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1.
Rev. Odontol. Araçatuba (Impr.) ; 42(1): 9-13, jan.-abr. 2021. tab
Artigo em Português | LILACS, BBO - Odontologia | ID: biblio-1148159

RESUMO

O objetivo do presente estudo foi mensurar o pH externo radicular de dentes bovinos que foram desinfetados em solução de cloramina por 7 dias. Neste estudo foram utilizadas soluções irrigadoras, hipoclorito de sódio 1%, clorexidina 0,12% e hipoclorito de sódio 2,5% associadas ao edta e as medicações intracanais, hidróxido de cálcio, hidróxido de cálcio com PMCC e clorexidina gel 2%. O preparo químico mecânico foi realizado com as limas easy logic e as soluções foram agitadas com ultrassom durante 3 minutos e após os dentes foram imersos em água deionizada em eppendorfs estéreis e mantidos em estufa a 37°C. O pH externo foi analisado utilizando as fitas medidoras de pH no período de 24 horas, 48 horas, 7 dias, 10 dias e 15 dias. A normalidade dos valores obtidos de cada ensaio foi testada através do teste Kolmogorof- Smirnov, e o teste estatístico foi ANOVA de uma via e comparações múltiplas de Tukey. Os resultados mostraram que houve diferença estatística nos valores de pH nos grupos avaliados dentro dos tempos (p< 0,05). Concluiu-se que mesmo com as variações de pH nos períodos avaliados, as medicações e as soluções irrigantes podem ser utilizadas de forma associadas na endodontia, com o intuito de eliminar o maior número de microrganismos dos canais radiculares(AU)


The objective of the present study was to measure the external root pH of bovine teeth that were disinfected in chloramine solution for 7 days. Irrigating solutions, 1% sodium hypochlorite, 0.12% chlorhexidine and 2.5% sodium hypochlorite associated with edta and intracanal medications, calcium hydroxide, calcium hydroxide with PMCC and chlorhexidine gel 2%. The mechanical chemical preparation was performed with the easy logic files and the solutions were shaken with ultrasound for 3 minutes and after the teeth were immersed in deionized water in eppendorfs and kept in an oven at 37 ° C. The external pH was analyzed using the pH measuring tapes in the period of 24 hours, 48 hours, 7 days, 10 days and 15 days. The normal values obtained from each test were tested using the Kolmogorof-Smirnov test, and the statistical test was one-way ANOVA and Tukey's multiple comparisons. The results showed that there was a statistical difference in pH values in the groups evaluated within the times (p< 0.05). It was concluded that even with pH variations in the evaluated periods, medications and irrigating solutions can be used in an associated way in endodontics, in order to eliminate the largest number of microorganisms in the root canals(AU)


Assuntos
Irrigantes do Canal Radicular , Preparo de Canal Radicular , Hipoclorito de Sódio , Hidróxido de Cálcio , Cloraminas , Clorexidina , Análise de Variância , Ácido Edético , Endodontia
2.
J Adhes Dent ; 23(2): 167-175, 2021 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-33825430

RESUMO

PURPOSE: To investigate the effects of root canal irrigants, dentin surface treatment, and timing of the restoration on the microtensile bond strength (µTBS) of a universal adhesive to deep coronal dentin. MATERIALS AND METHODS: Ninety (90) intact molars were grouped according to the irrigation protocol: group 0 (saline, control group); group 1: 5.25% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid (EDTA) followed by 5.25% NaOCl; group 2: 18% etidronic acid (HEDP) + 5.25% NaOCl in a single mixture. Groups 1 and 2 were divided into 2 subgroups (n = = 10) according to the time of restoration: immediate (subgroup A) and after 7 days (subgroup B). Four other groups (n = 10) were created according to the dentin surface pretreatment and received an immediate restoration: group 3: 5.25% NaOCl - 17% EDTA and AH Plus Cleaner; group 4: 5.25% NaOCl - 17% EDTA and 100% ethanol; group 5: 5.25% NaOCl + 18% HEDP and AH Plus Cleaner; group 6: 5.25% NaOCl + 18% HEDP and 100% ethanol. µTBS testing was carried out for each subgroup and interfaces were analyzed through SEM. The µTBS was analyzed with Welch statistics and the post-hoc Games-Howell test for multiple comparisons (p < 0.05). RESULTS: Statistically significant differences were observed between test groups and control groups (p < 0.001), except for group 3 (p = 0.349). No significant differences were recorded between groups 1A (27.5 ± 8.7 MPa), 2A (32.6 ± 14.5 MPa) and 3 (27.3 ± 14.3 MPa). Group 6 (48.6 ± 11.6 MPa) exhibited the highest µTBS among all tested groups. For groups 1 and 2, significantly higher values were recorded for subgroups 1B (39.3 ± 7.6 MPa) and 2B (43.8 ± 8.3 MPa) compared to subgroups 1A (27.5 ± 8.7 MPa) and 2A (32.6 ± 14.5 MPa). CONCLUSION: When using NaOCl as root canal irrigant, it is advisable to delay the placement of a post-endodontic-treatment restoration.


