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1.
Cell Prolif ; 53(1): e12725, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31746058

RESUMO

OBJECTIVES: Activation of the sympathetic system and adrenergic ß-receptors following traumatic bone defects negatively impairs bone regeneration. Whether preventing ß-receptor activation could potentially improve bone defect repair is unknown. In this study, we investigated the effect of systematic administration and local delivery of propranolol through composite scaffolds on bone healing. MATERIALS AND METHODS: Collagen/PVA/propranolol/hydroxyapatite(CPPH)composite scaffolds were fabricated with 3D printing technique and characterized by scanning electron microscope (SEM). Micro-CT analysis and bone formation histology were performed to detect new bone formation. Osteogenic differentiation of bone marrow stromal cells (BMSCs) and osteoclastogenesis of bone marrow monocytes cultured with scaffolds extract were performed for further verification. RESULTS: Intraperitoneal injection of propranolol did not significantly improve bone repair, as indicated by micro-CT analysis and bone formation histology. However, CPPH scaffolds exhibited sustained release of propranolol in vitro and significantly enhanced bone regeneration compared with vehicle collagen/PVA/hydroxyapatite (CPH) scaffolds in vivo. Moreover, in vitro experiments indicated the scaffolds containing propranolol promoted the osteogenic differentiation and migration of rat BMSCs and inhibited osteoclastogenesis by preventing ß-receptor activation. CONCLUSIONS: This study demonstrates that local adrenergic ß-receptor blockade can effectively enhance the treatment of bone defects by stimulating osteogenic differentiation, inhibiting osteoclastogenesis and enhancing BMSCs migration.


Assuntos
Antagonistas Adrenérgicos beta/farmacologia , Células da Medula Óssea/metabolismo , Regeneração Óssea/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Propranolol/farmacologia , Tecidos Suporte/química , Antagonistas Adrenérgicos beta/química , Animais , Células da Medula Óssea/patologia , Colágeno/química , Colágeno/farmacologia , Implantes de Medicamento/farmacologia , Durapatita/química , Durapatita/farmacologia , Masculino , Álcool de Polivinil/química , Álcool de Polivinil/farmacologia , Propranolol/química , Ratos , Ratos Sprague-Dawley , Células Estromais/metabolismo , Células Estromais/patologia
2.
Food Chem Toxicol ; 135: 111048, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31830548

RESUMO

We investigated the in vitro degradation and cytotoxic effects of edible films developed from pulsed electric fields (PEF) treated zein-chitosan-poly(vinyl alcohol) dispersions at specific energy 60-70, 385-400, and 620-650 kJ/kg. The degradation was evaluated using both simulated gastro-intestinal electrolyte solutions (SGES) and enzyme hydrolysis. The results of ortho-phthaldialdehyde (OPA) test indicated that the chemical breakdown of the films in SGES and enzyme increased with degradation time, but the product's features were unmodified. The Fourier Transform Infrared spectroscopy (FTIR) data showed enhancement of zein and chitosan transformation from ordered helices to ß-sheet conformation. Relative cell survival rates of Hepa-1c1c7 cells investigated using 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) showed that the survival remained higher than 70% in both the supernatant and the residue of the SGES degraded samples and the supernatant from enzyme hydrolysis, which suggested that there was no significant toxicity of the films in the tested system. Although the residue from pancreatic digestion (240 min) (i.e. undigested films and a cocktail of digestion enzymes) expressed cytotoxicity activity, there was limited evidence of direct toxicity of the films. The findings of the study demonstrate the potential for PEF modified zein-chitosan-poly(vinyl alcohol) films as value-added biomaterials for the application in edible food packaging.


Assuntos
Biopolímeros/toxicidade , Quitosana/química , Eletricidade , Álcool de Polivinil/química , Zeína/química , Biopolímeros/química , Linhagem Celular , Proliferação de Células , Digestão , Trato Gastrointestinal/metabolismo , Humanos , Hidrólise , Técnicas In Vitro , Estrutura Molecular
3.
Carbohydr Polym ; 227: 115347, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31590845

RESUMO

Viscosupplementation, i.e. intra-articular injection of hyaluronic acid derivatives, is considered as the most effective treatment for patients with mild to moderate osteoarthritis. Even if hyaluronic acid is still considered as the gold standard, research is now focusing on the development of new products with enhanced injectability and yet reasonable viscoelastic behavior for OA treatment. A Gellan Gum (GG) hydrogel was synthesized and coated with crosslinked polyvinyl alcohol (PVA) to protect the polysaccharide from degradation during sterilization and improve its performance for the foreseen application. Thermal analyses indicated that mixed hydrogel showed a higher degree of structuring than the bare polysaccharide core without losing its swelling properties, thanks to the hydrophylicity of both coating and cross-linking agent. The PVA coating increased elastic and viscous moduli of the polysaccharide core conferring it a higher resistance to shear and compression and better thixotropic properties. Despite the double crosslinking, hydrogel was injectable. Cytocompatibility towards chondrocytes was verified.


Assuntos
Hidrogéis/química , Polissacarídeos Bacterianos/química , Álcool de Polivinil/química , Animais , Proliferação de Células , Sobrevivência Celular , Condrócitos , Módulo de Elasticidade , Humanos , Camundongos , Células NIH 3T3 , Osteoartrite/tratamento farmacológico , Viscosidade
4.
Water Res ; 168: 115152, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31614240

RESUMO

The co-existence of multiple pollutants in wastewater such as nitrate and heavy metal, is of high concern due to the potential environmental impact. In this study, a novel biomaterial PPy@Fe3O4/PVA was synthesized as a multifunctional bacteria immobilized carrier, to enhance simultaneous denitrification, Cd(II) and Mn(II) removal efficiency in bioreactor environments. The morphology and main components of the PPy@Fe3O4/PVA material were characterized by SEM and XRD. Using PPy@Fe3O4/PVA as a carrier, the maximum removal efficiencies for nitrate (0.207 mg L-1·h-1), Mn(II) (90.98%) and Cd(II) (98.78%) were increased by 27.05%, 30.27%, and 16.48%, respectively, compared to in the absence of PPy@Fe3O4/PVA. Regeneration experiments were performed, demonstrating the excellent stability and reusability of the PPy@Fe3O4/PVA material. Furthermore, effects of key factors were investigated on the performance of the PPy@Fe3O4/PVA bioreactor in simultaneous denitrification, Mn(II) and Cd(II) removal. Experimental results indicate that the highest nitrate, Mn(II) and Cd(II) removal efficiencies were obtained under the conditions of HRT of 10 h, initial Mn(II) concentration of 40 mg/L and initial Cd(II) concentration of 10 mg/L. Gas chromatography analysis indicated that N2 was the mainly final gaseous product. Moreover, the bioreactor community diversity was markedly influenced by the initial concentration of Cd(II) and Pseudomonas sp. H117 played a primary role in the process of simultaneous denitrification, Mn(II) and Cd(II) removal.


Assuntos
Álcool de Polivinil , Poluentes Químicos da Água , Materiais Biocompatíveis , Reatores Biológicos , Cádmio , Desnitrificação , Nitratos
5.
Ecotoxicol Environ Saf ; 187: 109765, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31670239

RESUMO

A novel polyvinyl alcohol/carboxymethyl cellulose/yeast double degradable hydrogel was prepared with yeast as a foaming agent. The chemical structure of the hydrogel was characterized by FTIR and XPS. The micro-structure of the hydrogel was observed by SEM. The specific surface area and pore size of hydrogel were measured by BET. Methylene blue adsorption capacity of the hydrogels were investigated and the adsorption mechanism was explored. The biodegradability of double degradable hydrogel was investigated. The results showed that yeast was encapsulated in hydrogel by electrostatic action. With the addition of yeast, not only the specific surface area and average pore size of the hydrogel increased but also methylene blue maximum adsorption capacity of the double degradable hydrogel (110 ±â€¯3.5 mg/g) was significantly higher than that of the hydrogel without yeast (57 ±â€¯1.9 mg/g). The adsorption mechanism was dominated by chemical adsorption and was accompanied by biodegradable and electrostatic adsorption. The kinetic data were fitted to the pseudo-second-order kinetic model reasonably well. The introduction of yeast promoted the biodegradable of hydrogel and increased the degradation rate of polyvinyl alcohol in the material with a maximum degradation rate of 45 ±â€¯2.8%.


Assuntos
Carboximetilcelulose Sódica/química , Hidrogéis/química , Azul de Metileno/análise , Álcool de Polivinil/química , Saccharomyces cerevisiae/química , Poluentes Químicos da Água/análise , Adsorção , Biodegradação Ambiental , Cinética , Modelos Teóricos , Porosidade , Saccharomyces cerevisiae/metabolismo , Propriedades de Superfície , Purificação da Água/métodos
6.
Int J Food Microbiol ; 312: 108375, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31669767

RESUMO

Recently, oxo-biodegradable polymers have attracted much attention due to taking less time to break down after disposal in comparison to ordinary polymers. Polyvinyl alcohol/gelatin (PVA/G) nanocomposite films, containing ZnO, TiO2 or ZnO/TiO2 nanoparticles supported on 4A zeolite (4A z), are novel active packaging that can control the release of antimicrobial compounds. The present study assessed the efficacy of PVA/G nanocomposite films with 1.5% (w/w) ZnO/4A z (treatment 1), 1.5% (w/w) TiO2/4A z (treatment 2), or 1% (w/w) ZnO, TiO2/4A z (treatment 3) in controlling the microbial load and maintaining the sensory qualities of white shrimp during storage at 4 ±â€¯1 °C. Firstly, the optimum concentration of each material for addition to the film was determined by micro-dilution and disc diffusion. Secondly, the specimens were checked for total viable count (TVC), as well as the counts of each of Pseudomonas spp., Enterobacteriaceae, Shewanella putrefaciens, inoculated Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli O157:H7. According to the results, the PVA/G nanocomposite films containing treatments 1-3 significantly decreased the number of bacteria in the treatment group in comparison to the control group (P < .05). The results of the antimicrobial activity of the three treatments by using the disc diffusion method revealed that the inhibition zone varied from 8.11 ±â€¯0.02 to 12.63 ±â€¯0.04 mm. Also it should be noted that, the finding of micro-dilution test varied from 1 ±â€¯0.01 to 3 ±â€¯0.01. The ZnO, TiO2/4A z nanocomposite had a significantly greater antimicrobial impact against Gram-negative bacteria compared to Gram-positive bacteria (P < .05). Finally, the microbiological and sensory investigation of the efficacy of the PVA/G nanocomposite films as active packaging materials revealed a considerable improvement in shrimp shelf life (12 days) in comparison to the control (6 days). Therefore, these nanocomposite films can be used as novel active packaging in the maintenance of the microbial load and sensory qualities of shrimp.


Assuntos
Antibacterianos/farmacologia , Embalagem de Alimentos/métodos , Armazenamento de Alimentos/métodos , Penaeidae/microbiologia , Titânio/farmacologia , Zeolitas/farmacologia , Óxido de Zinco/farmacologia , Animais , Carga Bacteriana/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Gelatina/farmacologia , Listeria monocytogenes/efeitos dos fármacos , Nanocompostos , Nanopartículas , Álcool de Polivinil/farmacologia , Refrigeração , Staphylococcus aureus/efeitos dos fármacos
7.
Int J Nanomedicine ; 14: 8573-8588, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31802870

RESUMO

Purpose: Impairment of wound healing is a major issue in type-2 diabetes that often causes chronic infections, eventually leading to limb and/or organ amputation. Connective tissue growth factor (CTGF) is a signaling molecule with several roles in tissue repair and regeneration including promoting cell adhesion, cell migration, cell proliferation and angiogenesis. Incorporation of CTGF in a biodegradable core-shell fiber to facilitate its sustained release is a novel approach to promote angiogenesis, cell migration and facilitate wound healing. In this paper, we report the development of CTGF encapsulated electrospun dual porous PLA-PVA core-shell fiber based membranes for diabetic wound healing applications. Methods: The membranes were fabricated by a core-shell electrospinning technique. CTGF was entrapped within the PVA core which was coated by a thin layer of PLA. The developed membranes were characterized by techniques such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD) analysis. In vitro cell culture studies using fibroblasts, keratinocytes and endothelial cells were performed to understand the effect of CTGF loaded membranes on cell proliferation, cell viability and cell migration. A chicken chorioallantoic membrane (CAM) assay was performed to determine the angiogenic potential of the membranes. Results: Results showed that the developed membranes were highly porous in morphology with secondary pore formation on the surface of individual fibers. In vitro cell culture studies demonstrated that CTGF loaded core-shell membranes improved cell viability, cell proliferation and cell migration. A sustained release of CTGF from the core-shell fibers was observed for an extended time period. Moreover, the CAM assay showed that core-shell membranes incorporated with CTGF can enhance angiogenesis. Conclusion: Owing to the excellent cell proliferation, migration and angiogenic potential of CTGF loaded core-shell PLA-PVA fibrous membranes, they can be used as an excellent wound dressing membrane for treating diabetic wounds and other chronic ulcers.


Assuntos
Fator de Crescimento do Tecido Conjuntivo/farmacologia , Diabetes Mellitus/patologia , Membranas Artificiais , Cicatrização/efeitos dos fármacos , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Endoteliais/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Humanos , Camundongos , Células NIH 3T3 , Nanofibras/química , Neovascularização Fisiológica/efeitos dos fármacos , Poliésteres/química , Álcool de Polivinil/química , Porosidade , Pele/efeitos dos fármacos , Resistência à Tração , Tecidos Suporte/química
8.
Exp Hematol ; 80: 16-20, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31874780

RESUMO

Serum albumin has long been an essential supplement for ex vivo hematopoietic and immune cell cultures. However, serum albumin medium supplements represent a major source of biological contamination in cell cultures and often cause loss of cellular function. As serum albumin exhibits significant batch-to-batch variability, it has also been blamed for causing major issues in experimental reproducibility. We recently discovered the synthetic polymer polyvinyl alcohol (PVA) as an inexpensive, Good Manufacturing Practice-compatible, and biologically inert serum albumin replacement for ex vivo hematopoietic stem cell cultures. Importantly, PVA is free of the biological contaminants that have plagued serum albumin-based media. Here, we describe that PVA can replace serum albumin in a range of blood and immune cell cultures including cell lines, primary leukemia samples, and human T lymphocytes. PVA can even replace human serum in the generation and expansion of functional chimeric antigen receptor (CAR) T cells, offering a potentially safer and more cost-efficient approach for this clinical cell therapy. In summary, PVA represents a chemically defined, biologically inert, and inexpensive alternative to serum albumin for a range of cell cultures in hematology and immunology.


Assuntos
Imunoterapia Adotiva/métodos , Álcool de Polivinil/farmacologia , Linfócitos T/efeitos dos fármacos , Animais , Técnicas de Cultura de Células , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Citotoxicidade Imunológica , Humanos , Células K562 , Leucemia Mieloide Aguda/patologia , Camundongos , Receptores de Antígenos Quiméricos , Albumina Sérica , Linfócitos T/citologia , Linfócitos T/metabolismo , Células Tumorais Cultivadas
9.
Prensa méd. argent ; 105(11): 836-841, dic2019. graf
Artigo em Inglês | LILACS, BINACIS | ID: biblio-1049996

RESUMO

Composite membrane as a flexible materials have found diverse applications in industrial and biomedical simultaneously, the recent studies have shown intrinsic improvement for membrane properties by inclusion of nanoparticles as a fillers with high portion ratio in inorganic polymers, the combination between two parts polymer and filler is as a result of collection the advantage of two component systems parts together. In this work, samples of polyvinyl alcohol (PVA)-nanoHaydroxyapatite (nHAp) composites were prepared by using casting method. The effects of addition of (nHAp) with different concentration on the optical properties of (PVA- nHAp) composite membrane have been studied by using wavelength range (220-820) nm. The absorption spectra, transmittance spectra, absorption coefficient, energy gap, refractive index, optical conductivity and extinction coefficient have been determined. The results show that the optical constants change with the increase of nHPA concentrations.


Assuntos
Álcool de Polivinil , Análise Espectral , Síntese de Produtos , Nanopartículas , Ciência dos Materiais , Hidroxiapatitas
10.
AAPS PharmSciTech ; 21(1): 6, 2019 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-31754916

RESUMO

The aim of the study is to investigate the feasibility of fabricating FDM 3D-printed gastric floating tablets with low infill percentages and the effect of infill percentage on the properties of gastric floating tablets in vitro. Propranolol hydrochloride was selected as a model drug, and drug-loaded polyvinyl alcohol (PVA) filaments were produced by hot melt extrusion (HME). Ellipsoid-shaped gastric floating tablets with low infill percentage of 15% and 25% (namely E-15 and E-25) were then prepared respectively by feeding the extruded filaments to FDM 3D printer. Thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM) were employed to characterize the filaments and 3D-printed tablets, and a series of evaluations were performed to the 3D-printed tablets, including the weight variation, drug content, hardness, in vitro floating behavior, and drug release of the tablets. The SEM results showed that the drug-loaded filaments and 3D-printed tablets appeared intact without defects, and the printed tablets were composed of filaments deposited uniformly layer by layer. The model drug and the excipients were thermally stable under the process temperature of extruding and printing, with a small amount of drug crystals dispersing in the drug-loaded filaments and 3D-printed tablets. Both E-15 and E-25 could float on artificial gastric fluids without any lag time and released in a sustained manner. Compared with E-15, the E-25 presented less weight variation, higher tablet hardness, shorter floating time, and longer drug release time.


Assuntos
Portadores de Fármacos/síntese química , Excipientes/síntese química , Impressão Tridimensional , Comprimidos/síntese química , Tecnologia Farmacêutica/métodos , Varredura Diferencial de Calorimetria/métodos , Portadores de Fármacos/farmacocinética , Liberação Controlada de Fármacos , Excipientes/farmacocinética , Álcool de Polivinil/síntese química , Álcool de Polivinil/farmacocinética , Propranolol/síntese química , Propranolol/farmacocinética , Comprimidos/farmacocinética , Difração de Raios X/métodos
11.
Biol Pharm Bull ; 42(10): 1753-1760, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31582663

RESUMO

The use of three-dimensional (3D) printing technology is expanding in various fields. The application of 3D printing is expected to increase in the pharmaceutical industry after 3D-printed tablets were approved by the U.S. Food and Drug Administration (FDA). Fused deposition modeling (FDM), a type of 3D printing, has been extensively studied for the manufacturing of tablets. A drug-loaded polymer filament, the ink of FDM 3D printers, can be prepared using the hot melt extrusion method or a simple drug-soaking method. In the present study, we investigate the influence of the experimental conditions on the loading of curcumin (model drug with fluorescence) into a polyvinylalcohol polymer filament using the soaking method. We show that organic solvent type (isopropanol, methanol, acetone, and ethanol), temperature (25 and 80°C), and drug concentration (2-333 mg/mL) greatly affect drug loading. Around 5% curcumin can be incorporated into the polyvinylalcohol filament using the soaking method. The drug dissolution from 3D-printed tablets depends on the drug content in the polymer filament. The incorporation of a higher amount of curcumin, which has poor water solubility, greatly delays drug dissolution. These results provide useful information on the preparation of 3D-printed tablets using a drug-loaded polymer filament obtained with the soaking method.


Assuntos
Impressão Tridimensional , Comprimidos/química , Tecnologia Farmacêutica/métodos , Curcumina/química , Liberação Controlada de Fármacos , Álcool de Polivinil/química , Solubilidade , Solventes/química , Temperatura Ambiente
12.
AAPS PharmSciTech ; 20(8): 322, 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31650263

RESUMO

The objectives of this work were to prepare a 5 wt% lidocaine-diclofenac ionic liquid drug-loaded gelatin/poly(vinyl alcohol) transdermal patch using a freeze/thaw method and to evaluate its physicochemical properties, in vitro release of lidocaine and diclofenac, and stability test. The lidocaine-diclofenac ionic liquid drug was produced by the ion pair reaction between the hydrochloride salts of lidocaine and the sodium salts of diclofenac. The thermal properties of the final drug product were significantly changed from the primary drugs. The ionic liquid drug could be dissolved in water and mixed in a polymer solution. The resulting transdermal patch was then exposed to 10 cycles of freezing and thawing preparation at - 20°C for 8 h and at 25°C for 4 h, respectively. As a result, it was found that the lidocaine-diclofenac ionic liquid drug-loaded transdermal patch showed good physicochemical properties and could feasibly be used in pharmaceutical applications. The lidocaine-diclofenac ionic liquid drug was not affected by the properties of the transdermal patch due to the lack of chemical interaction between polymer base and drug. The high drug release values of both lidocaine and diclofenac were controlled by the gelatin/poly(vinyl alcohol) transdermal patch. The patch showed good stability over the study period of 3 months when kept at 4°C or under ambient temperature.


Assuntos
Diclofenaco/farmacocinética , Gelatina/farmacocinética , Líquidos Iônicos/farmacocinética , Lidocaína/farmacocinética , Álcool de Polivinil/farmacocinética , Adesivo Transdérmico , Administração Cutânea , Anestésicos Locais/química , Anestésicos Locais/farmacocinética , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacocinética , Preparações de Ação Retardada/química , Preparações de Ação Retardada/farmacocinética , Diclofenaco/química , Combinação de Medicamentos , Liberação Controlada de Fármacos , Congelamento , Gelatina/química , Líquidos Iônicos/química , Lidocaína/química , Álcool de Polivinil/química
13.
BMC Cancer ; 19(1): 938, 2019 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-31601175

RESUMO

BACKGROUND: Intra-arterial therapy with embolics is established for the treatment of malignancies of the liver. However, there are no studies comparing the different effects of various embolics used in clinical practice. Herein, we analyzed the effect of 3 different embolics on tumor growth in a rat model of colorectal liver metastases. METHODS: Eight days after subcapsular implantation of 5 × 105 colorectal cancer cells (CC531) in the left liver lobe of WAG/Rij rats were randomized into 4 groups (n = 8) and underwent intra-arterial hepatic therapy. Animals received either EmboCept S®, DC Bead® or Lipiodol® Ultra-Fluid. Animals of the control group received a comparable amount of saline. Tumor growth was measured on day 8 and 11 using a three-dimensional 40 MHz ultrasound device. On day 11 tumor and liver tissue were removed for histological and immunohistochemical analyses. RESULTS: On day 11 animals of the control group showed a tumor growth of ~ 60% compared to day 8. Application of Lipiodol Ultra-Fluid® did not significantly influence tumor growth (~ 40%). In contrast, treatment with EmboCept S® or DC Bead® completely inhibited tumor growth. Of interest, application of EmboCept S® did not only completely inhibit tumor growth but even decreased tumor size. Immunohistochemical analysis showed a significant increase of necrotic areas within the tumors after application of EmboCept S® and DC Bead® compared to Lipiodol® Ultra-Fluid. CONCLUSION: The present study demonstrates that an intra-arterial therapy with EmboCept S® and DC Bead®, but not Lipiodol® Ultra-Fluid, results in a complete inhibition of rat colorectal liver metastatic growth.


Assuntos
Antineoplásicos/uso terapêutico , Neoplasias do Colo/patologia , Infusões Intra-Arteriais/métodos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/secundário , Microesferas , Álcool de Polivinil/uso terapêutico , Amido/uso terapêutico , Animais , Antineoplásicos/administração & dosagem , Antineoplásicos/efeitos adversos , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Óleo Etiodado/administração & dosagem , Óleo Etiodado/efeitos adversos , Óleo Etiodado/uso terapêutico , Feminino , Artéria Hepática , Xenoenxertos , Fígado/irrigação sanguínea , Fígado/patologia , Masculino , Modelos Animais , Necrose/patologia , Neovascularização Patológica/tratamento farmacológico , Álcool de Polivinil/administração & dosagem , Álcool de Polivinil/efeitos adversos , Ratos , Amido/administração & dosagem , Amido/efeitos adversos , Resultado do Tratamento , Carga Tumoral/efeitos dos fármacos
14.
Eur J Pharm Biopharm ; 144: 180-192, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31550525

RESUMO

Oromucosal delivery of active pharmaceutical ingredients provides an attractive alternative route of administration, due to avoidance of the first pass effect and improved patient compliance. In the current work, fused deposition modelling (FDM) 3D printing was investigated as an additive manufacturing approach for poly(vinyl alcohol)-based mucoadhesive films, enabling unidirectional drug release. For this purpose, chitosan was incorporated as a permeation and mucoadhesion enhancer whereas ethylcellulose and commercial wafer sheets were evaluated as backing layers. The formulated films were initially assessed for structural integrity and dose uniformity. Solid-state characterization of the films, including thermal methods (DSC, TGA), diffraction (XRPD) and Raman spectroscopy, was implemented to characterize the physicochemical properties of the produced polymeric filaments and buccal films. The mechanical properties of the products were investigated by instrumented indentation and tensile tests. Evaluation of buccal films was assessed in vitro, to study the effect of backing-layer type on hydration capacity of the films, diffusion of the drug throughout the restricting layer and release profiles in simulated saliva. The ex vivo performance of the manufactured products, associated with the presence of chitosan, was investigated by textural analysis for mucoadhesion properties, whereas permeation studies and histological studies were performed across porcine buccal epithelium. The results demonstrated that FDM printing is a timesaving and versatile approach in the context of manufacturing multi-layered mucoadhesive buccal films, providing unidirectional release properties.


Assuntos
Adesivos/química , Preparações Farmacêuticas/química , Administração Bucal , Animais , Celulose/análogos & derivados , Celulose/química , Quitosana/química , Composição de Medicamentos/métodos , Sistemas de Liberação de Medicamentos/métodos , Liberação Controlada de Fármacos/efeitos dos fármacos , Mucosa Bucal/metabolismo , Polímeros/química , Álcool de Polivinil/química , Impressão Tridimensional , Suínos
15.
J Environ Manage ; 251: 109618, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31563603

RESUMO

This paper aims to develop novel hydrophilic ionic liquid membranes using pervaporation for the recovery of biobutanol. Multiple polyvinyl alcohol (PVA) membranes based on three commercial ionic liquids with different loading were prepared for various experimental trials. The ionic liquids selected for the study include tributyl (tetradecyl) phosphonium chloride ([TBTDP][Cl]), tetrabutyl phosphonium bromide ([TBP][Br]) and tributyl methyl phosphonium methylsulphate ([TBMP][MS]). The synthesized membranes were characterized and tested in a custom-built pervaporation set-up. All ionic liquid membranes showed better results with total flux of 1.58 kg/m2h, 1.43 kg/m2h, 1.38 kg/m2h at 30% loading of [TBP][Br], [TBMP][MS] and [TBTDP][Cl] respectively. The comparison of ionic liquid membranes revealed that by incorporating [TBMP]MS to PVA matrix resulted in a maximum separation factor of 147 at 30 wt% loading combined with a relatively higher total flux of 1.43 kg/m2h. Density functional theory (DFT) calculations were also carried out to evaluate the experimental observations along with theoretical studies. The improved permeation properties make these phosphonium based ionic liquid a promising additive in PVA matrix for butanol-water separation under varying temperature conditions.


Assuntos
Líquidos Iônicos , Butanóis , Membranas Artificiais , Álcool de Polivinil , Água
16.
AAPS PharmSciTech ; 20(8): 310, 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31520243

RESUMO

The production of 3D-printed dosage forms requires the preparation of high-quality filaments containing an active pharmaceutical ingredient (API). The objective of this research is to prepare filaments containing dronedarone hydrochloride, a drug used in the treatment of cardiac arrhythmias. Filaments and 3D-printed tablets were subjected to characterization methods in order to prove and ensure the stability of the API and preservation of the drug content. Blends containing different proportions of dronedarone hydrochloride (DNR), polyethylene glycol (PEG), and polyvinyl alcohol filament (PVA) were prepared in two forms: as a powder mixture and as a solid dispersion. Thermogravimetric analysis was conducted, and the thermal properties of the components and polymer blends were tested using differential scanning calorimetry. Hot melt extrusion at 170 °C was used to prepare the filaments, and the fused deposition modeling technique was employed to print tablets. Drug release profiles were obtained by in vitro tests. The results indicate that the mixture containing 10 wt.% of polyethylene glycol prepared as a solid dispersion exhibits the most straightforward structure and shows only the slightest deviation from the target filament diameter. The compact structure of the tablet obtained from the filament provides a uniform in vitro drug release over a 24-h period. It also shows the smallest aberration from the expected DNR content in the tablet. The paper demonstrates that a blend containing 10 wt.% of PEG, 10 wt.% of DNR, and 80 wt.% of PVA filament is the most appropriate formula for extrusion and tablet printing.


Assuntos
Antiarrítmicos/administração & dosagem , Arritmias Cardíacas/tratamento farmacológico , Dronedarona/administração & dosagem , Antiarrítmicos/química , Dronedarona/química , Composição de Medicamentos , Liberação Controlada de Fármacos , Polietilenoglicóis , Álcool de Polivinil , Impressão Tridimensional , Solubilidade , Comprimidos
17.
Mater Sci Eng C Mater Biol Appl ; 105: 110076, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31546443

RESUMO

The composite scaffolds of bioactive glasses and polymers are often used in bone regeneration which could improve the stiffness, compressive strength and bioactivity of polymers while maintaining the osteoconductivity and osteoinductivity of bioactive glasses. But due to complicated situations and limitations of compositing process, the prepared composite materials have low uniformity and obvious phase separation, leading to problems such as poor mechanical properties and inferior new bone formation capacity. In this paper, a modified sol-gel processing technique was used to realize the homogeneous inorganic-organic composites. After hydrolysis of the metal alkoxide, the sol was mixed with the aqueous solution of polyvinyl alcohol (PVA), and through gelation and chemical reaction, the mixture was solidified into the inorganic-organic composite hydrogel. The composites showed as a uniform single phase with interpenetrating networks of PVA gel and borosilicate gel (BG) that chemically and physically interacted at the scale of molecular or nanometer, therefore PVA-BG hybrids were obtained. When immersed in phosphate-buffered saline, the PVA-BG hybrid-derived scaffolds released beneficial ions into the medium and converted to hydroxyapatite. The scaffolds were not toxic to the rat bone marrow-derived mesenchymal stem cells (rBMSCs), and supported rBMSCs proliferation. Furthermore, the alkaline phosphatase activity of the rBMSCs and the expression levels of osteogenic-related genes (alkaline phosphatase, osteocalcin and runt-related transcription factor 2) increased significantly with increasing amount of BG in the hybrid scaffolds. Finally, the bone defect repair results of critical-sized femoral condyle defect rat model demonstrated that PVA-BG hybrid scaffolds could enhance bone regeneration compared with PVA scaffolds. The results suggested that PVA-BG hybrid scaffolds may be a promising biomaterial for bone regeneration.


Assuntos
Células da Medula Óssea/metabolismo , Regeneração Óssea , Células-Tronco Mesenquimais/metabolismo , Osteogênese , Álcool de Polivinil/química , Silicatos/química , Tecidos Suporte/química , Animais , Células da Medula Óssea/citologia , Células-Tronco Mesenquimais/citologia , Ratos , Ratos Sprague-Dawley
18.
Mater Sci Eng C Mater Biol Appl ; 105: 110083, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31546466

RESUMO

Cutaneous wounds, especially chronic wounds, remain clinical challenges, and this is partially due to the complex healing process composed of four overlapping but distinct stages including hemostasis, inflammation, proliferation and remodeling. Therefore, wound dressings with spatially designed structures which can temporally regulate certain bioactive components to function at specific healing stages might be able to accelerate the healing process. In this study, nanobioglass incorporated chitosan-PVA (polyvinyl alcohol) trilayer nanofibrous membrane (nBG-TFM) was fabricated via sequential electrospinning. This membrane exhibited excellent biocompatibility, antibacterial activity and regeneration promotion effect. Furthermore, spatially designed structure optimized functions of each component and provided more suitable microenvironment as compared with uniform membrane. Rat full-thickness skin defects model and mice diabetic chronic wound model showed that nBG-TFM could achieve significantly accelerated and enhanced healing, in terms of complete re-epithelialization, improved collagen alignment and formation of skin appendages. It was revealed that nBG-TFM functioned through upregulating growth factors including VEGF and TGF-ß. Meanwhile inflammatory cytokines such as TNF-α and IL-1ß were downregulated. The technology presented in this study shed new light on designing functional wound dressings which can promote healing of chronic wounds.


Assuntos
Bandagens , Cerâmica , Quitosana , Membranas Artificiais , Nanofibras , Álcool de Polivinil , Cicatrização/efeitos dos fármacos , Ferimentos e Lesões , Animais , Linhagem Celular , Cerâmica/química , Cerâmica/farmacologia , Quitosana/química , Quitosana/farmacologia , Doença Crônica , Complicações do Diabetes/metabolismo , Complicações do Diabetes/patologia , Complicações do Diabetes/terapia , Diabetes Mellitus Experimental/metabolismo , Diabetes Mellitus Experimental/patologia , Diabetes Mellitus Experimental/terapia , Masculino , Camundongos , Nanofibras/química , Nanofibras/uso terapêutico , Álcool de Polivinil/química , Álcool de Polivinil/farmacologia , Ratos , Ratos Sprague-Dawley , Ferimentos e Lesões/metabolismo , Ferimentos e Lesões/patologia , Ferimentos e Lesões/terapia
19.
Gene ; 720: 144096, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31476405

RESUMO

Biologically active materials and polymeric materials used in tissue engineering have been one of the most attractive research areas in the past decades, especially the use of easily accessible materials from the patients that reduces or eliminates any patient's immune response. In this study, electrospun nanofibrous scaffolds were fabricated by using polyvinyl-alcohol (PVA), chitosan and hydroxyapatite (HA) polymers and platelet-rich plasma (PRP) as a bioactive substance isolated from human blood. Fabricated scaffold's structure and cytotoxicity were evaluated using scanning electron microscope and MTT assays. Scaffolds osteoinductivity was investigated by osteogenic differentiation of the mesenchymal stem cells (MSCs) at the in vitro level and then its osteoconductivity was examined by implanting at the critical-sized rat calvarial defect. The in vitro results showed that scaffolds have a good structure and good biocompatibility. Alkaline phosphatase activity, calcium content and gene expression assays were also demonstrated that their highest amount was detected in MSCs-seeded PVA-chitosan-HA(PRP) scaffold. For this reason, this scaffold alone and along with the MSCs was implanted to the animal defects. The in vivo results demonstrated that in the animals implanted with PVA-chitosan-HA(PRP), the defect was repaired to a good extent, but in those animals that received MSCs-seeded PVA-chitosan-HA(PRP), the defects was almost filled. It can be concluded that, PVA-chitosan-HA(PRP) alone or when stem cells cultured on them, has a great potential to use as an effective bone implant.


Assuntos
Diferenciação Celular , Nanofibras/química , Osteogênese , Plasma Rico em Plaquetas/química , Procedimentos Cirúrgicos Reconstrutivos , Crânio/cirurgia , Animais , Células Cultivadas , Quitosana/química , Durapatita/química , Masculino , Células-Tronco Mesenquimais/citologia , Álcool de Polivinil/química , Ratos , Ratos Sprague-Dawley , Engenharia Tecidual , Tecidos Suporte
20.
Mater Sci Eng C Mater Biol Appl ; 104: 110002, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31499949

RESUMO

Although PVA-chitosan composite hydrogel has excellent biocompatibility and antibacterial ability, its poor mechanical strength limits its application for wound dressings. Furthermore, PVA-chitosan composite hydrogel cannot satisfy the requirements of wound dressing as an environmental conditioner to accelerate wound healing. In this work, a novel lignin-chitosan-PVA composite hydrogel was prepared as wound dressing. The introduction of lignin effectively improved the mechanical strength (tensile stress is up to 46.87 MPa), protein adsorption capacity, and wound environmental regulation ability of the hydrogel. In a murine wound model, the lignin-chitosan-PVA composite hydrogel significantly accelerated wound healing. The developed hydrogel provides new opportunities for highly efficient skin wound care and management.


Assuntos
Quitosana/química , Hidrogéis/química , Hidrogéis/farmacologia , Lignina/química , Álcool de Polivinil/química , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Bandagens , Feminino , Camundongos , Pele/efeitos dos fármacos , Resistência à Tração/efeitos dos fármacos
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