Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 53.835
Filtrar
1.
Food Chem ; 335: 127576, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-32739805

RESUMO

The main causes of food spoilage come from the process of oxidation and the contamination by microorganisms. For the purpose of increasing food shelf-life the industries employ different techniques, being the addition of preservatives, one of the most used. The aim of this contribution was to investigate the potential antioxidant properties of tyrosol (4-hydroxyphenethyl alcohol, 4-OH) and tyrosol derived isomers (2-hydroxyphenethyl alcohol, 2-OH and 3-hydroxyphenethyl alcohol, 3-OH) against reactive oxygen species (ROS) and the antimicrobial effect on Staphylococcus aureus and Escherichia coli. Furthermore, the type of antioxidant effect of substrates and commercial antioxidants mixtures was studied. Upon visible-light, the substrates interacted with the vitamin B2 and different ROS were generated. All the compounds deactivated O2(1Δg) and O2●-, whereas only 2-OH and 3-OH inhibited H2O2 and HO●. The substrates exhibited a synergistic antioxidant effect when combined with commercial antioxidants. 2-OH showed antimicrobial activity against strains tested.


Assuntos
Aditivos Alimentares/farmacologia , Álcool Feniletílico/análogos & derivados , Riboflavina/farmacologia , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Sinergismo Farmacológico , Peróxido de Hidrogênio/química , Radical Hidroxila/química , Álcool Feniletílico/química , Álcool Feniletílico/farmacologia
2.
Food Chem ; 336: 127744, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32781352

RESUMO

Cardoon (Cynara cardunculus L.) bracts were collected at different maturation stages to investigate seasonal changes in the phenolic compounds profile and in vitro bioactivities. Among the 12 phenolic compounds tentatively identified, 3,5-O-dicaffeoylquinic acid (21.83 mg/g extract) and apigenin-7-O-glucuronide (10.6 mg/g extract) were the most abundant. Immature bracts (C1: principal growth stage (PGS) 5) had the highest phenolic compounds content, and anti-inflammatory (IC50 = 72 µg/mL) and cytotoxic (GI50 of 30-79 µg/mL) activities. Moreover, extract C1 inhibited efficiently the formation of thiobarbituric acid reactive substances (TBARS; IC50 = 26.8 µg/mL), while extract C8 (PGS 8/9) was more effective against oxidative haemolysis (IC50 38 and 75 µg/mL). The highest antibacterial and antifungal activities were attributed to samples C1 and C6 (PGS 7/8) and samples C2 (PGS 5/6) and C4 (PGS 6/7), respectively. Overall, the obtained results suggest the seasonal changes of polyphenolic composition and bioactivity of cardoon bracts of variable maturity.


Assuntos
Cynara/química , Fenóis/química , Animais , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Anti-Inflamatórios/química , Anti-Inflamatórios/farmacologia , Antioxidantes/química , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Cynara/crescimento & desenvolvimento , Cynara/metabolismo , Fungos/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Óxido Nítrico/metabolismo , Fenóis/análise , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Folhas de Planta/metabolismo , Células RAW 264.7 , Estações do Ano
3.
Food Chem ; 336: 127676, 2021 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-32768902

RESUMO

Chicory (Cichorium intybus L.) is a perennial herb from the Cichorium genus, Asteraceae family, and is worldwide cultivated. So far, chicory has been used mainly in animal feed, but also in several cases in the food industry: as salad, for teas and tea blends, for coffee supplementation, and as a source for the inulin production. Nowadays there is an increasing interest in chicory utilization for food production and supplementation. Some compounds present in chicory, such as polyphenols, inulin, oligofructose and sesquiterpene lactones may be considered as potential carriers of food functionality. This review describes nutritional, mineral and bioactive composition of the chicory plant and summarized the main biological activities associated with the presence of bioactive compounds in the different plant parts. Finally, the review explores possibilities of uses of chicory and its implementation in food products, with intention to design new functional foods.


Assuntos
Chicória/química , Ingredientes de Alimentos , Compostos Fitoquímicos/farmacologia , Animais , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Humanos , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Valor Nutritivo , Compostos Fitoquímicos/química , Extratos Vegetais/efeitos adversos , Extratos Vegetais/farmacologia
4.
Food Chem ; 338: 127822, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-32810813

RESUMO

In this study, a potential of covalent linkage approach for developing active edible coatings was examined. Vanillin and trans-cinnamaldehyde were bound to chitosan by Schiff base reaction and reductive amination. The modified polysaccharides were comprehensively characterized and applied as active coatings on fresh-cut melon. The covalent linkage allowed overcoming solubility problems with the lipophilic vanillin and cinnamaldehyde and neutralizing their volatility, producing well-adhered coatings that enhanced fruit quality and storability without sensorial impairment. The attached hydrophobic moieties also provided new polysaccharides with self-assembling ability. Their aggregates were loaded with antimicrobial citral and added to mandarin juice, resulting in up to 6 log CFU/mL microbial count reduction. Thus, the covalent linkage concept offers several advantages, especially when hydrophobic or volatile active agents are used. Further developed, it may become a safe and effective tool for the formation of advanced active edible coatings and delivery vehicles for direct applications on food products.


Assuntos
Acroleína/análogos & derivados , Benzaldeídos/química , Filmes Comestíveis , Polissacarídeos/química , Acroleína/química , Monoterpenos Acíclicos/química , Anti-Infecciosos/química , Quitosana/química , Citrus , Cucurbitaceae/metabolismo , Microbiologia de Alimentos , Conservação de Alimentos/métodos , Qualidade dos Alimentos , Armazenamento de Alimentos , Frutas/metabolismo , Sucos de Frutas e Vegetais/microbiologia , Interações Hidrofóbicas e Hidrofílicas
5.
Sci Total Environ ; 752: 142264, 2021 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-33207511

RESUMO

Green synthesis of silver nanoparticles (Ag NPs) by using plants extracts has provided an eco-friendly alternation for industry and agriculture application. Here, we prepared Ag NPs by using the cucumber leaves and rice husk extracts, and further assessed the antimicrobial activity and phytotoxicity of green synthesized Ag NPs (g-Ag NPs) comparing with chemically synthesized Ag NPs (chem-Ag NPs). The chem-Ag NPs had strong antibacterial activity on the growth of Escherichia coli, while g-Ag NPs by rice husks (gr-Ag NPs) exhibited long-term antibacterial effects. In terms of phytotoxicity, the chem-Ag NPs induced over-generation of ROS and activated plant antioxidant defense systems, thus resulting in the upregulation of MDA and Zn contents and downregulation of antioxidant capacity, carotenoid, globulin and Mo contents. However, g-Ag NPs significantly promoted cucumber photosynthesis by increasing chlorophyll contents. Besides, the green synthesized Ag NPs by cucumber extracts (gc-Ag NPs) increased protein contents and gr-Ag NPs stimulated the upregulation of Mn and the downregulation of Al, which were all positive effects. Overall, compared with chem-Ag NPs, g-Ag NPs exhibited long-tern antimicrobial properties and attenuated toxicity to plants, which could be used as potential nanopesticide or nanoscale growth regulator in agriculture.


Assuntos
Anti-Infecciosos , Nanopartículas Metálicas , Antibacterianos/toxicidade , Química Verde , Nanopartículas Metálicas/toxicidade , Testes de Sensibilidade Microbiana , Extratos Vegetais/toxicidade , Prata/toxicidade
6.
Food Chem ; 334: 127562, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32707368

RESUMO

Brown seaweeds are traditionally used as food in Asian countries, and they are a valuable source of bioactive compounds. Herein, a novel high-throughput methodological approach was developed for the tracing of compounds with radical scavenging and antimicrobial activities in Saccharina japonica and Undaria pinnatifida methanol extracts. The seaweed metabolites were separated by a novel high-performance thin-layer chromatography method, the bioactive bands were identified by bioautography assays. The bioactive compounds were characterized with ultra-high-performance liquid chromatography coupled with linear trap quadrupole tandem mass spectrometry. Stearidonic, eicosapentaenoic, and arachidonic acids were identified as major components having radical scavenging and antimicrobial activities. The suggested method provides a fast identification and quantification of bioactive compounds in multicomponent biological samples.