Assuntos
Colagem Dentária , Cimentos Dentários , Cavidade Pulpar , Análise do Estresse Dentário , Dentina , Ácido Edético , Teste de Materiais , Irrigantes do Canal Radicular , Preparo de Canal Radicular , Hipoclorito de Sódio
3.
Sci Total Environ ; 772: 145029, 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-33770863

RESUMO

Enhanced electrokinetic remediation (EKR) allows the rapid remediation of heavy metal-contaminated clay, but the impacts of this process on soil micro-ecology have rarely been evaluated. In this study, nitric acid, acetic acid, and EDTA were applied for enhancement of EKR and the effects on Cd removal, soil enzyme activity, and soil bacterial communities (SBCs) were determined. Nitric acid and acetic acid allowed 93.2% and 91.8% Cd removal, respectively, and EDTA treatment resulted in 40.4% removal due to the formation of negatively charged EDTA-Cd complexes, resulting in opposing directions of Cd electromigration and electroosmosis flow and slow electromigration rate caused by low voltage drop. Activities of soil beta-glucosidase, acid phosphatase, and urease, were all reduced by enhanced EKR treatment, especially nitric acid treatment, by 46.2%, 58.8% and 57.7%, respectively. The SBCs were analyzed by high-throughput sequencing and revealed significantly increased diversity for acetic acid treatment, no effect for EDTA treatment, and reduced diversity for nitric acid treatment. Compared with nitric acid and EDTA, acetic acid treatment enhanced EKR for higher Cd removal and improved biodiversity.


Assuntos
Recuperação e Remediação Ambiental , Poluentes do Solo , Ácido Acético , Cádmio , Argila , Ácido Edético , Ácido Nítrico , Solo , Poluentes do Solo/análise
4.
PLoS One ; 16(3): e0247356, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33667247

RESUMO

BACKGROUND: Hydroxychloroquine (HCQ) and azithromycin (AZM) are antimalarial drugs recently reported to be active against severe acute respiratory syndrome coronavirus- 2 (SARS-CoV-2), which is causing the global COVID-19 pandemic. In an emergency response to the pandemic, we aimed to develop a quantitation method for HCQ, its metabolites desethylhydroxychloroquine (DHCQ) and bisdesethylchloroquine (BDCQ), and AZM in human plasma. METHODS: Liquid chromatography tandem mass spectrometry was used to develop the method. Samples (20 µL) are extracted by solid-phase extraction and injected onto the LC-MS/MS system equipped with a PFP column (2.0 × 50 mm, 3 µm). ESI+ and MRM are used for detection. Ion pairs m/z 336.1→247.1 for HCQ, 308.1→179.1 for DHCQ, 264.1→179.1 for BDCQ, and 749.6→591.6 for AZM are selected for quantification. The ion pairs m/z 342.1→253.1, 314.1→181.1, 270.1→181.1, and 754.6→596.6 are selected for the corresponding deuterated internal standards (IS) HCQ-d4, DHCQ-d4, BDCQ-d4, and AZM-d5. The less abundant IS ions from 37Cl were used to overcome the interference from the analytes. RESULTS: Under optimized conditions, retention times are 0.78 min for BDCQ, 0.79 min for DHCQ, 0.92 min for HCQ and 1.87 min for AZM. Total run time is 3.5 min per sample. The calibration ranges are 2-1000 ng/mL for HCQ and AZM, 1-500 ng/mL for DHCQ and 0.5-250 ng/mL for BDCQ; samples above the range are validated for up to 10-fold dilution. Recoveries of the method ranged from 88.9-94.4% for HCQ, 88.6-92.9% for DHCQ, 88.7-90.9% for BDCQ, and 98.6%-102% for AZM. The IS normalized matrix effect were within (100±10) % for all 4 analytes. Blood samples are stable for at least 6 hr at room temperature. Plasma samples are stable for at least 66 hr at room temperature, 38 days at -70°C, and 4 freeze-thaw cycles. CONCLUSIONS: An LC-MS/MS method for simultaneous quantitation of HCQ, DHCQ, BDCQ, and AZM in human plasma was developed and validated for clinical studies requiring fast turnaround time and small samples volume.


Assuntos
Antibacterianos/sangue , Antimaláricos/sangue , Azitromicina/sangue , Cloroquina/análogos & derivados , Hidroxicloroquina/análogos & derivados , Hidroxicloroquina/sangue , Coleta de Amostras Sanguíneas/métodos , Cloroquina/sangue , Cromatografia Líquida de Alta Pressão/métodos , Monitoramento de Medicamentos/métodos , Ácido Edético/sangue , Humanos , Limite de Detecção , Espectrometria de Massas em Tandem/métodos
5.
Cells ; 10(3)2021 02 27.
Artigo em Inglês | MEDLINE | ID: mdl-33673459

RESUMO

The article describes the rationale for the administration of zinc-chelating agents in COVID-19 patients. In a previous work I have highlighted that the binding of the SARS-CoV spike proteins to the zinc-metalloprotease ACE2 has been shown to induce ACE2 shedding by activating the zinc-metalloprotease ADAM17, which ultimately leads to systemic upregulation of ACE2 activity. Moreover, based on experimental models, it was also shown the detrimental effect of the excessive systemic activity of ACE2 through its downstream pathways, which leads to "clinical" manifestations resembling COVID-19. In this regard, strong upregulation of circulating ACE2 activity was recently reported in COVID-19 patients, thus supporting the previous hypothesis that COVID-19 may derive from upregulation of ACE2 activity. Based on this, a reasonable hypothesis of using inhibitors that curb the upregulation of both ACE2 and ADAM17 zinc-metalloprotease activities and consequent positive feedback-loops (initially triggered by SARS-CoV-2 and subsequently sustained independently on viral trigger) is proposed as therapy for COVID-19. In particular, zinc-chelating agents such as citrate and ethylenediaminetetraacetic acid (EDTA) alone or in combination are expected to act in protecting from COVID-19 at different levels thanks to their both anticoagulant properties and inhibitory activity on zinc-metalloproteases. Several arguments are presented in support of this hypothesis and based on the current knowledge of both beneficial/harmful effects and cost/effectiveness, the use of chelating agents in the prevention and therapy of COVID-19 is proposed. In this regard, clinical trials (currently absent) employing citrate/EDTA in COVID-19 are urgently needed in order to shed more light on the efficacy of zinc chelators against SARS-CoV-2 infection in vivo.


Assuntos
/tratamento farmacológico , Quelantes/farmacologia , Ácido Cítrico/farmacologia , Ácido Edético/farmacologia , Sistema Renina-Angiotensina/efeitos dos fármacos , Zinco/metabolismo , Proteína ADAM17/metabolismo , Anticoagulantes/farmacologia , /terapia , Descoberta de Drogas , Humanos , Imunização Passiva/efeitos adversos , Regulação para Cima/efeitos dos fármacos
6.
Int J Nanomedicine ; 16: 715-724, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33542626

RESUMO

Objective: The coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is now rapidly spreading globally. Serological tests are an important method to assist in the diagnosis of COVID-19, used for epidemiological investigations. In this study, we aimed to investigate the impact of different types of vacuum collection tubes on the detection of SARS-CoV-2 IgM and IgG antibodies, using the colloidal gold immunochromatographic assay (GICA). Patients and Methods: A total of 112 patients with COVID-19 and 200 healthy control subjects with no infection were enrolled in this study. Their serum and plasma were collected into four different types of vacuum blood collection tubes. SARS-CoV-2 IgM and IgG specific antibodies in the plasma and serum were then detected by GICA and chemiluminescence assay (CA), respectively. In addition, the particle sizes of different colloidal gold solutions in the presence of different anticoagulants and coagulants were evaluated by both laser diffraction (Malvern) and confocal laser microscope, respectively. Results: Our results revealed that anticoagulated plasma with EDTA-K2 improved the positive detection rate of SARS-CoV-2 IgM antibodies. Furthermore, our results shown that the detection results by GICA and CA were highly consistent, especially, the results of EDTA-K2 anticoagulated plasma detected by GICA was more consistent with CA results. We confirmed that EDTA-K2 could improve the detection sensitivity of SARS-CoV-2 IgG antibodies by chelating excessive colloidal gold compared with sodium citrate or lithium heparin, these methodologies did not appear to cause false positives. Colloidal gold particles could be chelated and aggregated by EDTA-K2, but not by sodium citrate, lithium heparin and coagulants. Conclusion: GICA is widely used to detect antibodies for the advantages of convenient, fast, low cost, suitable for screening large sample and require minimal equipment. In this study, we found that EDTA-K2 amplified the positive antibody signal by chelating colloidal gold and improved the detection sensitivity of SARS-CoV-2 IgM and IgG antibodies when using the GICA. Therefore, we suggested that EDTA-K2 anticoagulated plasma was more suitable for the detection of SARS-CoV-2 antibodies.


Assuntos
Anticorpos Antivirais/isolamento & purificação , Quelantes/química , Ácido Edético/química , Coloide de Ouro/química , Imunoensaio/métodos , Imunoglobulina G/isolamento & purificação , Imunoglobulina M/isolamento & purificação , /imunologia , Adulto , Anticorpos Antivirais/sangue , Especificidade de Anticorpos/imunologia , /imunologia , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Masculino , Pessoa de Meia-Idade , Peso Molecular , Tamanho da Partícula , Polímeros/química , Sensibilidade e Especificidade
7.
Br J Radiol ; 94(1120): 20201356, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33571001

RESUMO

OBJECTIVES: To investigate the impact of total variation regularized expectation maximization (TVREM) reconstruction on the image quality of 68Ga-PSMA-11 PET/CT using phantom and patient data. METHODS: Images of a phantom with small hot sphere inserts and 20 prostate cancer patients were acquired with a digital PET/CT using list-mode and reconstructed with ordered subset expectation maximization (OSEM) and TVREM with seven penalisation factors between 0.01 and 0.42 for 2 and 3 minutes-per-bed (m/b) acquisition. The contrast recovery (CR) and background variability (BV) of the phantom, image noise of the liver, and SUVmax of the lesions were measured. Qualitative image quality was scored by two radiologists using a 5-point scale (1-poor, 5-excellent). RESULTS: The performance of CR, BV, and image noise, and the gain of SUVmax was higher for TVREM 2 m/b groups with the penalization of 0.07 to 0.28 compared to OSEM 3 m/b group (all p < 0.05). The image noise of OSEM 3 m/b group was equivalent to TVREM 2 and 3 m/b groups with a penalization of 0.14 and 0.07, while lesions' SUVmax increased 15 and 20%. The highest qualitative score was attained at the penalization of 0.21 (3.30 ± 0.66) for TVREM 2 m/b groups and the penalization 0.14 (3.80 ± 0.41) for 3 m/b group that equal to or greater than OSEM 3 m/b group (2.90 ± 0.45, p = 0.2 and p < 0.001). CONCLUSIONS: TVREM improves lesion contrast and reduces image noise, which allows shorter acquisition with preserved image quality for PSMA PET/CT. ADVANCES IN KNOWLEDGE: TVREM reconstruction with optimized penalization factors can generate higher quality PSMA-PET images for prostate cancer diagnosis.


Assuntos
Ácido Edético/análogos & derivados , Interpretação de Imagem Assistida por Computador/métodos , Oligopeptídeos , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons/métodos , Neoplasias da Próstata/diagnóstico por imagem , Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Próstata/diagnóstico por imagem , Reprodutibilidade dos Testes
8.
J Zoo Wildl Med ; 51(4): 999-1006, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33480581

RESUMO

Anticoagulants are employed to prevent clotting and preserve cellular morphology for clinical pathology tests. Lithium heparin (LH) is the most frequently used anticoagulant in chelonians; however, dipotassium ethylenediaminetetraacetic acid (EDTA) may be superior in some species. Although eastern box turtles' (Terrapene carolina carolina) hematologic parameters are well studied, the effects of different anticoagulants on hematology in this species are unknown. This study evaluated the effects of LH and EDTA on hematologic values in free-living eastern box turtles (N = 59). Blood samples were collected from eastern box turtles in Illinois and immediately divided between LH and EDTA microtainers, and complete blood counts were performed on each sample. Grossly, plasma from EDTA blood samples was frequently and significantly hemolyzed. Blood mixed with LH had higher packed cell volume (PCV) (P = 0.04), white blood cell count (WBC) determined by Leukopet (P < 0.0001), WBC determined by blood film estimate (P < 0.0001), absolute heterophils (P = 0.007), absolute lymphocytes (P < 0.0001), and lower total solids (P < 0.0001) and absolute monocytes (P = 0.0001) than blood mixed with EDTA. All relative leukocyte counts were significantly different between the anticoagulants (P < 0.0001). EDTA apparently lysed turtle erythrocytes in this study, making it difficult to accurately count white blood cells and artificially lowering PCV. These findings demonstrate that EDTA should not be used in eastern box turtles.


Assuntos
Anticoagulantes/farmacologia , Ácido Edético/farmacologia , Heparina/farmacologia , Tartarugas/sangue , Animais , Coleta de Amostras Sanguíneas/veterinária , Hematócrito , Contagem de Leucócitos
9.
Methods Mol Biol ; 2261: 277-289, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33420996

RESUMO

Targeted protein quantification can be challenging in body fluids such as plasma with regard to sensitivity and selectivity. In this chapter, we present a protocol for the quantification of high mobility group box 1 protein (HMGB1) in plasma using an immunoaffinity liquid chromatography mass spectrometric assay (IA-LC-MSMS). The protocol provides detailed assay instructions involving sample proteolysis, peptide-targeted immunoprecipitation, and LC-MSMS-based read out.


Assuntos
Métodos Analíticos de Preparação de Amostras , Coleta de Amostras Sanguíneas , Cromatografia de Afinidade , Ácido Edético/farmacologia , Proteína HMGB1/sangue , Proteômica , Espectrometria de Massas em Tandem , Anticoagulantes/farmacologia , Humanos , Imunoprecipitação , Proteólise
10.
Br J Radiol ; 94(1119): 20201174, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33507812

RESUMO

OBJECTIVE: The optimal method for delineation of dominant intraprostatic lesions (DIL) for targeted radiotherapy dose escalation is unclear. This study evaluated interobserver and intermodality variability of delineations on biparametric MRI (bpMRI), consisting of T2 weighted (T2W) and diffusion-weighted (DWI) sequences, and 68Ga-PSMA-PET/CT; and compared manually delineated GTV contours with semi-automated segmentations based on quantitative thresholding of intraprostatic apparent diffusion coefficient (ADC) and standardised uptake values (SUV). METHODS: 16 patients who had bpMRI and PSMA-PET scanning performed prior to any treatment were eligible for inclusion. Four observers (two radiation oncologists, two radiologists) manually delineated the DIL on: (1) bpMRI (GTVMRI), (2) PSMA-PET (GTVPSMA) and (3) co-registered bpMRI/PSMA-PET (GTVFused) in separate sittings. Interobserver, intermodality and semi-automated comparisons were evaluated against consensus Simultaneous Truth and Performance Level Estimation (STAPLE) volumes, created from the relevant manual delineations of all observers with equal weighting. Comparisons included the Dice Similarity Coefficient (DSC), mean distance to agreement (MDA) and other metrics. RESULTS: Interobserver agreement was significantly higher (p < 0.05) for GTVPSMA (DSC: 0.822, MDA: 1.12 mm) and GTVFused (DSC: 0.787, MDA: 1.34 mm) than for GTVMRI (DSC: 0.705, MDA 2.44 mm). Intermodality agreement between GTVMRI and GTVPSMA was low (DSC: 0.440, MDA: 4.64 mm). Agreement between semi-automated volumes and consensus GTV was low for MRI (DSC: 0.370, MDA: 8.16 mm) and significantly higher for PSMA-PET (0.571, MDA: 4.45 mm, p < 0.05). CONCLUSION: 68Ga-PSMA-PET appears to improve interobserver consistency of DIL localisation vs bpMRI and may be more viable for simple quantitative delineation approaches; however, more sophisticated approaches to semi-automatic delineation factoring for patient- and disease-related heterogeneity are likely required. ADVANCES IN KNOWLEDGE: This is the first study to evaluate the interobserver variability of prostate GTV delineations with co-registered bpMRI and 68Ga-PSMA-PET.


