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1.
Rev Cardiovasc Med ; 21(2): 275-287, 2020 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-32706215

RESUMO

Inflammation and oxidative stress are involved in the pathogenesis of cardiovascular diseases such as atherosclerosis, hypertension and ischemic heart disease. Natural products play an important role as nutritional supplements with potential health benefits in cardiovascular diseases. Polygonum minus (PM) is an aromatic plant that is widely used as a flavoring agent in cooking and has been recognized as a plant with various medicinal properties including antioxidative and anti-inflammatory actions. Phytoconstituents found in PM such as phenolic and flavonoid compounds contribute to the plant's antioxidative and anti-inflammatory effects. We conducted this review to systematically identify articles related to the antioxidative and anti-inflammatory activities of PM. A computerized database search was conducted on Ovid MEDLINE, PubMed, Scopus, and ACS publication, from 1946 until May 2020, and the following keywords were used: 'Kesum OR Polygonum minus OR Persicaria minor' AND 'inflammat* OR oxida* OR antioxida*'. A total of 125 articles were obtained. Another eight additional articles were identified through Google Scholar and review articles. Altogether, 17 articles were used for data extraction, comprising 16 articles on antioxidant and one article on anti-inflammatory activity of PM. These studies consist of 14 in vitro studies, one in vivo animal study, one combined in vitro and in vivo study and one combined in vitro and ex vivo study. All the studies reported that PM exhibits antioxidative and anti-inflammatory activities which are most likely attributed to its high phenolic and flavonoid content.


Assuntos
Anti-Inflamatórios/uso terapêutico , Antioxidantes/uso terapêutico , Flavonoides/uso terapêutico , Mediadores da Inflamação/antagonistas & inibidores , Inflamação/prevenção & controle , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Polygonum/química , Animais , Anti-Inflamatórios/efeitos adversos , Anti-Inflamatórios/isolamento & purificação , Antioxidantes/efeitos adversos , Antioxidantes/isolamento & purificação , Flavonoides/efeitos adversos , Flavonoides/isolamento & purificação , Humanos , Inflamação/metabolismo , Mediadores da Inflamação/metabolismo , Extratos Vegetais/efeitos adversos , Extratos Vegetais/isolamento & purificação , Transdução de Sinais
2.
Food Chem ; 332: 127390, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32603922

RESUMO

Revalorization of mushroom by-product (stalks of A. bisporus) by extracting its components is proposed. The extraction kinetics at 25 °C of ergosterol, phenolic compounds and antioxidant activity by mechanical agitation (at 130 rpm) and ultrasound assistance (at 182 and 321 W/L) in 70 and 96% v/v ethanol/water solutions during 30 min were evaluated and satisfactorily modelled, using the Weibull model (mean relative error ≤ 7.8%). The effect of the ethanol concentration was high in the ergosterol extraction yield (2 times higher yields in 96% than in 70%) but slight in those of phenolic compounds and antioxidant activity. Ultrasound assistance promoted considerable yield increases (up to 2 times higher in ergosterol, 46% in phenolic compounds and 25% in antioxidant activity) depending on the ethanol concentration and ultrasound power density. The residues after extraction were characterized and constituted a potential source of high value polysaccharides as ß-glucans (average 12.2 ± 1.7g/100 g dm).


Assuntos
Agaricales/química , Antioxidantes/química , Ergosterol/química , Extração em Fase Sólida/métodos , beta-Glucanas/química , Agaricales/metabolismo , Antioxidantes/isolamento & purificação , Ergosterol/isolamento & purificação , Etanol/química , Modelos Teóricos , Fenóis/química , Sonicação
3.
Food Chem ; 332: 127429, 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-32645678

RESUMO

Thermal processing is a traditional method for processing hawthorn into food or medicine. In this study, the compositions of free and bound phenolic compounds in raw hawthorn were analyzed by ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry, and the effect of thermal processing on phenolics and antioxidant activity was determined. Among the phenolics identified in unheated hawthorn, 26 were soluble, while only 10 were insoluble-bound. Thermal processing caused a significant reduction in total soluble phenolics content, but an increase in total insoluble-bound phenolics (p < 0.05). Procyanidin B2 and epicatechin showed the largest decreases in content, and were not detected in well-cooked hawthorn. The antioxidant activity also clearly decreased, with the chlorogenic acid, procyanidin B2, hyperoside, and isoquercetin contents correlating significantly (p < 0.05) with antioxidant activity. In general, the effect of thermal processes on phenolics and antioxidant activity was dependent on the types of phenolics and processing conditions.