Assuntos
Anti-Infecciosos/análise , Cromatografia em Camada Delgada/métodos , Alga Marinha/química , Espectrometria de Massas em Tandem/métodos , Anti-Infecciosos/química , Ácidos Araquidônicos/análise , Ácidos Araquidônicos/farmacologia , Ácido Eicosapentaenoico/análise , Ácido Eicosapentaenoico/farmacologia , Ácidos Graxos Ômega-3/análise , Ácidos Graxos Ômega-3/farmacologia , Depuradores de Radicais Livres/química , Depuradores de Radicais Livres/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Ensaios de Triagem em Larga Escala/métodos , Laminaria/química , Laminaria/metabolismo , Alga Marinha/metabolismo , Undaria/química , Undaria/metabolismo
7.
Food Chem ; 334: 127603, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32712492

RESUMO

Present work comprises the use of different solid-state Nuclear Magnetic Resonance strategies for characterizing structural and motional aspects of the peptide matrix that compose a set of four lyophilized Mexican cheese aqueous soluble extracts, each with a controlled ripening. Heteronuclear dipolar coupling modulation schemes allowed to characterize local mobility and structural homogeneity of cheeses' peptide segments in the solid-state as a function of ripening. Results suggest that ripened samples with certain local flexibility but important structural homogeneity present efficient microbial inhibition against tested bacterial strains, whilst high local rigidity of peptides within ripened cheese soluble fractions could partially explain the observed lack of antimicrobial activity. The present study attempts to propose novel observables for lyophilized cheese water soluble extracts that could be partially associated to their ripening-dependent antimicrobial activities, whereas said observables shall contribute to the better targeting, design and optimization of solid-state natural food bio-preservatives.


Assuntos
Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Queijo , Espectroscopia de Ressonância Magnética/métodos , Anti-Infecciosos/análise , Isótopos de Carbono , Queijo/análise , Liofilização , Testes de Sensibilidade Microbiana , Peptídeos/análise , Peptídeos/química , Peptídeos/farmacologia , Solubilidade , Água
8.
Washington, D.C.; OPS; 2020-11-16.
Não convencional em Espanhol | PAHO-IRIS | ID: phr-53020

RESUMO

El presente informe técnico tiene como objeto sustentar los elementos en materia de agua, saneamiento e higiene (WASH, por su sigla en inglés) y de aguas residuales de los planes de acción nacionales multisectoriales que abordan la resistencia a los antimicrobianos. Comprende un resumen de la evidencia y la fundamentación de los beneficios colaterales derivados de las medidas en cada sector y en él además se presenta una serie de medidas para su consideración y perfeccionamiento en el contexto de cada país. Asimismo, contiene opciones de política específicas para cada sector e información adicional, incluidas las lagunas en el conocimiento y las necesidades en el campo de la investigación, así como los recursos técnicos adicionales para apoyar la planificación y la ejecución.


Assuntos
Saneamento , Água , Água Potável , Higiene , Águas Residuárias , Uso de Águas Residuárias , Anti-Infecciosos , Doenças Transmissíveis , Controle de Infecções
9.
Braz Dent J ; 31(5): 485-492, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33146331

RESUMO

Green chemistry has been applied in different areas due to the growing demands for renewable processes and one of them is nanotechnology. The aim of this study was to characterize a formulation containing silver nanoparticles (AgNPs) produced by a green synthesis and to evaluate its antimicrobial activity. The formulation will be used as an intracanal dressing exploiting the AgNPs' antimicrobial properties, which are crucial to prevent infections and bacterial reinfections that can compromise endodontic treatments. In the green synthesis, silver nitrate was employed as the precursor salt, maltose as a reducing agent, and gelatin as a stabilizing agent. The formulation was prepared mixing 50 % of a liquid containing the AgNPs and 50 % of hydroxyethylcellulose gel at 1.5 % with proper evaluation of the process inherent parameters. Techniques such as molecular absorption spectrometry and dynamic light scattering were used in characterization step. The antimicrobial activity of the AgNPs against Escherichia coli ATCC 25922, Enterococcus faecalis NCTC 775, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Streptococcus mutans ATCC 25175 was verified according to National Comittee for Clinical Laboratory Standards (NCCLS) by determining minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The obtained results indicated the formulation containing AgNPs produced by a green synthesis was properly characterized by the selected techniques. Furthermore, the formulation assessment proved that it is suitable for the proposal as well as it has potential to be used as an intracanal dressing since presented antimicrobial activity against all bacterial strains evaluated.


Assuntos
Anti-Infecciosos , Nanopartículas Metálicas , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Bandagens , Testes de Sensibilidade Microbiana , Prata/farmacologia
11.
Artigo em Inglês | MEDLINE | ID: mdl-33151200

RESUMO

This study evaluated the effect of the antimicrobial photodynamic therapy (aPDT) on the osseointegration of immediate implants in a healing situation with greater challenges. The mandibular premolars of eight beagle dogs were submitted to ligature-induced periodontal disease. After 3 months, teeth were extracted and immediate implants were placed in the sockets previously decontaminated by mechanical debridement (MD) or MD+aPDT. Following 12 weeks, the dogs were euthanized and the specimens were analyzed. Histologic and histomorphometric analyses demonstrated significantly better results for the immediate implants decontaminated by debridement associated with aPDT. The sites treated with MD+aPDT led to osseointegration of the immediate implants without evidence of inflammation; conversely, evidence of peri-implantitis was observed where aPDT was not used.


Assuntos
Anti-Infecciosos , Implantes Dentários , Periodontite , Fotoquimioterapia , Animais , Cães , Osseointegração
12.
Zhongguo Zhong Yao Za Zhi ; 45(17): 4238-4245, 2020 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-33164409

RESUMO

The aim of this study was to predict the anti-microbial components in the aerial part of Bupleurum chinense fermented by Lactobacillus plantarum through analyzing the correlation between contents of bioactive components and their inhibitory action for pathogenic bacteria. In this study, the UPLC-MS-MS detection method was established for eight flavonoids(kaempferol-3-O-ß-D-rutinoside, isoquercitrin, quercetin, isorhamnetin, rutin, iridin, quercetin-3-O-ß-L-arabinoside, kaempferol) and DL-3-phenyllactic acid, and the dynamic change of their contents at fermentation course were monitored. Meanwhile, the experiment employed five common no-naquatic pathogenic bacteria(Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Bacillus subtilis), and four common aquatic pathogenic bacteria(Aeruginosa hydrophila, Delayed Edwards, Vibrio alginolyticus, and Vibrio harveyi) to validate in vitro anti-microbial activity of the fermented aerial part of B. chinense at different fermentation time points. Finally, the Pearson correlation analysis was applied to predict the anti-microbial components of the fermented aerial part of B. chinense.The established UPLC-MS-MS method showed a good linearity and the widest linear range was from 0.19 µg·mL~(-1) to 50 µg·mL~(-1). The limit of quantitation and the limit of detection were 0.19-1.56 µg·mL~(-1) and 0.1-0.78 µg·mL~(-1) respectively. During the fermentation within 48 h, the contents of three flavonoids(rutin, quercetin-3-O-ß-L-arabinoside, isoquercitrin) and DL-3-phenyllactic acid from the fermented aerial part of B. chinense increased sharply. In the process of fermentation, the anti-microbial effect of the fermented aerial part of B. chinense on aquatic pathogens was significantly stronger than that on non-aquatic pathogens. Furthermore, Pearson correlation analysis predicted that isoquercitrin, rutin, quercetin-3-O-ß-L-arabinoside and DL-3-phenyllactic acid showed significant correlation with the four aquatic pathogens. This study revealed that the fermented aerial part of B. chinense had a high sensitivity to aquatic pathogens, which may be caused by the increased contents of isoquercitrin, rutin, quercetin-3-O-ß-L-arabinoside and DL-3-phenyllactic acid. In conclusion, this study provides a theoretical basis and new idea for the further development of the large amount of wasteful aerial part of Bupleurum chinense.


