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1.
Ecohealth ; 13(2): 328-38, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-26813966

RESUMO

This work aimed at evaluating the antifungal susceptibility and production of virulence factors by Candida spp. isolated from sirenians in Brazil. The isolates (n = 105) were recovered from the natural cavities of Amazonian and West Indian manatees and were tested for the susceptibility to amphotericin B, itraconazole, and fluconazole and for the production of phospholipases, proteases, and biofilm. The minimum inhibitory concentrations (MICs) for amphotericin B ranged from 0.03 to 1 µg/mL, and no resistant isolates were detected. Itraconazole and fluconazole MICs ranged from 0.03 to 16 µg/mL and from 0.125 to 64 µg/mL, respectively, and 35.2% (37/105) of the isolates were resistant to at least one of these azole drugs. Concerning the production of virulence factors, phospholipase activity was observed in 67.6% (71/105) of the isolates, while protease activity and biofilm production were detected in 50.5% (53/105) and 32.4% (34/105) of the isolates, respectively. Since the natural cavities of manatees are colonized by resistant and virulent strains of Candida spp., these animals can act as sources of resistance and virulence genes for the environment, conspecifics and other animal species, demonstrating the potential environmental impacts associated with their release back into their natural habitat.


Assuntos
Candida/patogenicidade , Trichechus manatus/parasitologia , Animais , Brasil , Saúde Ambiental , Testes de Sensibilidade Microbiana , Virulência
2.
Can J Microbiol ; 61(10): 763-9, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26308797

RESUMO

The aim of this study was to characterize the yeast microbiota of natural cavities of manatees kept in captivity in Brazil. Sterile swabs from the oral cavity, nostrils, genital opening, and rectum of 50 Trichechus inunguis and 26 Trichechus manatus were collected. The samples were plated on Sabouraud agar with chloramphenicol and incubated at 25 °C for 5 days. The yeasts isolated were phenotypically identified by biochemical and micromorphological tests. Overall, 141 strains were isolated, of which 112 were from T. inunguis (Candida albicans, Candida parapsilosis sensu stricto, Candida orthopsilosis, Candida metapsilosis, Candida guilliermondii, Candida pelliculosa, Candida tropicalis, Candida glabrata, Candida famata, Candida krusei, Candida norvegensis, Candida ciferri, Trichosporon sp., Rhodotorula sp., Cryptococcus laurentii) and 29 were from T. manatus (C. albicans, C. tropicalis, C. famata, C. guilliermondii, C. krusei, Rhodotorula sp., Rhodotorula mucilaginosa, Rhodotorula minuta, Trichosporon sp.). This was the first systematic study to investigate the importance of yeasts as components of the microbiota of sirenians, demonstrating the presence of potentially pathogenic species, which highlights the importance of maintaining adequate artificial conditions for the health of captive manatees.


Assuntos
Microbiota , Trichechus/microbiologia , Animais , Brasil , Candida/isolamento & purificação , Candida/fisiologia , Cryptococcus/isolamento & purificação , Cryptococcus/fisiologia , Feminino , Masculino , Rhodotorula/isolamento & purificação , Rhodotorula/fisiologia , Trichechus inunguis/microbiologia , Trichechus manatus/microbiologia , Trichosporon/isolamento & purificação , Trichosporon/fisiologia
3.
Dis Aquat Organ ; 113(1): 69-73, 2015 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-25667338

RESUMO

Phaeohyphomycoses are emerging and opportunistic diseases caused by dematiaceous fungi that infect many animal species. This paper describes a case of cutaneous phaeohyphomycosis in an Antillean manatee Trichechus manatus manatus caused by Bipolaris hawaiiensis. Blackish skin lesions were observed in an Antillean manatee calf held captive in Brazil. Direct examination of skin scraping from the affected areas revealed the presence of dematious hyphae. Culture of skin fragments led to the isolation and subsequent identification of B. hawaiiensis as the etiologic agent. Treatment with itraconazole for 14 d was effective. Infections by Bipolaris spp. are rare in animals, and this is the first report of B. hawaiiensis in veterinary medicine.


Assuntos
Doenças Transmissíveis Emergentes/microbiologia , Fungos Mitospóricos/isolamento & purificação , Feoifomicose/veterinária , Trichechus manatus/microbiologia , Animais , Antifúngicos/uso terapêutico , Brasil/epidemiologia , Doenças Transmissíveis Emergentes/tratamento farmacológico , Doenças Transmissíveis Emergentes/epidemiologia , Feminino , Itraconazol/uso terapêutico , Fungos Mitospóricos/classificação , Feoifomicose/tratamento farmacológico , Feoifomicose/epidemiologia , Feoifomicose/microbiologia
4.
Anat Rec (Hoboken) ; 297(5): 939-48, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24449600

RESUMO

The morphology and location of lymph nodes from seven species of Odontocetes, of both sexes and different age groups, were described. All animals were derived from stranding events along the North and Northeastern coasts of Brazil. After the identification of lymph nodes in situ, tissue samples were analyzed for light and electron microscopy. Vascular volume density (VVD) and vascular length density (VLD) were evaluated in the mesenteric lymph nodes. Lymph nodes occurred as solitary nodules or in groups, varying in shape and size. In addition to using the nomenclature recommended by Nomina Anatomica Veterinaria, new nomenclatures were suggested based on the lymph nodes topography. Lymph nodes were covered by a highly vascularized and innervated capsule of dense connective tissue, below which muscle fibers were observed, inconsistently, in all studied species. There was no difference in VLD among different age groups. However, VVD was higher in adults. Lymph nodes parenchyma was divided into an outer cortex, containing lymph nodules and germinal centers; a paracortical region, transition zone with dense lymphoid tissue; and an inner medulla, composed of small irregular cords of lymphatic tissue, blood vessels, and diffuse lymphoid tissue. Abundant collagen fibers were observed around arteries and arterioles. Germinal centers were more evident and developed in calves and young animals, being more discrete and sparse in adults. The morphology of lymph nodes in Odontocetes was typical of that observed in other terrestrial mammals. However, new groups of lymph nodes were described for seven species occurring in the Brazilian coast.


Assuntos
Linfonodos/anatomia & histologia , Tecido Linfoide/anatomia & histologia , Animais , Brasil , Cetáceos , Feminino , Masculino
5.
Vet Parasitol ; 173(1-2): 116-22, 2010 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-20638793

RESUMO

This study represents the first survey of the parasitic fauna of cetaceans off the northeastern coast of Brazil. Parasites were collected from 82 animals rescued from the states of Ceará to Bahia, including the archipelago of Fernando de Noronha. A total of 14 species of cetaceans were evaluated: Sotalia guianensis, Stenella sp., Stenella clymene, Stenella longirostris, Stenella coeruleoalba, Stenella frontalis, Megaptera novaeangliae, Peponocephala electra, Steno bredanensis, Kogia breviceps, Kogia sima, Globicephala macrorhynchus, Tursiops truncatus, Physeter macrocephalus and Lagenodelphis hosei. The parasites were fixed and preserved in 70% ethanol or alcohol-formalin-acetic acid solution (AFA), clarified in phenol and mounted on slides for morphological identification. In total, 11 species and 8 genera of endo- and ectoparasites were identified: Halocercus brasiliensis, Halocercus kleinenbergi, Stenurus globicephalae, Halocercus sp., Anisakis sp., Crassicauda sp. (Nematoda), Phyllobothrium delphini, Monorygma grimaldii, Scolex pleuronectis, Strobicephalus triangularis, Tetrabothrius forsteri, Tetrabothrius sp., Trigonocotyle sp., Diphyllobothrium sp. (Cestoda), Campula sp. (Trematoda), Bolbosoma sp. (Acanthocephala), Cyamus boopis, Syncyamus pseudorcae and Xenobalanus globicipitis (Crustacea). The identification of some species represented novel records for the country and increased the occurrence of some parasites to new hosts. The use of standardized methodologies for collecting and evaluating a larger number of animals is essential for a better understanding of host-parasite relationships in cetaceans and their use as biological indicators in the region.


Assuntos
Cetáceos , Ectoparasitoses/veterinária , Helmintíase Animal/epidemiologia , Animais , Oceano Atlântico/epidemiologia , Brasil/epidemiologia , Cestoides , Crustáceos , Ectoparasitoses/epidemiologia , Ectoparasitoses/parasitologia , Feminino , Helmintíase Animal/parasitologia , Masculino , Nematoides , Trematódeos
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