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1.
Medicine (Baltimore) ; 99(6): e18854, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32028395

RESUMO

BACKGROUND: The elimination of the pathogenic microorganisms of the periodontal pocket is one of the main points for success in periodontal treatment. The objective of this study is to investigate the clinical and antimicrobial effect of papain-mediated photodynamic therapy in the clinical treatment of periodontal disease. METHODS: Twenty patients with chronic periodontitis will be selected. Patients will be randomly divided into 2 groups (n = 10). Group 1 will receive conventional periodontal treatment and group 2 will receive conventional treatment and antimicrobial photodynamic therapy (PACT). Conventional treatment will consist of oral hygiene guidance, with brushing technique instructions and recommendation of daily flossing. The calculus deposits on the teeth will be removed with ultrasound equipment and curettes for scraping and root planning. The PACT will be performed at the end of each periodontal treatment session, at sites with bags ≥4 mm. PapaMblue photosensitizer will be deposited in the periodontal pockets with a syringe and a pre-irradiation time of 1 minute will be adopted. Then, the laser emitting wavelength of 660 nm, with power of 100 mW, for 2 minutes, radiant exposure of 30 J/cm and power density of 250 mW/cm will be applied. Patients will undergo clinical evaluations before treatment (day 1) at 30, 60, and 90 days after the end of treatment; and microbiological evaluations before and immediately after treatment. The distribution of the data within each group and the homogeneity of the variances will be verified. With this information, the most appropriate statistical test in each evaluation will be used. The sample calculation is based on the literature and the significance level of 5% will be adopted. DISCUSSION: The combination of PACT with methylene blue in a papain gel and the conventional treatment may increase the reduction of bacteria in periodontal pockets.


Assuntos
Anti-Infecciosos/uso terapêutico , Periodontite Crônica/terapia , Azul de Metileno/uso terapêutico , Fármacos Fotossensibilizantes/uso terapêutico , Adulto , Anti-Infecciosos/administração & dosagem , Terapia Combinada , Método Duplo-Cego , Feminino , Humanos , Masculino , Azul de Metileno/administração & dosagem , Fotoquimioterapia , Fármacos Fotossensibilizantes/administração & dosagem , Resultado do Tratamento
3.
Chem Pharm Bull (Tokyo) ; 68(1): 46-57, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31902901

RESUMO

Over the past decade, a number of new 1,4-naphthoquinones have been isolated from natural sources and new 1,4-naphthoquinones with diverse structural features have been synthesized. Cardioprotective, anti-ischemic, hepatoprotective, neuroprotective and some other new properties were found for these compounds; their role in protecting against neurodegenerative diseases has been established. Their anti-inflammatory, antimicrobial and antitumor activities have been studied in more detail; new, previously unknown intracellular molecular targets and mechanisms of action have been discovered. Some compounds of this class are already being used as a medicinal drugs and some substances can be used as biochemical tools and probes for non-invasive detection of pathological areas in cells and tissues in myocardial infarction and neurodegenerative diseases using modern molecular imaging techniques.


Assuntos
Anti-Infecciosos/química , Anti-Inflamatórios/química , Naftoquinonas/química , Substâncias Protetoras/química , Anti-Infecciosos/farmacologia , Anti-Inflamatórios/farmacologia , Bactérias/efeitos dos fármacos , Citocinas/metabolismo , Humanos , Leucócitos Mononucleares/citologia , Leucócitos Mononucleares/efeitos dos fármacos , Leucócitos Mononucleares/metabolismo , Naftoquinonas/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Trypanosoma/efeitos dos fármacos
4.
J Photochem Photobiol B ; 203: 111776, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31931388

RESUMO

Due to the emergence of antibiotic resistance, antimicrobial photodynamic therapy (aPDT) has recently been demonstrated as a promising alternative to antibiotics to treat wound infections caused by multidrug-resistant (MDR) pathogens. This study aimed to evaluate the bacterial killing efficiency of aPDT mediated by methylene blue (MB) loaded thermosensitive hydrogels against methicillin-resistant Staphylococcus aureus (MRSA). Box-Behnken Design method was employed to investigate the impacts of the polymer compositions, Poloxamer 407, Poloxamer 188 and Carbopol 934P, on the gelation temperature (Tsol-gel) and release rate of MB. The viscosity and in vitro bacterial killing efficiency of three selected formulations with Tsol-gel ranged 25-34 °C and MB release in 2 h (the incubation time used for aPDT experiment) ≥ 70%, were assessed. The viscosity was found to increase with increasing P407 content and increasing total gel concentration. In the in vitro aPDT experiment, all tested MB-hydrogels demonstrated >2.5 log10 colony forming unit (CFU) reduction against three clinical relevant MRSA strains. Interestingly, the bacterial reduction increased with decreasing amount of gel added (reduced MB concentration). This was possibly attributed to the increased viscosity at higher gel concentration reducing the diffusion rate of released MB towards bacterial cells leading to reduced aPDT efficiency. In summary, aPDT with the thermosensitive MB hydrogel formulations is a promising treatment strategy for wound infections.


Assuntos
Anti-Infecciosos/química , Hidrogéis/química , Azul de Metileno/química , Anti-Infecciosos/metabolismo , Anti-Infecciosos/farmacologia , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Luz , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Azul de Metileno/metabolismo , Azul de Metileno/farmacologia , Reologia , Temperatura Ambiente , Viscosidade
7.
Chem Commun (Camb) ; 56(3): 356-359, 2020 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-31825398

RESUMO

A facile and scalable strategy for the quick library synthesis of linear-, hinged-, star-, and cyclic-polypeptides with broad-spectrum antimicrobial activity has been reported. The topologically nanoengineered polypeptides show superior antimicrobial activity against Gram-positive and Gram-negative bacteria and low toxicity, allowing screening of architectural polypeptides as mimics of host defense peptides for antimicrobials.


Assuntos
Anti-Infecciosos/síntese química , Peptídeos/química , Animais , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Camundongos , Testes de Sensibilidade Microbiana , Microscopia Confocal , Células NIH 3T3 , Nanotecnologia , Peptídeos/síntese química , Peptídeos/farmacologia
8.
J Photochem Photobiol B ; 202: 111652, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31760374

RESUMO

The Biocompatibility and stability of nanoparticles using plants have been widely investigated due to its applications in the biomedical industry. Currently, there is a growing interest in nanoparticles in bone remodelling. Artemisia annua is an herbal plant commonly used in the treatment of various ailments. This study investigated the zinc oxide nanoparticles (ZnO NPs) using the green synthesis technique from A. annua and the effects of A. annua ZnO-NPs on osteoblast differentiation and inhibition of osteoclast formation. The formulated ZnO-NPs from A. annua were characterized by using various spectroscopic and microscopic methods Fourier transform-infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and UV-Visible spectroscopy. The disc diffusion method was adopted to test the antimicrobial efficacy of ZnO-NPs. The viability of MG-63 cells were assayed by MTT test and Osteogenic-related assays like Real-time PCR and Mineralization assay were adopted to determine the effects of A. annua ZnO-NPs on the multiplication and differentiation of human osteoblast-like MG-63 cells. The characterization of A. annua ZnO-NPs revealed the crystalline nature with high zinc content and the presence of bioactive compounds from A. annua extract. The synthesized A. annua ZnO-NPs indicate significant antimicrobial potential. Besides, A. annua ZnO-NPs enhanced the proliferation, differentiation, and mineralization without causing significant cytotoxic impact on MG-63 cells. These effects indicate that A. annua ZnO-NPs can both stimulate bone formation via the differentiation of MG-63 cells. Hence, it was concluded that A. annua ZnO-NPs can be a promising agent for the treatment of bone deformities and bone-related diseases, however further research also required to explore the clear mechanism of A. annua ZnO-NPs in the formation and differentiation of MG-63 cells.


Assuntos
Artemisia annua/química , Diferenciação Celular , Proliferação de Células , Nanopartículas Metálicas/química , Anti-Infecciosos/síntese química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Artemisia annua/metabolismo , Cálcio/metabolismo , Candida/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Química Verde , Humanos , Nanopartículas Metálicas/toxicidade , Osteoblastos/citologia , Osteoblastos/metabolismo , Osteogênese/efeitos dos fármacos , Casca de Planta/química , Casca de Planta/metabolismo , Extratos Vegetais/química , Óxido de Zinco/química
9.
J Urol ; 203(2): 355-356, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31721674
10.
Vasc Endovascular Surg ; 54(2): 172-174, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31714184

RESUMO

Axillary artery blowout is a rare life- and limb-threatening condition. The traditional surgical approach of ligation and extra-anatomic bypass is associated with a high morbidity and mortality. We present a case report of a 65-year-old male with axillary artery hemorrhage secondary to an irradiated squamous cell cancer. We propose a staged hybrid approach for the treatment of this unusual clinical entity consisting of emergent stent grafting followed by planned elective extra-anatomic bypass, debridement, and a course of specific antimicrobial therapy.


Assuntos
Artéria Axilar/efeitos da radiação , Artéria Axilar/cirurgia , Implante de Prótese Vascular , Carcinoma de Células Escamosas/radioterapia , Procedimentos Endovasculares , Hemorragia/cirurgia , Lesões por Radiação/terapia , Neoplasias Cutâneas/radioterapia , Idoso , Anti-Infecciosos/administração & dosagem , Prótese Vascular , Implante de Prótese Vascular/instrumentação , Carcinoma de Células Escamosas/patologia , Desbridamento , Procedimentos Endovasculares/instrumentação , Hemorragia/diagnóstico , Hemorragia/etiologia , Humanos , Masculino , Lesões por Radiação/diagnóstico , Lesões por Radiação/etiologia , Radioterapia Adjuvante/efeitos adversos , Neoplasias Cutâneas/patologia , Stents , Resultado do Tratamento
11.
Toxicol Lett ; 319: 111-118, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31715245

RESUMO

INTRODUCTION: Silver ion has strong antimicrobial properties and is used in a number of wound dressings. In burn models, silver-nylon dressings produce elevated silver levels in the wound along with minimal systemic effect. We evaluated systemic toxicity in a non-burn wound model to see if a similar pattern of silver ion distribution would occur. METHODS: Eight deep partial-thickness wounds each were created on the dorsum of 40 Gottingen minipigs using a Er-YAG Laser. Half were treated with a 21-day course of silver-nylon dressings (Silverlon®) and half were treated with moist gauze dressings. Wound, blood, liver and kidney silver levels, along with blood chemistry and hematology data were obtained at appropriate intervals. RESULTS: All wounds healed well with healing enhanced by silver-nylon dressings. Silver ion was demonstrable in all wounds treated with silver-nylon at day 21 and after 14 days of no further treatment. Silver ion was not detected in blood, liver or kidney of any animal treated with silver-nylon or control dressings. Liver and kidney function remained normal in all animals. CONCLUSION: A 21-day application of silver-nylon dressings to a non-burn dermal wound produces no systemic or local toxicity in Gottingen minipigs.


Assuntos
Anti-Infecciosos/toxicidade , Bandagens , Prata/toxicidade , Pele/lesões , Animais , Feminino , Masculino , Nylons , Prata/farmacocinética , Suínos , Porco Miniatura , Cicatrização
12.
Odontology ; 108(1): 57-65, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31520265

RESUMO

Dental caries is a type of oral microbiome dysbiosis and biofilm infection that affects oral and systemic conditions. For healthy life expectancy, natural bacteriostatic products are ideal for daily and lifetime use as anti-oral infection agents. This study aimed to evaluate the inhibitory effects of abietic acid, a diterpene derived from pine rosin, on the in vitro growth of cariogenic bacterial species, Streptococcus mutans. The effective minimum inhibitory concentration of abietic acid was determined through observation of S. mutans growth, acidification, and biofilm formation. The inhibitory effects of abietic acid on the bacterial membrane were investigated through the use of in situ viability analysis and scanning electron microscopic analysis. Cytotoxicity of abietic acid was also examined in the context of several human cell lines using tetrazolium reduction assay. Abietic acid was found to inhibit key bacterial growth hallmarks such as colony forming ability, adenosine triphosphate activity (both planktonic and biofilm), acid production, and biofilm formation. Abietic acid was identified as bacteriostatic, and this compound caused minimal damage to the bacterial membrane. This action was different from that of povidone-iodine or cetylpyridinium chloride. Additionally, abietic acid was significantly less cytotoxic compared to povidone-iodine, and it exerted lower toxicity towards epithelial cells and fibroblasts compared to that against monocytic cells. These data suggest that abietic acid may prove useful as an antibacterial and antibiofilm agent for controlling S. mutans infection.


Assuntos
Anti-Infecciosos , Cárie Dentária , Antibacterianos , Biofilmes , Humanos , Testes de Sensibilidade Microbiana , Streptococcus mutans
13.
Eur J Dent Educ ; 24(1): 88-94, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31574199

RESUMO

INTRODUCTION: The objective of this study was to assess perceptions and attitudes amongst dental practitioners in relation to antibiotic usage and antibiotic resistance. METHODS: Self-administered questionnaire was given to dental practitioners employed in south Croatia, west Herzegovina and Sarajevo, Bosnia and Herzegovina (N = 115). RESULTS: 81.7% of respondents agreed the usage of antimicrobials is frequently uncritical and unnecessary. 83.5% of dental practitioners reported that they have used guidelines in their practice; however, only 9 out of 115 stated valid guidelines. One-third of the respondents agreed or were undecided that the usage of antimicrobials in every oral inflammatory process treatment is justified. Furthermore, 13% was undecided and 26% agreed that pregnant women and breastfeeding women should not use any antimicrobials. However, three quarters of respondents considered they had satisfactory knowledge on antimicrobials. DISCUSSION: The respondents considered they had satisfactory knowledge on antimicrobials, which was in contrast to the knowledge shown, but also expressed the need for additional education. Therefore, adequate measures include the creation of the local guidelines, their implementation, and updating the practitioners' knowledge on antibiotic use and resistance through continuous educational courses.


Assuntos
Anti-Infecciosos , Odontólogos , Antibacterianos , Bósnia e Herzegóvina , Croácia , Feminino , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Gravidez , Inquéritos e Questionários
14.
Odontology ; 108(1): 34-42, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31214896

RESUMO

Fucoidans are sulfated polysaccharides that are found in marine algae and have many useful activities, including antitumor effects, promotion of apoptosis of cancer cells, and antiviral, anti-inflammatory, and antiallergic actions. In oral medicine, several case reports have shown that fucoidan-containing creams and tablets markedly improved recurrent aphthous stomatitis, symptomatic inflamed tongue, and recurrent oral herpes labialis. The aim of this study was to examine the properties of fucoidans for use in oral healthcare. The antimicrobial, anti-adhesion, endotoxin-neutralizing, and cyclooxygenase (COX)-1 and COX-2 inhibitory activities of fucoidans were examined. Four key results were obtained: fucoidans showed strong antimicrobial activity against Candida albicans, Streptococcus mutans, and Porphyromonas gingivalis; significantly inhibited the adhesion of S. mutans to bovine teeth and porcelain; were suggested to bind to and neutralize endotoxin (lipopolysaccharide) in an LAL assay; and showed COX-1 and/or COX-2 inhibitory activity. These results suggested that fucoidans may be useful in the field of oral healthcare.


Assuntos
Anti-Infecciosos , Polissacarídeos , Animais , Bovinos , Porphyromonas gingivalis , Streptococcus mutans
15.
Sci Total Environ ; 700: 134443, 2020 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-31655455

RESUMO

Worldwide, billions of people are at risk from viruses, parasites and bacteria transmitted by mosquitoes, ticks, fleas and other vectors. Over exploitation of chemical pesticides to overcome the mosquito borne diseases led to detrimental effects on environment and human health. The present research aims to explore bio-fabrication of metal silver nanomaterials using Rhazya stricta extract against deadly mosquito vectors and microbial pathogens. The phytochemical profile of the R. stricta extracts was studied by HPLC-MS and 1H NMR. Further, confirmation of the bio-fabricated silver nanoparticles (AgNPs) was carried out by UV-vis spectroscopy and characterization through FTIR, TEM, EDX, and XRD analyses. The R. stricta-fabricated AgNPs showed acute toxicity on key mosquito vectors from two different country (India and Kingdom of Saudi Arabia, KSA) strains, notably, with LC50 values of 10.57, 11.89 and 12.78 µg/ml on malarial, dengue and filarial key Indian strains of mosquito vectors, respectively, and 30.66 and 38.39 µg/ml on KSA strains of Aedes aegypti and Culex pipiens, respectively. In mosquito adulticidal activity, R. stricta extract alone exhibited LC50 values ranging from 304.34 to 382.45 µg/ml against Indian strains and from 738.733 to 886.886 against Saudi Arabian strains, while AgNPs LC50 boosted from 9.52 to 12.16 µg/ml and from 30.66 to 38.39 µg/ml, respectively. Moreover, it was noticed that at low concentration the tested AgNPs showed high growth retardation of important pathogenic bacteria such as Bacillus subtilis, Klebsiella pneumoniae and Salmonella typhi with inhibition zone diameters from 11.86 to 22.92 mm. In conclusion, the present study highlighted that R. stricta-fabricated AgNPs could be a lead material for the management of mosquito vector and microbial pathogens control.


Assuntos
Anti-Infecciosos/química , Apocynaceae , Inseticidas/química , Nanopartículas Metálicas/química , Mosquitos Vetores/efeitos dos fármacos , Extratos Vegetais/química , Prata/química , Anti-Infecciosos/toxicidade , Índia , Inseticidas/toxicidade , Dose Letal Mediana , Controle de Mosquitos/métodos , Extratos Vegetais/toxicidade
16.
Gen Dent ; 68(1): 41-44, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31859661

RESUMO

The objective of this study was to evaluate the antimicrobial capacity of glycolic acid (GA) at different concentrations as a final irrigant during the preparation of root canals. The sample consisted of 77 extracted single-rooted human teeth with complete root formation, no previous endodontic treatment, and a root length of at least 14 mm. The root canals were prepared in a standardized manner with a rotary file system. During this process, irrigation was performed with 2.5% sodium hypochlorite (NaOCl), and the final irrigant was 17% ethylenediaminetetraacetic acid (EDTA). After the root canal sterilization procedure, Enterococcus faecalis was cultured in a Petri dish, and 70 sterilized root canals were inoculated with a suspension containing 3.0 × 108 colony-forming units (CFUs) per milliliter. The roots were divided into 7 groups (n = 10) according to the following solutions: 0.9% sodium chloride (NaCl); 6% NaOCl; 17% EDTA; 10%, 17%, or 25% GA; or 17% citric acid (CA). The capacity of the different substances to reduce E faecalis was evaluated by counting the CFUs before and after treatment with the final irrigant solutions. Data were subjected to an analysis of variance and the Tukey test at a 5% significance level. The greatest bacterial reduction was observed in the group irrigated with NaOCl (P < 0.05). There were no statistically significant differences among the groups irrigated with GA in different concentrations (P > 0.05), but they all demonstrated greater disinfection capacity than CA and EDTA (P < 0.05). CA showed significantly greater antimicrobial capacity than EDTA (P < 0.05). EDTA showed significantly greater antimicrobial capacity only in relation to NaCl (P < 0.05). At different concentrations, GA demonstrated greater capacity to eliminate E faecalis from root canals than did EDTA.


Assuntos
Anti-Infecciosos , Glicolatos , Irrigantes do Canal Radicular , Preparo de Canal Radicular , Anti-Infecciosos/farmacologia , Cavidade Pulpar , Ácido Edético , Enterococcus faecalis , Glicolatos/farmacologia , Humanos , Preparo de Canal Radicular/métodos , Hipoclorito de Sódio
17.
J Enzyme Inhib Med Chem ; 35(1): 59-64, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31663383

RESUMO

A primary strategy to combat antimicrobial resistance is the identification of novel therapeutic targets and anti-infectives with alternative mechanisms of action. The inhibition of the metalloenzymes carbonic anhydrases (CAs, EC 4.2.1.1) from pathogens (bacteria, fungi, and protozoa) was shown to produce an impairment of the microorganism growth and virulence. As phosphonamidates have been recently validated as human α-CA inhibitors (CAIs) and no phosphorus-based zinc-binding group have been assessed to date against ß-class CAs, herein we report an inhibition study with this class of compounds against ß-CAs from pathogenic bacteria, fungi, and protozoa. Our data suggest that phosphonamidates are among the CAIs with the best selectivity for ß-class over human isozymes, making them interesting leads for the development of new anti-infectives.


Assuntos
Amidas/farmacologia , Anti-Infecciosos/farmacologia , Inibidores da Anidrase Carbônica/farmacologia , Anidrases Carbônicas/metabolismo , Compostos Organometálicos/farmacologia , Ácidos Fosfóricos/farmacologia , Amidas/química , Anti-Infecciosos/síntese química , Anti-Infecciosos/química , Bactérias/efeitos dos fármacos , Bactérias/enzimologia , Inibidores da Anidrase Carbônica/síntese química , Inibidores da Anidrase Carbônica/química , Relação Dose-Resposta a Droga , Fungos/efeitos dos fármacos , Fungos/enzimologia , Humanos , Leishmania donovani/efeitos dos fármacos , Leishmania donovani/enzimologia , Estrutura Molecular , Compostos Organometálicos/síntese química , Compostos Organometálicos/química , Ácidos Fosfóricos/química , Fósforo/química , Fósforo/farmacologia , Relação Estrutura-Atividade , Zinco/química , Zinco/farmacologia
18.
Chemosphere ; 239: 124736, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31494326

RESUMO

In this work, a novel process involving the preparation of nanochitosan-grafted flocculants (CPAM-g-NCS) to treat low turbid and salmonella suspensions simultaneously was introduced. Nanotechnology was employed to enhance the adsorption-adhesion and sterilization abilities of dual-functional flocculants. The monomers of chitosan, acrylamide, methacryloyl ethyl trimethyl ammonium chloride, and sodium tripolyphosphate were utilized for flocculants copolymerization. Then, using fourier-transform infrared spectroscopy, nuclear magnetic resonance hydrogen spectrum, and thermogravimetric and differential scanning calorimetry analysis, the successful synthesis of CPAM-g-NCS was verified. Scanning electron microscopy and size analysis suggested that nanostructured flocculants with irregular morphology and nanocolloids of 60.44 nm were formed. CPAM-g-NCS was applied to treat a series of simulated low turbid and salmonella suspensions. The simulation results showed that the minimum residual turbidity of 1.97 NTU and optical density of 0.16 (initial 0.89) can be achieved at dosages of 2.5 and 8.75 mg L-1, respectively, which were superior to conventional organics flocculants. Mechanistic studies suggested that the excellent adsorption property, and large numbers of quaternary ammonium and amino groups of nanoflocculants contributed to the superior flocculation and antibacterial performance of CPAM-g-NCS.


Assuntos
Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Nanoestruturas/química , Purificação da Água/métodos , Acrilamida/química , Varredura Diferencial de Calorimetria , Quitosana/química , Floculação , Espectroscopia de Ressonância Magnética , Metacrilatos/química , Microscopia Eletrônica de Varredura , Polimerização , Salmonella typhimurium/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Suspensões , Termogravimetria , Microbiologia da Água
19.
Crit Rev Food Sci Nutr ; 60(2): 310-321, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-30431327

RESUMO

Bakery products, as an important part of a healthy diet, are characterized by their limited shelf-life. Microbiological spoilage of these products not only affects the quality characteristics and result in the economic loss but also threatens consumer's health. Incorporation of chemical preservatives, as one of the most conventional preserving techniques, lost its popularity due to the increasing consumer's health awareness. Therefore, the bakery industry is seeking alternatives to harmful antimicrobial agents that can be accepted by health-conscious customers. In this regard, essential oils have been previously used as either a part of product ingredient or a part of the packaging system. Therefore, the antimicrobial aspect of essential oils and their ability in delaying the microbiological spoilage of bakery products have been reviewed. Several types of essential oils, including thyme, cinnamon, oregano, and lemongrass, can inhibit the growth of harmful microorganisms in bakery products, resulting in a product with extended shelf-life and enhanced safety. Research revealed that several bioactive compounds are involved in the antimicrobial activity of essential oils. However, some limitations, such as the possible negative effects of essential oils on sensory parameters, may limit their applications, especially in high concentrations. In this case, they can be used in combination with other preservation techniques such as using appropriate packaging materials. Further research regarding the commercial production of the bakery products formulated with essential oils is required in this area.


Assuntos
Conservação de Alimentos , Óleos Voláteis , Origanum , Anti-Infecciosos , Microbiologia de Alimentos , Conservantes de Alimentos , Thymus (Planta)
20.
Chemistry ; 26(8): 1880-1886, 2020 Feb 06.
Artigo em Inglês | MEDLINE | ID: mdl-31868256

RESUMO

N-(4-Nitrobenzoyl)-Phe self-assembled into a transparent supramolecular hydrogel, which displayed high fibroblast and keratinocyte cell viability. The compound showed a mild antimicrobial activity against E. coli both as a hydrogel and in solution. Single-crystal XRD data revealed packing details, including protonation of the C-terminus due to an apparent pKa shift, as confirmed by pH titrations. MicroRaman analysis revealed almost identical features between the gel and crystal states, although more disorder in the former. The hydrogel is thermoreversible and disassembles within a range of temperatures that can be fine-tuned by experimental conditions, such as gelator concentration. At the minimum gelling concentration of 0.63 wt %, the hydrogel disassembles in a physiological temperature range of 39-42 °C, thus opening the way to its potential use as a biomaterial.


Assuntos
Anti-Infecciosos/química , Materiais Biocompatíveis/química , Hidrogéis/química , Aminoácidos/química , Animais , Anti-Infecciosos/farmacologia , Materiais Biocompatíveis/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Dicroísmo Circular , Cristalografia por Raios X , Escherichia coli/efeitos dos fármacos , Humanos , Camundongos , Conformação Molecular , Células NIH 3T3
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