Assuntos
Anti-Infecciosos , Bupleurum , Cromatografia Líquida , Componentes Aéreos da Planta , Espectrometria de Massas em Tandem , Vibrio
13.
J Biomed Nanotechnol ; 16(6): 789-809, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-33187577

RESUMO

Biomaterial implants and medical devices have been utilized extensively in medical treatment with the development of modern medicine, especially in orthopaedics and stomatology. Along with their applications, biomaterial-associated infections (BAIs) have grown to be one of the main postoperative complications. Antimicrobial coating strategies have been reported to effectively inhibit bacterial adhesion and proliferation on implant surface, extending their lifespan. In this review, the most topical antimicrobial coating designs have been chosen from literature studies. Their antimicrobial mechanisms and antimicrobial activity assessments in literature studies have been presented and compared. Based on their active ingredients, antimicrobial coatings are categories into (i) inorganic agents, including Ag, Cu, ZnO, MoS2 and nitride compound; (ii) organic agents including antibiotic, antimicrobial peptides, polymer, essential oils etc. The review has provided various and detailed options of antimicrobial coating designs for consulting according to their specific application. It is noted that the research of antimicrobial coatings is mostly in vitro and in vivo animal models study. It is thus in need for more preclinical or clinical studies, especially finding the direct connection between the utilization of antimicrobial coated implants and the reduction in BAIs incidence. Furthermore, future antimicrobial coating designs shall respect also biocompatibility, functionality, and durability apart from their antimicrobial activity.


Assuntos
Anti-Infecciosos , Materiais Revestidos Biocompatíveis , Animais , Antibacterianos/farmacologia , Anti-Infecciosos/farmacologia , Aderência Bacteriana , Próteses e Implantes
14.
J Clin Pediatr Dent ; 44(5): 289-295, 2020 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-33181849

RESUMO

OBJECTIVE: To investigate the effect of two methods of propolis administration on plaque accumulation and microbial count as well as patient acceptance of each vehicle. STUDY DESIGN: A randomized clinical trial with two parallel arms was used with a sample of 60 high caries risk children 6-8 years old. Children were divided randomly into two groups. Group I: Children who received propolis chewing gum and instructed to chew it twice daily for at least twenty minutes, for two weeks. Group II: children who received propolis mouthwash and instructed to rinse twice daily for one minute. A plaque index was recorded and a plaque sample was collected from all participants at base line and after two weeks of treatment. All participants were asked to rate the preparation they received during treatment period on a Visual Analogue Scale chart. RESULTS: Data showed that propolis had a significant effect on reducing plaque scores and colony counts in both vehicles. There was no significant difference between both vehicles neither on plaque reduction nor on microbial count. However children preferred the gum formula. CONCLUSION: Propolis in both vehicles reduced plaque accumulation and microbial count which recommends its use as an antimicrobial agent in different vehicles.


Assuntos
Anti-Infecciosos , Cárie Dentária , Placa Dentária , Própole , Goma de Mascar , Criança , Cárie Dentária/prevenção & controle , Placa Dentária/prevenção & controle , Humanos , Própole/uso terapêutico , Streptococcus mutans
15.
Curr Top Med Chem ; 20(24): 2119-2125, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33153418

RESUMO

Multidrug resistance in microbes poses a major health crisis and demands for the discovery of novel antimicrobial agents. The recent pandemic of SARS-CoV-2 has raised a public health emergency in almost all the countries of the world. Unlike viruses, a bacterium plays a significant role in various environmental issues such as bioremediation. Furthermore, biosurfactants produced by various bacterial species have an edge over traditionally produced chemical surfactants for its biodegradability, low toxicity and better interfacial activity with various applications in agriculture and industry. This special issue focuses on the global perspective of drug discovery for various antimicrobial, antiviral, and antifungal agents for infectious diseases. The issue also emphasizes the ongoing developments and the role of microbes in environmental remediation. We wish the articles published in this issue will enhance the current understanding in microbiology among the readers, and serve as the "seed of an idea" for drug development for ongoing COVID-19 pandemic.


