Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 5.211
Filtrar
1.
Zhongguo Zhong Yao Za Zhi ; 44(17): 3684-3694, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31602940

RESUMO

Pistacia lentiscus,which belongs to foreign medicine resources,is widely distributed in the Mediterranean and Middle Eastern area. The essential oils are a mixture of several volatile compounds mainly monoterpenes and sesquiterpenes obtained from different parts of P. lentiscus by hydrodistillation. The variability of chemical composition,biological activities and content of essential oil is strongly affected by extraction technology,environmental and sex factors. It is indicated that essential oils of P. lentiscus have kinds of biological activities such as antibacterial,anticancer,anti-atherogenesis,antioxidant,anti-inflammatory and insecticidal activities.Many scholars hold the opinion that combination of different components with synergistic and/or additive actions should account for their biological activities. Due to its diverse efficacy and special taste,the essential oil of P. lentiscus has been extensively used in medicine,food and cosmetics industries. A mini review of chemical constituents and biological activities of essential oil of P. lentiscus in the past20 years is made here to provide valuable reference for the construction of " the Belt and Road".


Assuntos
Óleos Voláteis/química , Óleos Voláteis/farmacologia , Pistacia/química , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Monoterpenos/química , Monoterpenos/farmacologia , Sesquiterpenos/química , Sesquiterpenos/farmacologia
2.
Rev Bras Parasitol Vet ; 28(3): 522-527, 2019 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-31483036

RESUMO

The anthelmintic resistance stimulated the search for strategies for controlling gastrointestinal nematodes, including the use of free essential oils or its nanoemulsion. This study evaluated the anthelmintic efficacy of Cymbopogon citratus essential oil (CcEO) and C. citratus essential oil nanoemulsion (CcEOn). Pysicochemical analyses were performed. The in vitro effect was determined using the egg hatch test (EHT) on Haemonchus contortus and in vivo effect was evaluated in sheep infected with gastrointestinal nematodes. The animals were treated with CcEO (500 mg/kg) or CcEOn (450 mg/kg) for the fecal egg count (FEC) and the determination of worm burden. The main component of CcEO was citral. The CcEO content in the nanoemulsion was 20% (v/v), and the mean particle size was 248 nm. In EHT, CcEO and CcEOn (1.25 mg/mL) inhibited larval hatching by 98.4 and 97.1%, respectively. Three animals treated with CcEO died whereas in the group treated with CcEOn one animal died. The FEC and total worm burden of the treated groups did not differ from the negative control (p>0.05). The CcEOn showed efficacy only on H. contortus (p<0.05). In conclusion, nanoencapsulation reduced toxicity and increased efficacy on H. contortus.


Assuntos
Anti-Helmínticos/farmacologia , Cymbopogon/química , Nematoides/efeitos dos fármacos , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Doenças dos Ovinos/parasitologia , Animais , Fezes/parasitologia , Feminino , Masculino , Nematoides/classificação , Nematoides/isolamento & purificação , Ovinos
3.
Medicine (Baltimore) ; 98(36): e17009, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31490383

RESUMO

Erythrina corallodendron L., a kind of landscape tree, has long been used as a traditional medicine. In this study, the composition of essential oil extracted from the leaves was analysed by GC-MS (gas chromatograph-mass spectrometer), with linalool identified as the main compound. Its cytotoxicity against MDA-MB-231, MCF-7 and HMLE cells was examined by MTT and cloning assays. Transwell and wound-healing assays were used to examine the inhibition of migration and invasion. Western blot, qRT-PCR and immunofluorescence staining were used to measure the mRNA and protein expression of factors related to EMT (snail, slug, E-cadherin, N-cadherin and vimentin). The essential oil of Erythrina corallodendron leaves was found to inhibit the proliferation, migration and invasion of breast cancer cells in a dose-dependent manner. The findings of this study suggest that the essential oil of E. corallodendron leaves may merit further investigation as a potential clinical or adjuvant drug for treating breast cancer migration and invasion.


Assuntos
Adenocarcinoma/tratamento farmacológico , Antineoplásicos Fitogênicos/análise , Neoplasias da Mama/tratamento farmacológico , Erythrina/química , Óleos Voláteis/uso terapêutico , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Humanos , Células MCF-7 , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/farmacologia , Fitoterapia , Folhas de Planta/química
4.
Int J Nanomedicine ; 14: 6439-6450, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31496696

RESUMO

Background and purpose: The essential oil derived from clove buds (Syzygium aromaticum) has been used as a chemopreventive agent in Ayurvedic medicine. The antiviral, antibacterial, and anticancer properties of its chemo-skeleton have motivated this study to explore its efficacy in pharmaceutics. Methods: Nanoscale-based emulsions were prepared by employing a spontaneous emulsification technique through self-assembly using varying concentrations of Tween 20 and Tween 80 surfactants. Their physicochemical properties and stability were studied in order to choose an optimum formulation which was clear and stable. The cytotoxicity of the stable oil-based emulsion system was evaluated using MTT assay, colony formation assay, and Annexin V-FITC assay against the thyroid cancer cell line (HTh-7). Results: All three methods verified apoptosis and reduction in cancer cell proliferation, making the formulation a promising candidate as an alternative cancer drug. The oil-based emulsion system was also tested for its antibacterial properties against Staphylococcus aureus. Membrane permeability studies proved its efficacy to permeate through cell membrane, thereby increasing the leakage of cytoplasmic contents. Conclusion: Many current treatments for cancers are aggressive yet ineffective. This study positions the clove bud-based nanoscale emulsion as a suitable candidate for further in vivo studies and trials as a cancer drug.


Assuntos
Antibacterianos/farmacologia , Antineoplásicos/farmacologia , Óleos Voláteis/farmacologia , Syzygium/química , Linhagem Celular Tumoral , Permeabilidade da Membrana Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Composição de Medicamentos , Emulsões , Humanos , Concentração de Íons de Hidrogênio , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Staphylococcus aureus/efeitos dos fármacos , Tensoativos/química , Viscosidade
5.
Bol. latinoam. Caribe plantas med. aromát ; 18(5): 480-491, sept. 2019. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1008273

RESUMO

In the present study, we investigated the antiproliferative activity of essential oil from leaves of Melissa officinalis L. grown in Southern Bosnia and Herzegovina. In vitro evaluation of antiproliferative activity of the M. officinalis essential oil was carried out on three human tumor cell lines: MCF-7, NCI-H460 and MOLT-4 by MTT assay. M. officinalis essential oil was characterized by high percentage of monoterpenes (77,5%), followed by the sesquiterpene fraction (14,5%) and aliphatic compounds (2,2%). The main constituents of the essential oil of M. officinalis are citral (47,2%), caryophyllene oxide (10,2%), citronellal (5,4%), geraniol (6,6%), geranyl acetate (4,1%) and ß- caryophyllene (3,8%). The essential oil showed significant antiproliferative activity against three cancer cell lines, MOLT-4, MCF-7, and NCI-H460 cells, with GI50 values of <5, 6±2 and 31±17 µg/mL, respectively. The results revealed that M. officinalis L. essential oil has a potential as anticancer therapeutic agent.


En el presente estudio, investigamos la actividad antiproliferativa del aceite esencial de las hojas de Melissa officinalis L. cultivadas en el sur de Bosnia y Herzegovina. La evaluación in vitro de la actividad antiproliferativa del aceite esencial de M. officinalis se llevó a cabo en tres líneas celulares de tumores humanos: MCF-7, NCI-H460 y MOLT-4 utilizando el ensayo de MTT. El aceite esencial de M. officinalis se caracterizó por un alto porcentaje de monoterpenos (77,5%), seguido de la fracción sesquiterpénica (14,5%) y compuestos alifáticos (2,2%). Los principales constituyentes del aceite esencial de M. officinalis fueron citral (47,2%), óxido de cariofileno (10,2%), citronelal (5,4%), geraniol (6,6%), acetato de geranilo (4, 1%), y ß-cariofileno (3,8%). El aceite esencial mostró una actividad antiproliferativa significativa contra las líneas celulares de cáncer MOLT-4, MCF-7 y NCI-H460, con valores GI50 de <5, 6±2 y 31±17 µg/mL, respectivamente. Los resultados revelaron que el aceite esencial de M. officinalis L. tiene potencial como agente terapéutico contra el cáncer.


Assuntos
Óleos Voláteis/farmacologia , Melissa , Antineoplásicos/farmacologia , Sesquiterpenos/análise , Técnicas In Vitro , Óleos Voláteis/química , Células Tumorais Cultivadas , Folhas de Planta , Monoterpenos/análise , Linhagem Celular Tumoral/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Antineoplásicos/química
6.
Int Braz J Urol ; 45(5): 1033-1042, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31408283

RESUMO

Cinnamomum cassia (Cinnamon) is a well-known traditional medicine with therapeutic benefits for centuries. We evaluated the effects of cinnamon essential oil (CEO) and its main component cinnamaldehyde (CA) on human corpus cavernosum (HCC) and rat CC. The essential oil of cinnamon was analyzed for the confirmation of the oil profile. HCC specimens from patients undergoing penile prosthesis surgery (age 48-69 years) were utilized for functional studies. In addition, erectile responses in anesthetized control and diabetic rats were evaluated in vivo after intracavernosal injection of CEO and CA, and rat CC strips were placed in organ baths. After precontraction with phenylephrine (10µM), relaxant responses to CEO and CA were investigated. CA (96.9%) was found as the major component. The maximum relaxation responses to CEO and CA were 96.4±3.5% and 96.0±5.0% in HCC and 97.5±5.5% and 96.8±4.8% in rat CC, respectively. There was no difference between control and diabetic rats in relaxation responses to CEO and CA. The relaxant responses obtained with essential oil and CA were not attenuated in the presence of nitric oxide synthase (NOS) inhibitor, and soluble guanylate cyclase inhibitor (sGS) in CC. In vivo, erectile responses in diabetic rats were lower than in control rats, which was restored after intracavernosal injection of CEO and CA. CEO and CA improved erectile function and relaxation of isolated strips of rat CC and HCC by a NO/cGMP-independent mechanism. Further investigations are warranted to fully elucidate the restorative effects of CEO and CA on diabetic erectile dysfunction.


Assuntos
Acroleína/análogos & derivados , Cinnamomum zeylanicum/química , Relaxamento Muscular/efeitos dos fármacos , Óleos Voláteis/farmacologia , Pênis/efeitos dos fármacos , Acroleína/farmacologia , Idoso , Análise de Variância , Animais , Disfunção Erétil/tratamento farmacológico , Disfunção Erétil/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Relaxamento Muscular/fisiologia , Ereção Peniana/efeitos dos fármacos , Ereção Peniana/fisiologia , Pênis/fisiopatologia , Fenilefrina/farmacologia , Inibidores da Fosfodiesterase 5/farmacologia , Ratos Sprague-Dawley , Reprodutibilidade dos Testes , Citrato de Sildenafila/farmacologia , Vasoconstritores/farmacologia
7.
Ann Parasitol ; 65(2): 129-138, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31376245

RESUMO

In order to finding of potent natural medication/agent which can kill giardial cysts in the interval between lysing of outer membrane of cysts due to exposure by acidic condition of stomach and their encystation in proximal small intestine, In-vitro antigiardial activity and GC-MS analysis of Eucalyptus globulus and Zingiber officinalis against Giardia lamblia were studied in simulated condition to human's body singly and in combination. Essential oils were extracted and their chemical components were identified via GC-MS method. After purification, cysts were exposure to acidic condition and were challenged by different concentrations of essential oils in simulated condition to human's body. Percentages of inactivated (killed) cysts as efficacy of antigiardial activity were calculated and analyzed statistically. Presence of 1,8-eucalyptol, α-pinene, α-terpineol acetate, etc. in essential oil of E. globulus and presence of geraniol, α-zingiberene, (E,E)-α-farnesene, etc. in Z. officinalis essential oil were identified. Highest antigiardial activity (73.55%) was observed for eucalyptus essential oil in time 480 minutes after exposure. Efficacies of ginger and combined essential oils were different in different times. This study shows considerable antigiardial activity for both of essential oils singly and in combination together against giardial cysts. In-vivo study of protective effect of these essential oils against giardiasis can be considered as a subject for next studies.


Assuntos
Eucalyptus , Cromatografia Gasosa-Espectrometria de Massas , Giardia lamblia , Gengibre , Óleos Voláteis , Eucalyptus/química , Giardia lamblia/efeitos dos fármacos , Gengibre/química , Humanos , Óleos Voláteis/farmacologia
8.
Rev Soc Bras Med Trop ; 52: e20180459, 2019 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-31365621

RESUMO

INTRODUCTION: The ability of Vitex trifolia and Vitex negundo essential oils to kill Aedes aegypti and Culex quinquefasciatus larvae was evaluated. METHODS: The larvae were treated with their respective essential oils at 50-125 ppm concentration. RESULTS: LC50 and LC90 for V. trifolia against Ae. aegypti and C. quinquefasciatus, and those for V. negundo against Ae. aegypti were 57.7+0.4, 77.9+0.9 ppm and 55.17+3.14, 78.28+2.23 ppm, and 50.86+0.9, 73.12+1.3 ppm, respectively. Eucalyptol and caryophyllene were the major components in Vitex trifolia and Vitex negundo essential oil, respectively. CONCLUSIONS: This study revealed potential larvicidal properties of essential oil from V. trifolia.


Assuntos
Aedes/efeitos dos fármacos , Inseticidas/farmacologia , Larva/efeitos dos fármacos , Mosquitos Vetores/efeitos dos fármacos , Óleos Voláteis/farmacologia , Vitex/química , Animais , Inseticidas/isolamento & purificação , Dose Letal Mediana , Óleos Voláteis/isolamento & purificação
9.
Chem Biodivers ; 16(10): e1900302, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31415713

RESUMO

The present study was designed to assess the influence of geographical factors on essential oil (EO) composition, along with antiradical potential and phytochemical contents of Ferulago angulata (Schltdl.) Boiss (Apiaceae) extracts for the first time. The aerial parts were hydrodistilled by Clevenger apparatus and subjected to gas chromatography coupled with flame ionization detector (GC/FID) and mass spectroscopy (GC/MS). The EO yields were significantly different from populations 'Mongar' (south-slope, 3000 m) with 1.34±0.06 % and 'Male-Amiri' (north slope, 2600 m) with 0.18±0.05 % of total oil. Thirty-nine compounds were identified from the EOs of nine populations. α-Pinene was the predominant component ranging from 20.84 to 49.06 % in 'Gandomkar' (north-slope, 2500 m) and 'Mongar' (3000 m), respectively. The methanolic extract of 'Mongar' (north-slope at 2500 m) possessed the highest total phenolic contents. Also, this population logically exhibited potent antiradical activity using both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) assays with EC50 of 42.07±4.12 µg/mL and 8.34±0.21 mmol Trolox® equivalents/g, respectively. Due to its moderate free-radical scavenging potential and high α-pinene content, the population 'Mongar' might be considered as a perspective raw material in food and phytopharmaceutical industries.


Assuntos
Antioxidantes/farmacologia , Apiaceae/química , Óleos Voláteis/farmacologia , Compostos Fitoquímicos/farmacologia , Antioxidantes/química , Compostos de Bifenilo/antagonistas & inibidores , Cromatografia Gasosa-Espectrometria de Massas , Irã (Geográfico) , Óleos Voláteis/química , Compostos Fitoquímicos/química , Picratos/antagonistas & inibidores , Espécies Reativas de Oxigênio/antagonistas & inibidores
10.
Chem Biodivers ; 16(10): e1900374, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31441194

RESUMO

The essential oils (EOs) obtained from the leaves of Iryanthera polyneura Ducke trees was chemically Assessed and tested for the ability of inhibiting the growth of Candida albicans, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans and S. sanguinis. The oil was also tested against breast (MCF-7) and prostate (PC-3) cancer cell lines. Minimum bactericidal concentrations (MBCs) and 50 % inhibition concentrations (IC50 ) values were obtained. EOs were active against Gram-positive bacteria. Spathulenol, α-cadinol and τ-muurolol were major components of EOs. The oils showed a higher cytotoxicity against PC-3 than MCF-7 cells, although the oils were active against both cell types. Oils obtained from leaves collected in the dry season were more active against E. faecalis, S. aureus and PC-3, while the oils obtained from leaves collected in the rainy season were more active against S. mutans, S. sanguinis and MCF-7. The antibacterial and cytotoxic activities of the essential oils from the leaves of I. polyneura are related to the seasonal climate variation and are influenced by compounds that are minor components of the oils.


Assuntos
Antibacterianos/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Myristicaceae/química , Óleos Voláteis/farmacologia , Folhas de Planta/química , Estações do Ano , Antibacterianos/síntese química , Antibacterianos/química , Antineoplásicos Fitogênicos/síntese química , Antineoplásicos Fitogênicos/química , Brasil , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Células MCF-7 , Testes de Sensibilidade Microbiana , Óleos Voláteis/síntese química , Óleos Voláteis/química , Células PC-3 , Floresta Úmida
11.
Chem Biodivers ; 16(10): e1900371, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31464066

RESUMO

The essential oils of five Lavandula stoechas cultivars grown in Thailand were characterized for their volatile compounds using GC-FID and GC/MS methods as well as screened for antibacterial and antioxidant activities. Dried aerial parts, including flowers and stems from each cultivar, were subjected to hydrodistillation for 4 h. The essential oil yields were 0.18 %-0.82 % w/w. Of the 95 compounds detected and identified, 1,8-cineole, fenchone, and camphor were considered the major compounds. Essential oil from each cultivar demonstrated different patterns of antibacterial activity and a variety of antioxidant properties. The highest antibacterial activity, MIC=0.39 mg mL-1 , was observed from the essential oil of L. stoechas 'major' (against Klebsiella pneumoniae and Salmonella typhimurium) and the essential oil of L. stoechas 'white lavender' (against S. typhimurium). The essential oil of L. stoechas×viridis 'St. Brelade' possessed the highest antioxidant capacity, as determined by the DPPH and ABTS assays (IC50 of 67.65 and 89.26 mg mL-1 , respectively). The results indicated that some of these essential oils could be used as key ingredients in lavender oil products in Thailand to increase their therapeutic efficacy, depending on their intended application.


Assuntos
Antibacterianos/farmacologia , Antioxidantes/farmacologia , Klebsiella pneumoniae/efeitos dos fármacos , Lavandula/química , Óleos Voláteis/farmacologia , Salmonella typhimurium/efeitos dos fármacos , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antioxidantes/química , Antioxidantes/isolamento & purificação , Benzotiazóis/antagonistas & inibidores , Compostos de Bifenilo/antagonistas & inibidores , Flores/química , Lavandula/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Picratos/antagonistas & inibidores , Componentes Aéreos da Planta/química , Ácidos Sulfônicos/antagonistas & inibidores , Tailândia
12.
Pestic Biochem Physiol ; 159: 59-67, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31400785

RESUMO

Phytophthora capsici is a plant oomycete pathogen, which causes many devastating diseases on a broad range of hosts. Zedoary turmeric oil (ZTO) is a kind of natural plant essential oil that has been widely used in pharmaceutical applications. However, the antifungal activity of ZTO against phytopathogens remains unknown. In this study, we found ZTO could inhibit P. capsici growth and development in vitro and in detached cucumber and Nicotiana benthamiana leaves. Besides, ZTO treatment resulted in severe damage to the cell membrane of P. capsici, leading to the leakage of intracellular contents. ZTO also induced a significant increase in relative conductivity, malondialdehyde concentration and glycerol content. Furthermore, we identified 50 volatile organic compounds from ZTO, and uncovered Curcumol, ß-elemene, curdione and curcumenol with strong inhibitory activities against mycelial growth of P. capsici. Overall, our results not only shed new light on the antifungal mechanism of ZTO, but also imply a promising alternative for the control of phytophthora blight caused by P. capsici.


Assuntos
Antifúngicos/farmacologia , Membrana Celular/efeitos dos fármacos , Óleos Voláteis/farmacologia , Phytophthora/efeitos dos fármacos , Extratos Vegetais/farmacologia , Óleos Vegetais/farmacologia , Curcuma , Phytophthora/metabolismo , Sesquiterpenos/farmacologia , Sesquiterpenos de Germacrano/farmacologia
13.
Environ Sci Pollut Res Int ; 26(25): 25414-25431, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31313235

RESUMO

Fungal and mycotoxin contamination of stored food items is of utmost concern throughout the world due to their hazardous effects on mammalian systems. Most of the synthetic chemicals used as preservatives have often been realised to be toxic to humans and also cause adverse environmental effects. In this respect, use of different plant products especially essential oils (EOs) and their bioactive compounds has been recognized as a green strategy and safer alternatives to grey synthetic chemicals in view of their long traditional use. The current nanoencapsulation technology has strengthened the prospective of EOs and their bioactive compounds in food preservation by enhancing their bioactivity and mitigating other problems regarding their large-scale application. Although, the antimicrobial potential of EOs and their bioactive compounds has been reviewed time to time by different food microbiologists, but very less is known about their mode of action. Based on these backgrounds, the present article provides an account on the antifungal and antimycotoxigenic mode of action of EOs as well as their bioactive compounds. In addition, the article also deals with the application of currently used nanoencapsulation approach to improve the stability and efficacy of EOs and their bioactive compounds against mycotoxigenic fungi causing deterioration of stored food items so as to recommend their large-scale application for safe preservation and enhancement of shelf life of food items during storage.


Assuntos
Antifúngicos/farmacologia , Contaminação de Alimentos/análise , Fungos/efeitos dos fármacos , Micotoxinas/análise , Óleos Voláteis/química , Antifúngicos/química , Microbiologia de Alimentos , Conservação de Alimentos/métodos , Humanos , Óleos Voláteis/farmacologia , Conservantes Farmacêuticos , Estudos Prospectivos
14.
Chem Biodivers ; 16(8): e1900204, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31298500

RESUMO

The purpose of this work was to determine the chemical composition and evaluate the antichemotactic, antioxidant, and antifungal activities of the essential oil obtained from the species Cryptocarya aschersoniana Mez, Cinnamomum amoenum (Ness & Mart.) Kosterm., and Schinus terebinthifolia Raddi, as well as the combination of C. aschersoniana essential oil and terbinafine against isolates of dermatophytes. Allo-aromadendrene, bicyclogermacrene, and germacrene B were identified as major compounds in essential oils. The essential oil of C. aschersoniana shown 100 % inhibitory effect on leukocyte migration at the concentration of 10 µg/mL while S. terebinthifolia oil presented 80.1 % inhibitory effect at the same concentration. Only S. terebinthifolia oil possessed free-radical-scavenging activity which indicates its antioxidant capacity. The essential oils were also tested against fungal isolates of dermatophyte species (Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis and Microsporum gypseum), resulting in MIC ranging from 125 µg/mL to over 500 µg/mL. C. aschersoniana oil combined with terbinafine resulted in an additive interaction effect. In this case, the essential oil may act as a complement to conventional therapy for the topical treatment of superficial fungal infections, mainly because it is associated with an anti-inflammatory effect.


Assuntos
Anacardiaceae/química , Antifúngicos/química , Cinnamomum/química , Cryptocarya/química , Óleos Voláteis/química , Anacardiaceae/metabolismo , Antifúngicos/farmacologia , Antioxidantes/química , Candida/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Cinnamomum/metabolismo , Cryptocarya/metabolismo , Testes de Sensibilidade Microbiana , Microsporum/efeitos dos fármacos , Neutrófilos/citologia , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Óleos Voláteis/farmacologia , Extratos Vegetais/química , Trichophyton/efeitos dos fármacos
15.
Chem Biodivers ; 16(8): e1900183, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31361076

RESUMO

This work describes the study of the chemical composition and bioactivity of the essential oils (EOs) of the different organs (leaves, flowers, stems and roots) from Eruca vesicaria. According to the GC and GC/MS analysis, all the EOs were dominated by erucin (4-methylthiobutyl isothiocyanate) with a percentage ranging from 17.9 % (leaves) to 98.5 % (roots). The isolated EOs were evaluated for their antioxidant (DPPH, ABTS and ß-carotene/linoleic acid), antibacterial and inhibitory property against α-amylase and α-glucosidase. Most EOs exhibited an interesting α-glucosidase and α-amylase inhibitory potential. The roots essential oil was found to be the most active with IC50 values of 0.80±0.06 and 0.11±0.01 µg mL-1 , respectively. The essential oil of roots exhibited the highest antioxidant activity (DPPH, PI=92.76±0.01 %; ABTS, PI=78.87±0.19; and ß-carotene, PI=56.1±0.01 %). The isolated oils were also tested for their antibacterial activity against two Gram-positive and three Gram-negative bacteria. Moderate results have been noted by comparison with Gentamicin used as positive control.


Assuntos
Antioxidantes/química , Brassicaceae/metabolismo , Óleos Voláteis/química , Antibacterianos/química , Antibacterianos/farmacologia , Brassicaceae/química , Flores/química , Flores/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Hipoglicemiantes/química , Óleos Voláteis/metabolismo , Óleos Voláteis/farmacologia , Folhas de Planta/química , Folhas de Planta/metabolismo , Raízes de Plantas/química , Raízes de Plantas/metabolismo , alfa-Glucosidases/química , alfa-Glucosidases/metabolismo
16.
Food Chem ; 299: 125109, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31295635

RESUMO

The aim of this research is to develop, characterize and utilize a multi-layer antibacterial film using chitosan (CS) and sodium alginate (SA) as biopolymers and cinnamon essential oil (CEO) as main antibacterial ingredients. The dense cross-section of SA layer in the scanning electron microscopy (SEM) analysis verified that layer-by-layer method improved physical and mechanical properties of CS-CEO single layer film. The thermogravimetric (TGA) and fourier transform infrared (FT-IR) analysis indicated that the layer-by-layer method changed the intermolecular interaction and the thermal stability. Importantly, the multi-layer film exhibited more sustained release and higher retention rate of CEO compared CS-CEO single layer film. The multi-layer coating showed a more significant and lasting inhibition of penicillium expansion which further demonstrated that the layer-by-layer method improved the release and retention of CEO in the multiphased system. To summarize, the multilayer film system is a promising controllable release system for loading essential oils.


Assuntos
Antifúngicos/farmacologia , Cinnamomum zeylanicum/química , Malus/microbiologia , Óleos Voláteis/farmacologia , Penicillium/efeitos dos fármacos , Alginatos/química , Antifúngicos/química , Quitosana/química , Preparações de Ação Retardada/química , Preparações de Ação Retardada/farmacologia , Frutas/efeitos dos fármacos , Frutas/microbiologia , Malus/efeitos dos fármacos , Microscopia Eletrônica de Varredura , Óleos Voláteis/farmacocinética , Penicillium/patogenicidade , Espectroscopia de Infravermelho com Transformada de Fourier , Eletricidade Estática , Termogravimetria
17.
J Sci Food Agric ; 99(14): 6552-6561, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31321781

RESUMO

BACKGROUND: Essential oils (EOs) are volatile natural compounds produced by plant secondary metabolism, and some of them exhibit antimicrobial activity. The objective of the present study was to determine the chemical composition the EOs of Eucalyptus staigeriana and Eucalyptus globulus, and their effect in vitro and in vivo against Botrytis cinerea and Colletotrichum acutatum, the most important fungal rot diseases of grapes. Moreover, grapes collected from field experiments were used to evaluate the impact of the alternative control on the alcoholic fermentation and wine composition. RESULTS: The major compound of E. staigeriana EO were citral 30.91% (19.74% geranial, 11.17% neral), 1.8-cineole (24.59%) and limonene (19.47%), while 1.8-cineole represented 68.26% of E. globulus EO. The two EOs showed in vitro antifungal activity against both pathogens. Eucalyptus staigeriana EO exhibited the highest activity inhibiting mycelial growth (MG) and conidial germination at 0.5 µL mL-1 . Moreover, this EO was able to reduce the incidence and severity of grey rot caused by B. cinerea and the severity of ripe rot caused by C. acutatum The alternative control did not significantly influence alcoholic fermentation, the physicochemical characteristics, and the volatile composition of wines. CONCLUSION: These results are promising and indicate that E. staigeriana EO might be further investigated as a natural alternative for the control of fungal rots on wine grapes. © 2019 Society of Chemical Industry.


Assuntos
Antifúngicos/farmacologia , Eucalyptus/química , Óleos Voláteis/farmacologia , Doenças das Plantas/prevenção & controle , Extratos Vegetais/farmacologia , Vitis/microbiologia , Antifúngicos/química , Botrytis/efeitos dos fármacos , Botrytis/crescimento & desenvolvimento , Colletotrichum/efeitos dos fármacos , Colletotrichum/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Micélio/efeitos dos fármacos , Micélio/crescimento & desenvolvimento , Óleos Voláteis/química , Doenças das Plantas/microbiologia , Extratos Vegetais/química
18.
Artigo em Inglês | MEDLINE | ID: mdl-31294650

RESUMO

The present study examines the influence of the natural preservatives carvacrol, eugenol, trans-cinnamaldehyde and the essential oil (EO) Origanum vulgare on ochratoxin A (OTA) production and the mycelial growth of two food-related moulds, Penicillium verrucosum and Aspergillus westerdijkiae, by broth macro-dilution assay for 21 days. With the addition of ½ minimum inhibitory concentration (MIC) carvacrol, eugenol and O. vulgare EO, the mycelial dry weight of both moulds decreased significantly over the whole incubation period of 7, 14 and 21 days. Trans-cinnamaldehyde slightly stimulated the growth of A. westerdijkiae and P. verrucosum at 14 and 21 days of incubation. Growth inhibition did not accompany inhibition of OTA production. Although the growth of both moulds was inhibited after the addition of ½ MIC carvacrol, eugenol and O. vulgare EO, the OTA production of the strong mycotoxin producer A. westerdijkiae was stimulated. Only trans-cinnamaldehyde inhibited the production of OTA with the addition of ½ MIC. P. verrucosum produced significantly less OTA than A. westerdijkiae, and its mycotoxin production was almost completely inhibited by the addition of ½ MIC of the natural preservatives.


Assuntos
Aspergillus/efeitos dos fármacos , Microbiologia de Alimentos , Conservantes de Alimentos/farmacologia , Micélio/efeitos dos fármacos , Ocratoxinas/biossíntese , Penicillium/efeitos dos fármacos , Acroleína/análogos & derivados , Acroleína/química , Acroleína/farmacologia , Aspergillus/química , Eugenol/química , Eugenol/farmacologia , Conservantes de Alimentos/química , Monoterpenos/química , Monoterpenos/farmacologia , Micélio/crescimento & desenvolvimento , Ocratoxinas/química , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Penicillium/química
19.
Int J Food Microbiol ; 306: 108266, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31319195

RESUMO

The antimicrobial activities of lemon oil based nanoemulsion and two different concentrations of lemon essential oil (100% and 10%) on food-brne pathogens (Staphylococcus aureus, Klebsiella pneumoniae, Enterococcus faecalis and Salmonella Paratyphi A) and fish spoilage bacteria (Photobacterium damselae, Enterococcus faecalis, Vibrio vulnificus, Proteus mirabilis, Serratia liquefaciens, and Pseudomonas luteola) were compared in terms of disc diffusion, minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC). The constitutes of extracted lemon essential oil were identified by using GC-MS. Viscosity, the mean droplet size, thermodynamic stability and refractive index of nanoemulsions were determined. The main components detected in the lemon essential oil were d-limonene, p-cymene, ß-pinene with percentages of 52.85%, 14.36%, and 13.69%, respectively. It was found that lemon nanoemulsion was more effective on food-borne pathogens except K. pneumoniae than 100% lemon essential oil. 10% lemon essential oil showed the highest inhibition effect on S. Paratyphi A. The conversion of the essential oil into nanoemulsion improved antimicrobial activity. According to value of MIC, both nanoemulsion and 100% essential oil inhibited bacterial growth of all of the pathogen bacteria tested whereas they were less effective on inhibition of fish spoilage bacteria. However, 10% essential oil was more effective on spoilage bacteria than pathogens. MBC showed that nanoemulsion and 100% lemon essential oil presented a noticeable bactericidal activity against S. paratyphi A whereas 10% lemon essential oil was found as ≥25 mg/mL against pathogens and spoilage bacteria. Therefore, the use of nanoemulsion based on lemon essential oil can have potential as a natural antimicrobial agent against food-borne pathogen and spoilage bacteria for fish processing industry.


Assuntos
Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Doenças Transmitidas por Alimentos/prevenção & controle , Óleos Voláteis/farmacologia , Óleos Vegetais/farmacologia , Citrus/classificação , Alimentos , Microbiologia de Alimentos , Doenças Transmitidas por Alimentos/microbiologia , Cromatografia Gasosa-Espectrometria de Massas , Testes de Sensibilidade Microbiana , Extratos Vegetais/farmacologia
20.
Rev Inst Med Trop Sao Paulo ; 61: e33, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31269109

RESUMO

Leishmaniasis is a disease that affects millions of people and it is an important public health problem. The drugs currently used for the treatment of leishmaniasis present undesirable side effects and low efficacy. In this study, we evaluated the in vitro activity of Melampodium divaricatum (MD-EO) and Casearia sylvestris (CS-EO) essential oils (EO) against promastigote and amastigote forms of Leishmania amazonensis. Sesquiterpenes E-caryophyllene (56.0%), germacrene D (12.7%) and bicyclogermacrene (9.2%) were identified as the main components of MD-EO, whereas E-caryophyllene (22.2%), germacrene D (19.6%) and bicyclogermacrene (12.2%) were the main constituents of CS-EO. CS-EO and E-caryophyllene were active against promastigote forms of L. amazonensis (IC50 24.2, 29.8 and 49.9 µg/mL, respectively). However, MD-EO, CS-EO and E-caryophyllene were more active against amastigote forms, with IC50 values of 10.7, 14.0, and 10.7 µg/mL, respectively. E-caryophyllene presented lower cytotoxicity against macrophages J774-A1 (CC50 of 62.1 µg/mL) than the EO. The EOs and E-caryophyllene should be further studied for the development of new antileishmanial drugs.


Assuntos
Antiprotozoários/farmacologia , Asteraceae/química , Casearia/química , Leishmania mexicana/efeitos dos fármacos , Óleos Voláteis/farmacologia , Antiprotozoários/isolamento & purificação , Humanos , Concentração Inibidora 50 , Leishmania mexicana/isolamento & purificação , Testes de Sensibilidade Parasitária
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA