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1.
Parasitol Int ; 106: 103035, 2025 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-39875001

RESUMO

Blastocystis is a protist that infects both human and animal hosts worldwide. This study aimed to investigate the presence of Blastocystis in humans and domestic animals living in a periurban (PZ) and rural zone (RZ) in Northeastern Argentina and to assess their relation to socio-environmental conditions and hygiene practices as risk factors for human infection. In addition, we identified Blastocystis subtypes to evaluate the risk of zoonotic transmission. A total of 563 fecal specimens were collected from 289 humans, principally children, and 274 animals. Samples were examined by coprological examination and further analysis by real-time PCR and sequencing were performed. A semi-structured questionnaire was applied to obtain socio-environmental and hygiene practices data. The results showed an overall prevalence of 41.6 % in children and 10.2 % in animals. Non-schooled children (OR = 0.54) and children from urban area (OR = 0.55) showed a lower risk of infection. Molecular analyses revealed five subtypes (ST1, ST2, ST3, ST5, and ST7) present in humans and four subtypes (ST1, ST5, ST7 and ST10) in animals. The overlap of STs between humans and domestic animals in the same household (STs 1, 5 and 7) suggests potential zoonotic transmission, underscoring the role of infected animals as a potential risk factor for human infections. Our results can inform local health authorities to promote policies aimed at reducing transmission, emphasizing the need for direct molecular-level assessments of other common environmental sources, such as water and soil, highlighting the importance of adopting a One Health approach to better understand Blastocystis circulation.

2.
Acta Trop ; 261: 107500, 2024 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-39708899

RESUMO

Hantavirus Pulmonary Syndrome (HPS) is a Pan-American emerging infectious disease with a high mortality rate caused by rodent-borne viruses of the genus Orthohantavirus. In Argentina, almost half of the HPS infections occur in the northwestern endemic region. In this study, we evaluated rodent composition, abundance, and antibody prevalence in wild rodents in three subtropical sites: primary forest, secondary forest, and crop fields. From February 2022 to June 2023, we settled a total of 150 traps for three nights every two months reaching a capture-effort of 5,400 trap-nights. We took blood samples from all captured rodents, registered morphological measures, then marked and released. We captured 103 rodents of 5 species (Oligoryzomys chacoensis, Calomys fecundus, Calomys musculinus, Akodon simulator, and Euryoryzomys legatus). The overall antibody prevalence was 10.18 %; with C. fecundus and A. simulator being the species most frequently found to present antibodies, and they were also the dominant species in the captured rodent community. The secondary forest was, by far, the most productive site with 83.5 % of all captures, followed by crop fields (10.68 %) and primary forest (5.82 %). We found that antibody prevalence for C. fecundus was positively correlated to body mass (weight) and size (total length). Another important result of our work is that we report presence of hantavirus antibodies in E. legatus, which represents the first record for this species. These results highlight the importance of continuous monitoring of rodents populations and provide a framework for the planning and implementation of public health prevention campaigns of field workers.

3.
One Health Outlook ; 6(1): 27, 2024 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-39616377

RESUMO

BACKGROUND: Rodents play essential ecological roles but are also significant reservoirs of zoonotic pathogens, posing risks to humans. Individuals with frequent occupational contact with rodents face an elevated risk of exposure to rodent-borne diseases. This study examines the knowledge, risk perceptions, and practices of Chilean environmental consultants (ECs) concerning rodents and rodent-borne diseases. METHODS: A 32-item questionnaire, which focused primarily on biologists, veterinarians, and environmental engineers, was administered. The questionnaire included closed, open-ended, and semi-open-ended questions. Data analyses, performed using R software, involved calculating frequencies and proportions for questions related to knowledge of rodents and rodent-borne diseases, rodent management and handling, biosafety procedures, and training. Additionally, generalized linear models (GLMs) were used to assess knowledge of rodent hosts and diseases, whereas correspondence analysis was used to examine associations between EC characteristics (undergraduate fields of study and years of experience) and responses regarding risk perception and the importance of zoonotic disease training. RESULTS: Completed questionnaires were received from 206 ECs. Although the ECs demonstrated a strong awareness of hantavirus cardiopulmonary syndrome, identifying it as a significant rodent-borne threat, knowledge gaps were evident regarding the specific role of certain rodent reservoir species and other rodent-borne diseases in Chile. For example, leptospirosis was well recognized, particularly among veterinarians, whereas emerging zoonoses such as scrub typhus was rarely mentioned. The study also revealed the frequent use of live trapping and direct handling of rodents, resulting in injuries to 32% of the respondents. Despite the widespread use of personal protective equipment, key elements such as respiratory protection and disposable suits were underutilized, and inadequate cleaning practices for traps were reported. Nevertheless, ECs expressed a willingness to participate in zoonotic disease training, underscoring the need for further education. CONCLUSIONS: This study highlights the critical need for ongoing education for ECs on rodent reservoirs, diseases, and biosafety measures to enhance their safety and reduce the risk of zoonotic infections. The findings offer valuable insights for developing strategies aimed at improving awareness and strengthening biosafety practices within this occupational group.

4.
Artigo em Inglês | MEDLINE | ID: mdl-39545595

RESUMO

INTRODUCTION: In Brazil, the emergence of feline sporotrichosis, a neglected subcutaneous mycosis primarily transmitted by cats and caused by the fungus Sporothrix brasiliensis, has been monitored via molecular tools. This approach underscores the geographic expansion of this disease and highlights the critical role of molecular surveillance in understanding its epidemiology and guiding public health interventions. METHODS: We investigated three domestic cats (Felis catus) with multiple skin lesions in Picos, Piauí, Brazil. The cats were examined, and samples were collected for laboratory diagnosis, including cytological evaluation, fungal culture, and molecular characterisation. The molecular analysis involved a one-tube multiplex probe-based qPCR assay for the diagnosis of Sporothrix species, mating-type determination, and amplified fragment length polymorphism (AFLP, EcoRI-GA/MseI-AG) to assess genetic similarity and potential origin. RESULTS: All three cats were diagnosed with sporotrichosis caused by the hypervirulent S. brasiliensis. A probable case of zoonotic transmission has been reported in a 12-year-old girl who developed Parinaud oculoglandular syndrome after contact with one of the cats with sporotrichosis. The molecular analysis revealed that the cat isolates were MAT 1-2 and formed a single cluster according to the AFLP analysis, suggesting direct transmission (cat-cat) and a potential founder effect. The isolates were also closely related to strains from Pernambuco and Southeast Brazil, indicating a possible introduction from these regions. CONCLUSIONS: Identifying S. brasiliensis in Piauí emphasises the need for increased awareness and control measures to prevent further spread. The predominance of the MAT1-2 idiomorphs and the genetic similarity among outbreak isolates suggest clonal expansion, which could have significant implications for public health and veterinary practices. Considering its zoonotic potential and environmental adaptability, a One Health approach is crucial for managing and controlling the spread of cat-transmitted sporotrichosis.

5.
Front Public Health ; 12: 1466981, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-39606082

RESUMO

Introduction: Although occupational exposure to Coxiella burnetii has been studied previously, the zoonotic risk in wildlife environments remains unclear and has yet to be fully established. Methods: Accordingly, the present study aimed to serologically assess professionals with daily contact with free-living and captive wildlife in Paraná State, Brazil, along with the potential associated risk factors for C. burnetii exposure. Results: Overall, 25 out of 309 (8.1%) wildlife professionals were seropositive, including 6/54 (11.1%) national and 7/125 (5.6%) state park employees, 6/92 (6.5%) zookeepers, and 6/38 (15.8%) animal service workers, with titers ranging from 32 to 128. No statistical association was found between seropositivity and associated risk factors, including the working location. Discussion: Our results differ from those of previous studies in Brazil, which found 8/893 (0.9%) indigenous, 1/18 (5.5%) police officers, and 44/200 (22.0%) former Black slaves to be seropositive. This study is the first serological investigation of C. burnetii among park rangers, zookeepers, and animal service workers in Brazil, showing no statistically significant risk factors for seropositivity. As the seroprevalence in this study was higher than that in previous surveys of healthy (asymptomatic) human populations, C. burnetii exposure may also be an occupational risk for wildlife professionals owing to their contact with the natural environment in Brazil.


Assuntos
Animais Selvagens , Coxiella burnetii , Exposição Ocupacional , Febre Q , Febre Q/epidemiologia , Humanos , Brasil/epidemiologia , Coxiella burnetii/isolamento & purificação , Animais , Exposição Ocupacional/estatística & dados numéricos , Adulto , Feminino , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Animais Selvagens/microbiologia , Estudos Soroepidemiológicos , Zoonoses , Anticorpos Antibacterianos/sangue
6.
Emerg Infect Dis ; 30(12): 2532-2543, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39592268

RESUMO

To explore hantavirus infection patterns in Latin America, we conducted molecular and serologic hantavirus investigations among 3,400 febrile patients from Peru during 2020-2021. Reverse transcription PCR indicated that a patient from Loreto, in the Peruvian Amazon, was positive for Rio Mamore hantavirus (serum, 3.8 × 103 copies/mL). High genomic sequence identity of 87.0%-94.8% and phylogenetic common ancestry with a rodent-associated Rio Mamore hantavirus from Loreto in 1996 indicated endemicity. In 832 samples from Loreto, hantavirus incidence based on IgM ELISA of pooled Sin Nombre (SNV) and Andes virus (ANDV) nucleoproteins and immunofluorescence assay-based end-point titration using SNV/ANDV/Hantaan/Puumala/Saarema/Dobrava/Seoul hantaviruses was 0.5%. Across 3 ecologically distinct departments in Peru, SNV/ANDV IgG ELISA/IFA-based reactivity was 1.7%, suggesting circulation of antigenically distinct New World hantaviruses. Testing for arboviruses, nonendemic pathogens, and antigen-free ELISA corroborated nonspecific reactivity in 2 IgG and several IgM ELISA-positive serum samples. Hantavirus diagnostics and surveillance should be strengthened in Peru ad across Latin America.


Assuntos
Anticorpos Antivirais , Doenças Endêmicas , Infecções por Hantavirus , Orthohantavírus , Filogenia , Humanos , Peru/epidemiologia , Infecções por Hantavirus/epidemiologia , Infecções por Hantavirus/virologia , Orthohantavírus/genética , Orthohantavírus/classificação , Orthohantavírus/imunologia , Orthohantavírus/isolamento & purificação , Anticorpos Antivirais/sangue , Masculino , Adulto , Feminino , Adolescente , Criança , Pessoa de Meia-Idade , Adulto Jovem , Pré-Escolar , Idoso , Lactente , Animais , Imunoglobulina M/sangue
7.
Emerg Infect Dis ; 30(12): 2559-2566, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39592388

RESUMO

The epidemiologic effects of Zika virus (ZIKV) sexual transmission in virus-endemic countries remain unclear. We conducted a 2-level, linear mixed-effects logistic regression analysis by using a recently acquired population-based ZIKV and chikungunya virus (CHIKV) serologic dataset obtained from persons residing in Northeast Brazil (n = 2,070 participants). We adjusted mathematical models for housing type and age of participants; the models indicated a significantly higher likelihood of ZIKV seropositivity among persons engaged in a sexual relationship within the same household (odds ratio 1.25 [95% CI 1.00-1.55]; p = 0.047), regardless of their partner's ZIKV serostatus, and among participants with a ZIKV-seropositive sex partner within the same household (odds ratio 1.54 [95% CI 1.18-2.01]; p = 0.002). CHIKV was also modeled as a control; no sex-associated effects were observed for CHIKV serology. Inclusion of ZIKV sexual transmission in prevention and control strategies is urgently needed, particularly in ZIKV-endemic regions.


Assuntos
Parceiros Sexuais , Infecção por Zika virus , Zika virus , Humanos , Infecção por Zika virus/epidemiologia , Infecção por Zika virus/transmissão , Brasil/epidemiologia , Masculino , Feminino , Zika virus/imunologia , Adulto , Adulto Jovem , Adolescente , Pessoa de Meia-Idade , Comportamento Sexual , Doenças Endêmicas , Doenças Virais Sexualmente Transmissíveis/epidemiologia , Doenças Virais Sexualmente Transmissíveis/transmissão , Estudos Soroepidemiológicos , Anticorpos Antivirais/sangue , Febre de Chikungunya/epidemiologia , Febre de Chikungunya/transmissão
8.
Emerg Infect Dis ; 30(12): 2629-2632, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39592404

RESUMO

We found Mycobacterium leprae, the most common etiologic agent of Hansen disease or leprosy, in tissues from 9 (18.75%) of 48 nine-banded armadillos (Dasypus novemcinctus) collected across continental Ecuador. Finding evidence of a wildlife reservoir is the first step to recognizing leprosy zoonotic transmission pathway in Ecuador or elsewhere.


Assuntos
Tatus , Hanseníase , Mycobacterium leprae , Mycobacterium leprae/isolamento & purificação , Mycobacterium leprae/genética , Animais , Tatus/microbiologia , Equador/epidemiologia , Hanseníase/microbiologia , Hanseníase/veterinária , Hanseníase/transmissão , Hanseníase/epidemiologia , Reservatórios de Doenças/microbiologia , Animais Selvagens/microbiologia
9.
Emerg Infect Dis ; 30(11): 2375-2380, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39356574

RESUMO

In early 2024, explosive outbreaks of Oropouche virus (OROV) linked to a novel lineage were documented in the Amazon Region of Brazil. We report the introduction of this lineage into Colombia and its co-circulation with another OROV lineage. Continued surveillance is needed to prevent further spread of OROV in the Americas.


Assuntos
Infecções por Bunyaviridae , Orthobunyavirus , Filogenia , Colômbia/epidemiologia , Humanos , Orthobunyavirus/genética , Orthobunyavirus/classificação , Infecções por Bunyaviridae/epidemiologia , Infecções por Bunyaviridae/virologia , Surtos de Doenças , Brasil/epidemiologia
10.
Viruses ; 16(10)2024 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-39459932

RESUMO

Studies have demonstrated the susceptibility of companion animals to natural infection with SARS-CoV-2. Using quantitative reverse transcription polymerase chain reaction and sequencing analyses, this study investigated SARS-CoV-2 RNA excretion in pets in households with infected owners. Oropharyngeal and rectal swabs were collected from dogs and cats in Parana, Southern Brazil, between October 2020 and April 2021. Viral RNA was detected in 25% of cats and 0.98% of dog oropharyngeal swabs; however, systemic, respiratory, and gastrointestinal signs were absent. Complete viral genomes belonged to the Gamma lineage. Phylogenetic analyses indicated that pet samples were probably derived from human-positive cases in Parana. Viral excretion in the oropharynx was more frequent in cats than in dogs. Mutations in the S protein characteristic of Gamma strains were present in all sequenced SARS-CoV-2 strains. The receptor-binding domain of these Brazilian strains did not show any additional mutations not reported in the Gamma strains. Mutations in NSP6, NSP12, and N proteins previously mapped to strains that infect deer or minks were detected. This study highlights the importance of actively monitoring the SARS-CoV-2 strains that infect pets with continued viral exposure. Monitoring genetic changes is crucial because new variants adapted to animals may pose human health risks.


Assuntos
COVID-19 , Doenças do Gato , Doenças do Cão , Filogenia , RNA Viral , SARS-CoV-2 , Eliminação de Partículas Virais , Gatos , Animais , Cães , COVID-19/virologia , COVID-19/veterinária , COVID-19/epidemiologia , SARS-CoV-2/genética , RNA Viral/genética , Humanos , Brasil/epidemiologia , Doenças do Cão/virologia , Doenças do Cão/epidemiologia , Doenças do Gato/virologia , Doenças do Gato/epidemiologia , Animais de Estimação/virologia , Mutação , Genoma Viral , Glicoproteína da Espícula de Coronavírus/genética , Orofaringe/virologia , Características da Família
11.
Emerg Infect Dis ; 30(11): 2400-2403, 2024 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-39447175

RESUMO

We report a case of Burkholderia mallei causing glanders in a 73-year-old patient from the Northeast Region of Brazil. The patient was hospitalized with severe pneumonia. PCR and genomic sequencing confirmed B. mallei in pleural drainage. Genotyping revealed a novel genotype, emphasizing the need for genetic surveillance in zoonotic infections.


Assuntos
Burkholderia mallei , Mormo , Idoso , Humanos , Brasil , Burkholderia mallei/genética , Burkholderia mallei/isolamento & purificação , Genótipo , Mormo/microbiologia , Mormo/diagnóstico , Filogenia
12.
P R Health Sci J ; 43(3): 111-118, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39269761

RESUMO

OBJECTIVE: Monkeypox is a viral zoonotic disease endemic to West and Central Africa; it has been reported in more countries during the last decade than in the previous 40 years. In 2022 a multinational outbreak occurred. This change in the epidemiology of the virus may represent an evolutionary adaptation. The purpose of this study is to analyze the molecular aspects of Monkeypox virus (MPXV) disease that may explain the latter's change in epidemiology during the 2022 outbreak. METHODS: From July 2022 through December 2022, the period of the outbreak, a narrative review was conducted on the available literature, with a total of 271 articles published in the MEDLINE/PubMed and LILACS databases being examined. The chosen articles were organized using the search and reference manager Mendeley Desktop 1.19.4. Duplicates and articles that did not meet the study's objective were eliminated, resulting in the selection of 49 articles for the present review. DISCUSSION: MPXV resurgence poses challenges due to waning immunity and changing epidemiological patterns. Recent outbreaks show different transmission routes, affecting new demographics. Genomic evolution, vaccination history, and potential new animal reservoirs complicate containment efforts. Continued surveillance and vaccination are crucial for control. CONCLUSIONS: It seems possible that MPXV has (re-)emerged to occupy the ecological niche left by the smallpox virus. Mutations of the apolipoprotein B mRNA editing enzyme, catalytic subunit 3G motif, in MPXV clade IIb since 2017 may explain the epidemiological change that has occurred in recent years. This pattern could be due to sustained transmission in a new host or a new route of infection.


Assuntos
Surtos de Doenças , Monkeypox virus , /epidemiologia , Humanos , Monkeypox virus/genética , Animais , Zoonoses/epidemiologia , Zoonoses/virologia
13.
medRxiv ; 2024 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-39228722

RESUMO

Background: Cystic echinococcosis (CE) is a widespread neglected zoonotic disease caused by Echinococcus granulosus sensu lato (EG) with a global burden of control in the billions of dollars. E. granulosus' life cycle involves definitive, intermediate, and humans as dead-end hosts. Echinococcosis control programs use strategies that focus on any of these hosts. We aimed to provide a comprehensive and up-to-date overview of the EG control interventions worldwide. Methods: We conducted a scoping review by mapping all studies on interventions for EG control following the Arksey and O'Malley Framework. We screened identified articles, and charted and coded selected papers. We classified the data based on target host, type of study, and control mechanism. We described the efficacy or safety outcomes, and the associated barriers/facilitators for the intervention. Critical appraisal was conducted. Results: From 7,853 screened studies, we analyzed 45: seven centered on human interventions, 21 on animals, and 17 on both. Studies on humans focused on educational strategies and human CE monitoring. The studies on animals were field trials and most were based on Praziquantel (PZQ) for dogs. Studies focused on both animals and humans had, in general, more participants, lasted longer, and covered larger geographical areas. Overall, the quality of studies was moderate to low. Conclusions: Available evidence suggests that long-term interventions aimed at both animals and humans can achieve significant reduction in EG transmission, particularly when PZQ treatment for dogs is included. Higher quality evidence, standardization of methodologies, and better reporting on post-intervention outcomes are necessary for drawing stronger conclusions. Further evidence is needed to assess the sustainability and scalability of control measures. Nonetheless, an integrative One Health approach is essential for overcoming the multiple challenges associated with sustaining long-term control efforts for Echinococcosis.

14.
Emerg Infect Dis ; 30(10): 2211-2214, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39320235

RESUMO

Fort Sherman virus (FSV) was isolated in Panama in 1985 from a US soldier. We report a case of human FSV infection in a febrile patient from northern coastal Peru in 2020. FSV infections spanning ≈35 years and a distance of 2,000 km warrant diagnostics, genomic surveillance, and investigation of transmission cycles.


Assuntos
Filogenia , Humanos , Peru/epidemiologia , Masculino , Adulto , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/diagnóstico , Infecções por Picornaviridae/virologia , História do Século XXI
15.
Acta Parasitol ; 69(4): 1961-1966, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-39278997

RESUMO

PURPOSE: This study aimed to investigate the occurrence of leishmaniasis in domestic rabbits (Oryctolagus cuniculus) in the state of Sergipe, Brazil, and to evaluate the associated clinical signs. METHODS: A total of 31 rabbits from urban and rural areas were clinically examined using cytological, immunological, and serological tests. Blood and cytological samples were collected and analysed for the presence of Leishmania parasites and antibodies. Immunochromatographic tests were used to screen for anti-Leishmania antibodies, and cytological analysis of skin lesions was performed to detect the presence of Leishmania amastigotes. RESULTS: Of the rabbits tested, 19.35% were reactive in the anti-Leishmania antibody screening, and 3.33% tested positive for Leishmania amastigotes in skin lesion cytology. Clinical signs included cachexia, lymphadenomegaly, dehydration, apathy, dermatitis, ophthalmopathy, and alopecia. Cytological analysis revealed pyogranulomatous inflammation with Leishmania amastigotes present. The findings suggest that leishmaniasis is present among domestic rabbits in this region CONCLUSION: This study demonstrates the presence of leishmaniasis in domestic rabbits in the Northeast of Brazil. The findings underline the importance of early diagnosis and intervention in preventing the spread of the disease, and highlight the need for further research into the role of rabbits as potential reservoirs of Leishmania.


Assuntos
Leishmaniose , Animais , Coelhos/parasitologia , Brasil/epidemiologia , Leishmaniose/veterinária , Leishmaniose/epidemiologia , Leishmaniose/parasitologia , Anticorpos Antiprotozoários/sangue , Leishmania/isolamento & purificação , Leishmania/imunologia , Masculino , Feminino , Pele/parasitologia , Pele/patologia
16.
Comp Immunol Microbiol Infect Dis ; 113: 102230, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39236397

RESUMO

The CMNR group comprises bacteria of the genera Corynebacterium, Mycobacterium, Nocardia, and Rhodococcus and share cell wall and DNA content characteristics. Many pathogenic CMNR bacteria cause diseases such as mastitis, lymphadenitis, and pneumonia in farmed animals, which cause economic losses for breeders and represent a threat to public health. Traditional diagnosis in CMNR involves isolating target bacteria on general or selective media and conducting metabolic analyses with the assistance of laboratory biochemical identification systems. Advanced mass spectrometry may also support diagnosing these bacteria in the clinic's daily routine despite some challenges, such as the need for isolated bacteria. In difficult identification among some CMNR members, molecular methods using polymerase chain reaction (PCR) emerge as reliable options for correct specification that is sometimes achieved directly from clinical samples such as tracheobronchial aspirates and feces. On the other hand, immunological diagnostics such as the skin test or Enzyme-Linked Immunosorbent Assay (ELISA) for Mycobacterium tuberculosis yield promising results in subclinical infections with no bacterial growth involved. In this review, we present the methods most commonly used to diagnose pathogenic CMNR bacteria and discuss their advantages and limitations, as well as challenges and perspectives on adopting new technologies in diagnostics.


Assuntos
Animais Domésticos , Mycobacterium , Animais , Animais Domésticos/microbiologia , Mycobacterium/isolamento & purificação , Mycobacterium/genética , Mycobacterium/patogenicidade , Corynebacterium/isolamento & purificação , Corynebacterium/genética , Corynebacterium/patogenicidade , Reação em Cadeia da Polimerase , Rhodococcus/isolamento & purificação , Rhodococcus/genética , Nocardia/isolamento & purificação , Nocardia/genética , Ensaio de Imunoadsorção Enzimática
17.
Biomedica ; 44(3): 416-424, 2024 08 29.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-39241245

RESUMO

INTRODUCTION: Abdominal and neural angiostrongyliasis caused by Angiostrongylus costaricensis and A. cantonensis, respectively, are zoonotic diseases involving snails as intermediate hosts. Colombia has already reported human cases, and the increasing distribution of Lissachatina fulica and Cornu aspersum raises public health concerns due to the potential risk of disease transmission in areas where parasites and hosts coexist. OBJECTIVE: To identify the presence of Angiostrongylus spp. in snail species L. fulica and C. aspersum in Antioquia, Colombia. MATERIALS AND METHODS: This prospective cross-sectional study had a population of 5,855 L. fulica and C. aspersum snails captured in the ten towns of the Valle de Aburrá (Antioquia, Colombia), 169 samples were collected in 28 sampling points. Lung tissues of the collected snails were dissected and analyzed to detect Angiostrongylus spp. through molecular techniques. RESULTS: Angiostrongylus spp. were identified in both L. fulica and C. aspersum. Angiostrongylus costaricensis was detected in 18 pooled prevalence of 30% (95% CI = 19.2-43.3), and Medellín was the municipality with the highest number of positive samples (33.3%). Seventy-two-point-two percent of the positive places reported the presence of rodents. None of the tests were positive for A. cantonensis. CONCLUSION: Our findings provide important insights into the epidemiology and distribution of Angiostrongylus spp. in Antioquia, Colombia. The identification of these parasitic nematodes in L. fulica and C. aspersum highlights the potential role of these snails as intermediate hosts in the transmission of Angiostrongylus spp. infections in the Valle de Aburrá, with implications for human and veterinary health.


Introducción: La angiostrongiliasis abdominal y neura ­causadas por Angiostrongylus costaricensis y A. cantonensis, respectivamente­ son zoonosis que involucran caracoles como huéspedes intermediarios. Colombia ya ha reportado casos en humanos y la ampliación de la distribución de Lissachatina fulica y Cornu aspersum aumenta la preocupación en salud pública debido al riesgo potencial de transmisión en áreas donde los parásitos y sus huéspedes coexisten. Objetivo: Identificar la presencia de Angiostrongylus spp. en caracoles de las especies L. fulica y C. aspersum en Antioquia (Colombia). Materiales y métodos: Se llevó a cabo un estudio transversal prospectivo con una población de 5.855 caracoles de L. fulica o C. aspersum, capturados en diez ciudades del valle de Aburrá; 169 muestras fueron recolectadas en 28 puntos de muestreo. Se disecaron los tejidos pulmonares de los caracoles y se emplearon técnicas moleculares para detectar la presencia de Angiostrongylus spp. Resultados: Angiostrongylus costaricensis fue detectado en 18 muestras agrupadas (30 %; IC95%: 19,2-43,3), tanto en L. fulica como en C. aspersum. Medellín fue el municipio con el mayor número de muestras positivas (33,3 %). El 72,2 % de los lugares positivos reportaron la presencia de roedores. Ninguna de las pruebas fue positiva para A. cantonensis. Conclusión: Estos hallazgos brindan información importante sobre la distribución de Angiostrongylus spp. en Antioquia (Colombia). La identificación de estos nemátodos en L. fulica y C. aspersum resalta el papel potencial de estos caracoles como huéspedes intermediarios en la transmisión de infecciones por Angiostrongylus en el valle de Aburrá, con implicaciones para la salud humana y veterinaria.


Assuntos
Angiostrongylus , Caramujos , Infecções por Strongylida , Animais , Colômbia/epidemiologia , Angiostrongylus/isolamento & purificação , Estudos Transversais , Caramujos/parasitologia , Estudos Prospectivos , Infecções por Strongylida/epidemiologia , Infecções por Strongylida/parasitologia , Infecções por Strongylida/veterinária , Humanos
18.
Biomédica (Bogotá) ; Biomédica (Bogotá);44(3): 416-424, jul.-set. 2024. tab, graf
Artigo em Inglês | LILACS, COLNAL | ID: biblio-1574107

