RESUMO
Plant-soil feedbacks contribute to vegetation dynamics by species-specific interactions between plants and soil biota. Variation in plant-soil feedbacks can be predicted by root traits, successional position, and plant nativeness. However, it is unknown whether closely related plant species develop more similar plant-soil feedbacks than more distantly related species. Where previous comparisons included plant species from distant phylogenetic positions, we studied plant-soil feedbacks of congeneric species. Using eight intra-continentally range-expanding and native Geranium species, we tested relations between phylogenetic distances, chemical and structural root traits, root microbiomes, and plant-soil feedbacks. We show that root chemistry and specific root length better predict bacterial and fungal community composition than phylogenetic distance. Negative plant-soil feedback strength correlates with root-feeding nematode numbers, whereas microbiome dissimilarity, nativeness, or phylogeny does not predict plant-soil feedbacks. We conclude that root microbiome variation among congeners is best explained by root traits, and that root-feeding nematode abundances predict plant-soil feedbacks.
Assuntos
Geranium/fisiologia , Herbivoria/fisiologia , Solo , Animais , Geranium/genética , Geranium/microbiologia , Microbiota , Nematoides/fisiologia , Filogenia , Raízes de Plantas/genética , Raízes de Plantas/microbiologia , Raízes de Plantas/fisiologia , RizosferaRESUMO
Arrays of blue (B, 400-500 nm) and red (R, 600-700 nm) light-emitting diodes (LEDs) used for plant growth applications make visual assessment of plants difficult compared to a broad (white, W) spectrum. Although W LEDs are sometimes used in horticultural lighting fixtures, little research has been published using them for sole-source lighting. We grew seedlings of begonia (Begonia ×semperflorens), geranium (Pelargonium ×horturum), petunia (Petunia ×hybrida), and snapdragon (Antirrhinum majus) at 20°C under six sole-source LED lighting treatments with a photosynthetic photon flux density (PPFD) of 160 µmolâm-2âs-1 using B (peak = 447 nm), green (G, peak = 531 nm), R (peak = 660 nm), and/or mint W (MW, peak = 558 nm) LEDs that emitted 15% B, 59% G, and 26% R plus 6 µmolâm-2âs-1 of far-red radiation. The lighting treatments (with percentage from each LED in subscript) were MW100, MW75R25, MW45R55, MW25R75, B15R85, and B20G40R40. At the transplant stage, total leaf area, and fresh and dry weight were similar among treatments in all species. Surprisingly, when petunia seedlings were grown longer (beyond the transplant stage) under sole-source lighting treatments, the primary stem elongated and had flower buds earlier under MW100 and MW75R25 compared to under B15R85. The color rendering index of MW75R25 and MW45R55 were 72, and 77, respectively, which was higher than those of other treatments, which were ≤64. While photosynthetic photon efficacy of B15R85 (2.25 µmolâJ-1) was higher than the W light treatments (1.51-2.13 µmolâJ-1), the dry weight gain per unit electric energy consumption (in gâkWh-1) of B15R85 was similar to those of MW25R75, MW45R55, and MW75R25 in three species. We conclude that compared to B+R radiation, W radiation had generally similar effects on seedling growth at the same PPFD with similar electric energy consumption, and improved the visual color quality of sole-source lighting.
Assuntos
Antirrhinum/crescimento & desenvolvimento , Begoniaceae/crescimento & desenvolvimento , Geranium/crescimento & desenvolvimento , Petunia/crescimento & desenvolvimento , Antirrhinum/fisiologia , Begoniaceae/fisiologia , Geranium/fisiologia , Luz , Iluminação , Petunia/fisiologia , Fótons , Fotossíntese , Desenvolvimento Vegetal , Plântula/crescimento & desenvolvimento , Plântula/fisiologiaRESUMO
PREMISE OF THE STUDY: Abiotic environmental factors are often considered to be important in the distribution and maintenance of variation in sexual systems in flowering plants. Associations between sexes and abiotic factors are well documented in dioecious systems, but much less is known about this relationship in other sexually polymorphic systems. Species that are highly variable in sexual expression and habitat distribution can provide insights into the role of abiotic factors in maintaining variation in sexual expression. METHODS: Focusing on a sexually polymorphic species, Geranium transversale, we measured sexual expression at both the flower and the plant level and examined vegetative and floral traits, pollen deposition, and reproductive success. We also tested for correlations between sexual expression and other traits and examined whether and how these traits covaried with abiotic environmental conditions. KEY RESULTS: We identified unique variation of sexual expression in G. transversale. There are four sexual morphs that display different combinations of the three flower types (pistillate, staminate, and perfect). Sexual morphs that are phenotypically more female (i.e., female and gynomonoecious morphs) are found in wetter and milder environments, and flower earlier than morphs that are more male (i.e., hermaphroditic and andromonoecious morphs). Additionally, floral organ size and reproductive success are influenced not only by the flower type but also by the sexual morph of the plant. CONCLUSIONS: Environmental conditions are likely to cause some of the variation in sexual expression found in G. transversale. Both genetic and ecological factors likely contribute to the maintenance of sexual variation in this species.
Assuntos
Flores/anatomia & histologia , Geranium/fisiologia , Sementes/crescimento & desenvolvimento , Flores/fisiologia , Polinização , Reprodução/fisiologia , SoloRESUMO
In gynodioecious plants, females are expected to produce more or better seeds than hermaphrodites in order to be maintained within the same population. Even though rarely measured, higher seed production can be achieved through differences in physiology. In this work, we measured sexual dimorphism in several physiological traits in the gynodioecious plant Geranium sylvaticum. Photosynthetic rate, stomatal conductance, transpiration rate, WUE and isotopic signatures were measured in plants growing in two habitats differing in light availability. Females have been reported to produce more seeds than hermaphrodites. However, we did not observe any significant difference in seed output between the sexes in these experimental populations. Similarly, the sexes did not differ in any physiological trait measured. Seed production was strongly limited by light availability. Likewise, differences between plants growing in full light versus low light were detected in most physiological parameters measured. Our results show that the sexes in G. sylvaticum do not show any evidence of sexual dimorphism in physiology, which concurred with a lack of sexual differences in seed output.
Assuntos
Geranium/fisiologia , Fotossíntese/fisiologia , Caracteres Sexuais , Flores/fisiologia , Geranium/metabolismo , Luz , Transpiração Vegetal/fisiologia , Reprodução/fisiologia , Sementes/crescimento & desenvolvimentoRESUMO
Amongst gynodioecious plant breeding systems, there can exist intermediate morphs with a reduction in their male function (i.e. reduced number of functional anthers). Along with this sexual trimorphism, plants can also show floral colour polymorphism. Such intricate mixtures of phenotypes within a species may have complex effects on floral rewards. Floral rewards are known to vary between sexually dimorphic species and to a lesser extent between colour morphs. However, the interactive effect of sexual trimorphism and colour polymorphism is unexplored. We measured nectar's sugar content in the sexually trimorphic Geranium sylvaticum, a gynodioecious plant with a light/dark floral polymorphism. We found that nectar reward differed across genders and colour morphs. Results were not however consistent within the three genders; dark female and hermaphrodite flowers had higher sugar content than light morphs, whereas intermediate flowers did not. As expected, females and hermaphrodites had different nectar reward, with intermediate morphs being midway between the other genders. In intermediates, the sugar content was not related to the number of functional stamens. We show for the first time the existence of sex-specific differences between flower gender and colour morphs in nectar rewards. Our results demonstrate the importance of considering multiple and conflicting selection pressures to explain rewards.
Assuntos
Flores/genética , Geranium/genética , Melhoramento Vegetal , Polinização/genética , Feminino , Geranium/fisiologia , Humanos , Fenótipo , Pigmentação/genética , Néctar de Plantas/genética , Polinização/fisiologia , Comportamento Sexual/fisiologiaRESUMO
Plants growing at high densities elongate their shoots to reach for light, a response known as the shade avoidance syndrome (SAS). Phytochrome-mediated detection of far-red light reflection from neighboring plants activates growth-promoting molecular pathways leading to SAS However, it is unknown how plants that complete their life cycle in the forest understory and are shade tolerant prevent SAS when exposed to shade. Here, we show how two wild Geranium species from different native light environments regulate contrasting responses to light quality cues. A comparative RNA sequencing approach unveiled the molecular underpinnings of their contrasting growth responses to far-red light enrichment. It also identified differential phytochrome control of plant immunity genes and confirmed that far-red enrichment indeed contrastingly affects resistance against Botrytis cinerea between the two species. Furthermore, we identify a number of candidate regulators of differential shade avoidance. Three of these, the receptor-like kinases FERONIA and THESEUS1 and the non-DNA binding bHLH protein KIDARI, are functionally validated in Arabidopsis thaliana through gene knockout and/or overexpression studies. We propose that these components may be associated with either showing or not showing shade avoidance responses.
Assuntos
Brotos de Planta/fisiologia , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/fisiologia , Arabidopsis/efeitos da radiação , Botrytis , Resistência à Doença/genética , Perfilação da Expressão Gênica , Regulação da Expressão Gênica de Plantas , Geranium/crescimento & desenvolvimento , Geranium/microbiologia , Geranium/fisiologia , Geranium/efeitos da radiação , Doenças das Plantas/imunologia , Doenças das Plantas/microbiologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Proteínas de Plantas/fisiologia , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/microbiologia , Brotos de Planta/efeitos da radiação , Análise de Sequência de RNA , Especificidade da Espécie , Estresse Fisiológico , TranscriptomaRESUMO
PREMISE OF THE STUDY: Sex lability (i.e., gender diphasy) in plants is classically linked to the larger resource needs associated with the female sexual function (i.e., seed production) compared to the male function (i.e., pollen production). Sex lability in response to the environment is extensively documented in dioecious species, but has been largely overlooked in gynodioecious plants. METHODS: Here, we tested whether environmental conditions induce sex lability in the gynodioecious Geranium sylvaticum. We conducted a transplantation experiment in the field where plants with different sex expression were reciprocally transplanted between high light and low light habitats. We measured plants' reproductive output and sex expression over four years. KEY RESULTS: Our results show that sex expression was labile over the study period. The light level at the destination habitat had a significant effect on sexual expression and reproductive output, because plants decreased their reproductive output when transplanted to the low light habitat. Transplantation origin did not affect any parameter measured. CONCLUSIONS: This study shows that sex expression in Geranium sylvaticum is labile and related to light availability. Sexually labile plants did not produce more seeds or pollen, and thus, there was no apparent fitness gain in sexually labile individuals. Sex lability in gynodioecious plants may be more common than previously believed because detection of sex lability necessitates data on the same individuals over time, which is rare in sexually dimorphic herbaceous plants.
Assuntos
Geranium/fisiologia , Ecossistema , Geranium/efeitos da radiação , Luz , Pólen/fisiologia , Pólen/efeitos da radiação , Reprodução , Sementes/fisiologia , Sementes/efeitos da radiaçãoRESUMO
Sex-differential plasticity (SDP) hypothesis suggests that since hermaphrodites gain fitness through both pollen and seed production they may have evolved a higher degree of plasticity in their reproductive strategy compared to females which achieve fitness only through seed production. SDP may explain the difference in seed production observed between sexes in gynodioecious species in response to resource (nutrients or water) availability. In harsh environments, hermaphrodites decrease seed production whereas females keep it relatively similar regardless of the environmental conditions. Light availability can be also a limiting resource and thus could theoretically affect differently female and hermaphrodite seed output even though this ecological factor has been largely overlooked. We tested whether the two sexes in the gynodioecious species Geranium sylvaticum differ in their tolerance to light limitation during seed maturation in the field. We used a fully factorial block experiment exposing female and hermaphrodite plants to two different light environments (control and shade) after their peak flowering period. Specifically, we measured fruit and seed production in response to decreased light availability and compared it between the sexes. Shading reduced the number of fruits and seeds produced, but the decrease was similar between the sexes. Furthermore, shading delayed seed production by three days in both sexes, but did not affect seed mass, seed P content, or the probability of re-flowering the following year. Our results give no evidence for reproductive SDP in response to light during seed maturation.
