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1.
Braz Oral Res ; 33: e034, 2019 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-31038568

RESUMO

Specific variants in genes that encode adipokines and their mRNA and protein expression were previously studied in type 2 diabetes mellitus (T2DM) and obesity, and similar studies have been performed for chronic periodontitis (CP). The aim of this case-control study was to investigate the possible impacts of adiponectin (ADIPOQ), leptin (LEP) and its receptor (LEPR), and resistin (RETN) on the etiopathogenesis of CP. Examinations were performed on 118 non-periodontitis healthy subjects (healthy controls, HC), 205 healthy individuals with CP (H + CP) and 86 type 2 diabetes patients with CP (T2DM + CP). Variants within the ADIPOQ (rs2241766, rs1501299), LEP (rs13228377, rs2167270), LEP receptor (rs1805096), and RETN (rs1862513) genes were determined by qPCR. In addition, the plasma levels of ADIPOQ, LEP, and RETN were analysed by ELISA for 80 individuals. The genotype frequencies of the SNP ADIPOQ +45G/T (rs2241766) differed between the HC and H + CP groups (p=0.03, pcorr>0.05), and carriers of the TT genotype had a lower risk of developing CP compared to carriers of the GG or TG genotypes (p<0.01, pcorr>0.05). However, there were no significant differences in the plasma levels of ADIPOQ, LEP or RETN between the study groups (p > 0.05). Plasma levels of the adipokines were also independent of the gene profiles (p > 0.05). Adipokine plasma levels did not change in patients with H + CP/T2DM + CP compared to HC, but we did identify a specific polymorphism in the ADIPOQ gene that was associated with CP. Although the ADIPOQ +45G/T (rs2241766) gene variant may be a candidate biomarker for CP, further research is required in larger populations with different ethnic backgrounds before any final conclusions can be drawn about the role of this gene in CP.


Assuntos
Adipocinas/sangue , Adipocinas/genética , Periodontite Crônica/sangue , Diabetes Mellitus Tipo 2/sangue , Polimorfismo de Nucleotídeo Único , Adulto , Idoso , Análise de Variância , Biomarcadores/sangue , Estudos de Casos e Controles , Periodontite Crônica/genética , Diabetes Mellitus Tipo 2/genética , Feminino , Variação Genética , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Valores de Referência , Estatísticas não Paramétricas
2.
Anticancer Res ; 39(4): 1739-1748, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30952713

RESUMO

BACKGROUND/AIM: Obesity is a risk factor for cancer. Disruption of the daily feeding and fasting rhythm can contribute to obesity. This study tested the hypothesis that time-restricted feeding (TRF) attenuates obesity-enhanced metastasis. MATERIALS AND METHODS: In a spontaneous metastasis model of Lewis lung carcinoma (LLC), male C57BL/6 mice were fed the standard AIN93G diet or a high-fat diet (HFD) with or without dark-phase restricted feeding (12 h per day) for 10 weeks. Pulmonary metastases from a subcutaneous tumor were quantified. RESULTS: The number and size of lung metastases were greater in the HFD group than in the AIN93G group, but did not differ between the TRF and AIN93G groups. TRF prevented HFD-induced increases in plasma concentrations of glucose, insulin, proinflammatory cytokines (leptin, monocyte chemotactic protein-1, plasminogen activator inhibitor-1), and angiogenic factors (angiopoietin-2, hepatic growth factor, vascular endothelial growth factor). CONCLUSION: TRF attenuates the HFD-enhanced spontaneous metastasis of LLC in mice.


Assuntos
Carcinoma Pulmonar de Lewis/prevenção & controle , Carcinoma Pulmonar de Lewis/secundário , Dieta Hiperlipídica/efeitos adversos , Jejum , Neoplasias Pulmonares/prevenção & controle , Neoplasias Pulmonares/secundário , Obesidade/prevenção & controle , Adipocinas/sangue , Adiposidade , Proteínas Angiogênicas/sangue , Animais , Glicemia/metabolismo , Carcinoma Pulmonar de Lewis/sangue , Carcinoma Pulmonar de Lewis/genética , Peptídeos e Proteínas de Sinalização do Ritmo Circadiano/genética , Peptídeos e Proteínas de Sinalização do Ritmo Circadiano/metabolismo , Citocinas/sangue , Mediadores da Inflamação/sangue , Insulina/sangue , Neoplasias Pulmonares/sangue , Neoplasias Pulmonares/genética , Masculino , Camundongos Endogâmicos C57BL , Obesidade/sangue , Obesidade/etiologia , Obesidade/genética , Fatores de Tempo , Ganho de Peso
3.
Saudi Med J ; 40(4): 353-359, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30957128

RESUMO

OBJECTIVES: To investigate the cord blood levels of adipokine and to assess their association with the fetal insulin resistance and fetal outcomes in newborns of gestational diabetic women (GDM). Methods: This cross-sectional study was performed in 40 GDM women and 40 healthy pregnant women (HPW) in the Department of Obstetrics and Gynecology at Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) hospital in Puducherry, India, during the period from May 2016 to December 2017. Cord blood samples were collected at delivery from GDM and HPW groups. Cord plasma biochemical parameters such as insulin, C-peptide, adiponectin, leptin, resistin, and visfatin concentrations were measured. Leptin/adiponectin ratio (L/A), homeostasis model assessment of insulin resistance (HOMA2-IR), insulin sensitivity (HOMA2-%S) and beta cell function (HOMA2-%B) were calculated. The pregnancy outcomes such as birth weight (BW), Ponderal index and Apgar scores of the baby were measured. Results: The BW and Ponderal index of the baby were found to be significantly higher in GDM newborns compared to HPW newborns. Cord plasma insulin, C-peptide, HOMA2 -IR, visfatin, leptin, and L/A ratio were significantly higher whereas adiponectin level was lower in GDM compared to HPW. A significant positive correlation was observed between L/A ratio and fetal HOMA2-IR. Conclusion: Altered adipokine levels with increased L/A ratio was observed among the new-borns of Indian gestational diabetic mothers. There was an association between increased L/A ratio, insulin resistance and increased Ponderal index among the new-borns.


Assuntos
Adipocinas/sangue , Diabetes Gestacional/sangue , Diabetes Gestacional/fisiopatologia , Sangue Fetal/metabolismo , Resistência à Insulina , Resultado da Gravidez , Índice de Apgar , Biomarcadores/sangue , Peso ao Nascer , Peptídeo C/sangue , Estudos Transversais , Diabetes Gestacional/diagnóstico , Feminino , Humanos , Índia , Recém-Nascido , Insulina/sangue , Leptina/sangue , Nicotinamida Fosforribosiltransferase/sangue , Gravidez , Resistina/sangue
4.
Food Chem Toxicol ; 129: 64-76, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31026535

RESUMO

Exposures to persistent environmental pollutants like polychlorinated biphenyls (PCBs) has been associated with liver diseases such as toxicant-associated steatohepatitis (TASH). However, previously published PCB hepatotoxicity studies evaluated mostly male animal models. Moreover, epidemiologic studies on PCB-exposed cohorts evaluating sex differences are scarce. Therefore, the objective of this study was to examine hepato-toxicological responses of PCB exposures in the context of sex-dependent outcomes. Male and female C57Bl/6 mice were exposed to Aroclor 1260 (20 mg/kg), and PCB126 (20 µg/kg), by gavage for two weeks. Female mice appeared to be more sensitive to PCB-induced hepatotoxic effects as manifested by increased liver injury markers, namely, hepatic Serpine1 expression. Additionally, compared to their male counterparts, PCB-exposed females exhibited dysregulated hepatic gene expression favoring lipid accumulation rather than lipid breakdown; accompanied by dyslipidemia. Sex differences were also observed in the expression and activation of PCB targets such as the epidermal growth factor receptor (EGFR) while PCB-induced pancreatic toxicity was similar in both sexes. Importantly, PCB exposure appeared to cause pro-androgenic, anti-estrogenic along with sex-dependent thyroid hormone effects. The overall findings demonstrated that the observed PCB-mediated hepatotoxicity was sex-dependent; confirming the existence of sex differences in environmental exposure-induced markers of TASH and warrants further investigation.


Assuntos
Arocloros/toxicidade , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Disruptores Endócrinos/toxicidade , Bifenilos Policlorados/toxicidade , Fatores Sexuais , Adipocinas/sangue , Animais , Peso Corporal/efeitos dos fármacos , Citocinas/sangue , Feminino , Glucose/metabolismo , Lipídeos/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Tamanho do Órgão/efeitos dos fármacos
5.
Med Sci Monit ; 25: 2337-2343, 2019 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-30928991

RESUMO

BACKGROUND Meteorin-like (Metrnl) is a novel adipomyokine that may improve glucose tolerance and affect insulin resistance. This study aimed to investigate the association between serum levels of Metrnl with blood glucose status and to its association with insulin resistance. MATERIAL AND METHODS The study included 160 subjects with normal glucose tolerance (NGT) (n=40), impaired fasting glucose (IFG) (n=40), impaired glucose tolerance (IGT) (n=40), and newly diagnosed type 2 diabetes mellitus (T2DM) (n=40). An enzyme-linked immunosorbent assay (ELISA) was used to measure the serum levels of Metrnl. Partial correlation analysis was used to analyze the relationship between serum levels of Metrnl and metabolic parameters. Multiple logistic regression analysis was performed to identify the association between serum levels of Metrnl with the risk of diabetes. RESULTS Serum levels of Metrnl was highest in patients with T2DM and significantly increased in patients with prediabetes compared with individuals with NGT. After adjusting for age, gender, and body mass index (BMI), serum Metrnl level was significantly correlated with lipid profile, glucose profile, and insulin resistance. Multiple logistic regression analysis showed that Metrnl significantly increased the risk of T2DM (OR=1.727; P=0.008) before adjusting for the homeostatic model assessment of insulin resistance (HOMA-IR). When further adjusted for HOMA-IR, Metrnl was no longer associated with an increased OR for T2DM (OR=1.491; P=0.066), while the HOMA-IR significantly increased the risk of T2DM (OR=1.935; P=0.008). CONCLUSIONS Serum levels of Metrnl were significantly increased in patients with T2DM and may increase the risk of T2DM independent of insulin resistance.


Assuntos
Diabetes Mellitus Tipo 2/metabolismo , Resistência à Insulina/fisiologia , Adipocinas/análise , Adipocinas/sangue , Adulto , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Índice de Massa Corporal , China , Diabetes Mellitus Tipo 2/sangue , Feminino , Glucose/metabolismo , Intolerância à Glucose/sangue , Teste de Tolerância a Glucose/métodos , Humanos , Insulina/sangue , Insulina/metabolismo , Masculino , Pessoa de Meia-Idade , Estado Pré-Diabético/sangue
6.
Int J Biol Macromol ; 132: 606-614, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-30904536

RESUMO

GABPB1, known as nuclear respiratory factor 2 (Nrf2), activates mitochondrial genes that are responsible for oxidative phosphorylation. Earlier studies on GABPB1 reported that two single nucleotide polymorphisms (SNPs) such as rs7181866 and rs8031031, to be associated with increased endurance in athletes. In the present study, a cohort of 302 South Indians, including normoglycemic healthy controls, T2DM with and without obesity were genotyped for the two SNPs by PCR-RFLP method and correlated with serum adipokines. The 'G' allele of rs7181866 was found to be associated with obesity whereas rs8031031 didn't show any significant association with obese individuals. The increased levels of adipokines such as Leptin, IL-6 and TNF-α and decreased adiponectin were found among obese-T2DM, when compared to non-obese T2DM subjects. Further, Factor analysis on metabolic components revealed four factors which accounts for 71.5% for non-obese control and 88.3% for obese T2DM of variance. The bias-corrected and accelerated bootstrap analysis revealed GG genotype to have significant positive and negative correlation with both TNF-α and adiponectin. In conclusion, the G allele of (rs7181866 A/G) was found to be significantly associated with risk for obesity among T2DM subjects.


Assuntos
Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/genética , Fator de Transcrição de Proteínas de Ligação GA/genética , Obesidade/complicações , Polimorfismo de Nucleotídeo Único , Adipocinas/sangue , Sequência de Bases , Biomarcadores/sangue , Estudos de Coortes , Diabetes Mellitus Tipo 2/sangue , Feminino , Predisposição Genética para Doença/genética , Humanos , Índia , Masculino , Pessoa de Meia-Idade
7.
Artigo em Inglês | MEDLINE | ID: mdl-30832279

RESUMO

Obesity is a chronic condition whose incidence is growing due to lack of exercise and frequent nutrition disorders. Childhood obesity has reached epidemic proportions worldwide. One of the best treatment methods is physical training. However, conflicting results have been reported regarding its clinical effectiveness. These contrasting findings may be due to the type and intensity of the adopted physical training program. Therefore, the purpose of the current study was to investigate the effect of an 8-week individualized physical training program on endothelial function, blood biomarkers and adipokine levels in obese children with and without metabolic syndrome (MS). One-hundred-and-twenty-two obese adolescents (71 obese without MS and 51 obese with MS) aged 14 ± 2 years were included in this study. The 8-week individualized training program decreased glucose, triglycerides, total cholesterol, low-density lipoprotein cholesterol and leptin in obese subjects with and without MS. However, adiponectin and endothelial-dependent vasodilatation increased in the follow-up study in both groups. Taken together, the findings suggest that individualized training program is an effective means for the treatment of obesity and MS in pediatric populations.


Assuntos
Adipocinas/sangue , Obesidade Pediátrica , Adolescente , Biomarcadores/sangue , Índice de Massa Corporal , Criança , LDL-Colesterol , Exercício , Feminino , Seguimentos , Humanos , Leptina/sangue , Masculino , Síndrome Metabólica/epidemiologia , Triglicerídeos/sangue
8.
Nutr Hosp ; 36(1): 60-65, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30834760

RESUMO

Introduction: Background and objectives: the aim of the present investigation was to describe the association of this single nucleotide polymorphism (SNP) with fasting glucose levels, serum adipokine levels and diabetes mellitus. Methods: the study involved a population of 1,002 adult obese subjects. Measurements of anthropometric parameters, blood pressure, fasting blood glucose, C-reactive protein (CRP), insulin concentration, insulin resistance (HOMA-IR), HOMA-B, lipid profi le and adipocytokines levels were performed. Genotype of MTNR1B gene polymorphism (rs10830963) was evaluated. Results: fasting glucose levels (GG: 101.5 ± 19.1 mg/dl vs GT: 103.5 ± 8.1 units vs TT: 107.2 ± 8.0 mg/dl; p = 0.01) and HOMA-IR (GG: 3.1 ± 1.6 units vs GT: 3.4 ± 1.1 units vs TT: 3.7 ± 1.0 units; p = 0.02) were higher in subjects with GG genotype than in other genotypes. Total adiponectin levels (CC: 20.5 ± 8.4 ng/dl vs CG: 21.8 ± 5.4 ng/dl vs GG: 15.4 ± 1.4 ng/dl; p = 0.02) and HOMA-B (CC: 2.3 ± 0.8 units vs CG: 2.2 ± 1.1 units vs GG: 1.9 ± 0.9 units; p = 0.01) were lower in subjects with GG genotype than GC or CC genotypes. Logistic regression analysis showed an increased risk of hyperglicemia (OR = 1.31, 95% CI = 1.12-2.78, p = 0.03) and diabetes mellitus (OR = 1.37, 95% CI = 1.14-2.86, p = 0.04). Conclusions: this study showed that the MTNR1B rs10830963 polymorphism was associated with increased fasting glucose levels, HOMA-IR, and risk of DM2. This SNP was associated with decreased adiponectin levels and HOMA-B.


Assuntos
Adipocinas/sangue , Glicemia/análise , Síndrome Metabólica/sangue , Síndrome Metabólica/genética , Obesidade/sangue , Obesidade/genética , Polimorfismo Genético/genética , Receptor MT2 de Melatonina/genética , Adulto , Idoso , Antropometria , Pressão Sanguínea , Proteína C-Reativa/análise , Diabetes Mellitus Tipo 2/epidemiologia , Diabetes Mellitus Tipo 2/genética , Feminino , Genótipo , Humanos , Insulina/sangue , Resistência à Insulina , Lipídeos/sangue , Masculino , Pessoa de Meia-Idade
9.
Nutr Hosp ; 36(1): 80-86, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30836761

RESUMO

Introduction: Background: the aim of this study was to evaluate the intake of nutrients, anthropometric parameters, health indicators, adipokines and insulin levels in a population of young undergraduates. Method: in this study, 378 young undergraduates were invited to participate. Due to the inclusion criteria and their own decision of participating, 90 attended the anthropometric, health indicators: waist circumference (WC), waist to hip ratio (WHR), waist to height ratio (WHtR), and homeostatic model assessment-insulin resistance index (HOMA-IR) studies and completed the questionnaire of frequency of food intake; and 34 participants were selected to perform the determination of biochemical parameters, insulin and adipokines levels: leptin, IL-6, IL-8, tumor necrosis factor alpha (TNF-α), monocyte chemoattractant protein-1 (MCP-1) and hepatocyte growth factor (HGF). Results: according to WC, WHR and WHtR, obese population showed health, cardiovascular and metabolic risk. Overweight population showed cardiometabolic risk. In general, lipid intake was higher than 30%, being animal fat the most consumed. The levels of leptin (women: 17.2 ± 9.2, 28 ± 11.3, 36.8 ± 17.8; men: 4.3 ± 3.6, 9.5 ± 3.1, 24.6 ± 16.4 to lean overweight and obese, respectively) and insulin (women: 408 ± 182, 438 ± 187, 768 ± 167; men: 244 ± 88, 520 ± 256, 853 ± 590) increased along with body mass index (BMI), body fat percentage (BFP), visceral fat area (VFA), WC, WHR and WHtR. Lean (2.4 ± 1.3), overweight (2.2 ± 0.9) and obese (4.3 ± 1.1) women and overweight (2.8 ± 1.2) and obese (5.0 ± 3.1) men showed insulin resistance according to HOMA-IR. Significant correlation between leptin and HOMA-IR was found (p = 0.41). BMI, BFP, VFA, WC, and WHtR positively correlated with leptin (p = 0.67, 0.75, 0.66, 0.60, 0.67, respectively) and insulin (p = 0.37, 0.40, 0.48, 0.49, 0.42, respectively), while WHR only with insulin (p = 0.43). No significant differences were found in the other adipokines. Conclusion: the use of health indicators such VFA, WC, WHR, WHtR and HOMA-IR are useful tools in the determination of health, cardio vascular and metabolic risk and are correlated with levels of leptin and insulin in the studied population.


Assuntos
Adipocinas/sangue , Antropometria , Dieta , Adiposidade , Composição Corporal , Índice de Massa Corporal , Estudos Transversais , Comportamento Alimentar , Feminino , Humanos , Resistência à Insulina , Leptina/sangue , Masculino , Obesidade/sangue , Sobrepeso/sangue , Fatores Sexuais , Estudantes , Circunferência da Cintura , Relação Cintura-Quadril , Adulto Jovem
10.
Diabetes Res Clin Pract ; 150: 57-63, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30825562

RESUMO

OBJECTIVE: Meteorin-like (Metrnl) is a novel secreted protein that has a beneficial effect on glucose homeostasis with anti-inflammatory properties. Our goal is to determine whether low serum Metrnl levels are associated with worsening of glucose tolerance, impaired endothelial function, and atherosclerosis. METHODS: This study included 260 adults, 89 of whom had normal oral glucose tolerance (nOGT), 77 with glucose tolerance impairment (GTI) and 94 with type 2 diabetes (T2DM). Insulin resistance was assessed by evaluating the homeostasis model assessment of insulin resistance (HOMA-IR). Serum Metrnl level, proinflammatory, biochemical, endothelial and atherosclerosis parameters were measured. RESULTS: Serum Metrnl levels decreased significantly in patients with T2DM versus subjects with nOGT (P < 0.001). Metrnl levels were negatively correlated with fasting blood glucose, 2-h postload glucose (2 h-PLG), fasting insulin, HOMA-IR, HbA1c, high-sensitive C-reactive protein (hs-CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), Carotid intima media thickness (CIMT), brachial-ankle pulse wave velocity (baPWV), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and E-selectin. High serum Metrnl level was significantly correlated with reduced risk of T2DM as revealed by multivariate logistic regression analysis after control of potential risk factors for diabetes. Furthermore, the association remains significant after further adjustment for IL-6, TNF-α, hs-CRP, CIMT, baPWV, ICAM-1, VCAM-1 and E-selectin. CONCLUSIONS: Low Serum Metrnl may be associated with worsening of glucose tolerance, impaired endothelial function and atherosclerosis. It may also be considered a possible surrogate marker of endothelial dysfunction, and atherosclerosis and an independent risk factor of T2DM.


Assuntos
Adipocinas/sangue , Aterosclerose/sangue , Biomarcadores/sangue , Diabetes Mellitus Tipo 2/sangue , Endotélio Vascular/metabolismo , Intolerância à Glucose/sangue , Resistência à Insulina , Adulto , Idoso , Índice Tornozelo-Braço , Aterosclerose/epidemiologia , Glicemia/análise , Proteína C-Reativa/metabolismo , Espessura Intima-Media Carotídea , Estudos Transversais , Diabetes Mellitus Tipo 2/epidemiologia , Selectina E/sangue , Egito/epidemiologia , Endotélio Vascular/patologia , Feminino , Intolerância à Glucose/epidemiologia , Humanos , Molécula 1 de Adesão Intercelular/sangue , Interleucina-6/sangue , Masculino , Pessoa de Meia-Idade , Análise de Onda de Pulso , Fatores de Risco , Fator de Necrose Tumoral alfa/metabolismo , Molécula 1 de Adesão de Célula Vascular/sangue , Adulto Jovem
11.
Lipids Health Dis ; 18(1): 73, 2019 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-30917813

RESUMO

Fetuin-A (FetA), which impairs insulin action is considered classically as a hepatokine. In patients with Metabolic Syndrome without the confounding of diabetes or cardiovascular diseases, we showed significant increases in both circulating and subcutaneous adipose tissue secreted Fet-A. Furthermore we showed in mice models increase mRNA and protein following a high fat diet and in a model of metabolic syndrome. This work was recently confirmed by another group of investigators. Hence we propose that Fet-A be considered also as an adipokine.


Assuntos
Diabetes Mellitus/metabolismo , Síndrome Metabólica/metabolismo , Obesidade/metabolismo , alfa-2-Glicoproteína-HS/metabolismo , Adipócitos/metabolismo , Adipocinas/sangue , Adipocinas/metabolismo , Adiponectina/metabolismo , Diabetes Mellitus/sangue , Diabetes Mellitus/genética , Diabetes Mellitus/patologia , Feminino , Humanos , Resistência à Insulina/genética , Masculino , Síndrome Metabólica/sangue , Síndrome Metabólica/genética , Síndrome Metabólica/patologia , Obesidade/sangue , Obesidade/genética , Obesidade/patologia , Gordura Subcutânea/metabolismo
12.
Lipids ; 54(4): 203-210, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30883766

RESUMO

VASPIN, visceral adipose tissue-derived serpin, is an adipokine ameliorating insulin resistance in obesity. Here, we investigated the role of VASPIN and its genetic variants in lipid metabolism. We measured serum VASPIN concentrations by ELISA in 823 metabolically well-characterized Caucasian subjects (Sorbs from Germany). Furthermore, we genotyped 30 representative single nucleotide polymorphisms (SNP) in two independent cohorts with metabolic phenotyping, the Sorbs (N = 823) and Leipzig (N = 919), and conducted genotype-phenotype association analyses. Circulating VASPIN strongly correlated with triacylglycerol levels (TAG; p = 1.079 × 10-11 ), and moderately with apolipoprotein A1 and low-density lipoprotein cholesterol (p = 0.026). Genetic variants in VASPIN were nominally associated with cholesterol, high-density and low-density lipoprotein (HDL-chol, LDL-chol), lipoprotein A, and apolipoprotein B as well as with TAG and free fatty acids (all p < 0.05 adjusted for age, sex, and body mass index [BMI]). Mendelian randomization analysis using VASPIN SNP as an instrumental variable showed borderline influence of VASPIN on LDL-chol levels (p = 0.05). Associations of VASPIN and its genetic variation with metabolic traits suggest a role of VASPIN in human lipid metabolism.


Assuntos
Adipocinas/sangue , Serpinas/sangue , Adulto , Apolipoproteína A-I/sangue , Apolipoproteínas B/sangue , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Metabolismo dos Lipídeos/fisiologia , Lipoproteína(a)/sangue , Masculino , Pessoa de Meia-Idade , Obesidade/sangue , Triglicerídeos/sangue
13.
Nutrients ; 11(3)2019 Mar 04.
Artigo em Inglês | MEDLINE | ID: mdl-30836628

RESUMO

(1) Background: High iron associates with inflammation and type 1 diabetes (T1D). Iron is essential not only for neonatal development but also for infectious microorganisms. The neonatal immune system is immature, and innate immunity prevails before immunocompetence develops. (2) Methods: In 398 newborns from the Danish Newborn Screening Biobank, we examined if whole blood iron (WB-Iron) content were associated with cytokines, adipokines, C-reactive protein (CRP), and mannose-binding lectin (MBL) in non-infected healthy neonates, and if these associations differed in newborns who later developed T1D (cases) (n = 199). WB-Iron was quantified using laser ablation inductively coupled plasma mass spectrometry on the neonatal dried blood spots. For each analyte, the relative change (RC) in the mean level was modeled by robust log-normal regression. (3) Results: A one unit increase in neonatal WB-Iron was associated with a 38% decrease in mean interleukin (IL)-6 levels (0.62; 95% CI: 0.40⁻0.95, p = 0.03), and a 37% decrease in mean MBL levels (0.63; 95% CI: 0.41⁻0.95, p = 0.03), but was not statistically significant after correction for multiple testing. (4) Conclusions: In summary, we found that higher neonatal WB-iron content was inversely associated with IL-6 and MBL, which may increase susceptibility to infections.


Assuntos
Adipocinas/sangue , Citocinas/sangue , Doenças do Recém-Nascido/imunologia , Ferro/sangue , Lectina de Ligação a Manose/sangue , Adipocinas/imunologia , Bancos de Espécimes Biológicos , Biomarcadores/sangue , Proteína C-Reativa/análise , Proteína C-Reativa/imunologia , Citocinas/imunologia , Dinamarca , Diabetes Mellitus Tipo 1/sangue , Feminino , Voluntários Saudáveis , Humanos , Imunidade Inata/imunologia , Recém-Nascido , Doenças do Recém-Nascido/sangue , Interleucina-6/sangue , Interleucina-6/imunologia , Ferro/imunologia , Masculino , Lectina de Ligação a Manose/imunologia , Análise de Regressão
14.
Environ Pollut ; 247: 1055-1063, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30823334

RESUMO

Exposures to perfluoroalkyl substances (PFAS) including perfluoroalkyl acids (PFAAs) are associated with increased liver enzymes in cohort studies including the C8 Health Study. In animal models, PFAAs disrupt hepatic lipid metabolism and induce apoptosis to cause nonalcoholic fatty liver disease (NAFLD). PFAAs are immunotoxic and inhibit pro-inflammatory cytokine release from stimulated leukocytes in vitro. This cross-sectional study tests the hypothesis that environmental PFAAs are associated with increased hepatocyte apoptosis and decreased pro-inflammatory cytokines in serum. Biomarkers previously associated with PFAS exposures and/or NAFLD were evaluated as secondary endpoints. Two hundred adult C8 Health Study participants were included. Measured serum biomarkers included: perfluorohexane sulfonate (PFHxS); perfluorooctanoic acid (PFOA); perfluorooctane sulfonate (PFOS); perfluorononanoic acid (PFNA); cytokeratin 18 M30 (CK18 M30, hepatocyte apoptosis); adipocytokines; insulin; and cleaved complement 3 (C3a). Confounder-adjusted linear regression models determined associations between PFAS and disease biomarkers with cut-offs determined by classification and regression tree analysis. CK18 M30 was positively associated with PFHxS (ß = 0.889, p = 0.042); PFOA (ß = 2.1, p = 0.005); and PFNA (ß = 0.567, p = 0.03). Tumor necrosis factor α (TNFα) was inversely associated with PFHxS (ß = -0.799, p = 0.001); PFOA (ß = - 1.242, p = 0.001); and PFOS (ß = -0.704, p < 0.001). Interleukin 8 was inversely associated with PFOS and PFNA. PFAAs were also associated with sexually dimorphic adipocytokine and C3a responses. Overall, PFAA exposures were associated with the novel combination of increased biomarkers of hepatocyte apoptosis and decreased serum TNFα. These data support previous findings from cohorts and experimental systems that PFAAs may cause liver injury while downregulated some aspects of the immune response. Further studies of PFAAs in NAFLD are warranted and should evaluate sex differences.


Assuntos
Adipocinas/sangue , Apoptose/efeitos dos fármacos , Biomarcadores/sangue , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Exposição Ambiental/efeitos adversos , Fluorcarbonetos/efeitos adversos , Metabolismo dos Lipídeos/efeitos dos fármacos , Adulto , Idoso , Animais , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
15.
World J Gastroenterol ; 25(9): 1116-1131, 2019 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-30862999

RESUMO

BACKGROUND: Non-alcoholic steatohepatitis (NASH) has become one of the leading causes of liver disease in the western world. In obese patients weight reduction is recommended. Up to now there are no specific guidelines for weight loss in order to reduce hepatic fat content. AIM: To investigate the effects of a 24-wk guided lifestyle intervention program compared to a meal replacement regimen based on soy protein. METHODS: Twenty-six subjects with NASH participated in a randomized single-center study. They were randomly assigned to either meal replacement group (MR-G) with soy-yogurt-honey preparation or to guided lifestyle change group (LC-G) with endurance activity and nutrition counselling. Serum alanine transaminase (ALT), aspartate transaminase (AST), lipid parameters, and adipokines were measured. Liver fat content and lipid composition were determined by magnetic resonance imaging and magnetic resonance spectroscopy. Body fat mass and lean body mass were assessed using Bod Pod® device. Pre- and post-intervention monitoring of parameters was performed. Statistical analyses were conducted with SPSS software, results were expressed as median (interquartile range). RESULTS: Twenty-two subjects (MR-G, n = 11 and LC-G, n = 11) completed the study (9 women, 13 men; age 52.1 (15.0) years, body mass index (BMI) 32.3 (3.3) kg/m²). In both groups a significant weight loss was achieved (MR-G: -6.4 (3.6) kg, P < 0.01; LC-G: -9.1 (10.4) kg, P < 0.01). BMI dropped in both groups (MR-G: -2.3 (1.5) kg/m2, P = 0.003; LC-G: -3.0 (3.4) kg/m2, P = 0.006). Internal fat and hepatic lipid content were markedly reduced in both groups in comparable amount. There was a strong correlation between reduction in liver fat and decrease in ALT. Likewise, both groups showed an improvement in glycemic control and lipid profile. Changes in adipokines, particularly in adiponectin and leptin were closely related to intrahepatic lipid changes. CONCLUSION: Comprehensive lifestyle intervention and meal replacement regimen have comparable effects on body and liver fat, as well as decrease in markers of hepatic inflammation among NASH patients.


Assuntos
Terapia Comportamental/métodos , Dieta Redutora/métodos , Estilo de Vida Saudável , Hepatopatia Gordurosa não Alcoólica/terapia , Obesidade/terapia , Proteínas de Vegetais Comestíveis/administração & dosagem , Proteínas de Soja/administração & dosagem , Adipocinas/sangue , Adulto , Idoso , Restrição Calórica , Feminino , Humanos , Fígado/diagnóstico por imagem , Imagem por Ressonância Magnética , Masculino , Refeições , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/sangue , Hepatopatia Gordurosa não Alcoólica/diagnóstico por imagem , Hepatopatia Gordurosa não Alcoólica/etiologia , Obesidade/sangue , Obesidade/complicações , Obesidade/diagnóstico por imagem , Resultado do Tratamento , Perda de Peso
16.
Turk J Med Sci ; 49(1): 238-244, 2019 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-30761880

RESUMO

Background/aim: Recent data draw attention to the effect of body composition, insulin resistance, and adipocytokines to acne vulgaris (AV) development. The aim of this study was to assess the association of AV with insulin resistance and adipocytokine levels and to evaluate the effect of isotretinoin on insulin resistance and adipocytokine levels. Materials and methods: In 29 AV patients and 29 healthy volunteers, body mass index (BMI) and body fat mass (BFM), lipid, adiponectin, leptin, resistin, retinol binding protein-4 (RBP4), and insulin levels were measured and insulin resistance was assessed by HOMA-IR index in serum samples taken twice from patients before and after isotretinoin treatment. Results: In AV patients, pretreatment HOMA-IR and adipocytokine levels were not found to correlate with disease severity. With five months of isotretinoin treatment, higher HOMA-IR values were found (P = 0.028). Isotretinoin therapy maintained lower mean resistin levels (P = 0.016), higher mean RBP4 levels (P = 0.040), but not affected the mean adiponectin and leptin levels (P = 0.113, P = 0.125, respectively). Conclusions: All data suggests that five months of isotretinoin therapy in AV patients causes insulin resistance and the increase in in-sulin resistance is not dependent on age, BMI, BFM, and lipid levels of these patients.


Assuntos
Acne Vulgar/tratamento farmacológico , Adipocinas/sangue , Resistência à Insulina , Isotretinoína/efeitos adversos , Isotretinoína/uso terapêutico , Adolescente , Adulto , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Adulto Jovem
17.
PLoS One ; 14(1): e0209911, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30689664

RESUMO

BACKGROUND: Comparison of changes in body composition, adipokines and inflammatory markers after initial therapy with a nucleos(t)ide reverse transcriptase inhibitor (N(t)RTI)- sparing or containing regimen are scarce. DESIGN: Randomised Clinical Trial. METHODS: This is the body composition substudy of NEAT 001/ANRS 143, a randomised trial comparing darunavir/ritonavir (DRV/r) plus either raltegravir (RAL) or tenofovir disoproxil fumarate/emtricitabine (TDF/FTC) in 805 ART naïve HIV-infected adults. The primary endpoint was percentage change in limb fat at week 96. Secondary endpoints were associations among these changes and metabolic markers (IL-6, insulin, leptin, adiponectin, FGF-23). RESULTS: 126 subjects (61 DRV/r + RAL and 65 DRV/r + TDF/FTC) were included. The rate of change in BMI between groups for RAL versus TDF/FTC at week 96 was 1.5% per 48-week period (p = 0.015). The rate of change in limb fat mass, trunk fat mass, total body fat and total lean mass was for RAL versus TDF/FTC at week 96 was 2.5% (p = 0.38), 7.3% ((p = 0.021), 4.9% (p = 0.061) and 1.3% (p = 0.12) respectively. Baseline insulin and leptin levels were correlated with baseline limb fat and trunk fat mass [r = 0.31 (p = 0.0043)/r = 0.28 (p = 0.0011) for limb fat, and r = 0.63 (p<0.0001)/r = 0.50(p<0.0001) for trunk fat]. After adjustment, a 10% faster increase in leptin between baseline and week 48 was associated with a more rapid increase in limb fat at week 48 (0.5% per 48 weeks, p<0.001), total body fat mass (0.6% per 48 weeks, p<0.001), and trunk fat mass (0.3% per 48 weeks, p = 0.0026). CONCLUSIONS: After week 96 a N(t)RTI sparing regimen of DRV/r + RAL produced a numerically greater percentage increase in body composition variables with only change in trunk fat mass and BMI being significant.


Assuntos
Adipocinas/sangue , Composição Corporal , Índice de Massa Corporal , Combinação Emtricitabina e Fumarato de Tenofovir Desoproxila/administração & dosagem , Raltegravir Potássico/administração & dosagem , Adulto , Combinação Emtricitabina e Fumarato de Tenofovir Desoproxila/efeitos adversos , Feminino , Humanos , Masculino , Raltegravir Potássico/efeitos adversos , Fatores de Tempo
18.
Diabetes Metab Syndr ; 13(1): 530-534, 2019 Jan - Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30641760

RESUMO

AIMS: Adipokines play a major role in developing metabolic syndrome (MetS), and it has been found that there is a significant relationship between MetS and resting metabolic rate (RMR) in obese people. The present study aimed to investigate the mediatory effect of adipokines on the RMR-MetS relationship. METHODS: This cross-sectional study included 263 obese and overweight women, mean BMI 33.28 (4.93) kg/m, and mean age 39.02 (11.60) who were assessed for RMR using indirect calorimetry. Moreover, using the body composition analyzer the Body composition was measured. Also, Enzyme-linked Immunosorbent Assay (ELISA) test provided a quantitative measurement of biochemical parameters. RESULTS: The results indicated that women in low RMR group had higher fat mass (P < 0.0001), FFM (P = 0.002), weight (P = 0.006), BMI (P < 0.0001), age (P = 0.01), and hs-CRP (P = 0.001). The results did not confirm any significant mediating roles for RBP4 (P = 0.051, ß = -0.28) and Vaspin (P = 0.06, ß = 0.32) in the RMR-MetS relationship. Additionally, after a binary regression test, Omentin-1 showed a significant mediating role (P = 0.25, ß = 0.04) as an interrelated agent to RMR and MetS. CONCLUSION: As this study shows, Omentin-1 was found to play a significant mediating role as a mediatory agent in relationship between RMR and MetS.


Assuntos
Adipocinas/sangue , Metabolismo Basal/fisiologia , Síndrome Metabólica/sangue , Síndrome Metabólica/diagnóstico , Sobrepeso/sangue , Sobrepeso/diagnóstico , Adolescente , Adulto , Idoso , Biomarcadores/sangue , Estudos Transversais , Feminino , Humanos , Síndrome Metabólica/epidemiologia , Pessoa de Meia-Idade , Obesidade/sangue , Obesidade/diagnóstico , Obesidade/epidemiologia , Sobrepeso/epidemiologia , Adulto Jovem
19.
Diabetes Metab Syndr ; 13(1): 874-880, 2019 Jan - Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30641824

RESUMO

AIMS: The current study aimed to explore the correlation between leukocytes and platelets indices with adipocytokines (leptin and adiponectin) and MetS components. METHODS: A total of 100 healthy subjects and 200 patients diagnosed with different MetS components were enrolled in the study. Eligible patients were allocated into four groups (50 patients). Group1 include patients with 2 criteria of MetS components, group 2 with 3 criteria, group 3 with 4 criteria and group 4 had patients with 5 criteria. RESULTS: Regarding white blood cell indices, data showed that total leukocyte and neutrophil count as well as neutrophil/lymphocyte (N/L) ratio were significantly increased in all groups of MetS patients when compared to the healthy group. Additionally, platelets count, platelet distribution width (PDW), and main platelet volume (MPV) levels and platelets/lymphocyte (P/L) ratio were significantly higher in all patients with MetS as compared to the healthy subjects. Serum leptin concentration and leptin-to-adiponectin ratio (LAR) were elevated significantly, while adiponectin level was significantly diminished in all MetS groups when compared to the control. CONCLUSION: leukocytes and platelets indices were associated with hyperleptinemia and hypoadiponectinemia as well as MetS components. The study also suggested the necessary role of leukocytes, platelet indices, and LAR as markers in early diagnoses of individuals with MetS components.


Assuntos
Adipocinas/sangue , Plaquetas/metabolismo , Leucócitos/metabolismo , Síndrome Metabólica/sangue , Síndrome Metabólica/diagnóstico , Adulto , Glicemia/metabolismo , Feminino , Humanos , Masculino , Volume Plaquetário Médio/métodos , Pessoa de Meia-Idade
20.
Diabetes Metab Syndr ; 13(1): 24-29, 2019 Jan - Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30641704

RESUMO

Obesity is the main determinant of type 2 diabetes. Some adipocytokines play important roles in diabetic complications. Lipid transport is an important aspect of lipid metabolism in cancer. Present study aimed to evaluate the effect of some adipocytokines, inflammatory, epigenetic instability & angiogenesis biomarkers in type 2 diabetic Egyptian women with breast cancer. Study Design was performed on eighty females divided into 20 healthy subjects (Group I), 20 patients with type 2 diabetes (Group II), 20 patients with breast cancer (Group III) & 20 patients with diabetes and breast cancer (Group IV). Demographic data & body mass index have been collected. Biochemical analysis included fasting & postprandial blood glucose, lipid profile, fatty acid-binding proteins-4 (FABP-4), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), 8-hydroxy-2'-deoxyguanosine (8-OHdG) & thioredoxin reductase (TrxR) activity. Results revealed significant increase in FABP-4, TNF-α, VEGF, 8-OHdG and significant decreased TrxR activity in diabetic patients with breast cancer in comparison with other groups. These changes were evident in breast cancer subjects than diabetic and healthy cases and in diabetic than healthy cases. Conclusion: This study confirmed the role of FABP-4 in pathogenesis of type 2 diabetes & breast cancer via enhancing angiogenesis, inflammatory and epigenetic instability biomarkers.


Assuntos
Adipocinas/sangue , Moduladores da Angiogênese/metabolismo , Biomarcadores/análise , Neoplasias da Mama/etiologia , Diabetes Mellitus Tipo 2/complicações , Epigênese Genética , Mediadores da Inflamação/sangue , Neoplasias da Mama/sangue , Neoplasias da Mama/patologia , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/genética , Egito , Proteínas de Ligação a Ácido Graxo/sangue , Feminino , Seguimentos , Instabilidade Genômica , Humanos , Pessoa de Meia-Idade , Obesidade/sangue , Obesidade/complicações , Obesidade/patologia , Prognóstico , Fator A de Crescimento do Endotélio Vascular/sangue
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