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1.
Urology ; 136: 150-151, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-32033667
2.
Urologe A ; 59(3): 284-288, 2020 Mar.
Artigo em Alemão | MEDLINE | ID: mdl-32047954

RESUMO

The prevalence of varicocele in children is less than 1% and in 11- to 19-year-old boys 8-14%. Up to 15% of patients with varicocele have impaired fertility. The indication for therapy of varicocele is still controversially discussed. In clinical trials testicular volume, volume difference, semen quality, fertility, hormones and pain have been investigated. The results are very heterogeneous for all parameters, so that the evidence level of recommendations for therapy of varicocele is very low. Nevertheless, the EAU guidelines recommend therapy of varicocele in children and young adults in case of small testis (volume difference >2 ml or 20%), additional cryptorchism, bilateral palpable varicoceles, symptomatic varicoceles and in case of pathological semen (in older boys). Microsurgical inguinal and laparoscopic procedures with preservation of lymph vessels have the best success and lowest complication rates.


Assuntos
Infertilidade Masculina/etiologia , Microcirurgia/métodos , Testículo/patologia , Varicocele/complicações , Varicocele/terapia , Adolescente , Adulto , Idoso , Criança , Criptorquidismo , Fertilidade , Humanos , Laparoscopia , Masculino , Análise do Sêmen , Resultado do Tratamento , Varicocele/diagnóstico , Adulto Jovem
3.
JAMA ; 323(1): 35-48, 2020 01 07.
Artigo em Inglês | MEDLINE | ID: mdl-31910279

RESUMO

Importance: Dietary supplements marketed for male fertility commonly contain folic acid and zinc based on limited prior evidence for improving semen quality. However, no large-scale trial has examined the efficacy of this therapy for improving semen quality or live birth. Objective: To determine the effect of daily folic acid and zinc supplementation on semen quality and live birth. Design, Setting, and Participants: The Folic Acid and Zinc Supplementation Trial was a multicenter randomized clinical trial. Couples (n = 2370; men aged ≥18 years and women aged 18-45 years) planning infertility treatment were enrolled at 4 US reproductive endocrinology and infertility care study centers between June 2013 and December 2017. The last 6-month study visit for semen collection occurred during August 2018, with chart abstraction of live birth and pregnancy information completed during April 2019. Interventions: Men were block randomized by study center and planned infertility treatment (in vitro fertilization, other treatment at a study site, and other treatment at an outside clinic) to receive either 5 mg of folic acid and 30 mg of elemental zinc (n = 1185) or placebo (n = 1185) daily for 6 months. Main Outcomes and Measures: The co-primary outcomes were live birth (resulting from pregnancies occurring within 9 months of randomization) and semen quality parameters (sperm concentration, motility, morphology, volume, DNA fragmentation, and total motile sperm count) at 6 months after randomization. Results: Among 2370 men who were randomized (mean age, 33 years), 1773 (75%) attended the final 6-month study visit. Live birth outcomes were available for all couples, and 1629 men (69%) had semen available for analysis at 6 months after randomization. Live birth was not significantly different between treatment groups (404 [34%] in the folic acid and zinc group and 416 [35%] in the placebo group; risk difference, -0.9% [95% CI, -4.7% to 2.8%]). Most of the semen quality parameters (sperm concentration, motility, morphology, volume, and total motile sperm count) were not significantly different between treatment groups at 6 months after randomization. A statistically significant increase in DNA fragmentation was observed with folic acid and zinc supplementation (mean of 29.7% for percentage of DNA fragmentation in the folic acid and zinc group and 27.2% in the placebo group; mean difference, 2.4% [95% CI, 0.5% to 4.4%]). Gastrointestinal symptoms were more common with folic acid and zinc supplementation compared with placebo (abdominal discomfort or pain: 66 [6%] vs 40 [3%], respectively; nausea: 50 [4%] vs 24 [2%]; and vomiting: 32 [3%] vs 17 [1%]). Conclusions and Relevance: Among a general population of couples seeking infertility treatment, the use of folic acid and zinc supplementation by male partners, compared with placebo, did not significantly improve semen quality or couples' live birth rates. These findings do not support the use of folic acid and zinc supplementation by male partners in the treatment of infertility. Trial Registration: ClinicalTrials.gov Identifier: NCT01857310.