Assuntos
Geranium/fisiologia , Geranium/efeitos da radiação , Luz , Sementes/crescimento & desenvolvimento , Caracteres Sexuais , Geranium/crescimento & desenvolvimento , Reprodução/efeitos da radiação , Sementes/efeitos da radiaçãoRESUMO
BACKGROUND AND AIMS: Gynodioecy, the co-occurrence of female and hermaphroditic individuals, is thought to be an intermediate step between hermaphroditism and separate sexes, a major transition in flowering plants. Because retaining females in a population requires that they have increased seed fitness (to compensate for the lack of pollen fitness), factors that affect seed fitness are of great importance to the evolution of this mating system and have often been studied. However, factors negatively affecting female fitness are equally important and have been largely neglected. One such factor stems from female flowers being less attractive to insects than hermaphrodite flowers, thereby decreasing their relative fitness. METHODS: To test the severity and consequences of this type of pollinator discrimination in Geranium maculatum, experimental populations with the range of sex ratios observed in nature were created, ranging from 13 % to 42 % females. Pollinators were observed in order to measure the strength of discrimination, and pollen deposition and seed production of both sexes were measured to determine the fitness consequences of this discrimination. Additionally a comparison was made across the sex ratios to determine whether discrimination was frequency-dependent. KEY RESULTS: It was found that female flowers, on average, were visited at half of the rate of hermaphrodite flowers, which decreased their pollen receipt and seed production. Additionally, females were most discriminated against when rare, due to both changes in the pollinators' behaviour and a shift in pollinator composition. CONCLUSIONS: The results suggest that pollinator discrimination negatively affects females' relative fitness when they are rare. Thus, the initial spread of females in a population, the first step in the evolution of gynodioecy, may be made more difficult due to pollinator discrimination.
Assuntos
Abelhas/fisiologia , Geranium/fisiologia , Óvulo Vegetal/fisiologia , Polinização/fisiologia , Animais , Geranium/anatomia & histologia , Óvulo Vegetal/anatomia & histologia , Pólen/fisiologia , Reprodução , Razão de MasculinidadeRESUMO
In gynodioecious plants, females are predicted to produce more and/or better offspring than hermaphrodites in order to be maintained in the same population. In the field, the roots of both sexes are usually colonized by arbuscular mycorrhizal (AM) fungi. Transgenerational effects of mycorrhizal symbiosis are largely unknown, although theoretically expected. We examined the maternal and paternal effects of AM fungal symbiosis and host sex on seed production and posterior seedling performance in Geranium sylvaticum, a gynodioecious plant. We hand-pollinated cloned females and hermaphrodites in symbiosis with AM fungi or in nonmycorrhizal conditions and measured seed number and mass, and seedling survival and growth in a glasshouse experiment. Females produced more seeds than hermaphrodites, but the seeds did not germinate, survive or grow better. Mycorrhizal plants were larger, but did not produce more seeds than nonmycorrhizal plants. Transgenerational parental effects of AM fungi were verified in seedling performance. This is the first study to show transgenerational mycorrhiza-mediated parental effects in a gynodioecious species. Mycorrhizal symbiosis affects plant fitness mainly through female functions with enduring effects on the next generation.
Assuntos
Geranium/microbiologia , Geranium/fisiologia , Micorrizas/fisiologia , Simbiose/fisiologia , Germinação , Modelos Lineares , Folhas de Planta/anatomia & histologia , Plântula/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimentoRESUMO
BACKGROUND AND AIMS: Physical dormancy (PY)-break in some annual plant species is a two-step process controlled by two different temperature and/or moisture regimes. The thermal time model has been used to quantify PY-break in several species of Fabaceae, but not to describe stepwise PY-break. The primary aims of this study were to quantify the thermal requirement for sensitivity induction by developing a thermal time model and to propose a mechanism for stepwise PY-breaking in the winter annual Geranium carolinianum. METHODS: Seeds of G. carolinianum were stored under dry conditions at different constant and alternating temperatures to induce sensitivity (step I). Sensitivity induction was analysed based on the thermal time approach using the Gompertz function. The effect of temperature on step II was studied by incubating sensitive seeds at low temperatures. Scanning electron microscopy, penetrometer techniques, and different humidity levels and temperatures were used to explain the mechanism of stepwise PY-break. KEY RESULTS: The base temperature (Tb) for sensitivity induction was 17·2 °C and constant for all seed fractions of the population. Thermal time for sensitivity induction during step I in the PY-breaking process agreed with the three-parameter Gompertz model. Step II (PY-break) did not agree with the thermal time concept. Q10 values for the rate of sensitivity induction and PY-break were between 2·0 and 3·5 and between 0·02 and 0·1, respectively. The force required to separate the water gap palisade layer from the sub-palisade layer was significantly reduced after sensitivity induction. CONCLUSIONS: Step I and step II in PY-breaking of G. carolinianum are controlled by chemical and physical processes, respectively. This study indicates the feasibility of applying the developed thermal time model to predict or manipulate sensitivity induction in seeds with two-step PY-breaking processes. The model is the first and most detailed one yet developed for sensitivity induction in PY-break.
Assuntos
Geranium/fisiologia , Dormência de Plantas/fisiologia , Estações do Ano , Temperatura , Geranium/anatomia & histologia , Geranium/ultraestrutura , Modelos Biológicos , Sementes/anatomia & histologia , Sementes/fisiologia , Sementes/ultraestrutura , Fatores de Tempo , ÁguaRESUMO
In the present study, FTIR was used to analyze changes in chemical component contents and spectra characters of 3-hexulose-6-phosphate synthase/6-phosphate-3-hexuloisomerase (HPS/PHI) over-expressing transgenic and wild-type (WT) geraniums under formaldehyde (HCHO) stress to examine if FTIR could be a new method for identification of phenotypic differences between the transgenic plants with a photosynthetic HCHO-assimilation pathway and the WT plants. The WT and transgenic geranium plants were treated with 4 mmol x L(-1) HCHO for 0, 1, 2, 3 and 4 days, respectively. The comparison of FTIR spectral characteristics at different time points between the transgenic and WT plants indicated that the contents of carbohydrate, proteins and aliphatic compounds were significantly higher than those in the WT plants after 4 days of HCHO-treatment. This may be due to installation of the photosynthetic HCHO-assimilation pathway in the transgenic geranium, which enhanced its ability to metabolize and assimilate HCHO, thus allowed more HCHO to be fixed to 6-phosphate fructose, and then entered assimilation pathways for synthesis of a variety of intracellular components. The results suggest that FTIR can be a new method to identify the phenotypic differences between transgenic plants with a photosynthetic HCHO-assimilation pathway and WT plants.
Assuntos
Formaldeído/efeitos adversos , Geranium/fisiologia , Aldeído Liases/metabolismo , Aldose-Cetose Isomerases/metabolismo , Geranium/efeitos dos fármacos , Fotossíntese , Plantas Geneticamente Modificadas/efeitos dos fármacos , Plantas Geneticamente Modificadas/fisiologia , Espectroscopia de Infravermelho com Transformada de Fourier , Estresse FisiológicoRESUMO
BACKGROUND AND AIMS: Plants exhibit a variety of reproductive systems where unisexual (females or males) morphs coexist with hermaphrodites. The maintenance of dimorphic and polymorphic reproductive systems may be problematic. For example, to coexist with hermaphrodites the females of gynodioecious species have to compensate for the lack of male function. In our study species, Geranium sylvaticum, a perennial gynodioecious herb, the relative seed fitness advantage of females varies significantly between years within populations as well as among populations. Differences in reproductive investment between females and hermaphrodites may lead to differences in future survival, growth and reproductive success, i.e. to differential costs of reproduction. Since females of this species produce more seeds, higher costs of reproduction in females than in hermaphrodites were expected. Due to the higher costs of reproduction, the yearly variation in reproductive output of females might be more pronounced than that of hermaphrodites. METHODS: Using supplemental hand-pollination of females and hermaphrodites of G. sylvaticum we examined if increased reproductive output leads to differential costs of reproduction in terms of survival, probability of flowering, and seed production in the following year. KEY RESULTS: Experimentally increased reproductive output had differential effects on the reproduction of females and hermaphrodites. In hermaphrodites, the probability of flowering decreased significantly in the following year, whereas in females the costs were expressed in terms of decreased future seed production. CONCLUSIONS: When combining the probability of flowering and seed production per plant to estimate the multiplicative change in fitness, female plants showed a 56 % and hermaphrodites showed a 39 % decrease in fitness due to experimentally increased reproduction. Therefore, in total, female plants seem to be more sensitive to the cost of reproduction in terms of seed fitness than hermaphrodites.
Assuntos
Flores/fisiologia , Geranium/fisiologia , Organismos Hermafroditas/fisiologia , Sementes/fisiologia , ReproduçãoRESUMO
BACKGROUND AND AIMS: The 'hinged valve gap' has been previously identified as the initial site of water entry (i.e. water gap) in physically dormant (PY) seeds of Geranium carolinianum (Geraniaceae). However, neither the ontogeny of the hinged valve gap nor acquisition of PY by seeds of Geraniaceae has been studied previously. The aims of the present study were to investigate the physiological events related to acquisition of PY and the ontogeny of the hinged valve gap and seed coat of G. carolinianum. METHODS: Seeds of G. carolinianum were studied from the ovule stage until dispersal. The developmental stages of acquisition of germinability, physiological maturity and PY were determined by seed measurement, germination and imbibition experiments using intact seeds and isolated embryos of both fresh and slow-dried seeds. Ontogeny of the seed coat and water gap was studied using light microscopy. KEY RESULTS: Developing seeds achieved germinability, physiological maturity and PY on days 9, 14 and 20 after pollination (DAP), respectively. The critical moisture content of seeds on acquisition of PY was 11 %. Slow-drying caused the stage of acquisition of PY to shift from 20 to 13 DAP. Greater extent of cell division and differentiation at the micropyle, water gap and chalaza than at the rest of the seed coat resulted in particular anatomical features. Palisade and subpalisade cells of varying forms developed in these sites. A clear demarcation between the water gap and micropyle is not evident due to their close proximity. CONCLUSIONS: Acquisition of PY in seeds of G. carolinianum occurs after physiological maturity and is triggered by maturation drying. The micropyle and water gap cannot be considered as two separate entities, and thus it is more appropriate to consider them together as a 'micropyle--water-gap complex'.
Assuntos
Transporte Biológico/fisiologia , Geranium/fisiologia , Germinação/fisiologia , Dormência de Plantas/fisiologia , Sementes/fisiologia , Permeabilidade da Membrana Celular/fisiologia , Dessecação , Geranium/citologia , Geranium/embriologia , Geranium/crescimento & desenvolvimento , Óvulo Vegetal/fisiologia , Sementes/citologia , Sementes/crescimento & desenvolvimento , Fatores de Tempo , Água/metabolismoRESUMO
Both plant sex and arbuscular mycorrhizal (AM) symbiosis influence resource acquisition and allocation in plants, but the interaction between these two components is not well established. As the different plant sexes differ in their resource needs and allocation patterns, it is logical to presume that they might differ in their relationship with AM as well. We investigate whether the association with AM symbiosis is different according to the host plant sex in the gynodioecious Geranium sylvaticum, of which, besides female and hermaphrodite plants, intermediate plants are also recognized. Specifically, we examine the effects of two different AM fungi in plant mass allocation and phosphorus acquisition using a factorial greenhouse/common garden experiment. Cloned G. sylvaticum material was grown in symbiosis with AM fungi or in non-mycorrhizal condition. We evaluated both the symbiotic plant benefit in terms of plant mass and plant P content and the fungal benefit in terms of AM colonization intensity in the plant roots and spore production. Our results suggest that G. sylvaticum plants benefit from the symbiosis with both AM fungal species tested but that the benefits gained from the symbiosis depend on the sex of the plant and on the trait investigated. Hermaphrodites suffered most from the lack of AM symbiosis as the proportion of flowering plants was dramatically reduced by the absence of AM fungi. However, females and intermediates benefited from the symbiosis relatively more than hermaphrodites in terms of higher P acquisition. The two AM fungal species differed in the amount of resources accumulated, and the fungal benefit was also dependent on the sex of the host plant. This study provides the first evidence of sex-specific benefits from mycorrhizal symbiosis in a gynodioecious plant species.
