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1.
J Appl Oral Sci ; 29: e20200448, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33787729

RESUMO

OBJECTIVE: Denture adhesives need complete removal due to their frequent replacement. Our study investigates the removal of denture adhesives from denture base materials, using different methods. METHODOLOGY: PMMA and Polyamide denture base materials were used to fabricate 120 samples (15×15×1.5mm). One side of the samples was left as processed and the other polished with a usual procedure, hydrated for 24 h, dried, and weighted. They received 0.2 g of three adhesive creams on their unpolished surface (Corega, Olivafix, Fittydent), pressed on polysulfide material, stored under 37°C and 95% rel. humidity for 1 h and 60 of them, following their separation from polysulfide base, brushed under running water, whereas the rest inserted in a cleanser bath (Fittydent Super) for 5 min. The samples were dried and inserted in the oven (37°C) for additional 10 min and weighted again. Roughness tests of denture materials and light microscopy of adhesives creams were also used to evaluate the materials. Time lapse images of spayed with water adhesives on PMMA base were also taken to evaluate the volumetric changes of adhesives. Weight data before and after adhesive removal, indicating the amount of remaining adhesive, were statistically analyzed using Welch's ANOVA and Games-Howell multiple comparisons tests at α=0.05 level of significance. RESULTS: Roughness of Polyamide was higher than PMMA and Fittydent showed greater volumetric changes than the others. Significant differences (p<0.05), were found between PMMA and Polyamide bases, between Olivafix and Fittydent adhesives, and between brushing and cleansing methods but only for PMMA-Olivafix combination. CONCLUSIONS: Adhesives showed a stronger adherence to PMMA surface, and Fittydent was the most difficult to be removed. Removal methods were not effective for all adhesives or denture base materials. These indicate that removal methods, adhesive type and denture base material are all playing a significant role in the removal of adhesives from denture surfaces.


Assuntos
Bases de Dentadura , Nylons , Adesivos , Teste de Materiais , Polimetil Metacrilato , Propriedades de Superfície
2.
Chem Biol Interact ; 339: 109445, 2021 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-33741339

RESUMO

Taxifolin (3,5,7,3,4-pentahydroxy flavanone or dihydroquercetin, Tax) was identified as a gastroprotective compound and a gastroadhesive formulation was recently developed to prolong its residence time and release in the stomach. So, the gastric healing effectiveness of Tax and gastro-mucoadhesive microparticles containing Tax (MPTax) against the acetic acid induced-gastric ulcer in rats was investigated in this study. Moreover, the interactions between Tax and H+/K+-ATPase were investigated in silico, and its anti- H. pylori activity was determined in vitro. The oral treatment with MPTax (81.37 mg/kg, containing 12.29% of Tax) twice a day for seven days reduced the ulcer area by 63%, compared to vehicle-treated group (Veh: 91.9 ± 10.3 mm2). Tax (10 mg/kg, p.o) reduced the ulcer by 40% but with a p = 0.07 versus Veh group. Histological analysis confirmed these effects. Tax and MPTax increased the gastric mucin amount, reduced the myeloperoxidase activity, and increased the glutathione reduced content at ulcer site. However, only MPTax decreased the lipoperoxide accumulation at ulcer site. Besides, Tax and MPTax normalize the catalase and glutathione S-transferase activity. Tax showed reversible interaction with H+/K+-ATPase in silico and its anti-H. pylori effects was confirmed (MIC = 625 µg/mL). These results suggest that the antiulcer property of Tax involves the strengthening of the gastric protective factors in parallel to its inhibitory interaction with H+/K+-ATPase and H. pylori. Considering that ulcer healing action displayed by Tax was favored by gastroadhesive microparticles, this approach seems to be promising for its oral delivery to treat acid-peptic diseases.


Assuntos
Adesivos/farmacologia , Helicobacter pylori/efeitos dos fármacos , Bombas de Próton/fisiologia , Quercetina/análogos & derivados , Estômago/efeitos dos fármacos , Cicatrização/efeitos dos fármacos , Ácido Acético/farmacologia , Animais , Antiulcerosos/farmacologia , Antioxidantes/metabolismo , Catalase/metabolismo , Simulação por Computador , Feminino , Mucinas Gástricas/metabolismo , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/metabolismo , ATPase Trocadora de Hidrogênio-Potássio/metabolismo , Infecções por Helicobacter/tratamento farmacológico , Infecções por Helicobacter/metabolismo , Infecções por Helicobacter/microbiologia , Fitoterapia/métodos , Extratos Vegetais/farmacologia , Quercetina/fisiologia , Ratos , Ratos Wistar , Úlcera Gástrica/induzido quimicamente , Úlcera Gástrica/tratamento farmacológico , Úlcera Gástrica/metabolismo , Úlcera Gástrica/microbiologia
3.
BMC Surg ; 21(1): 158, 2021 Mar 22.
Artigo em Inglês | MEDLINE | ID: mdl-33752652

RESUMO

BACKGROUND: Laparoscopic paraesophageal hernia repair is associated with higher recurrence rate. Mesh is used to reduce the recurrence rate. This retrospective study is to review our experience of biological mesh fixed with suture and medical glue in hiatal hernias repairs. METHODS: A retrospective chart review was conducted for a consecutive series of patients undergoing laparoscopic hiatal herniorrhaphy between January 2018 and January 2019. After hiatus closure, a piece of biological prosthesis was fixed with medical glue and suture for reinforcement of the crural closure. Clinical outcomes were reviewed, and data were collected regarding operative details, complications, symptoms, and follow-up imaging. Radiological evidence of any size of hiatal hernia was considered to indicate a recurrence. RESULTS: Thirty-six patients underwent surgery uneventfully without any serious complication. There was no mortality. The follow-up was, on average, 18.4 months, and there was no symptomatic recurrence. There was one anatomical recurrence without any related presentation. The method of mesh fixation with medical glue and suture took 12 min on average, and the handling was fairly easy. CONCLUSIONS: Biological mesh fixed with suture and medical glue was safe and effective for repairing large hiatal hernias. Of course, a longer follow-up is still needed for determining long-term outcomes.


Assuntos
Hérnia Hiatal , Herniorrafia , Laparoscopia , Adesivos , Idoso , Idoso de 80 Anos ou mais , Feminino , Hérnia Hiatal/cirurgia , Herniorrafia/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva , Estudos Retrospectivos , Telas Cirúrgicas , Suturas , Resultado do Tratamento
4.
AAPS PharmSciTech ; 22(3): 87, 2021 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-33665761

RESUMO

The commitment of the existent study was to develop a mucoadhesive in situ gel systems of vitamin B12 for the management of dry eye disease. The gels were prepared using pluronic F-127 and either of chitosan, carbapol 971P, sodium alginate, or hydroxy propyl methyl cellulose. Drug-excipients compatibility was investigated by means of differential scanning calorimetry and Fourier transform infrared spectroscopy. The gels were characterized for pH, clarity, gelling capacity, viscosity, and adhesion. In vitro release of vitamin B12 from the selected gels was investigated. In vivo effectiveness of the selected gel was determined in rabbit models using Schirmer's and fluorescein tests. The compatibility studies revealed the possibility of incidence of drug/polymer interaction in some formulations. F2-containing pluronic F127 and hydroxypropyl methyl cellulose showed the most appropriate physical characterization and in vitro release profile. The prepared gels showed prolonged drug release with drug release mechanism of combined diffusion and erosion. The in vivo study revealed good effectiveness of the prepared mucoadhesive in situ gel system of vitamin B12 in the treatment of dry eye disease that was comparable to that of the marketed drops.


Assuntos
Síndromes do Olho Seco/tratamento farmacológico , Vitamina B 12/uso terapêutico , Vitaminas/uso terapêutico , Adesivos , Animais , Composição de Medicamentos , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Excipientes , Feminino , Géis , Derivados da Hipromelose , Masculino , Membrana Mucosa , Poloxâmero , Coelhos , Viscosidade , Vitamina B 12/administração & dosagem , Vitaminas/administração & dosagem
5.
Eur J Oral Sci ; 129(2): e12768, 2021 04.
Artigo em Inglês | MEDLINE | ID: mdl-33667032

RESUMO

This study aimed at determining the enamel bond durability of universal adhesives to intact enamel (IE) and ground enamel (GE) in different etching modes under thermal cycling (TC) and fatigue stresses. Two universal adhesives and a two-step self-etch adhesive were used. Enamel specimens were divided into four groups by treatment: (i) GE in etch-and-rinse, (ii) IE in etch-and-rinse, (iii) GE in self-etch, and (iv) IE in self-etch. Bonded specimens were subjected to TC, and then shear bond strengths (SBS) were conducted. SBS tests were also conducted after storage in 37°C water for 24 h (baseline). For shear fatigue strength (SFS) testing, bonded specimens were subjected to fatigue stress at a frequency of 10 Hz for 50,000 cycles. At baseline, all adhesives showed significantly higher SBS values with GE than IE in self-etch mode in contrast to in etch-and-rinse mode. After TC, the universal adhesives with IE showed lower SBS values than the baseline, regardless of the etching mode. Universal adhesives showed significantly lower SFS values with IE in etch-and-rinse, but not in self-etch mode. Phosphoric acid pre-etching or grinding of the aprismatic enamel may be helpful in establishing reliable initial and long-term bonds to enamel when using universal adhesives in self-etch mode.


Assuntos
Condicionamento Ácido do Dente , Colagem Dentária , Adesivos , Cimentos Dentários , Esmalte Dentário , Adesivos Dentinários , Teste de Materiais , Cimentos de Resina , Resistência ao Cisalhamento
6.
Carbohydr Polym ; 261: 117878, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33766365

RESUMO

Hydrogels have gained great attentions as wound dressing. Binding to the tissue and preventing wound infection were the basic requirements for an "ideal dressing". We employed l-DOPA and ε-Poly-l-lysine to modify thermo-sensitive hydroxybutyl chitosan (HBC) to obtain (l-DOPA) - (ε-Poly-l-lysine)-HBC hydrogels (eLHBC). The eLHBC exhibited an almost 1.5 fold (P < 0.01) increase in wet adhesion strength compared to HBC. Upon the introduction of ε-Poly-l-lysine, eLHBC presented inherent antimicrobial property and prevented wound infection and inflammation response. Bone marrow mesenchymal stem cells (BMSCs) encapsulated in the eLHBC (BMSCs ⊂ eLHBC) could secret cytokins and growth factors via paracrine and promote the migration of fibroblast cells. BMSCs ⊂ eLHBC enhanced the complete skin-thickness wound healing via promoting collagen deposition and inhibiting infection and inflammation in vivo with wound closure rate being above 99 % after 15 days. The bioinspired, tissue-adhesive eLHBC could serve as advanced wound dressings for facilitating tissue repair and regeneration.


Assuntos
Adesivos , Curativos Hidrocoloides , Quitosana/análogos & derivados , Células-Tronco Mesenquimais/efeitos dos fármacos , Tecidos Suporte/química , Adesivos/síntese química , Adesivos/química , Adesivos/farmacologia , Animais , Antibacterianos/síntese química , Antibacterianos/química , Antibacterianos/farmacologia , Bioengenharia/métodos , Materiais Biomiméticos/síntese química , Materiais Biomiméticos/química , Materiais Biomiméticos/farmacologia , Bivalves/química , Bivalves/metabolismo , Adesão Celular/efeitos dos fármacos , Técnicas de Cultura de Células/instrumentação , Técnicas de Cultura de Células/métodos , Células Cultivadas , Quitosana/síntese química , Quitosana/química , Quitosana/farmacologia , Matriz Extracelular/química , Matriz Extracelular/efeitos dos fármacos , Hemólise/efeitos dos fármacos , Hidrogéis/síntese química , Hidrogéis/química , Hidrogéis/farmacologia , Masculino , Teste de Materiais , Células-Tronco Mesenquimais/citologia , Células-Tronco Mesenquimais/fisiologia , Camundongos , Testes de Sensibilidade Microbiana , Peptídeos/síntese química , Peptídeos/química , Peptídeos/farmacologia , Coelhos , Ratos , Ratos Sprague-Dawley , Temperatura , Cicatrização/efeitos dos fármacos
7.
Br J Surg ; 108(1): 14-23, 2021 01 27.
Artigo em Inglês | MEDLINE | ID: mdl-33640918

RESUMO

BACKGROUND: The optimal choice for mesh fixation in laparoscopic inguinal hernia repair (LIHR) has not been well established. This review compared the effects of glue versus mechanical mesh fixation in LIHR on incidence of chronic postoperative inguinal pain (CPIP) and other secondary outcomes, including acute pain, seroma, haematoma, hernia recurrence and other postoperative complications. METHODS: A systematic review of English/non-English studies using MEDLINE, the Cochrane Library, OpenGrey, OpenThesis and Web of Science, and searching bibliographies of included studies was completed. Search terms included laparoscopic, hernia, fibrin glue, Tisseel, Tissucol, cyanoacrylate, Glubran and Liquiband. Only RCTs comparing mechanical with glue-based fixation in adult patients (aged over 18 years) that examined CPIP were included. Two authors independently completed risk-of-bias assessment and data extraction against predefined data fields. All pooled analyses were computed using a random-effects model. RESULTS: Fifteen RCTs met the inclusion criteria; 2777 hernias among 2109 patients were assessed. The incidence of CPIP was reduced with use of glue-based fixation (risk ratio (RR) 0.36, 95 per cent c.i. 0.19 to 0.69; P = 0.002), with moderate heterogeneity that disappeared with sensitivity analysis (8 d.f.) for patient-blinded studies (RR 0.43, 0.27 to 0.86). Trial sequential analysis provided evidence for a relative risk reduction of at least 25 per cent. The incidence of haeamtoma was reduced by using glue-based fixation (RR 0.29, 0.10 to 0.82; P = 0.02) with no significant effects on seroma formation or hernia recurrence (RR 1.07, 0.46 to 2.47; P = 0.88). CONCLUSION: Glue-based mesh fixation appears to reduce the incidence of CPIP and haematoma after LIHR compared with mechanical fixation, with comparable recurrence rates.


Assuntos
Adesivos/uso terapêutico , Hérnia Inguinal/cirurgia , Herniorrafia/métodos , Laparoscopia/métodos , Telas Cirúrgicas , Herniorrafia/instrumentação , Humanos , Laparoscopia/instrumentação , Dor Pós-Operatória/epidemiologia , Ensaios Clínicos Controlados Aleatórios como Assunto
8.
Klin Lab Diagn ; 66(1): 45-51, 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33567173

RESUMO

The microbiome of oral cavity in healthy people and patients with periodontitis was analyzed to determine their adhesive properties and the ability to form biofilms. The study involved 2 groups: healthy, 18 people, and an experimental group, 20 patients with chronic generalized periodontitis moderate severity of the disease. The average age of the studied people was 35-45 years. Material - dental plaque, scraping from the mucous membrane of the back of the tongue, the contents of the periodontal groove and periodontal pocket, as well as oral fluid. The main method of diagnostic was bacteriological. The average adhesion index (AAI) was used to determine adhesion level of microorganisms to epithelial cells of oral cavity's mucous membrane. The microbiota's ability to form biofilm was tested on glass and plastic surface. The microbiota of oral cavity of patients with periodontitis was characterized by decrease in the frequency of bacteria of the genera: Streptococcus, Peptostreptococcus, Peptococcus, and an increase in Staphylococcus aureus, Veillonella spp., Bacillus spp. The microbiota of the oral cavity of patients with generalized periodontitis has a greater ability to adhere to the cells of the mucous membrane than in healthy people, while their ability to form biofilms and exhibit pathogenic properties is enhanced. The biofilm formation of microorganisms in healthy and sick people differs both on glass and on plastic surfaces.


Assuntos
Adesivos , Microbiota , Adulto , Biofilmes , Humanos , Pessoa de Meia-Idade , Bolsa Periodontal
9.
Hu Li Za Zhi ; 68(1): 74-81, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-33521921

RESUMO

BACKGROUND & PROBLEMS: Patients with critical illnesses face an elevated risk of medical adhesive relation skin injuries (MARSI), which have negative, subsequent impacts on recovery and quality of healthcare. PURPOSE: The aim of this project was to decrease the incidence of MARSI in the surgical intensive care unit and to improve the accuracy of MARSI preventive care implementation. RESOLUTIONS: The intervention included the implementation of product-use cards, high-risk warning slogans, education programs, experience workshops, and a standard prevention-care protocol for MARSI. RESULTS: After project implementation, the incidence rate of MARSI decreased from 18.2% to 0%-9.3%, and the accuracy rate of preventive care increased from 38.6% to 95.5%. CONCLUSIONS: This project effectively reduced the incidence of skin injury and improved the quality of critical care. The skills related to the care and prevention of MARSI have been implemented throughout the hospital.


Assuntos
Adesivos , Dermatopatias , Adesivos/efeitos adversos , Cuidados Críticos , Humanos , Incidência , Unidades de Terapia Intensiva , Pele , Dermatopatias/cirurgia
10.
Niger J Clin Pract ; 24(2): 282-291, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33605921

RESUMO

Aims: To evaluate the effect of various aging periods and different surface preparation methods on microtensile bond strength (µTBS) for composite repair. Materials and Methods: One hundred twelve composite resin blocks were formed using a nanohybrid composite resin. The samples were distributed into four groups according to surface preparation methods (n = 28): control (sound composite blocks); Er, Cr: YSGG laser; air abrasion; silicone carbide. All samples were then divided into four subgroups according to various aging periods: (i) No aging, (ii) 10,000 thermocycling, (iii) 30,000 thermocycling, and (iv) 50,000 thermocycling. Following surface preparation and aging procedures, surface topography of one sample from each group was evaluated under scanning electron microscope (SEM). The repair composites were bonded to the sample surfaces, using a three-step etch&rinse adhesive. Finally, thirty beams of size 1 × 1 × 8 mm from each group were subjected to µTBS test and failure modes were determined. The data were analyzed using two-way ANOVA, Post-hoc Bonferroni, and Chi-square tests (P = 0.05). Results: When different surface preparation methods were evaluated together, no aging and 10,000 thermocycling groups displayed higher µTBS values (P < 0.05). When all aging periods were evaluated together, the surface preparation with air abrasion provided higher µTBS (P < 0.05). The interactions of various aging periods with different surface preparation methods revealed significant variations in repair µTBS (P < 0.05). There were statistically significant differences on failure mode distributions among surface preparation methods (P < 0.001). SEM evaluations provided valuable outcomes that help to comment on the µTBS findings. Conclusions: Different surface preparation methods, various aging periods, and the interaction of both affected the repair µTBS of the tested nanohybrid composite resin.


Assuntos
Colagem Dentária , Lasers de Estado Sólido , Adesivos , Resinas Compostas , Análise do Estresse Dentário , Humanos , Teste de Materiais , Cimentos de Resina , Propriedades de Superfície , Resistência à Tração
11.
Otol Neurotol ; 42(4): 510-516, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-33443975

RESUMO

OBJECTIVES: The aim of this study was to evaluate the performance and limits of an adhesive bone conduction hearing aid in patients implanted with an active transcutaneous bone conduction implant. Therefore, hearing performance and subjective benefit of patients with mixed and conductive hearing loss were assessed with both bone conduction devices. STUDY DESIGN AND PATIENTS: This cohort study was conducted at a tertiary care center. Fifteen subjects, who had been implanted with an active transcutaneous device previously, were included and used the adhesive hearing device for 3 weeks instead of the implant. Subjects underwent two sets of audiological tests as well as assessments of quality of life at the beginning and at the end of the testing period. RESULTS: Audiological results showed a significantly greater improvement in regards to functional hearing gain and word recognition scores with the transcutaneous bone conduction device than the nonimplantable adhesive device. Regression analysis showed a trend toward greater improvement with the transcutaneous device compared with the adhesive device in patients with an increasing bone conduction threshold. Hearing-specific and general quality-of-life questionnaires revealed no significant difference between the two devices. CONCLUSION: Patients with mixed or conductive hearing loss experience hearing gain with both, the adhesive device and the active transcutaneous device. The adhesive device may be a valuable alternative to the active transcutaneous device, depending on the individual bone conduction threshold.


Assuntos
Auxiliares de Audição , Perda Auditiva Condutiva-Neurossensorial Mista , Percepção da Fala , Adesivos , Condução Óssea , Estudos de Coortes , Perda Auditiva Condutiva , Humanos , Qualidade de Vida , Resultado do Tratamento
12.
Life Sci ; 270: 119126, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33508295

RESUMO

AIMS: To investigate the biomimetic fabrication of fibrous-like organic-inorganic hybrid structures via a simple bottom-up approach, viz. self-assembly of simple molecules, and apply fibrous-like composites as a novel primer to improve dentin bond strengths of self-etch adhesives. MATERIALS AND METHODS: The resultants of commercial amorphous calcium phosphate (ACP) nanoparticles and 10-methacryloyloxydecyl dihydrogen phosphate (MDP) ethanol-aqueous solution were analyzed by TEM, SEM, XRD, DLS and AFM. The acid and alkali resistance of abovementioned self-assembled composites were analyzed with TEM. Micro-tensile bond strengths (MTBS) tests were performed after polished dentin surfaces were pretreated with self-assembled composites. The pretreated dentin surfaces and dentin-resin interfaces were characterized by SEM/TEM. KEY FINDINGS: ACP nanoparticles in MDP solution could self-assemble into fibrous-like nanotube structures in 8 nm diameter. Self-assembly and self-proliferation process went from ACP nanoparticles, dissolved ACP nanoparticles (less than 50 nm), twig-like structures and fibrous-like nanotubes to cellular networks. The fibrous-like nanotubes were only detected when the amount of ACP in reaction system were more than 0.01 g. The more ACP interacted with MDP, the more fibrous-like nanotubes were formed. After the dentin surfaces were treated with fibrous-like nanotube composites, MTBS could be significantly improved. Moreover, the fibrous-like nanotube structures could be resistant to acidic challenge, and were stable at least for 3 months. SIGNIFICANCE: The fibrous-like nanotube structures could be self-assembled via a bottom-up approach at certain ratio of MDP and commercial ACP nanoparticles. The application of fibrous-like nanotube composites as a novel primer prior to self-etch adhesives greatly improved dentin bond strengths.


Assuntos
Biomimética/métodos , Dentina/química , Nanotubos/química , Adesivos/química , Fosfatos de Cálcio/química , Humanos , Teste de Materiais/métodos , Metacrilatos/química , Nanopartículas/química , Resistência à Tração/fisiologia
13.
Spine (Phila Pa 1976) ; 46(4): E243-E249, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33475276

RESUMO

STUDY DESIGN: An in vitro experimental study testing a Gelatin-poly (γ-glutamic acid) hydrogel for disc repair. OBJECTIVE: To evaluate the cytocompatibility and degradability of the above mentioned hydrogel for intervertebral disc annular fibrosis (AF) repair. SUMMARY OF BACKGROUND DATA: No repair strategies for correcting annular defects in lumbar discectomy have been clinically well recognized. Exogenous supplementation of regenerative materials to fill defects is a minimally invasive way to restore compromised mechanical properties. The injected materials, most commonly gelatin-based materials with cross-linking agents, serve as sealants and as a scaffold for incorporating biomaterials for augmentation. However, cytotoxicity of hydrogel crosslinking agents is of concern in developing viable materials. METHODS: This in vitro experimental study evaluated a newly developed gelatin-based hydrogel for intervertebral disc AF repair. Mechanical strength was augmented by γ-PGA, and 1-(3-dimethylaminopropyl)-3-ethyl-carbodiimide hydrochloride (EDC) was used for material crosslinking. Isolated bovine tail intervertebral discs (IVDs) were used to test the hydrogel, and hydrogel surface monolayer AF cell culture was used to investigate efficacy in hydrogel constructs of different EDC concentrations. Cell metabolic activity was evaluated with Alamar blue assay, cell viability assay with live/dead stain, and sulfated glycosaminoglycan (GAG) and double strain DNA were quantified to evaluate proliferation of implanted cells and synthesis of extracellular matrix (ECM) proteins. RESULTS: EDC concentrations from 10 to 40 mM resulted in significant decreases in AF cell proliferation without obvious influence on cell viability. Higher EDC concentrations resulted in decreased percentage of Alamar blue reduction and GAG and DNA concentration, but did not affect GAG/DNA and live-dead ratios. Degradation tests revealed that higher EDC concentrations decreased the hydrogel degradation rate. CONCLUSION: The developed gelatin-poly (γ-PGA) hydrogel with 20 mM EDC concentration provides an effective gap-filling biomaterial with good cytocompatibility, suggesting substantial promise for use as a sealant for small AF defects.Level of Evidence: N/A.


Assuntos
Adesivos/uso terapêutico , Gelatina/farmacologia , Ácido Glutâmico/farmacologia , Degeneração do Disco Intervertebral/tratamento farmacológico , Disco Intervertebral/efeitos dos fármacos , Animais , Anel Fibroso/cirurgia , Materiais Biocompatíveis , Bovinos , Células Cultivadas , Discotomia , Ácido Glutâmico/metabolismo , Glicosaminoglicanos , Hidrogéis , Disco Intervertebral/cirurgia , Degeneração do Disco Intervertebral/cirurgia , Ácido Poliglutâmico/análogos & derivados
14.
Ear Nose Throat J ; 100(2_suppl): 148S-151S, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33410706

RESUMO

Patients with severe coronavirus disease 2019 (COVID-19) may have endothelial inflammation, pseudoaneurysm, and an increasing risk of bleeding, especially during surgical procedures. In this article, we reported 2 cases of COVID-19 patients with neck vascular lesions. The first patient had pseudoaneurysm of the cricothyroid artery, which was treated by percutaneous glue injection through ultrasonography guidance. The second patient presented lateral neck hematoma in front of the left superior thyroid artery, which was managed by coil endovascular embolization. In the context of pandemic, the management of vascular lesions may be performed through interventional radiological procedures that may reduce the risk of virus aerosolization and health care provider contamination.


Assuntos
Adesivos/uso terapêutico , Falso Aneurisma/terapia , Embolização Terapêutica/métodos , Hematoma/terapia , Complicações Pós-Operatórias/terapia , Radiologia Intervencionista , Traqueotomia , Idoso , Falso Aneurisma/complicações , Falso Aneurisma/diagnóstico por imagem , Angiografia por Tomografia Computadorizada , Cianoacrilatos/uso terapêutico , Procedimentos Endovasculares , Hematoma/complicações , Hematoma/diagnóstico por imagem , Humanos , Masculino , Pessoa de Meia-Idade , Pescoço , Complicações Pós-Operatórias/diagnóstico por imagem , Glândula Tireoide/irrigação sanguínea , Ultrassonografia
15.
Biochem J ; 478(1): 61-62, 2021 01 15.
Artigo em Inglês | MEDLINE | ID: mdl-33417681

RESUMO

Xanthophylls are coloured isoprenoid metabolites synthesized in many organisms with a variety of functions from the attraction of animals for impollination to absorption of light energy for photosynthesis to photoprotection against photooxidative stress. The finding by Proctor and co-workers makes a new addition to the last type of functions by showing that zeaxanthin is instrumental in coordinating chlorophyll biosynthesis with the insertion of pigment-binding proteins into the photosynthetic membrane by glueing the protein components catalyzing these functions into a supercomplex and regulating its activity.


Assuntos
Arabidopsis , Xantofilas , Adesivos/metabolismo , Animais , Arabidopsis/metabolismo , Clorofila/metabolismo , Humanos , Fotossíntese , Tilacoides/metabolismo , Xantofilas/metabolismo , Zeaxantinas/metabolismo
16.
Int J Mol Sci ; 22(2)2021 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-33445775

RESUMO

Accelerating wound healing with minimized bacterial infection has become a topic of interest in the development of the new generation of tissue bio-adhesives. In this study, we fabricated a hydrogel system (MGC-g-CD-ic-TCS) consisting of triclosan (TCS)-complexed beta-cyclodextrin (ß-CD)-conjugated methacrylated glycol chitosan (MGC) as an antibacterial tissue adhesive. Proton nuclear magnetic resonance (1H NMR) and differential scanning calorimetry (DSC) results showed the inclusion complex formation between MGC-g-CD and TCS. The increase of storage modulus (G') of MGC-g-CD-ic-TCS after visible light irradiation for 200 s indicated its hydrogelation. The swollen hydrogel in aqueous solution resulted in two release behaviors of an initial burst and sustained release. Importantly, in vitro and in vivo results indicated that MGC-g-CD-ic-TCS inhibited bacterial infection and improved wound healing, suggesting its high potential application as an antibacterial tissue bio-adhesive.


Assuntos
Adesivos/química , Quitosana/química , Glicóis/química , Hidrogéis/química , Triclosan/química , beta-Ciclodextrinas/química , Animais , Antibacterianos/química , Antibacterianos/farmacologia , Luz , Masculino , Camundongos , Ratos , Ratos Sprague-Dawley , Cicatrização/efeitos dos fármacos
17.
Dent Mater J ; 40(2): 538-546, 2021 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-33328395

RESUMO

This study evaluated the effects of remaining dentin thickness (RDT), different smear layers, and aging on the microtensile bond strength (µTBS) of universal adhesives to dentin when applied in self-etch mode. Ninety-six human third molars were randomly allocated to 12 groups (n=8) based on adhesives: ClearfilTM SE Bond 2 (SE, control), ClearfilTM Universal Bond (CU) and ScotchBondTM Universal Adhesive (SB); smear layers: prepared either with 600-grit SiC paper (P) or regular diamond bur (B); and aging: stored in distilled water at 37ºC for 24 hours (24h) or 1 year (1y). µTBS was significantly affected by the type of adhesives, smear layers, and aging (p<0.001). A statistically significant and positive linear relationship was also observed between µTBS and RDT (p<0.05) in all the tested groups, except for SEB1y and CUB24h (p>0.05). RDT, smear layer types, and aging can influence the bonding performances of universal adhesives when applied in self-etch mode.


Assuntos
Colagem Dentária , Camada de Esfregaço , Adesivos , Envelhecimento , Cimentos Dentários , Dentina , Adesivos Dentinários , Humanos , Teste de Materiais , Cimentos de Resina , Resistência à Tração
18.
J Mater Chem B ; 9(3): 783-792, 2021 01 28.
Artigo em Inglês | MEDLINE | ID: mdl-33333547

RESUMO

Pesticides play a very important role in pest control and plant protection. However, they can be limited by a tendency to cause ecological system damage due to significant losses into the environment. To increase pesticide utilization efficiency, we developed highly leaf-adhesive avermectin nanocapsules (Av-pH-cat@CS) with pH-responsive controlled release properties. The Av-pH-cat@CS nanocapsules displayed good thermal stability and photostability in response to UV light irradiation. The Av-pH-cat@CS nanocapsules could be disrupted at low pH and they exhibited excellent controlled release in response to pH, which improved the release of avermectins. In addition, the Av-pH-cat@CS nanocapsules were highly adhesive to crop leaves as a result of strong hydrogen bonding, which prolonged the retention time on crop leaves. The Av-pH-cat@CS nanocapsules with pH-responsive release and strong leaf adhesion improved the control efficacy and enhanced the utilization efficiency. Our findings offer a promising approach to prolonging pesticide duration on crop leaves and improving the utilization efficiency.


Assuntos
Adesivos/química , Antiprotozoários/química , Ivermectina/análogos & derivados , Nanocápsulas/química , Praguicidas/química , Adesivos/síntese química , Antiprotozoários/síntese química , Liberação Controlada de Fármacos , Ligação de Hidrogênio , Concentração de Íons de Hidrogênio , Ivermectina/síntese química , Ivermectina/química , Estrutura Molecular , Tamanho da Partícula , Praguicidas/síntese química , Folhas de Planta/química , Propriedades de Superfície , Fatores de Tempo , Zea mays/química
19.
Methods Mol Biol ; 2211: 183-191, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33336278

RESUMO

The simple and versatile CRISPR/Cas9 system is a promising strategy for genome editing in mammalian cells. Generally, the genome editing components, namely Cas9 protein and single-guide RNA (sgRNA), are delivered in the format of plasmids, mRNA, or ribonucleoprotein (RNP) complexes. In particular, non-viral approaches are desirable as they overcome the safety concerns posed by viral vectors. To control cell fate for tissue regeneration, scaffold-based delivery of genome editing components will offer a route for local delivery and provide possible synergistic effects with other factors such as topographical cues that are co-delivered by the same scaffold. In this chapter, we detail a simple method of surface modification to functionalize electrospun nanofibers with CRISPR/Cas9 RNP complexes. The mussel-inspired bio-adhesive coating will be used as it is a simple and effective method to immobilize biomolecules on the surface. Nanofibers will provide a biomimicking microenvironment and topographical cues to seeded cells. For evaluation, a model cell line with single copies of enhanced green fluorescent protein (U2OS.EGFP) will be used to validate the efficiency of gene disruption.


Assuntos
Sistemas CRISPR-Cas , Edição de Genes , Ribonucleoproteínas/metabolismo , Tecidos Suporte , Adesivos/química , Animais , Proteína 9 Associada à CRISPR/química , Linhagem Celular , Expressão Gênica , Técnicas de Transferência de Genes , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Lipídeos/química , Substâncias Macromoleculares/química , Substâncias Macromoleculares/metabolismo , Nanofibras/química , RNA Guia/química , RNA Guia/genética , Ribonucleoproteínas/genética , Engenharia Tecidual , Tecidos Suporte/química
20.
Carbohydr Polym ; 251: 117019, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33142580

RESUMO

The advent of high-performance conductive organohydrogels, which are sustainable in extremely cold environment, has attracted immersing interest in biosensors. In this work, a highly stretchable, self-healable, adhesive and antibacterial cellulose-based ionic conductive organohydrogel with low-temperature strain sensitivity was developed, using in-situ polymerization of acrylamide in glycerol-water with poly (vinyl alcohol), chitosan, FeCl3 and 2,2,6,6-Tetramethylpiperidine-1-oxyl oxidized cellulose nanofibril (TCNF). Owing to their chemically cross-linked structures and multiple H-bonding networks, the organohydrogel exhibits excellent mechanical properties, such as high stretchability (540 %), high compression strength (0.44 MPa), nearly 87 % self-healing efficiency and adhesive to various substrates. Also, good antibacterial property was confirmed by the diameter of inhibition zone (∼5.1 mm) against Salmonella enteritidis. Notably, the organohydrogels remained high conductivity and flexibility even below -20 °C, which can be applied as low-temperature strain sensor for real-time. Therefore, it has promising applications in artificial intelligence and personal healthcare under cold environment.


Assuntos
Acrilamida/química , Antibacterianos/química , Técnicas Biossensoriais , Celulose/química , Quitosana/química , Hidrogéis , Adesivos/química , Antibacterianos/farmacologia , Temperatura Baixa , Condutividade Elétrica , Hidrogéis/síntese química , Hidrogéis/química , Fenômenos Mecânicos , Salmonella enteritidis/efeitos dos fármacos
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