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2.
Arerugi ; 69(1): 40-47, 2020.
Artigo em Japonês | MEDLINE | ID: mdl-32051368

RESUMO

BACKGROUND: Widely accepted loading protocols for rush subcutaneous immunotherapy (rSCIT) have not been established. Our aim was to evaluate the loading protocols of rSCIT. METHODS: In the low initial dose group (33 patients), the initial dose of standardized house dust mite extract was 1 JAU or less. The target dose at the end of the rush build-up phase was 500 JAU. Next, the initial dose was increased to 10 JAU with the same target dose in the high initial dose group (18 patients). Furthermore, in the modified high initial dosage group (17 patients), the initial dose was 10 JAU but the target dose at the end of the rush phase was 300 JAU. Then, the maintenance dose of 500 JAU was administered at 9 or 10 days after rSCIT initiation. We retrospectively evaluated these protocols. RESULTS: A systemic reaction (SR) occurred in 28 out of 33 (84.8%) patients in the low initial dosage group and in 12 out of 18 (66.7%) patients in the high initial dosage group, on the other hand significantly reduced in 4 out of 17 (23.5%) patients in the modified high-dosage group. The amount of antigen reached 339.3±19.0 JAU in the low initial dosage group and 358.3±24.9 JAU in the high initial dosage group at the end of the rush phase, significantly increased 452.9±20.6 JAU in the modified high-dosage group at 9 or 10 days. CONCLUSION: In rSCIT using standardized house dust mite extract, lowering the target dose at the end of the rush phase and delaying the administration of the maintenance dose may reduce SR and increase the reached amount of antigen.


Assuntos
Alérgenos , Antígenos de Dermatophagoides/administração & dosagem , Dessensibilização Imunológica , Pyroglyphidae , Animais , Extratos Celulares/administração & dosagem , Humanos , Estudos Retrospectivos
3.
Artigo em Chinês | MEDLINE | ID: mdl-32086918

RESUMO

Objective:The aim of this study is to investigate the effect of sublingual immunotherapy with Dermatophagoides Farinae on the expression of specific IgG4(sIgG4) in patients with allergic rhinitis (AR) in Hainan area. Method:Seventy-two patients with dust-mite allergic rhinitis, all three generations of whom were local islanders in Hainan, were randomly divided into control group(36 cases) and SLIT group(36 cases). sIgG4 and sIgE expression levels were detected before treatment, 6 months after treatment, 12 months after treatment, and 18 months after treatment. The patient's symptom score, medication score, VAS score and adverse reactions was also assessed. Finally, through statistical analysis of the relevant data collected at 4 time points in the two groups of patients, the efficacy, safety and changes of sIgG4 antibody expression level in patients with allergic rhinitis receiving sublingual specific immunotherapy in Hainan were observed. Result:Symptoms scores, medication scores and VAS scores were significantly improved in the SLIT group after treatment compared with before treatment(P<0.05), and serum sIgG4 increased significantly(P<0.01), serum sIgE showed no significant change(P>0.05). In the control group, symptom scores, medication scores and VAS scores were also significantly improved compared with before treatment(P<0.05), while serum sIgG4 and sIgE showed no significant change(P>0.05). When comparing the two groups, Symptoms scores, medication scores and VAS scores of the SLIT group were significantly lower than those of the control group at 12 months and 18 months after treatment(P<0.05). sIgG4 expression levels in the SLIT group were significantly higher than those in the control group after 6, 12 and 18 months of treatment(P<0.01). There was no significant difference in sIgE expression level between the two groups(P>0.05). No severe systemic adverse reactions occurred in the two groups, and 3 patients showed mild adverse reactions in the SLIT group. Conclusion:Sublingual immunotherapy of Dermatophagoides Farinae was effective and could increase the expression of sIgG4 in patients with Dermatophagoides farinae AR, sIgG4 is expected to be an immunological marker for the objective evaluation of the clinical efficacy of Hapten.


Assuntos
Antígenos de Dermatophagoides/uso terapêutico , Imunoglobulina G/sangue , Rinite Alérgica/terapia , Imunoterapia Sublingual , Animais , China , Dermatophagoides farinae , Humanos , Rinite Alérgica/imunologia , Resultado do Tratamento
4.
Artigo em Chinês | MEDLINE | ID: mdl-32086899

