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1.
Water Res ; 173: 115596, 2020 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-32062228

RESUMO

Selective reduction of nitrate to N2 is attractive but still a difficult challenge in the water treatment field. Herein, we established a flow-through electrochemical system packed with polymeric beads supported nZVI (nZVI@D201) for selective nitrate reduction. Consequently, efficient nitrate reduction in the flow mode was achieved on nZVI@D201 under electrochemical regulation with N2 selectivity of up to 95% for at least 60 h. Otherwise, nZVI was gradually exhausted after 20 h, and the product was mainly the undesired NH4+. Through a series of comparative experiments, we clarified that the enhanced nitrate reduction on nZVI under electrochemical regulation was mainly attributed to electrons (from cathode) and active hydrogen ([H]) rather than the previously speculated H2. Combining the characterizations of nZVI during nitrate reduction by X-ray diffraction and X-ray photoelectron spectrometry, we found that nitrate reduction under electrochemical regulation was mediated by nZVI along with the resultant Fe0@FexOy-Fe(II) structure and was sustained by electrons (from cathode) and [H] via the in situ reduction of Fe(III) back to Fe(II). Meanwhile, the undesirable product NH4+ was efficiently oxidized to N2 by the active chlorine generated on the anode. This study not only clarifies the mechanism of enhanced nitrate reduction on nZVI via electrochemical regulation but also advances the technological coupling of nZVI reduction with electrochemistry.

2.
Chemosphere ; 240: 124940, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31574446

RESUMO

Charged ultrafiltration (UF) membranes can repel electrically charged molecules that are smaller than the size of the membrane pores and display high rejection of solutes, high flux, and low operation pressures compared to uncharged UF, nanofiltration (NF) and reverse osmosis (RO). Here, a charged UF membrane composite (PANI/PVDF) was prepared and regulated via electrochemically reversible control in portions of amine/imine functional groups of PANI. As a result, the permeability and rejection ratios of CR2- on charged PANI/PVDF, with PVDF as a control, increased from 19.6 to a maximum of 183.3 L m-2 h-1 bar-1 and from 3.4% to 74%, which expands the trade-off confine benefited from surface potential change from -12.21 mV to -25.26 mV, furtherly, the rejection ratio of CR2- on PANI/PVDF reached up to 93% via the electrochemical regulation. Finally, a fixed-charge model was built that well describes the steric and electric repulsion effects on membrane performance and the important roles of the electrochemically controllable surface charge. Moreover, the contour map of rejection ratios containing the ratio of molecular size vs the average pore size of the membrane (r/R = 0.2-1.0) and the zeta potential (-10 to -60 mV) were taken into account, which can be used to visually understand the rejection performance of membranes. This model is also appropriate for varying molecular sizes and for molecules with different charges. Our work opens a new horizon for the design of electrochemically controllable charged membranes to remove charged compounds.


Assuntos
Corantes/análise , Técnicas Eletroquímicas/métodos , Ultrafiltração/métodos , Purificação da Água/métodos , Eletricidade , Membranas Artificiais , Propriedades de Superfície
3.
BMC Nephrol ; 19(1): 285, 2018 10 22.
Artigo em Inglês | MEDLINE | ID: mdl-30348110

RESUMO

Chronic kidney disease (CKD) is an inherently systemic disease that refers to a long-term loss of kidney function. The progression of CKD has repercussions for other organs, leading to many kinds of extrarenal complications. Intensive studies are now being undertaken to reveal the risk factors and pathophysiological mechanism of this disease. During the past 20 years, increasing evidence from clinical and basic studies has indicated that klotho, which was initially known as an anti-aging gene and is mainly expressed in the kidney, is significantly correlated with the development and progression of CKD and its complications. Here, we discuss in detail the role and pathophysiological implications of klotho in ion disorders, the inflammation response, vascular calcification, mineral bone disorders, and renal fibrosis in CKD. Based on the pathogenic mechanism of klotho deficiency and klotho decline in urine early in CKD stage 2 and even earlier in CKD stage 1, it is not difficult to understand that soluble klotho can serve as an early and sensitive marker of CKD. Moreover, the prevention of klotho decline by several mechanisms can attenuate renal injuries, retard CKD progression, ameliorate extrarenal complications, and improve renal function. In this review, we focus on the functions and pathophysiological implications of klotho in CKD and its extrarenal complications as well as its potential applications as a diagnostic and/or prognostic biomarker for CKD and as a novel treatment strategy to improve and decrease the burden of comorbidity in CKD.


Assuntos
Glucuronidase/fisiologia , Insuficiência Renal Crônica/genética , Insuficiência Renal Crônica/metabolismo , Anti-Inflamatórios/uso terapêutico , Biomarcadores/metabolismo , Fatores de Crescimento de Fibroblastos/genética , Fatores de Crescimento de Fibroblastos/metabolismo , Glucuronidase/uso terapêutico , Humanos , Insuficiência Renal Crônica/tratamento farmacológico
4.
J Cell Biochem ; 119(7): 5875-5884, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29575081

RESUMO

Most animals hold the ability to regenerate damaged cells, tissues, and even any lost part of their bodies. To date, there is little known about the precise regulatory mechanism of regeneration and many fundamental questions remain unanswered. To further understand the precise regulatory mechanism of regeneration, we used planarian Dugesia japonica as a model and sequenced the transcriptomes of their regenerated tissues at different regeneration stages. Through de novo assembly and expression profiling, we found that Heat shock protein and MAPK pathway were involved into early response of regeneration in D. japonica. In addition, immune response, cell proliferation, and migration were activated during regeneration. Of notes, our results revealed a specific functional role of programmed cell death (PCD) in regeneration of D. japonica. PCD may not only remove the damaged and superfluous tissues for further patterning with regenerated tissues, but also provide signals to trigger neoblasts proliferation and differentiation directly. Together, our results revealed Heat shock protein and MAPK pathway mediated early response of regeneration and found a dual role of PCD in regeneration D. japonica. Meanwhile, we constructed regulatory networks of apoptosis, autophagy, and related signaling pathways and proposed a schematic model, which provided a global landscape of regeneration.


Assuntos
Apoptose/genética , Perfilação da Expressão Gênica , Proteínas de Helminto/genética , Planárias/genética , Regeneração/genética , Transcriptoma , Animais , Diferenciação Celular , Regulação da Expressão Gênica , Redes Reguladoras de Genes , Planárias/crescimento & desenvolvimento
5.
J Sep Sci ; 40(21): 4102-4112, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28841261

RESUMO

Traditional Chinese medicine is the clinical experience accumulated by Chinese people against diseases. Da-Bu-Yin-Wan is a famous traditional Chinese medicine formula consisting of Phellodendri amurensis Rupr., Anemarrhenae asphodeloides Bge., Radix Rehmanniae Preparata and Chinemys reevesii. In this study, ultra high performance liquid chromatography with electrospray ionization quadrupole time-of-flight high-definition mass spectrometry with the control software of Masslynx (V4.1) was established for comprehensive screening and identification of the chemical constituents and serum metabolites of Da-Bu-Yin-Wan in vivo and in vitro. Consequently, 70 peaks in the methanol extract from Da-Bu-Yin-Wan and 38 peaks absorbed into rat blood were characterized. The 70 constituents in vitro included alkaloids, flavonoids, polysaccharide, limonoids, flavonoid, etc. And the 38 constituents consist of 22 absorbed prototypes and 16 metabolites of Da-Bu-Yin-Wan absorbed in vivo. We fully clarified the chemical constituents of Da-Bu-Yin-Wan and provided a scientific strategy for the screening and characterization of the chemical constituents and metabolites of traditional Chinese medicine in vitro and in vivo.


Assuntos
Medicamentos de Ervas Chinesas/química , Compostos Fitoquímicos/análise , Animais , Cromatografia Líquida de Alta Pressão , Extratos Vegetais/química , Ratos , Espectrometria de Massas por Ionização por Electrospray
6.
Oncol Rep ; 37(3): 1461-1468, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28184944

RESUMO

To explore the complex molecular mechanisms of bladder cancer, mRNA and miRNA expression profiles were combined for systematic analyses. A total of 18 common differentially expressed genes (DEGs) were identified from two mRNA expression datasets which consisted of 206 tumor and 74 normal tissues. Then, survival analysis based on the SurvExpress database showed that the common DEGs were able to significantly differentiate low- and high-risk groups in 4 public bladder cancer datasets (p<0.01). Notably, the tumor and normal samples were able to be almost clearly classified into 4 groups based on these identified common DEGs. In addition, 6 out of the 18 common DEGs, including ALDH1A1 and SRPX, are regulated by 6 reported miRNAs based on regulatory network analyses. Expression levels of the 6 DEGs were validated in 10 bladder cancer samples using RT-PCR, and the expression values were concordant with the microarray results. Collectively, our analyses indicated that various biological processes are involved in the development and progression of bladder cancer. Firstly, cell cycle checkpoints and DNA repair networks of cancer stem-like cells were regulated by high expression of ALDH1A1, and hence promoted tumor self-renewal or metastasis. Then, activation of HspB6 induced the angiogenesis process which provides necessary nutrition and oxygen for tumor cells. Moreover, downregulation of the expression of tumor-suppressor genes SRPX and FLNC further promoted apoptosis and metastasis. The identification of potential biological processes and genes can be helpful for the understanding of bladder cancer molecular mechanisms.


Assuntos
Aldeído Desidrogenase/genética , Regulação Neoplásica da Expressão Gênica , Redes Reguladoras de Genes , MicroRNAs/genética , RNA Mensageiro/genética , Neoplasias da Bexiga Urinária/genética , Perfilação da Expressão Gênica , Humanos , Reação em Cadeia da Polimerase em Tempo Real , Retinal Desidrogenase , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Neoplasias da Bexiga Urinária/patologia
7.
J AAPOS ; 21(1): 15-18, 2017 02.
Artigo em Inglês | MEDLINE | ID: mdl-28089744

RESUMO

BACKGROUND: In patients with moderate- to large-angle exotropic deviations, surgical correction remains the definitive treatment. The purpose of this study was to identify preoperative factors that correlate with surgical success and to produce a binomial model that predicts success based on preoperative factors. METHODS: We performed a retrospective review of patients with intermittent exotropia who underwent uniocular recession-resection surgery. Success was defined as ocular deviation ≤10Δ exotropia and ≤5Δ esotropia at distance at last follow-up (at least 3 months' postoperatively). Preoperative factors such as age at surgery, sex, visual acuity, spherical equivalent, prism fusion range, stereopsis, and ocular deviation were analyzed for correlation with success using binomial logistic regression. RESULTS: A total of 82 patients were identified (average age, 33 years; range, 3.7-81.6 years). Average prismatic deviation was 35Δ at near and 34Δ at distance. Average stereopsis was 167″. The average success rate was 58.5%. Univariate binomial regression revealed that patients with smaller angle of deviation at near (OR = 0.96, P = 0.013) or distance (OR = 0.96, P = 0.005), larger myopic refractive errors in terms of mean spherical equivalent (OR = 0.71, P = 0.022) and spherical equivalent in the more myopic eye (OR = 0.75, P = 0.029) contributed to success. A multivariate regression model was able to predict success with an accuracy of 72% (sensitivity, 81%; specificity, 58%; negative predictive value, 67%; positive predictive value, 74%). CONCLUSIONS: A smaller preoperative angle of deviation and larger myopic refractive error correlated with success in uniocular intermittent exotropia surgery in our cohort. Larger deviations and more hyperopia correlated with lower success rates.


Assuntos
Exotropia/cirurgia , Músculos Oculomotores/cirurgia , Procedimentos Cirúrgicos Oftalmológicos , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Percepção de Profundidade/fisiologia , Exotropia/fisiopatologia , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Músculos Oculomotores/fisiopatologia , Prognóstico , Estudos Retrospectivos , Visão Binocular/fisiologia , Acuidade Visual/fisiologia
8.
Oncol Res ; 25(1): 29-34, 2017 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-28081730

RESUMO

Aberrantly expressed microRNAs (miRNAs/miRs) and their role in cancer development have recently gained more attention. However, the potential role of miRNAs in hepatocellular carcinoma (HCC) remains largely unknown. In this study, we demonstrated that miR-377 was markedly downregulated in HCC cell lines and primary human HCC tissues. The decreased expression of miR-377 contributes to the upregulation of Bcl-xL expression by targeting its 3'-untranslated region (3'-UTR). Functionally, knockdown of miR-377 noticeably increased HCC cell growth and colony formation and inhibited apoptosis. In contrast, overexpression of miR-377 suppressed cell proliferation and increased apoptosis. This study provides new insights for the use of miR-377 as a potential molecular target in HCC therapy.


Assuntos
Apoptose/genética , Carcinoma Hepatocelular/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/genética , MicroRNAs/genética , Interferência de RNA , Proteína bcl-X/genética , Regiões 3' não Traduzidas , Sequência de Bases , Sítios de Ligação , Linhagem Celular Tumoral , Proliferação de Células , Regulação para Baixo , Humanos
9.
Oncol Res ; 23(6): 321-326, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-27931288

RESUMO

Synthetic CpG oligodeoxynucleotides (ODNs), as TLR9 agonists, have been found to play a possible role in antitumor effect. In order to determine the effect of YW002, known as a C-type CpG ODN, on the treatment of hepatocellular carcinoma (HCC), which is one of the most aggressive carcinomas, we chose to inject YW002 at the doses of 12.5 g and 25 g per mouse 7 days post-tumor challenge. The survival rate of mice was recorded every day. On day 14 postinjection, five mice in each group were bled and randomly sacrificed. The level of IFN- or TNF- in the serum was detected and lymphocyte infiltration in the tumor tissue; the ratios of CD8+ T cells and CD4+ T cells in the spleen of mice were also analyzed. The results indicated that treatment with YW002 could raise the survival rate and delay tumor growth in the mice with orthotopically transplanted HCC. Furthermore, the treatment improved the antitumor immune response through increasing the T-cell infiltration in tumor and the ratio of CD4+, CD8+, and NK cells in the spleen. In addition, the concentration of IFN- was raised, and the level of TGF- was depressed. Our data suggested that CpG ODN might be a proper medicament in a monotherapeutic regimen for treatment of HCC.


Assuntos
Carcinoma Hepatocelular/imunologia , Carcinoma Hepatocelular/terapia , Neoplasias Hepáticas/imunologia , Neoplasias Hepáticas/terapia , Oligodesoxirribonucleotídeos/administração & dosagem , Animais , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/patologia , Linfócitos T CD8-Positivos/imunologia , Linfócitos T CD8-Positivos/patologia , Carcinoma Hepatocelular/genética , Linhagem Celular Tumoral , Humanos , Interferon gama/sangue , Neoplasias Hepáticas/genética , Linfócitos do Interstício Tumoral/imunologia , Linfócitos do Interstício Tumoral/patologia , Camundongos , Camundongos Endogâmicos C57BL , Fator de Necrose Tumoral alfa/sangue , Ensaios Antitumorais Modelo de Xenoenxerto
10.
J Sep Sci ; 39(24): 4700-4711, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27778479

RESUMO

Zi Shen Wan is a typical formula consisting of three herbs, Phellodendri Amurensis Cortex, Rhizoma Anemarrhenae, and Cortex Cinnamomi, and has been widely used for treating prostatitis and infection diseases. However, it lacks in-depth research of the constituents of Zi Shen Wan in vivo and in vitro. In this work, ultra high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and MassLynx software was established to characterize the chemical compositions of Zi Shen Wan in vivo and in vitro. In total, 92 peaks were characterized in vitro and 33 peaks were characterized in vivo based on mass spectrometry and tandem mass spectrometry data. Among the 33 compounds characterized in rat plasma, 22 prototype components absorbed in rat serum and 11 metabolites were identified in vivo. This work was fully reports the chemical constituents of traditional Chinese formula of Zi Shen Wan, it demonstrated that ultra high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry coupled to MassLynx software and multivariate data processing approach could be successfully applied for rapid screening and comprehensive analysis of chemical constituents in vitro and prototype components or metabolites in vivo of traditional Chinese medicine.


Assuntos
Medicamentos de Ervas Chinesas/química , Compostos Fitoquímicos/sangue , Administração Oral , Animais , Cromatografia Líquida de Alta Pressão , Masculino , Análise Multivariada , Compostos Fitoquímicos/farmacocinética , Ratos , Espectrometria de Massas em Tandem
11.
BMC Cardiovasc Disord ; 16(1): 183, 2016 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-27663794

RESUMO

BACKGROUND: The prevalence of hypertension in adults is increasing each year and has become a main public health issue worldwide. We must consider the impact of both individual factors and interactions among these factors on hypertension in adults. This study was designed to elucidate the clinical and metabolic characteristics of the prevalence of hypertension in adults and to explore the risk factors and interactions among these factors in adults with hypertension. METHODS: We used overall random sampling to conduct a cross-sectional survey of 6660 individuals undergoing a health check from July to November 2012, the subjects were aged 20 to 89 years, including 3480 men and 3180 women. The survey content included a questionnaire, anthropometry, laboratory measurements, and liver Doppler ultrasonography. The clinical and metabolic characteristics were compared between the cases (adult hypertensive patients) and the controls (normotensives). The classification tree model and the non-conditional logistic regression were used to analyze the interactions of risk factors for hypertension in adults. RESULTS: In total, 1623 adult hypertensive patients (940 men and 683 women) were detected. The results showed that adult hypertensive patients were older and had higher levels of systolic blood pressure, diastolic blood pressure, body mass index, fasting plasma glucose, uric acid, triglycerides, total cholesterol, low-density lipoprotein cholesterol, and prevalence of non-alcoholic fatty liver disease (P < 0.001). The classification tree model comprising 5 layers, 39 nodes, and 20 terminal nodes showed that two variables, age and BMI, were closely related to hypertension in adults. The area under the receiver operating characteristic curve for classification tree model was 81.6 % (95 % CI: 80.6 % ~ 82.5 %). Both univariate and multivariate logistic regression analyses revealed that advanced age and high BMI had a significant positive interaction in terms of hypertension in adults. After controlling for confounding factors, the percentage of attributed interaction was 47.62 %. CONCLUSIONS: This study showed that age, BMI, UA, TG, and TC were closely associated with the risk of hypertension in adults, and the positive interaction effect between advanced age and high BMI was an important risk factor for the prevalence of hypertension in adults.

12.
Oncol Res ; 23(6): 321-6, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27131318

RESUMO

Synthetic CpG oligodeoxynucleotides (ODNs), as TLR9 agonists, have been found to play a possible role in antitumor effect. In order to determine the effect of YW002, known as a C-type CpG ODN, on the treatment of hepatocellular carcinoma (HCC), which is one of the most aggressive carcinomas, we chose to inject YW002 at the doses of 12.5 µg and 25 µg per mouse 7 days post-tumor challenge. The survival rate of mice was recorded every day. On day 14 postinjection, five mice in each group were bled and randomly sacrificed. The level of IFN-γ or TNF-α in the serum was detected and lymphocyte infiltration in the tumor tissue; the ratios of CD8(+) T cells and CD4(+) T cells in the spleen of mice were also analyzed. The results indicated that treatment with YW002 could raise the survival rate and delay tumor growth in the mice with orthotopically transplanted HCC. Furthermore, the treatment improved the antitumor immune response through increasing the T-cell infiltration in tumor and the ratio of CD4(+), CD8(+), and NK cells in the spleen. In addition, the concentration of IFN-γ was raised, and the level of TGF-ß was depressed. Our data suggested that CpG ODN might be a proper medicament in a monotherapeutic regimen for treatment of HCC.


Assuntos
Adjuvantes Imunológicos , Carcinoma Hepatocelular/imunologia , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/imunologia , Neoplasias Hepáticas/patologia , Oligodesoxirribonucleotídeos/administração & dosagem , Oligodesoxirribonucleotídeos/imunologia , Animais , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/mortalidade , Linhagem Celular Tumoral , Citocinas/sangue , Citocinas/metabolismo , Modelos Animais de Doenças , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/mortalidade , Linfócitos do Interstício Tumoral/imunologia , Linfócitos do Interstício Tumoral/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Baço/imunologia , Baço/metabolismo , Subpopulações de Linfócitos T/imunologia , Subpopulações de Linfócitos T/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto
13.
Onco Targets Ther ; 9: 2717-23, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27217782

RESUMO

AIM: This study aimed to select several signature genes associated with bladder cancer, thus to investigate the possible mechanism in bladder cancer. METHODS: The mRNA expression profile data of GSE31614, including ten bladder tissues and ten control samples, was downloaded from the Gene Expression Omnibus. The differentially expressed genes (DEGs) in bladder cancer samples compared with the control samples were screened using the Student's t-test method. Functional analysis for the DEGs was analyzed using the Database for Annotation, Visualization, and Integrated Discovery from the Gene Ontology database, followed by the transcription function annotation of DEGs from Tumor-Associated Gene database. Motifs of genes that had transcription functions in promoter region were analyzed using the Seqpos. RESULTS: A total of 1,571 upregulated and 1,507 downregulated DEGs in the bladder cancer samples were screened. ELF3 and MYBL2 involved in cell cycle and DNA replication were tumor suppressors. MEG3, APEX1, and EZH2 were related with the cell epigenetic regulation in bladder cancer. Moreover, HOXB9 and EN1 that have their own motif were the transcription factors. CONCLUSION: Our study has identified several key genes involved in bladder cancer. ELF3 and MYBL2 are tumor suppressers, HOXB9 and EN1 are the main regulators, while MEG3, APEX1, and EZH2 are driving factors for bladder cancer progression.

14.
Pharmacogn Mag ; 10(40): 422-9, 2014 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25422541

RESUMO

BACKGROUND: Fructus Corni (FC), a well-known traditional Chinese medicine (TCM), derived from the dry ripe sarcocarp of Cornus officinalis Sieb. et Zucc (Cornaceae), has been widely prescribed to treat disease in China for centuries. It has attracted increasingly much attention as one of the most popular and valuable herbal medicine in clinic. However, the systematic analysis of the chemical constituents of FC is difficult to determine and remain unclear. MATERIALS AND METHODS: In this work, a rapid, sensitive, and reliable ultra-performance liquid chromatography-electrospray ionization/quadrupole-time-of-flight high-definition mass spectrometry (UPLC-ESI/QTOF/MS) with automated data analysis (MetaboLynx™) in negative ion mode were established to characterize the chemical constituents of FC and simultaneously identify components in blood after oral administration of FC, respectively. The analysis was performed on a Waters UPLC™ HSS T3 (2.1 × 100 mm, 1.8 µm) using gradient elution system. MS/MS fragmentation behaviors were proposed for aiding the structural identification of the components. RESULTS: With optimized conditions, a total of 34 peaks were obtained from FC, 23 of which were tentatively characterized by comparing the retention time and mass spectrometry data and retrieving the reference literatures. Of note, the 25 compounds were identified after oral administration of FC, which might be the potential active components in vivo. CONCLUSION: The present study demonstrates the potential of UPLC-ESI/QTOF/MS approach for the rapid and reliable characterization of the metabolites of natural products.

15.
J Sep Sci ; 37(14): 1742-7, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24777933

RESUMO

The chemical constituents of Simiao Wan (SW), a traditional Chinese medicine preparation, are difficult to determine and remain unclear. To more efficiently detect ions, a multiple data processing approach has been used in the characterization of the compounds. In this study, a rapid and sensitive method based on ultra high performance liquid chromatography with mass spectrometry and the multiple data processing approach was established to characterize the chemical constituents of SW. Ultra high performance liquid chromatography with mass spectrometry coupled with the multiple data processing approach could efficiently remove nonrelated ion signals from accurate mass data. We report the application of the multiple data processing approach for comprehensive detection and rapid identification of chemical constituents of SW. Of note, the total analysis time for separation was less than 20 min without losing any resolution. In the variable, importance in projection plot of orthogonal projection to latent structure-discriminant analysis, a total of 72 ions of interest (37 ions in positive mode, 38 ions in negative mode and three ions in both mode) were extracted or tentatively characterized based on their retention times, exact mass measurement for each molecular ion and subsequent fragment ions. In summary, the methodology proposed in this study could be valuable for the structural characterization and identification of the multiple constituents in the traditional Chinese medicine formula SW.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/análise , Processamento Eletrônico de Dados/métodos , Espectrometria de Massas/métodos
16.
Appl Biochem Biotechnol ; 173(4): 857-69, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24728784

RESUMO

Metabolomics can provide an opportunity to develop the systematic analysis of the metabolites in biological samples and has been increasingly applied to discovering and identifying biomarkers and perturbed pathways. It enables us to better understand the metabolic pathways which can clarify the mechanism of traditional Chinese medicines (TCM). Yinchenhao (YCH, Artemisia annua L), a famous TCM plant, has been used clinically for more than a thousand years to relieve liver diseases in Asia, and its mechanisms are not still completely clear. Here, metabolomic techniques may provide additional insight, and our investigation was designed to assess the effects and possible mechanisms of YCH on α-naphthylisothiocyanate (ANIT)-induced liver injury. Metabolite profiling was performed by ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) combined with pathway analysis and pattern recognition approaches including independent component analysis (ICA) and partial least squares-discriminant analysis (PLS-DA). Biochemistry test was also performed for the liver tissue and plasma samples. The changes in metabolic profiling were restored to their baseline values after YCH treatment according to the ICA score plots. Of note, YCH has a potential pharmacological effect through regulating multiple perturbed pathways to normal state, correlating well to the assessment of biochemistry test. Five different potential biomarkers in the positive mode contributing to the treatment of YCH were discovered. Pathway analysis showed that these metabolites were associated with perturbations in pyrimidine metabolism, primary bile acid biosynthesis, and propanoate metabolism, which may be helpful to further understand the action mechanisms of YCH. It showed that changed biomarkers and pathways may provide evidence to insight into drug action mechanisms and drug discovery.


Assuntos
Artemisia annua/química , Doença Hepática Induzida por Substâncias e Drogas/sangue , Medicamentos de Ervas Chinesas/farmacologia , Isocianatos/toxicidade , Metaboloma/efeitos dos fármacos , Metabolômica/métodos , Naftalenos/toxicidade , Reconhecimento Automatizado de Padrão/métodos , Animais , Biomarcadores/sangue , Medicamentos de Ervas Chinesas/química , Masculino , Medicina Tradicional Chinesa , Ratos , Ratos Wistar
17.
Huan Jing Ke Xue ; 26(3): 118-21, 2005 May.
Artigo em Chinês | MEDLINE | ID: mdl-16124482

RESUMO

The effects of different forms of phosphorus sources on the phosphorus metabolism of Microcystis aeruginosa and attached Pseudomonas sp. were investigated after four substance of different phosphrus forms: NaH2PO4, Natrium-beta-glycerophosphate (NaGly), Ca3(PO4)2 and lecithin were added in the non-phosphorus MA culture. The growth of Microcystis aeruginosa, total phosphorus, alkaline phosphatase activity in water and total phosphorus in Microcystis aeruginosa were measured every day or every two days. Results show that attached Pseudomonas sp. could accelerate the growth of Microcystis aeruginosa, and transform some phosphrus forms which could not be assimilated very easily by Microcystis aeruginosa to some forms as phosphate to utilize for Microcystis aeruginosa. Alkaline phosphatase plays an important role in the utilization of large molecular organic phosphorus by Microcystis aeruginosa and attached Pseudomonas sp.


Assuntos
Fosfatase Alcalina/metabolismo , Microcystis/metabolismo , Fósforo/metabolismo , Pseudomonas/metabolismo , Adesão Celular , Fósforo/farmacologia
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