Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 45
Filtrar
2.
Free Radic Res ; 54(1): 43-56, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31909639

RESUMO

Caspase-3 is involved in apoptosis. Here, we examine whether hypochlorous acid (HOCl), a final product of myeloperoxidase (MPO), is a modulator of caspase-3 at relatively low concentrations and also its application on metaphase II mouse oocytes. We utilised caspase-3 activity assay, TUNEL assay, the CellEvent caspase 3/7 fluorescent assay, and the MPO/hydrogen peroxide (H2O2) system on mouse oocytes with and without cumulus cells to examine whether low concentrations of HOCl mediate apoptosis by inhibition of caspase-3. A UV-visible spectrophotometer was used to study caspase-3 activity. To determine whether HOCl mediates apoptosis in mouse oocytes, two different concentrations (10 and 100 µM) of HOCl generated by the MPO/H2O2 system were used as treatments (10 µM had little effect on oocyte quality, while 100 µM showed significant deterioration). Induction of apoptotic cell death was determined by TUNEL Assay and the CellEvent caspase 3/7. HOCl mediates caspase-3 inactivation in a dose dependent manner. Subsequent addition of dithiothreitol caused recovery of caspase-3 activity indicating involvement of the oxidation of the Cys-thiol group. Accumulation of HOCl generated by MPO in the presence of caspase-3 also inhibits MPO but requires higher HOCl concentrations, indicating specificity of lower HOCl concentrations to inhibition of caspase-3. Exposure of oocytes to lower HOCl concentrations generated by MPO-H2O2 system prevents MPO-mediated apoptosis whereas exposure to higher HOCl (100 µM) showed apoptosis. Similar results were observed by using the CellEvent caspase 3/7 assay. Low concentrations of HOCl inhibit caspase-3 activity, and may play a role in regulating apoptosis, thus affecting oocyte quality.HighlightsCaspase-3 is involved in apoptosis pathway and loss of this regulation is seen in several diseases.These conditions are associated with inflammation and higher myeloperoxidase (MPO) activity.We examined whether hypochlorous acid (HOCl), generated by MPO, is a modulator of caspase-3.Caspase-3 activity showed a dose dependent decrease with HOCl and this reaction was reversible.HOCl modulates caspase-3 activity and may play a physiological role in regulating apoptosis.


Assuntos
Apoptose/efeitos dos fármacos , Caspase 3/efeitos dos fármacos , Ácido Hipocloroso/uso terapêutico , Animais , Feminino , Humanos , Masculino , Camundongos
3.
Molecules ; 24(24)2019 Dec 04.
Artigo em Inglês | MEDLINE | ID: mdl-31817098

RESUMO

Hailey-Hailey disease (HHD) is a rare, chronic and recurrent blistering disorder, characterized by erosions occurring primarily in intertriginous regions and histologically by suprabasal acantholysis. Mutation of the Golgi Ca2+-ATPase ATP2C1 has been identified as having a causative role in Hailey-Hailey disease. HHD-derived keratinocytes have increased oxidative-stress that is associated with impaired proliferation and differentiation. Additionally, HHD is characterized by skin lesions that do not heal and by recurrent skin infections, indicating that HHD keratinocytes might not respond well to challenges such as wounding or infection. Hypochlorous acid has been demonstrated in vitro and in vivo to possess properties that rescue both oxidative stress and altered wound repair process. Thus, we investigated the potential effects of a stabilized form of hypochlorous acid (APR-TD012) in an in vitro model of HHD. We found that treatment of ATP2C1-defective keratinocytes with APR-TD012 contributed to upregulation of Nrf2 (nuclear factor (erythroid-derived 2)-like 2). Additionally, APR TD012-treatment restored the defective proliferative capability of siATP2C1-treated keratinocytes. We also found that the APR-TD012 treatment might support wound healing process, due to its ability to modulate the expression of wound healing associated cytokines. These observations suggested that the APR-TD012 might be a potential therapeutic agent for HHD-lesions.


Assuntos
Ácidos/química , Ácido Hipocloroso/uso terapêutico , Soluções Hipotônicas/uso terapêutico , Pênfigo Familiar Benigno/tratamento farmacológico , Antioxidantes/metabolismo , ATPases Transportadoras de Cálcio/metabolismo , Linhagem Celular , Citocinas/genética , Citocinas/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Ácido Hipocloroso/farmacologia , Soluções Hipotônicas/farmacologia , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Queratinócitos/patologia , Fator 2 Relacionado a NF-E2/metabolismo , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Pênfigo Familiar Benigno/genética , Pênfigo Familiar Benigno/patologia , Espécies Reativas de Oxigênio/metabolismo , Soluções , Cicatrização/efeitos dos fármacos
4.
Medicina (Kaunas) ; 55(5)2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31126140

