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1.
J Exp Bot ; 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34487168

RESUMO

Small GTPases play critical roles in the regulation of plant growth and development. However, the mechanism of small GTPases in plant response to virus infection remains largely unknown. Here, a Rho-type GTPase NtRHO1 was identified as one of up-regulated genes after tobacco mosaic virus (TMV) infection. Subcellular localization of NtRHO1 showed that it was localized in the cytoplasm, plasma membrane as well as nucleus. Transient overexpression of NtRHO1 in Nicotiana benthamiana plants accelerated virus reproduction and led to more reactive oxygen species production. By contrast, silencing of NtRHO1 reduced the sensitivity of N. benthamiana plants to TMV-GFP. Further explorations showed that there existed a direct interaction between NtRHO1 and NtWRKY50, a positive regulator of N. benthamiana plants response to virus infection. Yeast one-hybrid and electrophoretic mobility shift assays showed that this regulation was related to NtWRKY50's binding capacity to the WK-box of PR1 promoter, which was weakened by the interaction between NtRHO1 and NtWRKY50. Thus, the role of a novel small GTPase NtRHO1 in the plant-pathogen interaction was explored and its mechanism was proposed.

2.
PLoS One ; 16(8): e0255518, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34339470

RESUMO

China is the key player in the globalization era and is eliminating its intra-national trade barrier. This process will affect interprovincial CO2 flows. This study recalculates interprovincial CO2 flows in China by using the latest MRIO table and applies a gravity model to assess how market segmentation affects interprovincial CO2 flows. Results show that the total volume of interprovincial embodied CO2 flow did not increase excessively from 2007 to 2012, but the pattern of embodied CO2 flow had changed a lot. Market segmentation significantly decreased the interprovincial embodied CO2 flows in China and within its sub-regions. At interregional level, market segmentation's negative effect was significant between Central and Western China. Other variables such as geographical distance showed a significant negative impact on interprovincial embodied CO2 flow in China. On the basis of our results, we raise some relevant policies to deal with the environmental inequality caused by the decrease in market segmentation.

3.
Sensors (Basel) ; 21(16)2021 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-34450917

RESUMO

Monocular depth estimation based on unsupervised learning has attracted great attention due to the rising demand for lightweight monocular vision sensors. Inspired by multi-task learning, semantic information has been used to improve the monocular depth estimation models. However, multi-task learning is still limited by multi-type annotations. As far as we know, there are scarcely any large public datasets that provide all the necessary information. Therefore, we propose a novel network architecture Semantic-Feature-Aided Monocular Depth Estimation Network (SFA-MDEN) to extract multi-resolution depth features and semantic features, which are merged and fed into the decoder, with the goal of predicting depth with the support of semantics. Instead of using loss functions to relate the semantics and depth, the fusion of feature maps for semantics and depth is employed to predict the monocular depth. Therefore, two accessible datasets with similar topics for depth estimation and semantic segmentation can meet the requirements of SFA-MDEN for training sets. We explored the performance of the proposed SFA-MDEN with experiments on different datasets, including KITTI, Make3D, and our own dataset BHDE-v1. The experimental results demonstrate that SFA-MDEN achieves competitive accuracy and generalization capacity compared to state-of-the-art methods.


Assuntos
Semântica
4.
Artigo em Inglês | MEDLINE | ID: mdl-33502155

RESUMO

The pitch-based activated carbon fibers with nickel sulfide nanoparticles (ACF/NiS) were designed by in situ polymerization of ethylene tar with the addition of nickel nitrate followed by melt spinning, stabilization, carbonization, steam activation, and vulcanization processes. The ACF/NiS with hierarchical pore structure and abundant active sites was used as an anode material to improve Coulombic efficiency and increase capacity of potassium-ion batteries (PIBs). The results showed the obtained ACF/NiS with excellent specific surface area of 1552 m2 g-1 and high mesopore volume contribution of 38%, which delivered a high initial Coulombic efficiency of 84.22%, a high capacity of 292.5 mAh g-1, and retained 95.7% capacity retention after 200 cycles at 0.5 A g-1 current density. The NiS provided abundant active sites for the adsorption of potassium-ion, and the rich hierarchical structure shortened the electrolyte penetration path and expanded the storage space of potassium-ion, making it easier to store potassium-ion inside the ACF/NiS anode to obtain a better performance. This work presented one strategy for designing the hierarchical pore structure of pitch-based ACF to boost the capacity storage of PIBs and revealed that ACF-based carbon materials served as potential anodes for high-performance PIBs.