Assuntos
Suplementos Nutricionais , Ácido Fólico/farmacologia , Infertilidade Masculina/tratamento farmacológico , Sêmen/efeitos dos fármacos , Zinco/farmacologia , Adolescente , Adulto , Fragmentação do DNA/efeitos dos fármacos , Suplementos Nutricionais/efeitos adversos , Feminino , Fertilização In Vitro , Ácido Fólico/efeitos adversos , Ácido Fólico/uso terapêutico , Humanos , Nascimento Vivo , Masculino , Pessoa de Meia-Idade , Análise do Sêmen , Contagem de Espermatozoides , Falha de Tratamento , Adulto Jovem , Zinco/efeitos adversos , Zinco/uso terapêutico
5.
Urology ; 136: 146-151, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31778681

RESUMO

OBJECTIVE: To ensure procedure success, American Urological Association Guidelines recommend postvasectomy semen analysis (PVSA); however, current literature suggests poor compliance. We sought to measure PVSA compliance and assess barriers to completion. METHODS: A retrospective review was performed of vasectomies at San Diego Veterans Administration Hospital and UC San Diego Health between 2006 and 2018. Patients received preprocedural counseling regarding semen analysis necessity. Postprocedural management included follow-up visit within 2-4 weeks and semen analysis after 15-20 ejaculations. Demographics and periprocedural variables were collected. Telephone interviews assessed patient reported reasons for noncompliance. Multivariable analysis was performed for factors associated with semen analysis. RESULTS: 503 men, mean age 38.8 years, underwent vasectomy at San Diego Veterans Administration Hospital (n = 331) and UC San Diego (n = 172). Overall, 80% completed clinical follow-up (n = 401) and 53% completed semen analysis (n = 268). The cohorts exhibited significantly different rates of semen analysis completion (46% vs 67%, P <.001) and clinical follow-up (64% vs 85%, P = .038). No difference was observed in age, fatherhood, or marital status. On multivariable analysis, fatherhood was the only factor associated with noncompliance of semen analysis (odds ratio 0.52, 95% confidence interval 0.33-0.83). Among men interviewed, the primary barriers to semen analysis completion were distance (38%), time constraints (34%), and forgetfulness (23%). Ninety-two% reported increased likelihood of completion with home-based semen testing. CONCLUSION: Patients demonstrated poor PVSA compliance despite preprocedural counseling. Given that distance and time constraints limited compliance, incorporating home-based semen testing may improve the quality of care for men undergoing vasectomy.


Assuntos
Acesso aos Serviços de Saúde/estatística & dados numéricos , Cooperação do Paciente/estatística & dados numéricos , Análise do Sêmen/estatística & dados numéricos , Vasectomia , Adulto , Humanos , Masculino , Período Pós-Operatório , Estudos Retrospectivos
7.
J Urol ; 203(1): 48-56, 2020 01.
Artigo em Inglês | MEDLINE | ID: mdl-31042452

RESUMO

PURPOSE: We evaluated the impact of varicocele grade on the response to varicocelectomy or spermatic vein embolization. MATERIALS AND METHODS: We systematically reviewed the published English language literature to identify studies on changes in semen quality and pregnancy outcomes after varicocele treatment, stratified by varicocele grade. Descriptive statistics and continuous random effects models were used to study the impact of varicocele grade and the surgical approach on the response to treatment. Result heterogeneity among studies was analyzed using the I2 statistic. Quality assessment of nonrandomized studies was done with the Newcastle-Ottawa Scale. Publication bias was analyzed using funnel plots and the Egger test. RESULTS: We identified 20 studies describing the outcome of varicocele treatment stratified by varicocele grade in a total of 2,001 infertile men with varicocele. A microsurgical approach (inguinal, subinguinal and/or Palomo) was used in 11 of the 20 studies (55%). Varicocele treatment was associated with improvements in sperm concentration and overall motility in patients with all grades of varicocele. Semen quality improvements were directly related to varicocele grade. The mean sperm concentration improvement in men with grades 1, 2, 2-3 and 3 varicoceles were 5.5, 8.9, 12.7 and 16.0 million sperm per ml, respectively. The mean improvement in the percent of overall motility in men with grades 1, 2, 2-3 and 3 varicoceles was 9.6%, 10.6%, 10.8% and 17.7%, respectively. Pregnancy outcomes were assessed but could not be analyzed systematically due to the lack of adequate published data. CONCLUSIONS: Mean improvements in the sperm concentration and the percent of overall motility after treatment of grade 1 varicocele were statistically significant but small in magnitude. In contrast, mean improvements in the sperm concentration and the percent of overall motility after treatment of grade 2-3 varicoceles were greater and highly likely to be clinically significant. Incorporating varicocele grade into shared decision making discussions with affected couples may improve the ability to select patients who are the best candidates for treatment.


Assuntos
Varicocele/cirurgia , Adulto , Feminino , Humanos , Infertilidade Masculina/etiologia , Masculino , Microcirurgia , Gravidez , Taxa de Gravidez , Análise do Sêmen , Varicocele/complicações
8.
J Dairy Sci ; 103(1): 545-555, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31668445

RESUMO

The aim of this study was to estimate genetic parameters and genetic trends for male fertility in Norwegian Red bulls. We analyzed data on semen characteristics traits collected at the performance test station of young bulls from 1994 to 2016, in an andrology test used to ensure acceptable semen quality before being selected as an artificial insemination bull. Traits included were volume, concentration, and motility (percentage of moving sperm cells) in fresh samples and after storing for 24 and 48 h, and sperm defects. The data consisted of 14,972 ejaculates from 3,927 young (11-15 mo) Norwegian Red bulls. Genetic parameters were estimated using bivariate linear animal models that included age in months, group-year, and collection-group (main effect of the interaction between ejaculate number and interval between collections) as fixed effects, and test-day and additive genetic and permanent environment effect of the bull as random effects. Considerable genetic coefficients of variation were found for concentration and volume, with lower values for motility. Estimated heritabilities ranged from 0.02 and 0.03 (for sperm defects and motility in fresh samples) to 0.14 (volume and concentration measured on a continuous scale). All estimated genetic correlations were favorable, but the genetic correlations between volume and concentration and volume and sperm defects were not significantly different from zero. The genetic correlations between concentration and motility traits ranged from 0.53 to 0.83, and those between volume and the motility traits were between 0.24 and 0.57. All traits showed a slightly unfavorable genetic trend. Our results indicate that selection of bulls with better sperm quality is possible.


Assuntos
Bovinos/genética , Análise do Sêmen/veterinária , Sêmen , Animais , Inseminação Artificial/veterinária , Masculino , Noruega , Motilidade Espermática , Espermatozoides
10.
Theriogenology ; 141: 9-15, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31479777

RESUMO

Artificial insemination (AI) has been used as a routine technology globally in the pig production industry since 1930. One of the preferable advantages of AI technology is that the semen of elite boars can be disseminated to the commercial sow population rapidly. Understanding the genetic background of semen traits may help in developing genetic improvement programs of boars by including these traits into the selection index. In this study, we utilized weighted single-step genome-wide association study (wssGWAS) to identify genetic regions and further candidate genes associated with sperm morphology abnormalities (proximal droplet, distal droplet, bent tail, coiled tail, and distal midpiece reflex) in a Duroc boar population. Several genomic regions explained 2.76%-9.22% of the genetic variances for sperm morphology abnormalities were identified. The first three detected QTL regions together explained about 7.65%-25.10% of the total genetic variances of the studied traits. Several genes were detected and considered as candidate genes for each of the traits under study: coiled tail, HOOK1, ARSA, SYCE3, SOD3, GMNN, RBPJ, STIL, and FGF1; bent tail, FGF1, ADIPOR1, ARPC5, FGFR3, PANX1, IZUMO1R, ANKRD49, and GAL; proximal droplet, NSF, WNT3, WNT9B, LYZL6, FGFR1OP, RNASET2, FYN, LRRC6, EPC1, DICER1, FNDC3A, and PFN1; distal droplet, ARSA, SYCE3, MOV10L1, CBR1, KDM6B, TP53, PTBP2, UBR7, KIF18A, ADAM15, FAAH, TEKT3, and SRD5A1; and distal midpiece reflex, OMA1, PFN1, PELP1, BMP2, GPR18, TM9SF2, and SPIN1. GO and KEGG enrichment analysis revealed the potential function of the identified candidate genes in spermatogenesis, testis functioning, and boar spermatozoa plasma membrane activating and maintenance. In conclusion, we detected candidate genes associated with the coiled tail, bent tail, proximal droplet, distal droplet, and distal midpiece reflex in a Duroc boar population using wssGWAS. Overall, these novel results reflect the polygenic genetic architecture of the studied sperm morphology abnormality traits, which may provide knowledge for conducting genomic selection on these traits. The detected genetic regions can be used in developing trait-specific marker assisted selection models by assigning higher genetic variances to these regions.


Assuntos
Estudo de Associação Genômica Ampla/veterinária , Espermatozoides/anormalidades , Suínos/genética , Animais , Regulação da Expressão Gênica , Variação Genética , Masculino , Análise do Sêmen/veterinária , Espermatogênese , Transcriptoma
11.
Theriogenology ; 141: 68-81, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31518731

RESUMO

The binding of seminal plasma (SP) proteins by spermatozoa plays an important role in the regulation of sperm epididymal maturation, motility gaining in female reproductive tracts and sperm-egg interaction. The aim of the study was to analyze the SP and sperm extracts proteome of cat (Felis catus) semen. The seminal plasma and spermatozoa were obtained by urethra catheterization from 10 male cats. Proteins were extracted using RIPA buffer and separated by electrophoresis (SDS-PAGE). The gels were analyzed using MultiAnalyst software. The proteins were subsequently analyzed using NanoUPLC-Q-TOF/MS. UniProt database-supported identification resulted in 106 proteins identified in the cat SP and 98 proteins in the extracts of spermatozoa. Based on a gene ontology analysis, dominant molecular functions of feline SP proteins were binding, catalytic, and antioxidant activity (56%, 33%, and 11% of cases, respectively). The molecular functions of sperm extracts proteins were mainly involved in catalytic activity (41%) and binding (23%). The proteins present in both, the SP and spermatozoa's extracts, were: serum albumin (ALB), semenogelin 2 (SEMG 2), clusterin (CLU), lactoferrin (LTF), prostatic acid phosphatase (ACPP), prolactin inducible protein (PIP), negative elongation factor E (NELF-E) and ectonucleotide pyrophosphatase (ENPP3). Protein-protein interactions analysis showed significant connection for 12 proteins in the cat semen. The seminal plasma proteins which, with high probability score, participate in important metabolic pathways are: glutathione peroxidases (GPx5 and 6), prostatic acid phosphatase (ACPP), ß-hexosaminidase (HEXB), polymeric immunoglobulin receptor (pIgR) and serpin family F member 1 (SERPINF1). For sperm protein extracts it were: pyruvate dehydrogenase (PDHB), succinate-CoA-ligase (SUCLA2), malate dehydrogenase (MDH2), ATP synthase F1 subunit alpha (ATP5F1A) and tubulin beta (TUBB).


Assuntos
Gatos , Proteoma/metabolismo , Análise do Sêmen/veterinária , Sêmen/fisiologia , Cateterismo Urinário/veterinária , Animais , Regulação da Expressão Gênica , Masculino , Transdução de Sinais , Espermatozoides/metabolismo
12.
Theriogenology ; 141: 161-167, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31542519

RESUMO

To test our hypothesis that antisperm antibodies (ASA) might alter sperm phenotypic attributes thus leading to sub-fertility/infertility in bulls, ASA were generated in crossbred male calves by immunizing with sperm two times. Cryopreserved spermatozoa from crossbred bulls (n = 24) with different field fertility ratings were incubated with ASA and different patterns of ASA immunolocalization were studied. In addition, sperm membrane integrity, acrosomal integrity and cryo-capacitation status were also assessed. Immunolocalization of sperm antigens using antisperm antibody revealed three major patterns (Acrosomal-AR, apical-AP and, acrosome and tail-AT). The proportion of ASA reactive spermatozoa was significantly (P < 0.05) higher in low- and medium-fertile bulls compared to high-fertile bulls. Among the three patterns, the proportion of spermatozoa with AR pattern was significantly (P < 0.05) higher in low- and medium-fertile bulls compared to high-fertile bulls. The proportion of membrane and acrosome intact spermatozoa was significantly (P < 0.05) higher in high-fertile bulls compared to medium- and low-fertile bulls. There were no significant differences in the proportion of cryo-capacitated spermatozoa among high-, medium- and low-fertile bulls. The relationship between ASA reactive spermatozoa and conception rates (CR) of bulls was highly (P < 0.01) significant and negative. Similarly, AR and AT pattern were also significantly (P < 0.01) and negatively related to CR of bulls. The reactivity of spermatozoa with ASA was also significantly (P < 0.01) and negatively related to the membrane and acrosome integrity of spermatozoa. It was concluded that the proportion of spermatozoa responding to ASA was higher in low-compared to high-fertile bulls and ASA localization in sperm acrosomal area was negatively related to sperm membrane and acrosomal integrity and bull fertility.


Assuntos
Anticorpos/fisiologia , Especificidade de Anticorpos/fisiologia , Bovinos/fisiologia , Fertilidade/fisiologia , Espermatozoides/fisiologia , Animais , Antígenos/fisiologia , Bovinos/imunologia , Masculino , Análise do Sêmen
13.
Ecotoxicol Environ Saf ; 190: 110044, 2020 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-31869717

RESUMO

Low-energy shock waves (LESWs) have been widely used in the intervention of a subset of diseased tissues and organs with good results. However, it is unclear whether they can be used directly to intervene in the testes. Therefore, the aim of this study was to determine a relatively safe energy density and impulse number for rat testes. A total of 176 male rats were randomly and equally assigned to different intervention groups. Among them, 144 rats were assigned to 18 shock subgroups with different energy densities (0.02, 0.04 and 0.06 mJ/mm2), different impulse numbers (500, 1000 and 1500 impulses) and different shock periods (2 and 8 weeks). The remaining 32 rats were divided into the sham intervention (S) groups and the blank control (N) groups with observation periods of 2 weeks and 8 weeks. One day after the last LESWs intervention, all the rats were weighed, and the concentrations of reproductive endocrine hormones were measured, the semen quality and testicular tissue oxidative stress levels were analyzed, and histomorphology and ultrastructures were observed. We found that there were no significant differences in the whole-body physiological state, testicular tissue morphology, oxidative stress state and sperm quality between the L1 shock group and the corresponding S group and N group (all p˃0.05, respectively). However, the other parameters of the shock groups caused different degrees of damage to the structure and function of rat testes, and the whole-body physiological state was also adversely affected. This study demonstrated that LESWs with an energy density of 0.02 mJ/mm2 and 500 impulses had no adverse effects on the rat testes.


Assuntos
Testículo/efeitos da radiação , Animais , Masculino , Estresse Oxidativo , Ratos , Análise do Sêmen , Espermatozoides/efeitos da radiação , Testículo/anatomia & histologia , Testículo/química , Testículo/ultraestrutura
14.
Environ Health Perspect ; 127(12): 127005, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31841032

RESUMO

BACKGROUND: Epidemiological evidence remains equivocal on the associations between environmentally relevant levels of per-/polyfluoroalkyl substances (PFASs) and human semen quality. OBJECTIVES: We aimed to test whether the potential effects on semen quality could be better observed when seminal PFAS levels were used as an exposure marker compared with serum PFAS levels. METHODS: Matched semen and serum samples from 664 adult men were collected from a cross-sectional population in China from 2015 to 2016. Multiple semen parameters were assessed, along with measurement of 16 target PFASs in semen and serum. Partitioning between semen and serum was evaluated by the ratio of matrix-specific PFAS concentrations. Regression model results were expressed as the difference in each semen parameter associated with the per unit increase in the ln-transformed PFAS level after adjusting for confounders. RESULTS: Perfluorooctanoate (PFOA), perfluorooctane sulfonate (PFOS), and emerging chlorinated polyfluorinated ether sulfonate (6:2 Cl-PFESA) were detected at their highest concentrations in both semen and serum, with median concentrations of 0.23, 0.10, and 0.06 ng/mL in semen, respectively, and a semen-to-serum ratio of 1.3:3.1. The between-matrix correlations of these PFAS concentrations were high (R=0.70-0.83). Seminal PFOA, PFOS, and 6:2 Cl-PFESA levels were significantly associated with a lower percentage of progressive sperm and higher percentage of DNA fragmentation (false discovery rate-adjusted p-values of<0.05). Associations between serum PFAS levels and semen parameters were generally statistically weaker, except for DNA stainability, which was more strongly associated with serum-based PFASs than with semen-based PFASs. CONCLUSIONS: Our results suggest the potential for deleterious effects following exposure to 6:2 Cl-PFESA and other PFASs. Compared with serum PFAS levels, the much clearer association of seminal PFAS levels with semen parameters suggests its advantage in hazard assessment on semen quality, although the potential for confounding might be higher. Exposure measurements in target tissue may be critical in clarifying effects related to PFAS exposure. https://doi.org/10.1289/EHP4431.


Assuntos
Exposição Ambiental/estatística & dados numéricos , Poluentes Ambientais/metabolismo , Fluorcarbonetos/metabolismo , Sêmen/metabolismo , Adulto , Ácidos Alcanossulfônicos , Caprilatos , Poluentes Ambientais/sangue , Humanos , Masculino , Análise do Sêmen
15.
Clín. investig. ginecol. obstet. (Ed. impr.) ; 46(4): 143-147, oct.-dic. 2019. graf
Artigo em Espanhol | IBECS | ID: ibc-184301

RESUMO

Introducción: La fertilidad y la virilidad suelen relacionarse socialmente con el concepto de masculinidad; esto genera cierto grado de incertidumbre debido a que podría conllevar una serie de procesos emocionales al relacionar los conceptos anteriores con la palabra infertilidad. Objetivo: El objetivo del presente trabajo fue relacionar las respuestas sobre las percepciones de la calidad seminal con algunos conceptos relacionados con la masculinidad. Materiales y métodos: Se llevó a cabo una encuesta virtual anónima a 500 hombres en la cual se realizaron preguntas con relación a la percepción de algunas variables sobre la calidad seminal. Resultados: Respecto a la pregunta ¿para usted qué significa tener un conteo alto de espermatozoides?, en hombres con y sin pareja la respuesta más frecuente fue buena capacidad para fecundar, siendo mayor en hombres con pareja (p = 0,013); en contraste, la segunda respuesta más frecuente (buena salud) fue más alta en el grupo que no tenía pareja (p = 0,028), mientras que la mayoría de los participantes respondieron que no sentirían nada (58,8% sin pareja y 64,5% con pareja) frente a las preguntas ¿qué sentiría si tiene un conteo bajo de espermatozoides? y ¿qué sentiría si no tiene ningún espermatozoide?, respectivamente. Por otro lado, en cuanto a la respuesta «avergonzado», los participantes sin pareja decían sentirse más avergonzados en ambas preguntas (p = 0,011 y p = 0,0057). Conclusión: El termino infertilidad debería ser usado cuidadosamente, pues es de gran impacto en la vida del individuo, afecta tanto el bienestar de la pareja como el bienestar psicosocial y la propia satisfacción sexual


Introduction: Fertility and virility are often socially related to the concept of masculinity, and this generates a certain degree of uncertainty because it could lead to a series of emotional processes by relating these concepts with the word infertility. Objective: The objective of the present work was to relate the responses on the perceptions of seminal quality with some concepts related to masculinity. Materials and methods: An anonymous virtual survey of 500 men was carried out, in which questions were asked regarding the perception of some variables on seminal quality. Results: As regards the question, 'what does it mean for you to have a high sperm count?'; in men with and without partners, the most frequent response was, good ability to fecundate, being higher in men with a partner (P = .013). In contrast, the second most frequent response (good health) was higher in the group that had no partner (P = .028). While the majority of participants answered that they would not feel anything (58.8% without a partner, and 64.5% with a partner) in response to the questions, 'How would you feel if you have a low sperm count?', and 'How would you feel if you did not have any sperm?', respectively. On the other hand, as regards the response 'embarrassed', the participants without a partner said they felt more embarrassed in both questions (P = .011 and P = .0057). Conclusion: The term infertility should be used carefully, since it has a significant impact an individual's life. It affects the well-being of the couple, as well as psychosocial well-being and sexual satisfaction itself


Assuntos
Humanos , Masculino , Fertilidade/fisiologia , Análise do Sêmen , Comportamento Sexual/estatística & dados numéricos , Infertilidade Masculina/epidemiologia , Masculinidade , Coito/fisiologia , Contagem de Espermatozoides/estatística & dados numéricos , Colômbia/epidemiologia
16.
Clín. investig. ginecol. obstet. (Ed. impr.) ; 46(4): 148-153, oct.-dic. 2019. tab
Artigo em Espanhol | IBECS | ID: ibc-184302

RESUMO

Introducción: La solicitud de semen donado ha aumentado en los últimos años, lo que ha llevado a evaluar con mayor rigurosidad las muestras de semen de los donantes. Sin embargo, estas muestras presentan una variabilidad debido a su biología, a la abstinencia sexual, a la actividad de las glándulas accesorias, a los errores analíticos, entre otros. Por lo tanto, el objetivo de este estudio fue evaluar la variación de los parámetros seminales de 15 voluntarios pertenecientes a un programa de donación del Centro de Medicina Reproductiva CONCEVIDAS. Materiales y métodos: Se analizaron los parámetros seminales convencionales de 384 muestras de semen provenientes de 15 donantes. Resultados: Se encontró una mayor variabilidad tanto intra como interindividuo en la concentración total de espermatozoides, en contraste, la viabilidad y la movilidad progresiva fueron los parámetros con menor variación. Conclusión: Este trabajo reafirma la variabilidad biológica del análisis seminal convencional en un grupo de individuos aparentemente sanos


Introduction: The request for donated semen has increased in recent years, which has led to a more rigorous evaluation of donor semen samples. However, the parameters of these samples vary due to their biology, sexual abstinence, the activity of accessory glands, and analytical errors, among others. Therefore, the objective of this study was to evaluate the variation in the seminal parameters of 15 volunteers of a sperm donation program from the reproductive medicine centre CONCEVIDAS. Materials and methods: An analysis was performed on the conventional seminal parameters of 384 semen samples from 15 donors. Results: A wide variation was found in the total sperm concentration. In contrast, viability and sperm progressive motility were the parameters with the least variation. Conclusion: This work confirms the biological variability of conventional seminal analysis in a group of apparently healthy individuals


Assuntos
Humanos , Masculino , Adulto , Sêmen/fisiologia , Análise do Sêmen/estatística & dados numéricos , Espermatozoides/fisiologia , Motilidade Espermática/fisiologia , Técnicas Reprodutivas/estatística & dados numéricos , Sêmen/metabolismo , Fertilização In Vitro
17.
18.
Medicine (Baltimore) ; 98(41): e17494, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31593115

RESUMO

BACKGROUND: Infertility has troubled the world's 186 million people, and male infertility accounts for more than half. The literature of physical exercise related to semen quality has shown inconsistent results, and there is currently no systematic review to evaluate the effects of exercise on reproductive outcomes in male infertility patients. This study aims to assessing the effects of exercise interventions based on randomized controlled trials (RCTs) on semen quality and reproductive outcomes in male infertility. METHODS: English and Chinese literature about physical exercise treatment for male infertility published before July 31, 2019 will be systematic searched in PubMed, Embase, Web of Science, Cochrane Library, Open Grey, Clinicaltrials.gov, Chinese Clinical Trial Registry, WANFANG, VIP Chinese Science and Technology Journal Database, CNKI, Chinese biomedical document service system (SinoMed). Only RCTs of patients with male infertility will be included. Literature screening, data extraction, and the assessment of risk of bias will be independently conducted by 2 reviewers, and the 3rd reviewer will be consulted if any different opinions existed. Live-birth rate, pregnancy rate, adverse events (including miscarriage), sperm concentration, progressive motility, sperm morphology, and sperm DNA fragmentation will be evaluated. Systematic review and meta-analysis will be produced by RevMan 5.3 and Stata 14.0. This protocol reported in accordance with the Preferred Reporting Items for Systematic Review and Meta-analysis Protocols (PRISMA-P) statement, and we will report the systematic review by following the PRISMA statement. CONCLUSION AND DISSEMINATION: We will assess the efficacy and safety of physical exercise on semen quality and reproductive outcomes in infertile men. The findings will be published in a peer-reviewed journal to provide evidence-based medical evidence for clinical decision making and the patient's lifestyle guidance. REGISTRATION INFORMATION: PROSPERO CRD42019140294.


Assuntos
Exercício/fisiologia , Infertilidade Masculina/epidemiologia , Reprodução/fisiologia , Espermatozoides/citologia , Adolescente , Adulto , Fragmentação do DNA , Feminino , Humanos , Nascimento Vivo/epidemiologia , Masculino , Pessoa de Meia-Idade , Gravidez , Taxa de Gravidez/tendências , Ensaios Clínicos Controlados Aleatórios como Assunto , Análise do Sêmen/estatística & dados numéricos , Contagem de Espermatozoides/estatística & dados numéricos , Espermatozoides/metabolismo , Adulto Jovem
19.
Environ Sci Pollut Res Int ; 26(33): 34575-34583, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31650475

RESUMO

The aim of the present study was to investigate whether curcumin (CUR) can ameliorate cadmium-induced reproductive toxicity and its mechanism. A total of 48 male mice were equally divided into 4 groups: control, CdCl2 (2 mg/kg, intraperitoneally inject) curcumin (50 mg/kg, intraperitoneally inject), co-treatment with curcumin (50 mg/kg), and CdCl2 (2 mg/kg) for 10 days. The results demonstrated that CdCl2 reduces sperm motility, decreases the sperm density and serum testosterone content, and significantly improves the rate of sperm deformity. CdCl2 increased the level of testicular total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activity, and glutathione (GSH), and CdCl2 declined the level of malondialdehyde (MDA). However, the semen quality of the mice in the curcumin intervention group was improved. Moreover, the testosterone content and antioxidant capacity were increased. In the Cd group mice, the expression of testicular Nrf2, as well as the mRNA and protein expressions of the downstream target molecules, glutathione peroxidase (GSH-Px), and γ-glutamylcysteine synthetase (γ-GCS) of Nrf2 declined, while the above genetic expressions elevated significantly in the curcumin intervention group. Our results suggested that curcumin could protect against Cd-induced testicular injury via activating the Nrf2/ARE signaling pathway.


Assuntos
Antioxidantes/farmacologia , Cádmio/toxicidade , Curcumina/farmacologia , Testículo/fisiologia , Animais , Antioxidantes/metabolismo , Fator de Transcrição de Proteínas de Ligação GA/metabolismo , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Fator 2 Relacionado a NF-E2/metabolismo , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Análise do Sêmen , Transdução de Sinais/efeitos dos fármacos , Motilidade Espermática/efeitos dos fármacos , Superóxido Dismutase/metabolismo , Testículo/efeitos dos fármacos , Testículo/metabolismo , Testosterona/metabolismo
20.
BMC Vet Res ; 15(1): 355, 2019 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-31640772

RESUMO

BACKGROUND: Different parameters are assessed as part of the semen analysis but a standard protocol for evaluation of goat semen is still missing. The aim of this study was to analyse two different factors affecting buck sperm quality in the post-collection period prior to adding the extender. Here we examined the effects of two handling temperatures (20 °C, 37 °C) and various examination time points (3-30 min) after semen collection. RESULTS: Examination time point had a significant influence on raw sperm viability (p < 0.05), motility (p < 0.05) and on semen pH (p < 0.05). The two different handling temperatures had no significant effect on sperm viability (p > 0.05), motility (p > 0.05), with the exception of fast moving sperm (p = 0.04), or on semen pH (p > 0.05). CONCLUSION: Examination time point was identified as factor strongly influencing raw peacock buck semen after collection. Raw goat semen can tolerate room temperatures for at least 10 min without impacting overall semen quality. In order to obtain comparable results, semen samples should always be examined within 10 min after collection.


Assuntos
Cabras , Análise do Sêmen/veterinária , Recuperação Espermática/veterinária , Espermatozoides/fisiologia , Animais , Masculino , Temperatura Ambiente , Fatores de Tempo
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