Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 2.165
Filtrar
1.
J Zoo Wildl Med ; 51(4): 915-925, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33480572

RESUMO

Clinical health checks including blood testing before brumation in fall are an important tool in reptile medicine and help to reduce the risk of complications during brumation. Electrophoresis may be useful for the evaluation of liver cell function and the detection of antigenic stimulation. The goal of this study was to compare species-specific reference intervals for plasma chemistry analytes and capillary zone electrophoresis (CZE) for a variety of pet chelonian species in fall. Lithium heparinized samples were collected from 68 clinically healthy spur-thighed tortoises (Testudo graeca), 79 red-eared sliders (Trachemys scripta elegans), and 30 map turtles (Graptemys spp.) from September to November 2016 and 2017. During the same period, 128 equivalent samples were collected from Hermann's tortoises (Testudo hermanni) and the obtained data was used for comparison. Chemistry analytes were measured from plasma using an autoanalyzer and electrophoresis was carried out by CZE. Significant variations (P < 0.0001) between the species were found for several analytes including total protein, percent albumin, and albumin: globulin ratio, α-, ß-, and γ-globulin, alkaline phosphatase, glutamate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, bile acids, creatine kinase, urea, uric acid, inorganic phosphorus, total calcium, and potassium. The variations in albumin (g/l) measured by CZE were also significant (P = 0.0064). No significant variations were detected for sodium levels. The results demonstrate the importance of species-specific reference intervals and provide reference intervals for the plasma chemistry and capillary zone electrophoresis in three chelonian species.


Assuntos
Análise Química do Sangue/veterinária , Eletroforese Capilar/veterinária , Tartarugas/sangue , Animais , Análise Química do Sangue/métodos , Feminino , Masculino , Valores de Referência , Estações do Ano , Especificidade da Espécie
2.
Bull Environ Contam Toxicol ; 105(4): 582-587, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32948914

RESUMO

Oreochromis niloticus was exposed to 10.0 ppb of organophosphate insecticide chlorpyrifos (CPF) and avermectin insecticides abamectin (ABM) and emamectin benzoate (EB) for 48 and 96 h. RBC and Hb decreased in CPF- and ABM-exposed fish after 96-h. Plasma ALT, AST, cortisol, and glucose increased in 96-h CPF-, ABM- and EB-exposed fish, while plasma ions declined in 96-h CPF-exposed ones. Insecticides caused alterations in liver oxidative stress parameters. In fish exposed to CPF, CAT increased after 48-h whereas it decreased after 96-h. Also, CAT declined in 48- and 96-h ABM-exposed fish, whereas it elevated in 48-h EB-exposed ones. Insecticides caused decreases in SOD at 48- and 96-h and in GR after 96-h. GSH elevated in CPF-exposed fish after 48-h, while it decreased in all the tested insecticide exposures after 96-h. Malondialdehyde of fish exposed to insecticides for 96-h increased. Consequently, toxic effects of insecticides on O. niloticus were generally as CPF > ABM > EB.


Assuntos
Clorpirifos/toxicidade , Ciclídeos , Inseticidas/toxicidade , Ivermectina/análogos & derivados , Poluentes Químicos da Água/toxicidade , Animais , Análise Química do Sangue/veterinária , Testes Hematológicos/veterinária , Ivermectina/toxicidade , Fígado/efeitos dos fármacos , Fígado/enzimologia , Oxirredução , Distribuição Aleatória , Testes de Toxicidade Aguda/veterinária
3.
Anim Genet ; 51(4): 601-606, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32511786

RESUMO

We report haplotype-based GWASs for 33 blood parameters measured in 843 Italian Large White pigs. In the single-trait analysis, a total of 30 QTL for number of basophils, six erythrocyte traits (haemoglobin, haematocrit, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, mean corpuscular volume and red blood cell count) and two clinical-biochemical traits (alkaline phosphatase and Ca2+ contents) were identified. In the multiple-trait analysis, a total of five QTL affected three different clusters of traits. Only four of these QTL were already reported in the single-marker and multi-marker GWASs we previously carried out on the same pig population. QTL on SSC11 and SSC17 showed effects on multiple traits. These results further dissected the genetic architecture of parameters that could be used as proxies in breeding programmes for more complex traits. In addition, these results might help to better define the pig as an animal model for several blood-related biological functions.


