellar dislocation.

11.
PLoS One ; 14(12): e0225751, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31830062

RESUMO

Information spreading in social networks is affected by many factors. Based on a novel information spreading model with five spreading mechanisms, we analyzed and compared the influence of various factors on information spreading. Through a large number of simulation experiments, we found that: (1) K-shell layers have the greatest impact on information spreading; (2) distance between the two information sources, correlation coefficient between two types of information and social reinforcement also affect the information spreading. The analysis results of these factors will be helpful for us to predict the trend of information spreading and find effective strategies to control information spreading.

12.
Adv Mater ; 31(52): e1906156, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31693266

RESUMO

Oxygen vacancies play crucial roles in defining physical and chemical properties of materials to enhance the performances in electronics, solar cells, catalysis, sensors, and energy conversion and storage. Conventional approaches to incorporate oxygen defects mainly rely on reducing the oxygen partial pressure for the removal of product to change the equilibrium position. However, directly affecting reactants to shift the reaction toward generating oxygen vacancies is lacking and to fill this blank in synthetic methodology is very challenging. Here, a strategy is demonstrated to create oxygen vacancies through making the reaction energetically more favorable via applying interfacial strain on reactants by coating, using TiO2 (B) as a model system. Geometrical phase analysis and density functional theory simulations verify that the formation energy of oxygen vacancies is largely decreased under external strain. Benefiting from these, the obtained oxygen-deficient TiO2 (B) exhibits impressively high level of capacitive charge storage, e.g., ≈53% at 0.5 mV s-1 , far surpassing the ≈31% of the unmodified counterpart. Meanwhile, the modified electrode shows significantly enhanced rate capability delivering a capacity of 112 mAh g-1 at 20 C (≈6.7 A g-1 ), ≈30% higher than air-annealed TiO2 and comparable to vacuum-calcined TiO2 . This work heralds a new paradigm of mechanical manipulation of materials through interfacial control for rational defect engineering.

13.
ACS Appl Mater Interfaces ; 11(43): 40932-40943, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31589392

RESUMO

Stimuli-responsive microcarriers (SRMs) based on multiple emulsions can be widely used in advanced drug delivery, tissue engineering, biosensing, and cell biology. Here a simple and effective compound interface shearing (CIS) method is proposed to one-step produce SRMs for controlled ultrasound (US) activation. In the CIS process, a coaxial needle supplying the core and shell liquids vibrates periodically across a free gas-liquid surface, resulting in the pinch-off of a compound liquid neck for on-demand generation of multiple emulsions. The CIS process is free of confined walls with a pure interface shearing mechanism. Perfectly uniform SRMs with tunable core-shell volume ratios can be produced, following a scaling law of their size as a function of the liquid flow rates and the vibration frequency. US- and magnetic-responsive microcapsules are prepared for magnetic-guided site-targeting delivery, and acid-aided sequential US activation realizes the synergistic delivery of hydrophilic and hydrophobic payloads. It can be concluded that the CIS technique is able to generate multifunctional SRMs at low cost, high uniformity, high flexibility, and effective process control for various fields of potential applications.

14.
Front Pharmacol ; 10: 1023, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31572195

RESUMO

Background: Hepatitis B virus (HBV) coinfection is common in HIV-positive patients. HIV infection modifies the natural course of HBV infection, leading to a faster progression of liver-related morbidity and mortality than is observed in HBV mono-infected patients. This systematic review and meta-analysis evaluates the current clinical evidence regarding the use of oral tenofovir disproxil fumarate (TDF)-based treatments in patients coinfected with HIV and HBV. Methods: We performed a comprehensive literature search in PubMed and Web of Science. Supplementary searches were conducted in Google Scholar and Clinicaltrials.gov. We conducted a random effects meta-analysis using the event rate (ER) to estimate the incidence of HBV seroconversion. A subgroup meta-analysis was performed to assess the moderate effects of demographic and disease-related variables on HBsAg loss. This review is registered in the PROSPERO database (CRD42018092379). Results: We included 11 studies in the review. The immunological effects of oral TDF-based Pre-exposure prophylaxis (PrEP) treatment in patients with HIV-HBV coinfection were 0.249 for HBeAg loss, 0.237 for HBeAg conversion, 0.073 for HBsAg loss, and 0.055 for HBsAg conversion. The factors associated with HBsAg loss were the baseline HBV viral load, participant's location, and a history of exposure to lamivudine/emtricitabine (3TC/FTC) (all p < 0.05). A trend toward a negative relationship between the baseline CD4+ T-cell count and HBsAg loss was observed (p = 0.078). Conclusion: This systematic review and meta-analysis demonstrated that TDF-containing regimens are effective at stimulating HBeAg loss (24.9%), HBeAg conversion (23.7%), HBsAg loss (7.3%), and HBsAg conversion (5.5%) in HIV-HBV coinfected patients. The moderator analysis showed that HBV viral load, the location of participants, and prior exposure to 3TC/FTC are factors associated with HBsAg loss. Asian ethnicity, prior exposure to 3TC, and a nondetectable baseline HBV viral load are associated with lower odds of HBsAg loss. Well-designed prospective cohort studies and randomized controlled trials (RCTs) with large sample sizes are required for the investigation of potential predictors and biological markers associated with strategies for achieving HBV remission in patients with HIV-HBV coinfection, which is a matter of considerable importance to clinicians and those responsible for health policies.

