Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 1.458
Filtrar
1.
ACS Appl Mater Interfaces ; 13(24): 29070-29082, 2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34101411

RESUMO

Two novel stimuli-responsive drug delivery systems (DDSs) were successfully created from bovine serum albumin- or myoglobin-gated upconversion nanoparticle-embedded mesoporous silica nanovehicles (UCNP@mSiO2) via diselenide (Se-Se)-containing linkages. More importantly, multiple roles of each scaffold of the nanovehicles were achieved. The controlled release of the encapsulated drug doxorubicin (DOX) within the mesopores was activated by triple stimuli (acidic pH, glutathione, or H2O2) of tumor microenvironments, owing to the conformation/surface charge changes in proteins or the reductive/oxidative cleavages of the Se-Se bonds. Upon release of DOX, the Förster resonance energy transfer between the UCNP cores and encapsulated DOX was eliminated, resulting in an increase in ratiometric upconversion luminescence for DOX release tracking in real time. The two protein-gated DDSs showed some differences in the drug release performances, relevant to structures and properties of the protein nanogates. The introduction of the Se-Se linkages not only increased the versatility of reductive/oxidative cleavages but also showed less cytotoxicity to all cell lines. The DOX-loaded protein-gated nanovehicles showed the inhibitory effect on tumor growth in tumor-bearing mice and negligible damage/toxicity to the normal tissues. The constructed nanovehicles in a spatiotemporally controlled manner have fascinating prospects in targeted drug delivery for cancer chemotherapy.


Assuntos
Antineoplásicos/uso terapêutico , Doxorrubicina/uso terapêutico , Portadores de Fármacos/química , Nanopartículas Metálicas/química , Neoplasias/tratamento farmacológico , Animais , Antineoplásicos/química , Bovinos , Doxorrubicina/química , Portadores de Fármacos/metabolismo , Liberação Controlada de Fármacos , Érbio/química , Feminino , Fluoretos/química , Glutationa/metabolismo , Células HeLa , Humanos , Peróxido de Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Camundongos Endogâmicos C57BL , Mioglobina/química , Mioglobina/metabolismo , Porosidade , Soroalbumina Bovina/química , Soroalbumina Bovina/metabolismo , Dióxido de Silício/química , Ensaios Antitumorais Modelo de Xenoenxerto , Itérbio/química , Ítrio/química
2.
ACS Appl Mater Interfaces ; 13(21): 24865-24876, 2021 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-34009929

RESUMO

As the use of pesticides in agriculture is increasing at an alarming rate, food contamination by pesticide residues is becoming a huge global problem. It is essential to develop a sensitive and user-friendly sensor device to quantify trace levels of pesticide and herbicide residues in food samples. Herein, we report an electrocatalyst made up of yttrium iron garnet (Y3Fe5O12; YIG) and graphitic carbon nitride (GCN) to attain picomolar-level detection sensitivity for mesotrione (MTO), which is a widely used herbicide in agriculture. First, YIG was prepared by a hydrothermal route; then, it was loaded on GCN sheets via a calcination method. The surface structures, composition, crystallinity, and interfacial and electrocatalytic properties of the YIG and YIG/GCN were analyzed. As the YIG/GCN displayed better surface and catalytic properties than YIG, YIG/GCN was modified on a screen-printed carbon electrode to fabricate a sensor for MTO. The YIG/GCN-modified electrode displayed a detection limit of 950 pM for MTO. The method was demonstrated in (spiked) fruits and vegetables. Then, the modified electrode was integrated with a miniaturized potentiostat called KAUSTat, which can be operated wirelessly by a smartphone. A first smartphone-based portable sensor was demonstrated for MTO that is suitable for use in nonlaboratory settings.


Assuntos
Cicloexanonas/análise , Compostos Férricos/química , Contaminação de Alimentos/análise , Grafite/química , Compostos de Nitrogênio/química , Praguicidas/análise , Sistemas Automatizados de Assistência Junto ao Leito , Smartphone , Ítrio/química , Técnicas Eletroquímicas , Microscopia Eletrônica de Transmissão , Reprodutibilidade dos Testes , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios X
3.
Molecules ; 26(4)2021 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-33671148