Assuntos
Análise Química do Sangue/veterinária , Estudo de Associação Genômica Ampla/veterinária , Haplótipos , Animais , Itália , Masculino , Sus scrofa
4.
J Zoo Wildl Med ; 51(2): 438-442, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32549576

RESUMO

Blood samples collected from captive Southern lechwes (Kobus leche) were used to develop reference intervals for complete blood count and serum biochemistry values. The study population consisted in 108 healthy individuals, including 62 females and 46 males and 38 neonates (<2 days old), 27 juveniles (2 mo to 2 yr old), and 43 adults (>2 yr old) from the Réserve Africaine de Sigean, Sigean, France, between 2014 and 2016. No significant effects of sex and season of sampling were found. No significant differences were found for all hematology and biochemistry parameters between juveniles and adults, and their results were then combined. Significant differences were found between animals >2 mo old and neonates for most tested parameters; in particular, neonates had lower hemoglobin, eosinophil count, alanine transaminase, and total protein values, but higher alkaline phosphatase values. The reference intervals obtained were similar to values previously reported for other Bovidae species.


Assuntos
Antílopes/sangue , Análise Química do Sangue/veterinária , Testes Hematológicos/veterinária , Animais , Animais de Zoológico/sangue , França , Valores de Referência
5.
J Vet Sci ; 21(2): e17, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32233127

RESUMO

Dehydration, electrolyte disturbance, and acid-base imbalance are the most significant consequences of diarrhea in calves. We aimed to determine blood gas, hematological, electrolyte, and biochemical values and investigate the relationship between the physical status and blood parameters in Korean native calves (KNCs) with diarrhea. One hundred eighty KNCs with diarrhea (age < 75 days) were investigated. Blood samples were collected from the external jugular vein and analyzed using a portable clinical blood gas analyzer. The measured parameters were statistically compared according to the status of physical activity, dehydration, or prognosis. The mean values of parameters in the Calves with diarrhea showed metabolic acidosis, hyponatremia, and azotemia. The mean values of potassium, chloride, hematocrit, and hemoglobin were in the upper limit of their reference ranges. More than 75% of the calves had metabolic acidosis caused by bicarbonate loss, and 63.6% had high blood urea nitrogen (BUN) values. Moreover, BUN showed the highest correlation with the physical activity status and dehydration. pH, base excess of the extracellular fluid (BE), anion gap, potassium, hematocrit, bicarbonate, and hemoglobin were closely correlated with physical deterioration and dehydration (p < 0.001). BUN, pH, BE, and anion gap were closely correlated with physical deterioration and dehydration. These correlations between clinical symptoms and blood gas parameters can be clinically relevant in predicting the status of parameters according to clinical symptoms.


Assuntos
Doenças dos Bovinos , Diarreia/veterinária , Animais , Animais Recém-Nascidos , Análise Química do Sangue/veterinária , Bovinos , Doenças dos Bovinos/sangue , Doenças dos Bovinos/fisiopatologia , Diarreia/sangue , Diarreia/fisiopatologia , Feminino , Testes Hematológicos/veterinária , Masculino , República da Coreia
6.
J Zoo Wildl Med ; 51(1): 25-38, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32212543

RESUMO

The reference intervals of health parameters are valuable tools for veterinarians and conservationists to monitor the health status and viability of endangered species. Natural variation in the health of the long-lived Asian elephant (Elephas maximus) is poorly understood, particularly in relation to differences between males and females. Longitudinal health data were collected from clinical examination, hematology, and serum chemistry analyses over 3 yr from 227 healthy individually marked Asian elephants varying in age and sex. The study population was semicaptive and used in Myanmar's timber industry, but maintained natural feeding and breeding behavior. Body condition score (BCS) and blood pressure were investigated in clinical examinations. Hematological parameters included hematocrit, hemoglobin, total white blood cell count, and differential blood cell counts. Serum chemistry parameters included blood urea nitrogen, creatinine, total protein, albumin, globulins, aspartate aminotransferase, alkaline phosphatase, triglycerides, creatine kinase, glucose, calcium, potassium, sodium, and chloride. To the knowledge of the authors, this is the first description of BCS in an elephant population outside of zoos, and of blood pressure in this species using a novel adaptation of the Intelli Wrap Cuff pressure monitor. Several differences between the sexes were observed, with females generally having higher BCS and triglycerides, and males displaying higher alkaline phosphatase and glucose levels. This study provides important clinical tools that can be used to assess the health status and improve management in this endangered species.


Assuntos
Análise Química do Sangue/veterinária , Elefantes/fisiologia , Testes Hematológicos/veterinária , Animais , Animais de Zoológico/sangue , Animais de Zoológico/fisiologia , Conservação dos Recursos Naturais , Elefantes/sangue , Espécies em Perigo de Extinção , Feminino , Masculino , Mianmar , Valores de Referência , Fatores Sexuais
7.
J Zoo Wildl Med ; 51(1): 228-231, 2020 Mar 17.
Artigo em Inglês | MEDLINE | ID: mdl-32212568

RESUMO

Baseline health parameters are limited in the primary literature for gray seals (Halichoerus grypus) in the northwest Atlantic. Accurate normal physiologic reference ranges for both species and specific geographic populations are vital tools for assessing the health of individuals and understanding the health of the entire population. This study developed comprehensive reference intervals for biochemical and hematologic parameters of recently weaned gray seal pups on Cape Cod, Massachusetts from samples collected in 2013, 2016, and 2017. Reference ranges were developed using methodology outlined by the American Society of Clinical Veterinary Pathology. By establishing more comprehensive biochemical and hematologic reference ranges for this population based on a robust sample size, this study provides a new tool for clinicians, researchers, and rehabilitation organizations to improve individual patient care and population research.


Assuntos
Análise Química do Sangue/veterinária , Testes Hematológicos/veterinária , Focas Verdadeiras/sangue , Animais , Animais Selvagens/sangue , Feminino , Masculino , Massachusetts , Valores de Referência , Desmame
8.
Trop Anim Health Prod ; 52(5): 2243-2250, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32125595

RESUMO

Porcine circovirus2 (PCV2) infection in pigs is one of the major causes of economic loss to the farmers in terms of low production, slow growth and increase post-weaning mortality rate. The effect of PCV2 infection on haemogram, serum biochemical profile and oxidant/anti-oxidant status is not well established in pigs. In the present study, haemogram, serum biochemical profile and oxidant/anti-oxidant status were assessed in pigs confirmed positive for PCV2 infections as evidenced by commercially available enzyme-linked immunosorbent assay kit (n = 151) and polymerase chain reaction (PCR) (n = 42) among a total of 306 number of pigs included in the study. Non-infected healthy pigs (n = 6) served as healthy control. The total erythrocyte count (TEC), haemoglobin (Hb), packed cell volume (PCV), total leukocyte count (TLC), differential leukocyte count (DLC) and thrombocyte count were measured. The levels of total protein, albumin, globulin, total bilirubin, direct bilirubin, blood urea nitrogen (BUN), creatinine and glucose and enzymes viz. alanine transaminase (ALT), aspartate transaminase (AST), gamma-glutamyl transferase (GGT) and alkaline phosphatase (ALP) were measured. Oxidative stress indicators such as plasma malondialdehyde (MDA) and total anti-oxidant activity (TAOA) were measured using commercially available kits. The mean values of TLC, lymphocytes and thrombocyte count were significantly (P < 0.05) low in PCV2-infected pigs. The levels of globulin, AST, GGT, BUN and creatinine were significantly increased (P < 0.05) whereas levels of albumin and glucose significantly (P < 0.05) decreased in PCV2-infected pigs. The significant increase (P < 0.05) in MDA level and significant decrease (P < 0.05) in TAOA level were noticed in PCV2-infected animals as compared with healthy control. The present study supports immunosuppression, possible multiple organ damage and oxidative stress associated with naturally occurring PCV2 infection in pigs. Timely vaccination and managemental practices can reduce PCV2 infection in farms. In spite of many research studies, there is still paucity of detailed systemic study on haemato-biochemical alteration and oxidative stress associated with PCV2 infection.


Assuntos
Antioxidantes/metabolismo , Contagem de Células Sanguíneas/veterinária , Análise Química do Sangue/veterinária , Infecções por Circoviridae/veterinária , Oxidantes/metabolismo , Doenças dos Suínos/fisiopatologia , Animais , Infecções por Circoviridae/sangue , Infecções por Circoviridae/fisiopatologia , Infecções por Circoviridae/virologia , Circovirus/fisiologia , Ensaio de Imunoadsorção Enzimática/veterinária , Feminino , Masculino , Reação em Cadeia da Polimerase/veterinária , Suínos , Doenças dos Suínos/sangue , Doenças dos Suínos/virologia
9.
PLoS One ; 15(3): e0229800, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32126109

RESUMO

Blood glucose is vital for many physiological pathways and can be quantified by clinical chemistry analyzers and in-house point-of-care (POC) devices. Pre-analytical and analytical factors can influence blood glucose measurements. This project aimed to investigate pre-analytical factors on whole blood and plasma glucose measurements in loggerhead sea turtles (Caretta caretta) by evaluating the effects of storage (refrigeration) up to 48h after sampling and of packed cell volume (PCV) on whole blood glucose analysis by POC glucometer (time series n = 13); and by evaluating the effects of storage (room temperature and refrigeration) on plasma glucose concentrations using a dry slide chemistry analyzer (DCA) at various conditions: immediate processing and delayed plasma separation from erythrocytes at 24h and 48h (time series n = 14). The POC glucometer had overall strong agreement with the DCA (CCC = 0.76, r = 0.84, Cb = 0.90), but consistently overestimated glucose concentrations (mean difference: +0.4 mmol/L). The POC glucometer results decreased significantly over time, resulting in a substantial decline within the first 2h (0.41±0.47 mmol/L; 8±9%) that could potentially alter clinical decisions, thereby highlighting the need for immediate analysis using this method. The effects of PCV on glucose could not be assessed, as the statistical significance was associated with one outlier. Storage method significantly affected plasma glucose measurements using DCA, with room temperature samples resulting in rapid decreases of 3.57±0.89 mmol/L (77±9%) over the first 48h, while refrigerated samples provided consistent plasma glucose results over the same time period (decrease of 0.26±0.23 mmol/L; 6±5%). The results from this study provide new insights into optimal blood sample handling and processing for glucose analysis in sea turtles, show the suitability of the POC glucometer as a rapid diagnostic test, and confirm the reliability of plasma glucose measurements using refrigeration. These findings emphasize the need to consider pre-/analytical factors when interpreting blood glucose results from loggerhead sea turtles.


Assuntos
Análise Química do Sangue/veterinária , Glicemia/análise , Plasma/química , Manejo de Espécimes/veterinária , Tartarugas/sangue , Animais , Análise Química do Sangue/instrumentação , Análise Fatorial , Feminino , Valores de Referência , Reprodutibilidade dos Testes , Fatores de Tempo
10.
Res Vet Sci ; 130: 41-47, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32126390

RESUMO

Blood collection at exsanguination is a method of collecting samples at abattoirs which itself has no effect on animal welfare, compared with collection prior to stunning. However, there is the potential for artefact to affect measurements. It was hypothesised that, for most blood analytes measured, the differences between lairage and exsanguination measurements would be minimal, except for creatine kinase, which was expected be higher in exsanguination samples. Fifty-nine male dairy calves approximately 5-10 days old were sampled prior to slaughter, and again during exsanguination after stunning. Each sample was analysed for packed cell volume, serum urea, beta-hydroxybutyrate, gamma-glutamyl transferase, total protein, glucose and creatine kinase concentrations. Exsanguination and lairage blood results were compared using a paired t-test. There were no significant differences between the measurements taken at exsanguination compared with lairage for packed cell volume, urea and beta-hydroxybutyrate. Glucose concentrations were higher in exsanguination samples, and total protein concentrations were lower, but for both of these analytes the differences were clinically small. Gamma-glutamyl transferase activity was lower in exsanguination samples compared with lairage samples. Creatine kinase activity was higher in exsanguination samples. It was concluded that collecting blood at exsanguination is a valid method for collecting samples for measurement of packed cell volume, urea, and beta-hydroxybutyrate in calves. Glucose and total protein can also yield useful measurements in these samples, though care needs to be taken with interpretation given the minor differences between exsanguination and lairage measurements. Exsanguination samples may be unsuitable for creatine kinase and gamma-glutamyl transferase measurement.


Assuntos
Matadouros , Análise Química do Sangue/veterinária , Coleta de Amostras Sanguíneas/veterinária , Creatina Quinase/análise , Animais , Análise Química do Sangue/métodos , Coleta de Amostras Sanguíneas/métodos , Bovinos , Masculino
11.
Can J Vet Res ; 84(1): 67-73, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31949330

RESUMO

This study was designed to evaluate the antioxidative status of serum by measuring its total antioxidant capacity, as well as the antioxidant enzyme activity (superoxide dismutase, catalase, and glutathione reductase), in dogs with various stages of degenerative mitral valve disease (DMVD) compared to healthy controls. In total, 71 client-owned dogs in different stages of DMVD, which included healthy controls, took part in the study. Following an anamnesis, clinical examination, standard transthoracic echocardiograpic examination, chest X-ray, complete blood (cell) count, and serum biochemistry, dogs were divided into 2 study groups. Blood was drawn from each dog once at the time of presentation and selected antioxidant parameters were measured using commercially available assay kits. The activity of superoxide dismutase gradually decreased in the more advanced stages of DMVD, while the activity of catalase was significantly higher in the group of dogs with asymptomatic DMVD compared to healthy controls and dogs with symptomatic DMVD. No significant changes were noted in total antioxidant capacity and the activity of glutathione reductase. Results suggested that DMVD has a significant impact on the activity of superoxide dismutase and catalase in the serum of the tested dogs. Knowledge of changes in the activity of antioxidative enzymes may warrant further studies, possibly to evaluate the potential role of compounds with antioxidative properties in the clinical outcome of dogs with DMVD.


Assuntos
Antioxidantes/metabolismo , Catalase/sangue , Doenças do Cão/enzimologia , Glutationa Redutase/sangue , Insuficiência da Valva Mitral/veterinária , Superóxido Dismutase/sangue , Análise de Variância , Animais , Contagem de Células Sanguíneas/veterinária , Análise Química do Sangue/veterinária , Estudos de Casos e Controles , Doenças do Cão/sangue , Doenças do Cão/diagnóstico , Cães , Ecocardiografia/veterinária , Feminino , Insuficiência Cardíaca/enzimologia , Insuficiência Cardíaca/etiologia , Insuficiência Cardíaca/veterinária , Masculino , Valva Mitral/diagnóstico por imagem , Valva Mitral/patologia , Insuficiência da Valva Mitral/sangue , Insuficiência da Valva Mitral/diagnóstico , Insuficiência da Valva Mitral/enzimologia
12.
Can J Vet Res ; 84(1): 79-81, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31920219

RESUMO

The objective of this study was to evaluate the association between ketonemia and serum paraoxonase-1 (PON1), malondialdehyde (MDA), and other blood components in tail and mammary veins of dairy cows. Forty-two Holstein dairy cows with decreased feed intake were divided into HIGH (≥ 1.2 mM; n = 31) and LOW (< 1.2 mM; n = 11) groups based on the ß-hydroxybutyrate concentration in plasma collected from the tail vein. The HIGH group had a significantly greater plasma non-esterified fatty acid (NEFA) concentration, but significantly lower serum PON1 activity and phospholipid concentration, and a tendency to have a lower cholesterol ester concentration than the LOW group. Serum PON1 activity was not correlated with the MDA concentration but was positively correlated with serum concentrations of cholesterol esters and phospholipids, and negatively correlated with the plasma NEFA concentration. These results suggest that serum PON1 activity is reduced by hyperketonemia and the relevance of PON1 to MDA seems to not be direct, though it is involved.


Assuntos
Arildialquilfosfatase/sangue , Doenças dos Bovinos/enzimologia , Cetose/veterinária , Malondialdeído/sangue , Ácido 3-Hidroxibutírico/sangue , Animais , Análise Química do Sangue/veterinária , Bovinos , Doenças dos Bovinos/sangue , Ésteres do Colesterol/sangue , Colorimetria/veterinária , Ácidos Graxos não Esterificados/sangue , Feminino , Cetose/sangue , Cetose/enzimologia , Glândulas Mamárias Animais/irrigação sanguínea , Fosfolipídeos/sangue , Cauda/irrigação sanguínea
13.
J Zoo Wildl Med ; 50(4): 937-946, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-31926526

RESUMO

Listed as near-threatened by the International Union for Conservation of Nature (IUCN), the southern hairy-nosed wombat (SHNW, Lasiorhinus latifrons) faces threats such as drought, habitat degradation and loss, disease, and persecution because of competition with agriculture. To assist with evaluation of wombat health, this study reports serum biochemical reference intervals (RIs) for wild-caught SHNW from South Australia established from 126 apparently healthy SHNW using a Beckman Coulter AU480® Automated Chemistry Analyzer using RefVal Advisor. Partitioning of RIs for male and female wombats and for the two methods of sampling was performed as appropriate, and additional significant differences (P < 0.05) in biochemical profiles were identified across age class and season examined. A number of differences were observed between male and female wombats, most notably higher creatinine, urea, and sodium in females. Subadult and juvenile wombats had significantly lower total protein, globulin, and ALT activity, and significantly higher ALP activity than adults. Wombats sampled in winter and spring had significantly higher total protein, albumin, potassium, bicarbonate, and enzyme activities (ALP, ALT, AST, GGT, GLDH, lipase), and significantly lower glucose and creatinine when compared to individuals sampled in summer and autumn. Differences in CK activity and anion gap observed between the two methods of sampling likely reflect delay and handling of animals between capture and blood collection. The serum biochemical RIs documented here are considered representative of a population of healthy SHNW, providing a tool for health assessment and monitoring of SHNW health in South Australia and elsewhere.


Assuntos
Marsupiais/sangue , Envelhecimento , Animais , Animais Selvagens , Austrália , Análise Química do Sangue/veterinária , Feminino , Testes Hematológicos/veterinária , Masculino , Valores de Referência , Estações do Ano
14.
Trop Anim Health Prod ; 52(4): 1977-1984, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31981052

RESUMO

Newcastle disease (ND), caused by virulent Avian avulavirus 1 (AAvV 1), affects variety of avian species around the globe. Several AAvV 1 viruses of different genotypes have recently emerged with varying clinical impacts on their susceptible hosts. Although experimental infection with velogenic and mesogenic strains in chickens and pigeons is well-studied, nevertheless, there exists a paucity of data for comparative variations in serum biochemistry profile of susceptible hosts upon challenge with isolates of varying pathogenicities. With this background, a comparative assessment of a range of serum biochemical parameters was made following challenge with duck-originated velogenic strain (sub-genotype VIIi; MF437287) and pigeon-originated mesogenic strain (sub-genotype VIm; KU885949) in chickens and pigeons. For each of the isolate, commercial broiler chickens and wild pigeons were challenged (10-6.51 EID50/0.1 mL for sub-genotype VIIi and 10-6.87 EID50/0.1 mL sub-genotype Vim) separately via intranasal and intraocular route. Sera were collected on 0, 3rd, 5th, 7th, and 9th day post-infection (dpi), and processed for quantitative analysis of different biochemical parameters. By day 3 post-infection (pi), a substantial decrease (p < 0.0001) in serum alkaline phosphatase (ALP) was observed in chickens and pigeons challenged with velogenic isolate. On the other hand, from day 5 pi and onward, a significant increase (p < 0.001) in serum ALP and total protein concentration was observed exclusively in pigeons challenged with mesogenic isolate. For serum aspartate aminotransferase (AST), a significant increase (p < 0.05) in concentration was observed on day 3 pi which decreased from day 5 pi and onward in pigeons and chickens challenged with mesogenic isolate. Also, to reveal antigenic differences among homologous and heterologous vaccine and field-prevalent strains, cross-hemagglutination inhibition assay demonstrated antigenically diverse nature (R-value < 0.5) of both strains from vaccine strain (LaSota, genotype II). The study concludes antigenic differences among prevalent genotypes than vaccine strain and, although requires further studies to ascertain study outcomes, the serum biochemical profile may facilitate presumptive diagnosis of disease in their susceptible hosts.


Assuntos
Doenças das Aves/virologia , Galinhas , Columbidae , Doença de Newcastle/virologia , Vírus da Doença de Newcastle/fisiologia , Animais , Doenças das Aves/sangue , Doenças das Aves/imunologia , Análise Química do Sangue/veterinária , Testes de Inibição da Hemaglutinação/veterinária , Doença de Newcastle/sangue , Vírus da Doença de Newcastle/genética , Doenças das Aves Domésticas/sangue , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/virologia
15.
Vet Clin North Am Equine Pract ; 36(1): 1-14, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31987706

RESUMO

Clinical pathology results are only as good as the quality of samples and accompanying information submitted to the diagnostic laboratory. The frustration of nondiagnostic or equivocal test results can often be avoided by taking the time to follow sample handling and submission guidelines. This article discusses preanalytical errors that commonly affect the accuracy of hematology, chemistry, and cytology testing, and offers practical tips for preventing these errors and maximizing diagnostic yield.


Assuntos
Doenças dos Cavalos/sangue , Doenças dos Cavalos/diagnóstico , Cavalos/sangue , Animais , Biópsia por Agulha Fina/economia , Biópsia por Agulha Fina/veterinária , Análise Química do Sangue/economia , Análise Química do Sangue/veterinária , Técnicas Citológicas/economia , Técnicas Citológicas/veterinária , Hematologia , Doenças dos Cavalos/patologia , Manejo de Espécimes , Estados Unidos
16.
J Therm Biol ; 87: 102457, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31999598

RESUMO

Impact of global warming on the dairy industry has gained attention due to huge economic losses through low production and fertility caused by heat stress. Exposure to hyperthermia provokes a series of complex responses in mammals which are been related to morphological and physiological alterations including the production of reactive oxygen species (ROS). A quantitative spectrophotometric based nitroblue tetrazolium (NBT) reduction assay was used to estimate the superoxide anion (•O2-) level in heat stressed (at 42 °C) whole blood cultures of native and crossbred bulls (Sahiwal and Frieswal), in vitro. The breed effect in the kinetics of •O2- production at different time periods of continual heat stress was analyzed by repeated measures ANOVA. Comparison between different time periods in reference to 37 °C was analyzed by paired t-test. The •O2- level was significantly different (p < 0.05) between cells at 37 °C and 42 °C at different periods of incubation. Kinetics study showed increment of •O2- production on the acute phase of stress followed by a reduction in both Sahiwal and Frieswal breeds. In Sahiwal breed, the inflated superoxide level continued abated till 4 h and raised again at 6 h, while in Frieswal •O2- level reverted to raise sooner with in 2 h of incubation itself. Contrarily, kinetic of •O2- level in plasma showed a significant reduction (p < 0.001) at 30 min of 42 °C incubation followed by increment of •O2- level. Further, the breed variation was significant (p < 0.05) and a significant high reduction of •O2- level was observed in Sahiwal breed. Our finding indicates that, a better and longer •O2- production homeostasis and higher plasma scavenging ability of native breed may be one of the reasons for the higher thermal tolerance of these breeds in tropical climate.


Assuntos
Doenças dos Bovinos/sangue , Bovinos/fisiologia , Transtornos de Estresse por Calor/veterinária , Superóxidos/sangue , Animais , Análise Química do Sangue/métodos , Análise Química do Sangue/veterinária , Bovinos/sangue , Bovinos/genética , Doenças dos Bovinos/genética , Células Cultivadas , Transtornos de Estresse por Calor/sangue , Transtornos de Estresse por Calor/genética , Hibridização Genética , Indicadores e Reagentes , Nitroazul de Tetrazólio , Espectrofotometria/métodos , Espectrofotometria/veterinária
17.
J Vet Sci ; 21(1): e16, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31940695

RESUMO

Cancer is a major cause of death in dogs worldwide, and the incidence of cancer in dogs is increasing. The attenuated total reflection Fourier transform infrared spectroscopic (ATR-FTIR) technique is a powerful tool for the diagnosis of several diseases. This method enables samples to be examined directly without pre-preparation. In this study, we evaluated the diagnostic value of ATR-FTIR for the detection of cancer in dogs. Cancer-bearing dogs (n = 30) diagnosed by pathologists and clinically healthy dogs (n = 40) were enrolled in this study. Peripheral blood was collected for clinicopathological diagnosis. ATR-FTIR spectra were acquired, and principal component analysis was performed on the full wave number spectra (4,000-650 cm-1). The leave-one-out cross validation technique and partial least squares regression analysis were used to predict normal and cancer spectra. Red blood cell counts, hemoglobin levels and white blood cell counts were significantly lower in cancer-bearing dogs than in clinically healthy dogs (p < 0.01, p < 0.01 and p = 0.03, respectively). ATR-FTIR spectra showed significant differences between the clinically healthy and cancer-bearing groups. This finding demonstrates that ATR-FTIR can be applied as a screening technique to distinguish between cancer-bearing dogs and healthy dogs.


Assuntos
Contagem de Células Sanguíneas/veterinária , Análise Química do Sangue/veterinária , Sangue/parasitologia , Doenças do Cão/diagnóstico , Neoplasias/veterinária , Espectroscopia de Infravermelho com Transformada de Fourier/veterinária , Animais , Cães , Feminino , Masculino , Neoplasias/diagnóstico , Espectroscopia de Infravermelho com Transformada de Fourier/instrumentação , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Tailândia
18.
Trop Anim Health Prod ; 52(2): 525-531, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31392552

RESUMO

This study was conducted to evaluate the influence of increased supplementation of zinc oxide (ZnO) on performance, quality of egg, blood chemistry, and antioxidant ability in serum of laying chickens (Hisex Brown) reared from 22 to 34 weeks of age. Seventy-two 22-week-old laying hens (Hisex Brown) were haphazardly separated into 3 handling collections of 24 chickens (6 replicates per treatment and four laying hens per replicate). Dietary treatments included basal diet without zinc addition for control group while the 2nd and 3rd groups contained basal diet with 25 or 75 mg ZnO/kg diet. Results showed that the higher level of ZnO (75 mg ZnO/kg diet) elevated (P < 0.01) feed intake during all studied periods compared with the control group and other groups that contained ZnO. The handling groups supplied with 75 mg ZnO/kg diet gave the worst feed: egg ratio within the whole period and the intervals compared with the control and other ZnO levels. Supplementation of zinc decreased egg number and egg output when compared with the control groups. Egg quality traits were statistically differed due to dietary ZnO supplementation except egg shape index, yolk %, and albumin %. Supplementation of zinc decreased triglyceride (P = 0.001) of laying hens. The low-density lipoproteins (LDL) cholesterol level in serum was decreased with 75 mg ZnO/kg in comparison with all treatment groups. Zinc supplementation increased the level of serum zinc without differences in supplemented zinc levels. Dietary supplemental zinc did not affect antioxidant parameters in the serum. It is concluded that dietary zinc supplementation up to 75 mg/kg used as effective supplement to enhance zinc status and antioxidant ability and activities in laying hens.


Assuntos
Antioxidantes/metabolismo , Galinhas/fisiologia , Óvulo/fisiologia , Óxido de Zinco/metabolismo , Ração Animal/análise , Animais , Análise Química do Sangue/veterinária , Galinhas/sangue , Dieta/veterinária , Suplementos Nutricionais/análise , Relação Dose-Resposta a Droga , Feminino , Óvulo/efeitos dos fármacos , Óxido de Zinco/administração & dosagem
19.
Animal ; 14(1): 66-77, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31317849

RESUMO

Feed withdrawal (FW) is a frequent issue in open outdoor feedlot systems, where unexpected circumstances can limit the animals' access to food. The relationship among fasting period, animal behaviour during feed reintroduction (FR) and acidosis occurrence has not been completely elucidated. Twenty steers fitted with rumen catheters were fed a high-concentrate diet (concentrate : forage ratio 85 : 15) and were challenged by a protocol of FW followed by FR. The animals were randomly assigned to one of the four treatments: FW for 12 h (T12), 24 h (T24), 36 h (T36) or no FW (control group) followed by FR. The steers' behaviour, ruminal chemistry, structure of the ruminal microbial community, blood enzymes and metabolites and ruminal acidosis status were assessed. Animal behaviour was affected by the FW-FR challenge ( P < 0.05). Steers from the T12, T24 and T36 treatments showed a higher ingestion rate and a lower frequency of rumination. Although all animals were suspected to have sub-acute ruminal acidosis (SARA) prior to treatment, a severe case of transient SARA arose after FR in the T12, T24 and T36 groups. The ruminal pH remained below the threshold adopted for SARA diagnosis ( pH value = 5.6) for more than three consecutive hours (24, 7 and 19 h in the T12, T24 and T36 treatments, respectively). The FW-FR challenge did not induce clinical acute ruminal acidosis even though steers from the T36 treatment presented ruminal pH values that were consistent with this metabolic disorder (pH threshold for acute acidosis = 5.2). Total mixed ration reintroduction after the withdrawal period reactivated ruminal fermentation as reflected by changes in the fermentation end-products. Ruminal lactic acid accumulation in steers from the T24 and T36 treatments probably led to the reduction of pH in these groups. Both the FW and the FR phases may have altered the structure of the ruminal microbiota community. Whereas fibrolytic bacterial groups decreased relative abundance in the restricted animals, both lactic acid producer and utiliser bacterial groups increased ( P < 0.05). The results demonstrated a synchronisation between Streptococcus (lactate producer) and Megasphaera (lactate utiliser), as the relative abundance of both groups increased, suggesting that bacterial resilience may be central for preventing the onset of metabolic disturbances such as ruminal acidosis. A long-FW period (36 h) produced rumen pH reductions well below and lactic acid concentration increased well above the accepted thresholds for acute acidosis without any perceptible clinical signs.


Assuntos
Acidose/veterinária , Criação de Animais Domésticos/métodos , Doenças dos Bovinos/fisiopatologia , Privação de Alimentos/fisiologia , Acidose/etiologia , Acidose/fisiopatologia , Ração Animal , Animais , Análise Química do Sangue/veterinária , Bovinos/sangue , Doenças dos Bovinos/etiologia , Dieta/veterinária , Masculino , Distribuição Aleatória , Fatores de Tempo
20.
Trop Anim Health Prod ; 52(2): 829-837, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31520340

RESUMO

The effects of feeding corn steep liquor (CSL; 420 g/kg crude protein, DM basis) along with different cereal grains on performance, digestibility, blood metabolites, ruminal fermentation, and carcass characters of growing lambs were evaluated. The constant amount of CSL was included in basal diet (100 g/kg, DM basis) and grain sources as experimental treatments were as follows: (1) corn grain (CG), (2) barley grain (BG), or (3) wheat grain (WG). The eighteen individually fed Farahani lambs averaging body weight 32 kg were allocated in completely randomized design (6 lambs/each) in a 9-week trial. The results showed that the greatest intake and gain were found in lambs fed CG in contrast to others. Nitrogen intake was constant among diets; however, the greatest nitrogen efficiency was found for corn grain-fed animals. Digestibility of nutrients were reduced in WG-fed animals in comparison with other grains. Ruminal proportions of propionate and butyrate were reduce in WG-fed lambs. The CG-fed animals displayed greater blood glucose and lower BUN concentrations compared with others. The greatest aspartate aminotransferase concentration as well as the greatest liver fat deposition suggested a dysfunction in liver performance in WG-fed animals. Except than that of a tendency for increment in dressing percentage in CG-fed lambs, no carcass character was differed among treatments. In conclusion, results revealed that feeding liquid protein source (CSL) is recommendable when it has been fed along with corn grain in comparison with barley or wheat grains in growing lambs.


Assuntos
Proteínas na Dieta/metabolismo , Digestão , Carne/análise , Rúmen/metabolismo , Carneiro Doméstico/fisiologia , Ração Animal/análise , Fenômenos Fisiológicos da Nutrição Animal , Animais , Análise Química do Sangue/veterinária , Dieta/veterinária , Proteínas na Dieta/administração & dosagem , Grão Comestível/química , Fermentação , Carneiro Doméstico/crescimento & desenvolvimento
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...