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1.
PLoS One ; 15(8): e0237271, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32866161

RESUMO

Molecular separation of pharmaceutical contaminants from water has been recently of great interest to alleviate their detrimental impacts on environment and human well-being. As the novelty, this investigation aims to develop a mechanistic modeling approach and consequently its related CFD-based simulations to evaluate the molecular separation efficiency of ibuprofen (IP) and its metabolite 4-isobutylacetophenone (4-IBAP) from water inside a porous membrane contactor (PMC). For this purpose, octanol has been applied as an organic phase to extract IP and 4-IBAP from the aqueous solution due to high solubility of solutes in octanol. Finite element (FE) technique is used as a promising tool to simultaneously solve continuity and Navier-Stokes equations and their associated boundary conditions in tube, shell and porous membrane compartments of the PMC. The results demonstrated that the application of PMC and liquid-liquid extraction process can be significantly effective due to separating 51 and 54% of inlet IP and 4-IBAP molecules from aqueous solution, respectively. Moreover, the impact of various operational / functional parameters such as packing density, the number of fibrous membrane, the module length, the membrane porosity / tortuosity, and ultimately the aqueous solution flow rate on the molecular separation efficiency of IP and 4-IBAP is studied in more details.


Assuntos
Acetofenonas/isolamento & purificação , Anti-Inflamatórios não Esteroides/isolamento & purificação , Ibuprofeno/isolamento & purificação , Membranas Artificiais , Polímeros/química , Extração Líquido-Líquido/métodos , Octanóis/química , Porosidade , Solubilidade , Soluções
2.
PLoS One ; 15(8): e0236297, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32780750

RESUMO

Naproxen is a widely used non-steroidal anti-inflammatory drug for the control of postoperative inflammatory signs and symptoms in dentistry. Its association with esomeprazole has been widely studied and has yielded good results for the control of acute pain, even with the delayed absorption of naproxen owing to the presence of esomeprazole. To further understand the absorption, distribution, and metabolism of this drug alone and in combination with esomeprazole, we will analyze the pharmacokinetic parameters of naproxen and its major metabolite, 6-O-desmethylnaproxen, in saliva samples. A rapid, sensitive, and selective liquid chromatography-tandem mass spectrometric method for the simultaneous determination of naproxen and 6-O-desmethylnaproxen in saliva will be developed and validated. Sequential saliva samples from six patients will be analyzed before and 0.25, 0.5, 0.75, 1, 1.5, 2, 3, 4, 5, 6 8, 11, 24, 48, 72, and 96 h after the ingestion of one naproxen tablet (500 mg) and esomeprazole-associated naproxen tablets (500 + 20 mg), at two different times. After liquid-liquid extraction with ethyl acetate and HCl, the samples will be analyzed using an 8040 Triple Quadrupole Mass Spectrometer (Shimadzu, Kyoto, Japan). Separation of naproxen and its major metabolic products will be performed using a Shim-Pack XR-ODS 75Lx2.0 column and C18 pre-column (Shimadzu, Kyoto, Japan) at 40°C using a mixture of methanol and 10 mM ammonium acetate (70:30, v/v) with an injection flow of 0.3 mL/min. The total analytical run time will be 5 min. The detection and quantification of naproxen and its metabolite will be validated, which elucidate the pharmacokinetics of this drug, thereby contributing to its proper prescription for the medical and dental interventions that cause acute pain.


Assuntos
Anti-Inflamatórios não Esteroides/farmacocinética , Monitoramento de Medicamentos/métodos , Esomeprazol/farmacocinética , Naproxeno/análogos & derivados , Saliva/química , Administração Oral , Adolescente , Adulto , Anti-Inflamatórios não Esteroides/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Esomeprazol/administração & dosagem , Esomeprazol/isolamento & purificação , Feminino , Absorção Gastrointestinal , Humanos , Masculino , Metanol/química , Pessoa de Meia-Idade , Naproxeno/administração & dosagem , Naproxeno/isolamento & purificação , Naproxeno/farmacocinética , Dor Processual/tratamento farmacológico , Reprodutibilidade dos Testes , Comprimidos , Espectrometria de Massas em Tandem/métodos , Adulto Jovem
3.
Artigo em Inglês | MEDLINE | ID: mdl-32466088

RESUMO

In view of the valorisation of the green microalga Scenedesmus obliquus biomass, it was used for the biosorption of two nonsteroidal anti-inflammatory drugs, namely salicylic acid and ibuprofen, from water. Microalgae biomass was characterized, namely by the determination of the point of zero charge (pHPZC), by Fourier transform infrared (FT-IR) analysis, simultaneous thermal analysis (STA) and scanning electron microscopy with energy dispersive spectroscopy (SEM/EDS). Kinetic and equilibrium batch experiments were carried out and results were found to fit the pseudo-second order equation and the Langmuir isotherm model, respectively. The Langmuir maximum capacity determined for salicylic acid (63 mg g-1) was larger than for ibuprofen (12 mg g-1), which was also verified for a commercial activated carbon used as reference (with capacities of 250 and 147 mg g-1, respectively). For both pharmaceuticals, the determination of thermodynamic parameters allowed us to infer that adsorption onto microalgae biomass was spontaneous, favourable and exothermic. Furthermore, based on the biomass characterization after adsorption and energy associated with the process, it was deduced that the removal of salicylic acid and ibuprofen by Scenedesmus obliquus biomass occurred by physical interaction.


Assuntos
Anti-Inflamatórios não Esteroides , Microalgas , Scenedesmus , Poluentes Químicos da Água , Adsorção , Anti-Inflamatórios não Esteroides/isolamento & purificação , Biomassa , Concentração de Íons de Hidrogênio , Cinética , Espectroscopia de Infravermelho com Transformada de Fourier , Termodinâmica , Poluentes Químicos da Água/isolamento & purificação
4.
Food Chem ; 328: 127097, 2020 Oct 30.
Artigo em Inglês | MEDLINE | ID: mdl-32470774

RESUMO

In this study, polyaniline modified polyacrylonitrile nanofibers mat (PANI NFsM) was prepared as a novel adsorbent for the solid-phase extraction (SPE) of non-steroidal anti-inflammatory drug residues in meat or egg samples. The solvent extracts of samples were simply diluted with water to perform the SPE, and then the eluent was directly analyzed. Significant reduction of the matrix effect was obtained after SPE using only 5 mg of PANI NFsM. The entire sample preparation time is 5-10 times lower than the existing methods. The limits of detection of the target analytes ranging from 0.6 to 12.2 µg kg-1 had already met the demand of food safety monitoring by only 1 g sample. The recoveries ranged from 85.18% to 107.31%, with the intra-day and inter-day relative standard deviations of 2.74% to 16.01%, revealing satisfactory accuracy and precision. Finally, real samples analyses were applied to verify the practicability of the method.


Assuntos
Compostos de Anilina/química , Ração Animal/análise , Resíduos de Drogas/análise , Resíduos de Drogas/isolamento & purificação , Nanofibras/química , Extração em Fase Sólida/métodos , Resinas Acrílicas/química , Animais , Anti-Inflamatórios não Esteroides/análise , Anti-Inflamatórios não Esteroides/isolamento & purificação , Química Verde , Limite de Detecção , Fatores de Tempo
5.
Phytochemistry ; 171: 112231, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31901473

RESUMO

Daphne giraldii Nitsche., a member of the genus Daphne (Thymelaeaceae), is a deciduous shrub with mild toxicity. Its rhizome bark, generally called 'Zushima' in Chinese, has many medicinal folkloric uses and good therapeutic effects. Previous studies investigating the chemical constituents and pharmacological activities of D. giraldii have focused on several major classes of compounds, such as coumarins, lignans and flavonoids, especially the interesting enantiomeric flavans. Extracts and pure compounds of D. giraldii were found to possess anti-inflammatory, anti-nociceptive, cytotoxicity, antimalarial, immunomodulating, sedative and hypnotic effects. They have also been reported to influence the cardiovascular functions and blood activities. This comprehensive review will describe the advances in the phytochemistry, pharmacology, medicinal uses and clinical applications of D. giraldii and its formulations covering the literature published from 1970 to 2018. Almost half of the reviewed studies were originally published in non-English languages (mainly in Chinese). Collectively, the aim of this article is to open new avenues for further in-depth pharmacological studies on D. giraldii.


Assuntos
Compostos Fitoquímicos/farmacologia , Thymelaeaceae/química , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Anti-Inflamatórios não Esteroides/farmacologia , Fármacos Antiobesidade/química , Fármacos Antiobesidade/isolamento & purificação , Fármacos Antiobesidade/farmacologia , Antimaláricos/química , Antimaláricos/isolamento & purificação , Antimaláricos/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Humanos , Hipnóticos e Sedativos/química , Hipnóticos e Sedativos/isolamento & purificação , Hipnóticos e Sedativos/farmacologia , Fatores Imunológicos/química , Fatores Imunológicos/isolamento & purificação , Fatores Imunológicos/farmacologia , Compostos Fitoquímicos/química , Compostos Fitoquímicos/isolamento & purificação
6.
Artigo em Inglês | MEDLINE | ID: mdl-31973074

RESUMO

In this study, we analyzed 27 pharmaceuticals in liquid and solid phase samples collected from the unit processes of four different sewage treatment plants (STPs) to evaluate their distribution and behavior of the pharmaceuticals. The examination of the relative distributions of various categories of pharmaceuticals in the influent showed that non-steroidal anti-inflammatory drugs (NSAIDs) were the most dominant. While the relative distribution of antibiotics in the influent was not high (i.e., 3%-5%), it increased to 14%-30% in the effluent. In the four STPs, the mass load of the target pharmaceuticals was reduced by 88%-95% mainly in the biological treatment process, whereas the ratio of pharmaceuticals in waste sludge to those in the influent (w/w) was only 2%. In all the STPs, the removal efficiencies for the stimulant caffeine, NSAIDs (acetaminophen, naproxen, and acetylsalicylic acid), and the antibiotic cefradine were high; they were removed mainly by biological processes. Certain compounds, such as the NSAID ketoprofen, contrast agent iopromide, lipid regulator gemfibrozil, and antibiotic sulfamethoxazole, showed varying removal efficiencies depending on the contribution of biodegradation and sludge sorption. In addition, a quantitative meta-analysis was performed to compare the pharmaceutical removal efficiencies of the biological treatment processes in the four STPs, which were a membrane bioreactor (MBR) process, sequencing batch reactor (SBR) process, anaerobic-anoxic-oxic (A2O) process, and moving-bed biofilm reactor (MBBR) process. Among the biological processes, the removal efficiency was in the order of MBR > SBR > A2O > MBBR. Among the tertiary treatment processes investigated, powdered activated carbon showed the highest removal efficiency of 18%-63% for gemfibrozil, ibuprofen, ketoprofen, atenolol, cimetidine, and trimethoprim.


Assuntos
Preparações Farmacêuticas/isolamento & purificação , Esgotos , Eliminação de Resíduos Líquidos , Poluentes Químicos da Água/isolamento & purificação , Anti-Inflamatórios não Esteroides/isolamento & purificação , Biodegradação Ambiental , Biofilmes , Reatores Biológicos
7.
J Chromatogr A ; 1614: 460718, 2020 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-31787265

RESUMO

In the present work, NiFe layered double hydroxide (LDH)/Nylon 6 composite nanofibers were prepared by electrospinning method and used as a new sorbent for the extraction and measurement of non-steroidal anti-inflammatory drugs (naproxen, mefenamic acid, and diclofenac) in whole blood samples. The method is based on micro solid phase extraction (µSPE) by packed sorbent followed by HPLC-UV analysis. Effective parameters on the extraction efficiency were optimized using a central composite design (CCD). In order to characterize the sorbent, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), field emission scanning electron microscopy (FESEM), energy dispersive X-ray analysis (EDX) and elemental mapping were applied. The method was fully validated based on linearity, limits of detection (LOD) and quantification (LOQ), precision, and recovery. Under the optimal conditions, LOD values were found to be 25 ng mL-1 for naproxen and diclofenac and 15 ng mL-1 for mefenamic acid. A seven-point calibration curve was obtained in the range of 75-2000 ng mL-1 for naproxen and diclofenac and 50-2000 for mefenamic acid. The method showed good linearity with coefficients of determination, r2> 0.9962, for the three drugs. In the entire analytical range, the relative standard deviations (RSD%) were less than 8.1%. Finally, the efficiency of the method was investigated for the analysis of the target analytes in human blood samples, and the recoveries were obtained in the range of 90.7-109.8%.


Assuntos
Anti-Inflamatórios não Esteroides/sangue , Nanofibras/química , Microextração em Fase Sólida/métodos , Anti-Inflamatórios não Esteroides/isolamento & purificação , Caprolactama/análogos & derivados , Cromatografia Líquida de Alta Pressão , Diclofenaco/sangue , Diclofenaco/isolamento & purificação , Humanos , Hidróxidos/química , Ferro , Limite de Detecção , Ácido Mefenâmico/sangue , Ácido Mefenâmico/isolamento & purificação , Naproxeno/sangue , Naproxeno/isolamento & purificação , Níquel , Polímeros
8.
Phytochemistry ; 170: 112191, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31731236

RESUMO

Seven undescribed polyketides javanicols A-E, 5-epi-citreoviridin and 5-epi-isocitreoviridin, together with five known compounds, were isolated from the endolichenic fungus Eupenicillium javanicum. The structures of these polyketides were determined by means of extensive spectroscopic analyses, electronic circular dichroism (ECD) calculations and gauge-independent atomic orbital (GIAO) NMR shift calculations. These compounds were evaluated for potential anti-inflammatory activity against LPS-activated RAW 264.7 cells. Javanicol E and (+)-terrein displayed moderate inhibitory effects on NO production, with IC50 values of 17.00 and 13.46 µM, respectively.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Eupenicillium/química , Óxido Nítrico/antagonistas & inibidores , Policetídeos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Óxido Nítrico/biossíntese , Compostos Fitoquímicos , Policetídeos/química , Policetídeos/isolamento & purificação , Células RAW 264.7
9.
Mikrochim Acta ; 186(12): 759, 2019 11 11.
Artigo em Inglês | MEDLINE | ID: mdl-31712934

RESUMO

A (glycidyl methacrylate)-co-(ethylene glycol dimethacrylate) polymer (poly(GMA-co-EDMA)) was functionalized with metal-organic frameworks (MOF) and used as a sorbent for solid-phase extraction (SPE). The polymeric sorbent was prepared in-situ by photopolymerization in a previously wall-modified spin column, and then modified with an amino-modified MOF of type NH2-MIL-101(Cr). The sorbents were used for the extraction of nonsteroidal anti-inflammatory drugs (NSAIDs) from human urine samples. The sorbent was compared with the parent monolith and embedded approach, where the MOF particles are admixed in the polymerization mixture before the in-situ polymerization in the modified spin column. SPE is performed by percolating the sample solutions in a centrifuge, which streamlines the SPE steps. The hybrid composites were characterized by scanning electron microscopy and nitrogen intrusion porosimetry. Three NSAIDs (ketoprofen, flurbiprofen, and ibuprofen) were tested. They were eluted from the sorbent with acidified water-acetonitrile mixtures and subsequently analyzed by reversed-phase HPLC with UV detection. The detection limits varied in the range from 0.1 to 7 µg·L-1, and the precisions (relative standard deviation) were <14% in all the cases. The recoveries were between 71.0 and 78.0% in spiked urine samples. Graphical abstractA hybrid monolith modified with amino-modified MOF [named NH2-MIL-101(Cr)] in wall-modified spin columns was prepared. The resulting micro-extraction device was applied to the extraction and preconcentration of non-steroidal anti-inflammatory drugs.


Assuntos
Anti-Inflamatórios não Esteroides/isolamento & purificação , Flurbiprofeno/isolamento & purificação , Ibuprofeno/isolamento & purificação , Cetoprofeno/isolamento & purificação , Estruturas Metalorgânicas/química , Microextração em Fase Sólida/métodos , Adsorção , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/urina , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Flurbiprofeno/química , Flurbiprofeno/urina , Humanos , Ibuprofeno/química , Ibuprofeno/urina , Cetoprofeno/química , Cetoprofeno/urina , Limite de Detecção , Metilmetacrilatos/química
10.
Mikrochim Acta ; 186(11): 710, 2019 10 24.
Artigo em Inglês | MEDLINE | ID: mdl-31650257

RESUMO

Polycaprolactone composite nanofibers coated with a polydopamine layer are introduced as a new type of absorption material for on-line solid phase extraction (SPE) in chromatographic system. A hybrid technology combining the electrospinning and melt blowing was used for the preparation of 3D-structured microfiber/nanofibrous polycaprolactone composite. The dopamine coating was then applied to functionalize the micro/nanofibers. Polydopamine-coated polycaprolactone fibers were tested as an extraction phase in on-line SPE prior to HPLC separation and UV detection. Four groups of biologically active substances including bisphenols (Bisphenol S, Bisphenol AF, Bisphenol A, Bisphenol C, Bisphenol AP, Bisphenol Z, Bisphenol BP, and Bisphenol M), betablockers (Timolol, Metoprolol, Labetalol, and Propranolol), nonsteroidal antiphlogistic drugs (Salicylic acid, Ketoprofen, Naproxen, Indomethacin, Diclofenac, Ibuprophen, and Meclofenamic acid), and phenolic acids (Chlorogenic acid, Caffeic acid, Sinapic acid, m-Coumaric acid, Benzoic acid, and Cinnamic acid) were used as the model analytes. Neat and coated fibers were compared and applied as sorbents for the on-line extraction set-up. Both materials produced good extraction potential for the determination of bisphenols and nonsteroidal drugs in model biological and environmental samples including river water, human urine, and blood serum. However, the polydopamine layer significantly increased the extraction efficiency of polar drugs. Typical repeatability of on-line extraction procedure on polydopamine coated fibers was in the range 0.12-4.11% for bisphenols, 0.55-1.41% for antiphlogistic drugs, 0.59-2.52% for phenolic acids, and 1.01-1.65% for betablockers. Graphical abstract Schematic representation of polycaprolactone composite nanofibers coated with a polydopamine layer as an advanced absorption material for on-line solid phase extraction in chromatography.


Assuntos
Antagonistas Adrenérgicos beta/isolamento & purificação , Anti-Inflamatórios não Esteroides/isolamento & purificação , Indóis/química , Nanofibras/química , Fenóis/isolamento & purificação , Poliésteres/química , Polímeros/química , Antagonistas Adrenérgicos beta/análise , Anti-Inflamatórios não Esteroides/análise , Cromatografia Líquida de Alta Pressão , Cinamatos/análise , Cinamatos/isolamento & purificação , Fenóis/análise , Polimerização , Reprodutibilidade dos Testes , Rios/química , Extração em Fase Sólida/métodos , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/isolamento & purificação
11.
Biomed Pharmacother ; 118: 109152, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31376652

RESUMO

Antimicrobial peptides (AMPs) are small molecules with microbicidal and immunoregulatory activities. In this study we evaluated the anti-inflammatory and antimicrobial activities of peptides ToAP3 and ToAP4, AMPs from the venom of the Brazilian scorpion Tityus obscurus. To test the peptides' activity, murine bone marrow-derived macrophages (BMDMs) or dendritic cells (BMDCs) were stimulated with peptides plus LPS to analyze their ability to modulate cytokine release as well as phenotypic markers. For antimicrobial analysis, we evaluated the indirect activity against macrophage-internalized Cryptococcus neoformans and direct activity against Mycobacterium massiliense. Our data demonstrate that they were able to reduce TNF-α and IL-1ß transcript levels and protein levels for BMDM and BMDC. Furthermore, the reduction of TNF-α secretion, before LPS- inflammatory stimuli, is associated with peptide interaction with TLR-4. ToAP4 increased MHC-II expression in BMDC, while ToAP3 decreased co-stimulatory molecules such as CD80 and CD86. Although these peptides were able to modulate the production of cytokines and molecules associated with antigen presentation, they did not increase the ability of clearance of C. neoformans by macrophages. In antimicrobial analysis, only ToAP3 showed potent action against bacteria. Altogether, these results demonstrate a promising target for the development of new immunomodulatory and anti-bacterial therapies.


Assuntos
Anti-Infecciosos/farmacologia , Citocinas/metabolismo , Peptídeos/farmacologia , Venenos de Escorpião/química , Escorpiões , Receptor 4 Toll-Like/antagonistas & inibidores , Animais , Anti-Infecciosos/isolamento & purificação , Anti-Inflamatórios não Esteroides/isolamento & purificação , Anti-Inflamatórios não Esteroides/farmacologia , Linhagem Celular , Cryptococcus neoformans/efeitos dos fármacos , Células Dendríticas/efeitos dos fármacos , Células Dendríticas/imunologia , Imunidade Inata/efeitos dos fármacos , Macrófagos Peritoneais/efeitos dos fármacos , Macrófagos Peritoneais/imunologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Testes de Sensibilidade Microbiana , Mycobacterium abscessus/efeitos dos fármacos , Peptídeos/isolamento & purificação , Receptor 4 Toll-Like/genética
12.
Int J Mol Sci ; 20(15)2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31387229

RESUMO

Inflammatory bowel disease (IBD) is a worldwide healthcare problem calling for the development of new therapeutic drugs. Angelica sinensis and Zingiber officinale Roscoe are two common dietetic Chinese herbs, which are traditionally used for complementary treatment of gastrointestinal disorders. As bioactive constituents, volatile and pungent substances of these two herbs could be effectively extracted together by supercritical fluid extraction. In this study, the supercritical fluid extract of Angelica sinensis and Zingiber officinale Roscoe (AZ-SFE) was obtained by an optimized extraction process and it was chemically characterized. The anti-inflammatory effect and underlying mechanism of AZ-SFE were evaluated in a lipopolysaccharide (LPS)-induced RAW264.7 cell model and a 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis rat model. AZ-SFE notably inhibited the production of NO in LPS-stimulated macrophages, and it inhibited the proliferation of Concanavalin A (Con A)-induced splenocytes with suppression of the Th1 immune response. In vivo, the study demonstrated that AZ-SFE significantly alleviated disease activity, colonic shortening, macroscopic damage and histological injury of TNBS-treated rats with reduction of oxidative stress, suppression of inflammatory cytokines, and modulation of hepcidin and serum iron. These findings suggested that AZ-SFE may be a promising supplement for current IBD therapy.


Assuntos
Angelica sinensis/química , Anti-Inflamatórios não Esteroides/farmacologia , Colite/tratamento farmacológico , Gengibre/química , Extratos Vegetais/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Biomarcadores , Biópsia , Sobrevivência Celular/efeitos dos fármacos , Cromatografia com Fluido Supercrítico , Colite/etiologia , Colite/metabolismo , Citocinas/biossíntese , Modelos Animais de Doenças , Cromatografia Gasosa-Espectrometria de Massas , Mediadores da Inflamação/metabolismo , Lipopolissacarídeos/imunologia , Linfócitos/imunologia , Linfócitos/metabolismo , Camundongos , Óxido Nítrico/metabolismo , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Células RAW 264.7 , Ratos , Baço/imunologia , Baço/metabolismo , Ácido Trinitrobenzenossulfônico/efeitos adversos
13.
Chemosphere ; 237: 124431, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31374392

RESUMO

Degradation of naproxen, bisphenol S and ibuprofen in a hydroxylamine enhanced zero-valent copper (Cu0) catalyzed peroxymonosulfate system was investigated for the first time. We found that hydroxylamine addition accelerated the reduction of Cu2+ to Cu+ as well as the corrosion of Cu0, and environmental friendly gas nitrogen was the main product of hydroxylamine. Additionally, hydroxyl radical and sulfate radical were identified to be the dominant reaction species by competitive experiments. The degradation of naproxen, bisphenol S and ibuprofen kept highly efficient in the pH range of 3.0-7.0 in Cu0/hydroxylamine/peroxymonosulfate process, with their degradation products identified by HPLC-MS, which showed that Cu0/hydroxylamine/peroxymonosulfate system could be an alternative to remove non-steroidal antiinflammatory drugs or plasticizers in wastewater. Furthermore, the effects of Cu0, hydroxylamine and peroxymonosulfate dosage were studied and optimized by a BBD based response surface model. This study provided a method to solve the disadvantages of Cu0/peroxymonosulfate systems, and gave a promising method to enhance the efficiencies of ZVMs activated system such as iron, cobalt and copper.


Assuntos
Cobre , Hidroxilamina , Peróxidos , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/métodos , Anti-Inflamatórios não Esteroides/isolamento & purificação , Radical Hidroxila , Ibuprofeno/isolamento & purificação , Naproxeno/isolamento & purificação , Fenóis/isolamento & purificação , Plastificantes/isolamento & purificação , Sulfatos , Sulfonas/isolamento & purificação , Poluentes Químicos da Água/análise
14.
J Sep Sci ; 42(18): 2949-2956, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31267662

RESUMO

Passive sampling is an attractive technique for the long-term monitoring of pharmaceuticals in the water environment. The reliability of the received results depends on the properly performed calibration, namely the determination of analyte sampling rates. This step can be the source of a systematic error, as the sampling rate values are dependent on the water donor phase parameters. This is especially important for pharmaceuticals, since their chemical characteristics and ionic form change with pH. In this study, the cross-effect of pH (3, 7, and 9) and salinity (0, 7, and 35 practical salinity unit, using artificial sea water) on the passive sampling of 21 pharmaceuticals (antiparasitics, beta-blockers, non-steroidal anti-inflammatory drugs, sulfonamides) was tested. The primarily determined parameter was the sampling rate. In addition, the extraction efficiency, partitioning coefficient, and the concentration of the analytes on the sorbent were calculated. Generally, for the non-steroidal anti-inflammatory drugs, beta-blockers, and antiparasitics, the change both in pH and salinity had a negligible impact on the mentioned experimental parameters. In contrast, the extraction of sulfonamides was impacted by both pH and salinity, while lipophilicity was not a decisive parameter.


Assuntos
Antagonistas Adrenérgicos beta/isolamento & purificação , Anti-Inflamatórios não Esteroides/isolamento & purificação , Antiparasitários/isolamento & purificação , Preparações Farmacêuticas/isolamento & purificação , Sulfonamidas/isolamento & purificação , Poluentes Químicos da Água/isolamento & purificação , Antagonistas Adrenérgicos beta/química , Anti-Inflamatórios não Esteroides/química , Antiparasitários/química , Concentração de Íons de Hidrogênio , Preparações Farmacêuticas/química , Salinidade , Sulfonamidas/química , Poluentes Químicos da Água/química
15.
Mikrochim Acta ; 186(8): 534, 2019 07 16.
Artigo em Inglês | MEDLINE | ID: mdl-31312945

RESUMO

A nanohybrid was prepared from polyoxotungstate anion and graphene oxide (POT/GO) and characterized in terms of porosity by applying Fourier transform infrared and transmission electron microscopy. The nanohybrid was applied as a sorbent for the dispersive micro solid-phase extraction of the non-steroidal anti-inflammatory drugs (NSAIDs) ibuprofen, diclofenac, and naproxen. Different types of sorbents were compared, and the POT/GO nanohybrid was found to have the best adsorption affinity. The NSAIDs were quantified via HPLC with UV detection. Under the optimum conditions, the limits of detection (at an S/N ratio of 3) range between 0.02-0.03 ng.mL-1, and the linear response ranges extend from 0.08-200 ng.mL-1, respectively. The relative standard deviations (RSDs) for five replicates at three concentration levels (0.1, 5 and 100 ng.mL-1) of NSAIDs ranged from 4.1 to 6.1%. The applicability of the method was confirmed by analyzing spiked real water samples, and satisfactory results were obtained, with recoveries between 95.6 and 99.6%. Graphical abstract Schematic representation of the polyoxotungstate/graphene oxide nanohybrid preparation.


Assuntos
Anti-Inflamatórios não Esteroides/análise , Cromatografia Líquida de Alta Pressão/métodos , Grafite/química , Nanoestruturas/química , Adsorção , Anti-Inflamatórios não Esteroides/isolamento & purificação , Diclofenaco/análise , Diclofenaco/isolamento & purificação , Ibuprofeno/análise , Ibuprofeno/isolamento & purificação , Limite de Detecção , Naproxeno/análise , Naproxeno/isolamento & purificação , Polímeros/química , Porosidade , Extração em Fase Sólida , Espectrofotometria Ultravioleta , Compostos de Tungstênio/química , Poluentes Químicos da Água/análise
16.
J Chromatogr A ; 1603: 33-43, 2019 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-31248585

RESUMO

This study is the first to use a new ZSM-5 zeolite-based composite decorated with iron oxide magnetic nanoparticles and modified with hexadecyltrimethylammonium bromide surfactant (i.e., HDTMA-ZSM-5/Fe2O3) as an efficient sorbent for magnetic dispersive solid-phase extraction (MDSPE) of nonsteroidal anti-inflammatory drugs in water and urine samples with subsequent measurement by liquid chromatography diode array detection. Experimental factors affecting MDSPE were optimized using a multivariate optimization strategy. The optimum experimental conditions were: amount of sorbent, 40 mg; sample pH, 2.2; NaCl concentration, 2.5%; extraction time, 2 min; eluent solvent, methanol; eluent solvent volume, 424 µL; and elution time, 2 min. The linearity of the method was studied from 3.3 to 400 µg L-1 (N = 8) for ketoprofen, from 1.7 to 400 µg L-1 (N = 8) for felbinac, from 6.6 to 400 µg L-1 (N = 7) for diclofenac and from 9.9 to 400 µg L-1 (N = 6) for ibuprofen. Method repeatability was evaluated at 10 and 200 µg L-1 spiking levels, obtaining coefficients of variation between 2 and 5% (n = 6). Limits of detection, determined empirically, were 1.0 µg L-1 , 0.5 µg L-1, 2.0 µg L-1 and 3.0 µg L-1 for ketoprofen, felbinac, diclofenac and ibuprofen, respectively. Tap water, reservoir water, wastewater and five urine samples were selected to assess method applicability. Recovery values ranged between 86-107% and 80-112% for water and urine samples, respectively, showing negligible matrix effects. Finally, this method was employed to monitor ibuprofen excretion in real urine samples.


Assuntos
Anti-Inflamatórios não Esteroides/análise , Anti-Inflamatórios não Esteroides/isolamento & purificação , Compostos Férricos/química , Nanopartículas de Magnetita/química , Extração em Fase Sólida/métodos , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/isolamento & purificação , Zeolitas/química , Humanos , Limite de Detecção , Urinálise , Águas Residuárias/química
17.
Phytochemistry ; 164: 228-235, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31181354

RESUMO

Terminthia paniculata (Sanyeqi) is widely used for treating inflammation and rheumatic arthritis in the folk areas of Yunnan province, China. Its total extract was first revealed with xanthine oxidase (XO) inhibitory activity in vitro and anti-hyperuricemic effect in vivo. Bioassay-guided separation on Fr. A5 yielded six chalcone-flavonone heterodimers, termipaniculatones A-F. Their structures were elucidated based on extensive spectroscopic analyses involving HRESIMS, 1D and 2D NMR, UV, IR and [α]D, and the absolute configuration of termipaniculatone F was verified by ECD calculation. Termipaniculatones A and E showed obvious XO inhibitory activity with IC50 values of 55.6 and 89.5 µM, respectively, which took effects via a mix-type mode. A molecular modeling study revealed that termipaniculatone A was well located into the active site of XO by interacting with Glu802, Arg880, Thr1010 and Val1011 residues. Termipaniculatone A showed anti-hyperuricemic effects by decreasing serum uric acid levels and inhibiting XO activity in both serum and liver on potassium oxonate (PO)-induced hyperuricemia mice, and anti-inflammatory activity through alleviating paw swelling on monosodium urate (MSU)-induced mice, at the concentration of 20 mg/kg. This is the first time to reveal the anti-hyperuricemic and anti-acute gouty arthritis potency of T. paniculata and the characteristic biflavonoids as active constituents, which provides valuable information for searching new XO inhibitors from natural sources.


Assuntos
Anacardiaceae/química , Anti-Inflamatórios não Esteroides/farmacologia , Artrite Gotosa/tratamento farmacológico , Inibidores Enzimáticos/farmacologia , Hiperuricemia/tratamento farmacológico , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Artrite Gotosa/induzido quimicamente , Artrite Gotosa/metabolismo , Chalcona/química , Chalcona/isolamento & purificação , Chalcona/farmacologia , Relação Dose-Resposta a Droga , Edema/induzido quimicamente , Edema/tratamento farmacológico , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Flavanonas/química , Flavanonas/isolamento & purificação , Flavanonas/farmacologia , Hiperuricemia/induzido quimicamente , Hiperuricemia/metabolismo , Inflamação/induzido quimicamente , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos , Estrutura Molecular , Ácido Oxônico/antagonistas & inibidores , Relação Estrutura-Atividade , Ácido Úrico/antagonistas & inibidores , Xantina Oxidase/antagonistas & inibidores , Xantina Oxidase/metabolismo
18.
Phytochemistry ; 164: 206-214, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31177053

RESUMO

Eight undescribed cholestane glycosides named osaundersioside A-H, along with three previously known compounds named osaundersioside I-K were isolated from Ornithogalum saundersiae Baker bulbs (Asparagaceae). Their structures were elucidated by extensive spectroscopic analysis and chemical methods. All isolates were evaluated for their cytotoxic activity and inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide (NO) production. Osaundersioside C was thus determined to exhibit specific cytotoxicity towards MCF-7 cell line with an IC50 value of 0.20 µM, Osaundersioside H exhibited inhibitory effect on NO production in macrophages at the concentration of 10-5 M, with inhibition rate of 56.81%.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Asparagaceae/química , Colestanos/farmacologia , Glicosídeos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Proliferação de Células/efeitos dos fármacos , Colestanos/química , Colestanos/isolamento & purificação , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Glicosídeos/química , Glicosídeos/isolamento & purificação , Humanos , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Células MCF-7 , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Estrutura Molecular , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/biossíntese , Casca de Planta/química , Raízes de Plantas/química , Relação Estrutura-Atividade
19.
Carbohydr Polym ; 220: 43-52, 2019 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-31196549

RESUMO

An environmentally friendly and low-cost material based on cellulose filter paper modified with ß-cyclodextrin (ß-CD) was designed to uptake and elute drugs for water purification. To carry out the work, a ß-CD derivative was first obtained through reaction of ß-CD with N-(hydroxymethyl) acrylamide (NMA), and then ß-CD-NMA was grafted on cellulose by means of the Fenton's reaction. The CD-grafted cellulose paper was characterized by ATR-FTIR analysis, SEM images, and mechanical properties. CD-functionalized (F1) and non-functionalized (NF) papers were tested in aqueous media containing an antibiotic (ciprofloxacin) or a non-steroidal anti-inflammatory drug (diclofenac) covering a wide range of salinity levels. Ciprofloxacin was similarly adsorbed by both papers through ionic interactions, while diclofenac was selectively and remarkably captured by the CD-functionalized filter (up to 25 mg g-1 from saline medium under biorelevant conditions; ca. 60 mg g-1 Langmuir isotherm model). Effects of diclofenac concentration, volume of medium, and incubation time on the amount adsorbed were investigated in detail. Elution tests involved the combination of several organic solvents and alkaline solutions and revealed that acetonitrile:NaOH 10 mM aq. solution (50:50, v/v) allows for an effective recovery of the previously trapped diclofenac. Application of ultrasounds shortened the process to 10 min. Reusability of F1 papers was also evaluated. Overall, the CD-grafted cellulose paper appears as a suitable material for bioremediation and analytical purposes.


Assuntos
Anti-Inflamatórios não Esteroides/isolamento & purificação , Celulose/análogos & derivados , Ciclodextrinas/química , Diclofenaco/isolamento & purificação , Filtração/instrumentação , Poluentes Químicos da Água/isolamento & purificação , Purificação da Água/instrumentação , Água/análise , Anti-Inflamatórios não Esteroides/química , Biodegradação Ambiental , Ciprofloxacino/química , Ciprofloxacino/isolamento & purificação , Diclofenaco/química , Salinidade , Poluentes Químicos da Água/química
20.
Phytochemistry ; 164: 236-242, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31185420

RESUMO

Mangiterpenes A-C and 2',3'-seco-manginoid C, four undescribed sesquiterpene/monoterpene-shikimate-conjugated meroterpenoids with spiro ring systems, were isolated from Guignardia mangiferae. The structures and absolute configurations of these compounds were established by comprehensive spectroscopic analyses and electronic circular dichroism (ECD) calculations. Mangiterpenes A-C represent the first examples of sesquiterpene-shikimate-conjugated spirocyclic meroterpenoids, and 2',3'-seco-manginoid C features an unexpected 2',3'-seco-manginoids skeleton. Mangiterpene C strongly inhibited the production of NO inducted by LPS, with an IC50 value of 5.97 µM. It showed an anti-inflammatory effect by means of blocking in the NF-κB signaling pathway and decreasing the expression of inflammatory mediators.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Monoterpenos/farmacologia , Ácido Chiquímico/farmacologia , Compostos de Espiro/farmacologia , Terpenos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Relação Dose-Resposta a Droga , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Camundongos , Estrutura Molecular , Monoterpenos/química , Monoterpenos/isolamento & purificação , NF-kappa B/antagonistas & inibidores , NF-kappa B/metabolismo , Óxido Nítrico/antagonistas & inibidores , Óxido Nítrico/biossíntese , Células RAW 264.7 , Ácido Chiquímico/química , Ácido Chiquímico/isolamento & purificação , Transdução de Sinais/efeitos dos fármacos , Compostos de Espiro/química , Compostos de Espiro/isolamento & purificação , Relação Estrutura-Atividade , Terpenos/química , Terpenos/isolamento & purificação
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