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1.
Int J Syst Evol Microbiol ; 69(6): 1760-1766, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30950783

RESUMO

A motile, Gram-stain-negative, fusiform-shaped bacterium, designated strain T3T, was isolated from rhizosphere soil of Alhagi sparsifolia, collected from Xinjiang, PR China. Strain T3T grew at 15-42 °C, pH 4-9 and 1-6 % (w/v) NaCl concentrations. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain T3T belonged to the genus Pseudomonas and showed highest similarity of 98.6 % to Pseudomonas azotifigens JCM 12708T, followed by Pseudomonas balearica DSM 6083T (97.8 %), Pseudomonas matsuisoli JCM 30078T (97.7 %), Pseudomonas furukawaii KF707T (97.7 %), Pseudomonas tarimensis CCTCC AB 2013065T (97.3 %) and Pseudomonas indica DSM 14015T (97.1 %). Analysis based on concatenated gene sequences of 16S rRNA, rpoB and gyrB further confirmed the phylogenetic assignment of strain T3T. The Genome-to-Genome Distance Calculator results for P. azotifigens JCM12708T and P. balearica DSM 6083T were 28.7±4.4% and 24.1±2.4 %, and the average nucleotide identity scores were 81.3 and 78.1 %. The major polar lipids of strain T3T were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant quinone was Q-9. The major fatty acids comprised summed feature 8 (C18 : 1ω6c/C18 : 1ω7c; 37.7 %), summed feature 3 (C16 : 1ω6c/C16 : 1ω7c; 28.2 %), C16 : 0 (15.6 %), C12 : 0 (7.8 %), C10 : 03-OH (3.0 %) and C12 : 03-OH (2.6 %). The G+C content of the genomic DNA of the type strain was 65.3 mol%. It is obvious from the phylogenetic, phenotypic and chemotaxonomic data that strain T3T represents a novel species of the genus Pseudomonas, for which the name Pseudomonasurumqiensis sp. nov., is proposed. The type strain is T3T (=ACCC 60124T=JCM 32830T).


Assuntos
Fabaceae/microbiologia , Filogenia , Pseudomonas/classificação , Rizosfera , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Genes Bacterianos , Fosfolipídeos/química , Pseudomonas/isolamento & purificação , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
2.
Braz J Med Biol Res ; 52(4): e8217, 2019 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-30970085

RESUMO

The aim of this study was to perform an updated meta-analysis to quantitatively investigate the association between G20210A polymorphism of Prothrombin gene and the risk of retinal vein occlusion (RVO), based on the available publications with inconsistent results. We utilized the Stata software to perform the heterogeneity test, association test, Begg's and Egger's tests, and sensitivity analysis. We searched three on-line databases (PubMed, Embase, and WOS) and obtained a total of 422 articles. Based on our selection criteria, 24 case-control studies were finally enrolled in this overall meta-analysis; a subgroup analysis by the factors ethnicity, control source, and RVO type was done. Through the association test of overall meta-analysis, we did not observe a significant difference between RVO cases and controls under the A vs G (allele) (z=1.49, P=0.137), A vs G (carrier) (z=1.42, P =0.155), GA vs GG (z=1.50, P=0.135), and GA+AA vs GG (z=1.50, P=0.135). Furthermore, we observed similar negative results in the association test of subgroup analysis (all P>0.05). Heterogeneity, Begg's, and Egger's tests excluded the presence of high heterogeneity and publication bias. Statistically stable results were observed in the sensitivity analyses. Based on integrated analysis of the current evidence, Prothrombin gene G20210A polymorphism is likely unrelated to the risk of RVO.


Assuntos
Predisposição Genética para Doença/genética , Polimorfismo Genético/genética , Protrombina/genética , Oclusão da Veia Retiniana/genética , Genótipo , Humanos , Fatores de Risco
3.
Int J Syst Evol Microbiol ; 69(4): 1179-1184, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30775962

RESUMO

A Gram-stain-negative, non-flagellated, rod-shaped bacterium, designated strain T22T, was isolated from rhizosphere soil of Alhagi sparsifolia, collected from Xinjiang, China. Its major fatty acids (>5 %) were iso-C15 : 0, C16 : 1ω5c, iso-C17 : 0-3OH, summed feature 1 (C13 : 0 3-OH/iso-C15 : 1 H) and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). The predominant respiratory quinone was MK-7. The major polar lipids were phosphatidylethanolamine, two aminolipids and four unidentified lipids. The DNA G+C content of the type strain was 53.4 mol%. According to phylogenetic analysis based on 16S rRNA gene sequences, strain T22T was related most closely to Chitinophaga barathri YLT18T (=CCTCC AB 2015054T) with similarity of 97.7 %. However, strain T22T was clearly distinguished from Chitinophaga barathri YLT18T using genome-to-genome distance and average nucleotide identity value calculation, as well as a range of physiological and biochemical characteristics comparisons. It is obvious from the genotypic and phenotypic data that strain T22T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga alhagiae sp. nov., is proposed. The type strain is T22T (=ACCC 60125T=KCTC 62518T).


Assuntos
Bacteroidetes/classificação , Fabaceae/microbiologia , Filogenia , Rizosfera , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Bacteroidetes/isolamento & purificação , Composição de Bases , China , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química
4.
Acta Pharmacol Sin ; 40(8): 1119-1126, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-30760834

RESUMO

Successful prevention and treatment of hypertension depend on the appropriate combination of antihypertensive drug therapy and nondrug lifestyle modification. While most hypertension guidelines recommend moderate- to high-intensity exercise, we decided to explore a mild yet effective type of exercise to add to hypertension management, especially in populations with complications or frailty. After comparing the short-term cardiovascular effects of low-speed walking versus high-speed walking for 3 kilometers (km) (3 km/h versus 6 km/h) in young, healthy volunteers, we delivered low-speed walking (low-intensity walking, 2.5 metabolic equivalents of task, METs) as exercise therapy in 42 prehypertensive and 43 hypertensive subjects. We found that one session of 3 km low-intensity walking exerted a transient pressure-lowering effect as well as a mild negative chronotropic effect on heart rate in both the prehypertensive and hypertensive subjects; these short-term benefits on blood pressure and heart rate were accompanied by a brief increase in urine ß-endorphin output. Then we prescribed regular low-intensity walking with a target exercise dose (exercise volume) of 500-1000 METs·min/week (50-60 min/day and 5-7 times/week) in hypertensive subjects in addition to their daily activities. Regular low-intensity walking also showed mild but significant blood pressure-lowering and heart rate-reducing effects in 7 hypertensive subjects within two months. It is hypothesized that regular low-intensity exercise of the necessary dose could be taken as a pragmatic and supplementary medication for hypertension management.

5.
Braz. j. med. biol. res ; 52(4): e8217, 2019. tab, graf
Artigo em Inglês | LILACS-Express | ID: biblio-1001511

RESUMO

The aim of this study was to perform an updated meta-analysis to quantitatively investigate the association between G20210A polymorphism of Prothrombin gene and the risk of retinal vein occlusion (RVO), based on the available publications with inconsistent results. We utilized the Stata software to perform the heterogeneity test, association test, Begg's and Egger's tests, and sensitivity analysis. We searched three on-line databases (PubMed, Embase, and WOS) and obtained a total of 422 articles. Based on our selection criteria, 24 case-control studies were finally enrolled in this overall meta-analysis; a subgroup analysis by the factors ethnicity, control source, and RVO type was done. Through the association test of overall meta-analysis, we did not observe a significant difference between RVO cases and controls under the A vs G (allele) (z=1.49, P=0.137), A vs G (carrier) (z=1.42, P =0.155), GA vs GG (z=1.50, P=0.135), and GA+AA vs GG (z=1.50, P=0.135). Furthermore, we observed similar negative results in the association test of subgroup analysis (all P>0.05). Heterogeneity, Begg's, and Egger's tests excluded the presence of high heterogeneity and publication bias. Statistically stable results were observed in the sensitivity analyses. Based on integrated analysis of the current evidence, Prothrombin gene G20210A polymorphism is likely unrelated to the risk of RVO.

6.
Oncotarget ; 8(43): 75467-75477, 2017 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-29088882

RESUMO

We performed a meta-analysis to investigate the association between the Factor V G1691A polymorphism and the risk of retinal vein occlusion (RVO). This analysis included 37 studies involving 2,510 cases and 3,466 controls. Factor V G1691A was associated with an increased risk of RVO in the allele, heterozygote, dominant, and carrier models (PA < 0.001, odds ratios >1), but not the homozygote or recessive models (PA > 0.05). Similar results were observed in a meta-analysis of central retinal vein occlusion (CRVO) and when comparing Caucasian subgroups to population-based controls. These data demonstrate that the G/A genotype of Factor V G1691A is associated with an increased risk of RVO/CRVO in a Caucasian population.

7.
J Ophthalmol ; 2017: 7694913, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28386477

RESUMO

Purpose. To describe clinical features and to analyze visual outcome of ocular trauma in Cangzhou in 2012-2015, China. Methods. A retrospective study of ocular trauma cases admitted to Cangzhou Central Hospital from January 2012 till December 2015 was performed. Results. This study included a total of 507 eyes from 478 patients. Four hundred (83.7%) patients were male, with a male-to-female ratio of 5.1 : 1. Mean age was 43.6 ± 18.3 years (5-95 years). The largest age group was 45-59 years old, followed by 30-44 years old, presenting two peaks of the age distribution and accounting for 28.5% and 27.2%, respectively. The most frequent type of injuries was work-related (194, 40.6%) followed by home-related (123, 25.7%). Initial visual acuity (VA) correlated with final VA (Spearman's test, r = 0.703, p = 0.001). The Ocular Trauma Score also correlated with the final VA significantly (Spearman's test, r = 0.802, p = 0.001). Conclusions. Susceptible population of eye injuries were middle- and young-aged working groups, and the proportion of males was higher. The leading two types of ocular trauma were work-related and home-related. Initial VA was a significant predictor of the final VA and the OTS possibly had predictive value in the final VA.

8.
Neurosci Bull ; 33(2): 153-160, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28238115

RESUMO

The aim of this study was to explore the differences between boys and girls in the diagnosis and clinical phenotypes of autism spectrum disorder (ASD) in China's mainland. Children diagnosed with ASD (n = 1064, 228 females) were retrospectively included in the analysis. All children were assessed using the Autism Diagnostic Interview-Revised (ADI-R) and Autism Diagnostic Observation Schedule (ADOS). The results showed that girls scored significantly higher in ADI-R socio-emotional reciprocity than boys, and also scored lower in ADI-R and ADOS restricted and repetitive behaviors (RRBs). Meanwhile, the proportions of girls who satisfied the diagnostic cut-off scores in the ADI-R RRBs domain were lower than in boys (P < 0.05). Our results indicated that girls with ASD show greater socio-emotional reciprocity than boys. Girls also tended to show fewer RRBs than boys, and the type of RRBs in girls differ from those in boys. The ADI-R was found to be less sensitive in girls, particularly for assessment in the RRBs domain.


Assuntos
Transtorno do Espectro Autista , Deficiências do Desenvolvimento/etiologia , Caracteres Sexuais , Transtorno do Espectro Autista/complicações , Transtorno do Espectro Autista/diagnóstico , Transtorno do Espectro Autista/psicologia , Criança , Pré-Escolar , China , Comunicação , Feminino , Humanos , Relações Interpessoais , Masculino , Fenótipo , Escalas de Graduação Psiquiátrica , Estudos Retrospectivos , Estatísticas não Paramétricas , Comportamento Estereotipado/fisiologia
9.
Medicine (Baltimore) ; 94(44): e1641, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26554764

RESUMO

This study was aimed to investigate the fundus changes of patients with pregnancy-induced hypertension syndrome (PIHS) using optical coherence tomography (OCT) technology and contrast sensitivity (CS) tests.Ninety-eight patients with PIHS underwent routine eye examinations including vision correction, fundus examination, OCT, and CS tests. The CS test was performed at low, medium, and high frequency, respectively. Moreover, the difference in CS tests between 2 groups was analyzed by independent-samples T test. The Kruskal-Wallis rank sum test and linear regression model were used to detect the correlation of OCT with CS, respectively. Meanwhile Satterthwaite approximate T test was adopted for pairwise comparisons after nonparametric analysis of variance.The OCT test revealed that 56.76% of the examined eyes showed shallow retinal detachment in the macula lutea and around the optic disk. The differences in CS at each spatial frequency between the case and control group were statistically significant (P < 0.01). Besides, OCT manifestations were associated with CS at each spacial frequency including 1.5, 3, 6, 12, and 18 frequency (P < 0.01). And patients with abnormal manifestations of OCT showed lower CS at each spacial frequency than those without abnormal OCT manifestations. What's more the OCT manifestation 1 showed the greatest impact on CS at each spacial frequency.The results showed that abnormal OCT manifestations were correlated with CS in PIHS. OCT and CS tests might be valuable methods in observing fundus changes for PIHS patients.


Assuntos
Sensibilidades de Contraste , Hipertensão Induzida pela Gravidez/diagnóstico , Retina/patologia , Tomografia de Coerência Óptica/métodos , Adulto , Feminino , Fundo de Olho , Idade Gestacional , Humanos , Hipertensão Induzida pela Gravidez/fisiopatologia , Pessoa de Meia-Idade , Gravidez , Adulto Jovem
10.
Wei Sheng Wu Xue Bao ; 55(1): 33-9, 2015 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-25958680

RESUMO

OBJECTIVE: To find anti-phytopathogen compounds from endophytic fungi associated with the endangered species Heptacodium miconioides. METHODS: Fungi from H. miconioides with antifungal activities were isolated according to the plate growth inhibition method. The fungus with preferable antifungal activities was identified by morphological identification and 5. 8S rRNA sequence analysis. The bioactive metabolites were isolated and purified by chromatographic methods; the structures were determined by spectroscopic analysis. RESULTS: Alternaria solani QZH 10 showed better antifungal activity against Rhizoctorzia solani and Valsa mali with the inhibition rates of 89.1% and 67.9%, respectively. The ethyl acetate crude extract of QZH 10 had strong antifungal activity against Magnaporthe oryzae with the rate of 100. 0% under the concentration of 100 µg/mL. Two antifungal metabolites altersolanol A and 6-O-methylalaternin were isolated and determined from QZH 10. Altersolanol A possessed strong activity against M. oryzae with the inhibition rate of more than 85%, 6-O-methylalaternin had the mightily activity against V. mali with the inhibition rate of 100.0% under the concentration of 100 µg/mL. CONCLUSION: Altersolanol A and 6-O-methylalaternin are potential fungicides originated from microorganisms.


Assuntos
Antifúngicos/metabolismo , Antifúngicos/farmacologia , Caprifoliaceae/microbiologia , Endófitos/isolamento & purificação , Endófitos/metabolismo , Fungos/isolamento & purificação , Doenças das Plantas/microbiologia , Rhizoctonia/efeitos dos fármacos , Antifúngicos/química , Endófitos/classificação , Endófitos/genética , Fungos/classificação , Fungos/genética , Fungos/metabolismo , Dados de Sequência Molecular , Estrutura Molecular , Filogenia , Rhizoctonia/fisiologia , Metabolismo Secundário
11.
Genet Test Mol Biomarkers ; 18(4): 245-52, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24689893

RESUMO

Associations between vascular endothelial growth factor (VEGF) polymorphisms (rs833061, rs1413711, and rs3025039) and risk of age-related macular degeneration (AMD) have been extensively studied, but the currently available results are contentious rather than conclusive. Therefore, we performed the present meta-analysis to further assess the associations. Literature search in PubMed, Embase, and Web of Science databases was conducted until April 2013. The strength of the associations between VEGF polymorphisms and AMD risk was estimated by pooled odds ratios (ORs) and 95% confidence intervals (CIs). Both models of fixed effects and random effects were performed to summarize the pooled ORs. All data were analyzed by Stata software 12.0. The meta-analysis results based on nine case-control studies with 2427 cases and 2037 controls showed that rs833061 had protective effects on AMD risk (TT vs. CT+CC: OR=0.58, 95% CI=0.41-0.81), whereas rs1413711 (TT vs. CT+CC: OR=1.46, 95% CI=1.10-1.93) and rs3025039 (TT vs. CC: OR=1.87, 95% CI=1.15-3.02; TT vs. CT+CC: OR=2.09, 95% CI=1.30-3.37) represented as risk factors for AMD. Subgroup analysis by ethnicity suggested significantly reduced risk in Caucasians (TT vs. CT+CC: OR=0.60, 95% CI=0.36-0.99; T vs. C: OR=0.89, 95% CI=0.78-1.00) and Asians (TT+CT vs. CC: OR=0.57, 95% CI=0.34-0.96; TT vs. CT+CC: OR=0.54, 95% CI=0.33-0.90) for rs833061, yet elevated risk in Caucasians (TT vs. CT+CC: OR=2.05, 95% CI=1.24-3.38) for rs1413711 and in Asians (TT vs. CC: OR=2.06, 95% CI=1.24-3.43; TT vs. CC: OR=2.34, 95% CI=1.42-3.89) for rs3025039. In stratified analysis by type of AMD, rs833061 was observed to decrease wet AMD risk, while rs1413711 and rs3025039 were found to increase the risk of wet AMD. Based on the currently available data, this meta-analysis suggests that the VEGF polymorphisms may be associated with risk of AMD, particularly wet AMD.


Assuntos
Degeneração Macular/genética , Polimorfismo de Nucleotídeo Único , Fator A de Crescimento do Endotélio Vascular/genética , Intervalos de Confiança , Predisposição Genética para Doença , Humanos , Razão de Chances
12.
Mol Vis ; 18: 2096-106, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22876136

RESUMO

PURPOSE: In our previous study, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) played a neuroprotective role in retinal ischemia/reperfusion (I/R) injury in rats. However, the mechanism of NF-κB neuroprotection is still unclear. We hypothesize that p38 mitogen-activated protein kinase (MAPK) is expressed and NF-κB activity induced by p38 MAPK plays a neuroprotective role through antiapoptotic genes (B-cell lymphoma [Bcl]-2 and Bcl-XL) in retinal cells in retinal I/R injury. METHODS: Retinal ischemia was induced by elevating intraocular pressure in rats. After retinal I/R, the p38 MAPK, NF-κB p65, Bcl-2, and Bcl-XL mRNA levels were measured with real-time polymerase chain reaction. NF-κB p65 activity was assessed with NF-κB enzyme-linked immunosorbent assay in retinal I/R injury and after application of the p38 MAPK inhibitor (SB203580). Furthermore, SB203580 and NF-κB p65 short interfering RNA (siRNA) were used in retinal I/R injury to examine the effects on Bcl-2 and Bcl-XL levels and nucleosome release in the retina and cell survival in the ganglion cell layer. RESULTS: The mRNA levels of NF-κB p65 and p38 MAPK reached a peak at 6 h after retinal I/R and then decreased gradually. The mRNA levels of Bcl-2 and Bcl-XL significantly increased at 2, 4, and 6 h, peaked at 8 h, and decreased gradually, but remained at a higher level compared with the normal control, which was accompanied by an increase in NF-κB p65 in nuclear extracts. After application of SB203580, the increase in the NF-κB p65 levels in the nucleus induced with I/R was completely abolished, and the mRNA expression of Bcl-2 and Bcl-XL decreased significantly compared with the I/R controls. In addition, NF-κB p65 siRNA inhibited Bcl-2 and Bcl-XL expression. Inhibition of the p38 MAPK-NF-κB pathway (using SB203580 or NF-κB p65 siRNA) increased retinal nucleosome release and decreased the number of ganglion cells. CONCLUSIONS: These findings provide evidence of crosstalk between p38 MAPK and NF-κB p65 and demonstrate a possible neuroprotective role for the p38 MAPK-NF-κB pathway through Bcl-2 and Bcl-XL in retinal I/R injury in rats.


Assuntos
Traumatismo por Reperfusão/metabolismo , Retina/metabolismo , Células Ganglionares da Retina/metabolismo , Transdução de Sinais/genética , Fator de Transcrição RelA/metabolismo , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo , Animais , Regulação da Expressão Gênica/efeitos dos fármacos , Imidazóis/farmacologia , Masculino , Inibidores de Proteínas Quinases/farmacologia , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Piridinas/farmacologia , RNA Mensageiro/biossíntese , RNA Interferente Pequeno/genética , Ratos , Traumatismo por Reperfusão/genética , Traumatismo por Reperfusão/patologia , Retina/efeitos dos fármacos , Retina/patologia , Células Ganglionares da Retina/efeitos dos fármacos , Células Ganglionares da Retina/patologia , Transdução de Sinais/efeitos dos fármacos , Fator de Transcrição RelA/genética , Proteínas Quinases p38 Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases p38 Ativadas por Mitógeno/genética
13.
Arch Microbiol ; 194(12): 1001-12, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22892578

RESUMO

The seeds of plants are carriers of a variety of beneficial bacteria and pathogens. Using the non-culture methods of building 16S rDNA libraries, we investigated the endophytic bacterial communities of seeds of four hybrid maize offspring and their respective parents. The results of this study show that the hybrid offspring Yuyu 23, Zhengdan958, Jingdan 28 and Jingyu 11 had 3, 33, 38 and 2 OTUs of bacteria, respectively. The parents Ye 478, Chang 7-2, Zheng 58, Jing 24 and Jing 89 had 12, 36, 6, 12 and 2 OTUs, respectively. In the hybrid Yuyu 23, the dominant bacterium Pantoea (73.38 %) was detected in its female parent Ye 478, and the second dominant bacterium of Sphingomonas (26.62 %) was detected in both its female (Ye 478) and male (Chang 7-2) parent. In the hybrid Zhengdan 958, the first dominant bacterium Stenotrophomonas (41.67 %) was detected in both the female (Zheng 58) and male (Chang 7-2) parent. The second dominant bacterium Acinetobacter (9.26 %) was also the second dominant bacterium of its male parent. In the hybrid Jingdan 28, the second dominant bacterium Pseudomonas (12.78 %) was also the second dominant bacterium of its female parent, and its third dominant bacterium Sphingomonas (9.90 %) was the second dominant bacterium of its male parent and detected in its female parent. In the hybrid Jingyu 11, the first dominant bacterium Leclercia (73.85 %) was the third dominant bacterium of its male parent, and the second dominant bacterium Enterobacter (26.15 %) was detected in its male parent. As far as we know, this was the first research reported in China on the diversity of the endophytic bacterial communities of the seeds of various maize hybrids with different genotypes.


Assuntos
Bactérias/classificação , Biodiversidade , Sementes/microbiologia , Zea mays/microbiologia , Bactérias/genética , Quimera/microbiologia , China , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , Zea mays/genética
14.
World J Microbiol Biotechnol ; 28(1): 391-6, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22806816

RESUMO

The spermosphere, an important habitat to the plant micro-ecosystem, has a unique significance to seed microbial ecology, but has been poorly researched. In this study, the mature seeds of reciprocal cross maize (Zea mays L., Nongda108) were collected to investigate the diversity and population succession dynamics of indigenous spermosphere bacteria at 12, 24 and 36 h into seed germination using 16S rDNA library construction. In the spermosphere of Nongda108A (Huang C × 178), the dominant bacteria genera identified were Pseudomonas and Burkholderia. The proportion of Pseudomonas increased from 59.60 to 75.00% then 82.61%; while Burkholderia decreased from 39.39 to 25.00% then 15.22% at 12, 24 and 36 h, respectively. Bacillus, Paenibacillus and Stenotrophomonas were the dominant genera in Nongda108B. The proportion of Paenibacillus after 12, 24 and 36 h into germination decreased from 68.00 to 46.15 to 13.27%, respectively. The proportion of non-Paenibacillus genera increased from 32.00 (Stenotrophomonas) to 53.85 (Bacillus) to 77.55% (Burkholderia) from 12 h to 24 h to 36 h, respectively. Some dominant bacteria genera identified from maize spermosphere have been identified as common PGPR.


Assuntos
Endófitos/isolamento & purificação , Microbiologia de Alimentos , Zea mays/microbiologia , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Sequência de Bases , Biodiversidade , Ecossistema , Endófitos/classificação , Endófitos/genética , Genes Bacterianos , Germinação , Metagenoma , Filogenia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Sementes/microbiologia , Zea mays/crescimento & desenvolvimento
15.
Zhonghua Liu Xing Bing Xue Za Zhi ; 33(4): 386-90, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-22781411

RESUMO

OBJECTIVE: To estimate the prevalence of cataract and its surgical coverage rate together with the burden related to bilateral cataract-blindness, among adults aged 40 or above in Gongshan county of Yunnan province and to evaluate the current cataract status and the efficacy of local cataract prevention program. METHODS: Cluster sampling was used. The protocol consisted of personal interview, pilot study, visual acuity checking, measuring the intraocular pressure; slit lamp microscopy and the fundus of the eye examination etc. Cataract was graded clinically using the Lens Opacity Classification System (LOCS) III. Bilateral cataract-blindness burden, bilateral cataract-blindness burden and cataract surgical coverage rate were calculated respectively, using two different criteria. Odds ratios (OR) were compared among different groups regarding age, gender, education, ethnic group and altitude of living area. RESULTS: Among the 1236 eligible residents, 1116 (90.3%) were enrolled in the present study. The prevalence of cataract was 23.8% among adults aged 40 or order. When the bilateral best refractive vision<3/60 was defined as the blindness criterion, the bilateral cataract-blindness burden showed as 1.3%, and cataract blindness surgical coverage rate was 50.0%. When the bilateral presenting vision<6/60 was defined as the blindness criterion, the bilateral cataract-blindness burden was 25.0%, and cataract blindness surgical coverage rate was 12.9%. The cataract surgical coverage rates were much lower and the bilateral cataract-blindness burden much higher in women, illiterates, living in high altitude areas and those who were aged 70 or above. CONCLUSION: Cataract blindness was a serious public health problem in aged individuals and illiteracy in the residents of the studied areas. Poor prevention programs on cataract called for urgent action to be taken.


Assuntos
Extração de Catarata , Catarata/epidemiologia , Adulto , Idoso , Altitude , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , População Rural
16.
Zhonghua Yan Ke Za Zhi ; 47(9): 791-6, 2011 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-22177123

RESUMO

OBJECTIVE: To estimate the prevalence and causes of visual impairment by a population-based survey conducted in Gongshan County of Yunnan Province. METHODS: Cluster sampling method was used for sample selection. In person interview, pilot study, visual acuity (VA) check, intraocular pressure, slit lamp microscopy and fundus examination were performed. The diagnoses of blindness (VA<0.05) and visual impairment (VA<0.3 to ≥0.05) were based on best-corrected visual acuity in the better eye. The prevalence of visual impairment was calculated as to age, gender, education, ethnic group and altitude of living area. The dominant causes of blindness and visual impairment were then identified. The comparison of prevalence among different group examined by four-fold table Chi-square test, R×2 Chi-square test and trend Chi-square test. RESULTS: Among 3070 eligible residents, 2460 (80.1%) were finally enrolled in the present study. The total prevalence of visual impairment was 6.46%. The bilateral blindness and unilateral blindness was 19 and 46 respectively. The bilateral and unilateral low vision was 49 and 45 respectively. There was no statistical significant difference of prevalence of visual impairment among different ethnic groups (χ2=0.75, P=0.388). There was significantly statistical difference of prevalence of visual impairment among groups who lives in different altitude area (χ2=18.34, P=0.000). High prevalence were also observed in the elder (≥70 years), illiterate and outdoor-workers, which was 2.24%, 4.19%, 5.65% respectively. The leading causes of bilateral blindness was cataract (42.1%, 8/19), corneal opacity (26.3%, 5/19), and retinal abnormality (21.1%, 4/19). The leading cause of bilateral low vision was also cataract (42.9%, 21/49). CONCLUSIONS: Cataract was the dominant cause of visual impairment in Gongshan County of Yunnan Province. The study highlights an urgent need of visual impairment prevention program conducted by local public heath intervention, especially focusing on cataract treatment.


Assuntos
Altitude , Cegueira/epidemiologia , Áreas de Pobreza , Baixa Visão/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , China/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prevalência , População Rural , Adulto Jovem
17.
Zhonghua Er Ke Za Zhi ; 49(8): 607-11, 2011 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-22093425

RESUMO

OBJECTIVE: To explore possible relationship between copy-number variations (CNVs) in 15q11-13, 16p11 and SHANK3 gene by using multiplex ligation-dependent probe amplification (MLPA) and the phenotypes in children with autism and to further explore the clinical application of MLPA to make an etiological diagnosis of Autism. METHODS: The diagnosed of autism was made according to the criteria of the ICD-10 and DSM-IV, with typical cluster of symptoms comprise social disability, communication impairments and repetitious behaviors. MLPA KIT P343-C1 AUTISM-1 was used to detect and describe the incidence of CNVs in these three domains. RESULTS: Among 109 cases collected from 102 autistic pedigrees, 2 individuals had SHANK3 microdeletion, accounting for approximately 2% (2/109) of cases, suggesting the proportion of SHANK3 microdeletion might contribute to typical autism. The phenotypic traits of patients with SHANK3 microdeletions showed homogenicity in severe core symptoms and mental retardation. CONCLUSIONS: SHANK3 microdeletion is an important genetics component for autism, which may explain 2% typical autism cases. SHANK3 microdeletion might explain autistic core symptoms and mental retardation. MLPA is a sensitive and a high throughput technique to detect CNVs in specific DNA segments, which is beneficial for further investigation of etiology of autism.


Assuntos
Transtorno Autístico/genética , Proteínas de Transporte/genética , Variações do Número de Cópias de DNA , Fenótipo , Criança , Pré-Escolar , Feminino , Deleção de Genes , Humanos , Masculino , Proteínas do Tecido Nervoso
18.
Protein J ; 30(1): 66-71, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21221750

RESUMO

We report the characterization of three Superoxide dismutase (sod) genes isolated from a bacterium in the Geobacillus genus. We isolated the bacterium from high-temperature pond mud and used 16S rRNA gene sequence to confirm its identity in the Geobacillus genus. The three genes Mn-sod, Fe/Mn-sod, and Cu/Zn-sod were cloned and analyzed. Their open reading frames are Mn-sod: 615 bp encoding a 204 amino acid protein; Fe/Mn-sod: 1,236 bp encoding a 411 amino acid protein; Cu/Zn-sod: 522 bp encoding a 173 amino acid protein. When these sod genes were expressed in Escherichia coli, only Mn-SOD was able to be purified. The activity of the purified Mn-SOD we got was about 2,730 U/mg. Studies of this Mn-SOD showed that it was thermostable at 60°, had 70% activity at 80° after 2.5 h, and still had 30% activity at 90° after 2.5 h. Mn-SOD activity required the ion Mn²+. Based on gel electrophoresis, we deduced that this Mn-SOD was a homotetramer. No activity was detected after the other two genes (Fe/Mn-sod, Cu/Zn-sod) were expressed in Escherichia coli, but activities were detected when expressed in Pichia pastoris.


Assuntos
Geobacillus/genética , Superóxido Dismutase/química , Superóxido Dismutase/isolamento & purificação , Animais , Escherichia coli/genética , Escherichia coli/metabolismo , Geobacillus/metabolismo , Temperatura Alta , Fases de Leitura Aberta , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo
19.
Int J Syst Evol Microbiol ; 60(Pt 6): 1266-70, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19667385

RESUMO

Four Gram-stain-positive, aerobic or facultatively anaerobic, motile, endospore-forming, rod-shaped bacteria, designated strains FeL05(T), FeL11, Fek19 and Fek21, were isolated from seeds of hybrid rice (Oryza sativa L. Jinyou 611), and their taxonomic positions were determined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four strains were members of the genus Paenibacillus. They showed 95.4 % sequence similarity or less with strains of other Paenibacillus species. The G+C content of strain FeL05(T) was found to be 53.3 mol%. Its predominant respiratory quinone was MK-7. The predominant cellular fatty acids were anteiso-C(15 : 0) (61.7 %), C(16 : 0) (10.9 %), iso-C(16 : 0) (7.0 %), anteiso-C(17 : 0) (6.7 %) and iso-C(15 : 0) (5.2 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain FeL05(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hunanensis sp. nov. is proposed. The type strain is strain FeL05(T) (=ACCC 10718(T) =CGMCC 1.8907(T) =DSM 22170(T)).


Assuntos
Oryza/microbiologia , Paenibacillus/isolamento & purificação , Sementes/microbiologia , Composição de Bases , Benzoquinonas/metabolismo , China , Primers do DNA , Dados de Sequência Molecular , Paenibacillus/classificação , Paenibacillus/genética , Paenibacillus/metabolismo , Peptidoglicano/química , Peptidoglicano/isolamento & purificação , Peptidoglicano/metabolismo , Fenótipo , Filogenia , Reação em Cadeia da Polimerase , RNA Bacteriano/química , RNA Bacteriano/genética , RNA Ribossômico 16S/química , RNA Ribossômico 16S/genética
20.
Zhonghua Yan Ke Za Zhi ; 45(11): 1052-4, 2009 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-20137426

RESUMO

Any disease in visual pathway will affect the visual signal transmission, even more seriously, leading to irreversible blindness. The study of retinal prostheses is that retinal microelectrodes are implanted into different places of retina, and retinal microelectrodes set off electrical signals or deliver neurotransmitters, so as to excite and activate retinal neurons. This review describes the construction, mechanism, way of implantation, influencing factor, advantage and shortcoming, effect of post implantation, biocompatibility of 3 types of retinal prostheses.


Assuntos
Próteses Visuais , Materiais Biocompatíveis , Eletrodos Implantados , Humanos , Microeletrodos , Implantação de Prótese , Retina/cirurgia
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