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1.
PLoS One ; 15(1): e0225662, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31978107

RESUMO

BACKGROUND: Obesity is a major risk factor for many chronic diseases, including reduced lung function. The role of polymorphisms of the adiponectin gene, though linked with cardiometabolic consequences of obesity, has not been studied in relation to lung function. OBJECTIVES: The aim of this study is to examine polymorphisms in the ADIPOQ, ADIPOR1, and ADIPOR2 genes in relation to adiponectin serum levels, BMI, and adiposity in 18-year old Cypriot males, as well as determine whether BMI, adipokines levels and polymorphisms in adipokine related genes are associated with lung function levels. RESULTS: From the participants, 8% were classified as obese, 22% as overweight, and the remaining 71% as normal. We found that rs266729 and rs1501299 in ADIPOQ and rs10920531 in ADIPOR1 were significantly associated with serum adiponectin levels, after adjusting for ever smoking. In addition, there was an overall significant increase in FEV1% predicted with increasing BMI (ß = 0.53, 95% CI: 0.27, 0.78) and in FVC % predicted (ß = 1.02, 95% CI: 0.73, 1.30). There was also a decrease in FEV1/FVC with increasing BMI (ß = -0.53, 95% CI: -0.71, -0.35). Finally, rs1501299 was associated with lung function measures. DISCUSSION: Functional variants in the ADIPOQ gene were linked with lung function in young males. Further studies should concentrate on the role of adipokines on lung function which may direct novel therapeutic approaches.


Assuntos
Adiponectina/sangue , Adiponectina/genética , Pulmão/fisiologia , Polimorfismo de Nucleotídeo Único , Adiposidade/genética , Adolescente , Índice de Massa Corporal , Humanos , Masculino , Obesidade/sangue , Obesidade/fisiopatologia , Receptores de Adiponectina/genética , Adulto Jovem
2.
Metabolism ; 102: 154008, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31706980

RESUMO

Recent scientific efforts have focused on the detrimental effects that obesity has on the metabolic function of skeletal muscles and whether exercise can improve this dysfunction. In this regard, adiponectin, with important metabolic functions (e.g. insulin-sensitizer and anti-inflammatory), has been recently described as a myokine that acts in an autocrine/paracrine manner. Earlier studies reported that muscle adiponectin could be induced by pro-inflammatory mediators (e.g. lipopolysaccharide), cytokines, and high-fat diets, providing a protective mechanism of this tissue against metabolic insults. However, when metabolic insults such as high-fat diets are sustained this protective response becomes dysregulated, making the skeletal muscle susceptible to metabolic impairments. Recent studies have suggested that exercise could prevent or even reverse this process. Considering that most scientific knowledge on adiponectin dysregulation in obesity is from the study of adipose tissue, the present review summarizes and discusses the literature available to date regarding the effects of obesity on skeletal muscle adiponectin induction, along with the potential effects of different exercise prescriptions on this response in an obesity context.


Assuntos
Adiponectina/metabolismo , Exercício Físico/fisiologia , Músculo Esquelético/metabolismo , Obesidade/metabolismo , Adiponectina/genética , Animais , Humanos , Resistência à Insulina/fisiologia , Músculo Esquelético/fisiologia , Obesidade/genética , Regulação para Cima/genética
3.
Gene ; 730: 144302, 2020 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-31884106

RESUMO

OBJECTIVES: This study aimed to investigate the roles of adiponectin (ADIPOQ) rs266729 and melatonin receptor 1B (MTNR1B) rs10830963 polymorphisms in patients with gestational diabetes mellitus (GDM). METHODS: The databases including Web of science, PubMed, Embase and Cochrane Library were searched for case-control studies updating on Feb 15th, 2019. 12 studies were finally included containing 3759 GDM patients and 4422 controls. The associations between two polymorphic sites (rs266729 and rs10830963) and GDM susceptibility were analyzed according to the pooled odds ratios (OR) and corresponding 95% confidence intervals (CI). RESULTS: Our pooled results indicated that ADIPOQ rs266729 can increase the GDM risk in Asian (dominant: GG+CG vs. CC, OR = 2.079, 95%CI: 1.012-4.270, P = 0.046) and European (allele: G vs. C, OR = 1.353, 95%CI: 1.001-1.829, P = 0.049 dominant: GG+CG vs. CC, OR = 1.522, 95%CI: 1.031-2.245, P = 0.034 heterozygote: CG vs. CC, OR = 1.517, 95%CI: 1.006-2.287, P = 0.047), but decrease in American population (allele: G vs. C, OR = 0.663, 95%CI: 0.501-0.878, P = 0.004 (dominant: GG+CG vs. CC, OR = 0.642, 95%CI: 0.456-0.903, P = 0.011 recessive: GG vs. CC+CG, OR = 0.496, 95%CI: 0.250-0.981, P = 0.044). The data demonstrated that the correlation between MTNR1B rs10830963 and GDM susceptibility is significant in Asian (allele: G vs. C, OR = 1.236, 95%CI: 1.109-1.378, P < 0.001 dominant: GG+CG vs. CC, OR = 1.285, 95%CI: 1.135-1.455, P < 0.001 recessive: GG vs. CC+CG, OR = 1.884, 95%CI: 1.307-2.716, P = 0.001 homozygote: GG vs. CC, OR = 1.514, 95%CI: 1.231-1.864, P < 0.001 heterozygote: CG vs. CC, OR = 1.198, 95%CI: 1.050-1.367, P = 0.007), and European (heterozygote: CG vs. CC, OR = 1.302, 95%CI: 1.011-1.677, P = 0.041). CONCLUSIONS: This Meta-analysis suggests that the variation of ADIPOQ rs266729 can increase the risk of GDM in Asian and European, while reduce in American population. Additionally, the association between MTNR1B rs10830963 and GDM susceptibility is significant in Asian, European and when using TaqMan assay.


Assuntos
Adiponectina/genética , Diabetes Gestacional/genética , Receptor MT2 de Melatonina/genética , Adiponectina/metabolismo , Alelos , Estudos de Casos e Controles , Grupos de Populações Continentais/genética , Feminino , Frequência do Gene/genética , Predisposição Genética para Doença/genética , Humanos , Razão de Chances , Polimorfismo de Nucleotídeo Único/genética , Gravidez , Receptor MT2 de Melatonina/metabolismo , Fatores de Risco
4.
Cell Biochem Funct ; 37(8): 625-632, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31693217

RESUMO

The fat-derived protein adiponectin is known to reverse the effects of insulin resistance and to lower blood glucose levels. The AMP-activated protein kinase (AMPK) signalling pathway plays a central role in metabolism and energy homeostasis. Here, to investigate the role of AMPK in the protective effect of adiponectin against insulin resistance, we established the model of high-glucose (HG)- and high-lipid (HL)-induced insulin resistance in INS-1 pancreatic ß cells. We found that 25mM of glucose and 0.4mM of palmitic acid treatment significantly increased cell apoptosis and impaired insulin secretion in INS-1 cells. However, recombinant human adiponectin dramatically reduced HG- and/or HL-induced cell apoptosis and greatly improved insulin secretion. Interestingly, adiponectin treatment also activated AMPK signalling pathway by increasing the phosphorylation of Thr172 in the AMPK α subunit; 10µM of compound C, a potent AMPK inhibitor, blocked the protective effects of adiponectin against HG/HL-induced insulin resistance. Furthermore, knockout experiments by CRISPR/Cas9 technology showed that AMPK α1, but not AMPK α2, is involved in the protective effects of adiponectin. Taken together, adiponectin reversed the effects of insulin resistance via AMPK α1, which provides a novel insight into the protective mechanism of adiponectin and may be used as a new strategy for the treatment of type 2 diabetes. SIGNIFICANCE OF THE STUDY: Adiponectin can reverse the effects of insulin resistance and lower blood glucose levels. Here, adiponectin reduced HG/HL-induced cell apoptosis and greatly improved insulin secretion. These effects were blocked by AMPK inhibitor, compound C. Specifically, we found that AMPK α1, but not AMPK α2, mediates the protective effects of adiponectin, which provides a novel insight into the protective mechanism of adiponectin against insulin resistance.


Assuntos
Proteínas Quinases Ativadas por AMP/metabolismo , Adiponectina/farmacologia , Proteínas Quinases Ativadas por AMP/antagonistas & inibidores , Proteínas Quinases Ativadas por AMP/genética , Adiponectina/genética , Adiponectina/metabolismo , Animais , Apoptose/efeitos dos fármacos , Linhagem Celular , Glucose/farmacologia , Resistência à Insulina , Células Secretoras de Insulina/citologia , Células Secretoras de Insulina/metabolismo , Fosforilação/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Ratos , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/farmacologia , Transdução de Sinais/efeitos dos fármacos
5.
J Biosci ; 44(4)2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31502564

RESUMO

Vascular calcification is a common problem in the elderly with diabetes, heart failure and end-stage renal disease. The differentiation of vascular smooth muscle cells (VSMCs) into osteoblasts is the main feature, but the exact mechanism remains unclear. It is not clear whether adiponectin (APN) affects osteogenic differentiation of VSMCs. This study aims to explore the effect of APN on vascular calcification by using a cell model induced by beta-glycerophosphate (beta-GP). VSMCs were isolated and treated with beta-GP and APN in this study. The alkaline phosphatase (ALP) activity and expression levels of Runx2, BMP-2, collagen type I and osteocalcin were determined. The expression levels of STAT3 and p-STAT3 in nucleus and cytoplasm of VSMCs were analyzed. The results showed that APN significantly inhibited the expression of ALP, Runx2, BMP-2, collagen I, osteocalcin and the formation of the mineralized matrix in VSMCs induced by beta-GP. APN reduces the osteogenic differentiation of VSMCs induced by beta-GP and down-regulates the expression of the osteogenic transcription factor osterix by inhibiting STATS3 phosphorylation and nuclear transport. APN may be one of the potential candidates for clinical treatment of vascular calcification.


Assuntos
Adiponectina/genética , Osteogênese/genética , Fator de Transcrição STAT3/genética , Calcificação Vascular/genética , Animais , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Glicerofosfatos/farmacologia , Humanos , Janus Quinase 2/antagonistas & inibidores , Janus Quinase 2/genética , Camundongos , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/metabolismo , Miócitos de Músculo Liso/efeitos dos fármacos , Miócitos de Músculo Liso/metabolismo , Osteogênese/efeitos dos fármacos , RNA/genética , Fator de Transcrição STAT3/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos , Fator de Transcrição Sp7/genética , Calcificação Vascular/induzido quimicamente , Calcificação Vascular/patologia
6.
Anim Reprod Sci ; 208: 106110, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31405469

RESUMO

Adiponectin is an adipocyte derived cytokine implicated in energy homeostasis, insulin resistance and is involved in the regulation of reproduction both centrally and peripherally in animals. The present study was conducted to investigate adiponectin (ADIPOQ) and its receptors ADIPOR1 and ADIPOR2 abundance of mRNA transcript and protein in different stages of corpora lutea (CL) development during the estrous cycle of water buffalo and to determine the effect of adiponectin on cultured luteal cells of water buffalo (Bubalus bubalis). The results indicate adiponectin, ADIPOR1, and ADIPOR2 were present in buffalo corpora lutea (CL) throughout the estrous cycle. The abundance of adiponectin and its receptors was greater in the early and regressing and was less in mid- and late-stages of CL functionality. Adiponectin and its receptors were localized in the cytoplasm of small and large luteal cells. Furthermore, luteal cells were cultured in the in-vitro culture system and were treated with 1 and 10 µg/mL dose of adiponectin for 48 h. Adiponectin at both doses decreased (P < 0.05) progesterone (P4) secretion from cultured luteal cells and also suppressed the abundance of factors involved in P4productionv [Steroidogenic Acute Regulatory Protein (STAR), cytochrome P45011A1 (CYP11A1) and 3ß-hydroxysteroid dehydrogenase (HSD3B1) at the 10 µg/mL dose as compared to adiponectin non-supplemented cells]. In conclusion, results of the present study indicate adiponectin and its receptors are present in bubaline CL and adiponectin inhibits P4 production in cultured luteal cells. The findings indicate adiponectin affects luteal dynamics and reproductive functions in water buffalo.


Assuntos
Adiponectina/metabolismo , Búfalos/fisiologia , Corpo Lúteo/metabolismo , Ciclo Estral/fisiologia , Regulação da Expressão Gênica/fisiologia , RNA Mensageiro/metabolismo , Adiponectina/genética , Animais , Enzima de Clivagem da Cadeia Lateral do Colesterol/genética , Enzima de Clivagem da Cadeia Lateral do Colesterol/metabolismo , Feminino , Imuno-Histoquímica , Proteínas de Membrana Transportadoras/genética , Proteínas de Membrana Transportadoras/metabolismo , Complexos Multienzimáticos/genética , Complexos Multienzimáticos/metabolismo , Progesterona/metabolismo , Progesterona Redutase/genética , Progesterona Redutase/metabolismo , RNA Mensageiro/genética , Receptores de Adiponectina/genética , Receptores de Adiponectina/metabolismo , Esteroide Isomerases/genética , Esteroide Isomerases/metabolismo
7.
Endocrinology ; 160(10): 2367-2387, 2019 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-31265057

RESUMO

Prolonged exposure to glucocorticoids (GCs) causes various metabolic derangements. These include obesity and insulin resistance, as inhibiting glucose utilization in adipose tissues is a major function of GCs. Although adipose tissue distribution and glucose homeostasis are sex-dependently regulated, it has not been evaluated whether GCs affect glucose metabolism and adipose tissue functions in a sex-dependent manner. In this study, high-dose corticosterone (rodent GC) treatment in C57BL/6J mice resulted in nonfasting hyperglycemia in male mice only, whereas both sexes displayed hyperinsulinemia with normal fasting glucose levels, indicative of insulin resistance. Metabolic testing using stable isotope-labeled glucose techniques revealed a sex-specific corticosterone-driven glucose intolerance. Corticosterone treatment increased adipose tissue mass in both sexes, which was reflected by elevated serum leptin levels. However, female mice showed more metabolically protective adaptations of adipose tissues than did male mice, demonstrated by higher serum total and high-molecular-weight adiponectin levels, more hyperplastic morphological changes, and a stronger increase in mRNA expression of adipogenic differentiation markers. Subsequently, in vitro studies in 3T3-L1 (white) and T37i (brown) adipocytes suggest that the increased leptin and adiponectin levels were mainly driven by the elevated insulin levels. In summary, this study demonstrates that GC-induced insulin resistance is more severe in male mice than in female mice, which can be partially explained by a sex-dependent adaptation of adipose tissues.


Assuntos
Glicemia/metabolismo , Corticosterona/toxicidade , Resistência à Insulina , Proteínas Proto-Oncogênicas c-akt/metabolismo , Adipocinas/genética , Adipocinas/metabolismo , Adiponectina/genética , Adiponectina/metabolismo , Tecido Adiposo/efeitos dos fármacos , Animais , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Glucose/metabolismo , Homeostase/efeitos dos fármacos , Insulina/farmacologia , Leptina/genética , Leptina/metabolismo , Masculino , Camundongos , Fatores Sexuais
8.
Biomed Khim ; 65(3): 239-244, 2019 Apr.
Artigo em Russo | MEDLINE | ID: mdl-31258148

RESUMO

The purpose of the study was to investigate the features of expression and adiponectin content in the adipocyte culture of subcutaneous, epicardial, and perivascular adipose tissue and the effect of various doses of rosuvastatin on these processes. 29 patients with coronary artery disease were examined. Adipocytes were isolated from the samples of SAT, EAT and PVAT which were taken during coronary artery bypass surgery, followed by cultivation in the presence of rosuvastatin and evaluation of gene expression and adiponectin concentration. Adipocytes SAT, EAT and PVAT differed in the level of adiponectin secretion and expression of its gene. On day 1 of cultivation the expression of the adiponectin gene in the EAT was 2.3 times lower than in the PVAT. On day 2 of cultivation the expression of the adiponectin gene was reduced both in the EAT and the PVAT as compared to the SAT. When rosuvastatin was added at a concentration of 1 mmol/L, adiponectin gene expression in PVAT was higher than when rosuvastatin was added at a concentration of 5 mmol/L, in the adipocyte culture of SAT effect was opposite. Thus, the adipocytes of EZhT and, to a greater extent, PAS, can be a therapeutic target for statins in the case of the pathological activation of adipose tissue.


Assuntos
Adiponectina/metabolismo , Tecido Adiposo/metabolismo , Doença da Artéria Coronariana/metabolismo , Adipócitos/efeitos dos fármacos , Adiponectina/genética , Células Cultivadas , Doença da Artéria Coronariana/genética , Expressão Gênica , Humanos , Rosuvastatina Cálcica/farmacologia
9.
Reprod Domest Anim ; 54(9): 1291-1303, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31339602

RESUMO

Adiponectin is an adipocyte-derived hormone regulating energy metabolism, insulin sensitivity and recently found to regulate reproduction. The current study was carried out to investigate gene and protein expression, immunolocalization of adiponectin and its receptors AdipoR1 and AdipoR2 in ovarian follicles of different developmental stages in water buffalo (Bubalus bubalis) and to investigate the effect of adiponectin on steroid production in cultured bubaline granulosa cells. qPCR, western blotting and immunohistochemistry were applied to demonstrate mRNA expression, protein expression and immunolocalization, respectively. The results indicate that adiponectin, AdipoR1 and AdipoR2 were present in granulosa cells (GC) and theca interna (TI) of ovarian follicles and the expression of adiponectin, AdipoR1, AdipoR2 in GC and AdipoR1 and AdipoR2 in TI increased with increase in follicle size (p < .05). Expression of adiponectin was high in small and medium size follicles in TI. The adiponectin and its receptors were immunolocalized in the cytoplasm of GC and TI cells. Further, in the in-vitro study, GCs were cultured and treated with recombinant adiponectin each at 0, 1 and 10 µg/ml alone or with follicle stimulating hormone (FSH) at 30 ng/ml) or Insulin-like growth factor I (IGF-I) at 10 ng/ml for 48 hr after obtaining 75%-80%s confluency. Adiponectin at 10 µg/ml increased IGF-I-induced estradiol (E2 ) and progesterone (P4 ) secretion and FSH-induced E2 secretion from GC and also increased the abundance of factors involved in E2 and P4 production (cytochrome P45019A1 [CYP19A1] and 3-beta-hydroxysteroid dehydrogenase [3ß-HSD]). In conclusion, this study provides novel evidence for the presence of adiponectin and its receptors in ovarian follicles and modulatory role of adiponectin on steroid production in buffalo.


Assuntos
Adiponectina/fisiologia , Búfalos/metabolismo , Receptores de Adiponectina/fisiologia , Adiponectina/genética , Adiponectina/farmacologia , Animais , Células Cultivadas , Estradiol/metabolismo , Feminino , Hormônio Foliculoestimulante/farmacologia , Regulação da Expressão Gênica , Células da Granulosa/metabolismo , Fator de Crescimento Insulin-Like I/farmacologia , Folículo Ovariano/metabolismo , Progesterona/metabolismo , Receptores de Adiponectina/genética , Células Tecais/metabolismo
10.
Int J Mol Sci ; 20(12)2019 Jun 13.
Artigo em Inglês | MEDLINE | ID: mdl-31200595

RESUMO

Adiponectin, the most abundant secreted adipokine, has received great attention from the scientific community since its discovery [...].


Assuntos
Adiponectina/metabolismo , Doenças Metabólicas/etiologia , Adiponectina/genética , Animais , Humanos , Transdução de Sinais
11.
Food Funct ; 10(6): 3696-3705, 2019 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-31168538

RESUMO

The consumption of diets rich in fat and refined sugars is recognized to be one of the causes of lifestyle disorders, and dietary fibres are being advocated to ameliorate the complications associated with these disorders. In the present study, the effects of two soluble fermentable fibres, viz., gum acacia and inulin on the progression of adiposity, insulin resistance, and the expression of genes related to metabolism were examined in C57BL/6 mice fed a high-fat and sucrose diet for 18 weeks. The feeding of either type of fibre resulted in decrease in body weight, epididymal fat mass, adipocyte size, hyperlipidaemia, hyperglycaemia and hyperinsulinemia. In the fibre-fed groups, the expressions of adiponectin and glucose transporter 4 in the epididymal fat increased significantly, while the expressions of leptin, interleukin 6 and tumor necrosis factor alpha decreased significantly. Moreover, the expressions of genes related to beta-oxidation, viz., carnitine palmitoyltransferase 1, peroxisome proliferator-activated receptor gamma co-activator 1ß, and peroxisome proliferator-activated receptor alpha in the liver tissue of the fibre-fed groups enhanced significantly. Furthermore, due to the feeding of either type of fibre, the expressions of zonula occludens 1 and fasting-induced adipose factor in the distal ileum and proglucagon in the colon increased significantly. From the results of the present study, it can be concluded that the beneficial effects of the fibres are mediated due to enhanced energy expenditure, improved intestinal integrity, and reduced inflammation.


Assuntos
Adiposidade , Goma Arábica/metabolismo , Resistência à Insulina , Inulina/metabolismo , Obesidade/tratamento farmacológico , Adiponectina/genética , Adiponectina/metabolismo , Animais , Carnitina O-Palmitoiltransferase/genética , Carnitina O-Palmitoiltransferase/metabolismo , Dieta Hiperlipídica/efeitos adversos , Sacarose na Dieta/efeitos adversos , Transportador de Glucose Tipo 4/genética , Transportador de Glucose Tipo 4/metabolismo , Humanos , Insulina/metabolismo , Leptina/genética , Leptina/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Obesidade/genética , Obesidade/metabolismo , Obesidade/fisiopatologia
12.
Int J Mol Sci ; 20(12)2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-31212761

RESUMO

Adipose tissue has been recognized as a complex organ with endocrine and metabolic roles. The excess of fat mass, as occurs during overweight and obesity states, alters the regulation of adipose tissue, contributing to the development of obesity-related disorders. In this regard, many epidemiological studies shown an association between obesity and numerous types of malignancies, comprising those linked to the endocrine system (e.g., breast, endometrial, ovarian, thyroid and prostate cancers). Multiple factors may contribute to this phenomenon, such as hyperinsulinemia, dyslipidemia, oxidative stress, inflammation, abnormal adipokines secretion and metabolism. Among adipokines, growing interest has been placed in recent years on adiponectin (APN) and on its role in carcinogenesis. APN is secreted by adipose tissue and exerts both anti-inflammatory and anti-proliferative actions. It has been demonstrated that APN is drastically decreased in obese individuals and that it can play a crucial role in tumor growth. Although literature data on the impact of APN on carcinogenesis are sometimes conflicting, the most accredited hypothesis is that it has a protective action, preventing cancer development and progression. The aim of the present review is to summarize the currently available evidence on the involvement of APN and its signaling in the etiology of cancer, focusing on endocrine malignancies.


Assuntos
Adiponectina/metabolismo , Tecido Adiposo/metabolismo , Neoplasias das Glândulas Endócrinas/etiologia , Obesidade/complicações , Obesidade/metabolismo , Adiponectina/química , Adiponectina/genética , Animais , Biomarcadores , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/metabolismo , Neoplasias das Glândulas Endócrinas/diagnóstico , Neoplasias das Glândulas Endócrinas/metabolismo , Neoplasias das Glândulas Endócrinas/terapia , Humanos , Insulina/metabolismo , Modelos Biológicos , Metástase Neoplásica , Comunicação Parácrina , Ligação Proteica , Receptores de Adiponectina/genética , Receptores de Adiponectina/metabolismo , Risco , Medição de Risco , Relação Estrutura-Atividade
13.
Biomarkers ; 24(6): 607-614, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31215811

RESUMO

Context: Metabolic imbalance in renal cell carcinoma (RCC) can lead to abnormal adiponectin levels. Objective: To evaluate circulating adiponectin as a detection or predictive marker for RCC. Methods: A comprehensive literature search and meta-analysis was performed on studies reporting circulating adiponectin levels and RCC. The meta-analysis was performed using RevMan. Results: Seven studies compared the circulating adiponection levels between RCC cases and controls. Adiponectin level was significantly lower in RCC cases compared to controls at pre-diagnosis and pre-operative time-points. RCC stage, grade and subtype did not affect adiponectin levels. Conclusion: Low circulating adiponectin could be a predictive or risk factor for RCC.


Assuntos
Adiponectina/genética , Biomarcadores Tumorais/genética , Carcinoma de Células Renais/diagnóstico , Neoplasias Renais/diagnóstico , Adiponectina/sangue , Biomarcadores Tumorais/sangue , Carcinoma de Células Renais/sangue , Carcinoma de Células Renais/genética , Carcinoma de Células Renais/patologia , Estudos de Casos e Controles , Expressão Gênica , Humanos , Neoplasias Renais/sangue , Neoplasias Renais/genética , Neoplasias Renais/patologia , Gradação de Tumores , Estadiamento de Neoplasias , Fatores de Risco
14.
J Pediatr Endocrinol Metab ; 32(7): 749-758, 2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31216264

RESUMO

Background The objective of this study was to investigate the association of polymorphisms in four genes, tumor necrosis factor-α (TNFA), patatin-like phospholipase domain containing 3 (PNPLA3), adiponectin (ADIPOQ) and apolipoprotein C3 (APOC3), with obesity and non-alcoholic fatty liver disease (NAFLD) in Asian Indian adolescents. Methods In this case-control study, 218 Asian Indian adolescents with overweight/obesity and 86 lean healthy adults without fatty liver were enrolled. Hepatic steatosis was assessed and graded by ultrasonography (USG). Serum insulin, lipids, alanine aminotransferase (ALT), aspartate aminotransferase (AST), TNF-α, adiponectin and apolipoprotein C3 were measured and genotyping was done. Frequencies of variant and wild genotypes in all adolescents and in the subgroups without steatosis, with grade 1 steatosis and with grade 2 or 3 steatosis were compared to those in the controls. The frequencies were also compared in the overweight adolescents with grade 2 or 3 steatosis and without steatosis. Results Variant genotypes of polymorphisms -863 C > A and -1031 T > C of the TNFA gene, 455 T > C of the APOC3 gene and the wild type of +276 G > T of the ADIPOQ gene were associated with obesity with odds ratios (OR, 95% confidence interval [CI]) of 2.5 (1.5-4.4), 2.5 (1.5-4.2), 2.0 (1.1-3.6) and 2.5 (1.4-5.0), respectively. Polymorphisms 455 T > C of APOC3 and rs738409 C > G of PNPLA3 were associated with NAFLD. Fasting insulin and triglycerides (TG) were higher in the adolescents with homozygous variant polymorphisms -1031 T > C of TNFA and 455 T > C of APOC3 genes, respectively. Conclusions Several polymorphisms were noted to have a significant association with obesity and NAFLD in Asian Indian adolescents.


Assuntos
Biomarcadores/análise , Hepatopatia Gordurosa não Alcoólica/genética , Obesidade Pediátrica/genética , Polimorfismo de Nucleotídeo Único , Adiponectina/genética , Adolescente , Adulto , Apolipoproteína C-III/genética , Estudos de Casos e Controles , Criança , Feminino , Seguimentos , Humanos , Lipase/genética , Masculino , Proteínas de Membrana/genética , Pessoa de Meia-Idade , Hepatopatia Gordurosa não Alcoólica/complicações , Hepatopatia Gordurosa não Alcoólica/patologia , Obesidade Pediátrica/complicações , Obesidade Pediátrica/patologia , Prognóstico , Fator de Necrose Tumoral alfa/genética
15.
PLoS Genet ; 15(6): e1008244, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31233501

RESUMO

Berardinelli-Seip congenital generalized lipodystrophy is associated with increased bone mass suggesting that fat tissue regulates the skeleton. Because there is little mechanistic information regarding this issue, we generated "fat-free" (FF) mice completely lacking visible visceral, subcutaneous and brown fat. Due to robust osteoblastic activity, trabecular and cortical bone volume is markedly enhanced in these animals. FF mice, like Berardinelli-Seip patients, are diabetic but normalization of glucose tolerance and significant reduction in circulating insulin fails to alter their skeletal phenotype. Importantly, the skeletal phenotype of FF mice is completely rescued by transplantation of adipocyte precursors or white or brown fat depots, indicating that adipocyte derived products regulate bone mass. Confirming such is the case, transplantation of fat derived from adiponectin and leptin double knockout mice, unlike that obtained from their WT counterparts, fails to normalize FF bone. These observations suggest a paucity of leptin and adiponectin may contribute to the increased bone mass of Berardinelli-Seip patients.


Assuntos
Adiponectina/genética , Leptina/genética , Lipodistrofia Generalizada Congênita/genética , Osteosclerose/genética , Adipócitos/metabolismo , Tecido Adiposo Marrom/metabolismo , Animais , Densidade Óssea/genética , Modelos Animais de Doenças , Feminino , Glucose/genética , Glucose/metabolismo , Humanos , Insulina/genética , Gordura Intra-Abdominal/metabolismo , Lipodistrofia Generalizada Congênita/complicações , Lipodistrofia Generalizada Congênita/patologia , Camundongos , Camundongos Knockout , Osteosclerose/etiologia , Osteosclerose/metabolismo , Osteosclerose/patologia , Esqueleto/metabolismo , Esqueleto/patologia , Gordura Subcutânea/metabolismo
16.
Braz J Med Biol Res ; 52(6): e8344, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31141089

RESUMO

Type 2 diabetes mellitus (T2D) is a common endocrine and metabolic disorder, and poses threats to human health worldwide. Recently, microRNAs (miRNAs) have been suggested to play important roles in the pathophysiology of T2D. In this study, we explored the role of miR-3666 in T2D. miR-3666 was significantly down-regulated in the serum of T2D patients when compared to that of healthy volunteers, and miR-3666 expression level was negatively correlated with blood glucose levels of T2D patients. Overexpression of miR-3666 inhibited cell proliferation, reduced insulin secretion, and promoted cell apoptosis of pancreatic ß-cell line (INS-1 cells). On the other hand, knockdown of miR-3666 had the opposite effects in INS-1 cells. The bio-informatics analysis using TargetScan revealed that adiponectin (ADIPOQ) was a downstream target of miR-3666, and the interaction between miR-3666 and ADIPOQ was validated by luciferase reporter assay. In addition, miR-3666 negatively regulated the mRNA and protein expression of ADIPOQ. Overexpression of ADIPOQ promoted insulin secretion after glucose stimulation, promoted cell proliferation, inhibited cell apoptosis, and partially abolished the effects of miR-3666 overexpression on insulin secretion, cell proliferation, and cell apoptosis of INS-1 cells. In conclusion, our results revealed that miR-3666 inhibited pancreatic cell proliferation, reduced insulin sensitivity, and promoted apoptosis by targeting ADIPOQ.


Assuntos
Diabetes Mellitus Tipo 2/fisiopatologia , Resistência à Insulina/fisiologia , Células Secretoras de Insulina/fisiologia , MicroRNAs/metabolismo , Adiponectina/genética , Adiponectina/metabolismo , Apoptose , Proliferação de Células , Diabetes Mellitus Tipo 2/metabolismo , Feminino , Citometria de Fluxo , Humanos , Células Secretoras de Insulina/metabolismo , Masculino , MicroRNAs/genética , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase em Tempo Real
17.
Probl Endokrinol (Mosk) ; 65(1): 31-38, 2019 05 14.
Artigo em Russo | MEDLINE | ID: mdl-31091048

RESUMO

Type 2 diabetes mellitus (T2DM) is one of the most acute problems of the modern world. The disease is characterized by high ratio of micro- and macrovascular complications. T2DM is a multifactorial and polygenic disease, structure of hereditary predisposition to which may be population-specific. Aim - the analysis of allelic associations of adiponectin gene (ADIPOQ, rs17366743) with T2DM, its clinical and metabolic characteristics and complications in T2DM patients resident in the Republic of Bashkortostan. MATERIAL AND METHODS: 3 PCR-based method of genotyping with polymorphic marker rs17366743 of ADIPOQ gene in 433 T2DM patients and 428 healthy controls, residents of Bashkortostan. RESULTS: The ratio of genotype CT and allele С was higher in T2DM patients compared with controls (15.7% vs. 6.8%; p=0.0002 and 7.8% vs. 3.4%; p<0.0001, respectively). Genotype ТТ and allele Т were less frequent in T2DM than in healthy subjects (84.3 and 93,2%; p=0.0002; 92.2 and 96.6%, p<0.0001, respectively). The association with the development of diabetic retinopathy and cataract was shown (p=0,044, p=0,008, respectively). CONCLUSIONS: Allele C and genotype CT are risk markers of T2DM (OR=2.43 and 2.56 respectively).


Assuntos
Adiponectina/genética , Diabetes Mellitus Tipo 2/genética , Polimorfismo de Nucleotídeo Único , Adulto , Alelos , Bashkiria , Feminino , Humanos , Masculino
18.
Acta Diabetol ; 56(10): 1121-1131, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31076892

RESUMO

AIMS: Adiponectin, visfatin, and omentin have been shown to be associated with insulin sensitivity and might have a role in the pathophysiology of gestational diabetes mellitus (GDM). This study aimed to (1) compare adiponectin, visfatin, and omentin mRNA expressions in placenta and their serum levels between normal pregnancy (NP) and GDM class A1 (GDMA1) pregnancy and (2) determine correlations between placental gene expressions as well as serum levels with maternal and neonatal clinical parameters in all, NP, and GDM subjects. METHODS: NP subjects (n = 37), who had normal medical history during their pregnancies without diagnosis of any abnormalities and GDMA1 subjects (n = 37), who were diagnosed since they had antenatal care, were recruited when they were in labor with a gestational age of at least 34 weeks. Clinical parameters and serum adiponectin, visfatin, and omentin levels were measured in the delivery room. RESULTS: GDMA1 subjects had higher serum visfatin and plasma glucose levels, but lower serum omentin levels (p  < 0.05 all) compared to controls, with comparable levels of placental adiponectin, visfatin, and omentin expressions, plasma insulin, and indices of insulin sensitivity and insulin resistance. Serum visfatin was negatively correlated with neonatal weight and length in the GDM group (p  < 0.05 all). Serum omentin was negatively correlated with pre-pregnancy body mass index and waist circumference only in the NP group (p  < 0.05 all). Serum adiponectin was negatively correlated with maternal age and HOMA-IR in the NP group (p  < 0.05 all) and with placental weight and serum omentin in the GDM group (p  < 0.05 all). CONCLUSIONS: In conclusion, in GDMA1, increased serum visfatin, which has insulin-mimetic effect, might be associated with a compensatory mechanism that improves the impaired insulin function. Decreased serum omentin in GDMA1, which is normally found in visceral obesity, might lead to insulin resistance and contribute to the pathophysiology of GDM.


Assuntos
Adiponectina , Citocinas , Diabetes Gestacional/sangue , Diabetes Gestacional/genética , Lectinas , Nicotinamida Fosforribosiltransferase , Placenta/metabolismo , Adiponectina/sangue , Adiponectina/genética , Adulto , Índice de Massa Corporal , Estudos de Casos e Controles , Citocinas/sangue , Citocinas/genética , Diabetes Gestacional/patologia , Feminino , Proteínas Ligadas por GPI/sangue , Proteínas Ligadas por GPI/genética , Expressão Gênica , Humanos , Insulina/sangue , Resistência à Insulina/fisiologia , Lectinas/sangue , Lectinas/genética , Nicotinamida Fosforribosiltransferase/sangue , Nicotinamida Fosforribosiltransferase/genética , Placenta/patologia , Gravidez
19.
Biosci Biotechnol Biochem ; 83(9): 1774-1781, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31130066

RESUMO

Previous studies including ours have shown that a low-protein diet up-regulates insulin signaling in the liver and muscle and induces fatty liver in rats. Adiponectin is known as an insulin-sensitizing adipocytokine. We, therefore, examined the effect of a low-protein diet on the adiponectin levels in rats. The low-protein diet significantly increased serum adiponectin level. However, mRNA and protein levels of adiponectin in white adipose tissue (WAT) were not changed by the low-protein diet. Since it is known that oligomerization is important to control serum adiponectin level, we examined the population of adiponectin oligomeric forms in WAT and found that low-protein diet did not change it. Despite these events, the amount of its secretion was significantly increased in the adipocytes isolated from WAT of low-protein diet-fed rats. These results indicate that a low-protein diet enhances adiponectin secretion, which is not due to the increased intracellular amount and oligomerization of adiponectin.


Assuntos
Adiponectina/metabolismo , Proteínas na Dieta/administração & dosagem , Adiponectina/genética , Tecido Adiposo Branco/citologia , Tecido Adiposo Branco/metabolismo , Animais , Biopolímeros/metabolismo , Resistência à Insulina , Masculino , RNA Mensageiro/genética , Ratos , Ratos Wistar
20.
Taiwan J Obstet Gynecol ; 58(3): 409-416, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31122534

RESUMO

OBJECTIVE: The etiology of polycystic ovarian syndrome (PCOS) has not yet been fully explained. Several studies suggested an association between two single nucleotide polymorphisms (T45G and G276T) of the ADIPOQ gene that encodes for the hormone adiponectin and PCOS susceptibility. Hence, we performed a meta-analysis to investigate the relationship of the two further. MATERIALS AND METHODS: Literature search was conducted in PubMed up to June 22, 2018, for related publications written in English. Selected data were extracted from the included studies and was subjected to analysis using Review Manager 5.3. Odds ratios (ORs) and 95% confidence intervals (CIs) were computed and pooled from the resulting studies. Subgroup analysis by ethnicity was also performed. RESULTS: Overall analysis showed that women with the G276T polymorphism have reduced susceptibility to PCOS (OR: 0.68; 95% CI: 0.60-0.78; PA < 0.001). While no significant association was observed for the T45G polymorphism (OR: 1.07; 95% CI: 0.93-1.24; PA = 0.34). Subgroup analysis, on the other hand, showed significant associations among East Asians (OR: 0.69; 95% CI: 0.57-0.82; PA < 0.001) for the G276T association. CONCLUSION: Results of this meta-analysis suggests that women with the G276T polymorphism are less likely to develop PCOS. However, more studies are needed to confirm the claims of this meta-analysis.


Assuntos
Adiponectina/genética , Síndrome do Ovário Policístico/genética , Adiponectina/metabolismo , Grupo com Ancestrais do Continente Asiático , Feminino , Estudos de Associação Genética , Humanos , Síndrome do Ovário Policístico/metabolismo , Polimorfismo de Nucleotídeo Único , Medição de Risco
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