RESUMO

Background and objective: One of the reasons for thrombosis in chronic heart failure (CHF) might be reactive forms of oxygen activating platelets. The aim of this study was to evaluate the effect of oxidant hypochlorous acid (HOCl) on platelet aggregation and dityrosine concentration in CHF patients and healthy controls. Materials and Methods: CHF patients (n 67) and healthy (n 31) were investigated. Heart echoscopy, 6-min walking test, complete blood count, platelet aggregation, and dityrosine concentration were performed. Platelet aggregation and dityrosine concentration were measured in plasma samples after incubation with different HOCl concentrations (0.15, 0.0778, and 0.0389 mmol/L). Results: Platelet aggregation without oxidant was lower (p = 0.049) in CHF patients than in controls. The spontaneous platelet aggregation with oxidant added was higher in CHF patients (p = 0.004). Dityrosine concentration was also higher (p = 0.032) in CHF patients. Platelet aggregation was the highest in samples with the highest oxidant concentration in both healthy controls (p = 0.0006) and in CHF patients (p = 0.036). Platelet aggregation was higher in NYHA III group in comparison to NYHA II group (p = 0.0014). Concentration of dityrosine was significantly higher in CHF samples (p = 0.032). The highest concentration of dityrosine was obtained in NYHA IV group samples (p 0.05). Intensity of platelet aggregation, analyzed with ADP, was correlated with LV EF (r 0.42, p = 0.007). Dityrosine concentration was correlated with NYHA functional class (r 0.27, p 0.05). Conclusions: The increase in platelet aggregation in CHF and healthy controls shows the oxidant effect on platelets. The increase in dityrosine concentration in higher NYHA functional classes shows a higher oxidative stress in patients with worse condition.


Assuntos
Insuficiência Cardíaca/sangue , Ácido Hipocloroso/uso terapêutico , Agregação Plaquetária/fisiologia , Tirosina/análogos & derivados , Idoso , Feminino , Voluntários Saudáveis , Insuficiência Cardíaca/enzimologia , Insuficiência Cardíaca/fisiopatologia , Humanos , Ácido Hipocloroso/farmacocinética , Masculino , Pessoa de Meia-Idade , Estatísticas não Paramétricas , Tirosina/análise , Tirosina/sangue
5.
Med Oral Patol Oral Cir Bucal ; 23(6): e716-e722, 2018 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-30341268

RESUMO

BACKGROUND: The main objective of the present study is to evaluate the effects and possible benefits with regard to the postoperative period of lower third molar extractions, comparing the intraalveolar application of a bioadhesive gel of 0.2% chlorhexidine (CHX) to the use of a mouthwash with a super-oxidized solution, (SOS) Dermacyn® Wound Care (Oculus Innovative Sciences lnc., California, USA). MATERIAL AND METHODS: A randomized double-blind study was carried out in 20 patients with a split-mouth design, with a total of 40 extractions of symmetrically impacted bilateral lower third molars. Patients were divided into two groups, a control group (C = 20) and an experimental group (D = 20). Any infectious complications, wound healing, plaque accumulation in the stitches, and presence of trismus and inflammation were evaluated using the distance between different facial points, at three, eight, and fifteen days after extraction. Pain, swelling, and amount of analgesics taken were evaluated using the VAS scale throughout the 15 days following extraction. Tolerance to treatment was evaluated using a verbal scale. Results were statistically compared using the Student's t- and chi-squared tests. RESULTS: No statistically significant differences were found between the two groups with regard to infectious complications, swelling, or wound healing. Use of analgesics and self-reported pain levels were slightly lower in the experimental group than in the control group during days 6 and 7 of the study (p < 0.05). The global treatment tolerance was satisfactory and similar in both groups. CONCLUSIONS: Both CHX and SOS are effective at improving the postoperative period after extraction of lower third molars.


Assuntos
Clorexidina/administração & dosagem , Edema/prevenção & controle , Ácido Hipocloroso/uso terapêutico , Dente Serotino/cirurgia , Antissépticos Bucais/uso terapêutico , Dor Pós-Operatória/prevenção & controle , Complicações Pós-Operatórias/prevenção & controle , Hipoclorito de Sódio/uso terapêutico , Extração Dentária , Cicatrização/efeitos dos fármacos , Adulto , Método Duplo-Cego , Combinação de Medicamentos , Feminino , Géis , Humanos , Masculino , Mandíbula , Estudos Prospectivos
6.
BMC Pharmacol Toxicol ; 19(1): 37, 2018 07 03.
Artigo em Inglês | MEDLINE | ID: mdl-29970189

RESUMO

BACKGROUND: Topical administration of PR022, 0.05% hypochlorous acid (HOCl) in gel has been demonstrated to be beneficial in a chronic murine atopic dermatitis model. In a follow up study we tested a higher concentration (0.1%) of PR022 HOCl gel in comparison to the Janus kinase inhibitor tofacitinib, both of which are currently in clinical phase studies for treatment of human atopic dermatitis. METHODS: The effect of topically administered HOCl (0.1%) in gel was compared to a topical formulation of tofacitinib (0.5%) in a therapeutic setting on atopic dermatitis-like lesions in NC/Nga mice as well as itch behaviour. NC/Nga mice were sensitized with house dust mite allergen. After reaching visible lesions, mice were treated either topically with HOCl or tofacitinib or gel vehicle for 17 days. After termination of the study, dorsal root ganglia were isolated for ex vivo stimulation and skin samples were taken for cytokine determination in inflamed skin. RESULTS: When administered onto lesional skin of NC/Nga mice, both HOCl and tofacitinib reduced lesions and scratching behaviour. The reduced inflammatory response by HOCl and tofacitinib treatment was demonstrated by diminished inflammatory cytokines in affected skin tissue from NC/Nga mice. Dorsal root ganglia neurons re-stimulated with a range of mediators of itch showed a reduced response compared to the vehicle control mice, when isolated from tofacitinib or HOCl treated mice. CONCLUSIONS: These data indicate a similar beneficial potential of topical high dose PR022 HOCl (0.1%) in gel and tofacitinib, in a translational murine model of atopic dermatitis.


