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1.
Ecotoxicol Environ Saf ; 214: 112094, 2021 May.
Artigo em Inglês | MEDLINE | ID: mdl-33677382

RESUMO

Honey has multifaceted nutritional and medicinal values; however, its quality is hinged on the floral origin of the nectar. Taking advantage of the large areas that they cover; honeybees are often used as bioindicators of environmental contamination. The focus of the present paper was to examine the quality of honey from within the vicinity of an abandoned pesticide store in Masindi District in western Uganda. Surficial soils (<20 cm depths) and honey samples were collected from within the vicinity of the abandoned pesticide store and analysed for organochlorine pesticide (OCP) residues using gas chromatograph coupled to an electron capture detector (GC-ECD). The mean level of ∑DDTs in all the soil samples was 503.6 µg/kg dry weight (d.w). ∑DDTs contributed 92.2% to the ∑OCPs contamination loads in the soil samples, and others (lindane, aldrin, dieldrin, and endosulfans) contributed only 7.8%. Ratio (p, p'-DDE+p, p'-DDD)/p, p'-DDT of 1.54 suggested historical DDT input in the area. In all the honey samples, the mean level of ∑DDTs was 20.9 µg/kg. ∑DDTs contributed 43.3% to ∑OCPs contamination loads in the honey samples, followed by lindane (29.8%), endosulfans (23.6%) and dieldrin (3.2%), with corresponding mean levels of 14.4, 11.4 and 1.55 µg/kg, respectively. Reproductive risk assessment was done based on the hazard quotient (HQ) and hazard index (HI) procedure. In our study, the calculated HIs for adults (102.38), and children (90.33) suggested high potential health risks to the honey consumers. Lindane, endosulfan and p, p'-DDD detected in the honey samples at levels exceeding the acute reference dose (ARfD) are known risk factors for spontaneous abortion, reduced implantation, menstrual cycle shortening, impaired semen quality, and prostate cancer in exposed individuals and experimental animal models.


Assuntos
Exposição Dietética/estatística & dados numéricos , Mel/análise , Hidrocarbonetos Clorados/análise , Resíduos de Praguicidas/análise , Adulto , Aldrina/análise , Animais , Criança , DDT/análise , Diclorodifenil Dicloroetileno/análise , Dieldrin/análise , Endossulfano/análise , Biomarcadores Ambientais , Monitoramento Ambiental/métodos , Feminino , Hexaclorocicloexano/análise , Humanos , Masculino , Praguicidas/análise , Saúde Reprodutiva , Análise do Sêmen , Solo , Uganda
2.
Adv Exp Med Biol ; 1261: 249-259, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33783748

RESUMO

Astaxanthin is known as a "marine carotenoid" and occurs in a wide variety of living organisms such as salmon, shrimp, crab, and red snapper. Astaxanthin antioxidant activity has been reported to be more than 100 times greater than that of vitamin E against lipid peroxidation and approximately 550 times more potent than that of vitamin E for singlet oxygen quenching. Astaxanthin doesn't exhibit any pro-oxidant nature and its main site of action is on/in the cell membrane. To date, extensive important benefits suggested for human health include anti-inflammation, immunomodulation, anti-stress, LDL cholesterol oxidation suppression, enhanced skin health, improved semen quality, attenuation of common fatigue including eye fatigue, increased sports performance and endurance, limiting exercised-induced muscle damage, and the suppression of the development of lifestyle-related diseases such as obesity, atherosclerosis, diabetes, hyperlipidemia, and hypertension. Recently, there has been an explosive increase worldwide in both the research and demand for natural astaxanthin mainly extracted from the microalgae, Haematococcus pluvialis, in human health applications. Japanese clinicians are especially using the natural astaxanthin as add-on supplementation for patients who are unsatisfied with conventional medications or cannot take other medications due to serious symptoms. For example, in heart failure or overactive bladder patients, astaxanthin treatment enhances patient's daily activity levels and QOL. Other ongoing clinical trials and case studies are examining chronic diseases such as non-alcoholic steatohepatitis, diabetes, diabetic nephropathy, and CVD, as well as infertility, atopic dermatitis, androgenetic alopecia, ulcerative colitis, and sarcopenia. In the near future, astaxanthin may secure a firm and signature position as medical food.


Assuntos
Microalgas , Análise do Sêmen , Humanos , Masculino , Qualidade de Vida , Xantofilas
3.
Methods Mol Biol ; 2218: 29-35, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33606220

RESUMO

Fish sperm show many measurable parameters which react sensitively in a dose- and time-dependent way to toxic exposure. Fish sperm is therefore used as an in vitro toxicology test system. One of the most sensitive and easily detectable parameters is progressive motility which can be measured by a computer-assisted sperm analysis (CASA) system. Here we describe a simple protocol to test the effect of environmental toxicants by using zebrafish (Danio rerio) sperm.


Assuntos
Substâncias Perigosas/toxicidade , Processamento de Imagem Assistida por Computador/métodos , Análise do Sêmen/métodos , Espermatozoides/fisiologia , Peixe-Zebra/fisiologia , Animais , Computadores , Masculino , Motilidade Espermática/efeitos dos fármacos , Motilidade Espermática/fisiologia , Espermatozoides/efeitos dos fármacos
4.
Eur J Endocrinol ; 184(4): 487-501, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33524003

RESUMO

Objective: To study the impact of the quality of therapeutic control on fertility and on the prevalence of testicular adrenal rest tumours (TART) in young males with congenital adrenal hyperplasia (CAH). Design: Combined cross-sectional and retrospective clinical study. Methods: Twenty-nine patients and age-matched controls underwent clinical investigation, including semen analysis, testicular and adrenal ultrasound imaging, and serum and hair steroid analysis. The quality of therapeutic control was categorized as 'poor', 'moderate' or 'medium'. Evaluation of current control was based on concentrations of 17-hydroxy-progesterone and androstenedione in serum and 3 cm hair; previous control was categorized based on serum 17-hydroxy-progesterone concentrations during childhood and puberty, anthropometric and puberty data, bone age data and adrenal sizes. Results: Semen quality was similar in males with CAH and controls (P = 0.066), however patients with 'poor' past control and large TART, or with 'poor' current CAH control had low sperm counts. Follicle-stimulating hormone was decreased, if current CAH control was 'poor' (1.8 ± 0.9 U/L; 'good': 3.9 ± 2.2 U/L); P = 0.015); luteinizing hormone was decreased if it was 'poor' (1.8 ± 0.9 U/L; P = 0.041) or 'moderate' (1.9 ± 0.6 U/L; 'good': 3.0 ± 1.3 U/L; P = 0.025). None of the males with 'good' past CAH control, 50% of those with 'moderate' past control and 80% with 'poor past control had bilateral TART. The prevalence of TART in males with severe (class null or A) CYP21A2 mutations was 53% and 25% and 0% in those with milder class B and C mutations, respectively. Conclusions: TART development is favoured by inadequate long-term hormonal control in CAH. Reduced semen quality may be associated with large TART. Gonadotropin suppression by adrenal androgen excess during the latest spermatogenic cycle may contribute to impairment of spermatogenesis.


Assuntos
Corticosteroides/uso terapêutico , Hiperplasia Suprarrenal Congênita/tratamento farmacológico , Tumor de Resto Suprarrenal/epidemiologia , Terapia de Reposição Hormonal/métodos , Análise do Sêmen , Neoplasias Testiculares/epidemiologia , Adolescente , Glândulas Suprarrenais/patologia , Hiperplasia Suprarrenal Congênita/genética , Hiperplasia Suprarrenal Congênita/fisiopatologia , Tumor de Resto Suprarrenal/patologia , Adulto , Androgênios/sangue , Humanos , Estudos Longitudinais , Masculino , Mutação , Puberdade , Espermatogênese , Neoplasias Testiculares/patologia , Ultrassonografia , Adulto Jovem
5.
Animal ; 15(2): 100065, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-33573944

RESUMO

Sperm quality traits routinely collected by artificial insemination (AI) center for rams progeny test are related with the capacity to produce sperm doses for AI and, in more or less grade, with males' fertility. Low-quality ejaculates are unuseful to perform AI sperm doses, which suppose high economic loses for the AI center. Moreover, sperm quality traits have low heritability values which make traditional genetic selection little efficient to its improvement. In this work, a genome-wide association study (GWAS) was conducted by using sperm quality traits data and 50 K Affymetrix custom chip genotypes of 429 rams of Assaf breed from OVIGEN AI centre. Furthermore, 47 of these rams were also genotyped with the Illumina HD Ovine BeadChip, and therefore HD genotypes were imputed for all rams with phenotype data. Previous to the GWAS, a linear regression model was fitted including sperm traits as dependent variables; the flock of origin, date of sperm collection, and jump number as fixed effects; rams age at collection in months as covariate; and ram permanent effect as random. Pseudo-phenotypes obtained from this model were used as input for GWAS. Associations at the chromosome-wise level (FDR 10%) of 76 single-nucleotide polymorphisms (SNPs) in 4 chromosomes for ejaculate concentration (CON), 20 SNPs in 3 chromosomes for ejaculate volume (VOL), 32 SNPs in 1 chromosome for ejaculate number of spermatozoa (SPZ), and 23 SNPs for spermatozoa mass motility (MOT) in 17 chromosomes were found. Only SNPs associated with MOT overcame the genome-wide significance level. Some candidate genes for sperm traits variability were SLC9C1 (OAR1), TSN (OAR2), and FUT10 (OAR26) for MOT;. DOCK2, CPLANE1, SPEF2, and RAI14 (OAR16) for CON; SCAPER and PSMA4 (OAR18) for VOL; and PARM1 and LOC101110593 (OAR6) for SPZ. SNPs associated with sperm traits were not found to be correlated with milk production genetic variation; however, the high frequencies of some SNPs with negative effect over sperm traits found in animals at the top milk yield estimated breeding values (EBVs) ranking would allow to exert some selective presure to improve rams sperm performances. Effects and frequencies of some of the SNPs detected over sperm quality traits make these variants good candidates to be used in marker-assisted selection to improve sperm characteristics of Assaf rams and AI center efficiency to produce sperm doses.


Assuntos
Estudo de Associação Genômica Ampla , Espermatozoides , Animais , Estudo de Associação Genômica Ampla/veterinária , Masculino , Fenótipo , Análise do Sêmen/veterinária , Ovinos/genética , Motilidade Espermática/genética
6.
Medicine (Baltimore) ; 100(4): e23350, 2021 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-33530158

RESUMO

INTRODUCTION: Infertility has affected millions of couples aged 15 to 44 years worldwide. Recently, some studies suggest that abnormal semen quality is the main cause of male infertility and asthenozoospermia accounts for 19% of the infertility of men. The situation has brought a huge burden to the patient with asthenozoospermia and society. Acupuncture is a part of traditional Chinese medicine. Electroacupuncture (EA) has gained in popularity. Although a positive effect of manual acupuncture and EA on sperm parameters has been documented in several studies, there still a lack of more solid evidence. We hope to provide a convincing study for EA. METHODS AND ANALYSIS: The electronic databases of MEDLINE, PubMed, Web of Science, EMBASE, Cochrane Library, Clinicaltrials. org, China National Knowledge Infrastructure Database (CNKI), Wan fang Database, China Biology Medicine Database (CBM), VIP Science Technology Periodical Database, Chinese Clinical Trial Registry will be retrieved. All the randomized controlled trials of rESWT for patients with CP/CPPS will be included. We will evaluate the outcomes including NIH-CPSI, VAS, IPSS, IIEF-5, and conduct this study strictly according to the Cochrane Handbook for Systematic Reviews of Interventions. RESULTS: The present study is a protocol for systematic review and meta-analysis without results, and data analysis will be carried out after the protocol. We will share our findings on October 31st, 2021. CONCLUSIONS: EA for asthenospermia is a microtrauma surgery with less pain. EA can effectively improve sperm motility; however, its efficacy has not been assessed scientifically and systematically. To address this limitation, this study will inspect the efficacy and safety of the EA in patients with asthenospermia. ETHICS AND DISSEMINATION: Formal ethical approval is not required in this protocol. We will collect and analyze data based on published studies, and since there are no patients involved in this study, individual privacy will not be under concerns. The results of this review will be disseminated to peer-reviewed journals or submit to related conferences. PROTOCOL REGISTRATION NUMBER: INPLASY2020100071.


Assuntos
Astenozoospermia/terapia , Eletroacupuntura/métodos , Adolescente , Adulto , Humanos , Masculino , Metanálise como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto , Projetos de Pesquisa , Análise do Sêmen , Revisões Sistemáticas como Assunto , Resultado do Tratamento , Adulto Jovem
7.
Reproduction ; 161(3): 319-331, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33522983

RESUMO

The existing evidence suggests that the human reproductive system may be potentially vulnerable to COVID-19 infection. However, little is known about the virus-host interaction of COVID-19 in sperm cells. We are the first to address the connection between changes in multiple seminal biomarkers and reproductive function in male patients recovering from COVID-19. In a prospective longitudinal cohort study, seminal ACE2 activity, markers of inflammation and oxidative stress, apoptotic variables, and semen quality parameters were evaluated at 10-day intervals for a maximum follow-up time of 60 days among male patients with laboratory-confirmed COVID-19 (n = 84) and healthy controls (CON; n = 105). At the baseline and the subsequent follow-ups, the COVID-19 group revealed significantly higher levels of seminal plasma ACE2 enzymatic activity, IL-1ß, IL-6, IL-8, IL-10, TGF-ß, TNF-α, IFN-α, IFN-γ, ROS, caspase-8, caspase-9, and caspase-3 activity as well as lower levels of SOD activity than those in the CON group (P < 0.05). These perturbations tended to persist over time and were correlated with significant impairments in semen volume, progressive motility, sperm morphology, sperm concentration, and the number of spermatozoa. We provide the direct experimental evidence that the male reproductive system could be targeted and damaged by the COVID-19 infection. These findings go beyond our current understanding of the disease, suggesting that the reproductive function of the patients recovering from the disease should be precisely followed and evaluated to detect and avoid more serious reproductive problems in the future, as they may develop a transient state of male subfertility like those with oligoasthenoteratozoospermia.


