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1.
Poult Sci ; 99(2): 1084-1087, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32029144

RESUMO

The objective of the current study was to evaluate increasing levels of manganese hydroxychloride (MHC) in 45-wk-old white leghorn laying hens, using yolk and shell manganese (Mn) content as a potential marker for Mn concentration. A total of 80, 45-wk-old white leghorns were assigned to 6 dietary treatments, each consisting of 14 individually caged laying hens, with the exception of the reference diet containing 10 individually caged laying hens. The experiment consisted of a reference diet that contained 70 ppm of supplemental inorganic Mn in the form of Mn oxide and 5 experimental treatments each containing 0, 15, 30, 60, and 90 ppm supplemental MHC. Experimental birds were subjected to a 21 D depletion phase in which no supplemental Mn was included in the diet; however, during this time reference fed birds were fed the control diet (70 ppm Mn). After the 21 D depletion phase, the depleted birds were fed experimental diets for a 35 D evaluation period. Yolk and shell Mn content were analyzed at the end of the depletion phase and during the experimental phase on day 5, 10, 15, 25, and 35. During the experimental phase, Mn was replenished in the yolk and shell in all experimental treatments containing supplemental Mn; however, dose and time impacted the rate of replenishment. The yolk tended to be more sensitive to variations in Mn level as increases in Mn inclusion significantly (P < 0.05) increased concentration. These data demonstrate the ability to deplete and replenish Mn, and the use of egg yolk Mn concentration as measurement for determining changes in dietary Mn. At the conclusion of the experiment at 35 D, 60 ppm of Mn hydroxychloride seemed to be adequate in replenishing Mn to the level of the reference.


Assuntos
Galinhas/metabolismo , Casca de Ovo/química , Gema de Ovo/química , Manganês/metabolismo , Ração Animal/análise , Animais , Dieta/veterinária , Suplementos Nutricionais/análise , Relação Dose-Resposta a Droga , Feminino , Manganês/administração & dosagem , Compostos de Manganês/administração & dosagem , Compostos de Manganês/metabolismo , Óxidos/administração & dosagem , Óxidos/metabolismo
2.
Oral Dis ; 26(2): 341-349, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31710760

RESUMO

OBJECTIVES: To determine glucose transporter 1 (GLUT1) and runt-related transcription factor 2 (RUNX2) expression during reparative dentinogenesis after pulpotomy with mineral trioxide aggregate (MTA) capping. SUBJECTS AND METHODS: Eight-week-old male Wistar rats were used. Pulp of the upper left first molar was exposed and capped with MTA. The upper right first molar of the same animal was used as a control. After collecting molars at various time points, GLUT1, RUNX2 and mammalian target of rapamycin (MTOR) were examined by immunohistochemistry. mRNA levels of Slc2a1 (encoding GLUT1), Runx2, Nestin and Mtor were determined by real-time PCR. RESULTS: Pulp exhibited progressive formation of reparative dentine lined with GLUT1- and MTOR-immunoreactive odontoblast-like cells at 5 days after pulpotomy. RUNX2 was detected in nuclei of most pulp tissue cells at day 5 after pulpotomy. Double immunofluorescence staining revealed GLUT1 immunoreactivity on odontoblast-like cells positive for Nestin or RUNX2, 5 days after pulpotomy. Slc2a1, Runx2, Nestin and Mtor mRNA levels were significantly upregulated on days 3-5 after pulpotomy. CONCLUSIONS: After rat molar pulpotomy, dental pulp induced formation of reparative dentine with colocalization of GLUT1 and Nestin or RUNX2. Moreover, mRNA levels of Slc2a1, Runx2, Nestin and Mtor were significantly upregulated in pulpotomized dental pulp.


Assuntos
Compostos de Alumínio/administração & dosagem , Compostos de Cálcio/administração & dosagem , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Capeamento da Polpa Dentária/métodos , Polpa Dentária/fisiologia , Dentinogênese/genética , Transportador de Glucose Tipo 1/genética , Óxidos/administração & dosagem , Pulpotomia , Silicatos/administração & dosagem , Serina-Treonina Quinases TOR/genética , Animais , Combinação de Medicamentos , Expressão Gênica , Imunoquímica , Masculino , Dente Molar/cirurgia , Nestina/genética , Odontoblastos/fisiologia , Ratos , Ratos Wistar
3.
Food Chem Toxicol ; 136: 110954, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31707033

RESUMO

Due to the growing number of applications of cadmium oxide nanoparticles (CdO NPs), there is a concern about their potential deleterious effects. The objective of our study was to investigate the effect of CdO NPs on the immune response, renal and intestine oxidative stress, blood antioxidant defence, renal fibrotic response, bone density and mineral content. Six-week-old female ICR mice were exposed to CdO NPs for 6 weeks by inhalation (particle size: 9.82 nm, mass concentration: 31.7 µg CdO/m3, total deposited dose: 0.195 µg CdO/g body weight). CdO NPs increased percentage of thymus CD3e+CD8a+ cells and moderately enhanced splenocyte proliferation and production of cytokines and chemokines. CdO NPs elevated pro-fibrotic factors (TGF-ß2, α-SMA and collagen I) in the kidney, and concentrations of AGEs in the intestine. The ratio of GSH and GSSG in blood was slightly reduced. Exposure to CdO NPs resulted in 10-fold higher Cd concentration in tibia bones. No differences were found in bone mass density, mineral content, bone area values, bone concentrations of Ca, P, Mg and Ca/P ratio. Our findings indicate stimulation of immune/inflammatory response, oxidative stress in the intestine, starting fibrotic response in kidneys and accumulation of CdO NPs in bones of mice.


Assuntos
Compostos de Cádmio/toxicidade , Fibrose/induzido quimicamente , Imunidade Celular/efeitos dos fármacos , Nanopartículas Metálicas/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Óxidos/toxicidade , Tíbia/efeitos dos fármacos , Administração por Inalação , Animais , Compostos de Cádmio/administração & dosagem , Citocinas/metabolismo , Feminino , Intestinos/efeitos dos fármacos , Rim/efeitos dos fármacos , Rim/patologia , Linfonodos/efeitos dos fármacos , Nanopartículas Metálicas/administração & dosagem , Camundongos Endogâmicos ICR , Óxidos/administração & dosagem , Baço/efeitos dos fármacos , Timo/efeitos dos fármacos
5.
Biomater Sci ; 7(11): 4491-4502, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31566646

RESUMO

Here, we have compared the behavioral neurotoxicity of a manganese nanoformulation (citrate functionalized Mn3O4 nanoparticles; C-Mn3O4 NPs) with that of the well-known neurotoxicant, ionic Mn, in an animal model. We found that mice administered with C-Mn3O4 NPs showed no signs of a neurobehavioral disorder, but the NPs instead ameliorated Mn-induced neurotoxicity (Parkinson's-like syndrome) through the chelation of excess Mn ions and subsequent reduction of oxidative damage.


Assuntos
Comportamento Animal/efeitos dos fármacos , Modelos Animais de Doenças , Hipocampo/efeitos dos fármacos , Nanopartículas/toxicidade , Neurônios/efeitos dos fármacos , Óxidos/toxicidade , Animais , Hipocampo/metabolismo , Hipocampo/patologia , Compostos de Manganês/administração & dosagem , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Nanopartículas/administração & dosagem , Neurônios/metabolismo , Neurônios/patologia , Oxirredução , Óxidos/administração & dosagem
6.
Biomater Sci ; 7(11): 4651-4660, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31464303

RESUMO

A traditional CuWO4 semiconductor (Eg = 2.25 eV) shows photoabsorption in the visible range with an edge at ∼550 nm, limiting its application in near-infrared (NIR) laser-induced photothermal ablation therapy (PAT). To tune the NIR photoabsorption of CuWO4, we report a trisodium citrate-induced strategy for generating oxygen vacancies. CuWO4 and CuWO4-x nanoparticles are prepared by a facile coprecipitation-solvothermal method in the absence or presence of trisodium citrate (0.2-0.5 g) as the reducing agent. Without trisodium citrate, CuWO4 consists of aggregated particles, and its dispersion has a typical yellow-green color without NIR photoabsorption. With the addition of trisodium citrate from 0.2 to 0.5 g, CuWO4-x samples are composed of monodisperse nanodots with increased sizes from ∼2 to ∼6 nm, and the color of these dispersions becomes darker with increased NIR photoabsorption, that is, from 0.178 to 0.685 at 808 nm. As a result, the aqueous dispersion of CuWO4-x-0.5 nanodots prepared with 0.5 g trisodium citrate exhibits a high photothermal efficiency of 47.6% under 808 nm laser irradiation. Simultaneously, CuWO4-x-0.5 nanodots have high X-ray attenuation as a CT imaging agent due to the presence of a heavy metal element (W). When the CuWO4-x-0.5 dispersion is injected into the tumors of mice, the tumors can be observed by CT and thermal imaging. After 808 nm laser irradiation (1.0 W cm-2, 10 min), cancer cells in vivo can be efficiently ablated by the photothermal effects of CuWO4-x, without obvious toxicity and side effects. Therefore, CuWO4-x can act as a novel all-in-one agent for CT imaging-guided photothermal therapy of tumors.


Assuntos
Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/terapia , Cobre/química , Nanoestruturas/química , Óxidos/química , Fototerapia , Tomografia Computadorizada por Raios X , Tungstênio/química , Animais , Cobre/administração & dosagem , Feminino , Raios Infravermelhos , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Nanoestruturas/administração & dosagem , Óxidos/administração & dosagem , Oxigênio/química , Processos Fotoquímicos , Tungstênio/administração & dosagem
7.
Food Nutr Bull ; 40(3): 357-368, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31272212

RESUMO

BACKGROUND: In order to improve calcium status, fortified rice should have acceptable organoleptic properties of that food. OBJECTIVE: We aimed to assess whether home fortification of rice with slaked lime can increase calcium content of rice and whether this fortified rice is well tolerated in a nutritionally at-risk population. METHODS: This experimental study measured the calcium content of rice cooked with different concentration of lime and assessed the acceptability of fortified rice among 400 women and children. Each participant received fortified rice with one of five concentrations of lime (0, 2.5, 5, 7.5 or 10 gm per 500 gm of rice), with or without additional foods (lentil soup or fried green papaya). All participants were asked to score the organoleptic qualities on a hedonic scale. RESULTS: Analysis showed that rice calcium content increased in a dose- response manner with increased lime during cooking (76.03, 205.58, 427.55, 614.29 and 811.23 mg/kg for given lime concentrations). Acceptability of the meal was greater when additional foods were served with rice at all lime concentrations. In both groups, the 7.5M arm reported highest overall acceptability (children, 6.25; women 6.10). This study found significant association between overall acceptability (different concentrations of lime mixed rice; with/without additional foods) and between groups (women vs. children) (p value = < 0.001) where-as no association was found within groups. CONCLUSIONS: Lime-fortified rice can be feasible considering the calcium uptake of rice and organoleptic character. Further research on bioavailability can establish a solid foundation that will support design of an effective intervention to reduce calcium deficiency in this population.


Assuntos
Compostos de Cálcio/administração & dosagem , Cálcio/deficiência , Oryza , Óxidos/administração & dosagem , Bangladesh , Disponibilidade Biológica , Cálcio/análise , Cálcio/farmacocinética , Criança , Comportamento do Consumidor , Feminino , Alimentos Fortificados/análise , Humanos , Oryza/química , Sensação
8.
J Colloid Interface Sci ; 553: 567-580, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31238227

RESUMO

Traditional cancer therapies carry a risk of serious side effects and toxicity. Developing an alternative treatment modality that is highly effective, has low toxicity and is noninvasive is urgently required. Here, we exploited molybdenum oxide (MoOx) nanosheets as a drug carrier and degradable photothermal agent to provide a chemo-photothermal combination cancer therapy. The MoOx nanosheets were synthesized by a one-pot hydrothermal method and then modified with pluronic F127 to improve physiological stability and biocompatibility. The F127-modified nanosheets (MoOX@F127) showed ultrahigh drug loading efficiency (DLE) of doxorubicin (DOX) (DLE%; 65%, W(load DOX)/[W(load DOX) + WMoOx@F127]), strong near-infrared (NIR) absorption and desirable pH-dependent degradability. After intravenous injection, MoOx@F127 nanosheets were degraded at physiological pH and were rapidly excreted from normal organs, while they were effectively accumulated and retained long-term in the more acidic tumor tissue. This simultaneously ensured effective tumor ablation after NIR irradiation and avoided long-term retention and toxicity in vivo. Compared to chemotherapy or photothermal therapy alone, in vitro and in vivo tumor ablation studies have shown a notably improved synergistic effect of the combination therapy. Our study presents a multifunctional nanosystem with a desirable degradability for chemo-photothermal combination cancer therapy that has great potential in biomedical applications.


Assuntos
Antibióticos Antineoplásicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Doxorrubicina/farmacologia , Nanopartículas/química , Fototerapia , Animais , Antibióticos Antineoplásicos/administração & dosagem , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Doxorrubicina/administração & dosagem , Feminino , Humanos , Concentração de Íons de Hidrogênio , Células MCF-7 , Masculino , Neoplasias Mamárias Experimentais/tratamento farmacológico , Neoplasias Mamárias Experimentais/patologia , Camundongos , Camundongos Endogâmicos BALB C , Molibdênio/administração & dosagem , Molibdênio/química , Óxidos/administração & dosagem , Óxidos/química , Tamanho da Partícula , Poloxâmero/administração & dosagem , Poloxâmero/química , Ratos , Ratos Wistar , Propriedades de Superfície , Células Tumorais Cultivadas
9.
Gen Dent ; 67(3): 47-51, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31199744

RESUMO

Mineral trioxide aggregate (MTA) pulpotomy may be an alternative to root canal therapy, with reported success rates as high as 85%. However, little technique-specific information has been reported regarding MTA placement in 1 visit. The purpose of this study was to evaluate different placement methods for MTA and resin-modified glass ionomer (RMGI) cement before immediate restoration with amalgam. Forty pulpotomized extracted third molars were randomly divided into 4 groups, and moist cotton was used to simulate pulp tissue in all teeth. In group 1, cotton was placed over the entire pulp chamber floor and in each canal orifice, and MTA was placed over the cotton. The procedure for group 2 was the same as that for group 1 except that a layer of RMGI was placed over the MTA. In group 3, cotton was placed in the canal orifices only, a layer of MTA was placed only over the cotton in the orifices, and RMGI was layered over the MTA and pulp chamber floor. The procedure for group 4 was the same as that for group 3 except that RMGI was placed over the MTA but not on the pulpal floor. Each of these procedures was followed by amalgam condensation. After a 7-day setting period, restored teeth were sectioned mesiodistally, photographed, measured, and evaluated for disturbance of the MTA-restoration junction. The study findings showed that the MTA layer was disturbed in 40% of the specimens in group 1, whereas 10%-20% of specimens in groups 2 through 4 demonstrated disturbed MTA. Analysis with a Pearson chi-square test indicated that the difference between group 1 and groups 2 through 4 was statistically significant (P < 0.05), but there was no significant difference (P > 0.05) between groups 2, 3, and 4. Group 3, in which MTA was placed over each canal orifice and RMGI was placed over the entire pulpal floor, performed best--only 10% of specimens exhibited deformed MTA. The findings suggest that RMGI may protect initially placed MTA during amalgam condensation.


Assuntos
Compostos de Alumínio/administração & dosagem , Compostos de Cálcio/administração & dosagem , Restauração Dentária Permanente/métodos , Óxidos/administração & dosagem , Pulpotomia , Materiais Restauradores do Canal Radicular , Silicatos/administração & dosagem , Combinação de Medicamentos , Humanos , Pulpotomia/instrumentação , Pulpotomia/métodos , Resultado do Tratamento
10.
Int J Pharm ; 566: 40-45, 2019 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-31129340

RESUMO

Photothermal therapy had great potential in being a new approach of tumour ablation due to their high selectivity and low side effect. However, the shallow penetration depth of near-infrared (NIR) irradiation resulted in the limited curative effect. Herein, a novel nanomedicine was developed based on the indocyanine green-loaded vanadium oxide nanocomposites (VO2-ICG) for pH-activated NIR luminescence imaging-guided enhanced photothermal tumour ablation. In acidic tumour microenvironment, the VO2 NPs were decomposed and released VO2+, which could not only inhibit the function of 60 kDa heat shock protein (HSP60), but also generate hydroxyl radical (OH) by catalysing intratumoral H2O2. Furthermore, the ICG was also released in the decomposition process of VO2 NPs, allowing the pH-activated NIR luminescence imaging and photothermal therapy. The inhibition of HSP60 down-regulated the heat tolerance of cells and the generation of OH up-regulated the intracellular oxidative stress, which enhanced the photothermal therapeutic efficiency. Our work demonstrated a promised method to enhance photothermal therapeutic effect, highlighting the importance of HSP inhibition and OH generation in promoting cell apoptosis under mild hyperthermia.


Assuntos
Chaperonina 60/antagonistas & inibidores , Hidróxidos/metabolismo , Verde de Indocianina/administração & dosagem , Nanocompostos/administração & dosagem , Neoplasias/terapia , Óxidos/administração & dosagem , Fototerapia , Compostos de Vanádio/administração & dosagem , Animais , Peróxido de Hidrogênio/metabolismo , Concentração de Íons de Hidrogênio , Luminescência , Camundongos Nus , Neoplasias/metabolismo , Espectroscopia de Luz Próxima ao Infravermelho
11.
J Am Soc Nephrol ; 30(6): 1073-1085, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31036759

RESUMO

BACKGROUND: Developing strategies for managing coronary artery calcification (CAC) in patients with CKD is an important clinical challenge. Experimental studies have demonstrated that magnesium inhibits vascular calcification, whereas the uremic toxin indoxyl sulfate aggravates it. METHODS: To assess the efficacy of magnesium oxide (MgO) and/or the oral carbon adsorbent AST-120 for slowing CAC progression in CKD, we conducted a 2-year, open-label, randomized, controlled trial, enrolling patients with stage 3-4 CKD with risk factors for CAC (diabetes mellitus, history of cardiovascular disease, high LDL cholesterol, or smoking). Using a two-by-two factorial design, we randomly assigned patients to an MgO group or a control group, and to an AST-120 group or a control group. The primary outcome was percentage change in CAC score. RESULTS: We terminated the study prematurely after an interim analysis with the first 125 enrolled patients (of whom 96 completed the study) showed that the median change in CAC score was significantly smaller for MgO versus control (11.3% versus 39.5%). The proportion of patients with an annualized percentage change in CAC score of ≥15% was also significantly lower for MgO compared with control (23.9% versus 62.0%). However, MgO did not suppress the progression of thoracic aorta calcification. The MgO group's dropout rate was higher than that of the control group (27% versus 17%), primarily due to diarrhea. The percentage change in CAC score did not differ significantly between the AST-120 and control groups. CONCLUSIONS: MgO, but not AST-120, appears to be effective in slowing CAC progression. Larger-scale trials are warranted to confirm these findings.


Assuntos
Carbono/administração & dosagem , Doença da Artéria Coronariana/diagnóstico , Doença da Artéria Coronariana/epidemiologia , Óxido de Magnésio/administração & dosagem , Óxidos/administração & dosagem , Calcificação Vascular/tratamento farmacológico , Calcificação Vascular/epidemiologia , Administração Oral , Idoso , Comorbidade , Doença da Artéria Coronariana/prevenção & controle , Progressão da Doença , Feminino , Hospitais Universitários , Humanos , Masculino , Pessoa de Meia-Idade , Cooperação do Paciente/estatística & dados numéricos , Prevenção Primária , Prognóstico , Valores de Referência , Insuficiência Renal Crônica/diagnóstico , Insuficiência Renal Crônica/epidemiologia , Insuficiência Renal Crônica/terapia , Índice de Gravidade de Doença , Resultado do Tratamento , Calcificação Vascular/prevenção & controle
12.
Pediatr Dent ; 41(2): 107-111, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30992107

RESUMO

Purpose: The purpose of this study was to compare the success of pulpotomies in primary molars using a new type of mineral trioxide aggregate (MTA; NeoMTA Plus) with a conventional MTA (ProRoot MTA) as a pulpotomy medicament in primary molars. Methods: Eighty primary teeth in 28 patients were divided randomly, with 40 teeth in a control group (ProRoot MTA) and 40 teeth in an experimental group (NeoMTA Plus). A standardized pulpotomy technique was performed for each tooth. Clinical and radiographic follow-up examinations were conducted at three, six, and 12 months. Results: At 12 months, the clinical success for ProRoot MTA was 97.4 percent (38 out of 39) and the radiographic success was 94.9 percent (37 out of 39); for NeoMTA Plus, the clinical success was 100 percent (40 out of 40) and the radiographic success was 97.5 percent (39 out of 40). No significant differences were found between the two groups at all follow-up evaluations. Conclusions: NeoMTA Plus showed a high percent success, similar to that of ProRoot MTA. NeoMTA Plus is a potential pulpotomy medicament for primary teeth.


Assuntos
Compostos de Alumínio/administração & dosagem , Compostos de Cálcio/administração & dosagem , Cimentos Dentários , Dente Molar , Óxidos/administração & dosagem , Pulpotomia/métodos , Silicatos/administração & dosagem , Dente Decíduo , Criança , Doenças da Polpa Dentária/etiologia , Combinação de Medicamentos , Feminino , Humanos , Masculino , Reabsorção da Raiz
13.
Cardiovasc Drugs Ther ; 33(3): 277-286, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30903544

RESUMO

PURPOSE: Several lines of evidence suggest that renal dysfunction is associated with cardiovascular toxicity through the action of uremic toxins. The levels of those uremic toxins can be reportedly reduced by the spherical carbon adsorbent AST-120. Because heart failure (HF) causes renal dysfunction by low cardiac output and renal edema, the removal of uremic toxins could be cardioprotective. METHOD: To determine whether blood levels of the uremic toxin indoxyl sulfate (IS) increase in HF and whether AST-120 can reduce those levels and improve HF. We induced HF in 12 beagle dogs by 6 weeks of rapid right ventricular pacing at 230 beats per min. We treated six dogs with a 1-g/kg/day oral dosage of AST-120 for 14 days from week 4 after the start of rapid ventricular pacing. The other six dogs did not receive any treatment (control group). RESULTS: In the untreated dogs, IS levels increased as cardiac function deteriorated. In contrast, plasma IS levels in the treated dogs decreased to baseline levels, with both left ventricular fractional shortening and pulmonary capillary wedge pressure also improving when compared with untreated dogs. Finally, AST-120 treatment was shown to reduce both myocardial apoptosis and fibrosis along with decreases in extracellular signal-regulated kinase phosphorylation, the Bax/Bcl-2 ratio, and TGF-ß1 expression and increases in AKT phosphorylation. CONCLUSIONS: IS levels are increased in HF. AST-120 treatment reduces the levels of IS and improves the pathophysiology of HF in a canine model. AST-120 could be a novel candidate for the treatment of HF.


Assuntos
Carbono/administração & dosagem , Síndrome Cardiorrenal/terapia , Insuficiência Cardíaca/terapia , Indicã/sangue , Nefropatias/prevenção & controle , Óxidos/administração & dosagem , Desintoxicação por Sorção/métodos , Uremia/prevenção & controle , Adsorção , Animais , Apoptose , Proteínas Reguladoras de Apoptose/metabolismo , Síndrome Cardiorrenal/sangue , Síndrome Cardiorrenal/fisiopatologia , Estado de Consciência , Modelos Animais de Doenças , Cães , Fibrose , Insuficiência Cardíaca/sangue , Insuficiência Cardíaca/complicações , Insuficiência Cardíaca/fisiopatologia , Hemodinâmica , Nefropatias/sangue , Nefropatias/etiologia , Nefropatias/fisiopatologia , Mitocôndrias Cardíacas/metabolismo , Mitocôndrias Cardíacas/patologia , Miocárdio/metabolismo , Miocárdio/patologia , Transdução de Sinais , Uremia/sangue , Uremia/etiologia , Uremia/fisiopatologia , Função Ventricular Esquerda
14.
Indian J Dent Res ; 30(1): 31-36, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30900653

RESUMO

Context: It is important to develop new therapeutic materials that have requisite clinical actions, are safe and economical. Aims: This study aims to histologically evaluate curcumin, an extract of turmeric (Curcuma longa) as a pulpotomy agent in rat molars and to compare it to mineral trioxide aggregate (MTA). Settings and Design: Animal study. Subjects and Methods: Twelve Wistar-Albino rats were randomly divided into two groups of 6 each. Pulpotomies were performed on caries free maxillary first and second molars on both sides of the arch, with MTA and curcumin (24 teeth each), respectively. Access cavities were sealed with resin-modified glass ionomer cement. Postoperative histological evaluation of pulpotomized teeth in both groups was done at 7, 14, and 30 days under a light microscope (×10). Statistical Analysis Used: Data were evaluated with Freidman's test and Mann-Whitney test at 0.05 level. Results: (a) There was a gradual reduction in inflammatory cell response in both groups across time periods tested (MTA P = 0.074, curcumin P = 0.039). (b) The overall architecture of pulp was maintained better in the curcumin group across all time periods tested (P = 0.368). (c) Dentinal bridge formation was consistently seen across time periods tested in MTA group (P = 0.9094) and was feeble in curcumin group (P = 0.9094) across time periods tested. Conclusions: Curcumin has been shown to have wound healing properties. It has the potential to be developed into a predictable and cost-effective vital pulp therapy medicament.


Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Curcumina/farmacologia , Polpa Dentária/patologia , Polpa Dentária/fisiologia , Óxidos/farmacologia , Agentes de Capeamento da Polpa Dentária e Pulpectomia/farmacologia , Pulpotomia/métodos , Silicatos/farmacologia , Cicatrização/efeitos dos fármacos , Compostos de Alumínio/administração & dosagem , Animais , Compostos de Cálcio/administração & dosagem , Curcumina/administração & dosagem , Combinação de Medicamentos , Óxidos/administração & dosagem , Ratos Wistar , Silicatos/administração & dosagem
15.
Braz Dent J ; 30(1): 22-30, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30864642

RESUMO

The purpose of this study was to evaluate the inflammatory process following direct pulp capping during pregnancy. This experimental study involved 48 maxillary first molars of female Wistar rats. The procedures were performed in pregnant and non-pregnant animals (n =20 each). Direct pulp capping with mineral trioxide aggregate (MTA) and restoration with a light-cured resin composite was performed in half of exposed pulp specimens. In the other half of specimens, light-cured composite was placed directly on the exposed pulp. In the control groups (n=4 each), no intervention was performed. Animals were euthanized at 3 and 7 days. All sections (three per slide) were viewed under an optical microscope. One previously calibrated pathologist performed descriptive analysis and assigned scores for inflammatory response and tissue organization adjacent to the pulp exposure. The Kappa value for intra-examiner variability was 0.91. At 3 days, in animals treated with MTA, inflammatory infiltrate was absent in non-pregnant animals while mild inflammatory infiltrate was observed in some pregnant animals. The inflammatory response ranged from mild to severe in both groups treated with composite alone. At 7 days, the inflammatory response was more intense in pregnant than in non-pregnant animals treated with MTA; while this difference were not evident in animals treated with composite alone. In conclusion, pregnancy may not influence the inflammatory process following direct pulp capping with light-cured resin composite, which was always harmful to the pulp; while the tissue response after the direct pulp with MTA were more favorable in non-pregnant animals.


Assuntos
Capeamento da Polpa Dentária/efeitos adversos , Inflamação/etiologia , Compostos de Alumínio/administração & dosagem , Animais , Compostos de Cálcio/administração & dosagem , Combinação de Medicamentos , Feminino , Óxidos/administração & dosagem , Gravidez , Ratos Wistar , Tratamento do Canal Radicular , Silicatos/administração & dosagem
16.
Sci Total Environ ; 665: 606-616, 2019 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-30776632

RESUMO

Plant response to microelements exposure can be modulated based on particle size. However, studies are lacking on the roles of particle size and specific microelements in mixed exposure systems designed for plant nutrition, rather than toxicology. Here, an addition-omission strategy was used to address particle-size and element-specific effects in soybean exposed to a mixture of nano and bulk scale oxide particles of Zn (2 mg Zn/kg), Cu (1 mg Cu/kg) and B (1 mg B/kg) in soil. Compared to the control, mixtures of oxide particles of both sizes significantly (p < 0.05) promoted grain yield and overall (shoot and grain) Zn accumulation, but suppressed overall P accumulation. However, the mixed nano-oxides, but not the mixed bulk-oxides, specifically stimulated shoot growth (47%), flower formation (63%), shoot biomass (34%), and shoot N (53%) and K (42%) accumulation. Compared by particle size, omission of individual elements from the mixtures evoked significant responses that were nano or bulk-specific, including shoot growth promotion (29%) by bulk-B; inhibition (51%) of flower formation by nano-Cu; stimulation (57%) of flower formation by bulk-B; grain yield suppression (40%) by nano-Zn; B uptake enhancement (34%) by bulk-Cu; P uptake stimulation by nano-Zn (14%) or bulk-B (21%); residual soil N (80%) and Zn (42%) enhancement by nano-Cu; and residual soil Cu enhancement by nano-Zn (72%) and nano-B (62%). Zn was responsible for driving the agronomic (biomass and grain yield) responses in this soil, with concurrent ramifications for environmental management (N and P) and human health (Zn nutrition). Overall, compared to bulk microelements, nanoscale microelements played a greater role in evoking plant responses.


Assuntos
Produção Agrícola/métodos , Nanopartículas/administração & dosagem , Material Particulado/administração & dosagem , Soja/efeitos dos fármacos , Boro/administração & dosagem , Cobre/administração & dosagem , Nanopartículas Metálicas/administração & dosagem , Micronutrientes/administração & dosagem , Óxidos/administração & dosagem , Fenômenos Fisiológicos Vegetais/efeitos dos fármacos , Solo/química , Soja/fisiologia , Zinco/administração & dosagem
18.
J Ultrasound Med ; 38(7): 1865-1873, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30560581

RESUMO

OBJECTIVES: To investigate 3-dimensional subharmonic aided pressure estimation (SHAPE) for measuring intraplaque pressure and the pressure gradient across the plaque cap as novel biomarkers for potentially predicting plaque vulnerability. METHODS: Twenty-seven rabbits received a high-cholesterol diet for 2 weeks before a balloon catheter injury to denude the endothelium of the aorta, followed by 8 to 10 weeks of the high-cholesterol diet to create arteriosclerotic plaques. SHAPE imagings of the resulting plaques were performed 12, 16, and 20 weeks after injury using a LOGIQ 9 scanner with a 4D10L probe (GE Healthcare, Milwaukee, WI) before and during an infusion of Definity (Lantheus Medical Imaging, North Billerica, MA) and Sonazoid (GE Healthcare, Oslo, Norway). The ratios of the maximum subharmonic magnitudes at baseline and during the infusion were correlated with the intraplaque pressure and pressure gradient across the plaque cap obtained from direct measurements. RESULTS: Ten rabbits died prematurely after the balloon injury procedure or due to toxicity from the high-cholesterol diet, whereas 2 rabbits were excluded for other conditions. Five rabbits were scanned in the 12-, 16-, and 20-week groups, respectively. Even after 20 weeks, the plaques that developed were very small (mean ± SD, 0.9 ± 0.4 × 0.14 ± 0.05 cm). Definity performed better than Sonazoid in this application but still only achieved a moderate correlation with pressure across the plaque cap (Definity, r = -0.40; Sonazoid, r = 0.22) and intraplaque pressure (Definity, r = -0.19; Sonazoid, r = -0.11). CONCLUSIONS: Initial findings from plaque pressure estimation using 3-dimensional SHAPE technique showed only moderate correlations with reference standards, but that may be have been due to weaknesses in the animal model studied.


Assuntos
Pressão Sanguínea , Imageamento Tridimensional , Placa Aterosclerótica/diagnóstico por imagem , Ultrassonografia/métodos , Animais , Meios de Contraste/administração & dosagem , Compostos Férricos/administração & dosagem , Fluorcarbonetos/administração & dosagem , Aumento da Imagem/métodos , Ferro/administração & dosagem , Óxidos/administração & dosagem , Coelhos
19.
Animal ; 13(6): 1137-1144, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30378527

RESUMO

The hypothesis of the research was the assumption, that manganese nanoparticles can affect the body in the same way as macromolecules. Their smaller size and greater biological reactivity will potentially allow the Mn addition to the diet to be reduced and, consequently, less excretion of this element into the environment. The aim of the study was to determine whether the use of Mn nanoparticles would make it possible to reduce the level of this micronutrient added to turkey diets without adversely affecting redox reactions in cells and epigenetic changes. The experiment was conducted on six groups with 10 replications, in a two-factor design with three dosages of manganese: 100, 50 and 10 mg/kg, and two sources: manganese oxide (MnO) and manganese nanoparticles (NP-Mn2O3). Markers of oxidative stress determined in the blood, that is, the concentration of lipid hydroperoxides, malondialdehyde, protein carbonyl derivatives, 3-nitrotyrosine, 8-hydroxydeoxyguanosine, total glutathione, superoxide dismutase, glutathione peroxidase, catalase, ceruloplasmin, total antioxidant status, albumin and vitamin C content. The level of epigenetic changes in the blood was determined by analysing global DNA methylation. In the experiment, in which the diet of turkeys was supplemented with two forms of Mn (MnO or NP-Mn2O3) at three dosages: 100, 50 and 10 mg/kg, the 10 mg/kg dose, especially in the form of NP-Mn2O3, induced lipid oxidation reactions to the greatest extent. Irrespective of the dosage of Mn in the turkey diet, Mn in the form of NP-Mn2O3 was found to reduce protein nitration more than Mn in the form of MnO. Reducing the Mn dosage in the diet from 100 to 50 mg/kg and then to 10 mg/kg is unfavourable because proportionally increases protein and DNA oxidation in cells, decreases the activity of antioxidant enzymes, and increases the level of glutathione. Reducing the dosage from 100 to 10 mg/kg increases global DNA methylation. The reduction of the Mn level, regardless of the form used, is disadvantageous, because it weakens the defense of the antioxidant system, which consequently can induce oxidative processes in the cells. Although Mn in the form of NP-Mn2O3 reduce protein nitration better than in MnO form, the use of manganese nanoparticles in turkey feeding (even in lower doses) requires further study.


Assuntos
Dieta/veterinária , Epigênese Genética , Compostos de Manganês/farmacologia , Manganês/farmacologia , Nanopartículas Metálicas/química , Óxidos/farmacologia , Perus , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Antioxidantes/metabolismo , Biomarcadores , Suplementos Nutricionais , Relação Dose-Resposta a Droga , Regulação da Expressão Gênica/efeitos dos fármacos , Glutationa Peroxidase/metabolismo , Malondialdeído/metabolismo , Manganês/administração & dosagem , Manganês/química , Compostos de Manganês/administração & dosagem , Compostos de Manganês/química , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Óxidos/administração & dosagem , Óxidos/química , Superóxido Dismutase/metabolismo
20.
ACS Appl Mater Interfaces ; 11(3): 3323-3333, 2019 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-30589536

RESUMO

Black titanium oxide has attracted tremendous interest in tumor phototherapy via converting light energy to heat and reactive oxygen species (ROS). Nevertheless, current synthesis methods suffer from inert gas shielding, high costs, complicated procedures, and expensive facilities, which are fairly impractical for treatment application. Herein, we propose a one-step strategy for fast facile synthesis of black TiO nanoparticles via a microwave-assisted hydrothermal synthesis approach with Ti power, hydrochloric acid, and hydrofluoric acid without the requirement of an reducing agent and high-temperature calcination. The prepared black TiO nanoparticles with an average size of 52 nm exhibit strong absorbance from ultraviolet (UV) to near-infrared light region, favoring a single agent and single light-induced synergistic phototherapy of tumors. The black TiO nanoparticles shows an excellent performance in phototherapy with a photothermal conversion efficiency up to 50% and a prominent ROS generation under 808 nm laser irradiation. The toxicity and therapeutic effect in vitro and in vivo are investigated, and the results elucidate that black TiO nanoparticles possess good biocompatibility and remarkable synergistic tumor therapeutic efficacy. The proposed microwave-assisted method opens up a novel way for the synthesis of titanium-based material in a simple and fast manner, promoting their applications in the biomedical field.


Assuntos
Nanopartículas Metálicas/administração & dosagem , Neoplasias/terapia , Fototerapia , Titânio/administração & dosagem , Animais , Linhagem Celular Tumoral , Doxorrubicina/administração & dosagem , Doxorrubicina/química , Sinergismo Farmacológico , Temperatura Alta/uso terapêutico , Humanos , Nanopartículas Metálicas/química , Nanopartículas Metálicas/uso terapêutico , Camundongos , Micro-Ondas , Neoplasias/patologia , Óxidos/administração & dosagem , Óxidos/síntese química , Óxidos/química , Espécies Reativas de Oxigênio/química , Titânio/química , Ensaios Antitumorais Modelo de Xenoenxerto
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