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1.
J Vet Sci ; 21(4): e65, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32735101

RESUMO

BACKGROUND: Aleutian mink disease virus (AMDV) causes major economic losses in fur-bearing animal production. The control of most AMDV outbreaks is complex due to the difficulties of establishing the source of infection based only on the available on-farm epidemiological data. In this sense, phylogenetic analysis of the strains present in a farm may help elucidate the origin of the infection and improve the control and biosecurity measures. OBJECTIVES: This study had the following aims: characterize the AMDV strains from most outbreaks produced at Spanish farms between 2012-2019 at the molecular level, and assess the utility of the combined use of molecular and epidemiological data to track the possible routes of infection. METHODS: Thirty-seven strains from 17 farms were partially sequenced for the NS1 and VP2 genes and analyzed phylogenetically with other strains described worldwide. RESULTS: Spanish AMDV strains are clustered in four major clades that generally show a good geographical correlation, confirming that most had been established in Spain a long time ago. The combined study of phylogenetic results and epidemiological information of each farm suggests that most of the AMDV outbreaks since 2012 had been produced by within-farm reservoirs, while a few of them may have been due to the introduction of the virus through international trade. CONCLUSIONS: The combination of phylogenetic inference, together with epidemiological data, helps assess the possible origin of AMDV infections in mink farms and improving the control and prevention of this disease.


Assuntos
Vírus da Doença Aleutiana do Vison/genética , Doença Aleutiana do Vison/epidemiologia , Surtos de Doenças/veterinária , Vison , Doença Aleutiana do Vison/virologia , Animais , França/epidemiologia , Variação Genética , Epidemiologia Molecular , Filogenia , Portugal/epidemiologia , Prevalência , Estudos Retrospectivos , Análise de Sequência de DNA/veterinária , Espanha/epidemiologia
2.
Vet Clin North Am Equine Pract ; 36(2): 195-209, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32654781

RESUMO

The sequencing and assembly of a reference genome for the horse has been revolutionary for investigation of horse health and performance. Next-generation sequencing (NGS) methods represent a second revolution in equine genomics. Researchers can align and compare DNA and RNA sequencing data to the reference genome to explore variation that may contribute or be attributed to disease. NGS has also facilitated the translation of research discovery to clinically relevant applications. This article discusses the history and development of NGS, details some of the available sequencing platforms, and describes currently available applications in the context of both discovery and clinical settings.


Assuntos
Sequenciamento de Nucleotídeos em Larga Escala/veterinária , Cavalos/genética , Análise de Sequência de DNA/veterinária , Animais , Genômica/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Doenças dos Cavalos/genética , Análise de Sequência de DNA/métodos
3.
Arch Razi Inst ; 75(2): 155-162, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32621443

RESUMO

Coronaviruses (AvCoV) which include infectious bronchitis virus (IBV) and other bird coronaviruses belong to the genus gammacoronavirus, subfamily Coronavirinae. One of the most prominent representatives of gammacoronavirus genus is infectious bronchitis virus (IBV) which is a highly contagious viral pathogen of chickens causing considerable economic losses to the poultry industry. IBVs mostly affect the respiratory, urinary, and reproductive tracts leading to a substantial drop in production. Backyard poultry in the villages usually share their food and water with free flight birds which puts them at serious risk of disease transmission. Furthermore, the poor hygienic measurements which are often used in backyard flocks make them a potential reservoir for diseases that can be transferred to commercial poultry flocks. Live bird markets (LBMs) which receive live poultry to be resold or slaughtered and sold onsite play a significant role in spreading infectious diseases among the different bird species. In the present study, a number of 354 cloacal swab samples were collected from different bird species from LBMs of Gilan province. Subsequently, after RNA extraction, reverse transcription-polymerase chain reaction (RT-PCR) technique was carried out using specific primers of S1 gene to detect coronavirus infectious bronchitis virus. Two samples from backyard chickens were reported to be positive to coronavirus which were named Iran/Backyardchicken 96/2017 and Iran/Backyardchicken 94/2017. The results of the phylogenetic analysis demonstrated that these two isolates are placed in QX and IS-1494 strains, respectively. On a final note, the obtained results highlighted the role of live birds offered in LBMs in the epidemiology of IBV and the transmission of the virus to the industrial flock.


Assuntos
Galinhas/virologia , Infecções por Coronavirus/veterinária , Vírus da Bronquite Infecciosa , Doenças das Aves Domésticas/virologia , Animais , Cloaca/virologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/virologia , Vírus da Bronquite Infecciosa/genética , Irã (Geográfico)/epidemiologia , Filogenia , Doenças das Aves Domésticas/diagnóstico , Doenças das Aves Domésticas/epidemiologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Análise de Sequência de DNA/veterinária
4.
J Vet Diagn Invest ; 32(4): 611-615, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32687008

RESUMO

The only Sarcocystis species currently known to inhabit the fibers of skeletal and cardiac muscles in horses are S. fayeri, S. bertrami, and S. asinus. We describe herein the invasion of myofibers in a horse by S. gigantea, a sheep-specific species with low virulence in the original host. A hunter gelding was referred to a veterinary surgeon in Newmarket (UK). The anamnestic data reported that the horse had an initial history of swelling of the right forelimb with fluid on the front of the carpus and edema spreading up the forearm. Subsequently, 2 firm lumps were found on the left pectoral muscle adjacent to the axilla of the left forelimb. Histologic examination of biopsies from the lumps revealed multifocal granulomatous eosinophilic myositis associated with intact and degenerate encysted parasites, consistent with Sarcocystis spp. Based on amplification and DNA sequencing of the 18S rRNA gene obtained from formalin-fixed, paraffin-embedded tissue blocks, S. gigantea was identified. The presence of sarcocysts in equine skeletal muscles has been considered an incidental finding, and there are only sporadic associated reports of myositis. Our finding suggests that some Sarcocystis spp. have a wider intermediate host range than believed previously, and that Sarcocystis of other species (not considered horse-associated) can invade the muscle fibers of equids, leading to myositis.


Assuntos
Doenças dos Cavalos/patologia , Miosite/veterinária , Sarcocystis/isolamento & purificação , Sarcocistose/veterinária , Animais , Cavalos , Masculino , Distrofia Muscular do Cíngulo dos Membros/diagnóstico , Distrofia Muscular do Cíngulo dos Membros/parasitologia , Distrofia Muscular do Cíngulo dos Membros/patologia , Miosite/diagnóstico , Miosite/parasitologia , Miosite/patologia , RNA de Protozoário/análise , RNA Ribossômico 18S/análise , Sarcocistose/patologia , Análise de Sequência de DNA/veterinária
5.
J Vet Sci ; 21(3): e40, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32476314

RESUMO

The purpose of this study was to investigate the high-level mupirocin resistance (HLMR) in Gram-positive bacteria isolated from companion animals. A total of 931 clinical specimens were collected from diseased pets. The detection of mupirocin-resistant bacteria and plasmid-mediated mupirocin resistance genes were evaluated by antimicrobial susceptibility tests, polymerase chain reactions, and sequencing analysis. Four-hundred and six (43.6%) bacteria were isolated and 17 (4.2%), including 14 staphylococci and 3 Corynebacterium were high-level mupirocin-resistant (MICs, ≥ 1,024 ug/mL) harboring mupA. Six staphylococci of HLMR strains had plasmid-mediated mupA-IS257 flanking regions. The results show that HLMR bacteria could spread in veterinary medicine in the near future.