Assuntos
/metabolismo , Infertilidade Masculina/virologia , Sêmen/virologia , Espermatozoides/virologia , Adulto , Biomarcadores/metabolismo , Citocinas/metabolismo , Fertilidade/fisiologia , Humanos , Infertilidade Masculina/metabolismo , Masculino , Estresse Oxidativo/fisiologia , Estudos Prospectivos , Espécies Reativas de Oxigênio/metabolismo , Sêmen/metabolismo , Análise do Sêmen , Contagem de Espermatozoides , Motilidade Espermática/fisiologia , Espermatozoides/metabolismo , Adulto Jovem
8.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi ; 38(2): 188-193, 2021 Feb 10.
Artigo em Chinês | MEDLINE | ID: mdl-33565079

RESUMO

DNA methylation as an important aspect of epigenetics plays an important role in spermatogenesis and embryonic development. In recent years, researchers have found that male infertility, in particular abnormal semen quality, is related to abnormal DNA methylation. To further delineate the pathogenesis of male infertility and inspire new ideas for the treatment of male infertility, a comprehensive review over the correlation between abnormal methylation of imprinted genes, repetitive DNA elements and non-imprinted genes, semen quality (including sperm count, morphology, and vitality) and male infertility is provided.


Assuntos
Metilação de DNA , Infertilidade Masculina , Espermatozoides/patologia , Humanos , Infertilidade Masculina/genética , Masculino , Análise do Sêmen , Contagem de Espermatozoides , Espermatogênese
9.
Trop Anim Health Prod ; 53(1): 61, 2021 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-33389175

RESUMO

This study was designed to evaluate the seasonal expression of seminal plasma proteins from two bovine breeds adapted to a subtropical climate and their associations with post-thawing sperm and environmental characteristics. Semen samples were obtained three times in summer and three times in winter from four Crioulo Lageano and four Angus bulls. Seminal plasma was obtained by centrifugation, and the other portion of the semen was cryopreserved. Seminal plasma proteins were identified by 2D-nanoUPLC-MSE. Post-thawing assessments of sperm kinetics, morphology and membrane integrity were performed. Environmental data such as air temperature, air humidity and black globe temperature (BGT) were recorded, and the temperature-humidity index (THI) was calculated in summer and winter. Results showed that the climate varied significantly between seasons. Although no statistical differences were observed in semen quality between breeds, the protein profiles varied within and between seasons. We suggest that the most critical proteins in summer affecting sperm characteristics were TIMP-2, DNase, Clusterin, CFAH and GPx6. TIMP-2 and DNase showed a higher abundance in Crioulo Lageano in comparison with Angus, while Clusterin, CFAH and GPx6 presented a lower abundance. To the best of our knowledge, this is the first report of a recently evolved type of glutathione peroxidase, GPx6, in seminal plasma of bovines. In winter, five proteins were considered to be more critical: BSP1, BSP3, CCL2, Sulfhydryl oxidase and TIMP-2. BSP1 and TIMP-2 showed a lower abundance while BSP3, CCL2 and Sulfhydryl oxidase presented a higher abundance in this season in Crioulo Lageano in comparison with Angus.


Assuntos
Aclimatação , Bovinos/metabolismo , Estações do Ano , Proteínas de Plasma Seminal/metabolismo , Adaptação Fisiológica , Animais , Cruzamento , Criopreservação/veterinária , Umidade , Masculino , Mapas de Interação de Proteínas , Sêmen , Análise do Sêmen/veterinária , Espermatozoides , Temperatura
10.
Animal ; 15(1): 100001, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33515997

RESUMO

Contamination of semen with urine and asynchronous maturation of males and females are main obstacles in artificial reproduction of pikeperch Sander lucioperca. The objective of this study was to overcome these obstacles using optimization of a procedure for short-term storage of pikeperch semen at 4°C using two immobilizing media (IM): (a) IM1, 180mM NaCl, 2.68mM KCl, 1.36mM CaCl2⋅2H2O and 2.38mM NaHCO3, 343mOsm/kg; and (b) IM2, 200mM NaCl, 2.68mM KCl, 1.36mM CaCl2⋅2H2O and 2.38mM NaHCO3, 381mOsm/kg. Undiluted sperm was used as the control. At 6h poststorage, there were no substantial changes in spermatozoa motility and velocity at 30s postactivation in all groups. Over 48h of storage, the highest spermatozoa motility and velocity were obtained in sperm diluted in IM2 compared to the other groups. IM2 could maintain a significantly higher ATP content of diluted sperm than IM1 and undiluted treatment for 2days. Similarly, the highest values of eyeing and hatching rates were observed in sperm diluted in IM2 compared to sperm in the other studied groups. It can be concluded that the obtained result is a novel and applicable approach to maintain semen quality of pikeperch during short-term storage, suggesting IM2 as a promising medium for short-term storage. The present study also opens possibilities for ensuring a reliable source of semen as a convenient approach for increasing genetic diversity in hatcheries.