Assuntos
Dermatite Atópica/tratamento farmacológico , Ácido Hipocloroso/uso terapêutico , Piperidinas/uso terapêutico , Inibidores de Proteínas Quinases/uso terapêutico , Pirimidinas/uso terapêutico , Pirróis/uso terapêutico , Administração Tópica , Animais , Células Cultivadas , Citocinas/imunologia , Dermatite Atópica/imunologia , Modelos Animais de Doenças , Feminino , Gânglios Espinais/citologia , Imunoglobulina E/sangue , Camundongos , Neurônios/efeitos dos fármacos , Neurônios/imunologia , Pele/efeitos dos fármacos , Pele/imunologia
7.
Photodiagnosis Photodyn Ther ; 23: 347-352, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30053480

RESUMO

INTRODUCTION: The aim of this study is to evaluate the antimicrobial activity of hypochlorite solutions and reciprocating instrumentation associated with photodynamic therapy (PDT). METHODS: One hundred and thirty two root canals were enlarged up to #35 K-file and inoculated with E.faecalis for 14 days. The 132 samples were randomly divided into eleven groups (n = 12) and subjected to the following protocols: G1-distilled water + Reciproc R40 (control), G2-1% sodium hypochlorite (NaOCl) + Reciproc R40, G3-2.5% NaOCl + Reciproc R40; G4-1% calcium hypochlorite (Ca[OCl]2) + Reciproc R40, G5-2.5% Ca(OCl)2 + Reciproc R40; G6-PDT; G7-distilled water + Reciproc R40 + PDT, G8-1% NaOCl + Reciproc R40 + PDT, G9-2.5% NaOCl + Reciproc R40 + PDT; G10-1% Ca(OCl)2 + Reciproc R40 + PDT, G11-2.5% Ca(OCl)2 + Reciproc R40 + PDT. The percentage bacterial reduction was checked by counting the colony-forming units (CFUs) in 10 samples of each group. The remaining 2 samples of each group were submitted to scanning electronic microscopy (SEM) to illustrate the effectiveness of the proposed treatments. Data were subjected to one-way ANOVA followed by Tukey's post hoc test (α = 0.05). RESULTS: The greatest ability to promote bacterial reduction was observed in groups 8 (1% NaOCl + R40 + PDT), 9 (2.5% NaOCl + R40 + PDT), 10 (1% Ca[OCl]2 + R40 + PDT), and 11 (2.5% Ca[OCl]2 + R40 + PDT), with no significant difference between them (p < 0.05). CONCLUSIONS: The association of PDT with hypochlorite solutions and reciprocating instrumentation provides effective elimination of E.faecalis.


Assuntos
Enterococcus faecalis/efeitos dos fármacos , Ácido Hipocloroso/uso terapêutico , Azul de Metileno/uso terapêutico , Fotoquimioterapia/métodos , Fármacos Fotossensibilizantes/uso terapêutico , Preparo de Canal Radicular/métodos , Carga Bacteriana , Compostos de Cálcio/uso terapêutico , Terapia Combinada , Cavidade Pulpar/microbiologia , Relação Dose-Resposta a Droga , Humanos , Hipoclorito de Sódio/uso terapêutico
9.
Ginekol Pol ; 88(4): 222-223, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28509325

RESUMO

An 89-year-old woman was reffered to our Clinic with vulvar cancer. She also suffered from obesity [with body mass index (BMI) 35 kg/m2], persistent hypertension, diabetes mellitus type 2 treated with oral medications. In 2015 she underwent a surgery due to endometrial carcinoma. Total abdominal hysterectomy with bilateral salphingoophorectomy, omentectomy and pelvical node dissection was performed (histopathology revealed adenocarcinoma serosum G2; FIGO stage Ib). In January 2016 after vulvar ulceration biopsy plano-epithelial squamous vulvar cancer was diagnosed. She was referred to surgery. She has undergone an operation in October 2016. She was admitted to gynaecological unit at our institution. Physical examination revealed mutilated vulva with excised labia major, labia minor, and narrowing of vaginal orifice. The right side shown tumor 2.5 cm in diameter, with slough area and no deep infiltration and satellital nodule on the left labia majora 1cm in diameter. The cervix, vaginal wall, rectum and anus appeared normal. There were no enlarged lymph nodes at the inguinal area.


Assuntos
Carcinoma de Células Escamosas/cirurgia , Infecções por Enterobacteriaceae/terapia , Ácido Hipocloroso/uso terapêutico , Oxidantes/uso terapêutico , Infecções por Pseudomonas/terapia , Compostos de Prata/uso terapêutico , Infecção da Ferida Cirúrgica/terapia , Titânio/uso terapêutico , Neoplasias Vulvares/cirurgia , Administração Tópica , Idoso de 80 Anos ou mais , Farmacorresistência Bacteriana Múltipla , Feminino , Humanos , Morganella morganii , Pseudomonas aeruginosa , Retalhos Cirúrgicos , Irrigação Terapêutica/métodos , Vulvectomia/métodos , Cicatrização
10.
J Cosmet Dermatol ; 16(2): 162-167, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28370943

RESUMO

BACKGROUND: A pre-and postprocedure regime aimed at prevention of infection, reduction of inflammation and risk of scarring, is to enable optimal outcomes. OBJECTIVES: The role of a hypochlorous acid containing spray and translucent scar gel formulation that combines modified silicon oil with hypochlorous acid, was explored for pre- and postprocedure treatment and scar management. METHODS: For this purpose a literature review was conducted to explore the value of the technology used in pre-and postprocedural regimes. A panel of dermatologists and plastic surgeons who practice in the United States discussed the summarized search results, taking into account their current clinical practice. A nominal group process for consensus was used, followed by online reviews of the manuscript. RESULTS: Based on panel discussions, consensus was reached regarding clinical recommendations given for postprocedure treatment and scar management. The hypochlorous acid products are produced with electrolysis and are classified among biocidal substances. The technology has demonstrated efficacy and safety for pre-and postprocedure use. The safety of hypochlorous solution use demonstrated to be comparable to that of standard local antiseptics. Small studies demonstrated better results with the scar gel compared to silicone gel regarding the appearance of hypertrophic and keloid scars, relief of associated pruritus and pain. CONCLUSIONS: A postprocedure regime using this technology, aimed at preventing infection, reducing inflammation, and promoting healing is proposed to have benefits over current regimes as it appears to be effective, safe, and well tolerated.


Assuntos
Cicatriz/prevenção & controle , Ácido Hipocloroso/uso terapêutico , Cuidados Pós-Operatórios , Complicações Pós-Operatórias/prevenção & controle , Aerossóis , Procedimentos Cirúrgicos Dermatológicos , Géis , Humanos , Procedimentos Cirúrgicos Reconstrutivos
11.
J Hosp Infect ; 96(1): 42-48, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28420487

RESUMO

The combination of personal protective equipment (PPE) together with donning and doffing protocols was designed to protect British and Canadian military medical personnel in the Kerry Town Ebola Treatment Unit (ETU) in Sierra Leone. The PPE solution was selected to protect medical staff from infectious risks, notably Ebola virus, and chemical (hypochlorite) exposure. PPE maximized dexterity, enabled personnel to work in hot temperatures for periods of up to 2h, protected mucosal membranes when doffing outer layers, and minimized potential contamination of the doffing area with infectious material by reducing the requirement to spray PPE with hypochlorite. The ETU was equipped to allow medical personnel to provide a higher level of care than witnessed in many existing ETUs. This assured personnel working as part of the international response that they would receive as close to Western treatment standards as possible if they were to contract Ebola virus disease (EVD). PPE also enabled clinical interventions that are not seen routinely in West African EVD treatment regimens, whilst providing a robust protective barrier. Competency in using PPE was developed during a nine-day pre-deployment training programme. This allowed over 60 clinical personnel per deployment to practice skills in PPE in a simulated ETU and in classrooms. Overall, the training provided: (i) an evidence base underpinning the PPE solution chosen; (ii) skills in donning and doffing of PPE; (iii) personnel confidence in the selected PPE; and (iv) quantifiable testing of each individual's capability to don PPE, perform tasks and doff PPE safely.


Assuntos
Ebolavirus/patogenicidade , Pessoal de Saúde/educação , Doença pelo Vírus Ebola/transmissão , Transmissão de Doença Infecciosa do Paciente para o Profissional/prevenção & controle , Militares/educação , Equipamento de Proteção Individual/normas , Canadá , Doença pelo Vírus Ebola/prevenção & controle , Doença pelo Vírus Ebola/terapia , Humanos , Ácido Hipocloroso/efeitos adversos , Ácido Hipocloroso/uso terapêutico , Exposição Ocupacional/prevenção & controle , Oxidantes/efeitos adversos , Oxidantes/uso terapêutico , Equipamento de Proteção Individual/estatística & dados numéricos , Serra Leoa/epidemiologia , Reino Unido
12.
J Drugs Dermatol ; 16(3): 209-212, 2017 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-28301615

RESUMO

Surgical procedures are an important piece of a dermatologist's daily practice. Therefore, the optimization of post-surgical wound healing is an area of utmost importance and interest. Although low risk, one notable barrier to proper wound healing is surgical site infection.

In an attempt to mitigate this risk and improve surgical outcomes, multiple topical products continue to be used both pre- and postprocedure. Traditionally, this includes both topical antibiotics and antiseptics. However, these products are not without consequence.

The overuse of topical antibiotics as prophylaxis for infection has contributed to increased bacterial resistance, and in fact is no longer recommended by the American Academy of Dermatology in clean post surgical wounds. Topical antiseptics, including chlorhexidine and povidone-iodine, can have a cytotoxic effect on keratinocytes and may actually impede wound healing as a result. In addition, chlorhexidine in particular can produce both otologic and ocular toxic effects when used on the face. Emerging products, such as hypochlorous acid, may be a potential alternative to the more commonly used agents, as it has effective antimicrobial actions and minimal adverse effects. Therefore, the purpose of this review is to highlight several topical products used to optimize post-surgical wound healing and discuss both their efficacy and safety.

J Drugs Dermatol. 2017;16(3):209-212.

.


Assuntos
Antibacterianos/efeitos adversos , Anti-Infecciosos Locais/uso terapêutico , Antibioticoprofilaxia/efeitos adversos , Dermatite de Contato/etiologia , Infecção da Ferida Cirúrgica/prevenção & controle , Cicatrização/efeitos dos fármacos , Administração Tópica , Antibacterianos/administração & dosagem , Antibacterianos/uso terapêutico , Anti-Infecciosos Locais/administração & dosagem , Anti-Infecciosos Locais/efeitos adversos , Clorexidina/administração & dosagem , Clorexidina/efeitos adversos , Clorexidina/uso terapêutico , Farmacorresistência Bacteriana , Humanos , Ácido Hipocloroso/administração & dosagem , Ácido Hipocloroso/efeitos adversos , Ácido Hipocloroso/uso terapêutico , Queratinócitos/efeitos dos fármacos , Povidona-Iodo/administração & dosagem , Povidona-Iodo/efeitos adversos , Povidona-Iodo/uso terapêutico , Prurido/tratamento farmacológico , Resultado do Tratamento
13.
Minerva Chir ; 72(2): 121-124, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27841850

RESUMO

BACKGROUND: To evaluate antimicrobial properties of Dermacyn in abdominal infections, and to develop a new method for peritoneal lavage without using antibiotics. METHODS: One hundred and ten patients suffering from intestinal perforation (abdominal closed injury), who had been treated with opened surgical repair or partial intestinal resection, were enrolled in this study. In the study group, Dermacyn was used for peritoneal lavage. During the same period, 102 patients with intestinal perforation of abdominal closed injury and opened repair or partial intestinal resection were enrolled as a control group. For the control group, metronidazole physiological saline was used for peritoneal lavage. Patients from both groups had a postoperative indwelling peritoneal drainage tube. Five objective indicators were studied and compared at 24, 48, and 72 hours: volume of drainage, drainage fluid culture, blood routine test, C-reactive protein, calcitonin and antibiotics dose. RESULTS: There were no significant differences between the drainage volumes in two groups three days after the operation. There were significant differences in the nature of the drainage fluid, bacterial culture results, blood routine, C-reactive protein and procalcitonin between the two groups. In the experimental group, after the Dermacyn flushing, the average volume of peritoneal drainage fluid was reduced (from 30 to 5 mL) from 24 to 72 hours postoperatively. CONCLUSIONS: The use of Dermacyn as an antibiotic agent for intraoperative peritoneal lavage was effective in reducing the risk of infection, due to its broad-spectrum bactericidal effect. Dermacyn could be used safely to perform peritoneal flushing to clean the operation site.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Ácido Hipocloroso/uso terapêutico , Perfuração Intestinal/cirurgia , Lavagem Peritoneal , Peritonite/prevenção & controle , Hipoclorito de Sódio/uso terapêutico , Infecção da Ferida Cirúrgica/prevenção & controle , Traumatismos Abdominais/cirurgia , Adolescente , Adulto , Idoso , Anti-Infecciosos Locais/administração & dosagem , Líquido Ascítico/microbiologia , Proteína C-Reativa/análise , Calcitonina/sangue , Colectomia , Colostomia , Drenagem , Combinação de Medicamentos , Feminino , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Humanos , Ácido Hipocloroso/administração & dosagem , Perfuração Intestinal/complicações , Masculino , Metronidazol/administração & dosagem , Metronidazol/uso terapêutico , Pessoa de Meia-Idade , Peritonite/etiologia , Hipoclorito de Sódio/administração & dosagem , Ferimentos não Penetrantes/cirurgia , Adulto Jovem
14.
Am J Infect Control ; 44(9): 1044-6, 2016 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-27079244

RESUMO

Infections are the leading cause of morbidity and mortality in burn patients. Patients colonized with methicillin-resistant Staphylococcus aureus (MRSA) are at higher risk of developing an invasive infection, and MRSA is endemic in many burn units. The typical decolonization regimen of mupirocin and chlorhexidine bathing is not optimal in burn patients because of chlorhexidine limitations on nonintact skin. We studied the impact of universal decolonization using mupirocin and hypochlorous acid bathing on health care-associated MRSA infections in a burn intensive care unit. We show a significant decrease in total MRSA infections.


Assuntos
Antibacterianos/uso terapêutico , Antissepsia/métodos , Banhos/métodos , Queimaduras/complicações , Portador Sadio/tratamento farmacológico , Ácido Hipocloroso/uso terapêutico , Infecções Estafilocócicas/tratamento farmacológico , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Unidades de Queimados , Criança , Pré-Escolar , Feminino , Hospitais Comunitários , Humanos , Lactente , Unidades de Terapia Intensiva , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Staphylococcus aureus , Atenção Terciária à Saúde , Resultado do Tratamento , Adulto Jovem
15.
J Dent ; 42(3): 298-304, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24321293

RESUMO

OBJECTIVES: This study aimed to investigate deproteinizing effect of sodium-hypochlorite (NaOCl) and mild acidic hypochlorous-acid (HOCl) pretreatment on smear layer-covered dentine and to evaluate their effects on morphological characteristics of resin-dentine interface with self-etch adhesive. METHODS: Human coronal-dentine discs with standardized smear layer were pretreated with 6% NaOCl or 50ppm HOCl for 15s or 30s. Their deproteinizing effects at the treated smear layer-covered dentine surfaces were determined by the measurement of amide:phosphate ratio using ATR-FTIR analysis. In addition, using TEM, micromorphological alterations of hybridized complex and nanoleakage expression were evaluated at the interface of a self-etch adhesive (Clearfil SE Bond) to the pretreated dentine surface with or without subsequent application of a reducing agent (p-Toluenesulfinic acid salt; Accel(®)). RESULTS: Both pretreatments of NaOCl and HOCl significantly reduced the amide:phosphate ratio as compared with the no-pretreated group (p<0.05), coincident with the elimination of the hybridized smear layer on their bonded interfaces. Nanoleakage within the hybrid layer was found in the no-pretreated and NaOCl-pretreated groups, whereas the subsequent reducing agent application changed the reticular nanoleakage to spotted type. HOCl-pretreated groups showed less nanoleakage expression in a spotted pattern, regardless of reducing agent application. CONCLUSIONS: NaOCl and HOCl solutions could remove the organic component on the smear layer-covered dentine, which could eliminate the hybridized smear layer created by self-etch adhesive, leading to the reduction of nanoleakage expression within hybrid layer. CLINICAL SIGNIFICANCE: Smear layer deproteinizing could modify dentine surface, giving an appropriate substrate for bonding to self-etch adhesive system.


Assuntos
Resinas Compostas/química , Colagem Dentária , Materiais Dentários/química , Dentina/efeitos dos fármacos , Ácido Hipocloroso/uso terapêutico , Cimentos de Resina/química , Irrigantes do Canal Radicular/uso terapêutico , Camada de Esfregaço/tratamento farmacológico , Hipoclorito de Sódio/uso terapêutico , Amidas/análise , Colágeno/análise , Infiltração Dentária/classificação , Dentina/química , Humanos , Teste de Materiais , Microscopia Eletrônica de Transmissão , Fosfatos/análise , Distribuição Aleatória , Substâncias Redutoras/química , Camada de Esfregaço/patologia , Espectroscopia de Infravermelho com Transformada de Fourier , Propriedades de Superfície , Fatores de Tempo , Tolueno/análogos & derivados , Tolueno/química
16.
J Clin Invest ; 123(12): 5361-70, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24231355

RESUMO

Nuclear factor-κB (NF-κB) regulates cellular responses to inflammation and aging, and alterations in NF-κB signaling underlie the pathogenesis of multiple human diseases. Effective clinical therapeutics targeting this pathway remain unavailable. In primary human keratinocytes, we found that hypochlorite (HOCl) reversibly inhibited the expression of CCL2 and SOD2, two NF-κB-dependent genes. In cultured cells, HOCl inhibited the activity of inhibitor of NF-κB kinase (IKK), a key regulator of NF-κB activation, by oxidizing cysteine residues Cys114 and Cys115. In NF-κB reporter mice, topical HOCl reduced LPS-induced NF-κB signaling in skin. We further evaluated topical HOCl use in two mouse models of NF-κB-driven epidermal disease. For mice with acute radiation dermatitis, topical HOCl inhibited the expression of NF-κB-dependent genes, decreased disease severity, and prevented skin ulceration. In aged mice, topical HOCl attenuated age-dependent production of p16INK4a and expression of the DNA repair gene Rad50. Additionally, skin of aged HOCl-treated mice acquired enhanced epidermal thickness and proliferation, comparable to skin in juvenile animals. These data suggest that topical HOCl reduces NF-κB-mediated epidermal pathology in radiation dermatitis and skin aging through IKK modulation and motivate the exploration of HOCl use for clinical aims.


Assuntos
Regulação da Expressão Gênica/efeitos dos fármacos , Ácido Hipocloroso/uso terapêutico , NF-kappa B/fisiologia , Oxidantes/uso terapêutico , Envelhecimento da Pele/efeitos dos fármacos , Dermatopatias/tratamento farmacológico , Transportadores de Cassetes de Ligação de ATP/biossíntese , Transportadores de Cassetes de Ligação de ATP/genética , Hidrolases Anidrido Ácido , Administração Cutânea , Substituição de Aminoácidos , Animais , Células Cultivadas , Quimiocina CCL2/biossíntese , Quimiocina CCL2/genética , Inibidor p16 de Quinase Dependente de Ciclina/biossíntese , Inibidor p16 de Quinase Dependente de Ciclina/genética , Cisteína/efeitos dos fármacos , Reparo do DNA/efeitos dos fármacos , Proteínas de Ligação a DNA , Avaliação Pré-Clínica de Medicamentos , Feminino , Genes Reporter , Humanos , Ácido Hipocloroso/administração & dosagem , Quinase I-kappa B/antagonistas & inibidores , Queratinócitos/efeitos dos fármacos , Queratinócitos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , NF-kappa B/genética , Oxidantes/administração & dosagem , Oxirredução , Radiodermatite/tratamento farmacológico , Radiodermatite/patologia , Dermatopatias/genética , Dermatopatias/metabolismo , Dermatopatias/patologia , Úlcera Cutânea/prevenção & controle , Superóxido Dismutase/biossíntese , Superóxido Dismutase/genética , Transcrição Genética/efeitos dos fármacos , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/farmacologia
17.
Int J Low Extrem Wounds ; 12(2): 130-7, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23446366

RESUMO

Surgery is usually used to treat diabetic foot osteomyelitis (DFO), whether primarily or in cases in which antibiotics are not able to control infection. In many cases, the bone is only partially removed, which means that residual infection remains in the bone margins, and the wound is left open to heal by secondary intent. The use of culture-guided postoperative antibiotic treatment and adequate management of the wound must be addressed. No trials exist dealing with local treatment in the postoperative management of these cases of complicated DFO. We decided to test a super-oxidized solution, Dermacyn Wound Care (DWC; Oculus Innovative Sciences Netherlands BV, Sittard, Netherlands) to obtain preliminary experience in patients in whom infected bone remained in the surgical wounds. Our hypothesis was that DWC could be useful to control infection in the residual infected bone and surrounding soft tissues and would thus facilitate healing. Fourteen consecutive patients who underwent conservative surgery for DFO, in whom clean bone margins could not be assured, were treated in the postoperative period with DWC. Eleven cases were located in the forefoot, 6 on the first ray and the rest in lesser toes, 1 in the Lisfranc joint, and 2 on the calcaneus. No side effects appeared during treatment. Neither allergies nor skin dermatitis were found. Limb salvage was successfully achieved in 100% of the cases. Healing was achieved in a median period of 6.8 weeks.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Pé Diabético/microbiologia , Ácido Hipocloroso/uso terapêutico , Osteomielite/tratamento farmacológico , Cuidados Pós-Operatórios , Hipoclorito de Sódio/uso terapêutico , Idoso , Anti-Infecciosos Locais/farmacologia , Desbridamento , Pé Diabético/cirurgia , Combinação de Medicamentos , Feminino , Humanos , Ácido Hipocloroso/farmacologia , Salvamento de Membro , Masculino , Pessoa de Meia-Idade , Osteomielite/patologia , Osteomielite/cirurgia , Recidiva , Hipoclorito de Sódio/farmacologia , Cicatrização/efeitos dos fármacos
18.
J Contemp Dent Pract ; 13(1): 1-10, 2012 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-22430686

RESUMO

BACKGROUND: This study addresses the efficacy of an automated decontamination protocol using the germicide 'tetra acetyl ethylene diamine (TAED) perborate' (Farmec SpA, Italy). The germicide TAED perborate protocol is used in the Castellini Dental Units fitted with an Autosteril unit (an automated device that can cycle 0.26% TAED perborate solution and sterile water for cleaning the water system between patients and overnight). Prior to testing the Autosteril and the 0.26% TAED perborate protocol on the Logos Jr Dental Unit (Castellini SpA, Italy), TAED perborate was used on a dental unit water system simulation device. METHODS: A dental unit water system simulation device equipped with four dental unit water systems and with naturally grown and mature biofilm contamination was used in this study (three treatment units and one control). One treatment group used a simulated 5 minutes contact with TAED perborate and sterile water for irrigation; the second used a simulated 5 minutes contact with TAED perborate and 2 ppm ClO2 for irrigation; the third used a simulated 5 minutes contact with TAED perborate and municipal water for irrigation. The control group used municipal water for irrigation with no cleaning/disinfection protocols. This protocol was repeated for 30 cycles. Laser scanning confocal microscopy (LSCM) was used to study the effects on natural and mature biofilms, and R2A agar used to quantify heterotrophic plate counts in the effluent irrigant. Antimicrobial efficacy was evaluated by challenging TAED perborate with microbes and spores (M. smegmatis and B. subtilis). Deleterious effects of the germicide were evaluated on metal and nonmetal parts of dental unit water systems. Heterotrophic plate counts using R2A agar and LSCM of the lines were conducted to assess biofilm and microbial control. RESULTS: Baseline water samples showed mean contamination >5.6 log10 cfu/ml. After initial cleaning, all three groups maintained mean contamination levels of less than 1.1 (SD <0.3) log10 cfu/ml. LSCM of baseline samples was positive for live biofilm in all groups. At the end of the study, viable biofilm was only present in the control. In the microbial challenge test, all vegetative organisms were killed within 30 seconds of contact, while spores were killed within 5 minutes. Corrosion was seen in metals used in US-manufactured dental unit materials, while not observed in those used in the Castellini Logos Jr dental unit. CONCLUSION: In this study, the TAED perborate protocol was effective in biofilm control and control of dental treatment water contamination. Use of sterile water or 2 ppm ClO2 along with TAED treatment also controlled planktonic contamination effectively. CLINICAL SIGNIFICANCE: Environmental biofilms contaminate dental unit water systems over time and affect the quality of dental treatment water. Contaminants include environmental biofilms, microbes, including gram-negative rods and endotoxins in high doses that are not of acceptable quality for treating patients. There are many germicidal protocols for treating this contamination and one such is the prescribed use of TAED perborate used in conjunction with sterile water for irrigation in the autosteril device, an integral component of the Castellini dental units for between patient decontamination of dental unit water systems. This study was conducted on an automated simulation dental unit water system to test the TAED perborate protocol's efficacy on naturally grown, mature environmental biofilms, it's efficacy on microbes and spores and it's effects on materials used in dental unit water systems. This translational research addresses both microbial control and material effects of TAED perborate in studying efficacy and possible deleterious effects and simulated use in dentistry. Currently, this antimicrobial use protocol is followed worldwide in the Castellini dental units that are used in day-to-day dental patient care.


Assuntos
Desinfetantes de Equipamento Odontológico/uso terapêutico , Equipamentos Odontológicos/microbiologia , Etilenodiaminas/uso terapêutico , Microbiologia da Água , Purificação da Água/métodos , Abastecimento de Água , Bacillus subtilis/efeitos dos fármacos , Carga Bacteriana/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Candida albicans/efeitos dos fármacos , Corrosão , Ligas Dentárias/química , Desinfetantes de Equipamento Odontológico/administração & dosagem , Equipamentos Odontológicos/normas , Escherichia coli/efeitos dos fármacos , Etilenodiaminas/administração & dosagem , Geobacillus stearothermophilus/efeitos dos fármacos , Humanos , Ácido Hipocloroso/uso terapêutico , Viabilidade Microbiana/efeitos dos fármacos , Microscopia Confocal , Microscopia Eletrônica de Varredura , Mycobacterium smegmatis/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Esporos Bacterianos/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Fatores de Tempo
19.
Heart Surg Forum ; 13(4): E228-32, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20719724

RESUMO

BACKGROUND: Sternal wound infection is an infrequent yet potentially devastating complication following sternotomy. Among the standard practices used as preventive measures are the use of prophylactic antibiotics and povidone-iodine as an irrigation agent. A new antiseptic agent, Dermacyn super-oxidized water (Oculus Innovative Sciences), has recently been used as a wound-irrigation agent before the closure of sternotomy wounds. METHODS: This prospective, randomized clinical trial was conducted to compare the effectiveness of Dermacyn and povidone-iodine in reducing sternotomy wound infection in patients undergoing coronary artery bypass graft surgery. Upon chest closure and after insertion of sternal wires, wounds were soaked for 15 minutes with either Dermacyn or povidone-iodine. Subcutaneous tissue and skin were then closed routinely. Patients were followed up, and any wound infection was analyzed. RESULTS: Of the 178 patients, 88 patients were in the Dermacyn group, and 90 were in the povidone-iodine group. The mean (+/-SD) age of the patients was 61.1 +/- 7.6 years. The incidence of sternotomy wound infection was 19 cases (10.7%). Five (5.7%) of these cases were from the Dermacyn group, and 14 (15.6%) were from the povidone-iodine group (P = .033). No Dermacyn-related complication was identified. CONCLUSION: We found Dermacyn to be safe and more effective as a wound-irrigation agent than povidone-iodine for preventing sternotomy wound infection.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Ácido Hipocloroso/uso terapêutico , Povidona-Iodo/uso terapêutico , Hipoclorito de Sódio/uso terapêutico , Esternotomia/efeitos adversos , Esterno/cirurgia , Infecção da Ferida Cirúrgica/prevenção & controle , Irrigação Terapêutica , Idoso , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/etiologia , Comorbidade , Ponte de Artéria Coronária/métodos , Doença da Artéria Coronariana/epidemiologia , Doença da Artéria Coronariana/cirurgia , Combinação de Medicamentos , Feminino , Humanos , Incidência , Unidades de Terapia Intensiva , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Respiração Artificial/efeitos adversos , Fumar/efeitos adversos , Infecção da Ferida Cirúrgica/epidemiologia , Infecção da Ferida Cirúrgica/etiologia , Infecção da Ferida Cirúrgica/microbiologia , Fatores de Tempo , Resultado do Tratamento
20.
Adv Skin Wound Care ; 23(8): 352-7, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20664326

RESUMO

OBJECTIVE: To analyze if Vashe Wound Therapy (PuriCore, Malvern, Pennsylvania) is a valuable contribution to standard protocols of wound care. DESIGN: Open, noncomparative study. SETTING: Outpatient clinic. PATIENTS: Thirty-one patients, primarily with venous or mixed venous/arterial leg ulcers. INTERVENTIONS: Vashe Wound Therapy (hypochlorous acid, produced on site and on demand) was used as an adjunct to a standard wound care protocol. MAIN OUTCOME MEASURES: Wound healing, reduction of pain, and odor. MAIN RESULTS: At the end of the study, 86% of all lesions healed, and the average size of reduction in nonhealed wounds was 47%. Odor was present at the beginning of enrollment in 21 patients and was rated 4.58 on the visual analog scale. In all patients, the odor score at end of treatment was zero. Seventy-seven percent of all patients reported a positive pain score at the beginning of participation in the evaluation (average pain score, 4.7). At the end of the study, no patient experienced pain. CONCLUSION: Vashe Wound Therapy is a valuable contribution to standard protocols of wound care.


Assuntos
Ácido Hipocloroso/uso terapêutico , Úlcera da Perna/terapia , Higiene da Pele/métodos , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Ácido Hipocloroso/administração & dosagem , Úlcera da Perna/fisiopatologia , Úlcera da Perna/reabilitação , Masculino , Pessoa de Meia-Idade , Resultado do Tratamento , Cicatrização/fisiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...