RESUMO

NaYF4:Er,Yb upconversion luminescent nanoparticles (UCNPs) were prepared by hydrothermal methods at 180 °C for 24 h. The X-ray diffraction (XRD) and TEM (transmission electron microscopy) images show that the resulting 60 nm UCNPs possess a hexagonal structure. In this work, maleic anhydride (MA) was grafted on the surface of UCNPs to induce hydrophilic properties. The photoluminescence spectra (PL) show upconversion emissions centered around 545 nm and 660 nm under excitation at 980 nm. The luminescent inks, including UCNPs@MA, polyvinyl alcohol (PVA), deionized water (DI), and ethylene glycol (EG), exhibit suitable properties for screen printing, such as high stability, emission intensity, and tunable dynamic viscosity. The printed patterns with a height of 5 mm and a width of 1.5 mm were clearly observed under the irradiation of a 980 nm laser. Our strategy provides a new route for the controlled synthesis of hydrophilic UCNPs, and shows that the UCNPs@MAs have great potential in applications of anti-counterfeiting packing.


Assuntos
Fluoretos/química , Química Verde , Tinta , Luminescência , Anidridos Maleicos/química , Nanopartículas/química , Análise Espectral , Itérbio/química , Ítrio/química , Érbio/química , Fenômenos Ópticos , Polímeros/química , Espectroscopia de Infravermelho com Transformada de Fourier , Vibração , Difração de Raios X
4.
Bioelectrochemistry ; 140: 107799, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-33774391

RESUMO

Herein, we report results of the studies relating to the fabrication of yttria-doped zirconia-reduced graphene oxide nanocomposite (nYZR) based biosensing platform for detection of salivary CYFRA-21-1 biomarker. The nYZR nanocomposite was hydrothermally synthesized and amine-functionalized using 3-aminopropyl triethoxysilane (APTES). This functionalized nanocomposite (APTES/nYZR) was electrophoretically deposited (45 V; 3 min) onto pre-hydrolyzed indium tin oxide (ITO) coated glass substrate (APTES/nYZR/ITO) followed by biofunctionalization via covalent immobilization of the anti-CYFRA-21-1 antibodies (anti-CYFRA-21-1/APTES/nYZR/ITO). The synthesized nanomaterial and the fabricated electrodes were characterized to investigate crystal structure, morphology and electrochemical properties via X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, cyclic voltammetry, differential pulse voltammetry and electrochemical impedance spectroscopy. The fabricated biosensing electrode (BSA/anti-CYFRA-21-1/APTES/nYZR/ITO) has an operating shelf life of 56 days and can be used to detect salivary CYFRA-21-1 biomarker concentration as low as 7.2 pg mL-1 with wide linear detection range of 0.01-50 ng mL-1. This work opens new opportunities to explore the electrochemical behavior of nanostructured yttria stabilized zirconia (YSZ) and its composites at room temperature and its utility in developing biosensors and other electrochemical devices.


Assuntos
Biomarcadores Tumorais/metabolismo , Técnicas Biossensoriais/métodos , Grafite/química , Neoplasias Bucais/metabolismo , Saliva/metabolismo , Ítrio/química , Zircônio/química , Humanos , Limite de Detecção , Nanocompostos/química
5.
Biomed Res Int ; 2021: 8875023, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33575352

RESUMO

Background: Monolithic restorations made of translucent yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) have become popular over the past few decades. However, whether aging affects the color and translucency of monolithic translucent Y-TZP is unclear. Objective: The aim of this systematic review and meta-analysis of in vitro studies was to evaluate the effects of aging on the color and translucency of monolithic translucent Y-TZP ceramics. Materials and Methods: This systematic review/meta-analysis was reported according to the PRISMA statement and registered in the OSF registries (https://osf.io/5qjmu). Four databases including Medline via the PubMed, Embase, and Web of Science databases and the Cochrane Library were searched using no publication year and language limits. The last search was executed on November 20, 2020. In vitro studies comparing the translucency and/or color of monolithic translucent Y-TZP ceramics before and after simulated aging were selected. Meta-analyses were performed using Review Manager software (version 5.3, Cochrane Collaboration, Oxford, UK) with random-effects models at a significance level of 0.05. A risk-of-bias assessment was also performed for the included studies. Results: Of the 188 potentially relevant studies, 13 were included in the systematic review. The hydrothermal aging duration ranged from 1 to 100 h at relatively similar temperatures (~134°C). In the general meta-analyses, the aged Y-TZP ceramics exhibited similar translucency parameter (TP), L∗, and b∗ values compared with the nonaged controls (P = .73, P = .49, and P = .62, respectively). Moreover, there was a significant difference between the aged and nonaged Y-TZP ceramics in the a∗ value (P = .03; MD = -0.26; 95% CI = -0.51 to - 0.02), favoring the nonaged Y-TZP ceramics. The subgroup analyses showed that the duration of aging contributed to changes in the translucency and color of the Y-TZP ceramics. Conclusions: The optical properties of monolithic translucent Y-TZP ceramics were stable after hydrothermal aging at 134°C and 0.2 MPa for ≤20 h. Moreover, clinically unacceptable changes in the translucency and color of monolithic translucent Y-TZP ceramics were found after hydrothermal aging for >20 h.


Assuntos
Cerâmica , Ítrio , Zircônio , Cerâmica/análise , Cerâmica/química , Cor , Teste de Materiais , Propriedades de Superfície , Temperatura , Fatores de Tempo , Ítrio/análise , Ítrio/química , Zircônio/análise , Zircônio/química
6.
Biomed Res Int ; 2021: 6612840, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33628793

RESUMO

Objective: The purpose of the study was to determine the hardness and fracture toughness of dental yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) manufactured by digital light processing (DLP) technology to study its clinical prospects. Methods: The experimental group was DLP-manufactured zirconia, and the control group was milled zirconia. The hardness was investigated under a range of test loads (0.49 N, 0.98 N, 1.96 N, 4.90 N, 9.81 N, 29.42 N, 49.03 N, 98.07 N, and 196.1 N). Meyer's law was applied to describe the indentation size effect (ISE). Meanwhile, the PSR model and MPSR model were utilized to generate true hardness values. The cracks were observed to be induced by indentation under loads above 49.03 N, while the cracks showed the radial-median type under the load of 196.1 N, under which the fracture toughness was calculated. Results: The true hardness of DLP-manufactured zirconia was 1189 HV based on the PSR model and 1193 HV based on the MPSR model, a bit lower than that of milled zirconia. The fracture toughness was 3.43 ± 0.29 MPa√m, which showed no statistical difference with the milled zirconia. Conclusion: The dental zirconia manufactured by the DLP 3D printing technique is similar to that manufactured by the conventional milling process in hardness and fracture toughness, thus having a promising future of clinical use.


Assuntos
Materiais Dentários/química , Ítrio/química , Zircônio/química , Dureza , Teste de Materiais , Microscopia Eletrônica de Varredura , Propriedades de Superfície
7.
ACS Appl Mater Interfaces ; 13(9): 10987-10995, 2021 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-33624494

RESUMO

Real-time monitoring of neurotransmitter levels is of tremendous technological demand, which requires more sensitive and selective sensors over a dynamic concentration range. As a use case, we report yttrium vanadate within three-dimensional graphene aerogel (YVO/GA) as a novel electrocatalyst for detecting dopamine (DA). This synergy effect endows YVO/GA nanocomposite with good electrochemical behaviors for DA detection compared to other electrodes. Benefiting from tailorable properties, it provides a large specific surface area, rapid electron transfer, more active sites, good catalytic activity, synergic effect, and high conductivity. The essential analytical parameters were estimated from the calibration plot, such as a limit of detection (1.5 nM) and sensitivity (7.1 µA µM-1 cm-2) with the YVO/GA sensor probe electrochemical approach. The calibration curve was fitted with the correlation coefficient of 0.994 in the DA concentration range from 0.009 to 83 µM, which is denoted as the linear working range. We further demonstrate the proposed YVO/GA sensor's applicability to detect DA in human serum sample with an acceptable recovery range. Our results imply that the developed sensor could be applied to the early analysis of dementia, psychiatric, and neurodegenerative disorders.


Assuntos
Dopamina/sangue , Grafite/química , Neurotransmissores/sangue , Vanadatos/química , Técnicas Eletroquímicas/métodos , Géis/química , Humanos , Limite de Detecção , Monitorização Fisiológica , Nanocompostos/química , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Ítrio/química
8.
ACS Appl Mater Interfaces ; 13(2): 2327-2335, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33401893

RESUMO

This study presents a controlled synthesis of NaYbF4@NaYF4 core-shell upconversion nanoparticles using the hot-injection technique. NaYF4 shells with tunable morphologies including long-rod, short-rod, and quasi-sphere are grown on identical NaYbF4 core nanoparticles by controlled injection of acetate or trifluoroacetate precursors. Mechanistic investigations reveal that anisotropic interfacial strain accounts for the preferential growth of shell layers along the c-axis. However, the strain effect can be offset by the fast injection of shell precursors, leading to nearly isotropic growth of NaYF4 shells over the NaYbF4 core nanoparticles. The core-shell nanoparticles are further modified with DNA molecules and incubated with adenocarcinomic human alveolar basal epithelial cells. Based on a combination of characterizations by flow cytometry and confocal microscopy, favorable cellular uptake and DNA delivery are observed for the quasi-sphere nanoparticles, owing to the high dispersibility and easy membrane wrapping. The method described here could be extended to synthesize other types of functional nanostructures for the study of morphology-dependent properties.