Assuntos
Geranium/microbiologia , Geranium/fisiologia , Micorrizas/fisiologia , Flores/fisiologia , Fósforo/metabolismo , Raízes de Plantas/microbiologia , Raízes de Plantas/fisiologia , Simbiose/fisiologiaRESUMO
Temperate forest herbs with seeds exhibiting both a physical and a physiological dormancy mechanism are rare, and knowledge on the factors regulating germination of these species is fragmentary. The biennial Geranium robertianum L. grows mainly in temperate woodlands, but can also be found in exposed habitats. Seedlings of G. robertianum are known to emerge from spring until autumn, but little is known about the environmental factors regulating germination. In this study, phenology of seedling emergence and of physical dormancy loss was examined for seeds buried at shaded or sunny exposed locations. The role of temperature in regulating dormancy and germination was analysed by incubating seeds in temperature sequences simulating temperatures that seeds experience in nature. The results indicate that most seeds of G. robertianum buried in sunny conditions germinate immediately after physical dormancy loss in summer. Seeds buried in shaded conditions also lose physical dormancy mainly during summer, but remain physiologically dormant and do not germinate until late winter or early spring. Besides physical dormancy, seeds of G. robertianum also initially have a high level of physiological dormancy, which is reduced during dry storage. Physiological dormancy is reduced through chilling in winter, thus enabling the seeds to germinate at low temperatures. We conclude that a complex combination of physical and physiological dormancy ensures that G. robertianum seeds germinate in summer at exposed sites and in early spring at shaded sites.
Assuntos
Geranium/fisiologia , Germinação , Estações do Ano , Plântula/crescimento & desenvolvimento , TemperaturaRESUMO
BACKGROUND AND AIMS: Physical dormancy in seeds of species of Geraniaceae is caused by a water-impermeable palisade layer in the outer integument of the seed coat and a closed chalaza. The chalazal cleft has been reported to be the water gap (i.e. location of initial water entry) in innately permeable seeds of Geraniaceae. The primary aim of this study was to re-evaluate the location of the water gap and to characterize its morphology and anatomy in physically dormant seeds of Geraniaceae, with particular reference to G. carolinianum. METHODS: Length, width, mass, anatomy and germination of two seed types (light brown and dark brown) of G. carolinianum were compared. Location, anatomy and morphology of the water gap were characterized using free-hand and microtome tissue sectioning, light microscopy, scanning electron microscopy, dye tracking, blocking and seed-burial experiments. KEY RESULTS: Treatment with dry heat caused a colour change in the palisade cells adjacent to the micropyle. When placed in water, the 'hinged valve' (blister) erupted at the site of the colour change, exposing the water gap. The morphology and anatomy in the water-gap region differs from those of the rest of the seed coat. the morphology of the seed coat of the water-gap region is similar in G. carolinianum, G. columbinum, G. molle and G. pusillum and differs from that of the closely related species Erodium cicutarium. CONCLUSIONS: Dislodgment of swollen 'hinged valve' palisade cells adjacent to the micropyle caused the water gap to open in physically dormant seeds of G. carolinianum, and it was clear that initial water uptake takes place through this gap and not via the chalazal opening as previously reported. This water gap ('hinged valve gap') differs from water gaps previously described for other families in morphology, anatomy and location in the seed coat.
Assuntos
Evolução Biológica , Transporte Biológico/fisiologia , Geraniaceae/fisiologia , Sementes/fisiologia , Água , Permeabilidade da Membrana Celular/fisiologia , Corantes , Geraniaceae/classificação , Geranium/classificação , Geranium/fisiologia , Germinação/fisiologia , Microscopia Eletrônica de Varredura/métodos , Modelos Biológicos , Permeabilidade , Filogenia , Especificidade da Espécie , Temperatura , Fatores de TempoRESUMO
Populations containing both females and hermaphrodites (dimorphic) are generally found in drier sites than those with only hermaphrodites (monomorphic). The sex-differential plasticity hypothesis (SDP) suggests that this is caused by hermaphrodites reducing allocation to seeds in harsh environments, allowing female establishment. We proposed that a similar process could explain sex distribution within populations. We compared light availability and soil moisture between sites of three monomorphic and three dimorphic populations of Geranium maculatum and between microsites occupied by females and hermaphrodites within populations. We also correlated seed production in dimorphic populations with environmental measures. We found that dimorphic and monomorphic populations occurred in sites with similar soil moisture but within two dimorphic populations females occurred in drier microsites than hermaphrodites, as predicted by the SDP hypothesis. Contrary to the predictions, hermaphrodites' seed production was not influenced by the environment. Rather, females' seed production was correlated with environmental conditions in two populations, although the direction of the correlation differed between populations. Our results suggest that in this species, the SDP hypothesis does not explain sex distribution among or within populations. However, microsite environments may influence the distribution of sexes within a population and potentially aid in maintaining gynodioecy.
Assuntos
Meio Ambiente , Geranium/fisiologia , Caracteres Sexuais , Flores/fisiologia , Luz , Modelos Biológicos , Dinâmica Populacional , Análise de Regressão , Estações do Ano , Sementes/crescimento & desenvolvimento , SoloRESUMO
BACKGROUND AND AIMS: For the maintenance of gynodioecy (i.e. the coexistence of female and hermaphroditic plants), females need to compensate for the lack of pollen production through higher seed production or better progeny quality compared to hermaphrodites. In Geranium sylvaticum, females produce more seeds per flower than hermaphrodites. This difference in seed production might be modified by biological interactions with pollinators and herbivores that may favour one sex and thus affect the maintenance of gynodioecy. METHODS: Sexual dimorphism in flower size and flowering phenology, and in attractiveness to pollinators, pre-dispersal seed predators and floral herbivores were examined in natural populations of G. sylvaticum. KEY RESULTS: Pollinators preferred hermaphrodites 25 % more often than females in two of the three study populations, and floral herbivores attacked hermaphrodites 15 % more often than females in two of the six study populations. These preferences might be explained by the larger flower size of hermaphrodites. In contrast, seed predators did not prefer either sex. CONCLUSIONS: The data suggest that pollinator preference does not benefit females, whereas the higher floral herbivory of hermaphrodites might enhance the maintenance of females in G. sylvaticum. Thus, although the data support the view that ecological factors may contribute to the maintenance of gynodioecy, they also suggest that these contributions may vary across populations and that they may function in opposite directions.
Assuntos
Flores/fisiologia , Geranium/fisiologia , Pólen/fisiologia , Adaptação Fisiológica , Análise de Variância , Reprodução , Sementes/fisiologia , Caracteres SexuaisRESUMO
Cytogenetic study of six cultivars and six selection lines of geranium (Pelargonium spp.), as well as of 100 plants regenerated from callus cultures has been performed. The majority of cultivars and lines had somatic chromosome numbers 2n = 7x = 56. Among regenerated plants of different cultivars (Rozovaja, Dushistaja, Krunk, Aist, Regar) obtained in vitro from various types of explants (internode, petiole) 61% of diploids and 39% aneuploids were revealed. Chromosome numbers in aneuploids varied from 46 to 82, among them 25.6% regenerated plants had 2n = 72; 10.2% -2n = 68; 5.1% -2n = = 64 and 12.8% -2n = 62. Addition of colhicine to nutrient medium increased the number of aneuploid plants.