Assuntos
Anti-Infecciosos/farmacologia , Infecções por Coronavirus/tratamento farmacológico , Pneumonia Viral/tratamento farmacológico , Antibacterianos/farmacologia , Antifúngicos/farmacologia , Antivirais/uso terapêutico , Desenvolvimento de Medicamentos , Humanos , Pandemias
16.
BMC Infect Dis ; 20(1): 733, 2020 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-33028262

RESUMO

BACKGROUND: The morbidity and mortality in community-acquired bacterial meningitis (CABM) remain substantial, and the etiology, clinical characteristics, treatment outcomes and predictors of poor prognosis must be assessed regularly. The aim of this study was to identify the distribution of etiological agents and their relationship with clinical characteristics, treatment and outcomes in this cohort of patients with CABM. METHODS: Our retrospective chart review analyzed the causative microorganisms, clinical characteristics, laboratory findings, treatment and outcomes of 159 adults with CABM hospitalized in the Infectious Diseases Centre of Vilnius University Hospital from January 1, 2009 to December 31, 2016. A Glasgow Outcome Scale (GOS) score ≤ 3 was defined as unfavorable outcome. Predictors of an unfavorable outcome were identified through logistic regression analysis. RESULTS: The median patient age was 36 (IQR 24-56), and 51.6% were male. Microbiologically confirmed causative agents were identified in 80 (50.3%) patients: N. meningitidis in 55 (34.6%) patients with serotype B accounting for 85% of cases, S. pneumoniae in 15 (9.4%), L. monocytogenes in 5 (3.1%) and other in 5 (3.1%). The clinical triad of fever, neck stiffness and a change in mental status was present in 59.1% of patients. Coexisting conditions and comorbidities were similar in all groups stratified by etiology. Initial antimicrobial treatment consisted of penicillin in 78 patients (49.1%) and ceftriaxone in 72 patients (45.3%). The median time in which antibiotic treatment was started was 40 min (IQR 30.0-90.0). The outcome was unfavorable in 15.7% of episodes and death occurred in 5.7% of cases and did not differ according to the causative agent. Risk factors for an unfavorable outcome were age > 65 years, coexisting pneumonia and a platelet count <150x10e9/l. CONCLUSIONS: The most common causative agent of CABM was N. meningitidis, with serotype B clearly dominant. Causative agents did not influence the disease outcome. The strongest risk factors for an unfavorable outcome were older age, pneumonia and a low platelet count. Since the introduction of routine vaccination against meningococcus B for infants in Lithuania in 2018, the national vaccination policy may hopefully contribute to a decrease in the incidence of serogroup B meningococcal disease in the Lithuanian population.


Assuntos
Listeria monocytogenes/isolamento & purificação , Meningites Bacterianas/diagnóstico , Neisseria meningitidis/isolamento & purificação , Streptococcus pneumoniae/isolamento & purificação , Adulto , Idoso , Idoso de 80 Anos ou mais , Anti-Infecciosos/uso terapêutico , Comorbidade , Feminino , Humanos , Incidência , Lituânia , Masculino , Meningites Bacterianas/tratamento farmacológico , Meningites Bacterianas/microbiologia , Pessoa de Meia-Idade , Estudos Retrospectivos , Fatores de Risco , Sorogrupo , Resultado do Tratamento , Adulto Jovem
17.
J Indian Soc Pedod Prev Dent ; 38(3): 304-310, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33004730

RESUMO

Background: Despite endodontic infections being a common problem in the primary dentition, some of the infected primary teeth can remain functional until the exfoliation through endodontic treatment. The primary endodontic treatment goal must be to optimize root canal disinfection and to prevent reinfection. Aim: The aim of this study is to compare the antifungal efficacy of antimicrobial photodynamic therapy, LASER, and 1% clotrimazole as irrigants by reducing the colony-forming units (CFU) of Candida albicans in the infected root canals of primary teeth. Materials and Methods: Pulp therapy was performed on deciduous mandibular second molars of 20 healthy children aged 5-8 years, which were divided into four groups of intracanal disinfection protocols: Group 1 (n = 5) - Sodium Hypochlorite, Group 2 (n = 5) - Photodynamic, Group 3 (n = 5) - Normal saline with LASER, and Group 4 - (n = 5) Antifungal. Disinfection potential was compared by collecting samples one just after access opening and the other after the experimental groups using paper points. The samples were cultured and incubated for 48 h to check change in CFU of the fungi. Results: Data were analyzed statistically using the Shapiro-Wilk's test, Mann-Whitney U-test; Kruskal-Wallis test, and Wilcoxon signed-ranks test. No significant difference (P > 0.05) was found between the different groups. However, complete inhibition of C. albicans CFU was seen with Group 4. Conclusion: One percent Clotrimazole (antifungal) can be utilized as an adjunct in the endodontic irrigation protocol for better success of pulpectomy in children.


Assuntos
Anti-Infecciosos , Fotoquimioterapia , Antifúngicos , Candida albicans , Criança , Pré-Escolar , Cavidade Pulpar , Desinfecção , Humanos , Projetos Piloto , Irrigantes do Canal Radicular/uso terapêutico , Preparo de Canal Radicular , Dente Decíduo
19.
Am J Orthod Dentofacial Orthop ; 158(5): e73-e82, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33008710

RESUMO

INTRODUCTION: White spot lesions are a common side effect of orthodontic treatment. This laboratory study aimed to explore the suitability of chlorhexidine hexametaphosphate (CHX-HMP) as a coating for orthodontic elastomeric ligatures to provide sustained chlorhexidine (CHX) release. METHODS: Dissolution kinetics of CHX-HMP were firstly explored using spectroscopy and a colorimetric phosphate assay. Elastomeric ligatures were categorized into 3 groups-acetone-conditioned, ethanol-conditioned, and as received-and were then immersed in 5 mM CHX-HMP suspension or 5 mM chlorhexidine digluconate solution and rinsed. CHX release was measured over 8 weeks, and the effects of conditioning and immersion on elastomeric force and extension at rupture and surface topography were investigated. RESULTS: CHX-HMP exhibited a gradual equilibration that had not reached equilibrium within 8 weeks, releasing soluble CHX and a mixture of polyphosphate and orthophosphate. CHX digluconate-treated ligatures showed no CHX release, whereas CHX-HMP-treated ligatures showed varying degrees of release. As received, CHX-HMP-treated ligatures showed a modest release of CHX up to 7 days. Acetone conditioning did not enhance CHX-HMP uptake or subsequent CHX release and caused a deterioration in mechanical properties. Ethanol conditioning enhanced CHX-HMP uptake (6×) and led to a sustained CHX release over 8 weeks without affecting mechanical properties. CONCLUSIONS: Within the inherent limitations of this in-vitro study, CHX-HMP led to a sustained release of CHX from orthodontic elastomeric ligatures after ethanol conditioning. Conditioned and coated elastomeric ligatures may ultimately find application in the prevention of white spot lesions in orthodontic patients.


Assuntos
Anti-Infecciosos , Clorexidina , Antibacterianos , Humanos , Fosfatos
20.
Carbohydr Polym ; 250: 116800, 2020 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-33049807

RESUMO

Chitosan, as a biodegradable and biocompatible polymer, is characterized by anti-microbial and anti-cancer properties. It lately has received a widespread interest for use as the pulmonary particulate backbone materials of drug carrier for the treatment of infectious disease and cancer. The success of chitosan as pulmonary particulate drug carrier is a critical interplay of their mucoadhesive, permeation enhancement and site/cell-specific attributes. In the case of nanocarriers, various microencapsulation and micro-nano blending systems have been devised to equip them with an appropriate aerodynamic character to enable efficient pulmonary aerosolization and inhalation. The late COVID-19 infection is met with acute respiratory distress syndrome and cancer. Chitosan and its derivatives are found useful in combating HCoV and cancer as a function of their molecular weight, substituent type and its degree of substitution. The interest in chitosan is expected to rise in the next decade from the perspectives of drug delivery in combination with its therapeutic performance.


Assuntos
Anti-Infecciosos/química , Antineoplásicos/química , Quitosana/análogos & derivados , Portadores de Fármacos/química , Anti-Infecciosos/farmacologia , Anti-Infecciosos/uso terapêutico , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Betacoronavirus/isolamento & purificação , Materiais Biocompatíveis/química , Sobrevivência Celular/efeitos dos fármacos , Infecções por Coronavirus/tratamento farmacológico , Infecções por Coronavirus/patologia , Infecções por Coronavirus/virologia , Humanos , Neoplasias Pulmonares/patologia , Pandemias , Pneumonia Viral/tratamento farmacológico , Pneumonia Viral/patologia , Pneumonia Viral/virologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA