RESUMO
The human inner ear has an intricate spiral shape often compared to shells of mollusks, particularly to the nautilus shell. It has inspired many functional hearing theories. The reasons for this complex geometry remain unresolved. We digitized 138 human cochleae at microscopic resolution and observed an astonishing interindividual variability in the shape. A 3D analytical cochlear model was developed that fits the analyzed data with high precision. The cochlear geometry neither matched a proposed function, namely sound focusing similar to a whispering gallery, nor did it have the form of a nautilus. Instead, the innate cochlear blueprint and its actual ontogenetic variants were determined by spatial constraints and resulted from an efficient packing of the cochlear duct within the petrous bone. The analytical model predicts well the individual 3D cochlear geometry from few clinical measures and represents a clinical tool for an individualized approach to neurosensory restoration with cochlear implants.
Assuntos
Ducto Coclear/anatomia & histologia , Modelos Estatísticos , Osso Petroso/anatomia & histologia , Gânglio Espiral da Cóclea/anatomia & histologia , Lâmina Espiral/anatomia & histologia , Ligamento Espiral da Cóclea/anatomia & histologia , Exoesqueleto/anatomia & histologia , Exoesqueleto/ultraestrutura , Animais , Autopsia , Variação Biológica Individual , Ducto Coclear/fisiologia , Ducto Coclear/ultraestrutura , Audição/fisiologia , Humanos , Imageamento Tridimensional/instrumentação , Imageamento Tridimensional/métodos , Modelos Anatômicos , Nautilus/anatomia & histologia , Nautilus/ultraestrutura , Osso Petroso/fisiologia , Gânglio Espiral da Cóclea/fisiologia , Gânglio Espiral da Cóclea/ultraestrutura , Lâmina Espiral/fisiologia , Lâmina Espiral/ultraestrutura , Ligamento Espiral da Cóclea/fisiologia , Ligamento Espiral da Cóclea/ultraestruturaRESUMO
CONCLUSIONS: Homeostasis of endolymph volume is a complex mechanism, in which the endolymphatic sac (ES) may play an important role. OBJECTIVES: To elucidate the effect of acute endolymphatic hydrops (EH) on the ES and to gain further information about the volume and pressure regulative function of the ES. MATERIALS AND METHODS: Distilled water was injected into the middle ear cavity of adult CBA/J mice. The ESs were studied morphologically by light and transmission electron microscopy. RESULTS: Mild EH was found, particularly in the upper turn of the cochlea. Acute EH led to an increase in the size of the ES lumen, accompanied by collapse of the lateral intercellular spaces and dense perisaccular tissue, changes which had reversed 2 h after the injection.
Assuntos
Hidropisia Endolinfática/patologia , Saco Endolinfático/patologia , Doença Aguda , Animais , Cóclea/patologia , Ducto Coclear/ultraestrutura , Espaço Extracelular/fisiologia , Homeostase/fisiologia , Camundongos , Camundongos Endogâmicos CBA , Microscopia Eletrônica de TransmissãoRESUMO
OBJECTIVES: To study the distribution of polylactic/glycolic acid-encapsulated iron oxide nanoparticles (PLGA-NPs) in chinchilla cochleae after application on the round window membrane (RWM). STUDY DESIGN AND SETTING: Six chinchillas (12 ears) were equally divided into controls (no treatments) and experimentals (PLGA-NP with or without magnetic exposure). After 40 minutes of PLGA-NP placement on the RWM, perilymph was withdrawn from the scala tympani. The RWM and cochleae were fixed with 2.5% glutaraldehyde and processed for transmission electron microscopy. RESULTS: Nanoparticles were found in cochleae with or without exposure to magnet forces appearing in the RWM, perilymph, endolymph, and multiple locations in the organ of Corti. Electron energy loss spectroscopy confirmed iron elements in nanoparticles. CONCLUSION: The nanoparticles were distributed throughout the inner ear after application on the chinchilla RWM, with and without magnetic forces. SIGNIFICANCE: PLGA-NP applied to the RWM may have potential for sustained therapy to the inner ear.