5.
Behav Brain Funct ; 16(1): 10, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33208152

RESUMO

OBJECTIVE: The purpose of this study was to evaluate the effect of astaxanthin (AST) on cognition function, inflammatory response and oxidative stress in vascular dementia (VD) mice. METHOD: VD mice model was established by left unilateral common carotid arteries occlusion (LUCCAO). Following LUCCAO, AST was intragastrically administered for 30 days. Object recognition test and morris water maze test were used to evaluate cognitive function. Hematoxylin and eosin staining was performed to observe the hippocampal neuron structure. Enzyme-linked immunosorbent assay kit and bicinchoninic acid kit were respectively adopted to measure IL-1ß and IL-4 protein expression and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in hippocampus and prefrontal cortex. RESULTS: AST improved the discrimination ability of VD mice. The escape latency and path length of VD mice treated with AST were dramatically reduced. Besides, AST 200 mg/kg enhanced crossing platform time and the number of times crossing the platform quadrant, and alleviated the morphological impairment in VD mice. Moreover, we found that AST inhibited IL-1ß expression and MDA content, whereas promoted IL-4 expression and SOD activity in a dose-dependent manner. CONCLUSION: AST could improve cognitive impairment and hippocampal neurons in VD mice, which may be related to suppression of inflammatory response and oxidative stress.

6.
PLoS One ; 15(8): e0221664, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32857758

RESUMO

With the rapid growth in the Chinese economy in recent decades, household incomes as well as consumption of goods and services have also steadily increased. This has resulted in growing demand for energy consumption across the economy. It has been suggested that consumption upgrades in tandem with substitutions might exert an impact on mitigating this growth. The input-output method was applied in this study to analyze variations in household indirect energy consumption between 1997 and 2012. The impact of consumption substitution on change was also determined using a two-tier structural decomposition analysis, in which the second-tier is a further decomposition based on first-tier results. The results show that the indirect energy use caused by household consumption makes up between 75% and 78% of total household energy demand and that this increased 161.2% over the study period. First-tier decomposition results reveal that this change was mostly caused by household consumption scale and energy intensity effects. Second-tier decomposition results reveal strong evidence for consumption substitution between energy-intensive industries and non-energy-intensive ones and that this can have an impact on reducing household indirect consumption. Household consumption therefore plays a prominent role in total energy consumption. Transforming to non-energy-intensive or services led consumption patterns should therefore be encouraged by the Chinese government in order to achieve conservation goals.


Assuntos
Fontes Geradoras de Energia/história , Fontes Geradoras de Energia/estatística & dados numéricos , China , História do Século XX , História do Século XXI , Humanos , Indústrias , Fenômenos Físicos
7.
J Exp Bot ; 71(18): 5656-5668, 2020 09 19.
Artigo em Inglês | MEDLINE | ID: mdl-32594157

RESUMO

Plant symptoms are derived from specific interactions between virus and host components. However, little is known about viral or host factors that participate in the establishment of systemic necrosis. Here, we showed that helper component proteinase (HCPro), encoded by Chilli veinal mottle virus (ChiVMV), could directly interact with catalase 1 (CAT1) and catalase 3 (CAT3) in the cytoplasm of tobacco (Nicotiana tabacum) plants to facilitate viral infection. In vitro, the activities of CAT1 and CAT3 were inhibited by the interaction between HCPro and CATs. The C-terminus of HCPro was essential for their interaction and was also required for the decrease of enzyme activities. Interestingly, the mRNA and protein level of CATs were up-regulated in tobacco plants in response to ChiVMV infection. Nicotiana tabacum plants with HCPro overexpression or CAT1 knockout were more susceptible to ChiVMV infection, which was similar to the case of H2O2-pre-treated plants, and the overexpression of CAT1 inhibited ChiVMV accumulation. Also, neither CAT1 nor CAT3 could affect the RNA silencing suppression (RSS) activity of HCPro. Our results showed that the interaction between HCPro and CATs promoted the development of plant systemic necrosis, revealing a novel role for HCPro in virus infection and pathogenicity.


