Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.092
Filtrar
1.
Medicine (Baltimore) ; 99(17): e19959, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32332681

RESUMO

This study aimed to investigate the association between Serum Uric Acid (UA) to Creatinine (Cr) Ratio (UA/Cr) and metabolic syndrome (MetS) in postmenopausal women.A total of 455 patients with MetS and 457 age- and gender- matched controls were included in the present retrospective study. Serum levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting plasma glucose (FPG), Cr, and UA were measured. We employed logistic regression analysis to investigate the association between serum UA/Cr and MetS in postmenopausal women.Serum UA/Cr levels were significantly higher in patients with MetS than that in control subjects (P < .05). In the correlation analysis, serum UA/Cr showed a significantly positive correlation with age, hypertension, systolic pressure (SBP), diastolic pressure (DBP), Waist, body mass index (BMI), TG, UA and negative correlation with type 2 diabetes mellitus (T2DM) and Cr (P all < .001). Moreover, multivariate analysis revealed that serum UA/Cr was still an independent risk factor for MetS (OR = 2.928, 95% CI = 2.385-3.596, P < .001) after adjustments for other confounders.Serum UA/Cr are strongly associated with the risk of MetS in postmenopausal Chinese women.


Assuntos
Creatinina/análise , Síndrome Metabólica/sangue , Ácido Úrico/análise , Idoso , Índice de Massa Corporal , China , Creatinina/sangue , Feminino , Humanos , Modelos Logísticos , Masculino , Síndrome Metabólica/induzido quimicamente , Síndrome Metabólica/fisiopatologia , Pessoa de Meia-Idade , Pós-Menopausa/sangue , Estudos Retrospectivos , Fatores de Risco , Ácido Úrico/sangue , Circunferência da Cintura
2.
Medicine (Baltimore) ; 99(9): e19418, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32118796

RESUMO

Few studies on the individual and combined analysis between serum uric acid (SUA) and body mass index (BMI) and blood pressure (BP) were conducted in individuals aged ≥45 years. We aimed to assess the extent to which BMI and SUA and their interaction affect BP in Chinese middle-aged and older adults.Data were selected from the China Health and Retirement Longitudinal Study (CHARLS). A total of 5888 individuals aged 45 to 96 was included. Differences between BMI, or between categories of blood pressure were evaluated by t test or chi-square test. The trend of related variables according to four BMI categories was also tested using contrast analysis. The adjusted associations between various characteristics and BP status were first compared using linear regression models, as appropriate. Then, general linear models adjusting for related potential confounders were used to examine the synergistic effect of SUA and BMI level on BP for middle-aged and elderly individuals in China.Age-adjusted partial Pearson correlation coefficient showed that BMI was significantly and positively correlated with BP both in male and female, SUA positively correlated with both systolic blood pressure (SBP) and diastolic blood pressure (DBP) in males with BMI <24.0 kg/m and females with BMI <24.0 kg/m. However, SUA level significantly and positively correlated with DBP, but not with SBP, in females with BMI ≥24.0 kg/m. Multiple linear regression analysis showed that BMI was independently associated with BP both in male and female, SUA significantly and positively associated with SBP in both males and females with BMI <24.0 kg/m, However, SUA level positively correlated with DBP in females with BMI <24.0 kg/m, but not with males with BMI <24.0 kg/m, independent of other confounding factors. A general linear model analysis adjusted for confounding factors did not reveal interaction between BMI, SUA levels and SBP (ß=-1.404, P = .686 in males; ß=-2.583, P = .575 in females) and DBP (ß=-2.544, P = .263 in males; ß=-2.619, P = .622 in females).No interaction between BMI, SUA levels, and BP was observed in either males or females; However, BMI was independently associated with BP both in male and female, SUA independently associated with SBP both in males and females with BMI <24.0 kg/m, and SUA independently associated with DBP in females with BMI ≥24.0 kg/m.


Assuntos
Pressão Sanguínea/efeitos dos fármacos , Índice de Massa Corporal , Ácido Úrico/análise , Adulto , Idoso , Determinação da Pressão Arterial/métodos , China , Feminino , Humanos , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade
3.
Anal Bioanal Chem ; 412(3): 669-680, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31834450

RESUMO

A layer-by-layer self-assembled nitrogen-doped graphene/PDDA/gold nanoparticle (NDG/PDDA/GNP) composite was described. Citrate-stabilizing gold nanoparticle colloids (GNPs) were electrostatically adsorbed onto NDG nanosheets using a cationic polyelectrolyte, polydiallyldimethylammonium (PDDA), as the linker, thereby creating a high-performance electrochemical interface. The morphology and chemical composition were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, ultraviolet-visible spectroscopy, infrared spectroscopy, and Raman spectroscopy. Analytical application was manifested by electrochemical sensing of uric acid (UA), a biomarker involved with a variety of clinical diseases. The prepared nanocomposite exhibited noticeable electroactivity to uric acid oxidation and can give effective peak separation with ascorbic acid and dopamine. Additionally, the nanocomposite practically averted from other potentially interferents including glucose, urea, and serotonin, thus allowing selective voltammetric detection of UA in the biological matrix. Under the optimal condition, peak currents measured by differential pulse voltammetry were proportional to UA concentrations in the range of 0.5~100 µM (R2 = 0.998), with the detection limit of 53 nM. The NDG/PDDA/GNP nanocomposite as presented herein holds potential for aiding the diagnosis of UA-associated diseases and should be a new opportunity for biochemical analysis and biosensing applications. Graphical abstract.


Assuntos
Técnicas Eletroquímicas/métodos , Ouro/química , Grafite/química , Nanopartículas Metálicas/química , Nitrogênio/química , Polietilenos/química , Compostos de Amônio Quaternário/química , Eletricidade Estática , Ácido Úrico/análise , Limite de Detecção
4.
Int Braz J Urol ; 46(1): 70-80, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31851461

RESUMO

OBJECTIVE: To analyze the compositions of upper urinary tract stones and investigate their distributions in different gender and age groups. MATERIALS AND METHODS: Patients diagnosed with upper urinary tract stone disease between December 2014 and March 2018 were retrospectively reviewed. Patient's age, gender, BMI, comorbidities, stone event characteristics, and compositions were collected, and proportions of stone components in different gender and age groups were analyzed. RESULTS: A total of 1532 stone analyses were performed (992 from males and 540 from females). The mean age was younger in males (p<0.001). Males included more cases with larger BMI, hyperuricemia, and obesity, while females had more urinary tract infections. Multiple components were present in 61.8% of stones. Calcium oxalate (CaOx) (67.0%) was the most common component, followed by uric acid (UA) (11.8%), infection stone (11.4%), calcium phosphate (CaP) (8.0%), cystine (1.1%), brushite (0.4%), and 2,8-dihydroxyadenine (0.2%). Men contributed with more CaOx stones than women at age 30-49 years (all p<0.01) and more UA stones at 30-59 years (all p<0.05). Women contributed with more infection stones than men in age groups 30-49 and 60-69 years (all p<0.05), and more CaP stones at 30-49 years. The prevalence peak was 50-59 years in men and 60-69 years in women. Both genders had the lowest prevalence in adolescence. Prevalence of UA stones increased while that of infection stones decreased with aging in both genders. CONCLUSIONS: Age and sex had a strong association with distribution of stone compositions in this Chinese cohort.


Assuntos
Cálculos Urinários/química , Cálculos Urinários/epidemiologia , Adenina/análogos & derivados , Adenina/análise , Adulto , Distribuição por Idade , Fatores Etários , Oxalato de Cálcio/análise , Fosfatos de Cálcio/análise , China/epidemiologia , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Prevalência , Estudos Retrospectivos , Fatores de Risco , Distribuição por Sexo , Fatores Sexuais , Ácido Úrico/análise , Cálculos Urinários/etiologia
5.
Eur Radiol ; 30(1): 404-412, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31332560

RESUMO

OBJECTIVES: To develop a method that allows approximating the mass of monosodium uric acid (MSU) in a gouty tophus using phantom measurements and including tophus density into the calculation of the dual-energy computed tomography (DECT) tophus volumetry. METHODS: We prepared specimens of different concentrations of MSU placed in an epoxy-based phantom and an excised porcine foreleg. Density and volumetric measurements were performed in sequential single-source DECT scans acquired with increasingly higher tube currents. We developed a method for estimating the tophus mass by multiplying the detected tophus volume with its mean density and adding a specific gravimetric coefficient k. k was derived from the DECT scans by comparing the approximated MSU masses in the epoxy phantom with the known true MSU masses of the specimens. RESULTS: Comparison of the approximated MSU masses in the porcine foreleg scans with the true MSU masses of the syringe contents showed similar performance to sole volume measurement while providing additional information on the true uric acid burden: Over 70% of the true urate masses have been detected in MSU concentrations ≥ 45%, while the detection rate was much lower for MSU concentrations ≤ 40%. Retrospective analysis of patients with proven gouty arthritis confirmed the diagnostic potential of the mass approximation technique. CONCLUSIONS: We successfully established a method to include tophus density measurement for estimation of the uric acid burden in milligrams (instead of ml) in a phantom setting for MSU concentrations above 40%. Future studies should validate its use for follow-up in clinical practice. KEY POINTS: • Including tophus density measurement in dual-energy computed tomography scans in a phantom setting can be used for estimating the urate burden in milligrams, which might be useful for imaging follow-up. • The mass [mg] of the uric acid burden in a patient with gouty arthritis can be calculated by multiplying volume [ml] with mean density [HU] using a specific gravimetric coefficient. • Retrospective analysis of two patients with gouty tophi showed the relevance of measuring urate mass in addition to urate volume alone.


Assuntos
Artrite Gotosa/diagnóstico por imagem , Imagem Radiográfica a Partir de Emissão de Duplo Fóton/métodos , Tomografia Computadorizada por Raios X/métodos , Ácido Úrico/análise , Algoritmos , Animais , Artrite Gotosa/metabolismo , Humanos , Masculino , Imagens de Fantasmas , Estudos Retrospectivos , Suínos
6.
Vet Clin North Am Exot Anim Pract ; 23(1): 103-114, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31759442

RESUMO

Reptile kidneys maintain a constant extracellular environment within the body. They excrete waste products, maintain normal concentrations of salt and water, regulate acid-base balance, and produce hormones and vitamins. The kidneys contain nephrons consisting of glomeruli designed to filter the plasma, Bowman capsules that collect the filtrate, and tubules that resorb most of the filtered water and nutrients while excreting waste metabolites. A Loop of Henle is absent. Therefore, reptile kidneys cannot produce a hypertonic urine. The urinary bladder (if present) and cloaca excrete and absorb additional fluids and electrolytes. A renal portal system is present in all reptiles.


Assuntos
Répteis/fisiologia , Animais , Rim/metabolismo , Répteis/anatomia & histologia , Ureia/análise , Ácido Úrico/análise , Bexiga Urinária/anatomia & histologia , Bexiga Urinária/fisiologia
7.
Vet Clin North Am Exot Anim Pract ; 23(1): 151-168, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31759445

RESUMO

Renal disease is one of the most common medical conditions encountered in captive reptiles. In most cases, signs of disease are nonspecific and often not present until the condition is advanced. Many factors contribute to the development of renal disease, and the etiology often is multifactorial. Diagnosis of renal disease by traditional methods used in small animals is not as straightforward as in reptiles; often many tests may be needed to reach a firm diagnosis. Prevention is preferred to treatment. Understanding the pathophysiology, potential causes, diagnostic tests available, and treatment options is essential for the reptile veterinarian to manage this condition.


Assuntos
Gota/veterinária , Nefropatias/veterinária , Répteis , Animais , Gota/diagnóstico , Gota/fisiopatologia , Gota/terapia , Rim/fisiopatologia , Nefropatias/diagnóstico , Nefropatias/fisiopatologia , Nefropatias/terapia , Ácido Úrico/análise
8.
Vet Clin North Am Exot Anim Pract ; 23(1): 47-58, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31759451

RESUMO

Renal disease often remains undetected in living patients. Urinalysis might contribute to the diagnosis of some kinds of renal and metabolic diseases. Blood uric acid concentrations reflect the excretory functional capacity of the renal proximal tubules. In contrast, blood urea concentrations are significantly affected by the bird's hydration status and have been proposed as a useful variable to detect prerenal causes for renal impairment in birds. Measurement of exogenous creatinine excretion shows promising preliminary results to become a useful test for the assessment of renal excretion in birds.


Assuntos
Doenças das Aves/fisiopatologia , Creatinina/análise , Nefropatias/veterinária , Animais , Doenças das Aves/diagnóstico , Aves , Creatinina/metabolismo , Rim/metabolismo , Nefropatias/diagnóstico , Nefropatias/fisiopatologia , Nefrologia , Ureia/análise , Ácido Úrico/análise , Urinálise/veterinária
9.
Talanta ; 207: 120296, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594571

