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1.
Int J Radiat Biol ; 95(11): 1529-1542, 2019 11.
Artigo em Inglês | MEDLINE | ID: mdl-31314632

RESUMO

Evaluation of the modulatory effect of ethanolic extract of Alocasia indica tuber (EEAIT) against γ-irradiation induced ovarian and uterine toxicity. Extract preparation was done by 80% hydro-ethanol using Soxhlet apparatus. EEAIT was administered to female Swiss albino mice (n = 5) daily (200 and 400 mg/kg body weight/d) for 7 days before γ-irradiation exposure (2.9 Gy). FSH, LH, estrogen, progesterone, cytokine levels, and oxidative stress parameters were measured after 24 hours of γ-irradiation. Histology, folliculogenesis, viability of granulosa cells, ROS measurement by flow cytometry, western blot of P450scc, P45017A1, 3ß HSD and SF 1 were also performed. In addition, fertility status was assessed by fecundability and fecundity. The results showed that EEAIT exhibit a strong radioprotective activity by reducing the oxidative stress and thereby restored the ovarian and uterine alterations. EEAIT also improved the abnormality in follicle development, restored altered gonadal hormones and cytokines levels, increase the fertility status, reducing ROS level of granulosa cells with increasing granulosa cells viability and steroidogenic enzyme activity as compared to control. So EEAIT showed a radioprotective effect on γ-irradiation induced ovarian and uterine damage. Our results suggested that Alocasia indica tuber can be a potential radioprotector to prevent female infertility.


Assuntos
Alocasia/química , Ovário/efeitos dos fármacos , Extratos Vegetais/farmacologia , Lesões por Radiação/prevenção & controle , Protetores contra Radiação/farmacologia , Útero/efeitos dos fármacos , Animais , Antioxidantes/metabolismo , Catalase/metabolismo , Sobrevivência Celular/efeitos da radiação , Citocinas/metabolismo , Etanol/química , Feminino , Fertilidade/efeitos da radiação , Raios gama , Células da Granulosa/efeitos da radiação , Malondialdeído/metabolismo , Camundongos , Óxido Nítrico/metabolismo , Ovário/efeitos da radiação , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/metabolismo , Útero/efeitos da radiação
2.
Int J Biol Macromol ; 113: 681-691, 2018 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29505868

RESUMO

Protease inhibitors from plants play major role in defensive mechanism against various pathogenic organisms. AMTIN from the tubers of Alocasia macrorrhiza has been purified and characterized as multi-functional Kunitz type protease inhibitor. AMTIN is varied from other KTIs by having three different loops specific for binding to trypsin/amylase and subtilisin that are located approximately 30Ǻ away from one another as evidenced from crystallographic efforts. Biochemical studies on AMTIN reveal simultaneous binding of protease/amylase and have been cross validated using in-silico tools to model Amylase - AMTIN - Trypsin complex without any steric clashes. Apart from multi functionality, the remarkable structural and functional stability of AMTIN at high temperature, presence of many phosphorylation, myristoylation and glycosylation sites and molecular docking studies with dengue viral protease (NS2B-NS3) makes this protein interesting. Hence AMTIN can be considered as a template to design effective antivirals against dengue virus.


Assuntos
Alocasia/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Inibidores de Proteases/química , Inibidores de Proteases/farmacologia , Serina Endopeptidases/metabolismo , Proteínas não Estruturais Virais/antagonistas & inibidores , Sequência de Aminoácidos , Simulação de Acoplamento Molecular , Extratos Vegetais/metabolismo , Inibidores de Proteases/metabolismo , Conformação Proteica , Serina Endopeptidases/química , Proteínas não Estruturais Virais/química , Proteínas não Estruturais Virais/metabolismo
3.
J Ethnopharmacol ; 213: 359-365, 2018 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-29180042

RESUMO

Alocasia cucullata, a Chinese herb, has been used as an anticancer treatment in southern China. Phosphatase and tensin (PTEN), is a tumor suppressor gene and the loss of PTEN expression may activate the phosphoinositide-3-kinase (PI3K)/AKT signaling pathway which play a key role in tumors formation and progression. In this study, we evaluated the anti-melanoma effect and the underlying mechanism of 50% ethanolic extract of A. cucullata (EAC) in vitro and in vivo. Using MTT, wound healing, and transwell assays, we found that EAC suppressed the proliferation, migration, and invasion of melanoma cells (B16-F10, A375 and A2058) in a dose-dependent manner. We also found that EAC suppresses B16-F10 tumor growth in a xenografted mouse model. Western blot analysis revealed that the expression level of PTEN was up-regulated, and phosphorylation of PI3K and AKT reduced in B16-F10 cells and tumor tissues after EAC treatment. No significant differences were observed in PI3K and AKT expression. Moreover, immunohistochemistry showed that the number of PTEN-positive cells in tumor tissues increased and that of p-AKT-positive cells decreased with EAC treatment, corroborating the western blot results. Our data reveal that EAC can inhibit malignant melanoma in vitro and in vivo and suggest that its anti-tumor effect is associated with modulation of the PTEN/ PI3K/AKT signaling pathway. In summary, our findings highlight a promising herbal remedy for the treatment of malignant melanoma, which warrants further study.


Assuntos
Alocasia/química , Medicamentos de Ervas Chinesas/uso terapêutico , Melanoma/tratamento farmacológico , PTEN Fosfo-Hidrolase/biossíntese , Fosfatidilinositol 3-Quinases/metabolismo , Fitoterapia , Proteínas Proto-Oncogênicas c-akt/metabolismo , Animais , Linhagem Celular Tumoral , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Regulação para Baixo , Humanos , Camundongos , Invasividade Neoplásica , Fosforilação , Raízes de Plantas/química , Transdução de Sinais , Regulação para Cima , Ensaios Antitumorais Modelo de Xenoenxerto
4.
Phytochemistry ; 143: 81-86, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-28780427

RESUMO

Six previously undescribed piperidine alkaloids were isolated from the rhizomes of Alocasia macrorrhiza (L.) Schott. Their structures were elucidated based on 1D and 2D NMR, IR, HR-ESI-MS spectroscopic analysis and the application of a modified Mosher method. All isolated alkaloids were evaluated for cytotoxicity against five human cancer cell lines (CNE-1, Detroit 562, Fadu, MGC-803, and MCF-7) using the MTT method. Only one compound exhibited cytotoxic effects against Detroit 562, Fadu, and MCF-7 cell lines with IC50 values less than 10 µM.


Assuntos
Alcaloides/isolamento & purificação , Alocasia/química , Antineoplásicos Fitogênicos/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Piperidinas/isolamento & purificação , Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacologia , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Humanos , Células MCF-7 , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Piperidinas/química , Piperidinas/farmacologia , Rizoma/química
5.
Shokuhin Eiseigaku Zasshi ; 58(1): 32-35, 2017.
Artigo em Japonês | MEDLINE | ID: mdl-28260730

RESUMO

Kuwazuimo (Alocasia odora) and shimakuwazuimo (Alocasia cucullata) are evergreen perennial plants that originated in East Asia. Although inedible, they are occasionally eaten by mistake because they resemble satoimo (Colocasia esculenta), and this has caused food poisoning in Japan. It is not easy to determine the cause of a food poisoning outbreak from the shape or chemical composition when the available sample is small. Therefore, we developed a new primer pair for PCR to identify kuwazuimo and shimakuwazuimo in small samples, based on the internal transcribed spacer (ITS) region of ribosomal DNA. Using PCR with the developed primer pair, we detected all samples of kuwazuimo obtained from the market, while excluding 17 other kinds of crops. The samples were identified as shimakuwazuimo by DNA sequencing of the PCR products. The present PCR method showed high specificity and was confirmed to be applicable to the identification of kuwazuimo and shimakuwazuimo from various crops.


Assuntos
Alocasia/química , Alocasia/genética , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Reação em Cadeia da Polimerase/métodos , Alocasia/envenenamento , DNA Ribossômico , Doenças Transmitidas por Alimentos/etiologia , Doenças Transmitidas por Alimentos/prevenção & controle , Sensibilidade e Especificidade , Análise de Sequência de DNA
6.
Fitoterapia ; 117: 126-132, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28161134

RESUMO

Five new lignanamides (1-5), and one new monoindole alkaloid (6), along with eight known compounds (7-14) were isolated and identified from the rhizomes of Alocasia macrorrhiza (giant taro). All purified compounds were evaluated for their inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells, and the antiproliferative activities against human nasopharyngeal carcinoma epithelial (CNE-1), human gastric carcinoma (MGC-803), and human breast cancer (MCF-7) cell lines by MTT method. Compounds 2, 4, 7 and 8 exhibited significant inhibitory effects on NO production with the IC50 values of 2.35±0.38, 9.20±0.94, 3.45±0.39 and 7.96±0.56µM, respectively. The results suggested the lignanamides and monoindoles might be responsible for the anti-inflammatory activity of giant taro and might be potential anti-inflammatory candidates.


Assuntos
Alocasia/química , Anti-Inflamatórios/química , Indóis/química , Lignanas/química , Animais , Anti-Inflamatórios/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Humanos , Indóis/isolamento & purificação , Lignanas/isolamento & purificação , Lipopolissacarídeos , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Células RAW 264.7 , Rizoma/química
7.
Z Naturforsch C J Biosci ; 72(1-2): 21-25, 2017 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-27497869

RESUMO

An indole alkaloid, 2-(5-hydroxy-1H-indol-3-yl)-2-oxo-acetic acid (1) isolated for the first time from nature, in addition to the nine known compounds 5-hydroxy-1H-indole-3-carboxylic acid methyl ester (2), alocasin B (3), hyrtiosin B (4), α-monopalmitin (5), 1-O-ß-D-glucopyranosyl-(2S, 3R, 4E, 8Z)-2-[(2(R)-hydroctadecanoyl) amido]-4,8-octadecadiene-1,3-diol (6), 3-epi-betulinic acid (7), 3-epi-ursolic acid (8), ß-sitosterol (9) and ß-sitosterol 3-O-ß-D-glucoside (10) were isolated from the rhizomes of Alocasia macrorrhiza (Araceae). Their structures were elucidated by 1D and 2D NMR spectroscopic data. Of these compounds, 6 exhibited the strongest cytotoxicity against the four tested human cancer cell lines (IC50 of about 10 µM against Hep-2 larynx cancer cells).


Assuntos
Alocasia/química , Citotoxinas/análise , Alcaloides Indólicos/análise , Alocasia/toxicidade , Antineoplásicos Fitogênicos/análise , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Linhagem Celular Tumoral , Citotoxinas/isolamento & purificação , Citotoxinas/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Alcaloides Indólicos/isolamento & purificação , Alcaloides Indólicos/farmacologia , Concentração Inibidora 50 , Espectrometria de Massas , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Rizoma/química
8.
J Nat Med ; 70(3): 602-9, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27120176

RESUMO

Ten steroids and iridoids were isolated from the tubers of Alocasia cucullata (Lour.) G. Don. Among them, alocasgenin A (1) and alocasgenoside B-C (2-3) were new compounds and the aglycone of compound 1, obtained from the acid hydrolysis of 1, was named alocasgenol (1a). Also, for the first time, tenacigenin B (4), 17ß-tenacigenin-B (5), 3-O-6-deoxy-3-O-methyl-ß-D-allopyranosyl-(1→4)-ß-D-oleandropyranosyl-tenacigenin C (6), marsdenoside A-B (7-8) and tenacigenoside A-B (9-10) were isolated from the genus Alocasia. The chemical structures were elucidated by the extensive analysis of spectral data and compared with the literature. By evaluation of the cytotoxic and tyrosine kinase inhibition, compounds 1-10, 1a and compound 2 showed significant growth inhibition against two tumour cell lines, MGC-803 and HT-29, while compounds 1, 1a, 3, 6 and 8 presented moderate inhibition. Furthermore, compound 2 had the inhibitory property against the enzyme activity biochemically.


Assuntos
Alocasia/química , Iridoides/química , Proteínas Tirosina Quinases/antagonistas & inibidores , Esteroides/química , Linhagem Celular Tumoral , Humanos , Estrutura Molecular
9.
Bioresour Technol ; 217: 252-6, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26897473

RESUMO

The study was carried out to investigate the use of a high biomass plant, Pennisetum hydridum, to treat municipal sewage sludge (MSS). An experiment composed of plots with four treatments, soil, fresh sludge, soil-sludge mixture and phyto-treated sludge, was conducted. It showed that the plant could not survive directly in fresh MSS when cultivated from stem cuttings. The experiment transplanting the incubated cutting with nurse medium of P. hydridum in soil and fresh MSS, showed that the plants grew normally in fresh MSS. The pilot experiment of P. hydridum and Alocasia macrorrhiza showed that the total yield and nutrient amount of P. hydridum were 9.2 times and 3.6 times more than that of A. macrorrhiza. After plant treatment, MSS was dried, stabilized and suitable to be landfilled or incinerated, with a calorific value of about 5.6MJ/kg (compared to the initial value of 1.9MJ/kg fresh sludge).


Assuntos
Alocasia , Biodegradação Ambiental , Biomassa , Pennisetum , Esgotos/química , Alocasia/química , Alocasia/metabolismo , Pennisetum/química , Pennisetum/metabolismo
10.
J Nat Prod ; 78(12): 3080-2, 2015 Dec 24.
Artigo em Inglês | MEDLINE | ID: mdl-26625266

RESUMO

Herein is reported a synthesis of alocasin A (1), an alkaloid component of Alocasia macrorrhiza, a herbaceous plant used in folk medicine throughout southern Asia. A double Suzuki-Miyaura cross-coupling reaction between a 3-borylindole and 2,5-dibromopyrazine was used to assemble the heteroaromatic framework of the natural product. Removal of the protecting groups gave a synthetic sample of 1, the spectroscopic data of which matched those in the isolation report of this compound.


Assuntos
Alcaloides/síntese química , Alocasia/química , Produtos Biológicos/síntese química , Alcaloides/química , Produtos Biológicos/química , Medicina Tradicional , Estrutura Molecular
11.
Biomed Res Int ; 2014: 349074, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24977149

RESUMO

The possible protective role of ethanolic extract of A. indica tuber (EEAIT) in hepatotoxicity and apoptosis of liver caused by alcohol in rats was investigated. Treatment of rats with alcohol (3 g ethanol per kg body weight per day for 15 days intraperitoneally) produced marked elevation of liver biomarkers such as serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyl transpeptidase (γ-GT), and total bilirubin levels which were reduced by EEAIT in a dose-dependent manner. Furthermore, EEAIT improved antioxidant status (MDA, NO, and GSH) and preserved hepatic cell architecture. Simultaneous supplementation with EEAIT significantly restored hepatic catalase (CAT) and superoxide dismutase (SOD) activity levels towards normal. The studies with biochemical markers were strongly supported by the histopathological evaluation of the liver tissue. EEAIT also attenuated apoptosis and necrosis features of liver cell found in immunohistochemical evaluation. HPLC analysis of the extract showed the presence of three major peaks of which peak 2 (RT: 33.33 min) contains the highest area (%) and UV spectrum analysis identified it as flavonoids. It is therefore suggested that EEAIT can provide a definite protective effect against chronic hepatic injury caused by alcohol in rats, which may mainly be associated with its antioxidative effect.


Assuntos
Alocasia/química , Etanol/efeitos adversos , Falência Hepática/tratamento farmacológico , Fígado/efeitos dos fármacos , Extratos Vegetais/química , Alanina Transaminase/metabolismo , Animais , Antioxidantes/metabolismo , Aspartato Aminotransferases/metabolismo , Bilirrubina/metabolismo , Catalase/metabolismo , Cromatografia Líquida de Alta Pressão , Relação Dose-Resposta a Droga , Feminino , Glutationa/metabolismo , Imuno-Histoquímica , Peroxidação de Lipídeos , Fígado/enzimologia , Falência Hepática/induzido quimicamente , Óxido Nítrico/metabolismo , Tubérculos/química , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismo , gama-Glutamiltransferase/metabolismo
12.
Environ Technol ; 34(13-16): 2221-9, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24350476

RESUMO

Land application of sewage sludge usually leads to increased levels of heavy metals in soil, plants and groundwater. Pre-treatment using plants has been proposed to reduce the contents of heavy metals and water in sludge prior to land application. This study quantified the transfer of Zn, Cd, Pb and major nutrients in a sludge-soil-plant-leachate system during the treatment of sewage sludge. To accomplish this, a two year pot experiment was carried out to collect leachate, mono- and co-cropping of Sedum alfredii and feed crops was conducted in sludge with an under-layer soil support. Sludge phyto-treatment increased Zn and Cd concentrations in the under-layer soil, but not Pb. Specifically, 70%, 70% and 80% of the original Zn, Cd and Pb, respectively, remained in the sludge, while about 40%, 70% and 60% of the original N, P and K remained. Only 3% to 5% of Cd and Zn and < 1% of Pb were transferred into the under-layer soils or leachates, while more than 12% of the N and P were transferred. Co-planting S. alfredii and feed crops led to a significant reduction of heavy metals in leachates when compared with sludge without planting. Overall, sludge leachate is more appropriate than whole sludge for recycling in agriculture since it reduces the chance of heavy metal contamination in the agro-ecosystem; therefore, co-cropping phytotreatment of sludge can be coupled with sludge leachate recycling for crop production and re-collection of the sludge residue for landfilling.


Assuntos
Biodegradação Ambiental , Metais Pesados/análise , Plantas/química , Plantas/metabolismo , Esgotos , Poluentes do Solo/análise , Alocasia/química , Alocasia/metabolismo , Biomassa , Metais Pesados/metabolismo , Sedum/química , Sedum/metabolismo , Solo/química , Poluentes do Solo/metabolismo , Zea mays/química , Zea mays/metabolismo
13.
PLoS One ; 8(9): e75328, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24086508

RESUMO

Chinese herbal medicines have long been used to treat various illnesses by modulating the human immune response. In this study, we investigate the immuno-modulating effect and antitumor activity of Alocasia Cucullata (AC), a Chinese herb traditionally used to treat infection and cancer. We found that the whole water extract of AC roots could significantly attenuate tumor growth in mouse tumor models. The median survival time of the AC-treated mice was 43 days, 16 days longer than that of the control group. Moreover, the AC-treated mice showed substantially higher induction of key antitumor cytokines, such as IL-2, IFN-γ, and TNF-α, indicating that AC may exert antitumor effect by activating antitumor immunity. To further pinpoint the cellular and molecular mechanism of AC, we studied the dose response of a human monocytic cell line, THP-1, to the whole water extract of AC. Treatment of the AC extract significantly induced THP-1 differentiation into macrophage-like cells and the differentiated THP-1 showed expression of specific macrophage surface markers, such as CD11b and CD14, as well as productions of antitumor cytokines, e.g. IFN-γ and TNF-α. Our data thus point to AC as potentially a new, alternative immuno-modulating herbal remedy for anticancer treatment.


Assuntos
Alocasia/química , Antineoplásicos/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Neoplasias/tratamento farmacológico , Fitoterapia/métodos , Extratos Vegetais/farmacologia , Animais , Antineoplásicos/imunologia , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/imunologia , Linhagem Celular , Citocinas/imunologia , Relação Dose-Resposta a Droga , Descoberta de Drogas , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Humanos , Camundongos , Extratos Vegetais/imunologia , Análise de Sobrevida , Água
14.
J Integr Med ; 11(5): 343-51, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24063782

RESUMO

OBJECTIVE: To elucidate potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activities of the ethanol extract of Alocasia indica Schott tuber in different experimental models established in vitro and in vivo. METHODS: In vitro antioxidant activity was evaluated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Phenolic content was estimated by using Folin-Ciocalteu's reagent while reducing ability was measured by ferric reducing power assay. In vivo antidiarrheal studies were carried out in mice, and the activity was evaluated in castor oil and magnesium sulfate-induced diarrhea. Disk diffusion assay was utilized to determine antibacterial activity against a number of pathogenic bacterial strains. Acute toxicity test was carried out to measure the safe doses for the extract. RESULTS: In DPPH radical-scavenging assay, the extract exhibited strong radical-scavenging activity with the 50% inhibitory concentration value of 42.66 µg/mL. Total phenolic content was found to be 542.26 mg gallic acid equivalent per 100 g of dried tuber extract, whereas flavonoid content was found to be 4.30 mg quercetin equivalent/g of dried tuber extract. In reducing power assay, the extract showed strong reducing power in a concentration-dependent manner. The extract significantly (P < 0.01) enhanced the latent period and decreased defecation in both castor oil- and magnesium sulfate-induced diarrhea. The extract also lessened gastrointestinal motility in mice. Potential antibacterial activity was exhibited by the extract against all the tested bacterial strains in disk diffusion assay. The 50% lethal concentration against brine shrimp nauplii was 81.09 µg/mL. CONCLUSION: The results demonstrated that the ethanol extract of A. indica has potential antioxidant, antidiarrheal, cytotoxic, and antibacterial activity.


Assuntos
Alocasia , Extratos Vegetais/farmacologia , Alocasia/química , Animais , Antibacterianos/farmacologia , Antidiarreicos/farmacologia , Antioxidantes/farmacologia , Artemia/efeitos dos fármacos , Feminino , Motilidade Gastrointestinal/efeitos dos fármacos , Masculino , Camundongos , Extratos Vegetais/toxicidade
15.
Chem Pharm Bull (Tokyo) ; 60(5): 670-3, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22689406

RESUMO

Five new indole alkaloids, alocasins A-E (3-7), together with known hyrtiosin B (1) and hyrtiosulawesin (2) were isolated from Alocasia macrorrhiza (L.) SCHOTT; their structures were elucidated on the basis of spectroscopic data. Compounds 1-7 were in vitro tested for cytostatic activity on human throat cancer (Hep-2), human hepatocarcinoma (Hep-G2), and human nasopharyngeal carcinoma epithelial (CNE) cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method; compounds 2, 3, 6 and 7 showed mild antiproliferative activity against Hep-2 and Hep-G2 whereas compounds 2 and 4 showed gentle antiproliferative activity against CNE.


Assuntos
Alocasia/química , Antineoplásicos Fitogênicos/química , Alcaloides Indólicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/toxicidade , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Células Hep G2 , Humanos , Alcaloides Indólicos/isolamento & purificação , Alcaloides Indólicos/toxicidade , Espectroscopia de Ressonância Magnética , Rizoma/química
16.
Indian J Exp Biol ; 47(10): 816-21, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20112809

RESUMO

Oral administration of hydroalcoholic extract of A. indica (250 and 500 mg/kg) effectively inhibited CCl4 and paracetamol induced changes in the serum marker enzymes, cholesterol, serum protein and albumin in a dose-dependent manner as compared to the normal and the standard drug silymarin-treated groups. Hepatic steatosis, fatty infiltration, hydropic degeneration and necrosis observed in CCl4 and paracetamol-treated groups were completely absent in histology of the liver sections of the animals treated with the extracts. The results suggests that the hydroalcoholic extract of leaves of A. indica possess significant potential as hepatoprotective agent.


Assuntos
Alocasia/química , Fígado/efeitos dos fármacos , Extratos Vegetais/farmacologia , Folhas de Planta/química , Acetaminofen/toxicidade , Animais , Etanol/química , Feminino , Testes de Função Hepática , Masculino , Ratos , Ratos Wistar
17.
J Fluoresc ; 17(6): 663-9, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17665291

RESUMO

The value of intrinsic chlorophyll fluorescence polarization, and the intensity in emission spectrum were investigated in leaf segments of Alocasia macrorrhiza under several stress conditions including different temperatures (25-50 degrees C), various concentrations of NaCl (0-250 mM), methyl viologen (MV, 0-25 microM), SDS (0-1.0%) and NaHSO(3) (0-80 microM). Fluorescence emission spectrum of leaves at wavelength regions of 500-800 nm was monitored by excitation at 436 nm. The value of fluorescence polarization (P value), as result of energy transfer and mutual orientation between chlorophyll molecules, was determined by excitation at 436 nm and emission at 685 nm. The results showed that elevated temperature and concentrations of salt (NaCl), photooxidant (MV), surfactant (SDS) and simulated SO(2) (NaHSO(3)) treatments all induced a reduction of fluorescence polarization to various degrees. However, alteration of the fluorescence spectrum and emission intensity of F(685) and F(731) depended on the individual treatment. Increase in temperature and concentration of NaHSO(3) enhanced fluorescence intensity mainly at F(685), while an increase in MV concentration led to a decrease at both F(685) and F(731). On the contrary, NaCl and SDS did not cause remarkable change in fluorescence spectrum. Among different treatments, the negative correlation between polarization and fluorescence intensity was found with NaHSO(3) treatments only. We concluded that P value being measured with intrinsic chlorophyll fluorescence as probe in leaves is a susceptible indicator responding to changes in environmental conditions. The alteration of P value and fluorescence intensity might not always be shown a functional relation pattern. The possible reasons of differed response to various treatments were discussed.


Assuntos
Alocasia/química , Clorofila/química , Alocasia/efeitos dos fármacos , Polarização de Fluorescência , Temperatura Alta , Oxidantes Fotoquímicos/farmacologia , Paraquat/farmacologia , Folhas de Planta/química , Folhas de Planta/efeitos dos fármacos , Cloreto de Sódio/farmacologia , Dodecilsulfato de Sódio/farmacologia , Espectrometria de Fluorescência , Sulfatos/farmacologia , Dióxido de Enxofre/farmacologia , Tensoativos/farmacologia
18.
Clin Exp Ophthalmol ; 34(9): 895-6, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17181626

RESUMO

We report a case of crystalline keratopathy caused by Alocasia macrorrhiza. The diagnosis was made based on the observation of needle-like crystals in the corneal stroma following injury to that eye. The condition resolved in 3 months with the disappearance of the crystals confirmed by follow-up confocal microscopy.


Assuntos
Alocasia/química , Doenças da Córnea/etiologia , Substância Própria/lesões , Ferimentos Oculares Penetrantes/etiologia , Estruturas Vegetais/efeitos adversos , Oxalato de Cálcio/metabolismo , Doenças da Córnea/metabolismo , Doenças da Córnea/patologia , Substância Própria/patologia , Cristalização , Feminino , Seguimentos , Humanos , Microscopia Confocal , Pessoa de Meia-Idade
19.
Arch Pharm Res ; 27(10): 1020-2, 2004 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-15554257

RESUMO

A new ceramide alomacrorrhiza A was isolated from the ethanolic extract of the plant Alocasia macrorrhiza (L.) Schott. Its chemical structure was elucidated as (2S,3S,4R)-2N-[(2'R)-2'-hydroxy-hexacosanoyl]-tetradecane-1,3,4-triol based on extensive 1D, 2D NMR, EI-MS, FAB-MS, HR-FAB-MS spectroscopic data and chemical degradation studies.


Assuntos
Alocasia/química , Ceramidas/química , Acetilação , Hidrólise , Indicadores e Reagentes , Espectroscopia de Ressonância Magnética , Extratos Vegetais/química , Raízes de Plantas/química , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Espectrofotometria Infravermelho , Vietnã
20.
J Environ Sci (China) ; 15(5): 622-7, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-14562922

RESUMO

The production of sewage sludge in China has been increasing sharply in order to treat 40% of the municipal sewage in 2005 as planned by central government. The main sludge disposal method is landfill owing to heavy metal contamination, but it presents an attractive potential for agricultural land application. Experiments were carried out to study the simultaneous metal removal and sludge stabilization by plants. The sludge samples were collected from Datansha Wastewater Treatment Plant of Guangzhou, it contained excessive Cu and Zn compared with the Chinese National Standard for Agricultural Use of Sewage Sludge. Plants growing on sludge beds were investigated to follow their growth and metal uptake. 30 sludge plants were identified during 1 year's observation. A Zn high-accumulating and high growth rate plant(Alocasia macrorrhiza) was selected and grown on sludge beds in plots. The water, organic matter, heavy metals and nutrients contents, the E. coli number and the cress seed germination index were monitored for the sludge samples collected monthly. The plant growth parameters and its heavy metals contents were also determined. The sewage sludge treated by plants could be stabilized at about 5 months, the E. coli number was significantly decreased and the cress seed germination index attained 100%. Crop on sludge could ameliorate the sludge drying. The experiments are continuing to find out the appropriate plant combination for simultaneous sludge stabilization and metal removal for an acceptable period. Comparisons between the proposed processes and other methods for treating produced sludge such as composting, chemical andbacterial leaching were discussed.


Assuntos
Alocasia/crescimento & desenvolvimento , Metais/análise , Esgotos/análise , Poluentes do Solo/análise , Gerenciamento de Resíduos/métodos , Alocasia/química , Biomassa , China , Germinação , Estações do Ano
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