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1.
Presse Med ; 48(12): 1445-1455, 2019 Dec.
Artigo em Francês | MEDLINE | ID: mdl-31761607

RESUMO

The Hormonal assessment of Arterial Hypertension (HTA) is an important part of the balance of resistant hypertension. This assessment - going well beyond the search for primary hyperaldosteronism (PHA) - requires a rigorous methodology and a robust experience of the nursing team within a dedicated unit: the HTA Day Hospital. If all the conditions are met and the assessment carried out well, it will allow a significant profitability since in this resistant hypertensive population it will detect a particular mechanism or secondary hypertension in 70% of patients. Since the diagnosis of PHA is essentially biological, the proper execution of the various stages of the assessment is essential to its documentation.


Assuntos
Técnicas de Diagnóstico Endócrino , Hormônios/análise , Hipertensão/diagnóstico , Aldosterona/análise , Aldosterona/sangue , Anti-Hipertensivos/uso terapêutico , Análise Química do Sangue/métodos , Análise Química do Sangue/normas , Diagnóstico Diferencial , Técnicas de Diagnóstico Endócrino/normas , Resistência a Medicamentos/efeitos dos fármacos , Hormônios/sangue , Humanos , Hiperaldosteronismo/diagnóstico , Hipertensão/sangue , Hipertensão/tratamento farmacológico , Hipertensão/etiologia , Renina/análise , Renina/sangue
2.
Anal Bioanal Chem ; 411(27): 7055-7059, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31598742

RESUMO

A new perspective on the relevant problem-creating simple, rapid, and efficient protein sensors based on microstructured optical fibers using a simple homogeneous analysis format-was proposed. Commercially available long-period grating hollow core microstructured optical fibers (LPG HCMOF) were used to determine bovine serum albumin (BSA) and albumin from chicken eggs (OVA) in binary mixtures as well as immunoglobulin G (IgG) in the presence of BSA and OVA. LPG HCMOF transmission spectra allowed the detection of both BSA and OVA up to 10 mg/mL with LOD as low as 0.1 and 0.8 µg/mL, respectively. Partial least squares regression (PLS) was utilized for modeling of LPG HCMOF spectral data and quantitative analysis of BSA, OVA, total protein, and IgG in binary and ternary mixtures. Rather high coefficients of determination (R2) and low root mean square error for the calibration (RMSEC) (15%) and prediction (RMSEP) (20%) were obtained for all PLS models. The proposed approach was tested in the analysis of BSA in spiked horse blood hemolyzed (HBH). The results demonstrated the functionality of the proposed approach and offered the opportunity for the creation of a wide range of sensors for protein determination in complex mixtures. Graphical abstract.


Assuntos
Imunoglobulina G/análise , Ovalbumina/análise , Soroalbumina Bovina/análise , Animais , Análise Química do Sangue/métodos , Bovinos , Galinhas , Cavalos , Análise dos Mínimos Quadrados , Camundongos , Modelos Moleculares , Fibras Ópticas
3.
Ecotoxicol Environ Saf ; 184: 109609, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31518828

RESUMO

Carbazole and halogenated carbazoles have been widely detected throughout the environment in soil, river deposits, and lake sediments. Human exposure to these compounds may occur through inhalation, drinking water, dietary intake and/or skin contact, and exposure levels in the body may be evaluated by measuring them in serum or blood. This paper reports the method development and validation for the analysis of carbazole and 11 halogenated carbazoles in human blood and/or serum samples. A small sample size of 100 µL of blood or serum was employed for the analysis. The samples were prepared through salting-out liquid-liquid extraction (LLE) by using hexane/ethyl acetate (4:1, v/v) as the extraction solvent and aqueous MgSO4 (37.5 wt%) as the salting-out regent, respectively. Sample analysis was performed using gas-chromatography (GC) coupled with a tandem mass spectrometer (MS/MS) in an electron impact (EI) mode. The developed method demonstrated low detection limits in the range of 0.02-0.27 ng/mL, intra-day accuracy ranging from 81.2% to 125%, and inter-day accuracy from 91.0% to 117%. The intra- and inter-day precisions, calculated by relative standard deviations (RSDs), were in the ranges of 1.0-16.0% and 1.8-16.4%, respectively. The developed method was applied to the analysis of 50 human serum samples collected from pregnant women in Southern California in 2012. Low concentrations of carbazole were measured in 18 samples, while halogenated carbazoles were not detected in any of the samples.


Assuntos
Análise Química do Sangue/métodos , Carbazóis/sangue , Exposição Ambiental/análise , Cromatografia Gasosa-Espectrometria de Massas , Espectrometria de Massas em Tandem , California , Feminino , Halogenação , Humanos , Limite de Detecção , Gravidez
4.
PLoS Negl Trop Dis ; 13(9): e0007669, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31498786

RESUMO

BACKGROUND: Identification of blood sources of hematophagous arthropods is crucial for understanding the transmission cycles of vector-borne diseases. Many different approaches towards host determination were proposed, including precipitin test, ELISA, DNA- and mass spectrometry-based methods; yet all face certain complications and limitations, mostly related to blood degradation. This study presents a novel method for blood meal identification, peptide mass mapping (PMM) analysis of host-specific hemoglobin peptides using MALDI-TOF mass spectrometry. METHODOLOGY/PRINCIPAL FINDINGS: To identify blood meal source, proteins from abdomens of engorged sand fly females were extracted, cleaved by trypsin and peptide fragments of host hemoglobin were sequenced using MALDI-TOF MS. The method provided correct host identification of 100% experimentally fed sand flies until 36h post blood meal (PBM) and for 80% samples even 48h PBM. In females fed on two hosts, both blood meal sources were correctly assigned for 60% of specimens until 36h PBM. In a validation study on field-collected females, the method yielded unambiguous host determination for 96% of specimens. The suitability of PMM-based MALDI-TOF MS was proven experimentally also on lab-reared Culex mosquitoes. CONCLUSIONS/SIGNIFICANCE: PMM-based MALDI-TOF MS analysis targeting host specific hemoglobin peptides represents a sensitive and cost-effective method with a fast and simple preparation protocol. As demonstrated here on phlebotomine sand flies and mosquitoes, it allows reliable and rapid blood source determination even 48h PBM with minimal material input and provides more robust and specific results than other currently used methods. This approach was also successfully tested on field-caught engorged females and proved to be a promising useful tool for large-scale screening of host preferences studies. Unlike other methods including MALDI-TOF protein profiling, it allows correct identification of mixed blood meals as was demonstrated on both experimentally fed and field-collected sand flies.


Assuntos
Análise Química do Sangue/métodos , Psychodidae/fisiologia , Animais , Comportamento Alimentar , Hemoglobinas/química , Humanos , Peptídeos/química , Estudo de Prova de Conceito , Psychodidae/química , Especificidade da Espécie , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Espectrometria de Massas em Tandem
5.
Bioanalysis ; 11(15): 1419-1435, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31490107

RESUMO

Aim: To develop a bioanalytical method to support pharmacokinetic evaluation of DNDI-VL-2098 in mouse, rat, dog and hamster following oral administration. Results & methodology: A robust LC-MS/MS bioanalytical method was developed to quantify DNDI-VL-2098. DNDI-VL-2098 showed time-dependent recovery loss in acetonitrile precipitated plasma in all species. Acid-lysed whole blood was identified as a matrix in which recovery was stable over time. A two-step extraction procedure was used, with protein precipitation followed by liquid-liquid extraction with methyl tert-butyl ether. The assay was validated in the dynamic range of 5-5000 ng/ml for mouse, rat and dog blood, and a fit-for-purpose method was developed for hamster. Conclusion: A specific LC-MS/MS assay for DNDI-VL-2098 was developed and validated in hemolyzed blood.


Assuntos
Análise Química do Sangue/métodos , Cromatografia Líquida/métodos , Nitroimidazóis/sangue , Oxazóis/sangue , Espectrometria de Massas em Tandem/métodos , Métodos Analíticos de Preparação de Amostras , Animais , Cricetinae , Marcação por Isótopo , Camundongos , Ratos
6.
Bioanalysis ; 11(15): 1405-1418, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31401845

RESUMO

Background: Neurofilament light (NfL) chain is an established cerebrospinal fluid (CSF) biomarker for neuroaxonal injury. The highly sensitive Quanterix Simoa™ platform is evaluated for NfL measurement in both CSF and blood. There is a need to link historical ELISA data that use bovine NfL to that of Simoa using a recombinant human (rhuman) NfL standard. Results/Methodology: The Simoa NF-light® Advantage Kit was validated for CSF and qualified for serum and plasma, using both rhuman and bovine NfL calibrators. Matched CSF, serum and plasma samples from 112 multiple sclerosis patients were analyzed using both calibrators. Conclusion: In multiple sclerosis, there is a good correlation between blood and CSF NfL levels. A conversion factor of approximately 5:1 was established between bovine and rhuman NfL calibrators.


Assuntos
Análise Química do Sangue/métodos , Proteínas de Neurofilamentos/sangue , Proteínas de Neurofilamentos/líquido cefalorraquidiano , Animais , Análise Química do Sangue/normas , Calibragem , Bovinos , Humanos , Limite de Detecção , Proteínas de Neurofilamentos/química , Recidiva , Padrões de Referência
7.
J Clin Neurosci ; 69: 190-197, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31409546

RESUMO

We aimed to investigate the correlations between biochemical and hematological markers and the clinical conditions of brain tumor patients before and after craniotomy. A retrospective study was conducted in 90 brain tumor patients. Age, gender, underlying diseases, tumor size and intraoperative blood loss were recorded. Red blood cell counts and hepatic and renal markers were analyzed preoperatively and postoperatively. Albumin decreased by 5.6 g/L after surgery (p < 0.001). Older patients (>52 years) and females had lower albumin levels than younger patients and males did. Red blood cell counts and hemoglobin levels decreased significantly on the 1st and increased on the 3rd postoperative day. The blood glucose level increased on the 1st postoperative day and then decreased. Older patients had higher blood glucose levels than younger patients did (p < 0.05). The postoperative serum sodium, potassium and calcium levels were within the normal ranges; 37 patients had hypocalcemia (41.1%) and patients with hypokalemia and hyponatremia increased postoperatively. Albumin and hemoglobin levels were linearly correlated (correlation coefficient 0.559, p < 0.001). Intraoperative blood loss was correlated with tumor size (p < 0.05) but did not affect the decrease in hematological markers. In brain tumor patients, red blood cell counts and hemoglobin and serum albumin levels were significantly decreased after craniotomy; these effects were influenced by gender and age instead of intraoperative blood loss. The postoperative blood glucose level peaked and then decreased; it was affected by age and diabetes mellitus. Electrolytes remained relatively stable. These findings have implications for patient management and postoperative complication prevention.


Assuntos
Biomarcadores/sangue , Neoplasias Encefálicas/sangue , Neoplasias Encefálicas/cirurgia , Craniotomia , Complicações Pós-Operatórias/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Contagem de Células Sanguíneas , Análise Química do Sangue/métodos , Estudos de Coortes , Craniotomia/efeitos adversos , Feminino , Hemoglobinas , Humanos , Masculino , Pessoa de Meia-Idade , Período Perioperatório , Estudos Retrospectivos
8.
J Chromatogr A ; 1608: 460411, 2019 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-31405572

RESUMO

In the current study, for the first time, the sugaring-out effect was assessed in a conventional electromembrane extraction (EME) system. Utilizing the sugars in the donor solution as green additives can result in production of a pioneering and influential EME mode. Sugaring-out assisted electromembrane extraction was combined with high-performance liquid chromatography (HPLC) to enhance the extraction of four basic model drugs (pseudoephedrine, lidocaine, propranolol, and ketoconazole). In this mode of EME, not only the transfer of analytes through the supported liquid membrane (SLM) was improved, but also the whole extraction system became more stable than the conventional one in the same voltage. The type and concentration of sugars were optimized in addition to the common experimental parameters influencing the EME, and figures of merit were also studied. Under the optimum conditions, repeatability (RSD%) was obtained in the range of 2.8-6.9% in the water, while RSD value was obtained in the range of 8.2-11.8% (n = 3) for conventional EME with the same state. The linearity range was also in the interval of 5.0-1000.0 ng mL-1 and limits of quantification and detection were in the ranges of 5.0-10.0 ng mL-1 and 1.5-3.0 ng mL-1, respectively, in the introduced EME. Extraction recoveries in the range of 41.2 and 80.8% were obtained resulting in enrichment factors in the range of 96-189. In light of such factor, new suggested EME mode was assessed in the real biological samples including human plasma and urine in order to prove the sugaring-out efficiency in EME systems.


Assuntos
Análise Química do Sangue/métodos , Preparações Farmacêuticas/isolamento & purificação , Açúcares/química , Urinálise/métodos , Cromatografia Líquida de Alta Pressão , Eletrônica , Humanos , Membranas Artificiais , Preparações Farmacêuticas/sangue , Preparações Farmacêuticas/urina
9.
Ann Biol Clin (Paris) ; 77(4): 375-380, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31418698

RESUMO

Blood concentration of cystatin C is independent of muscle mass and tubular secretion. It can be used, in the absence of a reference method, as an alternative marker to creatinine for the evaluation of renal function and the estimation of glomerular filtration rate (GFR). Both particle-enhanced immunonephelemetry (PENIA) or immunoturbidimetry (PETIA) methods are available to determine cystatin C. From an analytical point of view, it is recommended to use methods whose calibration is traceable to the reference material (ERM-DA471/IFCC) and to report an estimated GFR based on cystatin C. The main equations used are those developed in 2012 by the group "Chronic kidney disease epidemiology collaboration (CKD-EPI)" for adults and those published by Schwartz in 2012 for children. National and international recommendations suggest using a cystatin C-based GFR estimate as a confirmatory test in the clinical settings where the relationship between creatinine production and muscular mass impairs the clinical performance of creatinine. The indications retained by the working group were graded according to the level of recommendations. The essential indications are the estimation and/or the monitoring of renal function in children and adolescents due to rapid changes in muscle mass; in patients with impaired muscle mass and in patients with an alteration of tubular secretion of creatinine (essentially iatrogenic effects).


Assuntos
Cistatina C/análise , Taxa de Filtração Glomerular , Testes de Função Renal/métodos , Lesão Renal Aguda/sangue , Lesão Renal Aguda/diagnóstico , Adulto , Biomarcadores/análise , Biomarcadores/sangue , Análise Química do Sangue/métodos , Análise Química do Sangue/normas , Criança , Creatinina/sangue , Cistatina C/sangue , Humanos , Testes de Função Renal/normas , Padrões de Referência , Insuficiência Renal Crônica/sangue , Insuficiência Renal Crônica/diagnóstico , Estatística como Assunto/métodos , Estatística como Assunto/normas
10.
Ann Biol Clin (Paris) ; 77(4): 439-445, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31418706

RESUMO

A 67 years old woman with a Waldenström disease was admitted in the intensive care unit for dyspnea and fever. During hospitalization, episodes of undetectable glycemia were observed without any hypoglycemia symptoms. Plasma glucose was determined with the hexokinase method (recommended). From this observation, a literature review on PubMed was performed to investigate similar cases. In patients with protides in excess (e.g. immunoproliferative syndrome), absorption measurements could be disrupted by the precipitation of excess protein (IgM in most cases). Other parameters could be affected: bilirubin, phosphate, HDL cholesterol, GGT, CRP and calcemia. In our case, the main difficulty was to identify the cause of the interference and then correct it. Using a series of dilution, we prevented protide precipitation allowing correct glucose determination. Those interferences are rare, but present a real analytical difficulty. Biologists should be aware of those interferences because of dramatics consequences.


Assuntos
Análise Química do Sangue/métodos , Glicemia/análise , Hexoquinase/metabolismo , Hipoglicemia/diagnóstico , Paraproteínas/efeitos adversos , Macroglobulinemia de Waldenstrom/sangue , Idoso , Artefatos , Análise Química do Sangue/normas , Glicemia/metabolismo , Diagnóstico Diferencial , Dispneia/sangue , Dispneia/diagnóstico , Dispneia/etiologia , Reações Falso-Positivas , Feminino , Febre/sangue , Febre/diagnóstico , Febre/etiologia , Hexoquinase/química , Humanos , Hipoglicemia/sangue , Paraproteínas/metabolismo , Hemorragia Retiniana/sangue , Hemorragia Retiniana/diagnóstico , Hemorragia Retiniana/etiologia , Macroglobulinemia de Waldenstrom/complicações , Macroglobulinemia de Waldenstrom/diagnóstico
12.
Bioanalysis ; 11(14): 1347-1358, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31393160

RESUMO

Aim: The reliable measurement of receptor occupancy (RO) provides informative data for efficacy and safety evaluation. This study aimed to assess factors affecting RO measurement of anti-PD-1 antibodies in clinical studies. Materials & methods: RO performance was assessed using different T-cell activation markers measured by flow cytometry. The validated methodology was then used in support of a clinical study. Results: The optimized active cell population was comprised of CD45RO+ or CD45RA- T cells. The bioanalytical method was validated for inter- and intra-assay precision (coefficient of variation ≤30%) and sample storage stability for 3 days. Consistent RO saturation was observed in Phase Ia clinical trial, although receptor regulation appeared to be different. The formation of anti-drug antibodies had markedly influenced pharmacokinetics and RO. Conclusion: RO measurement in combination with pharmacokinetics and anti-drug antibodies data could allow the integrated evaluation and better understanding of efficacy and safety.


Assuntos
Anticorpos Monoclonais/sangue , Anticorpos Monoclonais/imunologia , Análise Química do Sangue/métodos , Ensaios Clínicos como Assunto , Receptor de Morte Celular Programada 1/imunologia , Calibragem , Humanos , Reprodutibilidade dos Testes
13.
Malar J ; 18(1): 262, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31366365

RESUMO

BACKGROUND: The Mindray BC-6800 haematology analyzer (BC-6800) provides a dedicated flag 'Infected RBC' (InR) and the number of InR (InR#)/the permillage of InR (InR‰) in routine blood testing as a screening tool for malaria in endemic areas. This study sought to evaluate the effectiveness of the BC-6800 flag parameter for aiding the diagnosis of malaria. METHODS: A total of 181 samples were tested using the Mindray BC-6800 haematology analyzer, including 117 malaria-infected samples collected from Yunnan, China, and 64 samples from healthy controls. Microscopy examination was conducted as reference when stained thick blood film revealed the presence of malaria parasites identified as Plasmodium vivax and Plasmodium falciparum. The receiver operating characteristic (ROC) curve analysis was developed using Analyse-it v4.92.3. The Kappa value was determined to evaluate the agreement between BC-6800 and light microscopy. RESULTS: The sensitivity of InR‰ generated by BC-6800 for P. vivax and P. falciparum was 88.3 and 24.1%, respectively; specificity of InR‰ for malaria parasites was 84.3 and 84.3%, respectively; positive predictive value and negative predictive value was 89.4 and 82.7% for P. vivax, and 52.8 and 60.3% for P. falciparum. There was a strong correlation between ΔWBC and InR‰ (R2 = 0.9731 for P. vivax and R2 = 0.9757 for P. falciparum). There was also a significant correlation between parasitaemia and InR# in P. vivax-infected samples (R2 = 0.734). InR# was evaluated using ROC curve analysis, the area under the ROC curve is 0.95 with a 95% confidence interval of 0.926 to 0.974, and the cut-off value is 0.01 × 109/L for P. vivax. However, the ring stage and the early trophozoite stage of Plasmodium cannot be detected easily on BC-6800, possibly because of the small size and low nucleic acid content of these stages. CONCLUSIONS: The findings suggest that the flag 'InR' and the parameters 'InR#/InR‰' provided by the BC-6800 haematology analyzer could be used to screen for malaria in a clinical setting.


Assuntos
Análise Química do Sangue/métodos , Sangue/parasitologia , Hematologia/métodos , Malária Falciparum/diagnóstico , Malária Vivax/diagnóstico , Plasmodium falciparum/isolamento & purificação , Plasmodium vivax/isolamento & purificação , Adolescente , Adulto , Idoso , Análise Química do Sangue/instrumentação , Criança , Pré-Escolar , China/epidemiologia , Feminino , Hematologia/instrumentação , Humanos , Malária Falciparum/epidemiologia , Malária Falciparum/parasitologia , Malária Vivax/epidemiologia , Malária Vivax/parasitologia , Masculino , Pessoa de Meia-Idade , Parasitemia/diagnóstico , Parasitemia/epidemiologia , Parasitemia/parasitologia , Prevalência , Curva ROC , Sensibilidade e Especificidade
14.
Bioanalysis ; 11(14): 1321-1336, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31368774

RESUMO

Aim: Assessment of pharmacokinetic interaction between linagliptin (LNG) and tadalafil (TDL) in healthy males. Methods: First, a novel LC-MS method was developed; second, a Phase IV, open-label, cross-over study was performed. Volunteers took single 20-mg TDL dose on day 1 followed by wash out period of 2 weeks then multiple oral dosing of 5-mg/day LNG for 13 days. On day 13, volunteers were co-administered 20-mg TDL. Results: LNG and TDL single doses did not affect QTc interval. Smoking did not alter pharmacokinetics/pharmacodynamics of LNG and TDL. Co-administration of LNG with TDL resulted in TDL longer time to reach maximum plasma concentration (Tmax), decreased oral clearance (Cl/F) and oral volume of distribution (Vd/F), increased its maximum plasma concentration (Cmax), area under concentration-time curve (AUC), muscle pain and QTc prolongation. Conclusion: LNG and TDL co-administration warrants monitoring and/or TDL dose adjustment.


Assuntos
Análise Química do Sangue/métodos , Cromatografia Líquida/métodos , Voluntários Saudáveis , Linagliptina/farmacocinética , Espectrometria de Massas/métodos , Tadalafila/farmacocinética , Adulto , Métodos Analíticos de Preparação de Amostras , Interações de Medicamentos , Egito , Humanos , Limite de Detecção , Linagliptina/sangue , Masculino , Pessoa de Meia-Idade , Reprodutibilidade dos Testes , Tadalafila/sangue
15.
Ann Biol Clin (Paris) ; 77(4): 447-452, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31339492

RESUMO

The complement system is composed of a set of plasma or membrane proteins. Complement protein deficiencies can be inherited or acquired, through the presence of autoantibodies or through consumption. We evaluated the analytical performance of the Optilite® analyser for the determination of the C3 and C4 levels and for the evaluation of the total complement activity. The intra- and inter-series CVs were evaluated and have showed satisfactory results, the concordances with analysers currently used in the laboratory (BNII® and BCT®, Siemens) are very good, as is the agreement between the serum and plasma samples. We also determined the reference values for the different parameters tested in view of a routine use of Optilite® analyser in the laboratory.


Assuntos
Complemento C3/análise , Complemento C4/análise , Proteínas do Sistema Complemento/análise , Testes Hematológicos/instrumentação , Adulto , Artefatos , Automação Laboratorial/instrumentação , Automação Laboratorial/normas , Análise Química do Sangue/instrumentação , Análise Química do Sangue/métodos , Análise Química do Sangue/normas , Coagulação Sanguínea/fisiologia , Complemento C3/metabolismo , Complemento C4/metabolismo , Proteínas do Sistema Complemento/metabolismo , Contaminação de Equipamentos , Testes Hematológicos/métodos , Testes Hematológicos/normas , Humanos , Padrões de Referência , Reprodutibilidade dos Testes
16.
J Chromatogr A ; 1605: 360351, 2019 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-31307791

RESUMO

In the present work we analyzed the hydrophobicity and hydrophilicity properties of several non-steroidal anti-inflammatory drugs (NSAIDs) by investigating the structural changes of the dynamic hydrogen bond network in order to predict the extraction recovery of NSAIDs from biological fluids set by solid phase extraction (SPE). This work allows investigating the relationship between theoretical descriptors and experimental data using a parameter free method with a strong correlation (Pearson correlation 0.95, p-value 0.0003). The identification and quantification of analytes in human plasma were carried out by high performance liquid chromatography coupled with photodiode array detection (HPLC-PDA) using a Kinetex Evo C18 (150 x 4.6 mm I.D) protected by a guard column and a mixture of acetonitrile and 10 mM phosphate buffer (pH 2.5) (50:50, v/v) as mobile phase at isocratic conditions. Accuracy (BIAS%) ranged within -2.33% and + 8.05% while precision (RSD%) was less than 5.73%.The mean extraction recovery of the carprofen (IS) was 84.1% and the recovery of NSAIDs from human plasma ranged between 81.9% to 86.6%. LODs and LOQs for all the investigated NSAIDs were 0.003 and 0.01 µg/mL, respectively. The method was validated according to the ICH guide line in the range 0.010-20.0 µg/mL.


Assuntos
Anti-Inflamatórios não Esteroides/química , Análise Química do Sangue/métodos , Cromatografia de Fase Reversa , Extração em Fase Sólida , Anti-Inflamatórios não Esteroides/sangue , Cromatografia Líquida de Alta Pressão , Humanos , Limite de Detecção , Plasma/química , Reprodutibilidade dos Testes
17.
Int J Biol Macromol ; 138: 156-167, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31302124

RESUMO

Protein-nanoparticle (NP) interaction, which inevitably form protein corona (PC), has been the subject of much debate about its role in modern biomedical research. In this regard, PC associated with two different NPs viz., magneto-fluorescent (MF) and chitosan coated MF (CMF) NPs were thoroughly investigated, to analyze the effect of polymer coating on protein adsorption. Bradford assay, along with the spectroscopic and microscopic studies suggested increase in adsorbed protein quantity, though the results varied significantly on moving from bare to polymeric coating and in vitro to ex vivo conditions. Interestingly, polymer coated NPs showed increased protein adsorption and induce minimal changes in protein structural integrity under the same conditions. We predict that the changes in secondary structure of primary corona determine the overall signature of surface binding proteins in PC. Our findings suggested that rapid diagnosis of immunoglobulins is possible using the concept of protein corona formation ex vivo.


Assuntos
Análise Química do Sangue/métodos , Imunoglobulinas/sangue , Imunoglobulinas/química , Nanopartículas/química , Proteômica , Adsorção , Quitosana/química , Humanos , Imunoglobulinas/metabolismo , Imãs/química , Teste de Materiais , Estrutura Secundária de Proteína , Propriedades de Superfície , Fatores de Tempo
18.
Talanta ; 204: 379-385, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357309

RESUMO

Fabricating simple, accurate and user-friendly diagnostic device for "point of care testing" (POCT) applications is one of the most challenging objectives in the analytical field. Hemin detection is important for drugs monitoring, diagnosis, and forensic latent bloodstain imaging. Herein is developed, luminol chemiluminescence biosensor for hemin detection using artesunate as coreactant. A possible mechanism to account for the chemiluminescence reaction is discussed. Hemin was detected using both photomultiplier tube (PMT) and smartphone as detector. The detection limit for hemin using smartphone as detector is 20 nM, enabling the visual detection of hemin in blood sample with a dilution factor of blood up to 120,000. While PMT detector is used, the system is able to detect hemin down to 0.22 nM. In addition to high sensitivity, this sensing system exhibit high selectivity. It can successfully distinguish bloodstain from other stains while applying the system for point of care testing using smart phone as detector. Moreover, the system can detect artesunate with a linear range from 0.1 nM to 1.0 µM with a limit of detection of 0.078 nM.


Assuntos
Artesunato/química , Hemina/análise , Substâncias Luminescentes/química , Luminol/química , Artesunato/análise , Análise Química do Sangue/instrumentação , Análise Química do Sangue/métodos , Manchas de Sangue , Calibragem , Humanos , Limite de Detecção , Medições Luminescentes/instrumentação , Medições Luminescentes/métodos , Smartphone
19.
Analyst ; 144(17): 5223-5231, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31360940

RESUMO

Reagent sensors in diagnostic assays are used in medical laboratories to obtain patient results. However, interference during the analysis of blood samples is a constant problem with reagent sensors and leads to inaccurate results. Interference in blood analysis is frequently caused by hemolysis and icterus. This study analyzed the effects of interferents on reagent sensors and devised a method to improve the measurement accuracy using an interference index detection (IID) system to minimize the interference effect. The IID system can be easily applied using only two wells and an optical component for sample measurement. After applying the IID system, the interference rates from bilirubin and hemoglobin improved dramatically. A comparison of results obtained for clinical samples showed that the IID system had a positive effect on the accuracy.


Assuntos
Técnicas Biossensoriais/métodos , Análise Química do Sangue/métodos , Confiabilidade dos Dados , Hemólise , Icterícia , Bilirrubina/sangue , Bilirrubina/química , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/estatística & dados numéricos , Análise Química do Sangue/instrumentação , Análise Química do Sangue/estatística & dados numéricos , Nitrogênio da Ureia Sanguínea , Cloretos/sangue , Hemoglobinas/análise , Hemoglobinas/química , Humanos , Análise de Regressão , gama-Glutamiltransferase/sangue
20.
Talanta ; 204: 826-832, 2019 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-31357371

RESUMO

The importance of blood serum testing in most medical diagnosis has led researchers to seek for a reliable analysis method. Raman spectroscopy is a potential tool for probing serum components due to its real-time and non-destructive measurements without need to any additional reagents. So, Raman spectroscopy for the analysis and discrimination of human serum of healthy and gastric cancer subjects is investigated in this work. In order to find the correlation between Raman spectra and enzymatic test results of glucose, cholesterol, HDL, LDL and triglycerides of serum samples, the partial least squares regression (PLSR) method is utilized. Moreover, the Raman spectroscopy is used to distinguish between serum samples of healthy people and gastric cancer patients using partial least square discriminant analysis (PLS-DA) method. Correlation results reveal that for all serum components, the correlation coefficients between Raman spectra and all enzymatic test results are above 94% significantly. Discrimination results of healthy subjects and gastric cancer patients show that 87.5 ±â€¯2.5% of healthy and gastric cancer subjects are diagnosed properly. Our preliminary results can confirm the Raman method in analysis of serum and also as a diagnostic tool in screening of gastric cancer.


Assuntos
Soro/química , Neoplasias Gástricas/diagnóstico , Adulto , Análise Química do Sangue/métodos , Glicemia/análise , Colesterol/sangue , Análise Discriminante , Feminino , Humanos , Lipoproteínas HDL/sangue , Lipoproteínas LDL/sangue , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Análise Espectral Raman/métodos , Neoplasias Gástricas/sangue , Triglicerídeos/sangue
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