RESUMO

Carbon materials and the metal nanocrystals enclosed by high-index facets have aroused considerable attention due to their large specific surface area, excellent electrical properties and catalysis activity, and easy accessibility for target molecules. In this study, the Pt concave nanocube (CNC) with high-index {410} and {510} facets was successfully prepared via a simple hydrothermal method. The multiwalled carbon nanotubes (MWCNT) and graphene oxide (GO) were chosen as the supporting materials to further improve the dispersion of Pt CNC and the electrical conductivity of nanocomposite. The nanocomposite was used to modify electrode for sensitive simultaneous detection dopamine and uric acid. The cyclic voltammetry and differential pulse voltammetry were applied to confirm the catalytic activity of proposed sensor. By using the as-synthesized nanomaterial as nonenzymatic sensing material, dopamine and uric acid can be detected with high sensitivity and selectivity. The linear range towards dopamine sensing is from 0.8 to 300 µM, and the limit of detection is 0.27 µM. For uric acid sensing, the sensor exhibited over two wide linear ranges (from 1 µM to 0.3 mM and from 0.3 mM to 1 mM) with a detection limit of 0.35 µM. The sensor also possessed a long-term stability, good reproducibility and a promising application for electrochemical detection of both dopamine and uric acid in real samples.


Assuntos
Dopamina/análise , Eletroquímica/instrumentação , Grafite/química , Limite de Detecção , Nanotubos de Carbono/química , Platina/química , Ácido Úrico/análise , Dopamina/química , Eletrodos , Modelos Moleculares , Conformação Molecular , Nanocompostos/química , Fatores de Tempo , Ácido Úrico/química
10.
Talanta ; 207: 120318, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31594597

RESUMO

This study designed a simplistic, efficient, and greener procedure to synthesize CeO2-CNTs. The analysis of structural and morphological characteristics of nano-composites has been done with regard to different procedures (e.g., EDX, XRD, & FESEM). In addition, simultaneous detection of ascorbic acid (AA), dopamine (DA), uric acid (UA) and acetaminophen (AC) has been examined at the modified glassy carbon electrode with CeO2-CNTs nano-composites. The surface area and electron transfer speed of the interplay between neuro-transmitters and electrode may be efficiently enhanced due to the existence of CeO2 nano-particles on CNTs surfaces. Moreover, electro-chemical behavior of electrodes has been dealt with by differential pulse voltammetry (DPV), impedance analysis (EIS), and cyclic voltammetry (CV). Acceptable linear response of AA, DA, UA and AC respectively have been ranged 0.01-900.0 µM, 0.01-700.0 µM, 0.01-900.0 µM, and 0.01-900.0 µM with determination limits (S/N = 3) of 3.1 nM, 2.6 nM, 2.4 nM and 4.4 nM. Ultimately, this procedure was used with successful results for determining AA, DA, UA and AC in real specimens, which suggested probable uses in other sensing studies.


Assuntos
Acetaminofen/análise , Ácido Ascórbico/análise , Cério/química , Dopamina/análise , Micro-Ondas , Nanotubos de Carbono/química , Ácido Úrico/análise , Acetaminofen/química , Ácido Ascórbico/química , Dopamina/química , Eletroquímica , Eletrodos , Química Verde , Concentração de Íons de Hidrogênio , Limite de Detecção , Nanopartículas/química , Nanotecnologia , Propriedades de Superfície , Fatores de Tempo , Ácido Úrico/química
11.
Urologiia ; (5): 31-36, 2019 Dec.
Artigo em Russo | MEDLINE | ID: mdl-31808629