RESUMO

Objective:Study the efficacy of sublingual immunotherapy (SLIT) for monosensitized and polysensitized allergic rhinitis patients of different severity. Method:According to the results of intradermal experiments, 186 patients with AR who were treated with standardized dust mites SLIT for 2 years. These patients have been divided into mild monosensitized group and mild polysensitized group, moderate monosensitized group and moderate polysensitized group, severe monosensitized group and severe polysensitized group. Statistics and analysis of total nasal symptom score (TNSS), total medication score (TMS) , visual analog scale (VAS) about before and after SLIT treatment. Result:Before SLIT treatment and after 2 years, the TNSS: Mild monosensitized group (5. 58±1. 68)(0. 58±0. 75), mild polysensitized group(5. 34±1. 49)(0. 93±0. 75);moderate monosensitized group (4. 68±1. 55)(0. 32±0. 63), moderate polysensitized group(6. 26±1. 46)(0. 61±0. 67),which have showed a statistically significant difference(P<0.05),between the two groups have no significant difference (P>0.05). Severe monosensitized group(6. 26±0. 73)(1. 16±1. 13), severe polysensitized group(6. 49±1. 04)(1. 89±1. 07), which have showed a statistically significant difference(P<0.05), between the two groups have a statistically significant difference(P<0.05). The VAS:Mild monosensitized group (6. 09±1. 23)(1. 52±1. 25), mild polysensitized group(6. 21±0. 90)(1. 97±1. 18),moderate monosensitized group(6. 12±0. 53)(1. 16±1. 03), moderate polysensitized group(6. 65±1. 08)(1. 65±0. 98),which have showed a statistically significant difference(P<0.05),between the two groups have no significant difference (P>0.05). Severe monosensitized group(6. 26±0. 73)(1. 16±1. 13), severe polysensitized group(6. 49±1. 04)(1. 89±1. 07), which have showed a statistically significant difference(P<0.05), between the two groups have a statistically significant difference(P<0.05). The TMS:These have showed a statistically significant difference(P<0.05)with same group,between all groups have no significant difference (P>0.05). Conclusion:Dust mites under the SLIT are effective for monosensitized and polysensitized allergic patients. For patients with severe polysensitized , it is necessary to extend the treatment time (>2 years).


Assuntos
Rinite Alérgica/terapia , Imunoterapia Sublingual , Antígenos de Dermatophagoides/uso terapêutico , Humanos , Rinite Alérgica/classificação , Resultado do Tratamento
5.
Int Arch Allergy Immunol ; 181(1): 71-80, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31722337

RESUMO

BACKGROUND: Few studies have directly compared the immunologic responses to specific subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT). OBJECTIVE: We aimed to directly compare clinical efficacy and immunological responses between SLIT and SCIT in allergic rhinitis (AR) sensitized to house dust mites. METHODS: Sixty-seven patients (age 5-55 years) with moderate-severe Dermatophagoides pteronyssinus (Der-p) and Dermatophagoides farinae AR with or without asthma were randomized (2:2:1) into SLIT (n = 27), SCIT (n = 26) and placebo (n = 14) groups. Symptom and medication scores, visual analogue score, serum Der-p specific immunoglobulin G4 (Der-p-sIgG4), CD4+CD25+FoxP3+ regulatory T cells (Tregs) and serum cytokines were measured. RESULTS: After 1-year treatment, a significant improvement of total rhinitis score (TRS), total rhinitis medication score (TRMS) and visual analogue score occurred in both SLIT and SCIT. There were no differences in clinical efficacy except for TRMS (p = 0.026) when SLIT and SCIT were directly compared. CD4+CD25+FoxP3+ Tregs had a trend towards upregulation in the 2 modes and inversely correlated with TRS (p = 0.024) only in SLIT. Der-p-sIgG4 significantly increased in SLIT and SCIT (p < 0.05), and it was 30 times higher in SCIT than SLIT after the treatment (p < 0.05). Serum interferon-γ significantly increased only in SCIT after 1 (p = 0.008), 6 (p = 0.007) and 12 (p = 0.008) months of treatment and inversely correlated with TRS (p = 0.032). CONCLUSION: While SCIT and SLIT have similar rates of clinical improvement, the 2 modes reveal heterogeneous changes of CD4+CD25+Foxp3+ Tregs, sIgG4 and cytokines.


Assuntos
Asma/terapia , Citocinas/sangue , Rinite Alérgica/terapia , Imunoterapia Sublingual/métodos , Linfócitos T Reguladores/imunologia , Adolescente , Adulto , Antígenos de Dermatophagoides/imunologia , Asma/complicações , Asma/imunologia , Antígenos CD4/metabolismo , Criança , Pré-Escolar , Estudos Controlados Antes e Depois , Feminino , Fatores de Transcrição Forkhead/metabolismo , Humanos , Infusões Subcutâneas , Subunidade alfa de Receptor de Interleucina-2/metabolismo , Masculino , Pessoa de Meia-Idade , Rinite Alérgica/complicações , Rinite Alérgica/imunologia , Adulto Jovem
6.
Ther Umsch ; 76(6): 293-299, 2019 Nov.
Artigo em Alemão | MEDLINE | ID: mdl-31762417

RESUMO

Allergy assessment: when and how? Abstract. Allergies are common in the clinical practice. The most important allergens are pollen, house dust mites, animal dander, mold and allergens attributable to a particular work environment. The medical history is a very important part for diagnosing an allergy, because sensitizations detected by skin prick tests and laboratory tests are common but not always symptomatic and therefore without clinical relevance. There are 3 options to manage allergic diseases: avoidance of allergens, symptomatic treatment and - in selected cases - a causal treatment like allergen-specific immunotherapy.


Assuntos
Alérgenos/imunologia , Hipersensibilidade , Antígenos de Dermatophagoides , Reações Cruzadas , Humanos , Hipersensibilidade/diagnóstico , Testes Cutâneos/métodos
7.
Int J Mol Sci ; 20(19)2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31581442

RESUMO

Garlic (Allium sativum L.) has been used extensively as a food ingredient and medicinally, but the effect on asthmatic airway inflammation has not been studied in detail. We accordingly explored the protective effects exerted by various garlic fraction extracts against airway inflammation with Dermatophagoides pteronyssinus (Der p)-induced allergic asthma in vivo and in vitro. Garlic extraction was realized using n-hexane, dichloromethane, ethylacetate, n-butanol, and water in sequence to obtain different fraction extracts. Mice were orally administered different fractions (80 mg/kg) daily for four weeks. The histological results showed that the water fraction could ameliorate lung-based goblet cell hyperplasia, inflammatory cell infiltration, and mucus hypersecretion. The water fraction extracts decreased IgE and IgG1, and they decreased inflammatory cells as quantified in bronchoalveolar lavage fluid (BALF); however, they increased IgG2a in serum. Moreover, the water fraction extracts increased IFN-γ and IL-12 (both constituting Th1 cytokines) in BALF, but they reduced IL-13, -4, and -5 (all constituting Th2 cytokines), and also inhibited the expression of IL-1ß, IL-6, and TNF-α. The water fraction also inhibited the PI3K/Akt/NF-κB signal pathways in A549 cells. These findings suggest that water fraction extracts of garlic have a clear anti-inflammatory effect on Der p-induced allergic asthma.


Assuntos
Antiasmáticos/farmacologia , Antígenos de Dermatophagoides/imunologia , Alho/química , Extratos Vegetais/farmacologia , Substâncias Protetoras/farmacologia , Hipersensibilidade Respiratória/imunologia , Animais , Antiasmáticos/química , Antiasmáticos/isolamento & purificação , Líquido da Lavagem Broncoalveolar , Cromatografia Líquida de Alta Pressão , Citocinas/metabolismo , Modelos Animais de Doenças , Imunoglobulina E/imunologia , Imunoglobulina G/imunologia , Mediadores da Inflamação/metabolismo , Contagem de Leucócitos , Camundongos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Substâncias Protetoras/química , Substâncias Protetoras/isolamento & purificação , Hipersensibilidade Respiratória/diagnóstico , Hipersensibilidade Respiratória/tratamento farmacológico , Hipersensibilidade Respiratória/metabolismo , Transdução de Sinais
8.
Allergol. immunopatol ; 47(5): 467-476, sept.-oct. 2019. tab, graf
Artigo em Inglês | IBECS | ID: ibc-186521

RESUMO

Background: House dust mite (Dermataphagoides pteronyssinus) is a widespread risk factor in the development of asthma. CD4+ T lymphocytes have an important role in the pathogenesis of allergic asthma by polarizing to Th2 cells. Objective: We aimed to evaluate the immunoregulatory effects of dental follicle mesenchymal stem cells with and without IFN-γ stimulation on peripheral blood mononuclear cells of house dust mite sensitive asthmatic patients, and compared those with Dexamethasone as a systemic steroid. Material and methods: PBMC of asthmatic patients and healthy individuals separately cultured with or without DF-MSCs in the presence and absence of IFN-γ or Der p1 or Dexamethasone for 72h. CD4+ T proliferation, cell viability, CD4+CD25+FoxP3+ Treg cell frequency and cytokine profiles of PBMC were evaluated via flow cytometry. Results: DF-MSCs suppressed proliferation of CD4+ T lymphocytes (pCDmix < 0.01, pDerp1 < 0.01, pIFN < 0.005) by increasing the number of FoxP3 expressing CD4 + CD25 + T regulatory cells (pCDmix < 0.005, pDerp1 < 0.01, pIFN < 0.001) and suppressed lymphocyte apoptosis (pCDmix < 0.05, pDerp1< 0.05, pIFN < 0.05), while Dexamethasone increased the apoptosis and decreased Treg cell frequency in asthmatic patients. IFN-γ stimulation increased the suppressive effect of DF-MSCs and also enhanced the frequency of FoxP3 expressing CD4+CD25 + T regulatory cells. The cytokine levels were regulated by DF-MSCs by reducing IL-4 cytokine levels (pCDmix < 0.01, pDerp1 < 0.05, pIFN < 0.05) and upregulating IFN-γ levels (pCDmix < 0.01, pDerp1< 0.05, pIFN < 0.005) in asthmatic patients. Conclusion: IFN-γ stimulated DF-MSCs were found to have a high modulatory effect on CD4 + T cell responses, while Dexamethasone had an apoptotic effect on CD4+ T cells in asthmatic patients. DF-MSCs may be a new cell-based therapy option for allergic diseases including asthma


No disponible


Assuntos
Humanos , Animais , Masculino , Feminino , Adulto Jovem , Adulto , Asma/imunologia , Linfócitos T CD4-Positivos/imunologia , Saco Dentário/patologia , Dermatophagoides pteronyssinus/imunologia , Interferon gama/imunologia , Células-Tronco Mesenquimais/imunologia , Antígenos de Dermatophagoides/imunologia , Proteínas de Artrópodes/imunologia , Células Cultivadas , Imunidade Celular , Imunização
9.
Allergol. immunopatol ; 47(5): 417-424, sept.-oct. 2019. tab
Artigo em Inglês | IBECS | ID: ibc-186515

RESUMO

Introduction: The association regarding the atopic sensitization to mite aeroallergens and the socio-environmental features is still inconsistent. Objectives: We analyzed the role played by socioeconomic and environmental factors in the prevalence of sensitization to house dust mite (HDM) allergens, and associated with the risk of developing asthma symptoms. Patients and methods: This is a case-control study conducted with 108 patients, aged 1-17. We inquired about family habits, socioeconomic and environmental features. We applied the International Study of Asthma and Allergies in Childhood questionnaire. Results: We observed patients sensitized to all HDM tested, Derp (42%), Derf (37%) and Blot (33%). Middle family income (OR: 2.74; CI95%: 1.127-6.684), exposure to dog (OR: 3.758, CI95%: 1.127-6.684) and artificial climatization (OR: 4.319, CI95%: 1.398-13.348) were associated with sensitization to Derp. We also observed protective factors, such as sharing of dormitories, washing cycle for bedspreads and the presence of basic sanitation. An increased risk of sensitization to Derf was associated with Blot sensitization (OR: 3.172, CI95%: 1.083-9.292) and presence of mold on the walls (OR: 3.095, CI95%: 1.063-9.008). A protective factor was dormitory sharing. For sensitization to Blot, we observed an increase in the risk associated with Derp sensitization (OR: 3.462, CI95%: 1.191-10.061) and exposure to dog (OR: 3.255, CI95%: 0.987-10.736). In addition, sensitization to Blot increases the risk of developing asthma symptoms (OR: 2.732, CI95%: 0.981-7.606). Conclusion: Our data show distinct sociodemographic and environmental relations that lead to HDM sensitization and increased probability of development of allergic diseases


No disponible


Assuntos
Humanos , Animais , Masculino , Feminino , Lactente , Pré-Escolar , Adolescente , Asma/epidemiologia , Exposição Ambiental/efeitos adversos , Hipersensibilidade/epidemiologia , Fatores Socioeconômicos , Antígenos de Dermatophagoides/imunologia , Brasil/epidemiologia , Estudos de Casos e Controles , Prevalência , Pyroglyphidae/imunologia
10.
Exp Parasitol ; 206: 107754, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31473211

RESUMO

Dermatophagoides farinae is an important source of indoor allergens that shows strong tolerance to external temperatures. However, the regularity and mechanism of tolerance are still unclear. Based on our previous RNA-seq and annotation of D. farinae under temperature stress, it is planned to identify differentially expressed genes (DEGs) involved in the temperature stress response by quantitative real-time PCR (qRT-PCR). However, the lack of reference genes directly limited the detection and confirmation of DEGs. Accordingly, in this study, we have selected six candidates as reference genes in D. farinae: 60S RP L11, 60S RP L21, α tubulin, GAPDH, Der f Mal f 6, and calreticulin, and evaluated their expression stabilities as affected by heat and cold stresses, using geNorm, NormFinder, BestKeeper, comparative ΔCt and RefFinder methods. Then the expression level of 15 DEGs were detected and verified. geNorm analysis showed that α tubulin and calreticulin were the most stable reference genes under heat stress and cold stress of D. farinae. Similar evaluation results were obtained by NormFinder and BestKeeper, in which 60S RP L21 and α tubulin were the most stable reference genes. By comparative ΔCt method and a comprehensive evaluation of RefFinder, α tubulin was identified as the most ideal reference gene of D. farinae under heat and cold stresses. Furthermore, qRT-PCR detection results of 15 DEGs were almost identical to the RNA-seq results, indicating that α tubulin is stable as a reference gene. This study provided technical support for DEGs expression studies in D. farinae using qRT-PCR.


Assuntos
Calreticulina/genética , Dermatophagoides farinae/genética , Temperatura , Tubulina (Proteína)/genética , Animais , Antígenos de Dermatophagoides/genética , Primers do DNA/química , Dermatophagoides farinae/fisiologia , Feminino , Amplificação de Genes , Perfilação da Expressão Gênica , Gliceraldeído-3-Fosfato Desidrogenase (Fosforiladora)/genética , Anotação de Sequência Molecular , RNA/química , RNA/genética , RNA/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real , Proteínas Ribossômicas/genética , Análise de Sequência de RNA , Transcriptoma/genética , Temperatura de Transição , Sequenciamento Completo do Exoma
11.
Int Arch Allergy Immunol ; 180(3): 173-181, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31537004

RESUMO

Allergic diseases affect more than 25% of the global population. Der p 2 is the major allergen of the house dust mite (HDM) Dermatophagoides pteronyssinus. Allergen-specific immunotherapy is the only treatment to change the course of allergic diseases. In this study, two synthesized Der p 2 peptides coupled to cross-reacting material 197 (CRM197) showed reduced IgE reactivity and allergenic activity. CRM197-coupled Der p 2 peptides induced rDer p 2-specific IgG1 antibodies in mice, which could inhibit HDM-allergic patients' IgE binding to rDer p 2. The immunity effects of CRM197-coupled Der p 2 peptides were studied in an rDer p 2-induced asthma mouse model. CRM197-coupled Der p 2 peptides can suppress asthmatic airway inflammation in this model. Analysis of IL-4, IL-5, and IFN-γ levels in bronchoalveolar lavage fluid revealed that the suppression was associated with a shift from a Th2 to a Th1 response. Thus, CRM197-bound Der p 2 peptides exhibited less allergenic activity than the rDer p 2 allergen, which preserved immunogenicity and may be candidates for mite allergy vaccines.


Assuntos
Antígenos de Dermatophagoides/imunologia , Proteínas de Artrópodes/imunologia , Asma/terapia , Proteínas de Bactérias/imunologia , Inflamação/terapia , Pulmão/imunologia , Peptídeos/imunologia , Hipersensibilidade Respiratória/terapia , Animais , Antígenos de Dermatophagoides/química , Proteínas de Artrópodes/química , Asma/imunologia , Proteínas de Bactérias/química , Citocinas/metabolismo , Modelos Animais de Doenças , Feminino , Humanos , Imunoglobulina E/metabolismo , Imunoglobulina G/metabolismo , Inflamação/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Peptídeos/química , Hipersensibilidade Respiratória/imunologia , Equilíbrio Th1-Th2 , Vacinas/imunologia
12.
Scand J Immunol ; 90(5): e12820, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31486098

RESUMO

Allergens are the main trigger that enhances airway type 2 inflammation, and the epithelium is the first line of defense that reacts to its exposure. Therefore, epithelial-derived mediators, such as interleukin (IL)-25, IL-33, thymic stromal lymphopoietin (TSLP) and ezrin, may play a role as alarmins in IL-4/IL-13 signaling in allergic asthma (AA). We investigated the serum levels of IL-25, IL-33, TSLP, ezrin, IL-4 and IL-13, after bronchial challenge with Dermatophagoides pteronyssinus in patients with AA. We examined 18 subjects: nine steroid-free stable patients with AA sensitized to D. pteronyssinus and nine non-atopic healthy subjects (HS). Bronchial allergen challenge was performed using inhaled D. pteronyssinus allergen. IL-4, IL-13, IL-25, IL-33, TSLP and ezrin levels in serum were measured by ELISA at two time points - before and 24 hours after bronchial allergen challenge. The serum levels of IL-25, TSLP and ezrin did not differ between AA and HS groups at baseline. However, after allergen exposure, significant increases in serum levels of IL-25, TSLP and ezrin were observed only in patients with AA. The serum level of IL-33 at baseline was significantly higher in the AA group compared with HS, but the allergen challenge did not provoke an increase of this cytokine in any group. IL-4 and IL-13 levels were significantly higher at baseline in the AA group compared with HS and, after allergen exposure, were significantly increased in the AA group, with no effect on HS. Thus, the epithelial-derived mediators IL-25, TSLP and ezrin, via IL4/IL13 signaling, enhance type 2 inflammation after bronchial challenge with D. pteronyssinus in AA.


Assuntos
Antígenos de Dermatophagoides/imunologia , Asma/sangue , Asma/imunologia , Testes de Provocação Brônquica/métodos , Interleucina-13/sangue , Interleucina-4/sangue , Animais , Citocinas/sangue , Proteínas do Citoesqueleto/sangue , Dermatophagoides pteronyssinus/imunologia , Humanos , Interleucina-17/sangue , Interleucina-33/sangue , Transdução de Sinais/imunologia
14.
Int Immunopharmacol ; 75: 105830, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31437788

RESUMO

Umbelliferone (UMB) is a coumarin derivative present in roots and barks of plants, such as Angelica decursiva, Artemisia capillaris, and orange. UMB has been previously reported to exhibit anti-inflammatory, anti-diabetic, and anti-cancer effects. However, the effect of UMB on atopic dermatitis (AD) remains unknown. The purpose of this study was to investigate the anti-atopic effects of UMB on 2,4-dinitrochlorobenzene (DNCB)- and house dust mite extract (Dermatophagoides farinae extract, DFE)-treated mice with AD-like skin lesions and on tumor necrosis factor (TNF)-α/interferon (IFN)-γ-treated HaCaT cells. In DNCB/DFE-treated mice, oral administration of UMB (20 and 40 mg/kg) for 28 days led to a significant decrease in ear thickness, spleen size and weight, serum levels of immunoglobulin E (IgE), IgG1, IgG2a, TNF-α, and interleukin 4 (IL-4), and mast cell infiltration; it also led to the suppression of pro-inflammatory cytokines and chemokines. In addition, UMB reduced the secretion of pro-inflammatory cytokines and chemokines in TNF-α/IFN-γ-treated HaCaT cells via regulation of MAPK, IkB-α/NF-κB, and STAT1 signaling pathways. Taken together, these results indicate that UMB ameliorates AD-associated symptoms and inflammation via regulation of various signaling pathways, suggesting that UMB might be a potential therapeutic agent of AD.


Assuntos
Antialérgicos/uso terapêutico , Anti-Inflamatórios/uso terapêutico , Dermatite Atópica/tratamento farmacológico , Umbeliferonas/uso terapêutico , Animais , Antialérgicos/farmacologia , Anti-Inflamatórios/farmacologia , Antígenos de Dermatophagoides , Linhagem Celular , Citocinas/sangue , Citocinas/genética , Citocinas/imunologia , Dermatite Atópica/sangue , Dermatite Atópica/imunologia , Dermatite Atópica/patologia , Dinitroclorobenzeno , Humanos , Queratinócitos/efeitos dos fármacos , Masculino , Camundongos Endogâmicos BALB C , Pele/efeitos dos fármacos , Pele/patologia , Umbeliferonas/farmacologia
15.
Artigo em Chinês | MEDLINE | ID: mdl-31446703

RESUMO

Objective:To evaluate the efficacy, asthma control level and pulmonary function of sublingual immunotherapy on allergic asthma and rhinitis in adult patient. Method:This retrospective analysis of 182 cases of adult patient from 18-60 years with allergic asthma and rhinitis, according to the patients' wishes they were divided into drug group(66 patients) and SLIT group(116 patients). Drug group patients were treated with low to moderate doses of inhaled corticosteroids+long-acting ß2 receptor agonists and mometasone furoate nasal spray+desloratadine tablets as symptomatic treatment. SLIT group was treated by SLIT with Dermatophagoides farina drops on this basis. Before treatment and 1 year, 2 year and 3 years after treatment, the ACT, PEF%, DASS, NASS, TNSS and TMS were evaluated. Result:The ACT, PEF%, DASS, NASS, TNSS and TMS had continuously improved significantly 1 year, 2 year and 3 years after treatment in both groups comparing with baseline(P<0.05). Compared with 1 year after treatment, SLIT group had lower ACT, PEF%, DASS, NASS, TNSS and TMS in 2 years after treatment(all P<0.05), whereas drug group had lower TMS and DASS(all P<0.05). In 3 years of treatment, only SLIT group resulted in significant continuous improvement in DASS and TMS(all P<0.05). After 1 year, 2 and 3 years of treatment, SLIT group resulted in significantly better ACT, PEF%, DASS, NASS and TMS than drug group(all P<0.05). After 2 and 3 years of treatment, TNSS of SLIT group were significantly lower than in drug group(P<0.05). Conclusion:Sublingual immunotherapy for 3 yearswith Dermatophagoides farinae dropsin adult patient with allergic asthma and rhinitis was more effective than drug therapy alone, and asthma control and lung function improved significantly.


Assuntos
Asma/terapia , Rinite Alérgica/terapia , Imunoterapia Sublingual , Administração Sublingual , Adulto , Animais , Antígenos de Dermatophagoides/administração & dosagem , Dermatophagoides farinae , Humanos , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento , Adulto Jovem
16.
Inhal Toxicol ; 31(5): 192-202, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-31345048

RESUMO

Background: Increasing evidence from rodent studies indicates that inhaled multi-walled carbon nanotubes (MWCNTs) have harmful effects on the lungs. In this study, we examined the effects of inhalation exposure to MWCNTs on allergen-induced airway inflammation and fibrosis. We hypothesized that inhalation pre-exposure to MWCNTs would render mice susceptible to developing allergic lung disease induced by house dust mite (HDM) allergen. Methods: Male B6C3F1/N mice were exposed by whole-body inhalation for 6 h a day, 5 d a week, for 30 d to air control or 0.06, 0.2, and 0.6 mg/m3 of MWCNTs. The exposure atmospheres were agglomerates (1.4-1.8 µm) composed of MWCNTs (average diameter 16 nm; average length 2.4 µm; 0.52% Ni). Mice then received 25 µg of HDM extract by intranasal instillation 6 times over 3 weeks. Necropsy was performed at 3 and 30 d after the final HDM dose to collect serum, bronchoalveolar lavage fluid (BALF), and lung tissue for histopathology. Results: MWCNT exposure at the highest dose inhibited HDM-induced serum IgE levels, IL-13 protein levels in BALF, and airway mucus production. However, perivascular and peribronchiolar inflammatory lesions were observed in the lungs of mice at 3 d with MWCNT and HDM, but not MWCNT or HDM alone. Moreover, combined HDM and MWCNT exposure increased airway fibrosis in the lungs of mice. Conclusions: Inhalation pre-exposure to MWCNTs inhibited HDM-induced TH2 immune responses, yet this combined exposure resulted in vascular inflammation and airway fibrosis, indicating that MWCNT pre-exposure alters the immune response to allergens.


Assuntos
Antígenos de Dermatophagoides/imunologia , Hipersensibilidade/fisiopatologia , Exposição por Inalação/efeitos adversos , Pulmão/fisiologia , Nanotubos de Carbono/toxicidade , Animais , Líquido da Lavagem Broncoalveolar , Relação Dose-Resposta Imunológica , Fibrose , Imunoglobulina E/sangue , Interleucina-13/análise , Masculino , Camundongos , Células Th2/imunologia
17.
Int Arch Allergy Immunol ; 180(2): 144-149, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31256165

RESUMO

BACKGROUND: Several foreign studies have shown long-term efficacy of sublingual immunotherapy (SLIT), but data on the long-term efficacy of SLIT in China are still lacking. OBJECTIVE: We aimed to prospectively evaluate the long-term efficacy of a 2-year SLIT with Dermatophagoides farinae(D. farinae) drops in mono- and polysensitized children with allergic rhinitis (AR). METHODS: Eighty house dust mite (HDM)-sensitized children (aged 4-11 years) with AR were enrolled in this prospective study. There were 40 children in both the monosensitized (to HDM only) and polysensitized groups. Both groups were treated with standardized SLIT (D. farinae drops) for 2 years, combined with pharmacotherapy according to their individual requirements, and were followed up for 7 years. A combined symptom and medication score (CSMS) was assessed and compared between the 2 groups during and after SLIT. Safety was evaluated based on adverse events (AEs). RESULTS: There were 31 (77.5%) monosensitized and 29 (72.5%) polysensitized children who completed the study. After 2-year SLIT, the CSMS of 2 groups significantly decreased compared to baseline. The improvement persisted during the first 5 years at each visit, with a significant difference (all p < 0.01). In the monosensitized group, the CSMS significantly increased during the 6th and the 7th year compared to year 2 (both p < 0.05). Meanwhile, the polysensitized group showed a significant worsening of CSMS from the 5th to the 7th year (all p < 0.05). Furthermore, there was a statistical difference between the 2 groups in the 5th year of the study (p < 0.05). No severe AEs were reported. CONCLUSIONS: The study confirmed the long-term effects which lasted for 7 years after 2-year SLIT in mono- and polysensitized children. Compared with the polysensitized children, the monosensitized children noted a more sustained benefit.


Assuntos
Antígenos de Dermatophagoides/administração & dosagem , Antígenos de Dermatophagoides/imunologia , Dermatophagoides farinae/imunologia , Rinite Alérgica/terapia , Imunoterapia Sublingual/métodos , Administração Sublingual , Alérgenos/administração & dosagem , Alérgenos/imunologia , Animais , Criança , Pré-Escolar , China , Feminino , Humanos , Masculino , Estudos Prospectivos , Imunoterapia Sublingual/efeitos adversos , Resultado do Tratamento
18.
Artigo em Chinês | MEDLINE | ID: mdl-31163552

RESUMO

Objective: To evaluate the clinical efficacy and safety of intralymphatic immunotherapy with cervical lymph node injection for allergic rhinitis. Method: A retrospective analysis of 81 patients with allergic rhinitis(AR) who had received specific immunotherapy with cervical lymph node injection in 2016 in the first people's Hospital of Foshan was conducted. The neck lymph node immunotherapy under the guidance of color Doppler ultrasound consisted of three sessions, and in each session 50 Tu(Arog) was delivered. The scores of nasal and ocular symptoms, drug score and adverse reactions during treatment were recorded before and after treatment, and the efficacy and safety were observed. Result: Before treatment, the nasal symptoms score of 81 AR cases of mite allergy was 7.00±1.65. After 1,2 and 3 sessions of cervical lymph node immune therapy and 1 year after completion of treatment, the nasal symptom scores were 4.37±1.88, 4.26±1.80, 4.22±1.80, and 4.09±2.10,respectively, which were significantly lower than that before treatment(P<0.01). The quality of life score was 53.68±9.28 before treatment, which decreased to 23.01±13.28 one year after treatment, and the difference was statistically significant(P<0.01). The drug score was 3.27±1.17 before treatment, which decreased to 1.00±1.05 1 years after treatment, and the difference was statistically significant(P<0.01). During treatment and 1-year follow-up, only 8 cases had mild local reactions, and no systemic adverse reactions occurred. Conclusion: Cervical lymph node injection specific immunotherapy can significantly relieve the symptoms of dust mite allergic rhinitis. The treatment is effective and safe, and greatly shortens the duration of immune treatment.


Assuntos
Imunoterapia/métodos , Rinite Alérgica/terapia , Animais , Antígenos de Dermatophagoides/administração & dosagem , Humanos , Injeções , Linfonodos , Pescoço , Pyroglyphidae , Qualidade de Vida , Estudos Retrospectivos , Resultado do Tratamento
19.
Int J Mol Sci ; 20(12)2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31234267

RESUMO

The house dust mite (HDM) Dermatophagoides pteronyssinus is an important risk factor for asthma and rhinitis. Allergen specific immunotherapy that is based on recombinant proteins has been proposed for the safer and more efficient treatment of allergic diseases. The aim of this study was to design and obtain a hybrid protein (DPx4) containing antigenic regions of allergens Der p 1, Der p 2, Der p 7, and Der p 10 from this mite. DPx4 was produced in Escherichia coli and its folding was determined by circular dichroism. Non-denaturing dot-blot, ELISA, basophil activation test, dot blot with monoclonal antibodies, ELISA inhibition, and cysteine protease activity assays were performed. Mice that were immunized with DPx4 were also analyzed. We found that DPx4 had no cysteine protease activity and it showed significantly lower IgE reactivity than Der p 1, Der p 2, and D. pteronyssinus extract. DPx4 induced lower basophil activation than Der p 2 and the allergen extract. Immunized mice produced IgG antibodies that inhibited the binding of allergic patient's IgE to the allergen extract and induced comparatively higher levels of IL-10 than the extract in peripheral blood mononuclear cells (PBMC) culture. These results suggest that DPx4 has immunological properties that are useful for the development of a mite allergy vaccine.


Assuntos
Alérgenos/uso terapêutico , Antígenos de Dermatophagoides/uso terapêutico , Dermatophagoides pteronyssinus/imunologia , Hipersensibilidade/prevenção & controle , Alérgenos/genética , Alérgenos/imunologia , Animais , Antígenos de Dermatophagoides/genética , Antígenos de Dermatophagoides/imunologia , Dermatophagoides pteronyssinus/genética , Feminino , Humanos , Hipersensibilidade/imunologia , Imunização , Camundongos , Camundongos Endogâmicos BALB C , Engenharia de Proteínas , Proteínas Recombinantes/genética , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/uso terapêutico , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia , Vacinas Sintéticas/uso terapêutico
20.
BMC Immunol ; 20(1): 18, 2019 06 04.
Artigo em Inglês | MEDLINE | ID: mdl-31164097

RESUMO

BACKGROUND: Mouse models have been extremely valuable in identifying the fundamental mechanisms of airway inflammation that underlie human allergic asthma. Several models are commonly used, employing different methods and routes of sensitisation, and allergens of varying clinical relevance. Although all models elicit similar hallmarks of allergic airway inflammation, including airway eosinophilia, goblet cell hyperplasia and cellular infiltration in lung, it is not established whether they do so by involving the same mechanisms. RESULTS: We compared the impact of inactivation of various innate or adaptive immune genes, as well as sex, in different models of allergic airway inflammation in mice of C57BL/6 background. Chicken ovalbumin (OVA) and house dust mite (HDM) were used as allergens in settings of single or multiple intranasal (i.n.) challenges, after sensitisation in adjuvant or in adjuvant-free conditions. Eosinophil numbers in the broncho-alveolar lavage and lung histopathology were assessed in each model. We found that Major Histocompatibility Complex Class II (MHCII) deficiency and lack of conventional CD4+ T cells had the most profound effect, essentially ablating airway eosinophilia and goblet cell hyperplasia in all models. In contrast, Thymic stromal lymphopoietin receptor (TSLPR) deficiency greatly reduced eosinophilia but had a variable effect on goblet cells. CD1d deficiency and lack of Natural Killer T (NKT) cells moderately impaired inflammation in OVA models but not HDM, whereas sex affected the response to HDM but not OVA. Lastly, defective Toll-like receptor (TLR)4 expression had only a relatively modest overall impact on inflammation. CONCLUSION: All the models studied were comparably dependent on adaptive CD4+ T cell responses and TSLP. In contrast, sex, NKT cells and TLR4 appeared to play subtler and more variable roles that were dependent on the type of allergen and mode of immunization and challenge. These results are consistent with clinical data suggesting a key role of CD4+ T cells and TSLP in patients with allergic asthma.


Assuntos
Alérgenos/imunologia , Eosinofilia/imunologia , Células Caliciformes/patologia , Pulmão/imunologia , Hipersensibilidade Respiratória/imunologia , Imunidade Adaptativa/genética , Adjuvantes Imunológicos , Animais , Antígenos de Dermatophagoides/imunologia , Feminino , Humanos , Imunidade Inata/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Modelos Animais , Ovalbumina/imunologia
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