Assuntos
Preservação do Sêmen , Sêmen , Animais , Feminino , Masculino , Análise do Sêmen/veterinária , Preservação do Sêmen/veterinária , Motilidade Espermática , Espermatozoides
11.
J Anim Sci ; 99(2)2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33453111

RESUMO

Improvements in bull reproductive performance are necessary to optimize the efficiency of cattle production. Female fertility has been enhanced through assisted reproductive technologies as well as genetic selection; however, improving beef bull fertility has been largely ignored. Phenotypes routinely collected at bull semen collection facilities are believed to affect fertility and provide the phenotypes necessary for a genetic evaluation. The first objective of this study was to determine the significant fixed effects for modeling beef bull fertility using data from bull semen collection facilities. The second objective was to estimate variance components, heritabilities, repeatabilities, and correlations between beef bull semen attributes. Beef bull fertility phenotypes including volume (VOL), concentration (CONC), number of spermatozoa (NSP), initial motility (IMot), post-thaw motility (PTMot), 3-h post-thaw motility (3HRPTMot), percentage of normal spermatozoa (%NORM), primary abnormalities (PRIM), and secondary abnormalities (SEC) were obtained from two bull semen collection facilities. A total of 1,819 Angus bulls with 50,624 collection records were analyzed. Of the fixed class and covariate effects tested, the significant class effects were collection location and collection day within year and the significant covariate effects included age at collection, days since previous collection, and cumulative comprehensive climate index (CCI). For this study, the CCI was calculated for a 75-d period including the 61-d spermatogenesis cycle and 14-d epididymal transit time. The 75 d prior to collection accounted for the environmental stress a bull may have experienced over the course of development of the spermatozoa, which was more significant than the CCI calculated for collection day or spermatogenesis start date. Pre-thaw beef bull semen traits had low heritability estimates of 0.11 ± 0.02 (VOL), 0.09 ± 0.02 (CONC), 0.08 ± 0.02 (NSP), and 0.12 ± 0.03 (IMot). Heritabilities of post-thaw beef bull semen attributes were more variable at 0.10 ± 0.02 (PTMot), 0.05 ± 0.04 (3HRPTMot), 0.10 ± 0.04 (%NORM), 0.03 ± 0.03 (PRIM), and 0.18 ± 0.04 (SEC). Correlations of breeding values for these traits with scrotal circumference (SC) expected progeny difference (EPD) are low. The low to moderate heritability estimates indicate that genetic improvement can be made in beef bull semen quality traits if new tools are developed to augment the scrotal circumference EPD that are currently available within the industry.


Assuntos
Fertilidade/genética , Análise do Sêmen , Sêmen , Animais , Bovinos/genética , Feminino , Masculino , Análise do Sêmen/veterinária , Espermatogênese , Espermatozoides
12.
Ecotoxicol Environ Saf ; 208: 111694, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33396025

RESUMO

Experimental studies have shown that nonradioactive strontium (Sr), in the form of Sr2+, have a positive effect on semen quality, but human evidence is lacking. This study aimed to examine the associations between nonradioactive Sr exposure and semen quality in Chinese men (n = 394). We recruited men who presented at an infertility clinic in Wuhan, China to seek for semen parameter analyses. Urinary Sr concentration as an exposure biomarker was measured using inductively coupled plasma mass spectrometer. We estimated the associations between urinary Sr concentrations and semen parameters using multivariable logistic and linear regression models. In multivariable linear regressions models, positive dose-response associations were estimated for sperm concentration, motility, and count across increasing urinary Sr quartiles (all p for trends<0.05), and the consistent positive associations were also observed for urinary Sr concentration modeled as a continuous exposure. In multivariable logistic models, decreased risks of below-reference sperm concentration, motility, and count were also estimated across increasing urinary Sr quartiles (all p for trends<0.05). Our results suggest that nonradioactive Sr exposure may have a beneficial effect on semen quality, but more investigations are warranted to confirm the results.


Assuntos
Exposição Ambiental/análise , Análise do Sêmen , Estrôncio/urina , Adulto , Biomarcadores/urina , China , Clínicas de Fertilização , Humanos , Masculino , Contagem de Espermatozoides , Motilidade Espermática , Espermatozoides/citologia
13.
Trop Anim Health Prod ; 53(1): 103, 2021 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-33417110

RESUMO

To investigate the effect of supplementation of L-arginine (AR) on sub-fertile buffalo-bulls' ejaculates, 25 ejaculates of poor motility (40 to 55%) were collected by artificial vagina from 5 buffalo-bulls and extended with Tris-yolk extender (1:10) supplemented with different concentrations of AR (0, 3, 4, 5, and 6 mM). Semen was cooled gradually to 4 °C within 2 h and incubated at 4 °C for additional 2 h. Incubated semen samples were evaluated by computer-assisted semen analysis. Results showed that addition of 5 mM AR increased (P < 0.05) total sperm motility and rapid progressive motility percentages, while decreased (P < 0.05) non-motile sperm and static sperm percentages compared with AR-free (control) extender. Increasing the AR level to 6 mM increased (P < 0.05) the percentages of sperm progressive motility and rapid and slow progressive motilities, while decreased (P < 0.05) the non-progressive sperm motility percentages compared with AR-free extender. Supplementation of 5 mM AR improved (P < 0.05) sperm straight linear, curve linear, and average path velocities (36 ± 0.13, 20.6 ± 5.3, and 33.2 ± 8.5, respectively) in comparing with control and other AR treatments. Addition of AR (5 and 6 mM) improved (P < 0.05) the percentages of vitality (89.8 ± 1.9 and 80.0 ± 3.4, respectively), normality (44.3 ± 3.6 and 44.8 ± 1.5, respectively), and functional sperm (20.4 ± 8.6 and 21.0 ± 0.61, respectively), and decreased abnormal neck and tail percentages compared with AR-free extender. All AR levels decreased (P < 0.05) the abnormal neck and tail percentages. Addition of all AR levels had no significant (P > 0.05) effect on the activity of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in semen extender. Supplementation of Tris-yolk extender with L-arginine (5 or 6 mM) can improve sperm motility, velocity, vitality, and functional sperm and can decrease tail and neck abnormalities of sub-fertile buffalo ejaculate after 4 h incubation at cool temperature.


Assuntos
Arginina/farmacologia , Búfalos/fisiologia , Crioprotetores/farmacologia , Ração Animal/análise , Animais , Arginina/administração & dosagem , Arginina/metabolismo , Criopreservação/veterinária , Crioprotetores/administração & dosagem , Crioprotetores/metabolismo , Dieta/veterinária , Suplementos Nutricionais/análise , Relação Dose-Resposta a Droga , Masculino , Sêmen/efeitos dos fármacos , Análise do Sêmen/veterinária , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/efeitos dos fármacos
14.
Lancet ; 397(10271): 319-333, 2021 01 23.
Artigo em Inglês | MEDLINE | ID: mdl-33308486

RESUMO

It is estimated that infertility affects 8-12% of couples globally, with a male factor being a primary or contributing cause in approximately 50% of couples. Causes of male subfertility vary highly, but can be related to congenital, acquired, or idiopathic factors that impair spermatogenesis. Many health conditions can affect male fertility, which underscores the need for a thorough evaluation of patients to identify treatable or reversible lifestyle factors or medical conditions. Although semen analysis remains the cornerstone for evaluating male infertility, advanced diagnostic tests to investigate sperm quality and function have been developed to improve diagnosis and management. The use of assisted reproductive techniques has also substantially improved the ability of couples with infertility to have biological children. This Seminar aims to provide a comprehensive overview of the assessment and management of men with infertility, along with current controversies and future endeavours.


Assuntos
Infertilidade Masculina/diagnóstico , Infertilidade Masculina/terapia , Espermatogênese/fisiologia , Feminino , Humanos , Masculino , Gravidez , Taxa de Gravidez/tendências , Técnicas de Reprodução Assistida/estatística & dados numéricos , Análise do Sêmen/normas
16.
J Urol ; 205(1): 36-43, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33295257

RESUMO

PURPOSE: The summary presented herein represents Part I of the two-part series dedicated to the Diagnosis and Treatment of Infertility in Men: AUA/ASRM Guideline. Part I outlines the appropriate evaluation of the male in an infertile couple. Recommendations proceed from obtaining an appropriate history and physical exam (Appendix I), as well as diagnostic testing, where indicated. MATERIALS/METHODS: The Emergency Care Research Institute Evidence-based Practice Center team searched PubMed®, Embase®, and Medline from January, 2000 through May, 2019. When sufficient evidence existed, the body of evidence was assigned a strength rating of A (high), B (moderate), or C (low) for support of Strong, Moderate, or Conditional Recommendations. In the absence of sufficient evidence, additional information is provided as Clinical Principles and Expert Opinions (table 1[Table: see text]). This summary is being simultaneously published in Fertility and Sterility and The Journal of Urology. RESULTS: This Guideline provides updated, evidence-based recommendations regarding evaluation of male infertility as well as the association of male infertility with other important health conditions. The detection of male infertility increases the risk of subsequent development of health problems for men. In addition, specific medical conditions are associated with some causes for male infertility. Evaluation and treatment recommendations are summarized in the associated algorithm (figure[Figure: see text]). CONCLUSION: The presence of male infertility is crucial to the health of patients and its effects must be considered for the welfare of society. This document will undergo updating as the knowledge regarding current treatments and future treatment options continues to expand.


Assuntos
Infertilidade Masculina/diagnóstico , Medicina Reprodutiva/normas , Urologia/normas , Aconselhamento/normas , Medicina Baseada em Evidências/métodos , Medicina Baseada em Evidências/normas , Humanos , Infertilidade Masculina/etiologia , Infertilidade Masculina/terapia , Estilo de Vida , Masculino , Medicina Reprodutiva/métodos , Escroto/diagnóstico por imagem , Análise do Sêmen , Sociedades Médicas/normas , Ultrassonografia , Estados Unidos , Urologia/métodos
17.
J Urol ; 205(1): 44-51, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33295258

RESUMO

PURPOSE: The summary presented herein represents Part II of the two-part series dedicated to the Diagnosis and Treatment of Infertility in Men: AUA/ASRM Guideline. Part II outlines the appropriate management of the male in an infertile couple. Medical therapies, surgical techniques, as well as use of intrauterine insemination (IUI)/in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) are covered to allow for optimal patient management. Please refer to Part I for discussion on evaluation of the infertile male and discussion of relevant health conditions that are associated with male infertility. MATERIALS/METHODS: The Emergency Care Research Institute Evidence-based Practice Center team searched PubMed®, Embase®, and Medline from January 2000 through May 2019. When sufficient evidence existed, the body of evidence was assigned a strength rating of A (high), B (moderate), or C (low) for support of Strong, Moderate, or Conditional Recommendations. In the absence of sufficient evidence, additional information is provided as Clinical Principles and Expert Opinions (table[Table: see text]). This summary is being simultaneously published in Fertility and Sterility and The Journal of Urology. RESULTS: This Guideline provides updated, evidence-based recommendations regarding management of male infertility. Such recommendations are summarized in the associated algorithm (figure[Figure: see text]). CONCLUSION: Male contributions to infertility are prevalent, and specific treatment as well as assisted reproductive techniques are effective at managing male infertility. This document will undergo additional literature reviews and updating as the knowledge regarding current treatments and future treatment options continues to expand.


Assuntos
Infertilidade Masculina/terapia , Medicina Reprodutiva/normas , Urologia/normas , Varicocele/terapia , Aconselhamento/normas , Suplementos Nutricionais , Medicina Baseada em Evidências/métodos , Medicina Baseada em Evidências/normas , Fertilização In Vitro/métodos , Fertilização In Vitro/normas , Humanos , Infertilidade Masculina/diagnóstico , Infertilidade Masculina/etiologia , Masculino , Medicina Reprodutiva/métodos , Escroto/diagnóstico por imagem , Moduladores Seletivos de Receptor Estrogênico/uso terapêutico , Análise do Sêmen , Sociedades Médicas/normas , Recuperação Espermática/normas , Resultado do Tratamento , Estados Unidos , Urologia/métodos , Varicocele/complicações , Varicocele/diagnóstico
18.
Ecotoxicol Environ Saf ; 207: 111563, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-33254417

RESUMO

Exposure to endocrine-disrupting chemicals (EDCs) has been hypothesized as a cause of declining sheep reproductive efficiency. Understanding the long-term effects of EDCs such as heavy metals on reproductive health requires investigation in 'real life' of sheep that are reared in industrial areas. The aim of this study was to evaluate the effect of long-term exposure of Kermani rams to high levels of environmental heavy metals probably emitted from a copper smelter at KhatoonAbad in ShahreBabak, Kerman province. Testicular characteristics were determined in randomly-selected rams (3-4 years old) at 4 directions (south, north, east, and west) and 4 distances (10, 20, 30, and 40 km) from the smelter. Testicular trace element contents, size, serum testosterone, histological attributes and seminal characteristics, except semen volume, were affected by both the direction and the distance from the smelter (P < 0.05). Testicular contents of Pb, Cd, Cr, and Ni, and sperm abnormalities were higher at 10 km south from the smelter and lower at 40 km west. Other parameters were higher at 40 km west and lower at 10 km south. Interestingly, the testicular contents of Cu at 10 km south were lower and associated with higher sperm abnormalities in the rams reared closer to the smelter. The highest weight, length and circumference of the testis were found at 40 km west. The lowest concentration of testosterone was observed at 10 km south, being 92.6% lower than the highest values obtained at 40 km west. The diameter of seminiferous tubules and epithelial height at 10 km south were 8.9% and 27.5% lower than the highest values obtained at 40 km west. A positive correlation between Pb, Cd, Cr and Ni contents in the testis with sperm abnormalities, and a negative correlation between these elements with the other parameters were found. It was concluded that long-term exposure to heavy metals might have been a cause of decreased fertility in rams and probably other living species in this region.


Assuntos
Poluentes Ambientais/toxicidade , Metais Pesados/toxicidade , Testículo/efeitos dos fármacos , Animais , Cobre/toxicidade , Masculino , Reprodução , Análise do Sêmen/veterinária , Ovinos , Espermatozoides/efeitos dos fármacos , Espermatozoides/fisiologia , Testículo/fisiologia , Testosterona/sangue , Testes de Toxicidade Crônica , Oligoelementos/metabolismo
19.
Chemosphere ; 263: 128178, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33297146

RESUMO

The reproductive toxicity of fluoride (F) has been verified by various epidemiological and experimental studies. Our previous work suggested that the interleukin 17A (IL-17A) is involved in the testicular damage induced by excessive F exposure. In this study, we further investigated the role of IL-17A in F-induced testicular injury. Wild type (WT) and IL-17A knockout (IL-17A-/-) mice were exposed to 0, 25, 50, or 100 mg/L sodium fluoride (NaF) for 90 days. We found that exposure to excessive F levels caused testicular damage, decreased semen quality, negatively affected testicular morphology, and increased the inflammatory response. Specifically, excessive F intake increased the expression levels of IL-17A in the testis and increased the protein levels of Act1, NF-κB, IL-17R, C/EBP-α, and TRAF6 in the IL-17A signaling pathway. The increase in IL-17A expression corresponded to increases expression of IL-17R, IL-6, IL-23, IL-1ß, TGF-ß and TNF-α as assessed by RT-PCR and ELISA assays. Remarkably, IL-17A knockout in mice ameliorated the effects of F on testicular damage, semen quality, testicular morphology, and the immune response. Additionally, we found the in vitro exposure of Leydig cells to NaF and recombinant IL-17A led to abnormal apoptosis and a decrease in testosterone secretion. Our findings prove that IL-17A plays a key role in the exacerbation of testicular injuries in F-exposed mice, and that IL-17A deficiency can alleviate F-induced injury by inhibiting the immune response and apoptosis in the testis. These data suggest that targeting IL-17A may be a useful therapeutic strategy for treating F-mediated toxicity in the testis.


Assuntos
Fluoretos , Interleucina-17 , Animais , Apoptose , Humanos , Imunidade , Interleucina-17/genética , Masculino , Camundongos , Análise do Sêmen
20.
J Urol ; 205(1): 236-240, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-32716681

RESUMO

PURPOSE: We retrospectively evaluated the utility of sperm cryopreservation at the time of vasectomy reversal. MATERIALS AND METHODS: From April 2016 through December 2018 a total of 26 men underwent vasectomy reversal. Sperm cryopreservation is routinely offered at the time of vasectomy reversal at our institution. We sought to assess utilization of cryopreserved sperm by those men with early or late vasectomy reversal failure. RESULTS: Of 26 patients presenting for vasectomy reversal 22 (85%) elected to cryopreserve sperm. Sperm were obtained for freezing from the vasal (7 patients) or epididymal fluid (3), or via testicular biopsy (12). Three patients were lost to followup postoperatively. Of the 23 who presented for post-procedure followup 19 either had semen analyses with motile sperm or a live birth (83% success rate). There were 4 early failures and 2 late failures; all patients with failures had elected to cryopreserve sperm at the time of initial reversal. Three of the 6 individuals (50%) with vasectomy reversal failure elected to use cryopreserved sperm for in vitro fertilization/intracytoplasmic sperm injection, with 2 of these (67%) resulting in live births. CONCLUSIONS: Of those patients who experienced vasectomy reversal failure 50% elected to use cryopreserved sperm that had been procured at the time of initial reversal. Given the potential for early or late failure, cryopreservation of sperm at the time of vasectomy reversal should be routinely offered as a means of avoiding the added expense and potential morbidity of future surgical sperm retrieval.


Assuntos
Criopreservação , Recuperação Espermática , Espermatozoides , Vasectomia/efeitos adversos , Vasovasostomia/efeitos adversos , Adulto , Feminino , Humanos , Nascimento Vivo , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Análise do Sêmen , Injeções de Esperma Intracitoplásmicas/estatística & dados numéricos , Falha de Tratamento , Vasovasostomia/métodos
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