Assuntos
Potyvirus , Viroses , Catalase/genética , Peróxido de Hidrogênio , Doenças das Plantas , Tabaco
8.
Biomed Pharmacother ; 115: 108761, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31100542

RESUMO

BACKGROUND: Treatment of psoriasis is always difficult, which requires intensive scientific research. OBJECTIVE: Tripterygium wilfordii Hook F (TwHF) with acitretin(TwHF + acitretin) is normally used in treating psoriasis. This study aimed to investigate the correlation of plasma miR-126 expression with risk and severity of psoriasis, and its predictive value of response to TwHF + acitretin treatment in psoriasis. METHODS: MiRNA-126(MiR-126) expression in plasma was analyzed in psoriasis patients at month 0 (M0), M1, M3 and M6 and in health controls (HCs) at enrollment by qPCR. Psoriasis-affected body surface area (BSA) and Psoriasis Area and Severity Index (PASI) score were used to assess severity and treatment response. RESULTS: Plasma miR-126 levels were decreased in psoriasis patients compared with HCs (P < 0.001), with area under the curve (AUC) of 0.771. MiR-126 expression was negatively correlated with PASI score (P = 0.001), and negatively associated with psoriasis-affected BSA (P = 0.825). At M6, 65.3% and 36.1% patients achieved PASI 50 and 75, respectively. MiR-126 increased at M1, M3 and M6 after TwHF + acitretin treatment when comparing with M0 (all P < 0.001). Meanwhile, miR-126 expression baseline in PASI 50 group declined when comparing with non-PASI 50 group (P < 0.001). Additionally, data revealed that the cause of high miR-126 baseline level was due to unsuccessfully achieving PASI 50 at M6 after TwHF + acitretin treatment (P < 0.001). However, miR-126 baseline expression was not a predictive factor for PASI 75 achievement (P > 0.05). CONCLUSION: Plasma miR-126 expression is negatively correlated with psoriasis risk and severity, and its high baseline level can be used as a biomarker to predict worse clinical response to TwHF + acitretin treatment in psoriasis.


Assuntos
Acitretina/uso terapêutico , Ceratolíticos/uso terapêutico , MicroRNAs/metabolismo , Extratos Vegetais/uso terapêutico , Psoríase/sangue , Psoríase/tratamento farmacológico , Tripterygium/química , Acitretina/administração & dosagem , Adulto , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Ceratolíticos/administração & dosagem , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Extratos Vegetais/administração & dosagem , Extratos Vegetais/química , Psoríase/metabolismo , Fatores de Risco , Resultado do Tratamento
9.
Medicine (Baltimore) ; 97(48): e12961, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30508885

RESUMO

BACKGROUND: Previous studies examining the prognostic value of glucose transporter 1 in breast cancer have yielded inconsistent results. We, therefore, performed a meta-analysis to clarify this issue. METHODS: The research was reported according to the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. Relevant studies were retrieved from PubMed, Web of Science, EMBASE, and Cochrane library. RESULTS: A total of 7 reports with 1861 patients were finally chosen. GLUT1 overexpression was found to be associated with high histological grade (OR = 3.74, 95% CI = 2.45-5.69, P < .001), negative PR status (OR = 0.33, 95% CI = 0.22-0.49, P < .001), and negative estrogen receptor (ER) status (OR = 0.27, 95% CI = 0.17-0.42, P < .001). However, no significant correlation was seen between GLUT1 levels and presence of lymph node metastasis, tumor size or the status of human epidermal growth factor receptor 2 (HER2). Overexpression of GLUT1 also correlated with a poor overall survival (hazard ratio [HR] = 1.65, 95% confidence interval [CI] = 1.17-2.31, P = .004) and disease-free survival (HR = 2.35, 95% CI = 1.4-3.94, P < .001). No evidence of significant publication bias was found. CONCLUSION: This meta-analysis indicates that GLUT1 expression is associated with poor prognostic and a series of clinicopathological features in breast cancer. GLUT1 might be a potential biomarker and therapeutic target in breast cancer.


Assuntos
Neoplasias da Mama/genética , Transportador de Glucose Tipo 1/biossíntese , Biomarcadores Tumorais , Neoplasias da Mama/patologia , Intervalo Livre de Doença , Feminino , Humanos , Metástase Linfática/genética , Metástase Linfática/patologia , Gradação de Tumores , Prognóstico , Receptor ErbB-2/biossíntese , Análise de Sobrevida , Carga Tumoral
10.
ACS Appl Mater Interfaces ; 9(32): 26731-26739, 2017 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-28745482

RESUMO

Photodynamic therapy (PDT) is an auspicious strategy for cancer therapy by yielding reactive oxygen species (ROS) under light irradiation. Here, we have developed near-infrared (NIR) triggered polymer encapsulated upconversion nanoparticles (UCNPs) based on aggregation-induced emission (AIE) characteristics and mitochondria target ability for PDT. The coated AIE polymer as a photosensitizer can be photoactivated by the up-converted energy of UCNPs upon 980 nm laser irradiation, which could generate ROS efficiently in mitochondria and induce cell apoptosis. Moreover, a "sheddable" poly(ethylene glycol) (PEG) layer was easily conjugated at the surface of NPs. The pH-responsive PEG layer shields the surface positive charges and shows stronger protein-resistance ability. In the acidic tumor environment, PEGylated NPs lose the PEG layer and show the mitochondria-targeting ability by responding to tumor acidity. A cytotoxicity study indicated that these NPs have good biocompatibility in the dark but exert severe cytotoxicity to cancer cells, with only 10% cell viability, upon being irradiated with an NIR laser. The AIE nanoparticles are a good candidate for effective mitochondria targeting photosensitizer for PDT.


Assuntos
Nanopartículas , Humanos , Mitocôndrias , Neoplasias , Fotoquimioterapia , Fármacos Fotossensibilizantes
11.
J Mater Chem B ; 5(31): 6277-6281, 2017 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-32264443

RESUMO

Photodynamic therapy (PDT) has received enormous attention due to its high specificity in eliminating the malignant tumor cells without interposing normal cells, as compared with chemotherapy. In this work, we fabricated mitochondria-targeted NPs using a new AIE cross-linked copolymer (PAIE-TPP) with far red/near-infrared (FR/NIR) subcellular bioimaging ability and a high reactive oxygen species (ROS) quantum yield of 77.9%. Such high efficiency ROS generation is desirable to increase the efficiency of PDT. The PAIE-TPP NPs possess great cytocompatibility but exert severe cytotoxicity to cancer cells with only 4% of cell viability under ultralow-power-intensity (10 mW cm-2) light irradiation. The AIE cross-linked copolymer NPs not only emit bright FR/NIR fluorescence for efficient in vitro subcellular-mitochondrial bioimaging but also generate high ROS for PDT.

12.
Huan Jing Ke Xue ; 38(1): 343-351, 2017 Jan 08.
Artigo em Chinês | MEDLINE | ID: mdl-29965065

RESUMO

A pot experiment was conducted to study the effects of four modes of water managements on soil Eh values,bioavailability of soil Cd,migration and accumulation of Cd in rice.These four modes of water managements were moistening throughout the entire period of rice growth (M),moistening before filling stage and flooding after filling stage (M-F),flooding before filling stage and moistening after filling stage (F-M),and flooding throughout the entire period of rice growth (F).The results indicated that the exchangeable contents of Cd increased firstly and then declined with the soil Eh values changing from negative to positive.Compared with M,the other three modes (M-F,F-M,and F) significantly reduced the contents of Cd in all rice tissues,including roots,stems,husks and brown rice.Meanwhile,Cd contents in brown rice due to the treatments of M-F and F were 0.19 mg·kg-1 and 0.10 mg·kg-1,respectively.These Cd contents were lower than the limits of 0.2 mg·kg-1 in national food safety standard (GB 2762-2012).Compared with M,the other three modes significantly decreased Cd accumulation amounts in the aboveground parts of rice and also decreased Cd translocation factors in rice.There were significant positive exponential relations between soil Eh values and Cd accumulation amounts in the aboveground parts of rice,Cd translocation factors in rice,or Cd contents in brown rice.The rice biomass due to M-F treatment reached the maximum among the four modes.It was worthy to be mentioned that although Weiyou 46 was considered a variety rice with high Cd accumulation,Cd content in rice brown of Weiyou 46 could be lower than 0.2 mg·kg-1 in the 5 mg·kg-1 of Cd contaminated soil through proper water managements.In summary,M-F treatment ensured high rice yield with low Cd contents in brown rice and could be recommended as the irrigation mode in rice production.Simultaneously,maintaining soil Eh value between -160--130 mV was also important after the filling stage.


Assuntos
Agricultura/métodos , Cádmio/análise , Oryza/química , Poluentes do Solo/análise , Água , Solo
13.
Environ Sci Pollut Res Int ; 23(20): 20853-20861, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27480164

RESUMO

The objective of the present study was to investigate the effects of rapeseed dregs (RSD, a commonly organic fertilizer in rural China) at application rates of 0, 0.75, 1.5, and 3.0 % on Cd availability in soil and its accumulation in rice plants (Oryza sativa L., Xiangwanxian 12#, and Weiyou 46#) by means of a pot experiment. The results showed that application of RSD resulted in a sharp decrease in the soil TCLP-extractable Cd content. However, the soil TCLP-extractable Cd content in amended soil gradually increased during the rice growing period. Application of RSD significantly increased Cd transport from root to shoot and the amount of Cd accumulated in the aerial part. RSD was an effective organic additive for increasing rice grain yield, but total Cd content in rice grain was also increased. At an application rate of 1.5-3.0 % RSD, the total Cd content in Weiyou 46# brown rice was 0.27-0.31 mg kg-1, which exceeded the standard safe limit (0.2 mg kg-1) and was also higher than that of Xiangwanxian 12# (0.04-0.14 mg kg-1). Therefore, Weiyou 46# had a higher dietary risk than Xiangwanxian 12# with RSD application. We do not recommend planting Weiyou 46# and applying more than 0.75 % RSD in Cd-contaminated paddy fields.


Assuntos
Brassica rapa/química , Cádmio/análise , Fertilizantes/análise , Oryza/crescimento & desenvolvimento , Sementes/química , Poluentes do Solo/análise , Ácidos/análise , Disponibilidade Biológica , Biomassa , Cádmio/metabolismo , China , Oryza/química , Raízes de Plantas/química , Raízes de Plantas/crescimento & desenvolvimento , Brotos de Planta/química , Brotos de Planta/crescimento & desenvolvimento , Solo/química , Poluentes do Solo/metabolismo
14.
Artigo em Inglês | MEDLINE | ID: mdl-26959043

RESUMO

The objectives of the present study were to investigate heavy metal accumulation in 22 vegetable species and to assess the human health risks of vegetable consumption. Six vegetable types were cultivated on farmland contaminated with heavy metals (Pb, Cd, Cu, Zn, and As). The target hazard quotient (THQ) method was used to assess the human health risks posed by heavy metals through vegetable consumption. Clear differences were found in the concentrations of heavy metals in edible parts of the different vegetables. The concentrations of heavy metals decreased in the sequence as leafy vegetables > stalk vegetables/root vegetables/solanaceous vegetables > legume vegetables/melon vegetables. The ability of leafy vegetables to uptake and accumulate heavy metals was the highest, and that of melon vegetables was the lowest. This indicated that the low accumulators (melon vegetables) were suitable for being planted on contaminated soil, while the high accumulators (leafy vegetables) were unsuitable. In Shizhuyuan area, China, the total THQ values of adults and children through consumption of vegetables were 4.12 and 5.41, respectively, suggesting that the residents may be facing health risks due to vegetable consumption, and that children were vulnerable to the adverse effects of heavy metal ingestion.


Assuntos
Poluição Ambiental , Contaminação de Alimentos/análise , Indicadores Básicos de Saúde , Metais Pesados/análise , Poluentes do Solo/efeitos adversos , Poluentes do Solo/análise , Verduras/química , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , China , Humanos , Lactente , Pessoa de Meia-Idade , Medição de Risco , Adulto Jovem
15.
Huan Jing Ke Xue ; 37(10): 4004-4010, 2016 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-29964438

RESUMO

This paper studied the effects of applying two combined amendments LST (limestone+meerschaum+titanium dioxide) and LSF (limestone+meerschaum+ferric sulfate) at different ratios of 0, 1, 2, 4, 8 and 16 g·kg-1 on bioavailability Pb, Cd and As in paddy soil and bioaccumulation in rice plants through a pot experiment planting rice. The results indicated that: 1Compared with the control, applying LST and LSF could both significantly increase soil pH values (P<0.05), and LST increased the soil pH values more. 2Applying combined amendments LST and LSF with the amount of 1-16 g·kg-1 decreased exchangeable contents of soil Pb, Cd and As significantly by 16.8%-88.3%, 22.4%-73.7%, 2.25%-43.8%, and 20.2%-86.9%, 20.7%-51.2%, 18.0%-55.1%, respectively. LST and LSF significantly decreased contents of Pb, Cd and As of rice root, shoot, husk and brown rice. When the applying amount of LST and LSF was 16 g·kg-1, the contents of Pb, Cd and As in brown rice decreased by 50.7%, 64.7%, 34.1%, and 40.7%, 40.7%, 36.2%, respectively. 3The ability of rice organs transferring Pb and As was husk>rice straw>root, and that transferring Cd was husk >root >rice straw. The ability to transfer Pb, Cd and As from rice straw to brown rice was Cd >As >Pb. 4After applying LST and LSF, a significant positive relationship was observed between contents of Pb, Cd and As in brown rice and exchangeable contents of these elements in soil. Between contents of Pb, Cd and As in brown rice and soil pH values, there was a significant negative correlation.


Assuntos
Disponibilidade Biológica , Metais Pesados/análise , Oryza/metabolismo , Poluentes do Solo/análise , Arsênio , Cádmio , Chumbo , Solo
16.
J Anim Sci Biotechnol ; 4(1): 14, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23556436

RESUMO

The study was conducted to compare the effects of XG with AG and BM at different metabolizable energy diets on growth performance, digestive physiology and energy utilization of broilers fed with corn-SBM diet. A 2 × 4 factorial design was used with two basal diets (the positive control group, PC; negative control with ME reduction 100 kcal/kg, NC) and with or without the addition of three exogenous enzymes (0.02% BM; 0.01% AG; 0.05% XG) respectively. 1,200 one-day-old broilers were randomly allocated to 8 treatments with 10 pens of 15 broilers. There was no significant difference on BW, BWG, and FI at 0-21d, 21-42d or 0-42d for diet, enzymes or their interactions, but FI at 22-42d and 0-42d were tend to be decreased with the addition of enzymes. The F/G was significantly improved by the addition of enzymes especially in NC diet. The dietary AME and TME in PC or NC diet were significantly increased by XG or AG in NC diet. The villus length and V/C of ileum were significantly increased by the addition of BM or XG. XG improved the activities of trypsin, chymotrypsin and amylase, BM improved the activity of trypsin at 21d, and AG improved the activity of chymotrypsin at 21d. Comparing to PC diet, the addition of enzymes in PC or NC diet decreased feed cost per kg body weight gain especially in NC diet (except AG in PC diet) with the highest profits for XG in NC diet. In conclusion, supplementation of 0.02% BM or 0.01% AG or 0.05% XG could improve feed conversion of broilers in corn-soybean meal diet by improving energy utilization and digestive physiology, and also supplementation of 0.05% XG had a preferable efficacy in low energy diet.

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