Assuntos
Ácido Edético/análogos & derivados , Interpretação de Imagem Assistida por Computador/métodos , Imagem por Ressonância Magnética/métodos , Oligopeptídeos , Tomografia por Emissão de Pósitrons/métodos , Neoplasias da Próstata/diagnóstico por imagem , Compostos Radiofarmacêuticos , Idoso , Idoso de 80 Anos ou mais , Humanos , Masculino , Pessoa de Meia-Idade , Variações Dependentes do Observador , Próstata/diagnóstico por imagem , Reprodutibilidade dos Testes
11.
J Chromatogr A ; 1637: 461843, 2021 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-33412291

RESUMO

The manufacturability of therapeutic monoclonal antibodies is limited by the harsh conditions that antibodies are subjected to during the purification procedure, which in turn restricts the development of novel acid-sensitive antibodies. The gold standard for antibody purification, Protein A affinity chromatography, offers the selective capture of antibodies with great yields, but also poses a threat to the quality of the antibodies. Antibodies and Fc-fusion proteins risk forming aggregates as a consequence of the acidic elution from the Protein A ligands, compromising the potency and safety of the drug. Here, we present a novel, mild purification strategy based on a calcium-dependent ligand derived from Protein A, called ZCa. Antibodies captured on a high-capacity tetrameric ZCa resin in the presence of calcium can be eluted by removing the calcium ions through the addition of a chelator, and we describe the strive to find a sustainable alternative to the previously applied chelator EDTA. The naturally occurring chelator citrate is shown to seamlessly replace EDTA. Further buffer optimization reveals that the elution can be considerably improved by increasing the conductivity through the addition of 300 mM sodium chloride, leading to a very concentrated eluate. Remarkably, merely sodium chloride at a concentration of 50 mM is proven to be sufficient for calcium-dependent antibody release in a cost-efficient manner. Antibodies of subclasses IgG2 and IgG4 are eluted with sodium chloride at neutral pH and IgG1 at pH 6, due to varying affinities for the tetrameric ZCa, ranging between 90-780 nM. The mild elution of an IgG4 antibody eliminated the formation of aggregates, which constituted as much as 34% of all eluted antibody from MabSelect SuRe at pH 3. This novel purification strategy thus combines the valuable qualities of a Protein A resin, by providing high selectivity and a recovery of 88-99%, with an exceptionally mild elution step similar to ion-exchange chromatography, rendering considerably more functional antibody.


Assuntos
Anticorpos Monoclonais/isolamento & purificação , Resinas Sintéticas/química , Cloreto de Sódio/química , Proteína Estafilocócica A/química , Animais , Células CHO , Cálcio/química , Cromatografia por Troca Iônica , Citratos/química , Cricetinae , Cricetulus , Cristalografia por Raios X , Ácido Edético/química , Humanos , Concentração de Íons de Hidrogênio , Imunoglobulina G/química , Ligantes , Peso Molecular
12.
Ecotoxicol Environ Saf ; 208: 111607, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396127

RESUMO

The present study aimed to explore the effect of synthetic and naturally occurring chelators, EDTA and citric acid (CA), respectively, on changes in physiological and biochemical factors including cell death, level of mercury ions accumulation, malondialdehyde (MDA) content, total phenol and total flavonoids, anthocyanins and DPPH free radical scavenging activity, in the leaves of okra (Abelmoschus esculentus L.) plants exposed to mercury stress. In addition, polyphenolic compounds profile was assessed by high-performance liquid chromatography. The okras were planted in completely controlled hydroponic conditions (Hoagland solution). After they reached the four-leaf stage, they were treated simultaneously with different concentrations of HgCl2, EDTA and CA chelators, and their combination for one month. At the stage of maturity, the physiological and biochemical factors of the plant leaves were measured. The results showed that with the application of higher concentration of HgCl2, cell death, level of shoot and root Hg2+ content and root MDA, total phenols and total flavonoids, anthocyanin content, and DPPH free radical scavenging activity were increased. Also, the results indicated that okra plants have high biomass and a high rate of Hg mobilization and accumulation in the shoot versus the roots (TF=2.152 for the plants treated with 60 mg L-1 Hg2+), hence, can be considered as Hg hyperaccumulator plant for the phytoremediation of Hg-polluted soils and waters. In the Hg-treated plants changes in their phenolic profile were induced, and the increase of chlorogenic acid, rosmaric acid, apigenin, quercetin and rutin content was observed. The application of EDTA and CA improved the toxic effects of Hg2+, by modifying phenolic compounds, chelating Hg2+, and its proper compartmentation, while EDTA outperformed CA in this respect. Based on the results, it could be concluded that due to the high biomass and growth of okra in the presence of Hg2+, this plant is suitable for phytoremediation of soil and water contaminated with mercury. In addition, EDTA and CA can play a significant role in removing this toxic metal through transferring it from the culture medium to the plant.


Assuntos
Abelmoschus/efeitos dos fármacos , Ácido Cítrico/farmacologia , Ácido Edético/farmacologia , Mercúrio/toxicidade , Fenóis/metabolismo , Poluentes do Solo/toxicidade , Abelmoschus/crescimento & desenvolvimento , Abelmoschus/metabolismo , Fenômenos Bioquímicos/efeitos dos fármacos , Biodegradação Ambiental , Biomassa , Malondialdeído/metabolismo , Mercúrio/análise , Fenóis/análise , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Solo/química , Poluentes do Solo/análise
13.
Cochrane Database Syst Rev ; 1: CD011302, 2021 01 18.
Artigo em Inglês | MEDLINE | ID: mdl-33461239

RESUMO

BACKGROUND: Anaemia is a condition where the number of red blood cells (and consequently their oxygen-carrying capacity) is insufficient to meet the body's physiological needs. Fortification of wheat flour is deemed a useful strategy to reduce anaemia in populations. OBJECTIVES: To determine the benefits and harms of wheat flour fortification with iron alone or with other vitamins and minerals on anaemia, iron status and health-related outcomes in populations over two years of age. SEARCH METHODS: We searched CENTRAL, MEDLINE, Embase, CINAHL, 21 other databases and two trials registers up to 21 July 2020, together with contacting key organisations to identify additional studies. SELECTION CRITERIA: We included cluster- or individually-randomised controlled trials (RCTs) carried out among the general population from any country, aged two years and above. The interventions were fortification of wheat flour with iron alone or in combination with other micronutrients. We included trials comparing any type of food item prepared from flour fortified with iron of any variety of wheat DATA COLLECTION AND ANALYSIS: Two review authors independently screened the search results and assessed the eligibility of studies for inclusion, extracted data from included studies and assessed risks of bias. We followed Cochrane methods in this review. MAIN RESULTS: Our search identified 3538 records, after removing duplicates. We included 10 trials, involving 3319 participants, carried out in Bangladesh, Brazil, India, Kuwait, Philippines, South Africa and Sri Lanka. We identified two ongoing studies and one study is awaiting classification. The duration of interventions varied from 3 to 24 months. One study was carried out among adult women and one trial among both children and nonpregnant women. Most of the included trials were assessed as low or unclear risk of bias for key elements of selection, performance or reporting bias. Three trials used 41 mg to 60 mg iron/kg flour, three trials used less than 40 mg iron/kg and three trials used more than 60 mg iron/kg flour. One trial used various iron levels based on type of iron used: 80 mg/kg for electrolytic and reduced iron and 40 mg/kg for ferrous fumarate. All included studies contributed data for the meta-analyses. Iron-fortified wheat flour with or without other micronutrients added versus wheat flour (no added iron) with the same other micronutrients added Iron-fortified wheat flour with or without other micronutrients added versus wheat flour (no added iron) with the same other micronutrients added may reduce by 27% the risk of anaemia in populations (risk ratio (RR) 0.73, 95% confidence interval (CI) 0.55 to 0.97; 5 studies, 2315 participants; low-certainty evidence). It is uncertain whether iron-fortified wheat flour with or without other micronutrients reduces iron deficiency (RR 0.46, 95% CI 0.20 to 1.04; 3 studies, 748 participants; very low-certainty evidence) or increases haemoglobin concentrations (in g/L) (mean difference MD 2.75, 95% CI 0.71 to 4.80; 8 studies, 2831 participants; very low-certainty evidence). No trials reported data on adverse effects in children (including constipation, nausea, vomiting, heartburn or diarrhoea), except for risk of infection or inflammation at the individual level. The intervention probably makes little or no difference to the risk of Infection or inflammation at individual level as measured by C-reactive protein (CRP) (mean difference (MD) 0.04, 95% CI -0.02 to 0.11; 2 studies, 558 participants; moderate-certainty evidence). Iron-fortified wheat flour with other micronutrients added versus unfortified wheat flour (nil micronutrients added) It is unclear whether wheat flour fortified with iron, in combination with other micronutrients decreases anaemia (RR 0.77, 95% CI 0.41 to 1.46; 2 studies, 317 participants; very low-certainty evidence). The intervention probably reduces the risk of iron deficiency (RR 0.73, 95% CI 0.54 to 0.99; 3 studies, 382 participants; moderate-certainty evidence) and it is unclear whether it increases average haemoglobin concentrations (MD 2.53, 95% CI -0.39 to 5.45; 4 studies, 532 participants; very low-certainty evidence). No trials reported data on adverse effects in children. Nine out of 10 trials reported sources of funding, with most having multiple sources. Funding source does not appear to have distorted the results in any of the assessed trials. AUTHORS' CONCLUSIONS: Fortification of wheat flour with iron (in comparison to unfortified flour, or where both groups received the same other micronutrients) may reduce anaemia in the general population above two years of age, but its effects on other outcomes are uncertain. Iron-fortified wheat flour in combination with other micronutrients, in comparison with unfortified flour, probably reduces iron deficiency, but its effects on other outcomes are uncertain. None of the included trials reported data on adverse side effects except for risk of infection or inflammation at the individual level. The effects of this intervention on other health outcomes are unclear. Future studies at low risk of bias should aim to measure all important outcomes, and to further investigate which variants of fortification, including the role of other micronutrients as well as types of iron fortification, are more effective, and for whom.


Assuntos
Anemia/dietoterapia , Farinha , Alimentos Fortificados , Ferro/administração & dosagem , Triticum , Adolescente , Adulto , Anemia/sangue , Criança , Pré-Escolar , Ácido Edético/administração & dosagem , Feminino , Compostos Férricos/administração & dosagem , Compostos Ferrosos/administração & dosagem , Fumaratos , Hemoglobina A/análise , Humanos , Lactente , Ferro/deficiência , Masculino , Micronutrientes/administração & dosagem , Pessoa de Meia-Idade , Ensaios Clínicos Controlados Aleatórios como Assunto , Adulto Jovem
14.
Int J Hematol ; 113(1): 5-9, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33389659

RESUMO

In recent years, multipotent mesenchymal stromal cells (MSCs) have demonstrated tremendous potential for use in regenerative medicine. CXCR4, the receptor for CXCL12, is highly expressed by bone marrow (BM) MSCs and the CXCR4/CXCL12 axis has been shown to be important for migration and homing of BM-MSCs. Typically, MSCs used for clinical applications are collected after culture expansion using enzymatic methods, such as trypsin. Here, we compared different commercially available enzymatic and non-enzymatic methods for collection and dissociation of MSCs from culture plastics and their effects on CXCR4 expression by MSCs. We found that whereas non-enzymatic dissociation buffers and methods maintained CXCR4 expression, all tested enzymatic dissociation solutions dramatically decreased expression of CXCR4. We, therefore, strongly recommend the use of non-enzymatic dissociation methods, followed by filtration through a cell strainer to obtain single cell suspensions, in order to preserve maximal CXCR4 expression and optimal homing of cells.


Assuntos
Células da Medula Óssea/metabolismo , Separação Celular/métodos , Expressão Gênica , Células-Tronco Mesenquimais/metabolismo , Receptores CXCR4/genética , Receptores CXCR4/metabolismo , Tripsina , Movimento Celular , Células Cultivadas , Quimiocina CXCL12 , Ácido Edético , Humanos
15.
Chemosphere ; 271: 129513, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33429262

RESUMO

Phytoremediation technology is an eco-friendly technology for the treatment of the polluted environment. Conversely, the natural and synthetic amendments have been revealed to improve the heavy metal phytoextraction from polluted soils with hyperaccumulation and/or non-hyper accumulating plants. This study evaluated the synergistic effect of biochar (BC) and EDTA to enhance phytoextraction of heavy metal lead (Pb) from artificially polluted soil by Brassica juncea. The BC and EDTA amendment enhanced the growth and survival of B. juncea under Pb stress environment. BC and EDTA significantly increased the biomass of B. juncea and significantly increased the total chlorophyll content in the combined amendment of BC and EDTA (22.2 mg/g) compared to the individual amendment of BC (12.8 mg/g) and EDTA (12.2 mg/g) respectively. The combined use of EDTA and biochar showed enhanced Pb uptake (60.2 mg/g) compared to control (10.0 mg/g). The order of Pb uptake was found to be BC + EDTA (60.2 mg/g) ˃ EDTA (23.5 mg/g) ˃ BC (22. 0 mg/g) ˃ control (10.0 mg/g). The maximum activity of SOD (35.2 ± 1.2 U/mg), POD (47.0 ± 1.8 U/mg) and CAT (28.0 ± 1.0 U/mg) was obtained in the mixed application of EDTA and BC. The obtained results revealed that the combined use of BC and EDTA was the most advantageous option for the treatment of Pb contaminated soil as compared to individual amendments.


Assuntos
Mostardeira , Poluentes do Solo , Biodegradação Ambiental , Carvão Vegetal , Ácido Edético , Chumbo , Solo , Poluentes do Solo/análise
16.
Ecotoxicol Environ Saf ; 209: 111858, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33385680

RESUMO

A novel amino-functionalized magnetic adsorbent (Fe3O4@C@DETA) was developed for adsorption of Cr(III) and Cr(III)-EDTA from wastewater. Fe3O4@C@DETA were successfully prepared by modification of Fe3O4@C with diethylenediamine (DETA), which exhibits a core-shell structure and sufficient saturation magnetization. Fe3O4@C@DETA exhibits much better adsorption performance for Cr(III) and its chelates than the Fe3O4@C because of newly introduced amino active sites. The enhanced adsorption capacity of Fe3O4@C@DETA for Cr(III) is 44.74 mg g-1 (at 25 °C and pH 3.0), which is due to the surface coordination with the newly introduced amino functional sites. The Cr(III)-EDTA anions as a whole was adsorbed through the electrostatic interaction with protonated amino species of the Fe3O4@C@DETA and have maximum adsorption capacity of 47.27 mg g-1 (at 25 °C and pH 3.0). The adsorption data of free and EDTA coordinated Cr(III) were followed the Langmuir equation, while the adsorption dynamics was well explained by pseudo second order model indicating the chemical nature of adsorption process. The higher concentration of Ca2+ ions in the wastewater compete for adsorption sites and inhibit the Cr(III) removal, while on other hand Ca2+ ions promotes the adsorption of Cr(III)-EDTA, because of electrostatic interaction with adsorbent active sits. Furthermore, the adsorbent can be easily separated by external magnetic field and regenerated in acidic solution. The adsorbent is stable, recyclable and have more than 75% regeneration efficiency and can be repeatedly used in the adsorption process.


Assuntos
Cromo/análise , Poluentes Químicos da Água/análise , Purificação da Água/métodos , Adsorção , Carbono , Ácido Edético , Concentração de Íons de Hidrogênio , Fenômenos Magnéticos , Magnetismo , Águas Salinas , Salinidade , Águas Residuárias/química , Água/química
17.
Methods Mol Biol ; 2167: 147-169, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-32712919

RESUMO

Kink-turns are important RNA structural modules that facilitate long-range tertiary interactions and form binding sites for members of the L7Ae family of proteins. Present in a wide variety of functional RNAs, kink-turns play key organizational roles in many RNA-based cellular processes, including translation, modification, and tRNA biogenesis. It is important to determine the contribution of kink-turns to the overall architecture of resident RNAs, as these modules dictate ribonucleoprotein (RNP) assembly and function. This chapter describes a site-directed, hydroxyl radical-mediated footprinting strategy that utilizes L7Ae-tethered chemical nucleases to experimentally validate computationally identified kink-turns in any RNA and under a wide variety of conditions. The work plan described here uses the catalytic RNase P RNA as an example to provide a blueprint for using this footprinting method to map RNA-protein interactions in other RNP complexes.


Assuntos
Proteínas Arqueais/química , Pegada de DNA/métodos , Ácido Edético/análogos & derivados , Radical Hidroxila/química , Dobramento de RNA/genética , RNA/química , Ribonuclease P/metabolismo , Sítios de Ligação , Ácido Edético/química , Conformação de Ácido Nucleico , Motivos de Nucleotídeos/genética , Ligação Proteica , RNA Catalítico/genética , RNA Catalítico/metabolismo , Transcrição Reversa , Ribonuclease P/genética , Ribonucleoproteínas/química , Ribonucleoproteínas/metabolismo , Análise de Sequência de DNA
18.
Clin Nucl Med ; 46(1): 69-70, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33208621

RESUMO

A 74-year-old man with a history of prostate cancer with proven osseous metastatic disease underwent Ga-prostate-specific membrane antigen (PSMA) PET/CT under antiandrogen therapy. The scan revealed a long segment of increased PSMA tracer uptake within the right sciatic nerve, which appeared edematous and swollen, and the respective ganglia. Clinically, the patient suffered from pain and paresis in the right leg. As infiltration of a long segment of a single nerve seems unlikely, primarily neuronal disease such as neuritis (induced by metastases or radiotherapy) was considered. The observed uptake of PSMA-targeting PET tracers may then represent a peripheral nerve disorder.


Assuntos
Ácido Edético/análogos & derivados , Gânglios/metabolismo , Nervos Periféricos/metabolismo , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Idoso , Transporte Biológico , Neoplasias Ósseas/secundário , Ácido Edético/metabolismo , Gânglios/diagnóstico por imagem , Gânglios/patologia , Humanos , Masculino , Nervos Periféricos/diagnóstico por imagem , Nervos Periféricos/patologia , Neoplasias da Próstata/diagnóstico por imagem , Neoplasias da Próstata/tratamento farmacológico , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia
19.
Clin Nucl Med ; 46(3): 264-265, 2021 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-33351509

RESUMO

ABSTRACT: A 61-year-old woman was diagnosed with progressive metastatic radioactive iodine refractory differentiated thyroid cancer and underwent multitargeted tyrosine kinase inhibitor (TKI) Donafenib therapy. After 12 months of therapy, she developed resistance to TKI. Considering the limited treatment options available, a 68Ga-prostate-specific membrane antigen (PSMA)-617 PET/CT was conducted for potential therapeutic target. 68Ga-PSMA-617 PET/CT showed an intense radiotracer uptake in inferior vena cava, which was subsequently confirmed as tumor thrombus. Thus, this patient is a rare case in which the tumor thrombus from thyroid cancer was discovered in inferior vena cava via 68Ga-PSMA-617 PET/CT.


Assuntos
Ácido Edético/análogos & derivados , Oligopeptídeos , Tomografia Computadorizada com Tomografia por Emissão de Pósitrons , Trombose/complicações , Trombose/diagnóstico por imagem , Veia Cava Inferior/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias da Glândula Tireoide/complicações , Veia Cava Inferior/patologia , Veia Cava Inferior/fisiopatologia
20.
Chemosphere ; 263: 127802, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33297000

RESUMO

Effective removal of cadmium (Cd) from Chlamys farreri by introducing ZnSO4, EDTA-Na2, and sodium citrate into seawater has previously been reported. However, some mechanisms underlying this removal are not clear. To address this lack of clarity, the present study aimed to investigate the changes of Cd forms in Chlamys farreri from treatment of these additives and analyze the physiological and biochemical responses by comparing the changes over treatment time in Catalase (CAT), Superoxide dismutase (SOD), and Glutathione s-transferase (GST) activity, as well as Malonaldehyde (MDA) concentration and glycogen level. Three forms of Cd, including protein -Cd, liberated Cd, and amino acid/peptide -Cd, were found, and they were sorted according to their Cd content into the following groups: protein -Cd > liberated Cd > amino acid/peptide-Cd. The removal rates of the three forms of Cd were 43.2%, 59.5%, and 59.0%, respectively, using ZnSO4 and EDTA-Na2. Additionally, a significant increase in Zn content was observed, which may suggest that reduction of bound Cd was partly due to the displacement of Cd by Zn. Moreover, Cd depuration using the additives can mitigate oxidative stress only in the first 12 h. Glycogen content continued to reduce over time, inferring that the healthy status of Chlamys farreri under treatment of the additives containing Zn can only be maintained within 12 h for excreting Cd when linking these physiological responses with the ability of the additives to remove Cd only in a short time, i.e. 12 h. The results indicated that Cd should be removed from Chlamys farreri for practical reasons.


Assuntos
Produtos Biológicos , Pectinidae , Animais , Cádmio , Ácido Edético , Estresse Oxidativo , Citrato de Sódio , Superóxido Dismutase/metabolismo
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