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1.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3684-3694, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602940

RESUMO

Pistacia lentiscus,which belongs to foreign medicine resources,is widely distributed in the Mediterranean and Middle Eastern area. The essential oils are a mixture of several volatile compounds mainly monoterpenes and sesquiterpenes obtained from different parts of P. lentiscus by hydrodistillation. The variability of chemical composition,biological activities and content of essential oil is strongly affected by extraction technology,environmental and sex factors. It is indicated that essential oils of P. lentiscus have kinds of biological activities such as antibacterial,anticancer,anti-atherogenesis,antioxidant,anti-inflammatory and insecticidal activities.Many scholars hold the opinion that combination of different components with synergistic and/or additive actions should account for their biological activities. Due to its diverse efficacy and special taste,the essential oil of P. lentiscus has been extensively used in medicine,food and cosmetics industries. A mini review of chemical constituents and biological activities of essential oil of P. lentiscus in the past20 years is made here to provide valuable reference for the construction of " the Belt and Road".


Assuntos
Óleos Voláteis/química , Óleos Voláteis/farmacologia , Pistacia/química , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Monoterpenos/química , Monoterpenos/farmacologia , Sesquiterpenos/química , Sesquiterpenos/farmacologia
2.
Arq Bras Cir Dig ; 32(3): e1451, 2019.
Artigo em Inglês, Português | MEDLINE | ID: mdl-31644671

RESUMO

BACKGROUND: Hypovolemic shock is a common disease in polytrauma patients and may develop ischemia in various organs, increasing morbidity and mortality. The bowel is usually most affected by this condition. AIM: To evaluate the effects of copaiba oil on the intestinal mucosa's injury of rats submitted to hypovolemic shock. METHOD: Fifteen rats were divided into three groups: sham - simulated surgery; ischemia - animals submitted to hypovolemic shock; and copaiba - animals submitted to hypovolemic shock previously treated with copaiba oil. Mean blood pressure, arterial blood gas after shock induction, degree of intestinal lesion and villus length were evaluated. RESULTS: The sham presented the lowest values of lactate and PaCO2 and the highest values of mean arterial pressure, pH and bicarbonate in relation to the other groups. The degree of mesenteric lesion was zero in the sham group; 3.00±1.00 in the ischemia group; and 3.00±0.71 in the copaiba group. The villus length was 173.60±8.42 in the sham, 142.77±8.33 in the ischemia and 143.01±9.57 in the copaiba group. There was a significant difference between the sham and the other groups (p<0.05); however, there not significant difference between groups Ischemia and copaiba. CONCLUSION: Administration of copaiba oil did not reduce the intestinal mucosa lesion of rats after hypovolemic shock.


Assuntos
Anti-Inflamatórios/farmacologia , Fabaceae/química , Mucosa Intestinal/efeitos dos fármacos , Óleos Vegetais/farmacologia , Choque , Animais , Anti-Inflamatórios/química , Anti-Inflamatórios/uso terapêutico , Modelos Animais de Doenças , Íleo/patologia , Mucosa Intestinal/patologia , Isquemia/tratamento farmacológico , Masculino , Óleos Vegetais/química , Óleos Vegetais/uso terapêutico , Distribuição Aleatória , Ratos Wistar , Choque/tratamento farmacológico
3.
Rev Bras Parasitol Vet ; 28(3): 528-532, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31483029

RESUMO

The nematicidal effect of different organic materials was evaluated in order to develop a non-chemical alternative soil treatment for control of the free-living stages of small ruminant gastrointestinal nematodes. The selected organic materials were residues from the juice industry of acerola, cashew, grape, guava, papaya and pineapple, as well as castor residue from the biodiesel industry. LC90 results showed that pineapple residue was the most efficient inhibitor of larval development, followed by castor, grape, cashew, acerola, guava and papaya. Castor residue was also a good source of nitrogen and was used in a greenhouse experiment to prevent larval development in contaminated goat faeces that was deposited in pots containing the grasses Brachiaria brizantha (var. Paiaguás) or Megathyrsus maximus x M. infestum (var. Massai). Castor residue caused a significant (P < 0.05) reduction (85.04%) in Paiaguás grass contamination (L3.dry mass-1) and a reduction of 17.35% in Massai grass contamination (P > 0.05), with an increase in the biomass production of Massai (251.43%, P < 0.05) and Paiaguás (109.19%, P > 0.05) grasses. This strategy, called Econemat®, with good results in vitro shows to be promising on pasture increasing phytomass production.


Assuntos
Produção Agrícola , Sucos de Frutas e Vegetais , Nematoides/efeitos dos fármacos , Óleos Vegetais/farmacologia , Poaceae/parasitologia , Eliminação de Resíduos , Ricinus/química , Animais , Ruminantes
4.
Pestic Biochem Physiol ; 159: 59-67, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31400785

RESUMO

Phytophthora capsici is a plant oomycete pathogen, which causes many devastating diseases on a broad range of hosts. Zedoary turmeric oil (ZTO) is a kind of natural plant essential oil that has been widely used in pharmaceutical applications. However, the antifungal activity of ZTO against phytopathogens remains unknown. In this study, we found ZTO could inhibit P. capsici growth and development in vitro and in detached cucumber and Nicotiana benthamiana leaves. Besides, ZTO treatment resulted in severe damage to the cell membrane of P. capsici, leading to the leakage of intracellular contents. ZTO also induced a significant increase in relative conductivity, malondialdehyde concentration and glycerol content. Furthermore, we identified 50 volatile organic compounds from ZTO, and uncovered Curcumol, ß-elemene, curdione and curcumenol with strong inhibitory activities against mycelial growth of P. capsici. Overall, our results not only shed new light on the antifungal mechanism of ZTO, but also imply a promising alternative for the control of phytophthora blight caused by P. capsici.


Assuntos
Antifúngicos/farmacologia , Membrana Celular/efeitos dos fármacos , Óleos Voláteis/farmacologia , Phytophthora/efeitos dos fármacos , Extratos Vegetais/farmacologia , Óleos Vegetais/farmacologia , Curcuma , Phytophthora/metabolismo , Sesquiterpenos/farmacologia , Sesquiterpenos de Germacrano/farmacologia
5.
Int J Food Microbiol ; 306: 108266, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31319195

RESUMO

The antimicrobial activities of lemon oil based nanoemulsion and two different concentrations of lemon essential oil (100% and 10%) on food-brne pathogens (Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis and Salmonella Paratyphi A) and fish spoilage bacteria (Photobacterium damselae, Enterococcus faecalis, Vibrio vulnificus, Proteus mirabilis, Serratia liquefaciens, and Pseudomonas luteola) were compared in terms of disc diffusion, minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The constitutes of extracted lemon essential oil were identified by using GC-MS. Viscosity, the mean droplet size, thermodynamic stability and refractive index of nanoemulsions were determined. The main components detected in the lemon essential oil were d-limonene, p-cymene, ß-pinene with percentages of 52.85%, 14.36%, and 13.69%, respectively. It was found that lemon nanoemulsion was more effective on food-borne pathogens except K. pneumoniae than 100% lemon essential oil. 10% lemon essential oil showed the highest inhibition effect on S. Paratyphi A. The conversion of the essential oil into nanoemulsion improved antimicrobial activity. According to value of MIC, both nanoemulsion and 100% essential oil inhibited bacterial growth of all of the pathogen bacteria tested whereas they were less effective on inhibition of fish spoilage bacteria. However, 10% essential oil was more effective on spoilage bacteria than pathogens. MBC showed that nanoemulsion and 100% lemon essential oil presented a noticeable bactericidal activity against S. paratyphi A whereas 10% lemon essential oil was found as ≥25 mg/mL against pathogens and spoilage bacteria. Therefore, the use of nanoemulsion based on lemon essential oil can have potential as a natural antimicrobial agent against food-borne pathogen and spoilage bacteria for fish processing industry.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Doenças Transmitidas por Alimentos/prevenção & controle , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Citrus/classificação , Alimentos , Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/microbiologia , Cromatografia Gasosa-Espectrometria de Massas , Testes de Sensibilidade Microbiana , Extratos Vegetais/farmacologia
6.
Planta Med ; 85(13): 1054-1072, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31261421

RESUMO

The Lauraceae family is predominantly found in Asia and in the rainforests of the Americas, and consists mostly of aromatic trees. Being an essential oil producer, this family is used in the food, pharmaceutical, and cosmetic industries. This work presents a systematic review of the chemical composition and bioactivity of the essential oils from the Lauraceae family. Medline, Scielo, Web of Science, Lilacs, and Scopus were employed to identify articles published between 2000 and 2018, using "Lauraceae", "essential oil", and "biological activity" as key words. From 177 studies identified, 53 met the inclusion criteria. These studies indicated a predominance of the compounds ß-caryophyllene and 1,8-cineole in Lauraceae species, and highlighted the antioxidant, antifungal, antibacterial, and anti-inflammatory activities. Essential oils extracted from this family thus have high potential for pharmacological applications.


Assuntos
Lauraceae/química , Óleos Vegetais/farmacologia , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antifúngicos/química , Antifúngicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Óleos Vegetais/química
7.
J Oleo Sci ; 68(7): 599-606, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31178459

RESUMO

The loquat (Eriobotrya japonica) is commonly found in Japan. Its fruits are consumed raw or used in processed foods, and its leaves are used as a traditional medicine and in the manufacturing of cosmetics. Additionally, its seeds have several industrial applications. Therefore, this study aimed to estimate the fatty acid composition of loquat seed oil, and to evaluate its potential application as a deodorant. Palmitic acid, linoleic acid, behenic acid, and lignoceric acid were found to be the primary fatty acids present in the seeds, among which linoleic acid was involved in the deodorization of allyl methyl sulfide. Based on these results, loquat seed oil has potential for use in deodorant production.


Assuntos
Desodorantes , Eriobotrya/química , Ácidos Graxos/análise , Ácidos Graxos/isolamento & purificação , Ácidos Linolênicos/análise , Ácidos Linolênicos/isolamento & purificação , Ácido Palmítico/análise , Ácido Palmítico/isolamento & purificação , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Sementes/química , Compostos Alílicos , Sulfetos
8.
J Oleo Sci ; 68(7): 625-635, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31178462

RESUMO

The seed of five fruits and vegetables, which are often eaten by Chinese people, were selected as research objects to study the physicochemical properties, nutritional ingredients and antioxidant capacity of their seed oils. The fatty acid results indicated that the oleic acid was the main unsaturated fatty acid in almond oil and celery seed oil (content of 64.10% and 62.96%, respectively), and the wax gourd seed oil, watermelon seed oil and pumpkin seed oil were linoleic acid as the main unsaturated fatty acid (content of 72.45%, 76.77% and 47.35%, respectively). Unsaturated fatty acids are mainly located at the sn-2 position of the triacylglycerol (TAG), whereas saturated fatty acids are mainly located at the sn-1, 3 positions for the five seed oils. The pumpkin seed oil had certain advantages in terms of phytosterols and squalene (3716 and 2732 mg/kg, respectively). The high content of polyphenol for celery seed oil exhibits higher medicinal value. Polyphenols, and brassicasterols were have significant correlation with antioxidant capacity (p < 0.05, r = 0.890-0.998). The significant differences in nutrient composition between these fruits and vegetables seed oils indicate their unique value as food.


Assuntos
Antioxidantes , Frutas/química , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Verduras/química , Fenômenos Químicos , China , Colestadienóis , Ácidos Graxos/análise , Ácidos Graxos/química , Ácidos Graxos/isolamento & purificação , Ácidos Graxos Insaturados/análise , Ácidos Graxos Insaturados/química , Ácidos Graxos Insaturados/isolamento & purificação , Ácidos Linoleicos/análise , Ácidos Linoleicos/isolamento & purificação , Ácido Oleico/análise , Ácido Oleico/isolamento & purificação , Fitosteróis , Polifenóis/análise , Polifenóis/isolamento & purificação , Esqualeno , Triglicerídeos/química
9.
BMC Complement Altern Med ; 19(1): 146, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31227024

RESUMO

BACKGROUND: There is increasing interest in the pharmaceutical and food industries to substitute synthetic chemicals with naturally occurring compounds possessing bioactive properties. Plants are valuable sources of bioactive compounds. The present study investigates the chemical composition and antioxidant, antimicrobial, and anticancer activities of ethanolic extracts (EEs) and essential oils (EOs) from two species in the Lamiaceae family, Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut., cultivated in the Algerian Saharan Atlas. METHODS: The total flavonoid contents of the plants' ethanolic extracts were determined by the aluminium chloride method, while the total phenols were determined using the Folin-Ciocalteu method. Essential oils were obtained by hydrodistillation of the aerial parts of the plants and were analysed by GC-MS. The free radical-scavenging ability and antioxidant potential of the plants' EEs and EOs were probed using the 2, 2-diphenyl-picrylhydrazyl radical-scavenging, ABTS radical-scavenging, ferric-reducing power and phosphomolybdenum assays. The antimicrobial activities were evaluated against several pathogens characteristic of gram-negative bacteria (three species), gram-positive bacteria (three species) and fungi (two species). The microdilution method was used to estimate the minimum inhibitory concentrations (MICs). The oils' anticancer potential against several cancer types was also studied using the MTT assay and reported as the toxic doses that resulted in a 50% reduction in cancer cell growth (LD50). RESULTS: Phenolic compounds in the EEs from both plants were analysed by HPLC and demonstrated a rich flavonoid content. Chemical analysis of the essential oil from Ocimum basilicum revealed 26 unique compounds, with linalool (52.1%) and linalyl acetate (19.1%) as the major compounds. A total of 29 compounds were identified in the essential oil from Thymus algeriensis, with α-terpinyl acetate (47.4%), neryl acetate (9.6%), and α-pinene (6.8%) as the major compounds. The ethanolic extracts and essential oils from both plants exhibited moderate antioxidant activities and moderate to weak antimicrobial activities. Furthermore, anticancer activities against the examined human cancer cell lines were associated with only the EOs from both plants, with LD50 values ranging between 300 and 1000 µg/mL. CONCLUSION: The results suggest that the bioactive compounds found in the ethanolic extracts and essential oils from Ocimum basilicum and Thymus algeriensis, with diverse antioxidant, antimicrobial and anticancer activities, may have beneficial applications in nutraceutical and pharmaceutical technologies.


Assuntos
Ocimum basilicum/química , Óleos Voláteis/química , Óleos Vegetais/química , Thymus (Planta)/química , Argélia , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Linhagem Celular Tumoral , Sobrevivência Celular , Cromatografia Líquida de Alta Pressão , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Humanos , Fenol/química , Fenol/farmacologia , Óleos Vegetais/farmacologia
10.
BMC Complement Altern Med ; 19(1): 113, 2019 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-31159797

RESUMO

BACKGROUND: Embryonic neural stem cells (eNSCs) are immature precursors of the central nervous system (CNS), with self-renewal and multipotential differentiation capacities. These are regulated by endogenous and exogenous factors such as alpha-linolenic acid (ALA), a plant-based essential omega-3 polyunsaturated fatty acid. METHODS: In this study, we investigated the effects of various concentrations of Alyssum homolocarpum seed oil (AHSO), containing natural ALA, stearic acid (SA), myristic acid (MA), and ß-sitosterol, on proliferation and differentiation of eNSCs, in comparison to controls and to synthetic pure ALA. RESULTS: Treatment with natural AHSO (25 to 75 µM), similar to synthetic ALA, caused a significant ~ 2-fold increase in eNCSs viability, in comparison to controls. To confirm this proliferative activity, treatment of NSCs with 50 or 75 µM AHSO resulted in a significant increase in mRNA levels of notch1, hes-1 and Ki-67and NICD protein expression, in comparison to controls. Moreover, AHSO administration significantly increased the differentiation of eNSCs toward astrocytes (GFAP+) and oligodendrocytes (MBP+) in a dose dependent manner and was more potent than ALA, at similar concentrations, in comparison to controls. Indeed, only high concentrations of 100 µM AHSO, but not ALA, caused a significant increase in the frequency of neurons (ß-III Tubulin+). CONCLUSION: Our data demonstrated that AHSO, a rich source of ALA containing also other beneficial fatty acids, increased the proliferation and stimulated the differentiation of eNSCs. We suggest that AHSO's effects are caused by ß-sitosterol, SA and MA, present within this oil. AHSO could be used in diet to prevent neurodevelopmental syndromes, cognitive decline during aging, and various psychiatric disorders.


Assuntos
Brassicaceae/química , Células-Tronco Neurais/efeitos dos fármacos , Neurogênese/efeitos dos fármacos , Óleos Vegetais/farmacologia , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Antígeno Ki-67/metabolismo , Camundongos , Ácido Mirístico/análise , Células-Tronco Neurais/metabolismo , Óleos Vegetais/química , Sementes/química , Sitosteroides/análise , Ácidos Esteáricos/análise , Ácido alfa-Linoleico/análise
11.
Am J Chin Med ; 47(4): 727-750, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31094213

RESUMO

Cancer management is a worldwide challenge. In addition to effective cancer therapies like chemotherapy, radiotherapy and surgery, treatment based on traditional Chinese medicine (TCM) and combined TCM with western medicine has gradually gained attention in Oriental countries. One potential TCM approach using extracted fatty oils, containing fatty acids which are important active ingredients with a variety of pharmacological activities, makes significant contributions to cancer treatment. The strategies of treating cancer with the fatty oils of TCM were classified into "Fuzheng", which usually associates with improving immunity, represented by coix seed oil. The other classification is "Quxie", which relates to inducing apoptosis of cancer cells, and is represented by Brucea javanica oil. Compared with other active substances, the literature about anticancer fatty oils is relatively limited, and most of them focus on the composition and other biological activities without a systematic review. Therefore, based on the theories of "Fuzheng" and "Quxie" in TCM, in this paper, the anticancer effects of fatty oils have been reviewed. The chemical composition, anticancer mechanism, listed drugs, studying dosage form and clinical application of fatty oils have also been discussed. In summary, since there are different types and abundance of fatty oils among botanicals, anticancer effects of fatty oils can be achieved through two TCM theory-based strategies. We hoped that this review paper can reveal the anticancer potential of fatty oils and provide a reference for future related studies.


Assuntos
Medicamentos de Ervas Chinesas/química , Ácidos Graxos/uso terapêutico , Glicerol/uso terapêutico , Medicina Tradicional Chinesa , Neoplasias/tratamento farmacológico , Óleos Vegetais/química , Óleos Vegetais/uso terapêutico , Antineoplásicos Fitogênicos , Apoptose/efeitos dos fármacos , Ácidos Graxos/química , Ácidos Graxos/isolamento & purificação , Ácidos Graxos/farmacologia , Glicerol/química , Glicerol/isolamento & purificação , Glicerol/farmacologia , Humanos , Neoplasias/imunologia , Neoplasias/patologia , Fitoterapia , Óleos Vegetais/isolamento & purificação , Óleos Vegetais/farmacologia
12.
J Anim Sci ; 97(7): 2901-2913, 2019 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-31119279

RESUMO

The thyme (Thymus vulgaris) essential oil was assessed as antibiotic growth promoter replacement in quail chick diet and in vitro test. In total, 250-d-old Japanese quail chicks (mixed sex) were allocated into 5 dietary treatments of 5 replications (6 females and 4 males in each cage with the size of 40× 90× 25 cm) under a completely randomized design. The dietary treatments were included the control diet, control diet without any additive, control diet plus 100 ppm flavophospholipol as an antibiotic growth promoter, control diet plus 200 ppm TVE, control diet plus 300 ppm TVE, and control diet plus 400 ppm T. vulgaris essential (TVE) oil. Feed intake, BW gain, feed conversion ratio (FCR), organs weight, morphology of intestine, serum lipids, and microbial population were measured on day 35. Lipid oxidation of stored muscle tissue was measured by TBARS test. GC-MS assay, DPPH method, and well diffusion method were evaluated for determination of components, antioxidant, and antimicrobial properties, respectively. FCR improved significantly in 400 ppm TVE compared with 200 and 300 ppm TVE (P < 0.05). The serum triglyceride decreased significantly in both sexes receiving 400 ppm TVE compared with control. Villi height increased significantly in duodenum accompanied by decreasing crypt dept at all TVE levels compared with control and antibiotic. The breast muscle tissue of quail fed on 300 and 400 ppm TVE reduced the rate of oxidation during refrigerated storage compared with control. Thymol was the main component (35.40%) of the thymus oil. The considerable antioxidant activity of TVE was identified by IC50 of 58.48 µg/mL. Moreover, zones of growth inhibition of Gram-positive bacteria and Escherichia coli were numerically greater in different doses of TVE than antibiotics. Therefore, The TVE is suitable alternative component for antibiotic growth promoters by dosing consideration. However, it is possible that antibiotic resistance would increase for these natural compounds along the time.


Assuntos
Antioxidantes/metabolismo , Coturnix/fisiologia , Suplementos Nutricionais/análise , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Thymus (Planta)/química , Ração Animal/análise , Animais , Antibacterianos/farmacologia , Dieta/veterinária , Feminino , Intestinos/efeitos dos fármacos , Intestinos/microbiologia , Masculino , Distribuição Aleatória , Timol/metabolismo
13.
J Sci Food Agric ; 99(11): 5168-5175, 2019 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-31056749

RESUMO

BACKGROUND: It is well known that plant essential oils have good antimicrobial activity. However, their strong volatility and intense odor limit their application. Mesoporous silica (MCM-41), a non-toxic mesoporous material with excellent loading capability, is a promising delivery system for different types of food ingredients in the food industry. RESULTS: In this study, we first performed component analysis of pepper fragrant essential oil (PFEO) by gas chromatography - mass spectrometry (GC-MS), then the MCM-41 host was prepared, and the essential oil functionalized nanoparticles (EONs) were formed by embedding PFEO into mesoporous silica particles. Further analysis indicated that the particle size and zeta potential of EONs were 717 ± 13.38 nm and - 43.90 ± 0.67 mV, respectively. Transmission electron microscopy (TEM) images showed that EONs had an inerratic morphology and stable structure. The bactericidal activities of PFEO and EONs against Escherichia coli (E. coli), Salmonella enterica (S. enterica), Staphylococcus aureus (S. aureus) and Listeria monocytogenes (L. monocytogenes) were subsequently tested using the twofold dilution method. Results indicated that, after 48 h incubation, minimum bactericidal concentrations (MBC) of EONs used against gram-negative bacteria were decreased to a greater degree than those of PFEO, suggesting that nanoencapsulation by MCM-41 can improve antimicrobial activity. Atomic force microscopy (AFM) observation also confirmed that EONs showed a notable inhibitory effect against E. coli by disrupting cell membrane structure. CONCLUSION: Pepper fragrant essential oil nanoencapsulation could be a very promising organic delivery system in food industry for antimicrobial activity enhancement. © 2019 Society of Chemical Industry.


Assuntos
Antibacterianos/farmacologia , Nanopartículas/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Antibacterianos/química , Portadores de Fármacos/química , Composição de Medicamentos , Escherichia coli/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Listeria monocytogenes/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Odorantes/análise , Dióxido de Silício/química , Staphylococcus aureus/efeitos dos fármacos
14.
Zhongguo Zhong Yao Za Zhi ; 44(7): 1357-1362, 2019 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-31090292

RESUMO

In this study, solid dispersion technology was used to develop volatile oil from Acorus tatarinowii self-nanoemulsion dropping pills(VOA-SNEDDS-DP) and its protective effect on acute myocardial ischemia injury was evaluated. Taking exterior quality, weight variation and the resolving time as comprehendsive evaluation indexes, the preparation process and formulation of the dropping pills were optimized by orthogonal design, and the dissolution rate in vitro of the optimized VOA-SNEDDS-DP was investigated. The rat model of acute myocardial ischemia was induced by intraperitoneal injection of isoproterenol hydrochloride and the serum levels of superoxide dismutase(SOD), malondialdehyde(MDA), creatine kinase(CK) and pathological changes of myocardial tissue were determined to evaluate therapeutic effect of the dropping pills on acute myocardial ischemia. The results showed that the optimal formulation and preparation process of VOA-SNEDDS-DP were as follows: PEG6000-PEG8000 was 1∶1, proportion of VOA-SNEDDS and matrix was l∶2.5, the temperature of drug fluids was 75 ℃, drop rate was 35 drops/min, drop distance was 5 cm, the condensing agent temperature was 2-10 ℃. The content of ß-asarone in the dropping pills was 42.46 mg·g~(-1). The accumulated dissolution rate of the dropping pills reached 93.85% in 10 min. The results of pharmacodynamic experiments showed that VOA-SNEDDS-DP could significantly increase the SOD content(P<0.05), reduce the levels of MDA and CK(P<0.05) in serum, and effectively improve the pathological morphology of myocardial tissue. These results revealed that the preparation of VOA-SNEDDS-DP by solid dispersion technology was stable and feasible, and VOA-SNEDDS-DP had protective effect on acute myocardial ischemia injury.


Assuntos
Acorus/química , Medicamentos de Ervas Chinesas/farmacologia , Isquemia Miocárdica/tratamento farmacológico , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Animais , Creatina Quinase/sangue , Malondialdeído/sangue , Ratos , Superóxido Dismutase/sangue
15.
J Food Sci ; 84(6): 1427-1438, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-31070787

RESUMO

Thyme essential oil-loaded microcapsules (TMS) were fabricated using natural polysaccharide chitosan (CS) and sodium alginate (SA) as the shell material via the method of layer-by-layer (LBL) assembly. The accumulated release rates of thyme oil and microcapsules at 4 °C were 42.50% and 10.16%, respectively. After heating at 100 °C for 5 hr, the release rate of the 0, 2, 4, 6 layers assembled microcapsules were 100%, 48.84%, 28.38%, 19.3%, severally. Microcapsules also had good pH sensitivity in the range of 4 to 10. Antimicrobial function studies showed that the microcapsules are more effective than thyme oil for three tested microorganisms. When the temperature rose from 37 °C to 121 °C, the antibacterial zone of thyme oil gradually decreased from 18.5 ± 0.6 mm to 12.3 ± 0.6 mm, although inhibition rate of microcapsules increased from 87.97% to 99.75%. The antibacterial effect of thyme oil declined with the increase of pH, in terms of microcapsules, the efficiency was better under acidic or alkaline conditions. The thyme oil microcapsules can suppress the growth of Staphylococcus aureus in milk and prolong its shelf life. It was determined that this microcapsule could be a potential alternative as a natural antimicrobial agent in food and pharmaceutical industries. PRACTICAL APPLICATION: This work provided release performance and mechanism of layer-by-layer (LBL) thyme oil microcapsule under different conditions, and further studies showed its antibacterial ability to explore how herb essential oils can be potentially applied in food packaging and antibacterial areas.


Assuntos
Óleos Vegetais , Thymus (Planta)/química , Alginatos/química , Animais , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Cápsulas/química , Cápsulas/farmacologia , Quitosana/química , Preparações de Ação Retardada , Concentração de Íons de Hidrogênio , Leite/microbiologia , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Temperatura Ambiente
16.
Pol J Vet Sci ; 22(1): 173-175, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30997781

RESUMO

The aim of this study was to investigate interactions between conventional antifungal drug and essential oils against isolates of Malassezia pachydermatis. Antifungal activity of Cinnamomum cassia, Melaleuca alternifolia, Mentha piperita, Origanum vulgare and Syzygium aromaticum essential oils were tested against 19 strains of M. pachydermatis isolated from healthy dogs and reference strain M. pachydermatis CBS 1879. The checkerboard assay was used to search for in- teractions. Synergism was observed for the combination of clotrimazole with Melaleuca alternifolia essential oil, Mentha piperita and Origanum vulgare. The combinations of Cinnamomum cassia and Syzygium aromaticum essential oils with clotrimazole showed indifferent effect. Additive antimicrobial activity was observed for the combination of clotrimazole with Syzygium aromaticum and Melaleuca alternifolia essential oils against reference strain. The obtained results showed synergistic interactions between essential oils and clotrimazole which could improve effectiveness of this antifungal drug.


Assuntos
Antifúngicos/farmacologia , Clotrimazol/farmacologia , Malassezia/efeitos dos fármacos , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Antifúngicos/administração & dosagem , Clotrimazol/administração & dosagem , Óleos Voláteis/administração & dosagem , Óleos Voláteis/química , Óleos Vegetais/administração & dosagem
17.
Cell Mol Biol (Noisy-le-grand) ; 65(3): 89-93, 2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30942160

RESUMO

In this study, 1% and 2% of macerated fenugreek oil was added to the feeds of rainbow trout with an average weight of 25.79 ± 1.5 g. At the end of the study, growth rate, blood parameters and NBT (Nitroblue Tetrazolium) level of rainbow trout were determined. The best feed ratio (FCR) was observed in the control group (0.77). Statistically significant differences were found only in MID values (P<0.05), although there was a numerical increase in all blood parameters. There was no statistically significant difference between NBT levels (P> 0.05). Although the best weight gain was in the control group as in the FCR values, the maximum elongation was measured at D1 and then at D2 (P <0.05). The best survival rate was obtained with 96.66% in D1 while the worst was observed in D2 with 60% (p<0,05).


Assuntos
Ração Animal , Nitroazul de Tetrazólio/metabolismo , Oncorhynchus mykiss/anatomia & histologia , Oncorhynchus mykiss/sangue , Óleos Vegetais/farmacologia , Trigonella/química , Animais , Comportamento Alimentar , Análise de Sobrevida
18.
Rev Soc Bras Med Trop ; 52: e20180197, 2019 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-30994796

RESUMO

INTRODUCTION: Aedes aegypti and Aedes albopictus are important vectors that transmit arboviruses to human populations. METHODS: Natural products were obtained and tested against larvae collected from the field in Fortaleza, capital of Ceará state. RESULTS: The essential oils of Syzygium aromaticum (Ae. aegypti LC50 = 32.7 ppm and Ae. albopictus LC50 = 138.1 ppm) and Croton nepetaefolius (Ae. aegypti LC50 = 81.7 ppm and Ae. albopictus LC50 = 76.1 ppm) showed the most intense larvicidal activity. CONCLUSIONS: The essential oils and methyl esters showed greater larvicidal activity than did the ethanol extracts.


Assuntos
Aedes/efeitos dos fármacos , Inseticidas/farmacologia , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Animais , Inseticidas/isolamento & purificação , Larva/efeitos dos fármacos , Dose Letal Mediana , Óleos Voláteis/classificação , Óleos Voláteis/isolamento & purificação , Óleos Vegetais/classificação , Óleos Vegetais/isolamento & purificação
19.
Artif Cells Nanomed Biotechnol ; 47(1): 1404-1415, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30945563

RESUMO

Hypericum perforatum oil loaded electrospun polymeric wound dressing material was produced in order to be used in wound therapy. H. perforatum oil is known to have curative effect on wound-healing process. Wound dressing material was produced in two layers, upper layer of which was made of electrospun PCL nanofibres in order to maintain membrane integrity and mechanical strength, and bottom layer that is designed to be in contact with the wound was formed by electrospraying and electrospining of PEG/H. perforatum oil and PCL polymer solutions from opposite directions (concurrently). Methods such as FTIR, optical and electron microscopy, tensile tests, gas permeability tests, contact angle and swelling tests, in vitro release tests were utilized for material characterization. Encapsulation of H. perforatum oil in PEG capsules which were hold by PEG fibres among PCL fibres was confirmed. H. perforatum oil was released in controlled manner. Antimicrobial activity tests on Staphylococcus aureus and Escherichia coli revealed that H. perforatum content exhibited antimicrobial activity on both. Material was found to be biocompatible and suitable for use as wound dressing according to the results of in vitro tests, in which L929 mouse fibroblast cell line incubated with materials for investigation of biocompatibility (WST-1) and cell-material interactions (proliferation, apoptosis/necrosis).


Assuntos
Bandagens/microbiologia , Hypericum/química , Membranas Artificiais , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Cicatrização/efeitos dos fármacos , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Apoptose/efeitos dos fármacos , Linhagem Celular , Escherichia coli/efeitos dos fármacos , Interações Hidrofóbicas e Hidrofílicas , Camundongos , Permeabilidade , Polietilenoglicóis/química , Porosidade , Staphylococcus aureus/efeitos dos fármacos
20.
Int J Mol Sci ; 20(7)2019 Apr 09.
Artigo em Inglês | MEDLINE | ID: mdl-30970626

RESUMO

Naturally-occurring mixtures of phytochemicals present in plant foods are proposed to possess tumor-suppressive activities. In this work, we aimed to evaluate the antitumor effects of Thymus vulgaris L. in in vivo and in vitro mammary carcinoma models. Dried T. vulgaris (as haulm) was continuously administered at two concentrations of 0.1% and 1% in the diet in a chemically-induced rat mammary carcinomas model and a syngeneic 4T1 mouse model. After autopsy, histopathological and molecular analyses of rodent mammary carcinomas were performed. In addition, in vitro evaluations using MCF-7 and MDA-MB-231 cells were carried out. In mice, T. vulgaris at both doses reduced the volume of 4T1 tumors by 85% (0.1%) and 84% (1%) compared to the control, respectively. Moreover, treated tumors showed a substantial decrease in necrosis/tumor area ratio and mitotic activity index. In the rat model, T. vulgaris (1%) decreased the tumor frequency by 53% compared to the control. Analysis of the mechanisms of anticancer action included well-described and validated diagnostic and prognostic markers that are used in both clinical approach and preclinical research. In this regard, the analyses of treated rat carcinoma cells showed a CD44 and ALDH1A1 expression decrease and Bax expression increase. Malondialdehyde (MDA) levels and VEGFR-2 expression were decreased in rat carcinomas in both the T. vulgaris treated groups. Regarding the evaluations of epigenetic changes in rat tumors, we found a decrease in the lysine methylation status of H3K4me3 in both treated groups (H3K9m3, H4K20m3, and H4K16ac were not changed); up-regulations of miR22, miR34a, and miR210 expressions (only at higher doses); and significant reductions in the methylation status of four gene promoters-ATM serin/threonine kinase, also known as the NPAT gene (ATM); Ras-association domain family 1, isoform A (RASSF1); phosphatase and tensin homolog (PTEN); and tissue inhibitor of metalloproteinase-3 (TIMP3) (the paired-like homeodomain transcription factor (PITX2) promoter was not changed). In vitro study revealed the antiproliferative and proapoptotic effects of essential oils of T. vulgaris in MCF-7 and MDA-MB-231 cells (analyses of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS); 5-bromo-20-deoxyuridine (BrdU); cell cycle; annexin V/PI; caspase-3/7; Bcl-2; PARP; and mitochondrial membrane potential). T. vulgaris L. demonstrated significant chemopreventive and therapeutic activities against experimental breast carcinoma.


Assuntos
Neoplasias da Mama/tratamento farmacológico , Óleos Voláteis/administração & dosagem , Óleos Vegetais/administração & dosagem , Thymus (Planta)/química , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular , Relação Dose-Resposta a Droga , Epigênese Genética/efeitos dos fármacos , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Células MCF-7 , Camundongos , Óleos Voláteis/farmacologia , Fitoterapia , Óleos Vegetais/farmacologia , Ratos , Ensaios Antitumorais Modelo de Xenoenxerto
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