Assuntos
Antioxidantes/química , Crataegus/química , Manipulação de Alimentos/métodos , Fenóis/química , Extratos Vegetais/química , Antioxidantes/isolamento & purificação , Cromatografia Líquida , Temperatura Alta , Fenóis/isolamento & purificação , Extratos Vegetais/isolamento & purificação
4.
J Chromatogr A ; 1625: 461302, 2020 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-32709345

RESUMO

Additives are added to polymers in small concentration to achieve desired application properties widely used to tailor the properties. The rapid diversification of their molecular structures, with often only minute differences, necessitates the development of adequate chromatographic techniques. While modified silica so far is the workhorse as stationary phase we have probed the potential of porous graphitic carbon (HypercarbTM) for this purpose. The results show that the multitude of physicochemical interactions between analyte molecules and the graphitic surface enables separations of polyolefin stabilizers with unprecedented selectivity. To support the chromatographic results the adsorption capability of HypercarbTM for selected antioxidants and UV absorbers has been determined by Raman spectroscopy and argon physisorption measurements. The shift of the Graphite-band in the Raman spectra of HypercarbTM upon infusion with additives correlates with the changes in the Adsorption Potential Distributions. The results of argon physisorption measurements go hand in hand with the chronology of desorption of the additives in liquid chromatography experiments. The elution sequence can be explained by van der Waals or London forces, π-π-interactions and electron lone pair donor-acceptor interactions between the graphite surface and analyte functional groups.


Assuntos
Grafite/química , Polímeros/química , Análise Espectral Raman , Adsorção , Antioxidantes/isolamento & purificação , Argônio/química , Clorofórmio/química , Éteres Metílicos/química , Polienos/química , Porosidade , Fatores de Tempo
5.
J Chromatogr A ; 1623: 461150, 2020 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-32505270

RESUMO

Antioxidants play an essential role in human health, as they have been found to be capable of lowering the incidence of many diseases, such as cancer and angiocardiopathy. Currently, more attention is paid to natural antioxidants because of the possible insecurity of synthetic antioxidants. Thus, the development of efficient techniques or methods to separate antioxidants from natural sources is requested urgently. High-speed counter current chromatography (HSCCC) is a unique support-free liquid-liquid chromatographic technique and has been widely applied in the field of separation of natural products. In this review, we summarize and analyze the related researches on the application of HSCCC in the separation of various natural antioxidants so far. The purpose of the article is to provide a certain theoretical support for the separation of natural antioxidants by HSCCC, and to make full use of advantages of HSCCC in the separation of bioactive components. In particular, some key problems associated with the separation strategies, the structural categories of natural antioxidants, solvent system choices, and the application of different elution modes in HSCCC separation, are summarized and commented. We expect that the content reviewed can offer more evidence for the development of the field of natural antioxidants separation, so as to achieve large-scale preparation of natural antioxidants.


Assuntos
Antioxidantes/isolamento & purificação , Distribuição Contracorrente/métodos , Compostos de Bifenilo/química , Cromatografia Líquida de Alta Pressão , Humanos , Concentração de Íons de Hidrogênio , Picratos/química , Solventes/química
6.
PLoS One ; 15(6): e0234661, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32544201

RESUMO

Xanthoxylin was the main compound (content 44.92% of total volatiles) in the leaves of Luodian B. balsamifera, which might be the key cause of failure in collecting essential oil (EO) of the leaves using general hydrodistillation in Clevenger apparatus. A modified hydrodistillation equipped with Clevenger apparatus was designed for isolating EO from the leaves. Six EOs of Luodian B. balsamifera harvested once a month from September to next February were collected successfully. The main components of EOs were δ-elemene, α-cubenene, caryophyllene, caryophyllene epoxide, γ-eudesmol, xanthoxylin, and α-eudesmol. The EOs of Luodian B. balsamifera collected from October to December had higher antioxidant activities (ACs). Combining the principal component analysis of chemical components with the results of ACs and the yields of six EOs, the leaves of Luodian B. balsamifera were suitable to be harvested in November and December to obtain EO with high quality.


Assuntos
Antioxidantes/isolamento & purificação , Asteraceae/química , Óleos Voláteis/química , Antioxidantes/farmacologia , China , Destilação/instrumentação , Destilação/métodos , Óleos Voláteis/farmacologia , Folhas de Planta/química , Óleos Vegetais/química , Óleos Vegetais/farmacologia , Sesquiterpenos Policíclicos/isolamento & purificação , Estações do Ano , Sesquiterpenos/isolamento & purificação
7.
Proc Natl Acad Sci U S A ; 117(27): 15554-15564, 2020 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-32561649

RESUMO

The serum haptoglobin protein (Hp) scavenges toxic hemoglobin (Hb) leaked into the bloodstream from erythrocytes. In humans, there are two frequently occurring allelic forms of Hp, resulting in three genotypes: Homozygous Hp 1-1 and Hp 2-2, and heterozygous Hp 2-1. The Hp genetic polymorphism has an intriguing effect on the quaternary structure of Hp. The simplest form, Hp 1-1, forms dimers consisting of two α1ß units, connected by disulfide bridges. Hp 2-1 forms mixtures of linear (α1)2(α2)n-2(ß)n oligomers (n > 1) while Hp 2-2 occurs in cyclic (α2)n(ß)n oligomers (n > 2). Different Hp genotypes bind Hb with different affinities, with Hp 2-2 being the weakest binder. This behavior has a significant influence on Hp's antioxidant capacity, with potentially distinctive personalized clinical consequences. Although Hp has been studied extensively in the past, the finest molecular details of the observed differences in interactions between Hp and Hb are not yet fully understood. Here, we determined the full proteoform profiles and proteoform assemblies of all three most common genetic Hp variants. We combined several state-of-the-art analytical methods, including various forms of chromatography, mass photometry, and different tiers of mass spectrometry, to reveal how the tens to hundreds distinct proteoforms and their assemblies influence Hp's capacity for Hb binding. We extend the current knowledge by showing that Hb binding does not just depend on the donor's genotype, but is also affected by variations in Hp oligomerization, glycosylation, and proteolytic processing of the Hp α-chain.


Assuntos
Haptoglobinas/genética , Hemoglobinas/metabolismo , Alelos , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/metabolismo , Glicosilação , Haptoglobinas/química , Haptoglobinas/isolamento & purificação , Haptoglobinas/metabolismo , Hemoglobinas/toxicidade , Humanos , Espectrometria de Massas , Modelos Moleculares , Estrutura Molecular , Polimorfismo Genético , Ligação Proteica , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Isoformas de Proteínas/isolamento & purificação , Isoformas de Proteínas/metabolismo , Relação Estrutura-Atividade
8.
PLoS One ; 15(6): e0234435, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32574221

RESUMO

This study was designed to investigate the antioxidant properties of the extracts and subfractions of various polarities from Clerodendrum cyrtophyllum Turcz leaves and the related phenolic compound profiles. The ethyl acetate fraction (EAF) showed the most potent radical-scavenging activity for DPPH radicals, ABTS radicals, and superoxide anion (O2·-) radicals as well as the highest reducing power of the fractions tested; the n-butyl alcohol fraction (BAF) was the most effective in scavenging hydroxyl radical (OH·), and the dichloromethane fraction (DMF) exhibited the highest ferrous ion chelating activity. Twelve phenolic components were identified from the EAF of C. cyrtophyllum. Additionally, acteoside (1) was found to be a major component (0.803 g, 0.54%) and show DPPH and ABTS radical scavenging activities with IC50 values of 79.65±3.4 and 23.00±1.5 µg/ml, indicating it is principally responsible for the significant total antioxidant effect of C. cyrtophyllum. Our work offers a theoretical basis for further utilization of C. cyrtophyllum as a potential source of natural, green antioxidants derived from plants.


Assuntos
Antioxidantes/farmacologia , Clerodendrum/química , Glucosídeos/farmacologia , Fenóis/farmacologia , Extratos Vegetais/farmacologia , Antioxidantes/análise , Antioxidantes/isolamento & purificação , Glucosídeos/análise , Glucosídeos/isolamento & purificação , Radical Hidroxila/metabolismo , Fenóis/análise , Fenóis/isolamento & purificação , Extratos Vegetais/análise , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Superóxidos/metabolismo
9.
Food Chem ; 330: 127156, 2020 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-32531631

RESUMO

Rice bran (RB) and wheat bran (WB) fermented with L. plantarum 423 had enhanced odor intensity, especially for sulfides and aromatics. The hydroxyl radical-scavenging activity (73.28 ± 3.18%) and oxygen radical-scavenging activity (2.12 ± 0.08 mmol·TE/g) of RB fermentation broth were better than those of WB fermentation broth. Even at 2 µg/ml, the purified antioxidant fractions from the WB fermentation broth showed strong intracellular ROS-scavenging activity in human umbilical vein endothelial cells (HUVECs), and the purified antioxidant fractions (200 µg/ml) from the RB fermentation broth had a good antiaging effect. The dominant antioxidant components in the RB and WB fermentation broths were acids (70.21%) and ketones (10.64%), these components jointly give the RB and WB fermentation broths a variety of antioxidant properties. These results are beneficial for developing RB and WB deep-processing technology and laid the foundation for the preparation of antioxidant fractions with L. plantarum 423.


Assuntos
Antioxidantes/farmacologia , Fibras na Dieta/metabolismo , Fermentação , Aromatizantes/análise , Lactobacillus plantarum/metabolismo , Oryza/metabolismo , Triticum/metabolismo , Antioxidantes/isolamento & purificação , Células Cultivadas , Nariz Eletrônico , Células Endoteliais/efeitos dos fármacos , Aromatizantes/isolamento & purificação , Humanos , Oryza/química , Triticum/química
10.
PLoS One ; 15(5): e0233088, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32401824

RESUMO

Microglial activation can release free radicals and various pro-inflammatory cytokines, which implicates the progress of a neurodegenerative disease. Therefore suppression of microglial activation can be an appropriate strategy for combating neurodegenerative diseases. Betanin is a red food dye that acts as free radical scavenger and can be a promising candidate for this purpose. In this study, purification of betanin from red beetroots was carried out by normal phase colum chromatography, yielding 500 mg of betanin from 100 g of red beetroot. The purified betanin was evaluated by TLC, UV-visible, HPLC, ESI-MASS, FT-IR spectroscopy. Investigation on the inhibitory effect of betanin on activated microglia was performed using primary microglial culture. The results showed that betanin significantly inhibited lipopolysaccharide induced microglial function including the production of nitric oxide free radicals, reactive oxygen species, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-1 beta (IL-1ß). Moreover, betanin modulated mitochondrial membrane potential, lysosomal membrane permeabilization and adenosine triphosphate. We further investigated the interaction of betanin with TNF-α, IL-6 and Nitric oxide synthase (iNOS or NOS2) using in silico molecular docking analysis. The docking results demonstrated that betanin have significant negative binding energy against active sites of TNF-α, IL-6 and iNOS.


Assuntos
Anti-Inflamatórios/farmacologia , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Beta vulgaris/química , Betacianinas/isolamento & purificação , Betacianinas/farmacologia , Lipopolissacarídeos/farmacologia , Microglia/efeitos dos fármacos , Animais , Sobrevivência Celular/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Citometria de Fluxo , Microglia/metabolismo , Simulação de Acoplamento Molecular , Ratos , Ratos Wistar
11.
Food Chem ; 327: 127059, 2020 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-32447138

RESUMO

The aim of this study was to purify and identify antioxidant peptides from watermelon seed protein hydrolysates (WSPHs-I: Mw < 1 kDa) and further evaluate their cytoprotective effects against H2O2-induced oxidative stress in HepG2 cells. After purification by Sephadex G-15 and semi-preparative reversed-phase high performance liquid chromatography (RP-HPLC), five peptides, RDPEER (P1), KELEEK (P2), DAAGRLQE (P3), LDDDGRL (P4), and GFAGDDAPRA (P5) were sequenced by LC-MS/MS and synthesized with solid-phase synthesis method. These peptides showed desirable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity (IC50: 0.216 ± 0.01-0.435 ± 0.03), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity (IC50: 0.54 ± 0.02-1.23 ± 0.03), and oxygen radical absorbance capacity (ORAC) (82.36 ± 1.2-130.67 ± 2.2 µM TE/mg). Among them, peptide P1 exhibited the strongest antioxidant capacity. Moreover, the results suggested that peptide P1 may protect HepG2 cells from H2O2-induced oxidative damage by significantly inhibiting reactive oxygen species (ROS), [Ca2+]i, malondialdehyde (MDA) levels and increasing antioxidative enzyme activities.


Assuntos
Citrullus/química , Peróxido de Hidrogênio/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Peptídeos/química , Hidrolisados de Proteína/química , Sequência de Aminoácidos , Antioxidantes/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Cromatografia Líquida de Alta Pressão , Citrullus/metabolismo , Células Hep G2 , Humanos , Oxirredução , Peptídeos/isolamento & purificação , Peptídeos/farmacologia , Proteínas de Plantas/metabolismo , Substâncias Protetoras/química , Substâncias Protetoras/isolamento & purificação , Substâncias Protetoras/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Sementes/química , Sementes/metabolismo , Relação Estrutura-Atividade , Espectrometria de Massas em Tandem
12.
J Food Sci ; 85(6): 1735-1741, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32468582

RESUMO

In this study, the antioxidant activity of mungbean protein hydrolysate (MPH) was systematically investigated. MPH was fractionated by ultrafiltration into two major fractions (MPH-1 <3 kDa, MPH-2 >3 kDa). Fraction MPH-1, which exhibited the highest antioxidant activity, was further fractionated by gel column into three fractions (MPH-1A, MPH-1B, and MPH-1C). The antioxidant activity of the MPH-1B fraction was stronger than that of the other fractions. Eight mungbean peptides (P1-P8) were identified in fraction MPH-1B by UPLC-Q-TOF-MS. Among them, peptides Trp-Gly-Asn (WGN, P2), Ala-Trp (AW, P4), Arg-Gly-Trp-Tyr-Glu (RGWYE, P5), and Gly-Val-Pro-Phe-Trp (GVPFW, P7) had high antioxidant activity. Moreover, these four peptides exerted protective effects against H2 O2 -induced cytotoxicity and regulated the MDA content, CAT activity, and total GSH content in HepG2 cells with specific observation. This study demonstrated the potential of MPH as a source of antioxidant peptides. This provides a scientific basis for the preparation of antioxidant peptides from mungbean protein. PRACTICAL APPLICATION: This study demonstrated the potential of the hydrolysate of mungbean protein as a source of antioxidant peptides and provided a scientific basis for the preparation of antioxidant peptides from mungbean protein.


Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Proteínas de Plantas/química , Vigna/química , Sequência de Aminoácidos , Animais , Antioxidantes/isolamento & purificação , Sobrevivência Celular/efeitos dos fármacos , Células Hep G2 , Humanos , Peptídeos/química , Proteínas de Plantas/farmacologia , Hidrolisados de Proteína/química , Hidrolisados de Proteína/farmacologia
13.
Braz J Med Biol Res ; 53(5): e9303, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32348424

RESUMO

The control of dyslipidemia using plants is an important subject of studies since it has numerous benefits in cardiovascular protection. The objective of this study was to evaluate the effect of three Camellia sinensis L. teas (green, red, and white) on left ventricular hypertrophy and insulin resistance in low-density lipoprotein receptor knockout (LDLr-/-) mice fed a high-fat diet. The LDLr-/- mice were divided into four experimental groups: Group C: standard feed; Group CT: standard feed and three teas, Group HL: high-fat feed; HLT Group: high-fat feed and three teas. The three types of tea (green, red, and white) originated from different processing of the Camellia sinensis L. plant, and were administered associated once a day at a dose of 25 mg/kg by gavage for 60 days. The teas partially prevented hyperlipidemia, the decrease of the serum levels of high-density lipoproteins (HDL), insulin resistance, and increased C-reactive protein (CRP) levels, and completely prevented left ventricular hypertrophy in LDLr -/- mice of the HLT group. In conclusion, the three Camellia sinensis L. teas used to control genetic dyslipidemia associated with a high-fat diet can be used as an auxiliary treatment associated with the control of lipid intake, thus promoting cardiac protection against hyperlipidemia.


Assuntos
Antioxidantes/administração & dosagem , Camellia sinensis/química , Dislipidemias/tratamento farmacológico , Hipertrofia Ventricular Esquerda/tratamento farmacológico , Resistência à Insulina , Extratos Vegetais/administração & dosagem , Chá , Animais , Antioxidantes/isolamento & purificação , Masculino , Camundongos
14.
Chemosphere ; 253: 126728, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32298913