RESUMO

THE AIM: To assess the diagnostic performance of dual-energy computed tomography (DECT) in the evaluation of the composition of urinary stones "in vivo". MATERIALS AND METHODS: A total of 91 patients aged from 20 to 70 years old (mean 42.7) with urinary stone disease were examined at Sechenov University, including 68 men (75%) and 23 women (25%). Prior to surgery, all patients underwent DECT (Canon, Japan) in order to predict the chemical composition of urinary stones in vivo. Extracorporeal shockwave lithotripsy (ESWL), percutaneous nephrolithotomy (PCNL) and ureteroscopy (URS) was performed in 53 (58.2%), 18 (19.7%) and 20 (22.1%) patients, respectively. Postoperatively, all stones or stone fragments (n=91; 100%) were examined using a comprehensive physical and chemical analysis (X-ray phase analysis, electron microscopy, infrared spectroscopy). RESULTS: In 6 patients (6.6%) staghorn stones were diagnosed, while in 15 (16.5%), 17 (18.7%), 22 (24.2%) and 31 (34.1%) stones were located in ureteropelvic junction, pelvis and ureter, respectively, including 24 patients with lower ureteric stones (26.4%). Prediction of the stone composition in vivo was carried out on the basis of the one indicator, the dual energy ratio (DER). The threshold values of DER for different types of stones were taken from the literature. All stones were divided into 4 groups according to the DECT results: vevellite stones (n=40, 43.9%), Ca-containing stones without vevellite (n=34, 37.3%), uric acid stones (n=10, 10.9%) and struvite stones (n=7, 7.9%). Thus, when comparing the results of DECT and physical and chemical analysis, in the first group four stones were incorrectly assigned by DECT to the group of Ca-containing stones without vevellite and three stones were incorrectly assigned to the group of struvite stones; in the second group four stones were incorrectly assigned to the group of vevellite stones; in the third group one stone was incorrectly assigned to the group of struvite stones; in the fourth group one stone was incorrectly assigned to the group of vevellite stones and one stone in the group of uric acid stones. In order to increase the diagnostic efficiency of DECT, we performed a comprehensive analysis of five specific DECT indicators (stone density at 135 kV, Z eff of the stone, DER, DEI, DED) using discriminant analysis. Thus, the sensitivity, specificity and overall accuracy of DECT with the use of just one indicator (DER) were 83.3%, 89.8%, 86.8% for vevellite, 88.2%, 92.9%, 91.2% for Ca-containing stones without vevellite, 90%, 98.8%, 97.8% for uric acid stones and 60%, 95.3%, 93.4% for struvite stones, respectively. When using discriminant analysis with five specific DECT indicators, higher values of sensitivity, specificity and overall accuracy were seen: 95.2%, 89.8%, 92.3% for a vevellite, 85,3%, 96,4%, 92,3% for Ca-containing stones without a vevellite and 100%, 100% and 100% for both uric acid and struvite stones, respectively. CONCLUSIONS: Dual-energy computed tomography is a highly informative method which allows to perform preoperatively the reliable assessment of the chemical composition. DECT in patients with urinary stone disease allows to optimize the treatment strategy and carry out preventive measures on individual basis, taking into account the stone type.


Assuntos
Cálcio/análise , Tomografia Computadorizada por Raios X/métodos , Ácido Úrico/análise , Cálculos Urinários/química , Cálculos Urinários/diagnóstico por imagem , Urolitíase/diagnóstico por imagem , Adulto , Idoso , Feminino , Humanos , Litotripsia , Masculino , Pessoa de Meia-Idade , Cálculos Ureterais , Adulto Jovem
12.
Turk J Ophthalmol ; 49(4): 178-182, 2019 09 03.
Artigo em Inglês | MEDLINE | ID: mdl-31486603

RESUMO

Objectives: To investigate corneal endothelial cell density (ECD), morphology, and central corneal thickness (CCT) in patients with gout compared with healthy subjects. Materials and Methods: Fifty eyes of 50 gout patients and 50 eyes of 50 healthy subjects without gout or any other systemic disease were included in this study. After detailed ophthalmologic examination, specular microscopy (Tomey EM-4000; Tomey Corp) measurement was performed for all participants. ECD, average cell area (ACA), coefficient of variation (CV), hexagonality ratio, and CCT values were recorded. Results: Mean ECD and hexagonality ratio were lower (p=0.004 and p=0.002) and CV, ACA, and CCT values were higher (p=0.001, p=0.007, and p=0.001) in patients with gout when compared to healthy subjects. There were significant correlations between gout disease duration and CD and hexagonality ratio (p=0.019 and p=0.043) and also between uric acid value and hexagonality ratio and CCT (p=0.044 and p=0.003). Conclusion: Altered corneal endothelial function was found in patients with gout when compared to healthy subjects and the alteration increased as gout duration and uric acid value increased.


Assuntos
Células Endoteliais/patologia , Epitélio Posterior/patologia , Gota/patologia , Adulto , Estudos de Casos e Controles , Contagem de Células , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Ácido Úrico/análise
13.
Fish Physiol Biochem ; 45(6): 1895-1905, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31399920

RESUMO

The present study investigates the nephrotoxic effects of two acute doses of silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on the African catfish, Clarias gariepinus, using biochemical, histochemical, and histopathological changes as biomarkers. AgNP-induced impacts were recorded in some of these characteristics on the bases of their size (20 and 40 nm) and concentration (10 and 100 µg/L) but no significant interaction between size and concentration. AgNO3 had low significant adverse effects on some parameters in comparison with those impacts of AgNPs. The concentrations of creatinine and uric acid exhibited different significant variations under stress in all exposed groups compared with those in the control group. On the tissue and cell levels, histopathological changes were observed. These changes include hypertrophies of glomeruli, proliferation in the haemopoietic tissue, dissociation in renal tubules, shrinkage of glomerulus, hydropic degeneration, dilatation of renal tubules, aggregation of melanomacrophages, rupture of Bowman's capsule, and the glomerular tuft and dilatation of Bowman's space. In more severe cases, the degenerative process leads to tissue necrosis in the kidney of AgNP-exposed fish as well as carbohydrate depletion; a faint coloration was also observed in the brush borders and basement membrane with a large amount of connective tissue fibers around the blood vessels and the renal tubules. Recovery period for 15 days led to improvement of most of the alterations in biochemical, histopathological, and histochemical parameters induced by AgNPs and AgNO3. In conclusion, one can postulate on the sensitivity of the kidney of C. gariepinus to AgNPs and recovery strategy is a must.