RESUMO

Introduction. Abdominal and neural angiostrongyliasis caused by Angiostrongylus costaricensis and A. cantonensis, respectively, are zoonotic diseases involving snails as intermediate hosts. Colombia has already reported human cases, and the increasing distribution of Lissachatina fulica and Cornu aspersum raises public health concerns due to the potential risk of disease transmission in areas where parasites and hosts coexist. Objective. To identify the presence of Angiostrongylus spp. in snail species L. fulica and C. aspersum in Antioquia, Colombia. Materials and methods. This prospective cross-sectional study had a population of 5,855 L. fulica and C. aspersum snails captured in the ten towns of the Valle de Aburrá (Antioquia, Colombia), 169 samples were collected in 28 sampling points. Lung tissues of the collected snails were dissected and analyzed to detect Angiostrongylus spp. through molecular techniques. Results. Angiostrongylus spp. were identified in both L. fulica and C. aspersum. Angiostrongylus costaricensis was detected in 18 pooled prevalence of 30% (95% CI = 19.2-43.3), and Medellín was the municipality with the highest number of positive samples (33.3%). Seventy-two-point-two percent of the positive places reported the presence of rodents. None of the tests were positive for A. cantonensis. Conclusion. Our findings provide important insights into the epidemiology and distribution of Angiostrongylus spp. in Antioquia, Colombia. The identification of these parasitic nematodes in L. fulica and C. aspersum highlights the potential role of these snails as intermediate hosts in the transmission of Angiostrongylus spp. infections in the Valle de Aburrá, with implications for human and veterinary health.


Introducción. La angiostrongiliasis abdominal y neura -causadas por Angiostrongylus costaricensis y A. cantonensis, respectivamente- son zoonosis que involucran caracoles como huéspedes intermediarios. Colombia ya ha reportado casos en humanos y la ampliación de la distribución de Lissachatina fulica y Cornu aspersum aumenta la preocupación en salud pública debido al riesgo potencial de transmisión en áreas donde los parásitos y sus huéspedes coexisten. Objetivo. Identificar la presencia de Angiostrongylus spp. en caracoles de las especies L. fulica y C. aspersum en Antioquia (Colombia). Materiales y métodos. Se llevó a cabo un estudio transversal prospectivo con una población de 5.855 caracoles de L. fulica o C. aspersum, capturados en diez ciudades del valle de Aburrá; 169 muestras fueron recolectadas en 28 puntos de muestreo. Se disecaron los tejidos pulmonares de los caracoles y se emplearon técnicas moleculares para detectar la presencia de Angiostrongylus spp. Resultados. Angiostrongylus costaricensis fue detectado en 18 muestras agrupadas (30 %; IC95%: 19,2-43,3), tanto en L. fulica como en C. aspersum. Medellín fue el municipio con el mayor número de muestras positivas (33,3 %). El 72,2 % de los lugares positivos reportaron la presencia de roedores. Ninguna de las pruebas fue positiva para A. cantonensis. Conclusión. Estos hallazgos brindan información importante sobre la distribución de Angiostrongylus spp. en Antioquia (Colombia). La identificación de estos nemátodos en L. fulica y C. aspersum resalta el papel potencial de estos caracoles como huéspedes intermediarios en la transmisión de infecciones por Angiostrongylus en el valle de Aburrá, con implicaciones para la salud humana y veterinaria.


Assuntos
Humanos , Zoonoses , Infecções por Strongylida , Angiostrongylus , Caramujos , Colômbia , Moluscos
19.
Emerg Infect Dis ; 30(10): 2149-2154, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-39190550

RESUMO

We identified 3 clades of dengue virus serotype 3 belonging to genotype III isolated during 2019-2020 in Jamaica by using whole-genome sequencing and phylogenomic and phylogeographic analyses. The viruses likely originated from Asia in 2014. Newly expanded molecular surveillance efforts in Jamaica will guide appropriate public health responses.


Assuntos
Vírus da Dengue , Dengue , Filogenia , Sorogrupo , Vírus da Dengue/genética , Vírus da Dengue/classificação , Jamaica/epidemiologia , Humanos , Dengue/virologia , Dengue/epidemiologia , Genoma Viral , Genótipo , Filogeografia , Sequenciamento Completo do Genoma
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