Assuntos
Materiais Biocompatíveis/farmacocinética , Cóclea/metabolismo , Ácido Láctico/farmacocinética , Nanopartículas , Ácido Poliglicólico/farmacocinética , Polímeros/farmacocinética , Animais , Membrana Basilar/metabolismo , Membrana Basilar/ultraestrutura , Chinchila , Cóclea/ultraestrutura , Ducto Coclear/metabolismo , Ducto Coclear/ultraestrutura , Dextranos , Endolinfa/metabolismo , Compostos Férricos/farmacocinética , Óxido Ferroso-Férrico/farmacocinética , Indicadores e Reagentes/farmacocinética , Ferro/farmacocinética , Magnetismo , Nanopartículas de Magnetita , Microscopia Eletrônica de Transmissão , Órgão Espiral/metabolismo , Órgão Espiral/ultraestrutura , Óxidos/farmacocinética , Perilinfa/metabolismo , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Janela da Cóclea/metabolismo , Janela da Cóclea/ultraestruturaRESUMO
Dominant white spotting W/W(v) and W(v)/W(v) mice are well-known mutants that lack strial intermediate cells in their cochlea and manifest hereditary sensorineural hearing loss. We recently reported marked thickening of and IgG deposition on the basement membrane of strial capillaries in W/W(v) mutant mice, similar to observations made in aged animals and in animals with autoimmune sensorineural hearing loss. The present study aimed to clarify the age-dependent changes in these pathological findings of strial capillaries in the W/W(v) mice. Male WBB6F1 +/+ and dominant white spotting W/W(v) mutant mice were sacrificed by transcardiac perfusion with paraformaldehyde solution. The cochlear ducts were isolated and subjected to light- and electron-microscopy, immunohistochemistry, immunoelectron microscopy. Alternatively, lanthanum chloride tracer examination in the isolated cochlear ducts was performed in order to compare the permeability of the strial capillaries between +/+ and W/W(v) mice. In the W/W(v) mice, thickening of and IgG deposition on the basement membrane of strial capillaries were observed as early as 1 week after birth and became more noticeable with age. Deposited IgG was preferentially localized to the thickened basement membrane and was also observed in partially the intercellular space between adjacent of endothelial cells. In addition, pinocytotic vesicles both in the apical and basal lesions of such cells also showed IgG deposition. Lanthanum chloride was retained along apical plasma membrane of the endothelial cells in the +/+ mice but penetrated through the endothelial layer in the W/W(v) mice. These results indicate that active transport via pinocytotic vesicles as well as increased permeability of strial capillaries in the W/W(v) mice occur in the early stage after birth, resulting in the morphological alterations in the strial capillaries of these mice.
Assuntos
Capilares/patologia , Ducto Coclear/metabolismo , Genes Dominantes , Estria Vascular/patologia , Envelhecimento/metabolismo , Envelhecimento/patologia , Animais , Membrana Basal/metabolismo , Capilares/metabolismo , Capilares/ultraestrutura , Membrana Celular/metabolismo , Ducto Coclear/citologia , Ducto Coclear/ultraestrutura , Células Endoteliais/ultraestrutura , Imunoglobulina G/metabolismo , Imuno-Histoquímica , Lantânio , Masculino , Camundongos , Camundongos Mutantes , Microscopia Eletrônica , Microscopia Imunoeletrônica , Permeabilidade , Estria Vascular/metabolismo , Estria Vascular/ultraestruturaRESUMO
OBJECTIVE: In order to providing information for related research, the ultrastructure of microvessel in chick cochlea was investigated. METHOD: Transmission electronic microscope and scanning electronic microscope were used to observe the ultrastructure of microvessel in cochleas of 10 chicks. RESULT: None typical blood capillary with thin--wall was found in chick cochleas, none microvessel was found within basilar membrane, the wall of microvessel in chick cochleas was thick, many lays of fibrils and pericytes and its processes were lined alternately from the endothelial cells to the peripheric basement membrane. Every fold of tegmentem vasculosum contained a microvessel. The ultrastructure of microvessel in spiral ganglion was similar to that of tegmentem vasculosum. The processes of pericyte were netted each other on the surface of microvessel. CONCLUSION: The ultrastructure and the distribution of microvessels in chick cochlea are differented significantly to that of cochlea in mammal. The significance should be investigated further.
Assuntos
Ducto Coclear/irrigação sanguínea , Microcirculação/ultraestrutura , Anatomia Comparada , Animais , Embrião de Galinha , Ducto Coclear/ultraestrutura , Endotélio Vascular/ultraestrutura , Feminino , Masculino , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Membrana Tectorial/irrigação sanguínea , Membrana Tectorial/ultraestruturaRESUMO
The tegmentum vasculosum of the avian cochlear duct mimics the stria vascularis of the mammalian cochlear duct in both location and structure, and previous studies indicate that it may be its functional counterpart with regard to endolymph synthesis. In the present study, we report on the enzymatic activity and ultrastructural localization of the Na+,K+-ATPase in the tegmentum vasculosum of the duckling. Na+,K+-ATPase activity was determined by measuring K+-dependent, ouabain-sensitive p-nitrophenyl phosphatase (p-NPPase) activity in homogenates of dissected regions of the cochlear duct. The ultrastructural localization of the Na+,K+-ATPase was identified using K+-dependent, ouabain-sensitive, p-NPPase cytochemistry. Specific enzyme activity was localized primarily in homogenates of the tegmentum vasculosum (2.27 micromol p-nitrophenyl phosphate/mg protein/min) when compared to homogenates of the entire cochlear duct (0.69 micromol p-nitrophenyl phosphate/mg protein/min). Reaction product for p-NPPase was localized primarily along the basolateral plasma membrane folds of the dark cells. The cytochemical deposits appeared to be located exclusively on the cytoplasmic side of the plasma membrane. The light cells were devoid of reaction product. Biochemical and cytochemical localization of p-NPPase activity on the basolateral plasma membrane folds of the dark cells of the tegmentum vasculosum in conjunction with the ultrastructural morphology of these cells is compatible with a Na+,K+-ATPase-dependent ion transport function related to endolymph synthesis.
Assuntos
Ducto Coclear/enzimologia , Ducto Coclear/ultraestrutura , Patos/anatomia & histologia , Patos/metabolismo , ATPase Trocadora de Sódio-Potássio/metabolismo , 4-Nitrofenilfosfatase/metabolismo , Animais , Endolinfa/metabolismo , Histocitoquímica , Mamíferos , Microscopia Eletrônica , Especificidade da EspécieRESUMO
The tegmentum vasculosum of the duckling consists of a highly folded epithelium which extends over the dorsal and lateral walls of the cochlear duct, separating the scala media from the scala vestibuli. This epithelium consists of two distinct cell types, dark cells and light cells, and is well vascularized. The surface of the epithelium is formed by a mosaic of alternating dark and light cells. The goblet-shaped dark cells have an electron-dense, organelle-rich cytoplasm, and are expanded basally by extensive basolateral plasma membrane infoldings, within which are numerous mitochondria. Dark cells are isolated from each other and from the capillaries within the epithelium by intervening light cells. In contrast, columnar light cells exhibit an electron-lucent, organelle-poor cytoplasm and may extend from the underlying capillaries to the endolymphatic surface. Light cells contain abundant, coated endocytic vesicles on their apical surfaces and are bound, apically, to other light cells or to dark cells by tight junctions and desmosomes. Laterally, light cells are linked to each other either by complex, fluid-filled membrane interdigitations or by extensive gap junctions. Plasma membrane interdigitations and obvious, fluid-filled intercellular spaces characterize the lateral borders between light and dark cells. Vascular corrosion casting reveals the three-dimensional anatomy of the cochlear vasculature. A continuous arteriolar loop fed by anterior and posterior cochlear arterioles encircles the cochlear duct. The rich capillary beds of the tegmentum vasculosum are supplied by arching arterioles arising from this loop. These capillaries are the continuous type and are situated primarily within the core of the epithelium or along its border with the scala vestibuli. The structure and blood supply of the tegmentum vasculosum are characteristic of an epithelium involved in active transport.
Assuntos
Ducto Coclear/irrigação sanguínea , Ducto Coclear/ultraestrutura , Patos/anatomia & histologia , Animais , Ducto Coclear/metabolismo , Molde por Corrosão , Patos/metabolismo , Endolinfa/metabolismo , Mamíferos , Microscopia Eletrônica , Especificidade da EspécieRESUMO
We produced drill-induced damage of the auditory ossicles of guinea pigs to study changes over time in the permeability of the blood vessels of the stria vascularis to horseradish peroxidase (HRP). In group A, the stimulus was applied for 10 seconds after intravenous injection of HRP. In group B, it was applied for 30 seconds, and in group C, for 60 seconds. The cochlea was fixed with 2% glutaraldehyde perfused through the round window, and the guinea pigs were then decapitated. The stria vascularis of the basal and third turns was examined. The leakage of HRP from the blood vessels of the stria vascularis significantly increased in relation to the duration of the stimulus in both the basal and third turns. The damage to intermediate cells also tended to be in relation to the duration of the stimulus. Extravascular permeation of HRP took place through the tubules in the endothelial cytoplasm. The vibratory stimulation presumably opened channels that are not normally open.
Assuntos
Permeabilidade Capilar/fisiologia , Ducto Coclear/irrigação sanguínea , Ducto Coclear/ultraestrutura , Modelos Animais de Doenças , Bigorna/lesões , Estria Vascular/ultraestrutura , Vibração/efeitos adversos , Animais , Orelha Média/cirurgia , Endotélio Vascular/ultraestrutura , Cobaias , Perda Auditiva Neurossensorial/etiologia , Peroxidase do Rábano Silvestre/administração & dosagem , Peroxidase do Rábano Silvestre/farmacocinética , Injeções Intravenosas , Complicações Pós-Operatórias/etiologia , Fatores de TempoAssuntos
Ducto Coclear/metabolismo , Proteínas de Ligação a DNA , Surdez/metabolismo , Proteínas de Membrana Transportadoras , Potássio/metabolismo , Fatores de Transcrição/metabolismo , Animais , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Cóclea/metabolismo , Ducto Coclear/ultraestrutura , Surdez/genética , Surdez/patologia , Endolinfa/metabolismo , Potenciais Evocados Auditivos do Tronco Encefálico , Proteínas da Matriz Extracelular , Proteínas do Olho/genética , Proteínas do Olho/metabolismo , Ligação Genética , Células Ciliadas Auditivas/citologia , Células Ciliadas Auditivas/fisiologia , Humanos , Transporte de Íons , Camundongos , Mutação , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Fatores do Domínio POU , Canais de Potássio/genética , Canais de Potássio/metabolismo , Proteínas/genética , Proteínas/metabolismo , Transportadores de Sulfato , Fatores de Transcrição/genética , Cromossomo XRESUMO
Megalin is an endocytic receptor predominantly expressed in the kidney proximal tubule cells. In the present study, localization of megalin was examined using a post-embedding immunogold method in the rat cochlear duct. Marginal cells of the stria vascularis were labeled on the apical surface, but not on the basolateral surface. This localization pattern resembles kidney proximal tubule cells. Immunoreactivity was also detected on various other cells, including epithelial cells of the spiral prominence and epithelial cells of Reissner's membrane. In contrast, virtually no gold particles were seen on intermediate cells and basal cells of the stria vascularis, mesothelial cells of Reissner's membrane or fibrocytes in the lateral wall. Also unlabeled were cells in the tympanic wall of the cochlear duct, including sensory cells and supporting cells of the organ of Corti. The present findings show the involvement of megalin in endocytosis of marginal cells and are suggestive of different uptake mechanisms for aminoglycosides in the kidney proximal tubule cells and in the cochlear sensory cells.
Assuntos
Ducto Coclear/metabolismo , Ducto Coclear/ultraestrutura , Glicoproteínas de Membrana/metabolismo , Animais , Orelha Média/metabolismo , Orelha Média/ultraestrutura , Complexo Antigênico da Nefrite de Heymann , Túbulos Renais Proximais/metabolismo , Microscopia Imunoeletrônica , Ratos , Ratos Wistar , Distribuição TecidualRESUMO
OBJECTIVES: To inquire into whether the different inner ear tissue antigens could cause different kind of hearing loss, and to find out the disorder positions of auditory system. METHODS: The basilar membrane (BM), spiral ligament (SL), and spiral ganglion (SG) of guinea pigs were removed for making antigens, respectively. Then, we used these antigens to immune guinea pigs. The special humour and cellular immune reaction, hearing function, and inner ear histopathological changes were observed. RESULTS: In BM-antigen and SL-antigen immune group, the various degrees of cochlear microphonic potential disturbance, recruitment, and immune pathological inflammation in cochlear duct and stria vascularis were found. In SG-antigen immune group, auditory nerve compound action potential changes were prominent, and the inner ear pathological damage mainly existed in cochlear axis vessels or surround areas, and SG. CONCLUSIONS: It was confirmed that the inner ear antigens come from different part of auditory system, which could cause cochlear or retrocochlear autoimmune disease.
Assuntos
Doenças Autoimunes , Perda Auditiva Neurossensorial , Perda Auditiva Neurossensorial/etiologia , Animais , Autoanticorpos , Doenças Autoimunes/etiologia , Doenças Autoimunes/patologia , Doenças Autoimunes/fisiopatologia , Membrana Basilar/imunologia , Ducto Coclear/ultraestrutura , Potenciais Microfônicos da Cóclea , Potenciais Evocados Auditivos , Cobaias , Perda Auditiva Neurossensorial/patologia , Perda Auditiva Neurossensorial/fisiopatologia , Gânglio Espiral da Cóclea/imunologia , Estria Vascular/ultraestruturaRESUMO
Ion channels on the apical membrane of epithelial cells (the surface facing the endolymph) of acutely isolated Reissner's membrane from guinea-pig cochlea were investigated by using patch-clamp technique in cell-attached and inside-out configurations. Three types of ion channel were identified: namely, a stretch-activated nonselective cation, a chloride and a potassium channel. When the pipette was filled with high-K+ endolymph-like solution, the most significant channel activity was nonselective cation channels (85/110, 77% patches). The current versus voltage relationship was linear with a unitary conductance of 22.1 +/- 0.4 pS and reversal potential (Vr) of 2.3 +/- 0.8 mV (n = 18). The channel exhibited a lower conductance (14.0 +/- 0.6 pS, n = 8) to Ca2+. The open probability was low (NPo approximately 0.1) in cell-attached configuration under +60 mV pipette potential and increased when the membrane was stretched with negative pressure. The channel was blocked by 10 microM extracellular Gd3+. The two other types of channels were a small voltage-sensitive Cl- channel (6.0 +/- 0.3 pS; 91/99, 92% patches) and a K+ channel (approximately 30 pS; 29/191, 15% patches). These channels might play roles in the regulation of cell volume, in balancing the hydrostatic pressure across Reissner's membrane and in maintaining the electrochemical composition of endolymph.
Assuntos
Canais de Cloreto/metabolismo , Ducto Coclear/metabolismo , Canais Iônicos/metabolismo , Canais de Potássio/metabolismo , Animais , Ducto Coclear/ultraestrutura , Endolinfa/metabolismo , Endolinfa/fisiologia , Células Epiteliais/metabolismo , Células Epiteliais/ultraestrutura , Cobaias , Microscopia Eletrônica , Técnicas de Patch-Clamp , Pressão , SoftwareRESUMO
Localization of the immunoreactivity in the lateral wall of the rabbit cochlear duct was examined using a post-embedding immunogold method with a polyclonal antiserum raised against the rabbit parotid Na-K-Cl cotransporter. In the stria vascularis, the labeling was significant on the basolateral membrane infolding of marginal cells, whereas no labeling was seen on the luminal membrane of these cells. Immunoreactivity was also detected on the cell membranes of various other cells. These include fibrocytes of the spiral ligament and the spiral prominence, and vascular endothelial cells in the stria vascularis and the spiral ligament. In contrast, virtually no gold particles were seen on the membrane of intermediate cells, basal cells of the stria vascularis, the epithelial cells of the spiral prominence, or Reissner's membrane. Our result on the localization of the Na-K-Cl cotransporter in marginal cells is consistent with electrophysiological studies (Wangemann et al. (1995) Hear. Res. 84, 19-29). Our result on fibrocytes is discussed in relation to K+ circulation into endolymph from perilymph (Schulte and Steel (1994) Hear. Res. 78, 65-76).
Assuntos
Proteínas de Transporte/análise , Cloretos/metabolismo , Ducto Coclear/metabolismo , Potássio/metabolismo , Sódio/metabolismo , Animais , Especificidade de Anticorpos , Membrana Basilar/metabolismo , Proteínas de Transporte/metabolismo , Membrana Celular/metabolismo , Ducto Coclear/ultraestrutura , Endolinfa/metabolismo , Endotélio Vascular/citologia , Endotélio Vascular/metabolismo , Feminino , Imuno-Histoquímica , Transporte de Íons/fisiologia , Masculino , Perilinfa/metabolismo , Coelhos , Simportadores de Cloreto de Sódio-Potássio , Gânglio Espiral da Cóclea/citologia , Gânglio Espiral da Cóclea/metabolismo , Estria Vascular/citologia , Estria Vascular/metabolismo , Estria Vascular/ultraestruturaRESUMO
The ultrastructural morphology of the interface region between the stria vascularis (SV) and spiral ligament (SL) was examined in the neonatal rat cochlea via transmission electron microscopy. At postnatal day (PND) 3, morphology of both basal cells and fibrocytes was simple and immature. Only a small number of fibrocytes was observed in the SL. Intercellular junctions between basal cells and fibrocytes, and between adjacent fibrocytes, were few. At PND 7, the number of fibrocytes increased, and more organelles appeared within their cytoplasm. From PND 11 to 14, nuclei of the basal cells appeared to be more spindle-shaped and contained more heterochromatin. The cytoplasm of the fibrocytes was pale, and a greater number of cytoplasmic vesicles and mitochondria emerged. More intercellular junctions were observed between basal cells and fibrocytes at the interface region and between fibrocytes in the SL. By PND 21, the morphology of basal cells and fibrocytes and their intercellular junctions appeared to be adult-like. These morphological observations correlate with previous reports on the functional maturation of the developing rat cochlea.
Assuntos
Cóclea/ultraestrutura , Ducto Coclear/ultraestrutura , Estria Vascular/ultraestrutura , Animais , Animais Recém-Nascidos , Núcleo Celular/ultraestrutura , Cóclea/citologia , Cóclea/crescimento & desenvolvimento , Ducto Coclear/citologia , Ducto Coclear/crescimento & desenvolvimento , Citoplasma/ultraestrutura , Grânulos Citoplasmáticos/ultraestrutura , Matriz Extracelular/ultraestrutura , Heterocromatina/ultraestrutura , Junções Intercelulares/ultraestrutura , Microscopia Eletrônica , Mitocôndrias/ultraestrutura , Organelas/ultraestrutura , Ratos , Estria Vascular/citologia , Vacúolos/ultraestruturaRESUMO
As the cochlea develops, the cells in the basal cochlea become sensitive to progressively higher frequencies. To identify features of cochlear morphology that may underlie the place code shift, measurements of infant and adult gerbil cochleas were made at both the light and electron microscopic levels. The measurements included areas of the cochlear duct, basilar membrane, and organ of Corti, height and width of the basilar membrane, thickness of the tympanic cover layer, thickness of the upper and lower basilar membrane fiber bands, and optical density of the basilar membrane. The results indicated that basilar membrane dimensions do not change as the place code shifts and that regions that code for the roughly the same frequency (e.g., approximately 11.2 kHz) at different ages can have basilar membranes of very different dimensions. In contrast, the size of the organ of Corti and the thickness of fiber bands inside the basilar membrane do change in ways consistent with the shift in the frequency map.
Assuntos
Membrana Basilar/anatomia & histologia , Ducto Coclear/anatomia & histologia , Órgão Espiral/anatomia & histologia , Membrana Timpânica/anatomia & histologia , Estimulação Acústica , Análise de Variância , Animais , Membrana Basilar/fisiologia , Membrana Basilar/ultraestrutura , Ducto Coclear/fisiologia , Ducto Coclear/ultraestrutura , Gerbillinae , Microscopia Eletrônica , Órgão Espiral/fisiologia , Órgão Espiral/ultraestrutura , Membrana Timpânica/fisiologia , Membrana Timpânica/ultraestruturaRESUMO
In order to establish how endolymph volume is regulated, it is essential to be able to measure endolymph volume or cross-sectional area in vivo. We have developed methods to accomplish this by injecting the volume marker ion hexafluoroarsenate (AsF6) into endolymph by iontophoresis. For an injection at a constant rate, the endolymph concentration is inversely dependent on the cross-sectional area of the scala into which injection occurred. Marker concentrations were monitored by inserting ion-selective microelectrodes into endolymph near the injection site. In a previous study we quantified the degree of hydrops in animals following ablation of the endolymphatic sac. In the present study we validated the technique by comparing the endolymphatic cross-sectional area measured in vivo with AsF6 with that measured by established histologic procedures. The correlation between the two measures was good, with a coefficient of .903, although the area measured histologically was a little lower than that measured in vivo.
Assuntos
Endolinfa/fisiologia , Saco Endolinfático/anatomia & histologia , Animais , Cóclea/citologia , Cóclea/fisiopatologia , Cóclea/ultraestrutura , Ducto Coclear/citologia , Ducto Coclear/ultraestrutura , Hidropisia Endolinfática/diagnóstico , Hidropisia Endolinfática/patologia , Saco Endolinfático/patologia , Saco Endolinfático/ultraestrutura , Potenciais Evocados Auditivos , Cobaias , Doença de Meniere/complicações , Doença de Meniere/patologia , Fatores de TempoRESUMO
The differentiation of the rat stria vascularis (SV) was investigated by light- and electron microscopy and by immunocytochemistry. Loss of the basal lamina at the epithelial-mesenchymal interface of SVs as indicated by immunoreactions of laminin and fibronectin induces the formation of vascular feet by basal infoldings of the marginal cells (MCs), and the development of the strial capillaries (SCs) by mesenchymal cells in a manner of vasculogenesis is progressing at the same time. The production of fibronectin in the rough endoplasmic reticulum of mesenchymal cells and the involvement of this glycoprotein in a mechanical linkage between the vasoformative mesenchymal cells and endothelial ones of the SCs are indicated by immunocytochemistry. The plasma membrane of the marginal cells (MCs) begins to show immunoreactions of Na+.K+ ATPase at postnatal day 5 and is conjugated to each other by tight junctions at postnatal day 14. The apical tubules of the differentiating MCs do not seem to be involved in the endocytotic activity but are involved in the plasma membrane supply for the rapid differentiation.
Assuntos
Estria Vascular/citologia , Animais , Diferenciação Celular , Membrana Celular/enzimologia , Ducto Coclear/citologia , Ducto Coclear/embriologia , Ducto Coclear/ultraestrutura , Retículo Endoplasmático/ultraestrutura , Fibronectinas/metabolismo , Imuno-Histoquímica , Laminina/metabolismo , Mesoderma/citologia , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Microscopia Imunoeletrônica , Ratos , Ratos Wistar , ATPase Trocadora de Sódio-Potássio/metabolismo , Estria Vascular/ultraestruturaRESUMO
We demonstrated anionic sites on the lateral wall of cochlear duct and Reissner's membrane (RM) of ICR mice by Lowicryl K4M resin post-embedding and poly-L-lysine-colloidal gold conjugate (PL-CG) as a polycationic probe. The basement membrane and endolymphatic cell surface of RM were labeled with PL-CG pH 2.5 and pH 1.0. However, the perilymphatic cell surface was not labeled. PL-CG pH 2.5 and pH 1.0 strongly labeled the endolymphatic surface of the spiral prominence epithelium (SP), whereas the endolymphatic surface of the marginal cell (MC) in the stria vascularis was not labeled. Pre-digestion with several glycosidases eliminated PL-CG labeling. Our result suggests that an anionic charge located on the basement membrane of RM is largely due to the presence of heparan sulfate, chondroitin sulfate, and hyaluronic acid. An anionic charge on the endolymphatic cell surface of RM was mainly dependent on the presence of heparan sulfate. An anionic charge on the SP epithelium was caused to a substantial degree by chondroitin sulfate. We obtained histochemical evidence that the glycoconjugate content of the MC surface was quite different from that of the endolymphatic cell surface of RM and SP. We also identified RM-MC and SP-MC junctions at the ends of the stria vascularis between the marginal cells and the other endolymphatic epithelial cells of the cochlear duct.
Assuntos
Ducto Coclear/ultraestrutura , Estria Vascular/ultraestrutura , Resinas Acrílicas , Animais , Ânions , Membrana Basal/ultraestrutura , Membrana Celular/ultraestrutura , Sulfatos de Condroitina/análise , Condroitinases e Condroitina Liases/metabolismo , Ouro , Heparitina Sulfato/análise , Ácido Hialurônico/análise , Hialuronoglucosaminidase/metabolismo , Sistema Linfático/ultraestrutura , Masculino , Camundongos , Camundongos Endogâmicos ICR , Neuraminidase/metabolismo , Especificidade de Órgãos , Polilisina , Polissacarídeo-Liases/metabolismoRESUMO
In order to measure the mechanical strength of small and delicate biological specimens, a microtesting system was developed. We measured the mechanical properties of the round window, basilar and Reissner's membranes taken from the autopsied cases. The testing method was a displacement of the specimen by a force sensor needle until rupture. A load-displacement curve was then drawn and the mechanical value of each specimen was calculated. Reissner's membrane was the weakest, though it showed a measurable strength. From the load-displacement curve of the basilar membrane, it could be displaced by a smaller force than the rupture strength of Reissner's membrane. Speculation about the concept of a negative summating potential in Meniere's disease is therefore theoretically possible from a mechanical point of view.
Assuntos
Membrana Basilar/fisiologia , Ducto Coclear/fisiologia , Janela da Cóclea/fisiologia , Idoso , Membrana Basilar/ultraestrutura , Fenômenos Biomecânicos , Cadáver , Ducto Coclear/ultraestrutura , Feminino , Humanos , Masculino , Membranas/fisiologia , Membranas/ultraestrutura , Microscopia Eletrônica de Varredura , Pessoa de Meia-Idade , Pressão , Janela da Cóclea/ultraestruturaRESUMO
The ultrastructure of Reissner's membrane in the rabbit is described following vascular perfusion-fixation of live, anesthetized and artificially respirated healthy animals. A new and improved technique of fixation is employed that includes a pressure feedback controlled peristaltic pump and an oxygen-carrying fixative. In ultrathin sections capillaries were observed between the two cell layers comprising Reissner's membrane. The mesothelial cells facing the scala vestibuli were connected by junctional complexes and neither pores nor discontinuities were observed in the cell layer. In the epithelial cells a well-developed tubulocisternal endoplasmic reticulum (TER) was noted. Computerized three-dimensional reconstruction documented the continuity of this TER, from prominent disc-shaped subsurface cisterns lining the luminal cell membrane to smaller subsurface cisterns lining the abluminal and lateral cell membranes, forming a transcellular canalicular pathway. The possible function of the TER in Reissner's membrane is discussed with reference to endolymph/perilymph homeostasis.