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1.
Shokuhin Eiseigaku Zasshi ; 58(1): 32-35, 2017.
Artigo em Japonês | MEDLINE | ID: mdl-28260730

RESUMO

Kuwazuimo (Alocasia odora) and shimakuwazuimo (Alocasia cucullata) are evergreen perennial plants that originated in East Asia. Although inedible, they are occasionally eaten by mistake because they resemble satoimo (Colocasia esculenta), and this has caused food poisoning in Japan. It is not easy to determine the cause of a food poisoning outbreak from the shape or chemical composition when the available sample is small. Therefore, we developed a new primer pair for PCR to identify kuwazuimo and shimakuwazuimo in small samples, based on the internal transcribed spacer (ITS) region of ribosomal DNA. Using PCR with the developed primer pair, we detected all samples of kuwazuimo obtained from the market, while excluding 17 other kinds of crops. The samples were identified as shimakuwazuimo by DNA sequencing of the PCR products. The present PCR method showed high specificity and was confirmed to be applicable to the identification of kuwazuimo and shimakuwazuimo from various crops.


Assuntos
Alocasia/química , Alocasia/genética , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Reação em Cadeia da Polimerase/métodos , Alocasia/envenenamento , DNA Ribossômico , Doenças Transmitidas por Alimentos/etiologia , Doenças Transmitidas por Alimentos/prevenção & controle , Sensibilidade e Especificidade , Análise de Sequência de DNA
2.
Mitochondrial DNA A DNA Mapp Seq Anal ; 27(5): 3464-5, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-26258514

RESUMO

The complete chloroplast sequence of Alocasia macrorrhizos is 154 995 bp in length, containing a pair of inverted repeats of 25 944 bp separated by a large single-copy (LSC) region and a small single-copy (SSC) region of 87 366 bp and 15 741 bp, respectively. The chloroplast genome encodes 132 predicted functional genes, including 87 protein-coding genes, four ribosomal RNA genes, and 37 transfer RNA genes, 18 of which are duplicated in the inverted repeat regions. In these genes, 16 genes contained single intron and two genes comprising double introns. A maximum-likelihood phylogenetic analysis using complete chloroplast genome revealed that A. macrorrhizos does not belong to Araceae family, which infers that the A. macrorrhizos is distant from the species in Araceae family.


Assuntos
Alocasia/genética , Cloroplastos/genética , Genoma de Cloroplastos , Análise de Sequência de DNA/métodos , Composição de Bases , DNA Ribossômico/genética , Ordem dos Genes , Tamanho do Genoma , Filogenia , RNA de Transferência/genética
3.
Mol Phylogenet Evol ; 63(1): 43-51, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22209857

RESUMO

Alocasia comprises over 113 species of rainforest understorey plants in Southeast Asia, the Malesian region, and Australia. Several species, including giant taro, Alocasia macrorrhizos, and Chinese taro, Alocasia cucullata, are important food plants or ornamentals. We investigated the biogeography of this genus using plastid and nuclear DNA sequences (5200 nucleotides) from 78 accessions representing 71 species, plus 25 species representing 16 genera of the Pistia clade to which Alocasia belongs. Divergence times were inferred under strict and relaxed clock models, and ancestral areas with Bayesian and maximum likelihood approaches. Alocasia is monophyletic and sister to Colocasiagigantea from the SE Asian mainland, whereas the type species of Colocasia groups with Steudnera and Remusatia, requiring taxonomic realignments. Nuclear and plastid trees show topological conflict, with the nuclear tree reflecting morphological similarities, the plastid tree species' geographic proximity, suggesting chloroplast capture. The ancestor of Alocasia diverged from its mainland sister group c. 24 million years ago, and Borneo then played a central role in the expansion of Alocasia: 11-13 of 18-19 inferred dispersal events originated on Borneo. The Philippines were reached from Borneo 4-5 times in the Late Miocene and Early Pliocene, and the Asian mainland 6-7 times in the Pliocene. Domesticated giant taro originated on the Philippines, Chinese taro on the Asian mainland.


Assuntos
Alocasia/genética , Evolução Biológica , Filogenia , Alocasia/classificação , Ásia Sudeste , Austrália , Teorema de Bayes , Bornéu , Núcleo Celular/genética , DNA de Cloroplastos/genética , DNA de Plantas/genética , Funções Verossimilhança , Filipinas , Análise de Sequência de DNA
4.
Artigo em Inglês | MEDLINE | ID: mdl-17167199

RESUMO

The cDNA of Alocasia macrorrhiza lectin (aml, GenBank accession number: DQ340864) was cloned by RACE-PCR and its characteristics were predicted by various bioinformatics tools. GSPs (Gene Specific Primers) were designed according to the conserved regions of the genes encoded for lectins and similar proteins from the same family Araceae. Total RNAs were extracted from the tubers of A macrorrhiza by Qiagen RNeasy mini kit. The 3'- and 5'-RACE-PCRs were performed with the isolated total RNAs by SMART(TM)RACE cDNA amplification kit from BD Biosciences Clontech Company, respectively. The purified PCR products were ligated with pMD 18-T vector, and the confirmed clones were sequenced. The full-length cDNA of aml was obtained by combination of 3'- and 5'-end sequences, and was then confirmed by full-length 3'-RACE-PCR. The aml cDNA is 1 124 bp long. The deduced amino acid length of AML lectin is 270 aa. Its relative molecular weight is 29.7 kD. The results of homologous analysis showed a high similarity between AML and other mannose-binding lectins and similar proteins from Araceae family. Two typical B-lectin domains and three mannose- binding motifs were found in the sequence of AML. With all these taken together, it can be concluded that this newly cloned aml cDNA encodes for a mannose-binding lectin.


Assuntos
Alocasia/genética , DNA Complementar/genética , Lectinas de Plantas/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , Biologia Computacional , DNA Complementar/química , Modelos Moleculares , Dados de Sequência Molecular , Filogenia , Lectinas de Plantas/química , Lectinas de Plantas/classificação , Estrutura Secundária de Proteína , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos
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