15.
ACS Appl Mater Interfaces ; 11(43): 40320-40331, 2019 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-31603311

RESUMO

A novel host material of "M"-type carbazole/fluorene-based mDCzPF with a high triplet energy by utilizing meta-substituted phenyl groups as linkers was developed. It was demonstrated that the position of the substituents significantly affected the molecular configuration and dipole moment, which played a critical role in the device performances. Red phosphorescent OLED utilizing the "M"-type mDCzPF as the host represented a 10-fold operational lifetime improvement over the OLED using a "V"-type pDCzPF linked by para-substituted phenyl groups as the host because of the good charge transport ability of the mDCzPF. Additionally, the "M"-type mDCzPF host was also compatible with a blue emitting phosphorescent emitter PtNON. The PtNON-doped OLED using mDCzPF as the host exhibited a peak EQE of 18.3% with a small roll off, yet maintained an EQE of 13.3% at a high brightness of 5000 cd/m2. Thus, the novel "M"-type mDCzPF could be employed as stable host material for efficient OLED emitting across the whole visible spectrum. This study should provide a viable method for designing new host materials for the development of stable and efficient phosphorescent OLEDs.

16.
Am J Transl Res ; 11(9): 5438-5456, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31632521

RESUMO

BACKGROUND: Cholangiocarcinoma (CCA) is a biliary malignancy, which is notoriously difficult to diagnose and associated with poor survival. Accumulating evidence indicates that long non-coding RNA Nicotinamide Nucleotide Transhydrogenase-antisense RNA1 (NNT-AS1) is overexpressed in several tumors and plays a crucial role in the development of neoplasm. However, the expression pattern and functional role of NNT-AS1 in CCA remain largely unknown. METHODS: NNT-AS1 expression was assessed by RT-qPCR and In Situ Hybridization (ISH) assay. The clinical relevance of NNT-AS1 was analyzed using a CCA tissue microarray with follow-up data. The function role of NNT-AS1 and its underlying molecular mechanisms were evaluated using both in vitro/in vivo experiments and bioinformatics analysis. Luciferase reporter assay, western blot and RT-qPCR were conducted to identify the miRNA/target gene involved in the regulation of CCA progression. RESULTS: LncRNA NNT-AS1 was found highly expressed in CCA. Upregulated NNT-AS1 expression was tightly associated with clinical malignancies and predicted poor prognosis of CCA patients. Functional studies showed that NNT-AS1 knockdown inhibited cell proliferation, migration and invasion of CCA cells in vitro. Conversely, NNT-AS1 overexpression showed the opposite biological effects. In a tumor xenograft model, we confirmed that NNT-AS1 knockdown could significantly inhibit the growth of CCA, while NNT-AS1 overexpression promoted CCA development. Mechanistically, we demonstrated that NNT-AS1 might function as a ceRNA in regulating HMGA2 (high mobility group AT-hook 2) through competitively binding to miR-142-5p in CCA. Moreover, we showed that NNT-AS1 regulated epithelial-mesenchymal transition in CCA. CONCLUSION: In summary, these findings suggest the potential prognostic and therapeutic value of NNT-AS1/miR-142-5p/HMGA2 axis in CCA patients.

17.
Life Sci ; 235: 116819, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31473194

RESUMO

AIMS: Traumatic brain injury (TBI) not only induces physiological disabilities but also leads to cognitive impairment. However, no effective therapeutic approach for TBI-related memory decline exists. In this study, we treated TBI mice with cinnamic acid (CNA) to detect whether CNA is able to rescue the memory deficits induced by TBI and to explore the potential mechanisms. MAIN METHODS: Mice were divided into the following groups: the sham group, the TBI group, the TBI + CNA group and the CNA group. Basic physiological parameters, neurological severity score and brain water content were analyzed. The Morris water maze and inhibitory avoidance step-down task were used to determine learning and memory. Golgi staining was used to measure alterations in dendritic spines. Western blot analysis and a commercial kit were used to detect the content and activity of HDAC2. qPCR was used to detect the relative level of miR-455. KEY FINDINGS: CNA did not affect physiological function but effectively restored neurological function and brain edema. CNA alleviated the memory impairments induced by TBI in both the Morris water maze and step-down task. CNA also recovered abnormalities in the synapses of TBI mice by suppressing the activity of HDAC2. Furthermore, CNA did not alter HDAC mRNA because it promoted the expression of miR-455-3p, a miRNA that regulates HDAC2 at the posttranscriptional level. SIGNIFICANCE: The application of CNA effectively treats TBI-induced memory deficits by increasing miR-455-3p and by inhibiting HDAC2.


Assuntos
Comportamento Animal/efeitos dos fármacos , Lesões Encefálicas Traumáticas/complicações , Cinamatos/farmacologia , Histona Desacetilase 2/metabolismo , Transtornos da Memória/prevenção & controle , MicroRNAs/genética , Transmissão Sináptica/efeitos dos fármacos , Animais , Histona Desacetilase 2/genética , Masculino , Aprendizagem em Labirinto , Transtornos da Memória/etiologia , Transtornos da Memória/metabolismo , Camundongos , Camundongos Endogâmicos C57BL
18.
J Microencapsul ; 36(7): 649-658, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31490709

RESUMO

Aim: To reduce the contamination arising from abuse of commercial pesticide formulations, the coaxial electrospray (CES) method was used for one-step microencapsulation and spraying of pesticides. Methods: After optimisation of process parameters, polymeric microcapsules with different structures were fabricated as the carriers of azoxystrobin (AZS). For the resultant microcapsules, the sustained pesticide release was verified in vitro and the adhesion properties were investigated through a normalised rinsing test. Results: The maximum encapsulation efficiency of the fabricated AZS-loaded microcapsules was 99.14%. Compared to commercial AZS aqueous suspension, the microcapsules fabricated by the CES method exhibited improved sustained release performance of AZS, which could be readily controlled by adjusting the shell thicknesses. Moreover, highly enhanced adhesion performance was observed for the AZS-loaded microcapsules directly sprayed in CES process. Conclusions: The CES process is promising to be applied as a one-step microencapsulation and spraying technology for improving pesticide utilisation and reducing environmental pollution.

19.
J Am Chem Soc ; 141(36): 14038-14042, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31448603

RESUMO

Sluggish interfacial kinetics leading to considerable loss of energy and power capabilities at subzero temperatures is still a big challenge to overcome for Li-ion batteries operating under extreme environmental conditions. Herein, using LiMn2O4 as the model system, we demonstrated that nickel surface doping to construct a new interface owning lower charge transfer energy barrier, could effectively facilitate the interfacial process and inhibit the capacity loss with decreased temperature. Detailed investigations on the charge transfer process via electrochemical impedance spectroscopy and density functional theory calculation, indicate that the interfacial chemistry tuning could effectively lower the activation energy of charge transfer process by nearly 20%, endowing the cells with ∼75.4% capacity at -30 °C, far surpassing the hardly discharged unmodified counterpart. This control of surface chemistry to tune interfacial dynamics proposes insights and design ideas for batteries to well survive under thermal extremes.

20.
Mater Sci Eng C Mater Biol Appl ; 102: 113-123, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31146981

RESUMO

Ovarian cancer is one of the most lethal gynecologic malignancies due to its rapid proliferation, frequent acquisition of chemoresistance, and widespread metastasis within the peritoneal cavity. Intraperitoneal (IP) chemotherapy has demonstrated significant anti-cancer potential but its broad clinical application is hindered by several drug delivery limitations. Herein, we engineer paclitaxel (PTX) laden hybrid microparticles (PTX-Hyb-MPs) for improved delivery of chemotherapy in ovarian cancer. The PTX-Hyb-MPs are comprised of a lipid-coated shell of poly (lactic acid-co-glycolic acid) (PLGA) encapsulating hydrophobic PTX. A co-axial electrohydrodynamic (CEH) process is used for one-step and scalable production of the PTX-Hyb-MP agent with controlled particles size, uniform size distribution, tunable thickness, and high encapsulation rate (92.17 ±â€¯6.9%). The multi-layered structure of the PTX-Hyb-MPs is verified by transmission electron microscopy and confocal fluorescence microscopy. The effect of lipid coating on the enhancement of particle interactions with cancer cells is studied by flow cytometry and confocal fluorescence microscopy. The anti-cancer effect of the PTX-Hyb-MPs is evaluated in SKOV-3 ovarian cancer cells in vitro and a cancer xenograft model in vivo, in comparison with conventional drug delivery methods. Our studies reveal that the PTX-Hyb-MP agent can be potentially used for locoregional treatment of ovarian cancer and other tissue malignancies with sustained drug release, tunable release profiles, enhanced drug uptake, and reduced systemic toxicity.


Assuntos
Materiais Biocompatíveis/química , Sistemas de Liberação de Medicamentos , Microesferas , Neoplasias Ovarianas/tratamento farmacológico , Paclitaxel/administração & dosagem , Paclitaxel/uso terapêutico , Animais , Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Endocitose/efeitos dos fármacos , Feminino , Humanos , Camundongos Nus , Neoplasias Ovarianas/patologia , Paclitaxel/farmacologia , Tamanho da Partícula
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