RESUMO

Nile perch wastewater was biodegraded using two Bacillus species to recover bioactive substances to enhance its reutilization value. The two Bacillus species successfully produced low-molecular-weight substances with a 47.8% degree of hydrolysis. The antioxidant activities of the Nile perch wastewater increased as the biodegradation proceeded, and the culture supernatant exhibited the highest DPPH (80.1%), ABTS (93.1%) and Fe2+ chelating (88.5%) antioxidant activities at 60 h. The antioxidant potential of the biodegraded Nile perch wastewater was found to be higher than those of other fish hydrolysates. Moreover, the biodegraded Nile perch wastewater exhibited effective antimicrobial activity against Vibrio vulnificus, exhibiting a minimal inhibitory concentration of 585 µg mL-1. Two-dimensional thin layer chromatography analysis revealed the specific amino acids responsible for the antioxidant activity, and molecular-weight cut-off ultrafiltration revealed that the <2-kDa fraction exhibited the highest antioxidant activity with the lowest IC50 values (0.43 and 0.22 mg mL-1 for DPPH and ABTS antioxidant activities, respectively). This is the first report of the reutilization of Nile perch wastewater as a natural antioxidant and antimicrobial ingredient for nutraceuticals.


Assuntos
Antibacterianos/isolamento & purificação , Antioxidantes/isolamento & purificação , Bacillus/metabolismo , Peptídeos/isolamento & purificação , Percas , Águas Residuárias/química , Aminoácidos/isolamento & purificação , Aminoácidos/farmacologia , Animais , Antibacterianos/farmacologia , Antioxidantes/farmacologia , Biodegradação Ambiental , Compostos de Bifenilo/química , Quelantes/isolamento & purificação , Quelantes/farmacologia , Pesqueiros , Hidrólise , Peso Molecular , Peptídeos/farmacologia , Percas/crescimento & desenvolvimento , Picratos/química , Ultrafiltração , Vibrio vulnificus/efeitos dos fármacos
15.
High Blood Press Cardiovasc Prev ; 27(3): 251-258, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32266706

RESUMO

INTRODUCTION: Ischemic heart disease is closely associated with many risk factors. Germinated brown rice extract (GBR) has potent antioxidant activities for alleviating the factors for developing heart failure such as hypertension and diabetes mellitus. AIM: The objective of the present study was to determine the cardio-protective effects of GBR and to elucidate the mechanisms underlying these effects in a model of simulated myocardial ischemic/ reperfusion injury (sI/R). METHODS: An in vitro study was performed on cultured rat cardiomyoblasts (H9c2) exposed to sI/R. The expression of apoptosis and signaling proteins was assessed using Western blot analyses. Eighteen New Zealand White rabbits were divided into 3 groups and the left circumflex coronary artery was ligated to induce myocardial ischemia. Heart functions were monitored by electrocardiography and echocardiography 0, 30, and 60 days after coronary artery ligation. RESULTS: GBR consumption group showed significantly improved cardiac function and reduced the heart rate, along with reduced mean arterial pressure and plasma glucose level. Also, GBR showed good scavenging activity, pretreatment with GBR inhibited I/R induced apoptosis by suppressing the production of caspase 3 and p38 MAPK. CONCLUSIONS: These results suggest that intake of germinated brown rice may effectively to protect cell proliferation and apoptosis and may provide important nutrients to prevent heart failure due to myocardial ischemia.


Assuntos
Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Infarto do Miocárdio/prevenção & controle , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Miócitos Cardíacos/efeitos dos fármacos , Oryza/microbiologia , Extratos Vegetais/farmacologia , Animais , Antioxidantes/isolamento & purificação , Caspase 3/metabolismo , Linhagem Celular , Modelos Animais de Doenças , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Fermentação , Hemodinâmica/efeitos dos fármacos , Infarto do Miocárdio/metabolismo , Infarto do Miocárdio/patologia , Infarto do Miocárdio/fisiopatologia , Traumatismo por Reperfusão Miocárdica/metabolismo , Traumatismo por Reperfusão Miocárdica/patologia , Traumatismo por Reperfusão Miocárdica/fisiopatologia , Miócitos Cardíacos/metabolismo , Miócitos Cardíacos/patologia , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Coelhos , Ratos , Espécies Reativas de Oxigênio/metabolismo , Transdução de Sinais , Função Ventricular Esquerda/efeitos dos fármacos , Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
16.
J Food Sci ; 85(5): 1500-1512, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32267966

RESUMO

Olive pomace is considered a solid by-product and a rich source of valuable compounds such as polyphenols, flavonoids with antioxidant properties, and proteins. Nonthermal technologies, which cause alterations to cell permeability, are being explored to assist conventional recovery techniques. The aim of this study was to assess the effect of pulsed electric fields (PEF) and high pressure (HP) on improved recovery yield of the high-added-value compounds or to shorten the extraction time of these compounds. Olive pomace (Tsounati cv) was pretreated with PEF (1.0 to 6.5 kV/cm, 0.9 to 51.1 kJ/kg, and 15 µs pulse width) or HP (200 to 600 MPa and 0 to 40 min). Evaluation of the intracellular compounds extracted via solid-liquid extraction (50% ethanol-water solution) was performed. More intense PEF and HP conditions resulted in a significant increase of the phenolic concentration up to 91.6% and 71.8%, respectively. The increased antioxidant capacity of each extract was correlated to phenolic compound concentration. The protein concentration that was achieved with PEF pretreatment was doubled; however, HP-pretreated extracts reached 88.1% higher yield than untreated for pressures up to 200 MPa. HP and PEF pretreatment decreased extraction completion time t98 (needed time to recover the equal amount of phenolics and proteins of untreated after 60 min of conventional extraction) to 12 min and lower than 1 min, respectively. To conclude, both pretreatments are effective in improving the conventional extraction process for increased yield recovery of high-added-value compounds from olive pomace.


Assuntos
Manipulação de Alimentos/métodos , Olea/química , Extratos Vegetais/isolamento & purificação , Resíduos/análise , Antioxidantes/análise , Antioxidantes/isolamento & purificação , Eletricidade , Flavonoides/análise , Flavonoides/isolamento & purificação , Manipulação de Alimentos/instrumentação , Frutas/química , Extratos Vegetais/análise , Polifenóis/análise , Polifenóis/isolamento & purificação
17.
Food Chem ; 318: 126484, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32151923

RESUMO

The phytochemical contents, peroxyl radical scavenging capacities (PSCs) and cellular antioxidant activities (CAAs) of free and bound fractions of rice were reported. Black rice had the highest total phenolic content and total flavonoid content in free and bound fractions, followed by red rice, brown rice, and polished rice. Black rice contained much more free phenolic compounds than other rice samples, such as cyanidin-3-O-glucoside, protocatechuic acid, and vanillic acid. Tocopherols and tocotrienols contents were highest in red rice, then in black rice, brown rice, and polished rice. PSCs and CAAs of free and bound fractions were in the order: black rice > red rice > brown rice > polished rice, except that bound CAA of red rice was higher than that of black rice. The cellular uptake rate of free phenolics was highest in red rice, while cellular uptake rates of bound phenolics were highest in brown rice and polished rice.


Assuntos
Antioxidantes/análise , Carcinoma Hepatocelular/tratamento farmacológico , Flavonoides/análise , Neoplasias Hepáticas/tratamento farmacológico , Oryza/química , Fenóis/análise , Compostos Fitoquímicos/análise , Amidinas/efeitos adversos , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Depuradores de Radicais Livres/metabolismo , Células Hep G2 , Humanos , Estresse Oxidativo/efeitos dos fármacos , Peróxidos/metabolismo , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Especificidade da Espécie , Tocoferóis/análise , Tocotrienóis/análise
18.
J Food Sci ; 85(4): 1060-1069, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32147838

RESUMO

Inonotus obliquus is a traditional mushroom well known for its therapeutic value. In this study, various solvent fractions of I. obliquus were preliminarily screened for their antioxidant, α-amylase and α-glucosidase inhibition properties. To improve the drug delivery, the active fraction (ethyl acetate fraction) of I. obliquus was synthesized into fungisome (ethyl acetate phophotidyl choline complex, EAPC) and its physical parameters were assessed using Fourier transform infrared spectroscopy (FTIR), High performance liquid chromatography (HPLC), Scanning electron microscope (SEM), and ς potential analysis. Then normal human hepatic L02 cells was used to evaluate the cytotoxicity of EAPC. The results showed that EA fraction possesses significant free radical scavenging, α-amylase and α-glucosidase inhibition properties. FTIR, SEM, and HPLC analysis confirmed the fungisome formation. The particle size of EAPC was 102.80 ± 0.42 nm and the ς potential was -54.30 ± 0.61 mV. The percentage of drug entrapment efficiency was 97.13% and the drug release rates of EAPC in simulated gastric fluid and simulated intestinal fluid were 75.04 ± 0.29% and 93.03 ± 0.36%, respectively. EAPC was nontoxic to L02 cells, however it could selectively fight against the H2 O2 induced oxidative damage in L02 cells. This is the first study to provide scientific information to utilize the active fraction of I. obliquus as fungisome. PRACTICAL APPLICATIONS: Inonotus obliquus (IO) is a traditional medicinal fungus. The extracts of IO have obvious antioxidant and hypoglycemic activities. Ethyl acetate (EA) fraction of IO was encapsulated in liposomes to form EAPC. EAPC has a sustained-release effect. It has nontoxic to L02 cells and could protect L02 cells from oxidative damage caused by hydrogen peroxide. This study could provide new ideas for the treatment of diabetes.


Assuntos
Agaricales/química , Antioxidantes/farmacologia , Basidiomycota/química , Inibidores Enzimáticos/farmacologia , Peróxido de Hidrogênio/toxicidade , Extratos Vegetais/farmacologia , alfa-Amilases/antagonistas & inibidores , Antioxidantes/química , Antioxidantes/isolamento & purificação , Linhagem Celular , Inibidores Enzimáticos/química , Inibidores Enzimáticos/isolamento & purificação , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Humanos , Hipoglicemiantes/química , Hipoglicemiantes/isolamento & purificação , Hipoglicemiantes/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , alfa-Amilases/química , alfa-Glucosidases/química
19.
Food Chem ; 319: 126534, 2020 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-32193058

RESUMO

The antioxidant peptides extracted from duck plasma hydrolysate (DPH) was investigated. The antioxidant activity of DPH, which was isolated and purified via ultrafiltration, size exclusion chromatography, and reversed-phase high-performance liquid chromatography, was evaluated using its free radical scavenging ability. Nano-liquid chromatography-tandem mass spectrometry was conducted to identify the DPH fractions with the highest antioxidant ability. Seven novel peptides: LDGP, TGVGTK, EVGK, RCLQ, LHDVK, KLGA, and AGGVPAG (400.43, 561.63, 431.48, 260.14, 610.71, 387.47, and 527.57 Da, respectively) were identified and synthesized using a solid-phase peptide produce to evaluate their antioxidant activities. Of these, EVGK exhibited the highest Fe2+ chelating ability (16.35%), and RCLQ presented the highest reducing power, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt scavenging activity, and 1,1-diphenyl-2-picrylhydrazyl scavenging rate (0.62, 274.83 mM TE/mg, and 95.12%, respectively). Our results indicated that DPH possessed antioxidant capabilities and could be used to obtain antioxidant peptides, thus adding economic value to duck blood.


Assuntos
Antioxidantes/química , Proteínas Sanguíneas/química , Patos , Peptídeos/química , Animais , Antioxidantes/isolamento & purificação , Compostos de Bifenilo , Proteínas Sanguíneas/isolamento & purificação , Cromatografia em Gel , Peptídeos/isolamento & purificação , Hidrolisados de Proteína/química
20.
Phytother Res ; 34(9): 2170-2179, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32189392

RESUMO

The purpose of this review is to examine epigallocatechin-3-gallate (EGCG) regarding its stability in different conditions (pH-value, concentration, temperature), its interactions with common cosmetic ingredients, and its application in the dermatological field. The literature research considered published journal articles (clinical trials and scientific reviews). Studies were identified by searching electronic databases (MEDLINE and PubMed) and reference lists of respective articles. Higher concentrations of EGCG were reported to correlate with better stability and the same can be said for low temperatures and pH values. The interaction between EGCG and hyaluronic acid strengthens its antioxidant activities. Titanium dioxide coated with EGCG proved a suitable ingredient in sunscreens. The polyphenol possesses antioxidant properties, which proved effective in the prevention of UV-induced skin damage and to alleviate the symptoms of Imiquimod-induced psoriasis. The three endpoints of this review not only showed interesting results but also highlighted some limitations of EGCG. Studies show that the molecule is unstable, which may hinder its dermatological and cosmetic applications. The reported interactions with cosmetic ingredients were limited. As the health aspects of EGCG are well-reported, ECGC has become a focus of interest for health professionals trying to treat common dermatological diseases.


Assuntos
Antioxidantes/administração & dosagem , Catequina/análogos & derivados , Dermatologia/métodos , Extratos Vegetais/administração & dosagem , Chá/química , Administração Tópica , Antioxidantes/química , Antioxidantes/isolamento & purificação , Fenômenos Biofísicos , Catequina/administração & dosagem , Catequina/química , Catequina/isolamento & purificação , Cosméticos , Dermatologia/tendências , Humanos , Imiquimode/uso terapêutico , Psoríase/tratamento farmacológico
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