Assuntos
Portadores de Fármacos/química , Fluoretos/química , Nanopartículas/química , Itérbio/química , Ítrio/química , Células A549 , DNA/administração & dosagem , DNA/farmacocinética , Portadores de Fármacos/metabolismo , Fluoretos/metabolismo , Técnicas de Transferência de Genes , Humanos , Nanopartículas/metabolismo , Nanopartículas/ultraestrutura , Nanotecnologia , Itérbio/metabolismo , Ítrio/metabolismo
9.
Anal Bioanal Chem ; 413(5): 1363-1371, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33388932

RESUMO

Novel polyepinephrine-modified NaYF4:Yb,Tm upconversion luminescent nanoparticles (UCNP@PEP) were prepared via the self-polymerization of epinephrine on the surfaces of the UCNPs for selective sensing of Fe3+ inside a cell and for intracellular imaging. The proposed UCNP@PEP probe is a strong blue light emitter (λmax = 474 nm) upon exposure to an excitation wavelength of 980 nm. The probe was used for detecting Fe3+ owing to the complexation reaction between UCNP@PEP and Fe3+, resulting in reduced upconversion luminescence (UCL) intensity. The proposed probe has a detection limit of 0.2 µM and a good linear range of 1-10 µM for sensing Fe3+ ions. Moreover, the UCNP@PEP probe displays high cell viability (90%) and is feasible for intracellular imaging. The ability of the probe to sense Fe3+ in a human serum sample was tested and shows promising output for diagnostic purposes. The prepared UCNP@PEP probe was characterized by using UV-visible (UV-Vis) absorption spectrometry, fluorescence (FL) spectrometry, field emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FT-IR).


Assuntos
Cátions/análise , Epinefrina/química , Fluoretos/química , Ferro/análise , Nanopartículas/química , Itérbio/química , Ítrio/química , Cátions/sangue , Células HeLa , Humanos , Ferro/sangue , Luminescência , Microscopia de Fluorescência , Imagem Óptica , Polímeros/química
10.
Ecotoxicol Environ Saf ; 210: 111862, 2021 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-33429321

RESUMO

Microcystin-leucine arginine (MC-LR) is a carcinogenic toxin, produced by cyanobacteria. The release of this toxin into drinking water sources can threaten public health and environmental safety. Therefore, effective MC-LR removal from water resources is necessary. In the present study, the hydrothermal method was used to synthesize a novel ternary BiVO4/TiO2/NaY-Zeolite (B/T/N-Z) nanocomposite for MC-LR degradation under visible light. FESEM, FTIR, XRD, and DRS were performed for characterizing the nanocomposite structure. Also, the Response Surface Methodology (RSM) was applied to determine the impact of catalyst dosage, pH, and contact time on the MC-LR removal. High-performance liquid chromatography was performed to measure the MC-LR concentration. Based on the results, independent parameters, including contact time, catalyst dosage, and pH, significantly affected the MC-LR removal (P < 0.05). In other words, increasing the contact time, catalyst dosage, and acidic pH had positive effects on MC-LR removal. Among these variables, the catalyst dosage, with the mean square and F-value of 1041.37 and 162.84, respectively, had the greatest effect on the MC-LR removal efficiency. Apart from the interaction between the catalyst dosage and contact time, the interaction effects of other parameters were not significant. Also, the maximum MC-LR removal efficiency was 99.88% under optimal conditions (contact time = 120 min, catalyst dosage = 1 g/L, and pH = 5). According to the results, the B/T/N-Z nanocomposite, as a novel and effective photocatalyst could be used to degrade MC-LR from polluted water.