Assuntos
Antibacterianos/farmacologia , Corynebacterium/efeitos dos fármacos , Farmacorresistência Bacteriana , Mupirocina/farmacologia , Staphylococcus/efeitos dos fármacos , Animais , Doenças do Gato/tratamento farmacológico , Gatos , Infecções por Corynebacterium/tratamento farmacológico , Doenças do Cão/tratamento farmacológico , Cães , Testes de Sensibilidade Microbiana/veterinária , Reação em Cadeia da Polimerase/veterinária , Análise de Sequência de DNA/veterinária , Infecções Estafilocócicas/tratamento farmacológico
6.
PLoS One ; 15(5): e0232542, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32379844

RESUMO

Umbilical hernia (UH) is one of the most frequent defects affecting pig production, however, it also affects humans and other mammals. UH is characterized as an abnormal protrusion of the abdominal contents to the umbilical region, causing pain, discomfort and reduced performance in pigs. Some genomic regions associated to UH have already been identified, however, no study involving RNA sequencing was performed when umbilical tissue is considered. Therefore, here, we have sequenced the umbilical ring transcriptome of five normal and five UH-affected pigs to uncover genes and pathways involved with UH development. A total of 13,216 transcripts were expressed in the umbilical ring tissue. From those, 230 genes were differentially expressed (DE) between normal and UH-affected pigs (FDR <0.05), being 145 downregulated and 85 upregulated in the affected compared to the normal pigs. A total of 68 significant biological processes were identified and the most relevant were extracellular matrix, immune system, anatomical development, cell adhesion, membrane components, receptor activation, calcium binding and immune synapse. The results pointed out ACAN, MMPs, COLs, EPYC, VIT, CCBE1 and LGALS3 as strong candidates to trigger umbilical hernias in pigs since they act in the extracellular matrix remodeling and in the production, integrity and resistance of the collagen. We have generated the first transcriptome of the pig umbilical ring tissue, which allowed the identification of genes that had not yet been related to umbilical hernias in pigs. Nevertheless, further studies are needed to identify the causal mutations, SNPs and CNVs in these genes to improve our understanding of the mechanisms of gene regulation.


Assuntos
Hérnia Umbilical/veterinária , Doenças dos Suínos/genética , Animais , Perfilação da Expressão Gênica/veterinária , Predisposição Genética para Doença/genética , Hérnia Umbilical/genética , Reação em Cadeia da Polimerase , Locos de Características Quantitativas/genética , Análise de Sequência de DNA/veterinária , Suínos/genética
7.
PLoS Genet ; 16(5): e1008742, 2020 05.
Artigo em Inglês | MEDLINE | ID: mdl-32392208

RESUMO

The rhesus macaque is an abundant species of Old World monkeys and a valuable model organism for biomedical research due to its close phylogenetic relationship to humans. Copy number variation is one of the main sources of genomic diversity within and between species and a widely recognized cause of inter-individual differences in disease risk. However, copy number differences among rhesus macaques and between the human and macaque genomes, as well as the relevance of this diversity to research involving this nonhuman primate, remain understudied. Here we present a high-resolution map of sequence copy number for the rhesus macaque genome constructed from a dataset of 198 individuals. Our results show that about one-eighth of the rhesus macaque reference genome is composed of recently duplicated regions, either copy number variable regions or fixed duplications. Comparison with human genomic copy number maps based on previously published data shows that, despite overall similarities in the genome-wide distribution of these regions, there are specific differences at the chromosome level. Some of these create differences in the copy number profile between human disease genes and their rhesus macaque orthologs. Our results highlight the importance of addressing the number of copies of target genes in the design of experiments and cautions against human-centered assumptions in research conducted with model organisms. Overall, we present a genome-wide copy number map from a large sample of rhesus macaque individuals representing an important novel contribution concerning the evolution of copy number in primate genomes.


Assuntos
Mapeamento Cromossômico , Variações do Número de Cópias de DNA/fisiologia , Duplicação Gênica/fisiologia , Macaca mulatta/genética , Animais , Mapeamento Cromossômico/veterinária , Feminino , Genética Populacional , Genoma , Sequenciamento de Nucleotídeos em Larga Escala/veterinária , Humanos , Macaca mulatta/classificação , Masculino , Fases de Leitura Aberta/genética , Filogenia , Análise de Sequência de DNA/veterinária , Especificidade da Espécie
8.
Avian Dis ; 64(1): 2-6, 2020 03.
Artigo em Inglês | MEDLINE | ID: mdl-32267119

RESUMO

We first report avipoxvirus (APV) infection and an isolate named APV/03/2016 from a red-flanked blue robin (Tarsiger cyanurus) captured at Songhua Lake Scenic Area in Jilin City (Jilin Province, China) on March 24, 2016. The partial sequence of the 4b core protein gene and DNA polymerase gene of APV/03/2016 suggests that the virus belongs to the subclade B1 cluster of clade B (canarypox virus). The BLAST results showed the highest similarity of the two genes with the Pacific shearwater-isolated strain SWPV-2 (KX857215), canarypox virus strain D98-11133 (GQ487567), canarypox virus strain ATCC VR-111 (AY318871), avipoxvirus Mississippi isolate P89 (KC018048), and avipoxvirus Wisconsin isolate P92 (KC018051). The results indicate that APV/03/2016 is a canarypox-like virus. These findings demonstrate the continuous emergence of new APV hosts such as red-flanked blue robins and suggest that monitoring of APV circulation and evolution should be strengthened for T. cyanurus conservation.


Assuntos
Avipoxvirus/isolamento & purificação , Doenças das Aves/virologia , Infecções por Poxviridae/veterinária , Aves Canoras , Animais , Avipoxvirus/classificação , Avipoxvirus/genética , China , DNA Viral/análise , Filogenia , Infecções por Poxviridae/virologia , Análise de Sequência de DNA/veterinária
9.
Arch Virol ; 165(6): 1409-1417, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32318833

RESUMO

Chicken anemia virus (CAV) causes severe anemia and immunosuppression in young chickens and a compromised immune response in older birds, resulting in great economic losses to the poultry industry worldwide. Here, we report the molecular epidemiology and characterization of CAV circulating in poultry in Guangdong province, China. Ninety-one of 277 chickens collected from 2016 to 2017 were CAV positive. Full-genome sequencing revealed the presence of eight separate strains. Phylogenetic analysis based on the genome sequences obtained in this study and related sequences available in the GenBank database showed that all of the CAV isolates exhibit a close relationship to each other and belong to the same genotypic group. Putative recombination events were also detected in the genomes of the newly isolated CAVs. Collectively, our findings underscore the importance of CAV surveillance and provide information that will lead to a better understanding of the evolution of CAV.


Assuntos
Vírus da Anemia da Galinha/classificação , Infecções por Circoviridae/veterinária , Variação Genética , Genótipo , Doenças das Aves Domésticas/virologia , Recombinação Genética , Animais , Sequência de Bases , Vírus da Anemia da Galinha/isolamento & purificação , Galinhas , China/epidemiologia , Infecções por Circoviridae/virologia , Epidemiologia Molecular , Filogenia , Análise de Sequência de DNA/veterinária
10.
J Vet Sci ; 21(2): e31, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32233137

RESUMO

In this study, whiteleg shrimp (Penaeus vannamei) imported from Vietnam were collected from South Korean markets, and examined for 2 viruses: infectious hypodermal and hematopoietic necrosis virus (IHHNV, recently classified as decapod penstyldensovirus-1), and white spot syndrome virus (WSSV). Among 58 samples, we detected IHHNV in 23 samples and WSSV in 2 samples, using polymerase chain reaction and sequencing analyses. This is the first report of IHHNV and WSSV detection in imported shrimp, suggesting that greater awareness and stricter quarantine policies regarding viruses infecting shrimp imported to South Korea are required.


