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1.
Talanta ; 233: 122494, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34215112

RESUMO

Titanium dioxide nanoparticles (TiO2 NPs) are widely used in industry as a white pigment (paints, paper industry and toothpastes), photocatalysts (environmental decontamination and photovoltaic cells), inorganic UV filter (sunscreens and personal care products) and as a food additive (E171) and antimicrobial food packaging material. Silver nanoparticles (Ag NPs) are used in photonics, microelectronics, catalysis and medicine due to their catalytic activity, magnetic and optical polarizability, electrical and thermal conductivities and enhanced Raman scattering. They also have antibacterial, antifungal and antiviral activities, as well as anti-inflammatory potential. The huge increase in the use of nano-based products, mainly metallic NPs, implies the presence of nanomaterials in the environment, and hence, the unintentional human ingestion through water or foods (gastrointestinal tract is the main pathway of NPs intake in humans). The presence of TiO2 NPs and Ag NPs in seafood samples was firstly established using an ultrasound assisted enzymatic hydrolysis procedure and sp-ICP-MS analysis. Several clams, cockles, mussels, razor clams, oysters and variegated scallops, which contain TiO2 NPs and Ag NPs, were subjected to an in vitro digestion process simulating human gastrointestinal digestion in the stomach and in the small and large intestine to determine the bioaccessibility of these NPs. Caco-2 cells were selected as model of human intestinal epithelium for transport studies because of the development of membrane transporters that are responsible for the uptake of chemicals. Parameters as transepithelial electrical resistance (TEER) and permeability of Lucifer Yellow were studied for establishing cell monolayer integrity. TiO2 NPs and Ag NPs transport as well as total Ti and Ag concentrations passing through the gastrointestinal epithelial barrier model (0-2 h) were assessed by sp-ICP-MS and ICP-MS in several molluscs.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Células CACO-2 , Trato Gastrointestinal , Humanos , Alimentos Marinhos , Prata , Titânio
2.
Talanta ; 233: 122503, 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34215119

RESUMO

Brevetoxins (BTX) are pharmacologically active, lipid soluble cyclic polyether neurotoxins that are known to cause a wide range of neurological symptoms in humans.Harvesting and consumption of infected molluscs provide an entry point for BTXs into, the food chain, causing long-term health effects on accumulation for individuals, commonly in people with a compromised immune system and existing allergies. This study is an acoustic assay that has been constructed using a 9 MHz AT-cut quartz crystal resonator modified by attaching a specific single-stranded DNA aptamer. The DNA oligo modifies its conformation to attach itself to the binding site of the incoming BTX molecule resulting in a change in frequency on the QCR. A small Δf value was observed for lower concentrations of BTX indicating a small change in mass deposited on the crystal surface, while the opposite was true for higher concentrations. Cross-species behavior was evaluated using samples of similar origin, molecular weight and a combination of two toxins. The LOD of the fabricated QCR is 220 nM which is lower than the maximum recommended residue limit in food samples. Fresh mussel samples were spiked with known concentrations of BTX to evaluate its sensitivity in a food matrix. No interaction with other compounds was observed. Overall, this sensor finds potential application in the food sector (fishing units) where mussels are tested and graded for allergens and toxins before reaching the customer.


Assuntos
Bivalves , Oxocinas , Animais , Bioensaio , Humanos , Toxinas Marinhas , Alimentos Marinhos , Frutos do Mar/análise
3.
Sensors (Basel) ; 21(13)2021 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-34209984

RESUMO

Iodine is a trace chemical element fundamental for a healthy human organism. Iodine deficiency affects about 2 billion people worldwide causing from mild to severe neurological impairment, especially in children. Nevertheless, an adequate nutritional intake is considered the best approach to prevent such disorders. Iodine is present in seawater and seafood, and its common forms in the diet are iodide and iodate; most iodide in seawater is caused by the biological reduction of the thermodynamically stable iodate species. On this basis, a multisensor instrument which is able to perform a multidimensional assessment, evaluating iodide content in seawater and seafood (via an electrochemical sensor) and discriminating when the seafood is fresh or defrosted quality (via a Quartz Micro balance (QMB)-based volatile and gas sensor), is strategic for seafood quality assurance. Moreover, an electronic interface has been opportunely designed and simulated for a low-power portable release of the device, which should be able to identify seafood over or under an iodide threshold previously selected. The electrochemical sensor has been successfully calibrated in the range 10-640 µg/L, obtaining a root mean square error in cross validation (RMSECV) of only 1.6 µg/L. Fresh and defrosted samples of cod, sea bream and blue whiting fish have been correctly discriminated. This proof-of-concept work has demonstrated the feasibility of the proposed application which must be replicated in a real scenario.


Assuntos
Iodetos , Iodo , Animais , Criança , Humanos , Iodatos , Alimentos Marinhos/análise , Água do Mar
4.
Molecules ; 26(12)2021 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-34205746

RESUMO

This work addressed the preservative behaviour of different icing media containing extracts from the alga Bifurcaria bifurcata. A comparative study of the antimicrobial and antioxidant effects of aqueous and ethanolic extracts of this macroalga was carried out. Whole hake (Merluccius merluccius) pieces were stored in ice containing either kind of extract and analysed for quality changes throughout a 13-day storage period. A progressive loss of microbial and biochemical quality was detected in all batches as chilling time increased. A significant inhibitory effect (p < 0.05) on microbial activity could be observed as a result of including the aqueous (lowering of psychrotrophic and lipolytic counts and pH value) and ethanolic (lowering of psychrotrophic and lipolytic counts) extracts. Additionally, both kinds of extract led to a substantial inhibition (p < 0.05) in the lipid hydrolysis rate (formation of free fatty acids), greater in the case of the batch containing ethanolic extract. Concerning lipid oxidation, a similar inhibitory effect (p < 0.05) on the formation of secondary compounds (thiobarbituric acid substances) was noticed in fish specimens corresponding to both alga extracts; however, more (p < 0.05) peroxide formation was detected in fish corresponding to the ethanolic extract batch. A preservative effect can be concluded for both kinds of extract; this effect agrees with previous studies reporting the presence of hydrophilic and lipophilic bioactive compounds in B. bifurcata.


Assuntos
Produtos Biológicos/farmacologia , Conservantes de Alimentos/farmacologia , Feófitas/efeitos dos fármacos , Alga Marinha/química , Animais , Antioxidantes/farmacologia , Produtos Biológicos/química , Etanol/química , Peixes , Conservação de Alimentos/métodos , Gadiformes , Oxirredução/efeitos dos fármacos , Alimentos Marinhos , Água/química
5.
Nutrients ; 13(5)2021 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-34065043

RESUMO

Irregular dietary intakes impairs estimations from food records. Biomarkers and method combinations can be used to improve estimates. Our aim was to examine reproducibility from two assessment methods, compare them, and validate intakes against objective biomarkers. We used the Malmö Offspring Study (55% women, 18-71 y) with data from a 4-day food record (4DFR) and a short food frequency questionnaire (SFFQ) to compare (1) repeated intakes (n = 180), (2) intakes from 4DFR and SFFQ (n = 1601), and (3) intakes of fatty fish, fruits and vegetables, and citrus with plasma biomarkers (n = 1433) (3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid [CMPF], ß-carotene and proline betaine). We also combined 4DFR and SFFQ estimates using principal component analysis (PCA). Moderate correlations were seen between repeated intakes (4DFR median ρ = 0.41, SFFQ median ρ = 0.59) although lower for specific 4DFR-items, especially fatty/lean fish (ρ ≤ 0.08). Between-method correlations (median ρ = 0.33) were higher for intakes of overall food groups compared to specific foods. PCA scores for citrus (proline betaine ρ = 0.53) and fruits and vegetables (ß-carotene: ρ = 0.39) showed the highest biomarker correlations, whereas fatty fish intake from the SFFQ per se showed the highest correlation with CMPF (ρ = 0.46). To conclude, the reproducibility of SFFQ data was superior to 4DFR data regarding irregularly consumed foods. Method combination could slightly improve fruit and vegetable estimates, whereas SFFQ data gave most valid fatty fish intake.


Assuntos
Registros de Dieta , Inquéritos sobre Dietas/estatística & dados numéricos , Dieta/estatística & dados numéricos , Adolescente , Adulto , Idoso , Biomarcadores/sangue , Estudos de Coortes , Inquéritos sobre Dietas/normas , Ingestão de Alimentos , Feminino , Frutas , Furanos/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Análise de Componente Principal , Prolina/análogos & derivados , Prolina/sangue , Propionatos/sangue , Reprodutibilidade dos Testes , Alimentos Marinhos , Verduras , Adulto Jovem , beta Caroteno/sangue
6.
N Z Med J ; 134(1536): 100-104, 2021 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-34140716

RESUMO

Ciguatera poisoning has caused illnesses in New Zealand through the consumption of contaminated reef fish imported from Pacific Islands. In May 2020 five people became ill and one was hospitalised following the consumption of Fiji Kawakawa (camouflage grouper; Epinephelus polyphekadion). The fish was purchased in New Zealand but imported from Fiji. The meal remnants were analysed for ciguatoxins, the causative compounds of ciguatera poisoning, and showed the presence of the three main toxic fish metabolites. Other fish tested from the same shipment did not contain detectable levels of ciguatoxins, indicating they were likely not toxic.


Assuntos
Bass , Intoxicação por Ciguatera , Ciguatoxinas/análise , Alimentos Marinhos , Adulto , Animais , Intoxicação por Ciguatera/diagnóstico , Intoxicação por Ciguatera/terapia , Fiji , Humanos , Masculino , Pessoa de Meia-Idade , Nova Zelândia , Alimentos Marinhos/efeitos adversos , Alimentos Marinhos/análise , Adulto Jovem
7.
Parasitol Res ; 120(7): 2493-2503, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34115215

RESUMO

Recent anecdotal reports from seafood processors in eastern Australia have described an increased occurrence of post-mortem myoliquefaction ('jellymeat') in broadbill swordfish Xiphias gladius, and macroscopic cysts throughout the musculature of yellowfin tuna Thunnus albacares. A genus of parasitic cnidarians, Kudoa (Myxosporea, Multivalvulida), species of which are known to occur in economically important wild-caught fish species globally, can cause similar quality-deterioration issues. However, Kudoa sp. epizootiology within commercially harvested, high-value fish caught within Australia is poorly understood, despite the parasite's economic importance. To determine the causative agent responsible for the observed quality deterioration in swordfish and yellowfin tuna, muscle-tissue samples from seafood processors in Mooloolaba, Australia, collected from October 2019-February 2020, were examined for parasitic infection. Kudoid myxospores were identified from both hosts and were subquadrate in shape, with four equal-sized polar capsules. The SSU rDNA sequences from both fish shared > 99% identity to Kudoa species. Kudoa musculoliquefaciens was isolated from 87.1% of swordfish sampled, suggesting that it is a widespread parasite in swordfish from the southwest Pacific Ocean. This study provides the first molecular and morphological characterisation of Kudoa thunni in yellowfin tuna and K. musculoliquefaciens in swordfish harvested from the waters of eastern Australia, expanding the geographical distribution of K. thunni and K. musculoliquefaciens to include the Coral and Tasman Seas. We demonstrate that not all infected swordfish progress to jellymeat, show the usefulness of molecular tools for reliably identifying infection by Kudoa spp., and add to the overall knowledge of kudoid epizootiology in wild-caught fish.


Assuntos
Peixes/parasitologia , Myxozoa/classificação , Atum/parasitologia , Animais , Austrália , DNA Ribossômico/genética , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Músculos/parasitologia , Myxozoa/anatomia & histologia , Myxozoa/genética , Oceano Pacífico , Doenças Parasitárias em Animais/epidemiologia , Doenças Parasitárias em Animais/parasitologia , Filogenia , RNA Ribossômico 18S/genética , RNA Ribossômico 28S/genética , Alimentos Marinhos/parasitologia , Especificidade da Espécie
8.
Environ Sci Pollut Res Int ; 28(26): 35305-35315, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34128164

RESUMO

This study assesses the PAH and heavy metal levels in muscle of warty crabs (Eriphia verrucosa), from the northern coast of the Campania region improving the data on toxic contaminants in this crustacean. The results showed a minimal PAH contamination; the mean concentrations were as follows: 0.2, 1.6 and 1.7 µg kg-1 wet weight (ww) for BaP, PAH4 and PAH6, respectively. Regarding the levels of the two PAHs not included in the European regulations, the BkF mean concentration was 0.1 µg kg-1 ww, while DahA was detected only in 10.7% of samples. Pb and Cr were also detected at low levels with mean values of 0.068 and 0.468 mg kg-1 ww, respectively; instead, high As levels, with a mean value of 5.021 mg kg-1 ww, were found. Considering the EWIs and the ILCRs calculated in this study, the PAH, Pb and Cr contamination levels found in the edible part of the crabs resulted safe for human consumption. Contrariwise, the ILCR calculated for the As exceeded the acceptable level of cancer risk, although the calculation did not refer to the inorganic form which is the only one recognized as carcinogenic. Hence, this study shows that warty crabs can accumulate environmental contaminants in their muscle tissue representing an important route of exposure to these toxics for the local population that regularly consumes them. This finding highlights the importance of monitoring the presence of these pollutants in crabs and in general in all fish and seafood in order to ensure food safety for consumers.


Assuntos
Arsênio , Braquiúros , Hidrocarbonetos Policíclicos Aromáticos , Animais , Cromo , Monitoramento Ambiental , Contaminação de Alimentos/análise , Humanos , Chumbo , Hidrocarbonetos Policíclicos Aromáticos/análise , Medição de Risco , Alimentos Marinhos/análise
9.
Mar Environ Res ; 169: 105381, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34139650

RESUMO

We investigated the main drivers of eggs and larvae distributions of European sardine and anchovy from the NW Mediterranean Sea. We used Generalized Additive Models and satellite environmental data. Mainly sea surface temperature, but also currents, surface height, and primary production were significantly correlated with both species' early stages distributions. Anchovy optimal temperature upper limit was not detected, but sardine eggs and larvae presented a small-ranged bell-shape curve relationship to SST with an upper SST threshold around 13 °C. Sardine spawning during winter appeared to be dependant not only on in-situ environmental conditions but also on summer conditions prior to the spawning event. Model predictions of the larval and spawning habitat distribution showed clear differences between developmental stages and between species, confirming a worsening of the sardine habitat with time. Considering the further increase of surface temperature predicted in the years to come, the survival of the sardine in the region could be compromised.


Assuntos
Ecossistema , Peixes , Animais , Larva , Mar Mediterrâneo , Alimentos Marinhos
10.
Mar Environ Res ; 169: 105399, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34175523

RESUMO

Overfishing is a key stressor in many coral reefs, affecting their function and ecosystem services. In the Gulf of California, 50 years of artisanal and recreational fishing have removed most of the largest and most vulnerable fish species, the Espiritu Santo Archipelago (ESA) is a marine protected area (MPA) where artisanal and recreational fishing are allowed in most of the natural reefs, while the artificial reefs (wrecks) are fully protected. An important question under such circumstances is whether artificial reefs can serve as surrogate habitats for the natural reefs. To address this question, we characterized the fish community (species richness, abundance, differences in taxonomic structure) and analyzed the obtained data by multivariate ordination and similarity analysis. The highest species richness was found in the artificial reefs, with total of 83 species, of which 21 species were exclusive. Contrary to the species richness, the total fish abundance was 20% higher at the natural reefs. The fish assemblages of the artificial reefs differ significantly from those of the natural reefs. The natural reefs of the ESA are dominated by few fish species from families of lower trophic levels. In contrast, the studied shipwrecks provide refuge to commercially important fish species (such as Snappers, Triggerfish, Jacks, and Groupers) including threatened species. The different fish compositions on the natural and artificial reefs is likely to be the outcome of differential fishing pressure. The non-fished areas (wrecks) of the ESA may represent a good strategy for reef restoration, enhanced fishing grounds (via spillover) and fishing management. However, the fish community structure of the artificial reefs differs from natural rocky reefs of the ESA, and therefore, should not be considered as surrogate habitats for natural reefs. The results point out the need to reassess the management effectiveness of the natural reefs of the ESA.


Assuntos
Conservação dos Recursos Naturais , Ecossistema , Animais , Recifes de Corais , Pesqueiros , Peixes , Alimentos Marinhos
11.
Molecules ; 26(11)2021 May 22.
Artigo em Inglês | MEDLINE | ID: mdl-34067234

RESUMO

There is consistent evidence that long-chain polyunsaturated fatty acids (LCPUFA) belonging to the n-3 series, i.e., eicosapentaenoic (20:5n-3, EPA) and docosahexaenoic (22:6n-3, DHA) acids, decrease the risk of heart, circulatory and inflammatory diseases. Furthermore, the bioavailability of such fatty acids has been shown to depend on their location in triacylglycerol (TG) molecules at the sn-2 position. Consequently, great attention has been accorded to the synthesis of structured acylglycerols (sAG), which include EPA or DHA at the sn-2 position. The aim of this work was to synthesize sAG starting from deodorized refined commercial salmon oil. For this, immobilized lipase B from Candida antarctica (nonspecific) was used as a catalyst for the intra-interesterification process under CO2 supercritical conditions (CO2SC). According to the CO2SC reaction time, three different fractions including sAG compounds were obtained. The location of EPA and DHA at the sn-2 position in the resulting glycerol backbone was identified by mass spectrometry (MALDI-TOF) analysis. In all fractions obtained, a marked decrease in the starting TG content was observed, while an increase in the DHA content at the sn-2 position was detected. The fraction obtained after the longest reaction time period (2 h) led to the highest yield of sn-2 position DHA in the resulting sAG molecule.


Assuntos
Ácidos Docosa-Hexaenoicos/química , Ácido Eicosapentaenoico/química , Óleos de Peixe/química , Glicerídeos/síntese química , Triglicerídeos/química , Animais , Basidiomycota , Catálise , Técnicas de Química Analítica , Chile , Cromatografia em Camada Delgada , Esterificação , Ésteres/química , Ácidos Graxos/química , Humanos , Hidrólise , Lipase/química , Lipídeos/química , Espectrometria de Massas , Probabilidade , Reprodutibilidade dos Testes , Alimentos Marinhos/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
12.
Ecotoxicol Environ Saf ; 221: 112446, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34175823

RESUMO

Lipophilic shellfish toxins (LSTs) can cause human illness and therefore represent a serious threat to public health. Shellfish are the main dietary source of LSTs, but very few studies have appraised dietary exposure to LSTs through shellfish consumption in China. We measured levels of multiple LSTs in shellfish samples sold in the principal wholesale seafood market in the southern coastal city of Shenzhen, and we estimated the potential for acute and chronic LST exposure of the Shenzhen population via ingestion of shellfish. LST contamination data were obtained from a total of 14 species of 188 commercial samples. Eleven individual LSTs, namely okadaic acid (OA), dinophysis toxin-1 and -2 (DTX1 and DTX2), pectenotoxin-2 (PTX2), yessotoxin and homo yessotoxin (YTX and hYTX), azaspiracid-1, -2 and -3 (AZA1, AZA2, AZA3), spirolides (SPXs), and gymnodimine (GYM), were determined using liquid chromatography electrospray-ionization tandem mass spectrometry (LC-ESI-MS/MS). More than two thirds of samples showed undetectable LSTs, while the detection rates (the proportion of samples with detectable LSTs) of individual LSTs ranged from 0% to 45.7%. Most shellfish samples had lower levels of LST contamination than the corresponding limits of detection (LODs), while some samples had levels of hYTX and GYM that exceeded the limits of quantification (LOQs). Overall, levels of LSTs in the 188 samples were below the regulatory limits set by most countries. Acute and chronic exposures of LST were estimated by a point-estimate modeling method that combined sample contamination data with consumption data from dietary survey of Shenzhen residents and consumption figures proposed by EFSA, the European Food Safety Authority. Seasonal variations in LST concentrations were noted in some instances. Overall, the estimated acute exposure to LSTs based on consumption of large-size shellfish portions and the maximum LSTs contamination level were below the provisional acute reference doses (ARfDs) proposed by the EFSA. Chronic exposure estimates based on mean and 99th percentile consumption of shellfish by Shenzhen residents and mean LSTs contamination levels in the collected samples were from 2452 to 74 times lower than those associated with estimated acute exposure levels.


Assuntos
Exposição Dietética/análise , Exposição Dietética/estatística & dados numéricos , Contaminação de Alimentos/análise , Contaminação de Alimentos/estatística & dados numéricos , Frutos do Mar/análise , Animais , Cromatografia Líquida , Dinoflagelados/química , Humanos , Toxinas Marinhas/análise , Alimentos Marinhos/análise , Espectrometria de Massas em Tandem
13.
J Chromatogr A ; 1651: 462328, 2021 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-34153733

RESUMO

Paralytic shellfish toxins and tetrodotoxin (puffer-fish toxin), the latter of which was recently found in bivalves from Europe, Japan, and New Zealand, are potent neurotoxins. A simple and effective clean-up procedure was developed for the simultaneous determination of ten paralytic shellfish toxins (gonyautoxins 1-6, decarbamoylgonyautoxins 2 and 3, and N-sulfocarbamoylgonyautoxins 2 and 3) and tetrodotoxin in the scallop, Mizuhopecten (Patinopecten) yessoensis, and the short-necked clam, Ruditapes philippinarum. To reduce matrix effects, 1% aqueous acetic acid extracts of the bivalves were cleaned up by ion-pair solid-phase extraction using a graphite carbon cartridge with tridecafluoroheptanoic acid as the volatile ion-pair reagent, followed by fourfold dilution. The ten paralytic shellfish toxins and tetrodotoxin were then separated on a hydrophilic interaction chromatography column and quantified by tandem mass spectrometry. The limits of detection and the limits of quantification for the ten PSTs ranged from 0.09 to 13.0 µg saxitoxin equivalents/kg and from 0.26 to 39.4 µg saxitoxin equivalents/kg, respectively. The limit of detection and the limit of quantification for tetrodotoxin ranged from 27.4 to 27.9 µg/kg and from 83.1 to 84.4 µg/kg, respectively. The proposed method yielded minimal matrix effects for the 11 analytes, thus allowing their quantification by simple external calibration. The proposed method also gave good mean recoveries of the 11 analytes ranging from 75.7 to 96.2% with relative standard deviations less than 16% at three fortification levels for the ten paralytic shellfish toxins (total concentrations of 277, 554, and 1107 µg saxitoxin equivalents/kg) and tetrodotoxin (100, 200, and 400 µg/kg) in the two bivalve samples. Finally, the proposed method was applied for the determination of the ten paralytic shellfish toxins and tetrodotoxin in scallop and short-necked clam samples.


Assuntos
Bivalves/química , Pectinidae/química , Saxitoxina/análogos & derivados , Extração em Fase Sólida , Espectrometria de Massas em Tandem , Tetrodotoxina/análise , Animais , Europa (Continente) , Grafite/química , Interações Hidrofóbicas e Hidrofílicas , Saxitoxina/análise , Alimentos Marinhos/análise , Tetrodotoxina/isolamento & purificação
14.
Food Chem ; 362: 130214, 2021 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-34082293

RESUMO

The ionic covalent organic framework (TPB-BFBIm-iCOF) was facilely synthetized by the size-controllable confinement method and chosen as the online solid phase extraction (SPE) adsorbent. This adsorbent showed fast adsorption equilibrium (5 min) and high adsorption capacity (87.7-140.8 mg g-1) for the per- and polyfluorinated alkyl substances (PFASs). The TPB-BFBIm-iCOF microsphere revealed the satisfactory enrichment performance for PFASs by means of the electrostatic interaction, hydrophobic effect and ordered channel structure. After extraction, the loaded TPB-BFBIm-iCOF-online SPE column was eluted and applied to the ultrahigh performance liquid chromatography tandem mass spectrometry analysis. Under the optimum conditions, the method displayed satisfactory linearity (R2 ≥ 0.9910) and low limits of detection (≤0.0017 ng g-1) for five seafoods. The relative recoveries of PFASs were 85.3%-109.4% with the relative standard deviation ≤ 9.9%. The method exhibited potential value in monitoring the toxicokinetics and environmental behaviors of PFASs.


Assuntos
Contaminação de Alimentos/análise , Hidrocarbonetos Fluorados/análise , Alimentos Marinhos/análise , Extração em Fase Sólida/métodos , Adsorção , Cromatografia Líquida de Alta Pressão/métodos , Teoria da Densidade Funcional , Análise de Alimentos/métodos , Hidrocarbonetos Fluorados/química , Limite de Detecção , Espectrometria de Massas em Tandem/métodos
15.
Talanta ; 232: 122504, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-34074453

RESUMO

A method based on asymmetric flow field-flow fractionation (AF4) coupled to ultraviolet-visible (UV-vis) spectroscopy and inductively coupled plasma mass spectrometry (ICP-MS) has been developed for silver nanoparticles (Ag NPs) detection and quantification in bivalve molluscs. Samples were pre-treated using a conventional enzymatic (pancreatin and lipase) hydrolysis procedure (37 °C, 12 h). AF4 was performed using a regenerated cellulose (RC) membrane (10 kDa, 350 µm spacer) and aqueous 5 mM Tris-HCl pH = 7.4 as carrier. AF4 separation was achieved with a program that included a focusing step with tip and focus flows of 0.20 and 3.0 mL min-1, respectively, and an injection time of 4.0 min. Elution of different size fractions was performed using a cross flow of 3.0 mL min-1 for 15 min, followed by linear cross flow decrease for 7.5 min, and a washing step for 9.4 min with no cross flow. Several bivalve molluscs (clams, oysters and variegated scallops) were analysed for total Ag content (ICP-MS after microwave assisted acid digestion), and for Ag NPs by the method presented here. Results show that Ag NPs are detected at the same elution time than proteins (UV monitoring at 280 and 405 nm), which suggests a certain interaction occurred between Ag NPs with proteins in the enzymatic extracts. AF4-UV-ICP-MS fractograms also suggest different Ag NPs size distributions for selected samples. Membrane recoveries, determined by peak area comparison of fractograms with and without application of cross flow, were within the 49-121% range. Confirmation of the presence Ag NPs in the investigated enzymatic extracts was demonstrated by SEM after an oxidative pre-treatment based on hydrogen peroxide and microwave irradiation.


Assuntos
Fracionamento por Campo e Fluxo , Nanopartículas Metálicas , Hidrólise , Espectrometria de Massas , Nanopartículas Metálicas/análise , Tamanho da Partícula , Alimentos Marinhos , Prata , Análise Espectral
16.
An Acad Bras Cienc ; 93(2): e20190715, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34076036

RESUMO

Spiny lobsters (Family Palinuridae) are a popular seafood in Brazil. We characterize the fishing activity and analyze the population of lobster species captured in the coast of the state of Rio Grande do Norte, Northeast Brazil. We monitored the landings of lobster fishermen at the start of the fishing season, from May 24 to November 21, in 2010, at Pirangi beach, the main landing point in the metropolitan area of Natal. The lobster fisherman in the region use small vessels and a compressor to dive in artificial reefs known as marambaias. The artisanal fishermen are engaged in several illegal practices and revealed a greater capture of lobsters categorized as small-sized for the market (11-13.95cm tail length). The capture area led to different fishing pressures for each species. For P. laevicauda, fishing pressure occurred for all size classes, while for P. meripurpuratus, pressure occurred partially, only for a few population cohorts. On regional scale, fishing involved a demand-supply trade balance centered on the size of capture vs. abundance, regardless of the natural interespecific differences on body size and the preferential distribution area. Our results provide key information for future decision making involving the fishing of spiny lobster.


Assuntos
Decápodes , Palinuridae , Animais , Brasil , Pesqueiros , Alimentos Marinhos
17.
J Agric Food Chem ; 69(24): 6879-6887, 2021 Jun 23.
Artigo em Inglês | MEDLINE | ID: mdl-34105975

RESUMO

DNA-based detection methods are highly promising for risk assessment in the food sector, such as tracing the existence of food allergens. However, due to the complexity of food matrices, cumbersome protocols are often needed to isolate the DNA components, which hinder the achievement of rapid and on-site detection. Herein, an instant and multiple DNA extraction method was developed based on the poly(vinyl alcohol) microneedle (MN) patch. With simple press and peel-off operations within 1 min, samples suitable for DNA-based analysis such as polymerase chain reaction (PCR) could be collected. By further combining with the recombinase polymerase amplification assay, rapid screening of the allergenic risks in complex samples such as shrimp ball and cheesecake could be achieved within 30 min. The MN-based DNA extraction method not only was a potential alternative to the traditional DNA extraction method but provided a transformative approach in realizing rapid, on-site detection of foodborne hazards in collaborating with fast DNA-based assays.


Assuntos
DNA , Alimentos Marinhos , Alérgenos , DNA/genética , Reação em Cadeia da Polimerase , Alimentos Marinhos/análise
18.
Mar Environ Res ; 169: 105362, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-34052594

RESUMO

Understanding how marine fish early-life history is affected in the long-term by environmental and oceanographic factors is fundamental given its importance to population dynamics and connectivity. This work aimed at determining the influence of these processes on the interannual variability in hatch day and early-life growth patterns of European seabass, over a seven-year period (2011-2017) in the Atlantic Iberian coast. To accomplish this, otolith microstructure analysis was used to determine seabass hatch day and to develop early-growth correlations. In most years, hatching occurred from February to April, with two exceptions: in 2012, hatching started in early-January, and in 2016 an exceptionally long hatching period was registered. Using generalized additive models (GAM), we observed that sea surface temperature (SST), the North Atlantic Oscillation index (NAOi) and Chlorophyll-a (Chla) were the main drivers behind the inter-annual variability in seabass hatch day. Analysis of correlations between growth increments allowed assessing important periods of seabass growth and how future growth is affected. Since seawater temperature is among the main drivers for seabass recruitment and growth, its life cycle may be hampered due to ocean warming and an increasingly unstable climate, with consequences for the natural marine stocks and their harvest.


Assuntos
Bass , Animais , Clima , Oceanos e Mares , Alimentos Marinhos/análise , Água do Mar , Temperatura
19.
Wei Sheng Yan Jiu ; 50(2): 296-300, 2021 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-33985640

RESUMO

OBJECTIVE: Ultra-fast liquid chromatography-tandem mass spectrometry method was developed for the analysis of contamination degree of biotoxins in seafood in Ningbo City from 2017 to 2019 and the assessment of dietary exposure was conducted. METHODS: Samples were extracted and purified with optimized pretreatment process and then injected for analysis. According to the result of the measurements, an international point estimate model was used to evaluate the dietary exposure of the population. RESULTS: For tetrodotoxin and 16 shellfish toxins monitored routinely, gonyautoxin5(GTX5), tetrodotoxin and homo-yessotoxin(hYTX) had higher detection rate, other toxins including okadaic acid(OA), dinophysistoxin1(DTX1), decarbamoyl gonyautoxin2(dcGTX2) and decarbamoyl gonyautoxin3(dcGTX3) were detected sporadically. The detection rates of TTX、GTX and hYTX were 27%, 52% and 12%, respectively. The concentration ranges of TTX, GTX and hYTX in polluted samples were 0. 003-0. 535, 0. 008-0. 189 and 0. 032-0. 110 mg/kg. The exposure risk indices(ERI) of TTX, GTX5, hYTX, dcGTX2 and dcGTX3 were 2. 5, 0. 026, 0. 0080, 0. 79 and 0. 32, respectively. CONCLUSION: Marine biotoxins have a lower dietary health risk to the population. It is must be given great attention that in the season of toxic red tide, the detection rates of higher toxic toxins, dcGTX2 and dcGTX3 increased significantly with high risks to human. Moreover, the dietary health risk of tetrodotoxin in routine surveillance in 2019 was higher.


Assuntos
Exposição Dietética , Toxinas Marinhas , Cromatografia Líquida , Humanos , Toxinas Marinhas/análise , Toxinas Marinhas/toxicidade , Ácido Okadáico/análise , Alimentos Marinhos/análise
20.
Spectrochim Acta A Mol Biomol Spectrosc ; 260: 119985, 2021 Nov 05.
Artigo em Inglês | MEDLINE | ID: mdl-34058667

RESUMO

The objective of this study was to assess the ability of utilizing attenuated total reflection mid-infrared (ATR-MIR) spectroscopy in combination with machine learning techniques to classify the presence of different types of microplastics in artificially adulterated fish and seafood samples. Different polymers namely poly-vinyl chloride (PVC), polycarbonate (PC), polystyrene (PS), polypropylene (PP) and low (LDPE) and high-density polyethylene (HDPE) were mixed with homogenized fish and seafood samples. Homogenized samples were analyzed using MIR spectroscopy and classification models developed using machine learning algorithms such as partial least squares discriminant analysis (PLS-DA). The results of this study revealed that it was possible to identify between adulterated and non-adulterated samples as well as the different microplastic types added to the homogenized samples using ATR-MIR spectroscopy. This study confirmed the ability of combining machine learning methods with ATR-MIR spectroscopy to directly analyze microplastic adulteration in fleshy foods such as fish and seafood. This proof-of-concept study can be utilized and extended to monitor the presence of plastics either in a wide range of fleshy foods or along the entire food value chain.


Assuntos
Microplásticos , Plásticos , Animais , Análise dos Mínimos Quadrados , Aprendizado de Máquina , Alimentos Marinhos
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