Assuntos
Luz , Toxinas Marinhas/química , Microcistinas/química , Nanocompostos/efeitos da radiação , Titânio/efeitos da radiação , Vanadatos/efeitos da radiação , Poluentes Químicos da Água/química , Ítrio/efeitos da radiação , Zeolitas/efeitos da radiação , Bismuto/química , Catálise , Nanocompostos/química , Processos Fotoquímicos , Titânio/química , Vanadatos/química , Purificação da Água/métodos , Ítrio/química , Zeolitas/química
11.
ACS Appl Mater Interfaces ; 13(2): 3481-3490, 2021 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-33347289

RESUMO

Nanocrystals having single-band red emission under near-infrared (NIR) excitation through the upconversion process offer great advantages in terms of enhanced cellular imaging in in vitro and in vivo experiments in the biological window (600-900 nm), as a security ink, in photothermal therapy (PTT), in photodynamic therapy (PDT), and so forth but are challenging for materials scientists. In this work, we report for the first time the preparation of a super bright red emitter at 655 nm from monodispersed NaErF4:0.5%Tm@NaYF4:20%Yb nanocrystals (core@active shell). This phosphor exhibits 35 times stronger photoluminescence as compared to NaErF4:0.5%Tm@NaYF4 (core@inactive shell). Here, an Er3+-enriched host matrix works simultaneously as an activator and a sensitizer under NIR excitation. Upconversion red emission at 655 nm arises due to the electronic transition of Er3+ via the involvement of a three-photon absorption (expected to be a two-photon absorption), which has been confirmed via a power-dependent luminescence study. Tm3+ ions incorporated into the core with the active shell act as trapping centers, which promote the red band emission via the back-energy transfer process. Moreover, the active shell containing Yb3+ ions efficiently transfers the energy to the Er3+-enriched core, which suppresses the nonradiative channel rate, and Tm3+ ions act as trapping centers, which reduce the luminescence quenching via reduction of energy migration to the surface of the host lattice. Also, we have shown the potential applications of these nanocrystals: cellular imaging through downconversion and upconversion processes and security ink.


Assuntos
Érbio/química , Fluoretos/química , Substâncias Luminescentes/química , Nanopartículas/química , Itérbio/química , Ítrio/química , Células A549 , Transferência de Energia , Humanos , Luminescência , Medições Luminescentes , Células MCF-7 , Imagem Óptica
12.
Inorg Chem ; 59(23): 17276-17281, 2020 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-33231077

RESUMO

A biocompatible Y(III)-based metal-organic framework [Y4(TATB)2]·(DMF)3.5·(H2O) (ZJU-16, H3TATB= 4,4',4''-(1,3,5-triazine-2,4,6-triyl) tribenzoic acid) was synthesized, and it was adopted to load Mn2+ for chemodynamic therapy. Meanwhile, ibuprofen sodium (IBUNa), an anti-inflammatory drug, was introduced to increase the amount of Mn2+ (about 5.66 wt %) due to the low loading capacity of Mn2+. Mn&IBUNa@ZJU-16 which was loaded by Mn2+ and IBUNa exhibited significant effects of chemodynamic therapy and excellent inhibition of the 4T1 tumor cell growth, implying its long-term prospects in chemodynamic therapy and its possibility in bimodal cancer therapy.


Assuntos
Antineoplásicos/farmacologia , Materiais Biocompatíveis/farmacologia , Neoplasias da Mama/tratamento farmacológico , Estruturas Metalorgânicas/farmacologia , Ítrio/farmacologia , Animais , Antineoplásicos/síntese química , Antineoplásicos/química , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Liberação Controlada de Fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Estruturas Metalorgânicas/síntese química , Estruturas Metalorgânicas/química , Camundongos , Células Tumorais Cultivadas , Ítrio/química
13.
Molecules ; 25(21)2020 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33138289

RESUMO

Catalysts of 10% Ni, supported on promoted alumina, were used to accomplish the partial oxidation of methane. The alumina support was doped with oxides of Mo, Mg, Ti and Y. An incipient wetness impregnation technique was used to synthesize the catalysts. The physicochemical properties of the catalysts were described by XRD, H2-TPR (temperature programmed reduction), BET, TGA, CO2-TPD (temperature-programmed desorption) and Raman. The characterization results denoted that Ni has a strong interaction with the support. The TGA investigation of spent catalysts displayed the anticoking enhancement of the promoters. The impact of the support promoters on the catalyst stability, methane conversion and H2 yield was inspected. Stability tests were done for 460 min. The H2 yields were 76 and 60% and the CH4 conversions were 67 and 92%, respectively, over Ni/Al2O3+Mg, when the reaction temperatures were 550 and 650 °C, respectively. The performance of the present work was compared to relevant findings in the literature.


Assuntos
Compostos de Alumínio/química , Manganês/química , Metano/química , Molibdênio/química , Níquel/química , Titânio/química , Ítrio/química , Catálise , Oxirredução
14.
ACS Appl Mater Interfaces ; 12(46): 51273-51284, 2020 Nov 18.
Artigo em Inglês | MEDLINE | ID: mdl-33156603

RESUMO

Neodymium-doped yttrium aluminum garnet (YAG:Nd3+) has been widely developed during roughly the past 60 years and has been an outstanding fluorescent material. It has been considered as the gold standard among multipurpose solid-state lasers. Yet, the successful downsizing of this system into the nanoregimen has been elusive, so far. Indeed, the synthesis of a garnet structure at the nanoscale, with enough crystalline quality for optical applications, was found to be quite challenging. Here, we present an improved solvothermal synthesis method producing YAG:Nd3+ nanocrystals of remarkably good structural quality. Adequate surface functionalization using asymmetric double-hydrophilic block copolymers, constituted of a metal-binding block and a neutral water-soluble block, provides stabilized YAG:Nd3+ nanocrystals with long-term colloidal stability in aqueous suspensions. These newly stabilized nanoprobes offer spectroscopic quality (long lifetimes, narrow emission lines, and large Stokes shifts) close to that of bulk YAG:Nd3+. The narrow emission lines of YAG:Nd3+ nanocrystals are exploited by differential infrared fluorescence imaging, thus achieving an autofluorescence-free in vivo readout. In addition, nanothermometry measurements, based on the ratiometric fluorescence of the stabilized YAG:Nd3+ nanocrystals, are demonstrated. The progress here reported paves the way for the implementation of this new stabilized YAG:Nd3+ system in the preclinical arena.


Assuntos
Alumínio/química , Nanopartículas/química , Neodímio/química , Imagem Óptica , Polímeros/química , Ítrio/química , Animais , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/química , Meios de Contraste/química , Lasers de Estado Sólido , Camundongos , Nanopartículas/administração & dosagem
15.
ACS Appl Mater Interfaces ; 12(47): 52976-52982, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33174736

RESUMO

A novel multichannel sensor array has been designed using a single, yet multiemissive lanthanide-doped upconversion nanoparticle (UCNP). The energy levels of lanthanide ions gave rise to several emission bands which were exploited as individual sensor elements for the recognition of four important neurotransmitters (NTs): dopamine, norepinephrine, levodopa, and serotonin. At alkaline conditions, the oxidation products of these NTs quenched the fluorescence emissions of UCNPs with different quenching degrees. The resulting fingerprint multichannel emission profiles from NaYF4:Yb/Er/Tm UCNPs allowed the discrimination of NTs with excellent accuracy. The recognition was further verified in artificial cerebrospinal fluid, as a complex biological media. We believe that the designed UCNP-based multichannel sensor array offers innovative insights into the discrimination of various chemical signatures using a single measurement.


Assuntos
Fluoretos/química , Nanopartículas Metálicas/química , Análise em Microsséries/métodos , Neurotransmissores/análise , Ítrio/química , Érbio/química , Neurotransmissores/líquido cefalorraquidiano , Espectrometria de Fluorescência , Túlio/química , Itérbio/química
16.
J Chromatogr A ; 1634: 461686, 2020 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-33220585

RESUMO

This work furthers the development of counter-current chromatography as an industrial separation process method. It was demonstrated that the industrial counter-current chromatography methods, in particular, for the separation groups of rare earth metals, can be implemented in a modified cascade of centrifugal mixer-settler extractors. The retention behavior of rare earth elements (samarium, europium, gadolinium, terbium, dysprosium and yttrium) on the pilot chromatographic unit consisting of 70 serially connected centrifugal mixer-settler extractors was experimentally studied under isocratic elution conditions using the mixture of 30 vol.% CyanexⓇ572 + 10 vol.% tributylphosphate in a hydrocarbon diluent as the stationary phase and aqueous nitric acid as the mobile phase. Theoretical analysis of experimental studies showed an acceptable agreement between the assumptions of the theory and experimental results.


Assuntos
Distribuição Contracorrente , Elementos da Série dos Lantanídeos/química , Elementos da Série dos Lantanídeos/isolamento & purificação , Disprósio/química , Disprósio/isolamento & purificação , Európio/química , Európio/isolamento & purificação , Gadolínio/química , Gadolínio/isolamento & purificação , Samário/química , Samário/isolamento & purificação , Térbio/química , Térbio/isolamento & purificação , Ítrio/química , Ítrio/isolamento & purificação
17.
Molecules ; 25(18)2020 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-32961731

RESUMO

Theranostic approach is currently among the fastest growing trends in cancer treatment. It implies the creation of multifunctional agents for simultaneous precise diagnosis and targeted impact on tumor cells. A new type of theranostic complexes was created based on NaYF4: Yb,Tm upconversion nanoparticles coated with polyethylene glycol and functionalized with the HER2-specific recombinant targeted toxin DARPin-LoPE. The obtained agents bind to HER2-overexpressing human breast adenocarcinoma cells and demonstrate selective cytotoxicity against this type of cancer cells. Using fluorescent human breast adenocarcinoma xenograft models, the possibility of intravital visualization of the UCNP-based complexes biodistribution and accumulation in tumor was demonstrated.


Assuntos
Nanopartículas Metálicas/química , Nanomedicina Teranóstica , Animais , Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Feminino , Corantes Fluorescentes/química , Fluoretos/química , Humanos , Nanopartículas Metálicas/uso terapêutico , Nanopartículas Metálicas/toxicidade , Camundongos , Camundongos Nus , Polietilenoglicóis/química , Receptor ErbB-2/metabolismo , Túlio/química , Transplante Heterólogo , Itérbio/química , Ítrio/química
18.
J Mater Chem B ; 8(40): 9251-9257, 2020 10 21.
Artigo em Inglês | MEDLINE | ID: mdl-32929430

RESUMO

The major limitations of photodynamic therapy (PDT) are the poor tissue penetration of excitation light and the neutralization of reactive oxygen species (ROS) generated by overexpressed glutathione (GSH) in cancer cells. Despite tremendous efforts to design nanoplatforms, PDT still suffers from unsatisfactory effects. Furthermore, the residual of nanomaterials in the body has restricted their clinical application. To address these issues, Janus nanocomposites containing an Yb/Er codoped NaYF4 upconverting nanocrystal head and a disulfide-bridged mesoporous organosilicon body (UCN/MON) with loaded chlorin e6 (Ce6) were designed. On one hand, the upconverting nanocrystal head can convert near-infrared (NIR) light into visible light to activate Ce6 to release ROS. On the other hand, the silica body can be degraded though a redox reaction with GSH, to not only improve the tumor selectivity of the photosensitizer by redox- and pH-triggered Ce6 release, but also diminish the concentration of GSH in cancer cells to reduce the depletion of ROS. Thereby, an enhanced PDT triggered by NIR irradiation was achieved. Furthermore, UCN/MONs showed a higher clearance rate after therapeutic actions than nonbiodegradable UCN/MSNs due to their biocompatibility. Taken together, this work revealed the potential of UCN/MONs for highly efficient and NIR-induced PDT, highlighting the prospects of UCN/MONs in the clinic.


Assuntos
Antineoplásicos/uso terapêutico , Glutationa/metabolismo , Nanocompostos/uso terapêutico , Neoplasias/tratamento farmacológico , Fármacos Fotossensibilizantes/uso terapêutico , Porfirinas/uso terapêutico , Animais , Antineoplásicos/química , Antineoplásicos/efeitos da radiação , Linhagem Celular Tumoral , Érbio/química , Érbio/efeitos da radiação , Érbio/uso terapêutico , Feminino , Fluoretos/química , Fluoretos/farmacocinética , Fluoretos/efeitos da radiação , Fluoretos/uso terapêutico , Humanos , Raios Infravermelhos , Camundongos Endogâmicos BALB C , Nanocompostos/química , Nanocompostos/efeitos da radiação , Nanopartículas/química , Nanopartículas/efeitos da radiação , Nanopartículas/uso terapêutico , Fotoquimioterapia , Fármacos Fotossensibilizantes/química , Fármacos Fotossensibilizantes/efeitos da radiação , Porfirinas/química , Porfirinas/farmacocinética , Porfirinas/efeitos da radiação , Dióxido de Silício/química , Dióxido de Silício/metabolismo , Dióxido de Silício/farmacocinética , Oxigênio Singlete/metabolismo , Itérbio/química , Itérbio/efeitos da radiação , Itérbio/uso terapêutico , Ítrio/química , Ítrio/farmacocinética , Ítrio/efeitos da radiação , Ítrio/uso terapêutico
19.
Mikrochim Acta ; 187(9): 516, 2020 08 25.
Artigo em Inglês | MEDLINE | ID: mdl-32840708

RESUMO

Aiming to the ongoing challenge of accurate and sensitive detection for cancer biomarkers, antibody-functionalized NaYF4:Yb3+, Er3+@SiO2 nanorods were developed as upconversion luminescence (UCL)-infrared absorption (IRA) nanoprobes. Benefiting from the shielding effect of the SiO2 shell, an enhanced UCL was achieved. Additionally, an IRA detection signal was introduced by the Si-O-Si bonds of SiO2. Its mutual verification with UCL signal was favorable for ensuring the accuracy of the assay. A UCL-IRA sandwich detection method was established for the detection of the prostate-specific antigen. The UCL intensity at 542 nm and IRA at 1095 cm-1 were chosen for quantitative assay. The method has high sensitivity (0.05 pg mL-1) and selectivity. The range of detection (200 fg mL-1-200 ng mL-1) was singnificantly broadened compared with that of single-readout UCL or IRA detection. The assay performance of human serum samples demonstrated the practicability of the method in clinical cancer diagnosis. Graphical abstract.


Assuntos
Nanotubos/química , Antígeno Prostático Específico/sangue , Anticorpos Imobilizados/imunologia , Érbio/química , Érbio/efeitos da radiação , Fluoretos/química , Fluoretos/efeitos da radiação , Humanos , Imunoensaio/métodos , Luz , Limite de Detecção , Luminescência , Medições Luminescentes , Nanotubos/efeitos da radiação , Antígeno Prostático Específico/imunologia , Dióxido de Silício/química , Itérbio/química , Itérbio/efeitos da radiação , Ítrio/química , Ítrio/efeitos da radiação
20.
Int J Nanomedicine ; 15: 5061-5072, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32764936

RESUMO

Purpose: Zirconia is one of the most promising implant materials due to its favorable physical, mechanical and biological properties. However, until now, we know little about the mechanism of osseointegration on zirconia. The purpose of this study is to evaluate the effect of Syndecan (Sdc) on osteoblastic cell (MC3T3-E1) adhesion and proliferation onto zirconia materials. Materials and Methods: The mirror-polished disks 15 mm in diameter and 1.5 mm in thick of commercial pure titanium (CpTi), 3mol% yttria-stabilized tetragonal zirconia polycrystalline (3Y-TZP) and nano-zirconia (NanoZr) are used in this study. MC3T3-E1 cells were seeded onto specimen surfaces and subjected to RNA interference (RNAi) for Syndecan-1, Syndecan-2, Syndecan-3, and Syndecan-4. At 48h post-transfection, the cell morphology, actin cytoskeleton, and focal adhesion were observed using scanning electron microscopy or laser scanning confocal fluorescence microscopy. At 24h and 48h post-transfection, cell counting kit-8 (CCK-8) assay was used to investigate cell proliferation. Results: The cell morphology of MC3T3-E1 cells on CpTi, 3Y-TZP, and NanoZr changed into abnormal shape after gene silencing of Syndecan. Among the Syndecan family, Sdc-2 is responsible for NanoZr-specific morphology regulation, via maintenance of cytoskeletal conformation without affecting cellular attachment. According to CCK-8 assay, Sdc-2 affects the osteoblastic cell proliferation onto NanoZr. Conclusion: Within the limitation of this study, we suggest that Syndecan affects osteoblastic cell adhesion on CpTi, 3Y-TZP, and NanoZr. Sdc-2 might be an important heparin-sensitive cell membrane regulator in osteoblastic cell adhesion, specifically on NanoZr, through the organization of actin cytoskeleton and affects osteoblastic cell proliferation.


Assuntos
Osseointegração/fisiologia , Osteoblastos/citologia , Osteoblastos/fisiologia , Sindecanas/metabolismo , Citoesqueleto de Actina/efeitos dos fármacos , Animais , Adesão Celular/fisiologia , Membrana Celular/efeitos dos fármacos , Proliferação de Células/fisiologia , Células Cultivadas , Camundongos , Microscopia Eletrônica de Varredura , Osseointegração/genética , Propriedades de Superfície , Sindecana-2/genética , Sindecana-2/metabolismo , Sindecanas/genética , Titânio/química , Ítrio/química , Zircônio/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...