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1.
Se Pu ; 37(10): 1105-1111, 2019 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642290

RESUMO

An analytical method by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed for the simultaneous determination of the two types of nutrients, viz. gingerol-related compounds and curcuminoids in ginger, including 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol, 10-shogaol, tetrahydrocurcumin, curcumin, demethoxycurcumin and bisdemethoxycurcumin. The 10 target compounds were separated on a ZORBAX RRHD Eclipse Plus C18 column (100 mm×2.1 mm, 1.8 µm) using gradient elution with 0.1% (v/v) formic acid aqueous solution and methanol containing 0.1% (v/v) formic acid as the mobile phases. Qualitative confirmation and quantitative analysis of the target compounds were performed using an electrospray ionization (ESI) source in the positive ion and multiple reaction monitoring (MRM) modes. The linear correlation coefficients (r) of the 10 nutrients were ≥ 0.9995. The limits of quantification were 0.10-7.71 µg/L. The average spiked recoveries of the samples at the three levels were 82.8%-115.3%, the relative standard deviations (RSDs) were 0.58%-11.49%. The results showed that all the 10 nutrients in ginger were detected, and the content of 6-gingerol was the highest at 373.35-702.48 mg/kg. This method is convenient, rapid, accurate and reliable, and is suitable for the analysis of gingerol-related and curcuminoids in ginger. It provides a technical means for the quality identification and control of ginger.


Assuntos
Análise de Alimentos/métodos , Gengibre/química , Nutrientes/análise , Compostos Fitoquímicos/análise , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem
2.
Se Pu ; 37(11): 1173-1178, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642269

RESUMO

A rapid screening method was developed to determine sibutramine and five derivatives in health food by high performance liquid chromatography-quadrupole/electrostatic orbitrap high-resolution mass spectrometry (HPLC-Q/Orbitrap HRMS). The sample was extracted with methanol via ultrasonic-assisted extraction coupled with high-speed centrifugation. Separation was performed on a Hypersil Gold column (100 mm×2.1 mm, 3 µm) by gradient elution with methanol/water (containing 0.15%(v/v) formic acid) as the mobile phase. The positive full mass scan/date-dependent MS2 (Full MS/dd-MS2) mode was used during MS detection, and quantification was achieved by resolution of the precursor mass. The analytes in the sample were separated, and accurate mass and MS2 fragment ions were simultaneously attained within 8 min. The results indicated that the obtained mass accuracy errors of the six analytes were less than 1×10-6. Good linearities were obtained in the range of 0.5-20.0 µg/L, and all correlation coefficients were higher than 0.999. The limits of quantification were 25 µg/kg and the recoveries were in the range of 93.5%-103.5% with relative standard deviations of 1.5%-7.7%. This simple-pretreatment, rapid, accurate, high-sensitivity, and high-selectivity method can be used in the rapid screening and quantitative analysis of sibutramine and its derivatives in health food.


Assuntos
Ciclobutanos/análise , Análise de Alimentos/métodos , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas , Eletricidade Estática
3.
Shokuhin Eiseigaku Zasshi ; 60(4): 108-112, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31474651

RESUMO

Assuming the intentional adulteration of beverages with plant toxins, an LC-MS/MS method for the simultaneous determination of 18 plant toxins (lycorine, galantamine, ricinine, scopolamine, gelsemine, atropine, colchicine, α-solanine, jervine, α-chaconine, veratramine, mesaconitine, digoxin, protoveratrine A, aconitine, hypaconitine, oleandrin, and digitoxin) was developed. As analytical samples, beer, distilled spirits, blend tea, ready-to-drink (RTD) coffee, and fermented milk drink were selected. The extraction and purification of the analytes were performed using modified QuEChERS method. Method validation in terms of intra-day precision, accuracy, and extraction recovery obtained satisfactory results. The calibration curves for the analytes were linear from 5 to 200 ng/mL (r>0.990), which enabled the determination of toxins in even trace amounts.


Assuntos
Bebidas/análise , Cromatografia Líquida , Análise de Alimentos/métodos , Espectrometria de Massas em Tandem , Toxinas Biológicas/análise
4.
J Agric Food Chem ; 67(39): 10968-10976, 2019 Oct 02.
Artigo em Inglês | MEDLINE | ID: mdl-31487165

RESUMO

Food contact materials (FCMs) may release their chemical components into food and thus raise safety concerns. This paper attempted to study the presence of four major groups of FCM-related endocrine disruptors in fatty food: dialkyl phthalates, bisphenols, printing ink photoinitiators, and polyfluoroalkyl substances. All 41 target compounds were analyzed simultaneously by means of liquid chromatography coupled to tandem mass spectrometry. The sample preparation was significantly streamlined to reduce analysis costs by employing acetonitrile extraction, extract modification by water, and refrigeration at 5 °C. The new method was validated and applied to 60 real samples, including edible oils, butter, and chocolate, where 16 target compounds were measured at levels ≤13000 ng/g. The study also described the blank level increase and sensitivity loss caused by impurities present in the HPLC methanol solvent.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Espectrometria de Massas em Tandem/métodos , Compostos Benzidrílicos/análise , Fenóis/análise , Ácidos Ftálicos/análise
5.
Shokuhin Eiseigaku Zasshi ; 60(3): 61-67, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31391412

RESUMO

A method was developed for the determination of nonvolatile amines, such as histamine, tyramine, putrescine, and cadaverine, in foods. These nonvolatile amines were extracted from a sample with 5% trichloroacetic acid, and the extract was purified using an InertSep MC-1 cartridge column. The four amines were derivatized with fluorescamine, determined by HPLC with a fluorescence detector, and confirmed by LC-MS/MS. The average recoveries (n=5) and the relative standard deviations from 11 foods (pacific saury, dried mackerel, canned mackerel in brine, canned tuna in oil, fish sauce, surimi, rice-koji miso, soy sauce, gouda cheese, red wine, and beer) spiked at 100 mg/kg were 81-100% and 0.4-3.1%, respectively.


Assuntos
Aminas/análise , Fluorescamina , Análise de Alimentos/métodos , Animais , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem
6.
Shokuhin Eiseigaku Zasshi ; 60(3): 68-72, 2019.
Artigo em Japonês | MEDLINE | ID: mdl-31391413

RESUMO

A rapid, sensitive and selective analytical method by LC-MS/MS was developed and validated for the determination of cyclamate in various kinds of foods. The Preparation of test solutions was performed by heat extraction technique in accordance with an official notification method in Japan. We aimed to reduce the matrix effects in LC-MS/MS only by diluting extracts without clean-up using solid phase column. This method was assessed for 30 kinds of foods fortifying cyclamate at the concentration level of 0.5 µg/g. As a result, trueness was 85.0 to 106.6%, repeatability (RSDr) ranged from 1.7 to 9.4%, and within-laboratory reproducibility (RSDwr) ranged from was 4.1 to 9.7%. These date supported the reliability our method. Thus, this method could be useful for a rapid determination of cyclamate in various kinds of foods.


Assuntos
Ciclamatos/análise , Análise de Alimentos/métodos , Cromatografia Líquida , Japão , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem
7.
Phys Chem Chem Phys ; 21(35): 19288-19297, 2019 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-31451821

RESUMO

This paper reports a facile, fast, and cost-effective method for the synthesis of three-dimensional (3D) porous AgNPs/Cu composites as SERS substrates for the super-sensitive and quantitative detection of food organic contaminations. Due to the 3D porous hotspot architecture and the strong plasmonic coupling between Ag and Cu, the porous AgNPs/Cu substrate achieves ultrasensitive detection of multiple analytes as low as 10-11 M (crystal violet, CV), 10-9 M (malachite green, MG), 10-11 M (acephate), and 10-9 M (thiram) even with a portable Raman device. Moreover, this 3D solid substrate has good signal uniformity (RSD < 11%) and superior stability (<14% signal loss), allowing for practical SERS detections. Importantly, by simply wiping the real sample surface using the substrate, it successfully detects CV and MG residues on crayfish, and the limit of detection (LOD) of CV and MG is determined to be 1.14 × 10-9 M and 0.94 × 10-7 M, respectively. Further, the substrate can also be applied to detect acephate on eggplant with a LOD of 1.41 × 10-9 M and thiram on an apple surface with a LOD of 1.04 × 10-7 M. Note that all these SERS detections on real samples have a broad dynamic concentration range and a good linear dependence. As a "proof of concept", multi-component detection on a real sample has also been demonstrated. This 3D solid substrate possesses excellent detection sensitivity, diversity, and accuracy, which allows rapid and reliable determination of toxic substances in foods.


Assuntos
Técnicas de Química Analítica/métodos , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Praguicidas/análise , Análise Espectral Raman , Animais , Técnicas de Química Analítica/economia , Cobre/química , Limite de Detecção , Nanopartículas Metálicas/química , Reprodutibilidade dos Testes , Prata/química
8.
J Agric Food Chem ; 67(37): 10543-10551, 2019 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-31464438

RESUMO

Adulteration of meat and meat products causes concerns to consumers. It is necessary to develop novel robust and sensitive methods that can authenticate the origin of meat by qualitative and quantitative means to minimize the drawbacks of the existing methods. This study has shown that the protein N-glycosylation profiles of different meats are species specific and thus can be used for meat authentication. Based on the N-glycan pattern, the investigated five meat species (beef, chicken, pork, duck, and mutton) can be distinguished by principal component analysis, and partial least square regression was performed to build a calibration and validation model for the prediction of adulteration ratio. Using this method, beef samples adulterated with a lower-value duck meat could be detected down to the addition ratio as low as 2.2%. The most distinguishing N-glycans from beef and duck were elucidated for the detailed structures.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Glicômica/métodos , Polissacarídeos/química , Animais , Bovinos , Galinhas , Análise Discriminante , Patos , Glicosilação , Carne , Análise de Componente Principal , Carne Vermelha , Suínos
9.
Food Chem ; 301: 125261, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31377618

RESUMO

Some Eastern Asian countries deem pufferfish - especially its muscle - a culinary delight. Herein, molecular mass fingerprinting of soups prepared by Takifugu flavidus, Takifugu obscurus and Takifugu rubripes was established via matrix-assisted laser desorption/ionization - mass spectrometry (MALDI MS). Soup samples were directly analyzed by MALDI MS to collect mass spectra within 0-700 Da in a quick way, followed by principal component analysis to distinguish the different soups and to find out the distinctive compounds among the soups. High performance liquid chromatography - tandem MS (HPLC-MS/MS) was applied to identify the compounds. Nineteen compounds were identified from the HPLC-MS/MS data by using METLIN database. Through literature mining, we found that these compounds are closely related to the flavor, nutrition, and safety of pufferfish soups. This method can also be used as a facile way to distinguish between different pufferfish fillets when morphological characters have been damaged or destroyed.


Assuntos
Análise de Alimentos/métodos , Inocuidade dos Alimentos , Metabolômica , Valor Nutritivo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Paladar , Tetraodontiformes/metabolismo , Animais , Culinária , Análise de Componente Principal
10.
J Sci Food Agric ; 99(14): 6628-6637, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31393605

RESUMO

BACKGROUND: Many studies have confirmed a wide variation in the phenolic content and antioxidant activity of beers. However, when commercial beers are studied, there is usually no information available on the brewing technology applied. In this study, technological parameters were varied systematically to influence the antioxidant content of beer with a view to improving its flavor stability. High-throughput assays, ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC) were investigated as fast analytical methods to evaluate the influence of brewing technology on antioxidant activity. RESULTS: Beers (n = 12) were brewed with systematic technological variations (malt modification, hopping regime) to influence the antioxidant potential. A late hop addition resulted in significantly higher phenolic content (high-performance liquid chromatography with diode-array detection - HPLC-DAD) and antioxidant activity. Raw protein content and malt modification significantly influenced phenolic content and the antioxidant activity of beers hopped at the beginning of wort boiling. Samples were stored under forced and natural conditions and were evaluated by a sensory panel. The decline of bitter iso-α-acids as an analytical marker for oxidative aging was significantly lower in beers brewed from malts with high raw protein content. These samples also had higher antioxidant activity values. Panelists gave higher ratings for beer quality to aged beers with a late hop addition. However, late hopping resulted in enhanced hoppy aroma attributes and therefore an altered aroma profile. CONCLUSIONS: Both antioxidant capacity methods were well suited as fast methods to evaluate brewing raw material and technological influence on antioxidant activity. The appropriate choice of barley malt and the malting regime could be promising tools to enhance the antioxidant activity of traditionally hopped beers. © 2019 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.


Assuntos
Antioxidantes/análise , Cerveja/análise , Análise de Alimentos/métodos , Paladar , Humanos , Humulus/química , Capacidade de Absorbância de Radicais de Oxigênio , Fenóis/análise
11.
J Chromatogr A ; 1604: 460467, 2019 Oct 25.
Artigo em Inglês | MEDLINE | ID: mdl-31451194

RESUMO

This paper describes the method validation for the simultaneous determination of seven cysteinylated aldehydes, i.e. 2-substituted 1,3-thiazolidines-4-carboxylic acids, using ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS). Authentic reference compounds were first synthesized for identification and quantification purposes. Moreover, nuclear magnetic resonance (1H NMR and 13C NMR) was applied for verification of their structure, while ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) was applied for estimation of the purity. The method for quantification of cysteinylated aldehydes in model solutions has been validated according to the criteria and procedures described in international standards. The synthesized compounds were successfully identified via UHPLC-MS by comparing retention time and MS spectra with the commercial reference compounds. Method validation revealed good linearity (R2 > 0.995) over the range of 0.4-2.2 µg/L to approximately 1000 µg/L, depending on the analyte. The limits of quantification varied from 0.9 to 4.3 µg/L depending on the nature of the compound. Furthermore, evaluation of the method showed good accuracy and stability of the standard solutions. Reported chromatographic recoveries ranged from 112 to 120%. Consequently, the currently described method was applied on malt and beer samples. For the first time, quantification of cysteinylated aldehydes was obtained in malt. In contrast, in fresh beers unambiguous identification of these compounds was not achieved.


Assuntos
Aldeídos/análise , Cerveja/análise , Cromatografia Líquida de Alta Pressão , Análise de Alimentos/métodos , Espectrometria de Massas em Tandem
12.
Food Chem ; 298: 125088, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31260987

RESUMO

Infant formula certified reference material (CRM, KRISS CRM 108-02-003) were developed for the analysis of organic nutrients. The CRM is a milk-based infant formula powder, packaged at 14 g per unit. Ten thousand units were prepared and stored at -70 °C. For the certification of each nutrient, ten units were analyzed for simultaneous value-assignment and homogeneity test. Analytical methods used were isotope dilution mass spectrometry (IDMS) based on liquid chromatography mass spectrometer (LC/MS) or gas chromatography mass spectrometer (GC/MS) as higher-order reference methods.13 vitamins, 3 fatty acids, and total cholesterol were certified. The between-unit relative standard deviation of measurement results for each nutrient ranged 0.2% to 2.5%, showing very good homogeneity. The expanded relative uncertainties of the certified values ranged from 1% to 8%, indicating that they have higher-order metrological quality. The values of proximates (proteins, lipids, carbohydrates, water, and ash) were assigned through inter-laboratory comparisons.


Assuntos
Análise de Alimentos/métodos , Fórmulas Infantis/análise , Fórmulas Infantis/normas , Certificação , Colesterol/análise , Cromatografia Líquida/métodos , Ácidos Graxos/análise , Análise de Alimentos/normas , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Lactente , Nutrientes/análise , Padrões de Referência , Vitaminas/análise
13.
Food Chem ; 298: 125096, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31272051

RESUMO

The aim of this paper is to test different models for predicting furan content in a dough system, based on partial least squares regression using colour images. Starch dough systems were fried at five temperatures between 150 and 190 °C and for 5, 7, 9, 11 and 13 min. The furan content was quantified using gas chromatography/mass spectrometry, while the corresponding images were simultaneously obtained and processed in order to extract 2914 features. Good furan content predictions were obtained using computer vision image chromatic features using correlation coefficient of prediction (Rp = 0.86). However, the best prediction correlation was obtained using the image textural features (Rp = 0.93), when the number of features was reduced to 10 by algorithms applications. These results suggest that furan content in fried dough systems can be predicted using features of computer vision images.


Assuntos
Pão , Análise de Alimentos/métodos , Indústria de Processamento de Alimentos/métodos , Furanos/análise , Processamento de Imagem Assistida por Computador/métodos , Algoritmos , Cor , Culinária , Qualidade dos Alimentos , Cromatografia Gasosa-Espectrometria de Massas , Análise dos Mínimos Quadrados , Amido , Triticum
14.
Food Chem ; 298: 125100, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31272054

RESUMO

This study presents an HPLC-ESI-Q-TOF method for simultaneous quantification of short-chain chlorinated paraffins (SCCPs, C10-13) and an additional characterization of medium-chain chlorinated paraffins (MCCPs, C14-17) in oven-baked pastry products (n = 38) and unprocessed pastry dough material (n = 15). Almost 2 times higher SCCP levels were found in the dough material. ΣSCCP concentrations in products ranged from 0.3 ng g-1 to 23.0 ng g-1 (mean: 6.3 ng g-1), while the results for dough ranged from 5.8 ng g-1 to 22.8 ng g-1 (mean: 12.9 ng g-1). Regardless of the sample matrix, the most abundant CP homologue groups were hepta- and octa-chlorinated undecanes and dodecanes. The average chlorination degree found in dough samples fell within a range of 55-60% (w/w). Meanwhile, a slight decrease of CP chlorination degree was observed for oven-baked products, in particular for C10-C12 SCCPs, thus indicating that thermal decomposition of CPs occurs even under relatively mild conditions.


Assuntos
Pão/análise , Indústria de Processamento de Alimentos/métodos , Hidrocarbonetos Clorados/análise , Hidrocarbonetos Clorados/química , Parafina/química , Cromatografia Líquida de Alta Pressão , Farinha , Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Indústria de Processamento de Alimentos/instrumentação , Halogenação , Parafina/análise , Espectrometria de Massas por Ionização por Electrospray/métodos
15.
Food Chem ; 298: 125090, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31272055

RESUMO

In this study, molecular properties of wheat starch from three different types of breads were analyzed using asymmetric flow field-flow (AF4) connected to multi-angle light scattering (MALS) and differential refractive index (dRI) detectors. This analysis allowed the determination of molecular properties, i.e. molar mass (M), root-mean-square radius (rrms), apparent density (ρapp) and conformation. Complementary analyses, such as resistant starch and amylose content, were also performed. The results show that wheat starch extracted from breads can have different properties reflected in changes in M, rrms and ρapp. In addition, the results suggest that some of the changes in molecular properties may be related to the presence of resistant starch.


Assuntos
Pão/análise , Fracionamento por Campo e Fluxo/métodos , Refratometria/métodos , Amido/química , Triticum/química , Amilose/análise , Bolívia , Análise de Alimentos/métodos , Peso Molecular , Espalhamento de Radiação
16.
Food Chem ; 299: 125107, 2019 Nov 30.
Artigo em Inglês | MEDLINE | ID: mdl-31302428

RESUMO

Traceability and authenticity is crucial to the food safety of scallop. The present study investigated the possibility of using stable isotope analysis to identify the origins and species of scallops (Patinopecten yessoensis, Chlamys farreri, and Argopecten irradians) in the coastal areas of China. The δ13C and δ15N values of a total of 575 samples from seven sites around China were determined and additional 150 samples were tested by fisher linear discrimination analysis (LDA) to estimate the accuracy of origin identification and species prediction. The results show that the stable C and N isotope composition differed significantly depending on the origin, season and species of scallops. Meanwhile, the LDA shows that 92% of the samples were correctly classified for origin prediction, and an accuracy of 98.3% was obtained for species prediction. This study reveals that stable isotope ratio is an effective technique to trace the geographical origin and identify the species of scallops.


Assuntos
Isótopos de Carbono/análise , Análise de Alimentos/métodos , Isótopos de Nitrogênio/análise , Pectinidae/química , Frutos do Mar/análise , Animais , China , Análise Discriminante , Análise de Alimentos/estatística & dados numéricos , Especificidade da Espécie
17.
J Chromatogr A ; 1602: 178-187, 2019 Sep 27.
Artigo em Inglês | MEDLINE | ID: mdl-31301797

RESUMO

A magnetic porous covalent triazine-based organic polymer (M-PCTP) was synthesized by co-precipitation method. The M-PCTP combined both the properties of the PCTP and the magnetism of Fe3O4 nanoparticles, possessing highly porous structure and good magnetism. It was used as a magnetic solid phase extraction (MSPE) adsorbent to extract carbamate pesticides (propoxur, carbaryl, isoprocarb, fenobcarb and diethofencarb) from lemonade and grape juice samples prior to high performance liquid chromatography-mass spectrometric detection. Under the optimal conditions, a good linearity for the real samples was received in the range from 0.10-0.90 to 80.00 ng mL-1 with the correlation coefficients of 0.9882-0.9983. The method recoveries for the five carbamates were 86.3-108.0%. The limits of detection were 0.02-0.20 ng mL-1 for lemonade sample, and 0.04-0.30 ng mL-1 for grape juice sample. The M-PCTP also demonstrated good extraction capabilities for other different kinds of organic compounds including chlorophenols and polycyclic aromatic hydrocarbons.


Assuntos
Carbamatos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Análise de Alimentos/métodos , Espectrometria de Massas , Praguicidas/análise , Extração em Fase Sólida , Sucos de Frutas e Vegetais/análise , Magnetismo , Hidrocarbonetos Policíclicos Aromáticos/análise , Polímeros/química , Porosidade
18.
J Agric Food Chem ; 67(31): 8425-8430, 2019 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-31322874

RESUMO

In recent years, non-targeted methods have been a popular "buzz" phrase in food fraud detection. Using analytical instrumentation techniques, non-targeted methods have been developed and applied in many food and agricultural situations. However, confusion and misstatements remain regarding how the methods are used. This perspective will discuss the definitions related to non-targeted testing, the procedure of developing and validating methods, the techniques and data analysis, and opportunities and challenges regarding the use of this class of analytical methods. The perspective seeks to provide readers with the latest information regarding recent advances in the use of non-targeted methods.


Assuntos
Análise de Alimentos/métodos , Contaminação de Alimentos/análise , Cromatografia Líquida/métodos , Eletroforese Capilar/métodos , Análise de Alimentos/instrumentação , Humanos , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos
19.
Food Chem ; 300: 125173, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31319335

RESUMO

The administration of anabolic agents in farm animals to improve meat production has been prohibited in EU, due to the potential risks to human health. Meat quality was investigated to detect the effects of illegal administration of dexamethasone or prednisolone or 17ß-estradiol on Charolais bulls. Three groups of 6 bulls were treated and 12 bulls were the control. Meat quality parameters were measured on live animals, carcasses and on samples of Longissimus thoracis and multivariate statistical data analysis was applied. In Charolais bulls, these parameters were affected by growth promoter administration and the multivariate canonical discriminant analysis was able to distinguish between treated and untreated animals mainly due to three electronic nose's parameters, 24 h carcass temperature and drip loss. Therefore, meat quality control and the multivariate analysis could be useful as a first screening to address targeted controls on farms suspected of illicit use of growth promoters.


Assuntos
Análise de Alimentos/métodos , Substâncias de Crescimento/farmacologia , Carne , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/crescimento & desenvolvimento , Animais , Bovinos , Dexametasona/farmacologia , Análise Discriminante , Nariz Eletrônico , Estradiol/farmacologia , Fazendas , Análise de Alimentos/instrumentação , Análise de Alimentos/estatística & dados numéricos , Qualidade dos Alimentos , Masculino , Carne/análise , Prednisolona/farmacologia
20.
Food Chem ; 300: 125175, 2019 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-31323606

RESUMO

Despite the ban of nitrofurans (NFs) for use in food production in many countries in the 1990s, NF metabolites in food are still regularly detected during import control testing. We have developed a confirmatory routine method for the detection and quantification of NF metabolites in seafood using LC-MS/MS and validated the method according to the strict criteria in European legislation and Codex Alimentarius. Method characteristics were found to fulfill the criteria. We report for the first time a new false positive for 1-amino-2,4-imidazolidinedione (AHD), the metabolite of Nitrofurantoin (NFT). By using optimized washing procedures, the non tissue bound false positives can be minimized. The results from the validation on both lean and fatty fish and crustaceans, results from proficiency tests and routine use over many years, demonstrates that the method is fit for purpose to determine NF metabolites in the seafood category.


Assuntos
Cromatografia Líquida/métodos , Contaminação de Alimentos/análise , Nitrofurantoína/análise , Alimentos Marinhos/análise , Espectrometria de Massas em Tandem/métodos , Animais , Reações Falso-Positivas , Análise de Alimentos/métodos , Técnicas de Diluição do Indicador , Nitrofurantoína/metabolismo
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