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1.
Medicine (Baltimore) ; 98(36): e17009, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31490383

RESUMO

Erythrina corallodendron L., a kind of landscape tree, has long been used as a traditional medicine. In this study, the composition of essential oil extracted from the leaves was analysed by GC-MS (gas chromatograph-mass spectrometer), with linalool identified as the main compound. Its cytotoxicity against MDA-MB-231, MCF-7 and HMLE cells was examined by MTT and cloning assays. Transwell and wound-healing assays were used to examine the inhibition of migration and invasion. Western blot, qRT-PCR and immunofluorescence staining were used to measure the mRNA and protein expression of factors related to EMT (snail, slug, E-cadherin, N-cadherin and vimentin). The essential oil of Erythrina corallodendron leaves was found to inhibit the proliferation, migration and invasion of breast cancer cells in a dose-dependent manner. The findings of this study suggest that the essential oil of E. corallodendron leaves may merit further investigation as a potential clinical or adjuvant drug for treating breast cancer migration and invasion.


Assuntos
Adenocarcinoma/tratamento farmacológico , Antineoplásicos Fitogênicos/análise , Neoplasias da Mama/tratamento farmacológico , Erythrina/química , Óleos Voláteis/uso terapêutico , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Transição Epitelial-Mesenquimal/efeitos dos fármacos , Humanos , Células MCF-7 , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/farmacologia , Fitoterapia , Folhas de Planta/química
2.
Bol. latinoam. Caribe plantas med. aromát ; 18(5): 444-458, sept. 2019. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1008263

RESUMO

This review work focuses on how the secondary chemistry could help in the survival of plants in high mountain habitats under extreme environmental conditions. The elevated levels of stress in high areas of the tropic and subtropic change dramatically not only by following the annual cycles of winter and summer but they also change in a single day. Some species, however, are able to successfully grow at heights more than 3000 m in the tropical mountains due, in part, to highly specialized physiological processes that affect their physical and chemical responses. In this study, it describes some strategies of how the secondary metabolites could help the plants to stand the high levels of stress in the high mountain ecosystems.


Este trabajo de revisión se centra en cómo la química secundaria podría ayudar en la supervivencia de plantas en hábitats de alta montaña en condiciones ambientales extremas. Los altos niveles de estrés en las zonas altas del trópico y subtrópico cambian dramáticamente no solo al seguir los ciclos anuales de invierno y verano, sino que también cambian en un solo día. Sin embargo, algunas especies pueden crecer con éxito a alturas superiores a 3000 m en las montañas tropicales debido, en parte, a procesos fisiológicos altamente especializados que afectan sus respuestas físicas y químicas. En esta revisión, se describen algunas estrategias de cómo los metabolitos secundarios podrían ayudan a las plantas a soportar los altos niveles de estrés en los ecosistemas de alta montaña.


Assuntos
Plantas/química , Estresse Fisiológico , Óleos Voláteis/química , Altitude , Floroglucinol/análise , Floroglucinol/química , Terpenos/análise , Terpenos/química , Flavonoides/análise , Flavonoides/química
3.
Bol. latinoam. Caribe plantas med. aromát ; 18(5): 480-491, sept. 2019. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1008273

RESUMO

In the present study, we investigated the antiproliferative activity of essential oil from leaves of Melissa officinalis L. grown in Southern Bosnia and Herzegovina. In vitro evaluation of antiproliferative activity of the M. officinalis essential oil was carried out on three human tumor cell lines: MCF-7, NCI-H460 and MOLT-4 by MTT assay. M. officinalis essential oil was characterized by high percentage of monoterpenes (77,5%), followed by the sesquiterpene fraction (14,5%) and aliphatic compounds (2,2%). The main constituents of the essential oil of M. officinalis are citral (47,2%), caryophyllene oxide (10,2%), citronellal (5,4%), geraniol (6,6%), geranyl acetate (4,1%) and ß- caryophyllene (3,8%). The essential oil showed significant antiproliferative activity against three cancer cell lines, MOLT-4, MCF-7, and NCI-H460 cells, with GI50 values of <5, 6±2 and 31±17 µg/mL, respectively. The results revealed that M. officinalis L. essential oil has a potential as anticancer therapeutic agent.


En el presente estudio, investigamos la actividad antiproliferativa del aceite esencial de las hojas de Melissa officinalis L. cultivadas en el sur de Bosnia y Herzegovina. La evaluación in vitro de la actividad antiproliferativa del aceite esencial de M. officinalis se llevó a cabo en tres líneas celulares de tumores humanos: MCF-7, NCI-H460 y MOLT-4 utilizando el ensayo de MTT. El aceite esencial de M. officinalis se caracterizó por un alto porcentaje de monoterpenos (77,5%), seguido de la fracción sesquiterpénica (14,5%) y compuestos alifáticos (2,2%). Los principales constituyentes del aceite esencial de M. officinalis fueron citral (47,2%), óxido de cariofileno (10,2%), citronelal (5,4%), geraniol (6,6%), acetato de geranilo (4, 1%), y ß-cariofileno (3,8%). El aceite esencial mostró una actividad antiproliferativa significativa contra las líneas celulares de cáncer MOLT-4, MCF-7 y NCI-H460, con valores GI50 de <5, 6±2 y 31±17 µg/mL, respectivamente. Los resultados revelaron que el aceite esencial de M. officinalis L. tiene potencial como agente terapéutico contra el cáncer.


Assuntos
Óleos Voláteis/farmacologia , Melissa , Antineoplásicos/farmacologia , Sesquiterpenos/análise , Técnicas In Vitro , Óleos Voláteis/química , Células Tumorais Cultivadas , Folhas de Planta , Monoterpenos/análise , Linhagem Celular Tumoral/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Antineoplásicos/química
4.
Bol. latinoam. Caribe plantas med. aromát ; 18(5): 518-526, sept. 2019. tab, ilus
Artigo em Inglês | LILACS | ID: biblio-1008290

RESUMO

Valeriana amurensis Smir. ex Kom. widely distributed in the northeast region of China and some region in Russia and Korea, and its underground parts (roots and rhizomes) being used to cure nervous system diseases such as insomnia. The active components including the essential oil and iridoids of underground parts were investigated in different harvest periods in order to evaluate the quality for the roots and rhizomes of V. amurensis. The content of the essential oil was obtained by hydrodistillation and bornyl acetate in the oil was quantitated by GC-EI. The iridoids, valepotriates were determined by potentiometric titration and the main component, valtrate was quantitated by HPLC-UV. The factors of biomass were considered in the determination of collection period. Statistical analysis of results showed that, the highest content of the essential oil per plant was 22.69 µl in withering period and then 21.58 µl in fruit ripening period, while the highest contents of bornyl acetate, valepotriates and valtrate per plant were 2.82 mg, 31.90 mg and 0.98 mg in fruit ripening period separately. Fruit ripening period was decided as the best harvest period for the content of active constituents and output of drug, and it would provide scientific basis for the artificial cultivation of V. amurensis.


Valeriana amurensis Smir. ex Kom. Se distribuye ampliamente en la región noreste de China y en algunas regiones de Rusia y Corea, y sus partes subterráneas (raíces y rizomas) se utilizan para curar enfermedades del sistema nervioso como el insomnio. Se investigaron los componentes activos, incluidos el aceite esencial y los iridoides de las partes subterráneas de V. amurensis en diferentes períodos de cosecha para evaluar la calidad de las raíces y rizomas. El contenido del aceite esencial se obtuvo mediante hidrodestilación y el acetato de bornilo en el aceite se cuantificó por GC-EI. Los iridoides, valepotriatos se determinaron mediante valoración potenciométrica y el componente principal, el valtrato se cuantificó por HPLC-UV. Los factores de biomasa fueron considerados en la determinación del período de recolección. El análisis estadístico de los resultados mostró que el mayor contenido de aceite esencial por planta fue de 22,69 µl en el período de marchitación y luego de 21,58 µl en el período de maduración de la fruta, mientras que el mayor contenido de acetato de bornilo, valepotriatos y valtrato por planta fue de 2.82 mg, 31.90 mg y 0,98 mg, respectivamente, en el período de maduración de la fruta por separado. Se definió el período de maduración de la fruta como el mejor período de cosecha para el contenido de constituyentes activos y la producción de droga, lo cual proporcionaría una base científica para el cultivo artificial de V. amurensis.


Assuntos
Valeriana/química , Óleos Voláteis/química , Raízes de Plantas/química , Estações do Ano , Bornanos/análise , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas por Ionização por Electrospray , Rizoma/química , Iridoides/análise
5.
Pestic Biochem Physiol ; 158: 101-111, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31378344

RESUMO

Standard chemical insecticides present mainly neurotoxic effects and are becoming less and less effective due to insects developing resistance to them. One of the innovative strategies to control insects pests is to find a way to increase the sensitivity of the target sites in the insect nervous system to the applied insecticides. In the presented research, we proposed menthol, a component of essential oils, as a factor increasing the effectiveness of bendiocarb, a carbamate insecticide. The aim of our study was to evaluate the potentiation of the bendiocarb effect by menthol. In toxicity tests performed on Periplaneta americana, menthol (0.1 µM) accelerated the lethal effect of bendiocarb, primarily in its low concentrations (lower than 0.05 mM). In the presence of menthol (1 and 0.1 µM), the ability of insects to turn back from its dorsal to the normal ventral side was significantly lower than with bendiocarb (1 µM) alone. We also evaluated the effectiveness of chemicals on the activity of the ventral nerve cord of the cockroach. In this preparation, bendiocarb (1 µM and higher concentrations) caused an irregular, spontaneous bursts of action potentials. The total nerve activity (including the response to stimulation and spontaneous firing) was much higher when bendiocarb was applied in the presence of menthol (1 µM). The effect of menthol was similar to the octopamine effect and was abolished by phentolamine, the octopamine receptor antagonist. Our results clearly indicated a strengthening effect of menthol on bendiocarb effectiveness; potentiation occurred through octopamine receptors activation.


Assuntos
Carbamatos/farmacologia , Baratas/efeitos dos fármacos , Inseticidas/farmacologia , Mentol/farmacologia , Óleos Voláteis/química , Fenilcarbamatos/farmacologia , Acetilcolinesterase/metabolismo , Animais , Interações de Medicamentos , Resistência a Inseticidas
6.
Food Chem ; 298: 125079, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260959

RESUMO

Citral-in-water emulsions were prepared with two different essential oil concentrations of 2.5 and 5.0% (w/w), then spray-dried in the presence of the same amount of maltodextrins (20%). The microcapsules were prepared with two different emulsifier compositions: monolayer microcapsules (ML) stabilized by sodium caseinate alone and layer-by-layer microcapsules (LBL) stabilized by sodium caseinate and pectin. The encapsulation efficiency was higher for LBL microcapsules (e.g. 99.6 ±â€¯0.4% for 2.5% citral) than that for ML ones (e.g. 78.6 ±â€¯0.6% for 2.5% citral) which confirm that the additional pectin layer was able to protect citral during the spray-drying process whatever citral concentration. Furthermore, our results showed that the antibacterial activity of the obtained microcapsules significantly depends on both citral concentration and interfacial membrane composition. The presence of two layers surrounding the citral droplets may result in a progressive and controlled release of the encapsulated citral.


Assuntos
Emulsões/química , Monoterpenos/química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Cápsulas/química , Caseínas/química , Dessecação , Listeria/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Óleos Voláteis/química , Tamanho da Partícula , Pectinas/química , Polissacarídeos/química , Staphylococcus aureus/efeitos dos fármacos
7.
Chem Biodivers ; 16(8): e1900204, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31298500

RESUMO

The purpose of this work was to determine the chemical composition and evaluate the antichemotactic, antioxidant, and antifungal activities of the essential oil obtained from the species Cryptocarya aschersoniana Mez, Cinnamomum amoenum (Ness & Mart.) Kosterm., and Schinus terebinthifolia Raddi, as well as the combination of C. aschersoniana essential oil and terbinafine against isolates of dermatophytes. Allo-aromadendrene, bicyclogermacrene, and germacrene B were identified as major compounds in essential oils. The essential oil of C. aschersoniana shown 100 % inhibitory effect on leukocyte migration at the concentration of 10 µg/mL while S. terebinthifolia oil presented 80.1 % inhibitory effect at the same concentration. Only S. terebinthifolia oil possessed free-radical-scavenging activity which indicates its antioxidant capacity. The essential oils were also tested against fungal isolates of dermatophyte species (Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis and Microsporum gypseum), resulting in MIC ranging from 125 µg/mL to over 500 µg/mL. C. aschersoniana oil combined with terbinafine resulted in an additive interaction effect. In this case, the essential oil may act as a complement to conventional therapy for the topical treatment of superficial fungal infections, mainly because it is associated with an anti-inflammatory effect.


Assuntos
Anacardiaceae/química , Antifúngicos/química , Cinnamomum/química , Cryptocarya/química , Óleos Voláteis/química , Anacardiaceae/metabolismo , Antifúngicos/farmacologia , Antioxidantes/química , Candida/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Cinnamomum/metabolismo , Cryptocarya/metabolismo , Testes de Sensibilidade Microbiana , Microsporum/efeitos dos fármacos , Neutrófilos/citologia , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Óleos Voláteis/farmacologia , Extratos Vegetais/química , Trichophyton/efeitos dos fármacos
8.
Chem Biodivers ; 16(8): e1900183, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31361076

RESUMO

This work describes the study of the chemical composition and bioactivity of the essential oils (EOs) of the different organs (leaves, flowers, stems and roots) from Eruca vesicaria. According to the GC and GC/MS analysis, all the EOs were dominated by erucin (4-methylthiobutyl isothiocyanate) with a percentage ranging from 17.9 % (leaves) to 98.5 % (roots). The isolated EOs were evaluated for their antioxidant (DPPH, ABTS and ß-carotene/linoleic acid), antibacterial and inhibitory property against α-amylase and α-glucosidase. Most EOs exhibited an interesting α-glucosidase and α-amylase inhibitory potential. The roots essential oil was found to be the most active with IC50 values of 0.80±0.06 and 0.11±0.01 µg mL-1 , respectively. The essential oil of roots exhibited the highest antioxidant activity (DPPH, PI=92.76±0.01 %; ABTS, PI=78.87±0.19; and ß-carotene, PI=56.1±0.01 %). The isolated oils were also tested for their antibacterial activity against two Gram-positive and three Gram-negative bacteria. Moderate results have been noted by comparison with Gentamicin used as positive control.


Assuntos
Antioxidantes/química , Brassicaceae/metabolismo , Óleos Voláteis/química , Antibacterianos/química , Antibacterianos/farmacologia , Brassicaceae/química , Flores/química , Flores/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Hipoglicemiantes/química , Óleos Voláteis/metabolismo , Óleos Voláteis/farmacologia , Folhas de Planta/química , Folhas de Planta/metabolismo , Raízes de Plantas/química , Raízes de Plantas/metabolismo , alfa-Glucosidases/química , alfa-Glucosidases/metabolismo
9.
Cryo Letters ; 40(4): 219-225, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31278402

RESUMO

BACKGROUND: Rosmarinus officinalis essential oil (Rom) has been reported recently to be of interest for use in sperm cryopreservation. However, related to its lipophilic characteristics, encapsulation in cyclodextrin could enhance Rom positive effects by increasing its solubility in sperm extenders. OBJECTIVE: To compare the effect of Rom preloaded in hydroxypropyl-ß-cyclodextrin (Rom-cd) to Rom alone (Rom) on ram sperm conserved at 4°C. MATERIALS AND METHODS: Ram epididymal sperm was collected from six testes. Each collected sperm was split into four equal aliquots. The control aliquot was diluted with Tris extender (Tris + citric acid + fructose + penicillin), two aliquots were treated with Rom at 0.5µl ml-1 and 1µl ml-1 respectively, and one aliquot treated with Rom-cd at 1µl ml-1. All sperm aliquots were analyzed for motility after 0, 2, 4, 24 and 48 h of storage at 4°C using a Computer Aided Semen Analysis (CASA). Membrane integrity and oxidative stress status were measured after 48 h of storage. RESULTS: The results indicated that motility parameters were best preserved in the extender containing Rom-cd compared to the groups treated by Rom without cyclodextrin. Rom alone resulted to higher sperm motility than the control group. Lower oxidative stress and more cell membrane protection were observed in Rom treated samples, especially when using Rom-cd. CONCLUSION: The ability of Rom to protect ram sperm against cryopreservation damages was improved after encapsulation in hydroxypropyl-ß-cyclodextrin (HPßCD).


Assuntos
Óleos Voláteis/química , Rosmarinus/química , Preservação do Sêmen/veterinária , Motilidade Espermática , Espermatozoides/efeitos dos fármacos , beta-Ciclodextrinas/química , Animais , Membrana Celular , Criopreservação/veterinária , Masculino , Estresse Oxidativo , Ovinos , Espermatozoides/citologia
10.
Bol. latinoam. Caribe plantas med. aromát ; 18(4): 435-443, jul. 2019. ilus, tab
Artigo em Inglês | LILACS | ID: biblio-1008258

RESUMO

Volatiles compounds are involved in defensive induction against insects, playing an important role in insect-plant interaction being induced by response to mechanical damage. However, they could decrease according to the domestication degree in cultivated plants. Currently, it has been established that secondary metabolites are reduced due to the domestication process in murtilla. Hence, the follow question emerges: Are volatile organic compounds induced by mechanical damage reduced in cultivated murtilla plants in relation to wild plants? Two cultivated ecotypes and their respective wild counterparts were sampled. Volatiles compounds were obtained using Porapak-Q columns and analyzed by gas chromatography. Results showed that compounds as 2-hexanone, α-pinene, 2-thujene, 3-thujene and 1,8- cineole were more abundant in wild plants exposed to a mechanical damage than cultivated plants. Hence, these compounds have been associated to induced defense, these results suggest that domestication reduced the induction of defensive volatiles in cultivated murtilla in response to mechanical damage.


Los compuestos volátiles están implicados en la defensa inducida contra insectos, desempeñando un papel importante en esta interacción. Sin embargo, estos compuestos podrían disminuir según el grado de domesticación. Actualmente, se ha reportado que algunos metabolitos secundarios son reducidos en plantas de murtilla domesticadas. Por lo tanto, surge la siguiente pregunta de investigación: ¿Los compuestos orgánicos volátiles inducidos por el daño mecánico son reducidos en plantas cultivadas de murtilla en comparación con plantas silvestres? Para dos ecotipos cultivados y sus respectivas contrapartes silvestres, los compuestos volátiles fueron capturados usando columnas de Porapak-Q y las muestras analizadas por cromatografía gaseosa. Los resultados mostraron que compuestos tales como 2- hexanona, α-pineno, 2-tujeno, 3-tujeno y 1,8-cineol fueron más abundantes en plantas silvestres expuestas a daño mecánico que en cultivadas. Debido a que estos compuestos se han asociado a defensa inducida, estos resultados sugieren que la domesticación reduce la inducción de volátiles en plantas cultivadas sometidas a daño mecánico.


Assuntos
Animais , Óleos Voláteis/metabolismo , Myrtaceae/metabolismo , Myrtaceae/microbiologia , Domesticação , Insetos/fisiologia , Sesquiterpenos/análise , Sesquiterpenos/metabolismo , Óleos Voláteis/química , Myrtaceae/química , Monoterpenos/análise , Monoterpenos/metabolismo , Meio Selvagem , Larva/fisiologia , Cromatografia Gasosa-Espectrometria de Massas
11.
Environ Sci Pollut Res Int ; 26(22): 23198-23205, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31201707

RESUMO

The objective of this work was to analyze four essential oils (EOs) from Rhododendron species in China and evaluate their repellent activity against Tribolium castaneum, Lasioderma serricorne, and Liposcelis bostrychophila adults. These four EOs from Rhododendron species, including R. capitatum, R. przewalskii, R. mucronulatum, and R. micranthum, were obtained by hydrodistillation. Major components of four EOs were identified as sesquiterpenoids by GC-MS. The relatively high components included cedrene (22.20%), borneol (36.64%), 4-(2,3,4,6-tetramethylphenyl)-3-buten-2-one (27.74%), and germacrene D (27.60%). Repellent activity of EOs from Rhododendron species was investigated against T. castaneum, L. serricorne, and L. bostrychophila adults for the first time. In this study, EOs had demonstrated their repellent activities against three stored-product insects in 2- and 4-h exposure. The above results can not only provide comprehensive utilization of plant resources of Rhododendron genus but also establish a very good perspective of novel application to control stored-product insects.


Assuntos
Besouros/efeitos dos fármacos , Repelentes de Insetos/química , Óleos Voláteis/química , Animais , China , Cromatografia Gasosa-Espectrometria de Massas , Rhododendron , Sesquiterpenos/química , Tribolium/química
12.
BMC Complement Altern Med ; 19(1): 146, 2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31227024

RESUMO

BACKGROUND: There is increasing interest in the pharmaceutical and food industries to substitute synthetic chemicals with naturally occurring compounds possessing bioactive properties. Plants are valuable sources of bioactive compounds. The present study investigates the chemical composition and antioxidant, antimicrobial, and anticancer activities of ethanolic extracts (EEs) and essential oils (EOs) from two species in the Lamiaceae family, Ocimum basilicum L. and Thymus algeriensis Boiss. & Reut., cultivated in the Algerian Saharan Atlas. METHODS: The total flavonoid contents of the plants' ethanolic extracts were determined by the aluminium chloride method, while the total phenols were determined using the Folin-Ciocalteu method. Essential oils were obtained by hydrodistillation of the aerial parts of the plants and were analysed by GC-MS. The free radical-scavenging ability and antioxidant potential of the plants' EEs and EOs were probed using the 2, 2-diphenyl-picrylhydrazyl radical-scavenging, ABTS radical-scavenging, ferric-reducing power and phosphomolybdenum assays. The antimicrobial activities were evaluated against several pathogens characteristic of gram-negative bacteria (three species), gram-positive bacteria (three species) and fungi (two species). The microdilution method was used to estimate the minimum inhibitory concentrations (MICs). The oils' anticancer potential against several cancer types was also studied using the MTT assay and reported as the toxic doses that resulted in a 50% reduction in cancer cell growth (LD50). RESULTS: Phenolic compounds in the EEs from both plants were analysed by HPLC and demonstrated a rich flavonoid content. Chemical analysis of the essential oil from Ocimum basilicum revealed 26 unique compounds, with linalool (52.1%) and linalyl acetate (19.1%) as the major compounds. A total of 29 compounds were identified in the essential oil from Thymus algeriensis, with α-terpinyl acetate (47.4%), neryl acetate (9.6%), and α-pinene (6.8%) as the major compounds. The ethanolic extracts and essential oils from both plants exhibited moderate antioxidant activities and moderate to weak antimicrobial activities. Furthermore, anticancer activities against the examined human cancer cell lines were associated with only the EOs from both plants, with LD50 values ranging between 300 and 1000 µg/mL. CONCLUSION: The results suggest that the bioactive compounds found in the ethanolic extracts and essential oils from Ocimum basilicum and Thymus algeriensis, with diverse antioxidant, antimicrobial and anticancer activities, may have beneficial applications in nutraceutical and pharmaceutical technologies.


Assuntos
Ocimum basilicum/química , Óleos Voláteis/química , Óleos Vegetais/química , Thymus (Planta)/química , Argélia , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Linhagem Celular Tumoral , Sobrevivência Celular , Cromatografia Líquida de Alta Pressão , Fungos/efeitos dos fármacos , Fungos/crescimento & desenvolvimento , Humanos , Fenol/química , Fenol/farmacologia , Óleos Vegetais/farmacologia
13.
Plant Physiol Biochem ; 141: 142-153, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31163341

RESUMO

Plant growth promoting bacteria (PGPB) are agriculturally important soil bacteria that increase plant growth. We subjected peppermint to inoculation with three species of PGPB. After inoculation, the plants were sprayed with methyl jasmonate solution (MeJA) or SA (salicylic acid). Then, the plants were harvested and the plant growth parameters, trichome density, EO content and endogenous phytohormones were measured. Shoot fresh weight was reduced in plants inoculated and treated with MeJA whereas EO content varied depending on the MeJA concentration applied. Plants inoculated and treated with MeJA 2 mM showed the maximum increase in EO production, revealing a synergism between PGPB and MeJA. SA treatments also enhanced EO yield. The increased growth and EO production observed upon PGPB application were at least partly due to an increase in the JA and SA concentrations in the plant, as well as to an associated rise in the glandular trichome density.


Assuntos
Acetatos/farmacologia , Ciclopentanos/química , Ciclopentanos/farmacologia , Mentha piperita/química , Óleos Voláteis/química , Oxilipinas/química , Oxilipinas/farmacologia , Ácido Salicílico/química , Tricomas/química , Bacillus subtilis , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Mentha piperita/microbiologia , Óleos Voláteis/isolamento & purificação , Desenvolvimento Vegetal , Reguladores de Crescimento de Planta/química , Folhas de Planta/química , Brotos de Planta/química , Pseudomonas fluorescens , Pseudomonas putida
14.
Chem Biodivers ; 16(8): e1900131, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31173470

RESUMO

Most species of the genus Laggera are often used in traditional and folk medicines for the treatment of jaundice, inflammation, leukemia, removing phlegm, bronchitis and bacterial diseases. The essential oils obtained from Laggera plants are rich sources of oxygenated monoterpenes and sesquiterpenes. Among oxygenated monoterpenes, aromatic ether 2,5-dimethoxy-p-cymene is the most abundant and dominant compound of many essential oils of the Laggera species. Till today, to the best of our knowledge, chemical compounds of the essential oils and/or extracts of only eight Laggera species were reported from different countries. Thus, this review presents the chemical compositions and biological activities of the essential oils of these plants studied in thirteen countries. In addition, it discusses the reported ethnobotanical and ethnopharmacological information as well as biological activities of the extracts and some of the isolated compounds of Laggera plants species.


Assuntos
Asteraceae/metabolismo , Óleos Voláteis/química , Antioxidantes/química , Candida/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Medicina Tradicional , Óleos Voláteis/farmacologia , Fenóis/química , Extratos Vegetais/química , Compostos Orgânicos Voláteis/química
15.
Chem Biodivers ; 16(8): e1900278, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31207097

RESUMO

Using synthetic chemicals in industry and agriculture has led to several environmental problems. Thus, plant products derived from volatile oils (VOs) could be a potential green source for bioherbicides. Little is known about the VOs of Lactuca serriola. Hence, the present study aimed to characterize the VOs chemical composition from the aerial parts of L. serriola, assessment of antioxidant activity, and evaluate allelopathic potential against the noxious weed Bidens pilosa. The VOs were extracted by hydrodistillation and analyzed by GC/MS. The VOs from the Egyptian ecospecies of L. serriola were found to comprise 34 compounds mainly oxygenated sesquiterpenes and diterpenes. The major compounds were isoshyobunone (64.22 %), isocembrol (17.35 %), and alloaromadendrene oxide-1 (7.32 %). So, L. serriola can be considered as a good source for isoshyobunone, considering that it has a much higher concentration than any other plants. Also, this plant has a high content of the oxygenated diterpene compound, isocembrol, which is rarely found in the VOs of most plants. The VOs expressed strong antioxidant activity. Also, for the first time, our results showed a strong allelopathic activity of VOs from L. serriola on germination and seedling growth of the noxious weed, B. pilosa. We suppose that the activity of the VOs from L. serriola could be attributed to these previously mentioned major compounds, as they represent about 89 % of the total identified oil constituents. Nevertheless, to evaluate these compounds as new allelochemicals, further study is needed to test the allelopathic activity of authenticated standard of these compounds either singular or in combination on several weeds as well as evaluate the safety, and improve the efficacy and stability at the field scale.


Assuntos
Antioxidantes/química , Asteraceae/química , Diterpenos/química , Óleos Voláteis/química , Sesquiterpenos/química , Asteraceae/metabolismo , Bidens/crescimento & desenvolvimento , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Germinação/efeitos dos fármacos , Óleos Voláteis/farmacologia , Componentes Aéreos da Planta/química , Componentes Aéreos da Planta/metabolismo , Extratos Vegetais/química , Sesquiterpenos/isolamento & purificação , Sesquiterpenos/farmacologia
16.
Food Chem ; 295: 16-25, 2019 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-31174745

RESUMO

The instability of Laurel essential oil (LEO) and easy leakage of silver nanoparticle (AgNP) restricts their application in food field. To control their release, liposomes were used to encapsulate LEO and AgNPs (Lip-LEO-AgNPs), and mixed with chitosan to coat polyethylene (PE) films (PC-Lip/LEO/AgNPs) to package pork. The results show that only about 29.30% of LEO and 11.79% of AgNPs were released from the liposome after 7 days at 25 °C. Nevertheless, Lip-LEO-AgNPs showed good antioxidant properties. Moreover, PC-Lip/LEO/AgNPs films had good antimicrobial activity. Evaluation tests on pork indicate that PC-Lip/LEO/AgNPs films could keep the quality of pork at 4 °C for 15 days while pure PE film only kept it for 9 days, and that the coating films had no cytotoxicity. PC-Lip/LEO/AgNPs films exhibited significant combined action in functional packaging to extend storage period, and provided a new idea for the application of LEO and AgNPs in food.


Assuntos
Conservação de Alimentos/métodos , Lipossomos/química , Óleos Voláteis/química , Carne Vermelha , Sesquiterpenos/química , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Quitosana/química , Qualidade dos Alimentos , Armazenamento de Alimentos/métodos , Humanos , Lipossomos/farmacologia , Nanopartículas Metálicas/química , Óleos Voláteis/farmacologia , Tamanho da Partícula , Sesquiterpenos/farmacologia , Prata/química , Suínos , Temperatura Ambiente
17.
Chem Biodivers ; 16(7): e1900125, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31241852

RESUMO

The essential oil composition of the aerial parts of Artemisia magellanica Sch. Bip. (Asteraceae), native to Patagonia, was analyzed by GC-FID-MS. This is the first report on the essential oil composition of A. magellanica. A total of 113 components were identified accounting for 95.6-95.7 % of the oil. The essential oil was characterized by a high percentage of γ-costol (21.0-43.5 %), selina-4,11-diene, (Z)-ß-ocimene, (E)-ß-farnesene, (Z)-en-yn-dicycloether and 23 different esters (28.7 %). In turn, Artemisia biennis, a species native to North America, which is considered by some authors to be conspecific with A. magellanica, yielded an essential oil that was rich in (Z)-ß-ocimene (34.7 %), (E)-ß-farnesene (40.0 %) and the acetylenes (Z)- and (E)-en-yn-dicycloethers (11.0 %). Thus, as A. biennis lacks the three main components present in A. magellanica, namely γ-costol, 2-methylbutyl 2-methylbutyrate and selina-4,11-diene, these compounds could be considered as potential chemical markers for A. magellanica since they are absent or only found as minor constituents in other members of the genus. The data presented herein is also useful for genus taxonomy.


Assuntos
Artemisia/química , Óleos Voláteis/isolamento & purificação , Estrutura Molecular , Óleos Voláteis/química , Especificidade da Espécie
18.
J Photochem Photobiol B ; 197: 111538, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31247385

RESUMO

The effects of topically administered snake (Deinagkistrodon acutus) oil and its main fatty acid components on skin photodamage were explored. Epidermal thickness, mice body weight, antioxidant enzymes (superoxide dismutase, glutathione peroxidase, catalase), inflammatory cytokines (tumor necrosis factor alpha and interleukin-6), skin histology, collagen content, and metalloproteinase-1 indicators were analyzed. The results show that topical application of both snake oil and its main fatty acids recovered ultraviolet B (UVB) irradiation induced antioxidant enzymes depletion, prevented metalloproteinase-1. Snake oil and its main fatty acids suppressed dermal infiltration of inflammatory cells and reduced inflammatory cytokines levels. Notably, there was no significant difference in the antioxidant activity but a significant difference in the anti-inflammatory activity between fatty acids and snake oil under the same dose. Finally, snake oil and its main fatty acids inhibited UVB-induced histological damage such as epidermal thickening, collagen fiber and elastic fiber destruction. Our study demonstrated for the first time in KM mice that snake oil exhibited prominent photoprotection activity by protecting the activity of antioxidant enzymes and inhibiting inflammatory factors, as well as reducing the generation of MMP-1. What's more, the main fatty acids in snake oil play an important role in preventing photo-damage especially in protecting antioxidant enzyme activity.


Assuntos
Anti-Inflamatórios/farmacologia , Ácidos Graxos/farmacologia , Óleos Voláteis/química , Pele/efeitos dos fármacos , Serpentes/metabolismo , Raios Ultravioleta , Animais , Anti-Inflamatórios/química , Antioxidantes/metabolismo , Catalase/metabolismo , Citocinas/metabolismo , Epiderme/fisiologia , Ácidos Graxos/química , Feminino , Cromatografia Gasosa-Espectrometria de Massas , Glutationa Peroxidase/metabolismo , Hidroxiprolina/análise , Interleucina-6/genética , Interleucina-6/metabolismo , Metaloproteinase 1 da Matriz/metabolismo , Camundongos , Pele/metabolismo , Pele/patologia , Pele/efeitos da radiação , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismo
19.
Chemotherapy ; 64(1): 1-7, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31234166

RESUMO

Infections with herpes simplex virus type (HSV)-1 and HSV-2 are distributed worldwide. Although standard therapies with acyclovir and other synthetic drugs are available, the safety and efficacy of these drugs are limited due to the development of drug resistance and adverse side effects. The literature on essential oils and isolated compounds was reviewed regarding their antiviral activities against HSV-1 and HSV-2. The present overview aims to review experimental data and clinical trials focusing on the antiviral activity of selected essential oils and isolated oil components. HSV was found to be susceptible to many essential oils and their constituents. Whereas some essential oils and compounds exhibit direct virucidal activity or inhibit intracellular replication, many essential oils and compounds interact with HSV particles thereby inhibiting cell adsorption. Ayclovir-resistant HSV strains are also susceptible to essential oils since their mode of action is different from the synthetic drug. There are numerous publications on the antiherpetic activity of essential oils and their isolated active compounds. This field of research is still growing, and more clinical trials are required to explore the full potential of different essential oils for the topical treatment of herpetic infections.


Assuntos
Herpes Simples/tratamento farmacológico , Óleos Voláteis/uso terapêutico , Antivirais/química , Antivirais/farmacologia , Antivirais/uso terapêutico , Adesão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Ensaios Clínicos como Assunto , Humanos , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Simplexvirus/efeitos dos fármacos , Simplexvirus/fisiologia
20.
Food Chem Toxicol ; 131: 110559, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31176927

RESUMO

The family Piperaceae is known for presenting in its species flavoring, healing and antimicrobial properties among others. The objective of the present study was: to study the chemical profile of the essential oil of Piper rivinoides (EOPR); to analyze its anti-bacterial and antifungal potential, as well as to evaluate the antifungal and antibiotic-modifying capacity. The chemical constituents were identified by gas chromatography with flame ionization detector (GC-FID), allowing the identification of 7 constituents of a total of 86.99%. E-Isoelemicin was identified as the main constituent of petroleum (40.81%). Clinically relevant MIC results were obtained against fungi in which the inhibitory concentration remained <256 µg/mL, as for Candida albicans 4127 (217.6 µg/mL). The association of EOPR with an antifungal showed a high synergistic affinity against the strains of C. tropicalis 40042 and 4262. We concluded that no intrinsic EOPR activity was observed at any concentrations tested against bacteria. However, EOPR associated with Gentamicin acted synergistically against S. aureus 10 and Escherichia coli 06, but with Erythromycin there was a synergistic effect against Escherichia coli 06, and antagonism with norfloxacin.


Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Óleos Voláteis/farmacologia , Piper/química , Antibacterianos/análise , Antibacterianos/química , Antifúngicos/análise , Antifúngicos/química , Candida albicans/efeitos dos fármacos , Sinergismo Farmacológico , Eritromicina/farmacologia , Escherichia coli/efeitos dos fármacos , Gentamicinas/farmacologia , Testes de Sensibilidade Microbiana , Óleos Voláteis/análise , Óleos Voláteis/química , Folhas de Planta/química , Staphylococcus aureus/efeitos dos fármacos
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