Assuntos
Peixes-Gato , Rim/efeitos dos fármacos , Nanopartículas Metálicas/toxicidade , Prata/toxicidade , Animais , Creatinina/análise , Rim/patologia , Nitrato de Prata/toxicidade , Ácido Úrico/análise
14.
Med Sci Monit ; 25: 5191-5200, 2019 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-31300636

RESUMO

BACKGROUND Aspirin hyporesponsiveness increases the risk of ischemic events. Therefore, it is important to investigate the factors influencing aspirin hyporesponsiveness. MATERIAL AND METHODS Patients aged 60 years or older who did not take aspirin before enrollment were included, with aspirin 100 mg/day administered after enrollment. The arachidonic acid-induced platelet aggregation rate (Ara) was measured by light transmission assay to evaluate aspirin responsiveness. Patients with Ara in the upper quartile after taking aspirin were assigned to the aspirin hyporesponsive group (Ara-Q4). RESULTS A total of 292 elderly patients were included. The median value of Ara after taking aspirin was 5.87% (interquartile range 3.86-10.04%). Compared with the aspirin non-hyporesponsive group (Ara-Q1-3, Ara ≤10.04%, n=220), the level of uric acid (UA) (341.30 µmol/L vs. 299.10 µmol/L, p=0.027) and the ratios of ß-blockers (9.72% vs. 2.27%, p=0.015) and diuretics (6.94% vs. 1.36%, p=0.036) were higher in the aspirin hyporesponsive group (Ara-Q4, Ara >10.04%, n=72). After multivariate adjustment, the results demonstrated baseline Ara (odds ratio [OR]: 1.030, 95% confidence interval [CI]: 1.004-1.056, p=0.021), UA level (OR: 1.003, 95% CI: 1.000-1.006, p=0.038), and ß-blockers use (OR: 5.487, 95% CI: 1.515-19.870, p=0.010) were independently and positively associated with aspirin hyporesponsiveness. CONCLUSIONS This study found that baseline Ara, UA level, and ß-blockers use were independently and positively associated with aspirin hyporesponsiveness in elderly Chinese patients, which needs to be validated in large-scale studies.


Assuntos
Aspirina/administração & dosagem , Agregação Plaquetária/efeitos dos fármacos , Antagonistas Adrenérgicos beta/administração & dosagem , Idoso , Idoso de 80 Anos ou mais , Grupo com Ancestrais do Continente Asiático , Biomarcadores Farmacológicos/metabolismo , China , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Inibidores da Agregação de Plaquetas/farmacologia , Testes de Função Plaquetária , Ácido Úrico/análise
15.
Diabetes Res Clin Pract ; 155: 107781, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31279959

RESUMO

AIMS: To analyze the factors associated with non-albuminuric reduced estimated glomerular filtration rate (NAeGFR-) phenotype in young people with type 1 diabetes (T1DM). METHODS: In this cross-sectional study were enrolled 140 outpatient diabetic children (age 7-18 years), consecutively observed in the period 2016-2017. Eighteen subjects with microalbuminuria (defined as albumin excretion rate ≥ 30 mg/24 h) were excluded. Fasting HbA1c, uric acid (UA), neutrophils and lymphocytes count were recorded. Estimated glomerular filtration rate (eGFR) was calculated using the Schwartz's bed-side formula and reduced eGFR was defined by a value <90 mL/min/1.73 m2. RESULTS: Out of 122 subjects analyzed, 76 (62%) showed normal eGFR and 46 (38%) showed NAeGFR- phenotype. They were characterized by higher prevalence of male sex (57% vs 33%, p = 0.010), autoimmune diseases (26% vs 12%, p = 0.043), high UA levels (4.0 ±â€¯0.9 vs 3.3 ±â€¯0.9 mg/dl, p < 0.0001) and high Neutrophils/Lymphocytes ratio (1.5 [1.2-2.0] vs 1.3 [1.0-1.8], p = 0.023). CONCLUSIONS: In our population, the prevalence on NAeGFR- phenotype is 38% and it is associated with male sex, high levels of UA, presence of other autoimmune diseases and low-grade inflammation. It should encourage pediatricians to monitor early both eGFR and UA in order to intercept diabetic youth more likely prone to develop progressive renal impairment.


Assuntos
Albuminas/análise , Diabetes Mellitus Tipo 1/fisiopatologia , Taxa de Filtração Glomerular , Insuficiência Renal/epidemiologia , Adolescente , Criança , Estudos Transversais , Diabetes Mellitus Tipo 1/metabolismo , Feminino , Hemoglobina A Glicada/análise , Humanos , Incidência , Itália/epidemiologia , Masculino , Fenótipo , Prevalência , Ácido Úrico/análise
16.
Anal Bioanal Chem ; 411(29): 7681-7688, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31286178

RESUMO

A new electrochemical sensor, based on NdFeO3 nanoparticles as electrocatalytic material, was proposed here for the detection of dopamine (DA). NdFeO3 nanoparticles were first synthesized by a simple thermal treatment method and subsequent annealing at high temperature (700 °C). The prepared electrocatalytic material has been characterized in detail by SEM-EDX, XRD, and Raman techniques. Characterization results display its sheet-like morphology, constituted by a porous network of very small orthorhombic NdFeO3 nanoparticles. NdFeO3 electrocatalytic material was then used to modify the working electrode of screen-printed carbon electrodes (SPCEs). Electrochemical tests demonstrated that NdFeO3- modified screen-printed carbon electrode (NdFeO3/SPCE) exhibited a remarkable enhancement of the dopamine electrooxidation, compared to the bare SPCE one. The analytical performance of the developed sensor has been evaluated for the detection of this analyte by means of the square-wave voltammetry (SWV) technique. The modified electrode showed two linear concentration ranges, from 0.5 to 100 µM and 150 to 400 µM, respectively, a limit of detection (LOD) of 0.27 µM (at S/N = 3), and good reproducibility, stability, and selectivity. Additionally, we also report an attempt made to propose the modified sensor for the simultaneous detection of dopamine and uric acid (UA). The procedure was also applied for the determination of dopamine in spiked real samples. So, this paper reports for the first time the use of a modified NdFeO3 screen-printed electrode for developing an electrochemical sensor for the quantification of important biomolecules. Graphical abstract.


Assuntos
Dopamina/análise , Técnicas Eletroquímicas/instrumentação , Compostos Férricos/química , Neodímio/química , Técnicas Biossensoriais , Catálise , Eletrodos , Humanos , Limite de Detecção , Reprodutibilidade dos Testes , Ácido Úrico/análise
17.
Dtsch Med Wochenschr ; 144(15): 1055-1060, 2019 08.
Artigo em Alemão | MEDLINE | ID: mdl-31350748

RESUMO

Crystals are one of the commonest reasons for acute joint inflammation. The most relevant types of crystals are those of monosodium urate (MSU) and calcium pyrophosphates (CPP). To get proven diagnosis of a crystal arthropathy the microscopic identification of those crystals in synovial fluid is still recommended by the actual guidelines. Whenever arthrocentesis is not feasible, ultrasound or dual-energy-computed tomography might help to visualize specific changes induced especially by MSU crystals. Both types of crystals act as danger signals inducing flares of immediate inflammatory response via activation of the innate immune system. Therefore crystal arthropathies could be seen as an auto-inflammatory condition. As neutrophils, monocytes and macrophages are the key cells and Interleukin 1ß is one of the dominant cytokines the way of blocking inflammation by colchicine and override IL-1ß are specific options in treating inflammation due to the crystals. For gout, causal treatment with urate lowering therapy can result in clearance of urate crystals. Unfortunately, to date there is no causal therapy for CPPD available. The present article summarises the recent knowledge highlighting the news regarding the crystal arthropathies gout and CPPD.


Assuntos
Artropatias por Cristais , Anti-Inflamatórios/uso terapêutico , Artropatias por Cristais/diagnóstico , Artropatias por Cristais/patologia , Artropatias por Cristais/terapia , Citocinas/sangue , Humanos , Microscopia , Ácido Úrico/análise
18.
Urology ; 131: 46-52, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31158354

RESUMO

OBJECTIVE: To determine if medical therapy affects long-term clinical outcomes in uric acid stone formers (UASF). METHODS: We identified 53 UASF who had complete stone clearance following stone procedure by computed tomography (CT) and had ≥1 postoperative 24-hour urine collection and a clinical follow-up ≥6 months with a surveillance CT scan. Patients were divided into "adherent to medical therapy" (compliance with potassium citrate ± allopurinol verified by computerized pharmacy data) or nonadherent groups. Primary outcomes were CT stone recurrence rate and need for surgical stone intervention. RESULTS: We found 28 of 53 (53%) adherent and 25 of 53 (47%) nonadherent individuals (14 declined medication, 11 intolerant). With median follow-up of 24 months, no significant differences were noted between groups in regards to stone recurrence (32%; P = .99) or in 24-hour urine pH compared to baseline or follow-up (range 5.46-5.62; P = 0.06). Adherent patients, however, had smaller CT stone recurrence sizes (6.3 ± 3.8 vs 11.8 ± 6.2 mm, P = .02), were 28% less likely to require stone surgery compared to those without therapy (P <.01), and trended toward longer time intervals without recurrence (23.1 ± 18.8 vs 10.5 ± 7.5 months, P = .10) compared to nonadherents. Study confounders included a variety of medication dosages and adherences, limited nonadherent follow-up, and small study number. CONCLUSION: UASF adherent to medical therapy had smaller recurrence sizes and fewer surgical interventions vs nonadherent, highlighting the protective role of potassium citrate in UA stone disease. The comparable urine pH and stone recurrence rates between groups, however, underscore areas for improvement in future UA stone prevention strategies.


Assuntos
Cálculos Renais/tratamento farmacológico , Idoso , Feminino , Humanos , Cálculos Renais/química , Masculino , Adesão à Medicação/estatística & dados numéricos , Pessoa de Meia-Idade , Recidiva , Estudos Retrospectivos , Fatores de Tempo , Resultado do Tratamento , Ácido Úrico/análise
19.
Food Chem ; 293: 23-31, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31151606

RESUMO

Cyclic voltammetry (CV) and high performance liquid chromatography (HPLC), were compared to quantify uric acid and antioxidant reducing agents in 36 milk samples. The enzymes uricase and ascorbate oxidase were used to remove uric and ascorbic acids and showed that the peaks obtained by CV and HPLC did not contain contributions from other unknown compounds. The levels of uric acid obtained by CV compared well to those determined using HPLC, with only a few exceptions, and the average difference was around 6%. CV measurements were made using the main anodic peak seen at approximately 330 mV (Ag/AgCl), while a later oxidation peak at approximately 650 mV can be associated with further reducing agents present in milk. The electrochemical method was quicker to apply than HPLC that included a pre-treatment step, and provides an inexpensive and simple method for the reliable analysis of uric acid in milk.


Assuntos
Técnicas Eletroquímicas , Leite/química , Substâncias Redutoras/análise , Ácido Úrico/análise , Animais , Antioxidantes/química , Ascorbato Oxidase/metabolismo , Cromatografia Líquida de Alta Pressão , Eletrodos , Concentração de Íons de Hidrogênio , Temperatura , Urato Oxidase/metabolismo
20.
Food Chem ; 293: 254-262, 2019 Sep 30.
Artigo em Inglês | MEDLINE | ID: mdl-31151609

RESUMO

This study reported a novel highly active alkaline urate oxidase (UOX) and demonstrated its application in reducing uric acid content of food under alkaline conditions. The UOX gene was cloned from Arxula adeninivorans NBRC 10858, and its N-terminally his6-tagged form (rUOX) was overexpressed in Escherichia coli. The rUOX displayed maximal activity at 40 °C and pH 10, kept more than 90% initial activity under alkaline conditions (pH 9-11) and more than 80% at temperatures below 55 °C. The apparent Km, turnover number (kcat) and catalytic efficiency (kcat/Km) values for the substrate uric acid were respective 29.15 µM, 151.16 s-1 and 5.19 s-1. µM-1, which are improvements over previously reported UOXs. The rUOX efficiently reduced uric acid and purine contents in beer, beef and yeast extract at pH 10, indicating a promising application in food with low purine and uric acid contents to prevent hyperuricemia and gout.


Assuntos
Escherichia coli/genética , Análise de Alimentos/métodos , Saccharomycetales/enzimologia , Urato Oxidase/metabolismo , Ácido Úrico/análise , Animais , Cerveja/análise , Catálise , Bovinos , Temperatura Alta , Concentração de Íons de Hidrogênio , Hiperuricemia/prevenção & controle , Carne/análise , Purinas/análise , Urato Oxidase/genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA