Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 42
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
BMC Complement Altern Med ; 19(1): 113, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31159797

RESUMO

BACKGROUND: Embryonic neural stem cells (eNSCs) are immature precursors of the central nervous system (CNS), with self-renewal and multipotential differentiation capacities. These are regulated by endogenous and exogenous factors such as alpha-linolenic acid (ALA), a plant-based essential omega-3 polyunsaturated fatty acid. METHODS: In this study, we investigated the effects of various concentrations of Alyssum homolocarpum seed oil (AHSO), containing natural ALA, stearic acid (SA), myristic acid (MA), and ß-sitosterol, on proliferation and differentiation of eNSCs, in comparison to controls and to synthetic pure ALA. RESULTS: Treatment with natural AHSO (25 to 75 µM), similar to synthetic ALA, caused a significant ~ 2-fold increase in eNCSs viability, in comparison to controls. To confirm this proliferative activity, treatment of NSCs with 50 or 75 µM AHSO resulted in a significant increase in mRNA levels of notch1, hes-1 and Ki-67and NICD protein expression, in comparison to controls. Moreover, AHSO administration significantly increased the differentiation of eNSCs toward astrocytes (GFAP+) and oligodendrocytes (MBP+) in a dose dependent manner and was more potent than ALA, at similar concentrations, in comparison to controls. Indeed, only high concentrations of 100 µM AHSO, but not ALA, caused a significant increase in the frequency of neurons (ß-III Tubulin+). CONCLUSION: Our data demonstrated that AHSO, a rich source of ALA containing also other beneficial fatty acids, increased the proliferation and stimulated the differentiation of eNSCs. We suggest that AHSO's effects are caused by ß-sitosterol, SA and MA, present within this oil. AHSO could be used in diet to prevent neurodevelopmental syndromes, cognitive decline during aging, and various psychiatric disorders.


Assuntos
Brassicaceae/química , Células-Tronco Neurais/efeitos dos fármacos , Neurogênese/efeitos dos fármacos , Óleos Vegetais/farmacologia , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Antígeno Ki-67/metabolismo , Camundongos , Ácido Mirístico/análise , Células-Tronco Neurais/metabolismo , Óleos Vegetais/química , Sementes/química , Sitosteroides/análise , Ácidos Esteáricos/análise , Ácido alfa-Linoleico/análise
2.
Acta Sci Pol Technol Aliment ; 17(1): 51-58, 2018 Jan-Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29514429

RESUMO

BACKGROUND: Yogurt is a dairy product with a high nutritional value. However, like all milk products, it contains milk fat and is rich in saturated fatty acids. It would be desirable to enrich dairy products in poly- unsaturated fatty acids to increase dietary intake amongst consumers and improve their health. Also, some LAB bacteria are able to produce CLA and CLnA isomers from linoleic and linolenic acids. The aim of this study was to investigate the chemical properties and fatty acid profile of yogurt with the addition of 3.5% of rose hip seed oil. METHODS: Yogurt was made from skimmed milk and yogurt starter culture YC-180 Ch. Hansen (Denmark), with the addition of 3.5% of rose hip seed oil. The peroxide value, acid value, iodine value, TBA rate and fatty acid profile were determined in fat extracted from the yogurt after 1 and 14 days of storage and in fresh rose hip seed oil. The fatty acid profile was determined using gas chromatographic methods with mass spectrometric detectors. RESULTS: Fat extracted from the yogurts had lower levels of peroxides than the fresh oil. It was more acidic and the iodine value was higher than in the fresh oil. Rose hip seed oil enriched the product with polyunsaturated fatty acids. After 14 days of storage, linoleic and linolenic acid levels had increased. Moreover, the content of myristic and palmitic acids had decreased. CONCLUSIONS: The rose hip seed oil added to the yogurt was less susceptible to oxidation. The content of un- saturated fatty acids in the yogurt increased with the addition of the oil, making yogurt with rose hip seed oil an excellent source of Ω-3 and Ω-6 fatty acids. Conjugated linoleic (CLA) and linolenic (CLnA) acids were not detected. However, yogurt manufactured with appropriate adjunct cultures and with the correct oil addition could be a natural source of CLA and CLnA in the human diet.