Assuntos
Densovirinae/isolamento & purificação , Microbiologia de Alimentos , Penaeidae/virologia , Alimentos Marinhos/virologia , Vírus da Síndrome da Mancha Branca 1/isolamento & purificação , Animais , República da Coreia , Análise de Sequência de DNA/veterinária , Vietnã
11.
J Dairy Sci ; 103(6): 5278-5290, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32331872

RESUMO

The cattle reference genome assembly has underpinned major innovations in beef and dairy genetics through genome-enabled selection, including removal of deleterious recessive variants and selection for favorable alleles affecting quantitative production traits. The initial reference assemblies, up to and including UMD3.1 and Btau4.1, were based on a combination of clone-by-clone sequencing of bacterial artificial chromosome clones generated from blood DNA of a Hereford bull and whole-genome shotgun sequencing of blood DNA from his inbred daughter/granddaughter named L1 Dominette 01449 (Dominette). The approach introduced assembly gaps, misassemblies, and errors, and it limited the ability to assemble regions that undergo rearrangement in blood cells, such as immune gene clusters. Nonetheless, the reference supported the creation of genotyping tools and provided a basis for many studies of gene expression. Recently, long-read sequencing technologies have emerged that facilitated a re-assembly of the reference genome, using lung tissue from Dominette to resolve many of the problems and providing a bridge to place historical studies in common context. The new reference, ARS-UCD1.2, successfully assembled germline immune gene clusters and improved overall continuity (i.e., reduction of gaps and inversions) by over 250-fold. This reference properly places nearly all of the legacy genetic markers used for over a decade in the industry. In this review, we discuss the improvements made to the cattle reference; remaining issues present in the assembly; tools developed to support genome-based studies in beef and dairy cattle; and the emergence of newer genome assembly methods that are producing even higher-quality assemblies for other breeds of cattle at a fraction of the cost. The new frontier for cattle genomics research will likely include a transition from the individual Hereford reference genome, to a "pan-genome" reference, representing all the DNA segments existing in commonly used cattle breeds, bringing the cattle reference into line with the current direction of human genome research.


Assuntos
Bovinos/genética , Genoma , Genômica/instrumentação , Seleção Genética , Análise de Sequência de DNA/veterinária , Animais
12.
Vet Parasitol ; 280: 109091, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32208305

RESUMO

Neospora caninum is considered one of the main causes of abortion in cattle but can also cause abortion in sheep. There is limited knowledge of the N. caninum population infecting sheep, and only one N. caninum isolate from a pregnant sheep from Japan has been reported. This study describes the in vitro isolation and genetic characterization of two new sheep isolates of N. caninum implicated in ovine reproductive failure. We used IFN-γ-knockout mice inoculated with PCR-positive brain homogenates from two clinically healthy but congenitally infected lambs at 4.5 months of age for parasite isolation. The lambs were born to dams from a sheep farm that had experienced pregnancy failure caused by N. caninum in successive generations. Tachyzoites were microscopically visualized in peritoneal flushes from all inoculated mice and were also observed in MARC-145 cell cultures within one week after inoculation with peritoneal flushes. Two N. caninum isolates, Nc-Spain11 and Nc-Spain12, were obtained from each lamb. The genotyping of the Nc-Spain11 and Nc-Spain12 isolates based on 9 microsatellite markers showed identical multilocus genotype (MLG). Comparison between a previous N. caninum genotype dataset including 80 MLGs from Argentinean, Spanish, Mexican, German and Scottish bovine isolates and the Japanese sheep isolate showed that the Nc-Spain11 and Nc-Spain12 MLG was unique and differed from the other MLGs. eBURST analyses showed that the Nc-Spain11 and Nc-Spain12 MLG was genetically clustered with other bovine MLGs and one ovine MLG, and the nearest genetic relationship was with an MLG from a bovine abortion collected in the same geographical area of Galicia.


Assuntos
Coccidiose/veterinária , Neospora/isolamento & purificação , Doenças dos Ovinos/parasitologia , Animais , Coccidiose/parasitologia , Feminino , Interferons/deficiência , Masculino , Camundongos , Camundongos Knockout/parasitologia , Neospora/genética , Análise de Sequência de DNA/veterinária , Ovinos , Espanha
13.
Res Vet Sci ; 130: 110-117, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32171999

RESUMO

Cathepsin B is one member of cysteine protease family and widely distributed in organisms, it plays an important function in parasite penetrating, migrating, molting and immune escaping. The aim of this work was to investigate whether exist interaction between a Trichinella spiralis cathepsin B (TsCB) and mouse intestinal epithelium cells (IECs), and its influence in the process of larva cell invasion. The results of ELISA, indirect immunofluorescence assay (IIFA), confocal microscopy and Far western blotting showed that there was a strong specific binding of rTsCB and IEC proteins, and the binding positions were located in cytoplasm and nuclei of IECs. The results of the in vitro larva penetration test revealed that rTsCB facilitated the larva invasion of IECs, whereas anti-rTsCB antibodies impeded partially the larva intrusion of enterocytes, this promotive or inhibitory roles were dose-dependent of rTsCB or anti-rTsCB antibodies. Silencing TsCB by siRNA mediated RNA interference reduced the TsCB expression in T. spiralis larvae, and markedly inhibited the larva penetration of enterocytes. The results indicated that TsCB binding to IECs promoted larva penetration of host's enteral epithelia, and it is a promising molecular target against intestinal invasive stages of T. spiralis.


Assuntos
Catepsina B/genética , Enterócitos/parasitologia , Células Epiteliais/parasitologia , Proteínas de Helminto/genética , Mucosa Intestinal/parasitologia , Trichinella spiralis/fisiologia , Animais , Catepsina B/metabolismo , Feminino , Proteínas de Helminto/metabolismo , Larva/genética , Larva/crescimento & desenvolvimento , Larva/fisiologia , Camundongos , Camundongos Endogâmicos BALB C , Análise de Sequência de DNA/veterinária , Trichinella spiralis/genética , Trichinella spiralis/crescimento & desenvolvimento , Triquinelose/parasitologia
14.
Parasitol Int ; 76: 102103, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32169658

RESUMO

The redescription of Opalina obtrigonoidea Metcalf, 1923, collected from the rectum of the toads Duttaphrynus melanostictus, is presented in this paper based on detailed morphological information and molecular data. Our results revealed that O. obtrigonoidea varies greatly in body dimensions. Its morphological characteristics allow its differentiation from Opalina undulata. Surprisingly, we sequenced its SSU rDNA-ITS1-5.8S rDNA-ITS2-LSU rDNA (5' end) and found the SSU rDNA of O. obtrigonoidea is nearly identical to that of O. undulata. However, there are differences in both the ITS1 and ITS2 regions that allow their distinction and confirm the morphological differences. Our results indicate that O. obtrigonoidea and O. undulata are closely related species in which morphological and genetic markers have evolved at different speeds. Due to this, the SSU rDNA gene may not be a valid marker for inter-species identification in Opalina, but the ITS is a valid marker for differentiating species in this genus.


Assuntos
Bufonidae/parasitologia , Estramenópilas/classificação , Animais , China , DNA Espaçador Ribossômico/análise , Marcadores Genéticos , Microscopia Eletrônica de Varredura/veterinária , Filogenia , Análise de Sequência de DNA/veterinária , Estramenópilas/citologia , Estramenópilas/ultraestrutura
15.
Anim Genet ; 51(3): 423-429, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32162363

RESUMO

An embryonic lethal mutation in chicken named cleft primary palate (cpp) is inherited in an autosomal recessive mode and results in a severely truncated upper beak. In this study, genotyping and sequencing techniques were employed to advance our genetic and genomic knowledge of the mutation's chromosomal location, candidate region and possible causative element using a congenic inbred line. Herein, the candidate region for the cpp developmental mutation was established as a ca. 5.1 Mb region of chicken chromosome 11 (GGA 11) through the use of a 600K Affymetrix SNP array. The SNPs identified from this array linked to cpp were used to genotype individuals from the congenic inbred line over several generations and thereby fine-map the causative region resulting in an approximately 200 kb size reduction. This candidate region (4.9 Mb) was sequenced via capture array in a cohort of 24 individuals, including carriers, mutants and their wild type (wt) siblings. Interestingly, the GGA 11 region for cpp encompasses the predicted centromere location and is thus unlikely to be highly disrupted by further recombination. Here we report on the variation unique to the cpp mutation, i.e. single-nucleotide variants and insertions or deletions. Although the candidate region contains several genes of interest with regard to the cpp phenotype, only one cpp-linked variant was predicted to have a significant physiological effect by causing a frameshift mutation in ESRP2, which has a role in tissue-specific splicing during development.


Assuntos
Galinhas , Fissura Palatina/veterinária , Mutação , Doenças das Aves Domésticas/genética , Animais , Fissura Palatina/genética , Fenótipo , Análise de Sequência de DNA/veterinária
16.
J Parasitol ; 106(2): 221-232, 2020 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-32164028

RESUMO

Members of the sucking louse genus Pedicinus are ectoparasites of cercopithecid primates in Africa, Asia, and Gibraltar. Pedicinus gabonensis n. sp. is described on the basis of adult male and female specimens collected from the mandrill (Mandrillus sphinx) in Gabon. The new species is compared morphologically with other members of the genus Pedicinus, and a nuclear elongation factor 1 alpha gene sequence is provided. Host associations and geographical distributions of the 18 previously recognized species of the genus and of P. gabonensis n. sp. are reviewed. Updated identification keys are provided for males and females of all known valid species of Pedicinus.


Assuntos
Anoplura/classificação , Infestações por Piolhos/veterinária , Mandrillus/parasitologia , Doenças dos Macacos/parasitologia , Animais , Anoplura/anatomia & histologia , Anoplura/genética , Anoplura/fisiologia , DNA/química , DNA/isolamento & purificação , Feminino , Gabão/epidemiologia , Infestações por Piolhos/epidemiologia , Infestações por Piolhos/parasitologia , Masculino , Doenças dos Macacos/epidemiologia , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterinária
18.
PLoS One ; 15(2): e0228483, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32027722

RESUMO

The aim of this study was to select a candidate deep-sea amphipod species suitable for connectivity analyses in areas around cobalt-rich ferromanganese crusts (CRCs). We applied DNA barcoding based on the mitochondrial protein-coding gene, cytochrome c oxidase subunit I (COI), to specimens collected from the Xufu Guyot (the JA06 Seamount) off southeastern Minami-Torishima Island in the North Pacific, where CRCs are distributed. We used baited traps to collect 37 specimens. Comparison of COI sequences with public reference databases (GenBank, BOLD) showed that almost all of the specimens belonged to the superfamily Lysianassoidea, which is known to be ubiquitous in deep-sea areas. In a molecular taxonomic analysis of these sequences, we detected 11 clades. One of these clades (group 9) composed of 18 sequences and was identified by DNA barcoding as a putative species belonging to Abyssorchomene, which has been reported from the New Hebrides Trench in the South Pacific. We considered this species to be a candidate for connectivity analysis and analyzed its genome by restriction site-associated DNA sequencing. The results showed that the genetic variation in this species is adequate for analyzing connectivity patterns in CRC areas in the future.


Assuntos
Anfípodes/classificação , Anfípodes/genética , Cobalto/química , Código de Barras de DNA Taxonômico/métodos , Sedimentos Geológicos/química , Ferro/química , Manganês/química , Animais , Variação Genética , Japão , Oceanos e Mares , Filogenia , Análise de Sequência de DNA/veterinária , Especificidade da Espécie , Vanuatu
19.
J Parasitol ; 106(1): 157-166, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32053468

RESUMO

This study provides additional taxonomic features based on scanning electron microscopy (SEM) and molecular data for Paracamallanus cyathopharynx ( Baylis, 1923 ) (Nematoda: Camallanidae). Parasites were collected from the posterior end of the intestine of cultured freshwater Sharptooth catfish Clarias gariepinus (Burchell, 1822) from Kibos fish farm, Kisumu County, Kenya, from December 2017 to March 2018. Additional taxonomic features recorded for P. cyathopharynx include the occurrence of 4-5 equal length digitate processes on the caudal end of the female, 4 processes (2 smaller and 2 larger) on the male caudal end, and a description of the shape of the distal tip of the right spicule. The study provides SEM images of the exposed sclerotized buccal capsule. This gives more information on the tridents, the sclerotized plate that extends laterally from the buccal capsule, and the narrow isthmus separating the anterior buccal capsule from the posterior. The prevalence, intensity, mean intensity, and mean abundance was 52.91%, 2-38, 12.37 and 6.54, respectively. 18S rDNA fragments were amplified, sequenced, and compared to other camallanid taxa, and 18S data confirmed the identity of the newly obtained sequences (MN396556) as that of P. cyathopharynx, being identical to sequence DQ813445 from Tanzania. This represents the first geographical record of P. cyathopharynx in Kenya.


Assuntos
Peixes-Gato/parasitologia , Doenças dos Peixes/parasitologia , Infecções por Spirurida/veterinária , Spirurina/genética , Spirurina/ultraestrutura , Animais , DNA Ribossômico/química , Feminino , Doenças dos Peixes/epidemiologia , Pesqueiros , Água Doce , Intestinos/parasitologia , Quênia/epidemiologia , Masculino , Microscopia Eletrônica de Varredura/veterinária , Prevalência , RNA Ribossômico 18S/genética , Alinhamento de Sequência/veterinária , Análise de Sequência de DNA/veterinária , Infecções por Spirurida/epidemiologia , Infecções por Spirurida/parasitologia
20.
Genome ; 63(3): 179-187, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31917611

RESUMO

Hair follicle (HF) growth and cycling is a complex biological process that occurs in most mammals. As HF growth and cycling directly impacts rabbit wool yield, it is important to better understand the potential regulation pattern of HF development. Our previous study demonstrated that HTATIP2 may participate in regulating rabbit HF cycles, but the molecular mechanism of HTATIP2 remained unclear. In this study, the coding sequence of the HTATIP2 gene in Angora rabbit was cloned. The length of the coding region sequence was 840 bp, which could code 279 amino acids, and exhibited high homology in different mammals. Bioinformatics analyses indicated that the HTATIP2 protein is stable, hydrophilic, located around the cytoplasm, and has a putative signal peptide. Moreover, we verified that HTATIP2 is highly expressed during catagen and telogen of the HF cycle. The overexpression vector was constructed and siRNAs were designed. Overexpression and knockdown of HTATIP2 appeared to regulate JAK-STAT pathway genes, such as BCL2, CCND1, c-Myc, and STAT2. It is therefore likely that HTATIP2 promotes cell apoptosis and inhibits cell proliferation. Our results indicate that HTATIP2 is highly expressed during catagen and telogen and may play an important role in JAK-STAT signaling. This study provides a theoretical foundation for investigating HTATIP2 in biological processes.


Assuntos
Acetiltransferases/genética , Acetiltransferases/metabolismo , Clonagem Molecular/métodos , Folículo Piloso/citologia , Animais , Apoptose , Linhagem Celular , Proliferação de Células , Folículo Piloso/metabolismo , Coelhos , Análise de Sequência de DNA/veterinária , Transdução de Sinais , Regulação para Cima ,
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