Assuntos
Ácidos Graxos/análise , Óleos Vegetais/análise , Rosa/química , Sementes/química , Iogurte/análise , Dieta , Gorduras na Dieta/análise , Ácidos Graxos Ômega-3/análise , Ácidos Graxos Ômega-6/análise , Aditivos Alimentares/análise , Análise de Alimentos , Ácido Linoleico/análise , Ácido Mirístico/análise , Oxirredução , Ácido Palmítico/análise , Ácido alfa-Linoleico/análise
3.
Protein J ; 35(3): 212-7, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-27129461

RESUMO

In this study, we confirmed N-terminal myristoylation of Tetrahymena pyriformis arginine kinase (AK1) by identifying a myristoylation signal sequence at the N-terminus. A sufficient amount of modified enzyme was synthesized using an insect cell-free protein synthesis system that contains all of the elements necessary for post-transcriptional modification by fatty acids. Subsequent peptide mass fingerprinting (PMF) analyses were performed after digestion with trypsin. The PMF data covered 39 % (143 residues) of internal peptides. The target N-myristoylated peptide had a theoretical mass of 832.4477 and was clearly observed with an experimental mass (m/z-H(+)) of 832.4747. The difference between the two masses was 0.0271, supporting the accuracy of identification and indicating that the synthesized T. pyriformis AK1 is myristoylated. The fixed specimens of T. pyriformis were reacted with an anti-AK1 peptide antibody followed by a secondary antibody with a fluorescent chromophore and were observed using immunofluorescence microscope. In agreement with previous western blotting analyses, microscopic observations suggested that AK1 is localized in the cilia. The present PMF and microscopic analyses indicate that T. pyriformis AK1 may be localized and anchored to ciliary membranes via N-terminal myristoyl groups.


Assuntos
Arginina Quinase/química , Ácido Mirístico/análise , Tetrahymena pyriformis/citologia , Tetrahymena pyriformis/enzimologia , Sequência de Aminoácidos , Peptídeos/química , Tetrahymena pyriformis/química
4.
J Mol Neurosci ; 58(1): 93-101, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26445826

RESUMO

Hair analysis is with the advantage of non-invasive collection and long surveillance window. The present study employed a sensitive and reliable liquid chromatography coupled with ion trap-time of flight mass spectrometry method to study the metabonomic characters in the hair of 58 heroin abusers and 72 non-heroin abusers. Results indicated that certain endogenous metabolites, such as sorbitol and cortisol, were accelerated, and the level of arachidonic acid, glutathione, linoleic acid, and myristic acid was decreased in hair of heroin abusers. The metabonomic study is helpful for further understanding of heroin addiction and clinical diagnosis.


Assuntos
Cabelo/metabolismo , Dependência de Heroína/metabolismo , Metaboloma , Adulto , Ácido Araquidônico/análise , Estudos de Casos e Controles , Feminino , Glutationa/análise , Cabelo/química , Dependência de Heroína/diagnóstico , Humanos , Hidrocortisona/análise , Ácido Linoleico/análise , Masculino , Pessoa de Meia-Idade , Ácido Mirístico/análise , Sorbitol/análise , Detecção do Abuso de Substâncias/métodos
5.
J Oleo Sci ; 63(12): 1243-50, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25391685

RESUMO

Nowadays, data concerning the composition of Caryodendron orinocense Karst. (Euphorbiaceae) and Bactris gasipaes Kunth (Arecaceae) seed oils are lacking. In light of this fact, in this paper fatty acids and unsaponifiable fraction composition have been determined using GC-MS, HPLC-DAD (Diode Array Detector), NMR approaches and possible future applications have been preliminary investigated through estimation of antioxidant activity, performed with DPPH test. For C. orinocense linoleic acid (85.59%) was the main component, lauric (33.29%) and myristic (27.76%) acids were instead the most abundant in B. gasipaes. C. orinocense unsaponifiable fraction (8.06%) evidenced a remarkable content of ß-sitosterol, campesterol, stigmasterol, squalene and vitamin E (816 ppm). B. gasipaes revealed instead ß-sitosterol and squalene as main constituents of unsaponifiable matter (3.01%). Antioxidant capacity evidenced the best performance of C. orinocense seed oil. These preliminary results could be interesting to suggest the improvement of the population's incomes from Amazonian basin. In particular the knowledge of chemical composition of C. orinocense and B. gasipaes oils could be helpful to divulge and valorize these autochthones plants.


Assuntos
Antioxidantes , Arecaceae/química , Euphorbiaceae/química , Ácidos Graxos/isolamento & purificação , Ácidos Graxos/farmacologia , Nozes/química , Óleos Vegetais/química , Sementes/química , Colesterol/análogos & derivados , Colesterol/análise , Colesterol/isolamento & purificação , Colesterol/farmacologia , Cromatografia Líquida de Alta Pressão , Ácidos Graxos/análise , Depuradores de Radicais Livres , Cromatografia Gasosa-Espectrometria de Massas , Ácidos Láuricos/análise , Ácidos Láuricos/isolamento & purificação , Ácidos Láuricos/farmacologia , Ácido Linoleico/análise , Ácido Linoleico/isolamento & purificação , Ácido Linoleico/farmacologia , Espectroscopia de Ressonância Magnética , Ácido Mirístico/análise , Ácido Mirístico/isolamento & purificação , Ácido Mirístico/farmacologia , Fitosteróis/análise , Fitosteróis/isolamento & purificação , Fitosteróis/farmacologia , Óleos Vegetais/isolamento & purificação , Sitosteroides/análise , Sitosteroides/isolamento & purificação , Sitosteroides/farmacologia , Esqualeno/análise , Esqualeno/isolamento & purificação , Esqualeno/farmacologia , Estigmasterol/análise , Estigmasterol/isolamento & purificação , Estigmasterol/farmacologia , Vitamina E/análise , Vitamina E/isolamento & purificação , Vitamina E/farmacologia
6.
Life Sci ; 111(1-2): 53-61, 2014 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-25046734

RESUMO

AIMS: Activation of Calmodulin dependent protein kinase (CaMK)-II by exercise has a plethora of benefits in health. Fatty acids play a pivotal role in the pathogenesis of metabolic syndrome (MetS). Prevention of MetS and treatment of its main characteristics are very significant to fight against type 2 diabetes. CaMKII activation in the regulation of saturated and unsaturated fatty acids in relation to type 2 diabetes and MetS has not been studied, which became the focus of this present study. MAIN METHODS: Using Gas chromatography-Mass spectrometry, we investigated saturated fatty acids and unsaturated fatty acids. Quantitative real time PCR was also used to assess the gene expression. KEY FINDINGS: Results indicate that both palmitoleic acid and oleic acid which are monounsaturated fatty acids were increased in response to CaMKII activation. On the other hand, myristic acid and palmitic acid which are saturated fatty acids known to increase the risk factors of MetS and type 2 diabetes were decreased by exercise induction of CaMKII. Conversely, lauric acid also a saturated fatty acid was increased in response to CaMKII activation by exercise. This fatty acid is known to have beneficial effects in alleviating symptoms of both type 2 diabetes and MetS. SIGNIFICANCE: According to our knowledge, this is the first study to show that CaMKII activation by exercise regulates fatty acids essential in type 2 diabetes and MetS. CaMKII can be an avenue of designing novel therapeutic drugs in the management and treatment of type 2 diabetes and MetS.


Assuntos
Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/metabolismo , Ácidos Graxos Insaturados/análise , Ácidos Graxos/análise , Síndrome Metabólica/fisiopatologia , Condicionamento Físico Animal/fisiologia , Animais , Ativação Enzimática/fisiologia , Ácidos Graxos/fisiologia , Ácidos Graxos Monoinsaturados/análise , Ácidos Graxos Insaturados/fisiologia , Regulação Enzimológica da Expressão Gênica/fisiologia , Transportador de Glucose Tipo 4/biossíntese , Ácidos Láuricos/análise , Masculino , Músculo Esquelético/química , Ácido Mirístico/análise , Ácido Oleico/análise , Ácido Oleico/fisiologia , Ácido Palmítico/análise , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real
7.
Nat Prod Res ; 28(3): 208-11, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24354326

RESUMO

The essential oils from the cladodes of Opuntia littoralis, Opuntia ficus-indica and Opuntia prolifera growing wild on Santa Catalina Island, California, were obtained by hydrodistillation and analysed by gas chromatography-mass spectrometry (GC-MS). Terpenoids were the dominant class of volatiles in O. littoralis, with the two main components being the furanoid forms of cis-linalool oxide (10.8%) and trans-linalool oxide (8.8%). Fatty acid-derived compounds dominated the essential oil of O. ficus-indica with linoleic acid (22.3%), palmitic acid (12.7%), lauric acid (10.5%) and myristic acid (4.2%) as major fatty acids. O. prolifera oil was composed of 46.6% alkanes and the primary hydrocarbon component was heptadecane (19.2%). Sixteen compounds were common to all the three Opuntia species.


Assuntos
Opuntia/química , Óleos Vegetais/química , Alcanos/análise , California , Ácidos Graxos/análise , Ácidos Graxos/química , Cromatografia Gasosa-Espectrometria de Massas , Ácidos Láuricos/análise , Ácido Linoleico/análise , Monoterpenos/análise , Ácido Mirístico/análise , Óleos Voláteis , Opuntia/genética , Ácido Palmítico/análise , Óleos Vegetais/análise , Caules de Planta/química
8.
Andrologia ; 46(2): 194-7, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23398451

RESUMO

Seminal plasma contains various biochemical components associated with sperm function. However, there is limited information regarding the fatty acid composition of seminal plasma and their effect on sperm. The aim of this study was to identify the fatty acid content in canine seminal plasma using gas chromatography. Twelve ejaculates were studied, the seminal plasma was obtained by centrifugation and then the lipids were extracted, methylated and analysed by chromatography. The total lipids in the seminal plasma were 2.5 ± 0.3%, corresponding to 85% saturated fatty acids (SFA) and 15% unsaturated fatty acids (UFA). The greatest proportions of SFA were palmitic acid (30.4%), stearic acid (23.4%) and myristic acid (5.3%) and of UFA oleic acid (9.0%). Therefore, the protocols and techniques used enabled the identification of 18 different fatty acids in canine seminal plasma, which constitutes a good method to evaluate and quantify the fatty acid profile in this species.


Assuntos
Ácidos Graxos/análise , Sêmen/química , Animais , Cães , Ácidos Graxos Insaturados/análise , Masculino , Ácido Mirístico/análise , Ácido Oleico/análise , Ácido Palmítico/análise , Ácidos Esteáricos/análise
9.
Invest Clin ; 54(2): 171-9, 2013 Jun.
Artigo em Espanhol | MEDLINE | ID: mdl-23947006

RESUMO

We analyzed in 31 subjects, regular guests of the University food service of the Central University of Venezuela (UCVFS), in Caracas, the effects of replacing sunflower oil, commonly used in the preparation of meals, by a mix of sunflower oil and palm olein 70/30 (v/v) respectively. Plasma concentrations of total cholesterol, low and very low density lipoproteins were not changed after 40 days of the substitution. On the contrary, concentrations of high density lipoprotein and total triglycerides increased. The resistance to the oxidation of low-density lipoproteins increased considerably (p < 0.01). Today this resistance is considered as a protective factor of great importance in the prevention of the initiation of the atherogenic process. Taking into account the favorable modifications of HDL cholesterol and the clear increased resistance to the oxidation of LDL, we think that palm olein, mixed with other oils with a high ratio linoleic/palmitic (sunflower, corn, soya an the likes), can be used as a healthy alternative in human nutrition.


Assuntos
Colesterol/sangue , Gorduras Insaturadas na Dieta/farmacologia , Lipoproteínas/sangue , Óleos Vegetais/farmacologia , Adulto , HDL-Colesterol/sangue , LDL-Colesterol/sangue , VLDL-Colesterol/sangue , Gorduras Insaturadas na Dieta/administração & dosagem , Feminino , Análise de Alimentos , Humanos , Ácidos Láuricos/análise , Ácido Linoleico/análise , Masculino , Ácido Mirístico/análise , Oxirredução , Óleo de Palmeira , Ácido Palmítico/análise , Óleos Vegetais/administração & dosagem , Óleos Vegetais/química , Óleo de Girassol , Triglicerídeos/sangue , Vitamina E/análise , Adulto Jovem
10.
Invest. clín ; 54(2): 171-179, jun. 2013. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-740346

RESUMO

En 31 comensales regulares del Comedor Universitario de la Universidad Central de Venezuela (CUUCV), en Caracas. Se observó el efecto de la sustitución del aceite de girasol que se utiliza corrientemente en la preparación de las comidas en ese comedor, por un aceite obtenido de la mezcla de aceite de girasol y oleína de palma, en la proporción 70/30 (v/v) respectivamente. Después de 40 días continuos de la sustitución no hubo cambios significativos en las concentraciones de colesterol total (CT), ni del colesterol de las lipoproteínas de baja densidad (LDL) y muy baja densidad (VLDL). La concentración del colesterol de las lipoproteínas de alta densidad (HDL) aumentó significativamente (p<0,05). Los triglicéridos (TG) del plasma aumentaron en un 30%. La resistencia a la oxidación de las LDL aumentó considerablemente (p< 0,01). Hoy se considera a esta resistencia como un factor protector de gran importancia en la prevención del inicio del proceso aterogénico. Tomando en cuenta las modificaciones favorables como el aumento de colesterol de HDL sin modificación de la LDL y el claro aumento de la resistencia a la oxidación de la LDL, se considera que la oleína de palma es un aceite vegetal que puede ser utilizado sin mayores riesgos en mezcla con otros aceites que tengan una relación linoleico/palmítico más elevada como los aceites de girasol, maíz, soja y otros.


We analyzed in 31 subjects, regular guests of the University food service of the Central University of Venezuela (UCVFS), in Caracas, the effects of replacing sunflower oil, commonly used in the preparation of meals, by a mix of sunflower oil and palm olein 70/30 (v/v) respectively. Plasma concentrations of total cholesterol, low and very low density lipoproteins were not changed after 40 days of the substitution. On the contrary, concentrations of high density lipoprotein and total triglycerides increased. The resistance to the oxidation of low-density lipoproteins increased considerably (p<0, 01). Today this resistance is considered as a protective factor of great importance in the prevention of the initiation of the atherogenic process. Taking into account the favorable modifications of HDL cholesterol and the clear increased resistance to the oxidation of LDL, we think that palm olein, mixed with other oils with a high ratio linoleic/palmític (sunflower, corn, soya an the likes), can be used as a healthy alternative in human nutrition.


Assuntos
Adulto , Feminino , Humanos , Masculino , Adulto Jovem , Colesterol/sangue , Gorduras Insaturadas na Dieta/farmacologia , Lipoproteínas/sangue , Óleos Vegetais/farmacologia , HDL-Colesterol/sangue , LDL-Colesterol/sangue , VLDL-Colesterol/sangue , Gorduras Insaturadas na Dieta/administração & dosagem , Análise de Alimentos , Ácidos Láuricos/análise , Ácido Linoleico/análise , Ácido Mirístico/análise , Oxirredução , Ácido Palmítico/análise , Óleos Vegetais/administração & dosagem , Óleos Vegetais/química , Triglicerídeos/sangue , Vitamina E/análise
11.
J Dairy Sci ; 96(4): 2605-2616, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23403193

RESUMO

Milk is known to contain high concentrations of saturated fatty acids-such as palmitic (16:0), myristic (14:0), and lauric (12:0) acids-that can raise plasma cholesterol in humans, making their presence in milk undesirable. The main objective of our candidate gene study was to develop genetic markers that can be used to improve the healthfulness of bovine milk. The sterol regulatory element binding transcription factor 1 (SREBF1) known to regulate the transcription of lipogenic genes together with SREBF chaperone and insulin induced gene 1 were the candidate genes. The results showed significant association of the overall SREBF1 haplotypes with milk production and variations in lauric (12:0) and myristic (14:0) acid concentrations in milk. Haplotype H1 of SREBF1 was the most desirable to improve milk healthfulness because it was significantly associated with lower lauric (12:0) and myristic (14:0) acid concentrations compared with haplotype H3 of SREBF1, and lower lauric acid (12:0) concentration compared with haplotype H2 of SREBF1. Haplotype H1 of SREBF1, however, was significantly associated with lower milk production compared with haplotype H3 of SREBF1. We did not detect any significant associations between genetic polymorphisms in insulin induced gene 1 (INSIG1) and SREBF chaperone and milk fatty acid composition. In conclusion, genetic polymorphisms in SREBF1 can be used to develop genetic tools for the selection of animals producing milk with healthier fatty acid composition.


Assuntos
Bovinos/genética , Ácidos Graxos/análise , Leite/química , Polimorfismo Genético/genética , Proteína de Ligação a Elemento Regulador de Esterol 1/genética , Animais , Feminino , Marcadores Genéticos/genética , Haplótipos , Promoção da Saúde , Lactação/genética , Ácidos Láuricos/análise , Ácido Mirístico/análise , Polimorfismo de Nucleotídeo Único/genética , Seleção Genética
12.
Zhong Yao Cai ; 35(4): 578-81, 2012 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23019906

RESUMO

OBJECTIVE: To analyze the chemical constituents of volatile oil from the rhizomes and leaves of Pileostegia viburnoides var. glabrescens by GC-MS. METHODS: The volatile oil was extracted from the rhizomes and leaves of Pileostegia viburnoides var. glabrescens by steam distillation. The constituents of volatile oil were identified by GC-MS technology. RESULTS: 37 compounds were identified from the oil of rhizomes. 36 compounds were identified from the oil of leaves. The rhizomes and leaves volatile oil had 18 compounds in common. CONCLUSION: This study is the first one to report the volatile components of Pileostegia viburnoides var. glabrescens. It can provide a scientific basis for rational use of the rhizomes and leaves of Pileostegia viburnoides var. glabrescens.


Assuntos
Monoterpenos/análise , Óleos Voláteis/isolamento & purificação , Folhas de Planta/química , Rizoma/química , Saxifragaceae/química , Cromatografia Gasosa-Espectrometria de Massas , Ácido Mirístico/análise , Óleos Voláteis/química , Fitol/análise , Plantas Medicinais/química , Vapor
13.
ScientificWorldJournal ; 2012: 532374, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22593692

RESUMO

The physicochemical properties and fatty acid composition of Attalea dubia (Mart.) Burret (indaiá) seed oil were investigated. The oil was extracted in a soxhlet apparatus using petroleum ether and evaluated for iodine, acid, peroxide, ester, and saponification values. The oil was also analyzed using infrared and nuclear magnetic resonance spectroscopy. The fatty acid profile of the oil was determined by GC-MS. For each analysis indaiá oil was compared to Orbignya phalerata (babassu) oil. The two oils appeared to be very similar in their fatty acid composition, in which lauric acid (the most abundant), myristic acid, caprylic acid, and capric acid were the four main fatty acids detected. The unsaturated fatty acids content was lower for indaiá oil (5.8%) than for babassu oil (9.4%). The results suggest that indaiá palm tree could be cultivated as a new source of vegetable oil with potential for food and cosmetic industries.


Assuntos
Arecaceae/química , Ácidos Graxos/análise , Óleos Vegetais/análise , Sementes/química , Arecaceae/classificação , Caprilatos/análise , Ácidos Decanoicos/análise , Ácidos Graxos Insaturados/análise , Cromatografia Gasosa-Espectrometria de Massas , Concentração de Íons de Hidrogênio , Ácidos Láuricos/análise , Espectroscopia de Ressonância Magnética , Ácido Mirístico/análise , Óleos Vegetais/química , Especificidade da Espécie , Espectroscopia de Infravermelho com Transformada de Fourier
15.
Proteomics ; 10(9): 1780-93, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20213681

RESUMO

To establish a strategy for the comprehensive identification of human N-myristoylated proteins, the susceptibility of human cDNA clones to protein N-myristoylation was evaluated by metabolic labeling and MS analyses of proteins expressed in an insect cell-free protein synthesis system. One-hundred-and-forty-one cDNA clones with N-terminal Met-Gly motifs were selected as potential candidates from approximately 2000 Kazusa ORFeome project human cDNA clones, and their susceptibility to protein N-myristoylation was evaluated using fusion proteins, in which the N-terminal ten amino acid residues were fused to an epitope-tagged model protein. As a result, the products of 29 out of 141 cDNA clones were found to be effectively N-myristoylated. The metabolic labeling experiments both in an insect cell-free protein synthesis system and in the transfected COS-1 cells using full-length cDNA revealed that 27 out of 29 proteins were in fact N-myristoylated. Database searches with these 27 cDNA clones revealed that 18 out of 27 proteins are novel N-myristoylated proteins that have not been reported previously to be N-myristoylated, indicating that this strategy is useful for the comprehensive identification of human N-myristoylated proteins from human cDNA resources.


Assuntos
Ácido Mirístico/análise , Biossíntese de Proteínas , Proteínas/análise , Acilação , Sequência de Aminoácidos , Animais , Linhagem Celular , Sistema Livre de Células/química , Cercopithecus aethiops , DNA Complementar/genética , Humanos , Dados de Sequência Molecular , Peso Molecular , Ácido Mirístico/química , Ácido Mirístico/metabolismo , Proteínas/química , Proteínas/genética , Proteínas/metabolismo , Spodoptera
16.
Mol Biochem Parasitol ; 171(1): 50-4, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20138924

RESUMO

In the tsetse fly, the surface of Trypanosoma congolense parasites is covered by a dense layer of glycosylphosphatidylinositol (GPI)-anchored molecules. These include EPGENGT procyclin and protease resistant surface molecule (PRS), as well as congolense epimastigote-specific protein, CESP, and glutamic acid- and alanine-rich protein (GARP). The GPI structures of EPGENGT and GARP have been partially elucidated, but very little is known about PRS. We now purified PRS and analyzed its GPI lipid structure and carbohydrate composition using mass spectrometry. We found that unlike EPGENGT and GARP, the GPI anchor of PRS is unusually composed of inositol-acylated diacyl-phosphatidylinositols, including species containing either myristic or oleic acid at the sn-2 position of the glycerol backbone. This is the first identification of a tri-acylated GPI anchor containing myristate in procyclic form trypanosomes. In addition, we found that PRS is highly rich in galactose and sialic acid residues, suggesting that it may represent a major acceptor of the parasite trans-sialidase.


Assuntos
Membrana Celular/química , Glicoconjugados/química , Glicosilfosfatidilinositóis/análise , Ácido Mirístico/análise , Proteínas de Protozoários/química , Trypanosoma congolense/química , Trypanosoma congolense/fisiologia , Galactose/análise , Glicoconjugados/isolamento & purificação , Espectrometria de Massas , Ácido N-Acetilneuramínico/análise , Ácido Oleico/análise , Proteínas de Protozoários/isolamento & purificação
17.
Zhong Yao Cai ; 33(10): 1579-81, 2010 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-21355196

RESUMO

OBJECTIVE: To analyze and identify fatty acids from the flowers of Trollius chinensis Bunge. METHODS: To isolate and determine the constituents using GC/MS technique, quantitatively analyze their content by area normalization method. RESULTS: 31 fatty acids and 7 other constituents were isolated and determined. CONCLUSION: The major fatty acids were hexadecanoic (19.85%), (Z,Z)-9,12-octadecadienoic (14.37%), tetradecanoic (13.93%), (Z)-9-octadecenoic (13.00%), dodecanoic (6.79%), 10-hydroxy-hexadecanoic (4.37%) and octadecanoic (3.34%) acids.


Assuntos
Ácidos Graxos/análise , Flores/química , Plantas Medicinais/química , Ranunculaceae/química , Ácidos Graxos/química , Ácidos Graxos/isolamento & purificação , Cromatografia Gasosa-Espectrometria de Massas , Ácidos Láuricos/análise , Ácidos Láuricos/química , Ácidos Láuricos/isolamento & purificação , Estrutura Molecular , Peso Molecular , Ácido Mirístico/análise , Ácido Mirístico/química , Ácido Mirístico/isolamento & purificação , Ácido Palmítico/análise , Ácido Palmítico/química , Ácido Palmítico/isolamento & purificação , Ácidos Esteáricos/análise , Ácidos Esteáricos/química , Ácidos Esteáricos/isolamento & purificação
18.
Ann Nutr Metab ; 54(4): 275-82, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19641305

RESUMO

BACKGROUND: Few studies have implemented biomarkers of fatty acid intake in relation to breast cancer. AIMS: To examine possible differences in adipose tissue fatty acid composition between breast cancer patients and healthy control women. The relationship between tumor promotion and adipose tissue fatty acid synthesis was also investigated. METHODS: The study was conducted at the University of Crete. Subjects included 94 women with clinically diagnosed cancer of the breast and 131 healthy control women. Histological tumor grading and breast cancer staging were assessed. Fatty acids were determined by gas chromatography in gluteal adipose tissue. RESULTS: Conditional logistic regression analysis controlling for potential confounders indicated that elevated adipose monounsaturated fatty acids and oleic acid are associated with reduced odds of breast cancer [OR (T2 vs. T1) 0.15; 95% CI 0.03-0.64, and OR (T2 vs. T1) 0.18; 95% CI 0.04-0.71, respectively]. Adipose myristic acid was associated with an increase in breast cancer risk [OR (T3 vs. T1) 5.66; 95% CI 1.3-23.9]. CONCLUSIONS: Adipose oleic acid is inversely related, whereas adipose myristic acid is positively related to breast cancer risk. These relations could be mediated by Her-2/neu and FAS oncogenes.


Assuntos
Neoplasias da Mama/química , Ácidos Graxos/análise , Gordura Subcutânea/química , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores/análise , Neoplasias da Mama/epidemiologia , Neoplasias da Mama/patologia , Nádegas , Estudos de Casos e Controles , Feminino , Grécia , Humanos , Pessoa de Meia-Idade , Ácido Mirístico/análise , Estadiamento de Neoplasias , Ácido Oleico/análise , Fatores de Risco , Estatística como Assunto , Carga Tumoral , Adulto Jovem
19.
J Med Food ; 12(1): 203-7, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19298217

RESUMO

The chemical compositions of the essential oils obtained from leaves and flowers of Helichrysum pallasii were analyzed by gas chromatography and gas chromatography-mass spectrometry. Among the 102 identified constituents, hexadecanoic acid (16.2%), (Z,Z)-9,12-octadecadienoic acid (6.8%), tetradecanoic acid (2.6%), and (Z)-caryophyllene (4.2%) were the main constituent of the oil from leaves, while in the oil from flowers hexadecanoic acid (14.7%), (Z,Z)-9,12-octadecadienoic acid (14.2%), (Z)-caryophyllene (3.6%), and delta-cadinene (3.1%) predominated. The oils were both characterized by sesquiterpenes (33.4% for leaves and 33.7% for flowers, respectively) and fatty acids and esters (30.3% in leaves and 35% in flowers, respectively). The in vitro activity of the essential oils of the plant against some microorganisms in comparison with chloramphenicol by the broth dilution method was determined. The oils exhibited a weak activity as inhibitors of growth of Staphylococcus epidermidis in vitro (minimum inhibitory concentration = 100 microg/mL).


Assuntos
Antibacterianos/farmacologia , Helichrysum/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Óleos Vegetais/química , Anti-Inflamatórios não Esteroides/análise , Bactérias/efeitos dos fármacos , Ésteres/análise , Ácidos Graxos/análise , Ácidos Graxos Insaturados/análise , Flores , Líbano , Ácido Mirístico/análise , Ácido Palmítico/análise , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta , Óleos Vegetais/farmacologia , Sesquiterpenos/análise , Staphylococcus epidermidis/efeitos dos fármacos
20.
Carbohydr Res ; 343(17): 2971-9, 2008 Nov 24.
Artigo em Inglês | MEDLINE | ID: mdl-18804756

RESUMO

The lipooligosaccharide (LOS) from the Neisseria meningitidis prototype serogroup A strain NMA Z2491, an L9 immunotype LOS, was isolated and structurally characterized using glycosyl composition and linkage determination, mass spectrometry, and both 1- and 2-D nuclear resonance spectroscopy. The results show that the L9 LOS has an identical structure to that of an L4 LOS structure with the exception that it does not contain a sialic acid residue linked to position 3 of the lactoneotetraose terminal galactosyl residue. Further, two oligosaccharides are present in the Z2491 LOS preparation, OS1 and OS2. They differ from one another only in that OS2 contains an added glycine moiety, presumably at O-7 on the inner core Hep II residue. The structures of these oligosaccharides are as follows: where R=H or Gly.


Assuntos
Lipopolissacarídeos/química , Lipopolissacarídeos/isolamento & purificação , Neisseria meningitidis/química , Neisseria meningitidis/imunologia , Antígenos de Bactérias/química , Antígenos de Bactérias/isolamento & purificação , Sequência de Carboidratos , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Hidrólise , Ácidos Láuricos/análise , Lipopolissacarídeos/genética , Meningite Meningocócica/imunologia , Dados de Sequência Molecular , Ácido Mirístico/análise , Neisseria meningitidis/genética , Oligossacarídeos/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA